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Persistent environmental contaminants in fish and wildlife

The publication of Silent Spring (Carson 1962) highlighted the potential for dichlorodiphenyl trichloroethane (DDT) and other pesticides that persist in the environment to accumulate in and to harm fish, wildlife, and the ecosystems upon which they depend. The federal government responded in the mid-1960's by establishing a multi-agency program to monitor the concentrations of pesticides and, later, other long-lived toxic contaminants in all segments of the environment. The U.S. Fish and Wildlife Service (USFWS) participated in this program by periodically measuring contaminant concentrations in freshwater fish and birds (Johnson et al. 1967). Fish were selected for monitoring aquatic ecosystems because of their tendency to accumulate pesticides and other contaminants. The European starling ( Sturnus vulgaris ) was selected for monitoring contaminant levels in terrestrial habitats because of its varied diet and wide geographic distribution. Following a successful pilot study (Heath and Prouty 1967), the wings of hunter-killed ducks were used to monitor contaminants in duck populations of the major flyways, and thereby to also provide an assessment of contaminant levels in wetlands. The USFWS maintained this National Contaminant Biomonitoring Program into the 1980's, with the objective of continuing the documentation of temporal and geographic trends in contaminant concentrations (Prouty and Bunck 1986; Bunck et al. 1987; Schmitt and Brumbaugh 1990; Schmitt et al. 1990).

Book chapter

Distribution of chlorinated volatile organic compounds and per- and polyfluoroalkyl substances in groundwater and surface water at the former Naval Air Warfare Center, West Trenton, New Jersey, 2018

Groundwater wells and surface-water storm sewers contaminated with volatile organic compounds (VOCs) and per- and polyfluoroalkyl substances (PFASs) at the former Naval Air Warfare Center (NAWC) site in West Trenton, New Jersey were sampled in 2018 as part of the Navy’s long-term monitoring program. Trichloroethene (TCE), cis-1,2-dichloroethene (cisDCE), and vinyl chloride concentrations were plotted in map view and selected cross sections to elucidate the vertical and horizontal extent and distribution of contamination, along with a tabular comparison between 2018 and previous analytical results. The 2018 data showed that the areas of VOC contamination (>1 microgram per liter) decreased slightly on the north and east sides of the NAWC site from previous sampling dates; these decreases are attributed to the influence of the pump-and-treat system, natural attenuation processes, and various engineered bioaugmentation experiments that have occurred onsite. Off-site groundwater samples indicate the VOC contaminated groundwater is likely hydraulically constrained by the pump-and-treat system and appears to not be moving offsite to the south and west of NAWC. Only one offsite well, 50BR, located along the eastern margin of the site, was found to have detectable TCE and cisDCE concentrations, indicating that VOC contamination continues to migrate a short distance offsite to the east. Detectable VOC contamination was found in wells as deep as 200 and 221 feet on both the east and west sides of the NAWC site. Comparisons of present-day data to data from past sampling efforts indicate that TCE concentrations in most wells have decreased slowly over time. Results from surface-water samples indicate that VOCs enter surface water predominantly through the West Ditch drainage system. Concentrations and fluxes of VOCs are higher when groundwater levels are higher, indicating contaminated groundwater discharges into the surface water system. Higher VOC concentrations at the Interceptor site relative to other sites in the West Ditch indicate the contamination in the West Ditch system is likely caused by contaminated groundwater discharging to the West Ditch storm sewer near manhole MH-140 when water table levels are high. The pump-and-treat extraction wells at the former NAWC site were sampled for per- and polyfluoroalkyl substances (PFAS) in 2018. The suite of reported PFAS include perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid, and perfluorobutane sulfonate. Concentrations were plotted in map view to determine the areal extent of the PFAS contamination at the site. Extraction well 48BR sampled on the eastern half of the site was found to have PFOS and PFOA concentrations greater than the New Jersey Department of Environmental Protection Drinking Water maximum contaminant levels (MCLs), which is consistent with the distribution of highest PFAS concentrations in surface water in the OF-4 storm sewer system that drains that area, as well as previously collected PFAS concentrations in monitoring wells. On the western half of the site, the extraction well 08BR sample exceeded MCLs for PFOA and PFOS and the extraction well 22BR sample exceeded the MCL for PFOA, but samples from all other extraction wells were below the MCLs or other criteria for all PFAS analyzed. Concentrations of PFOA exceeded concentrations of PFOS on the west side of NAWC in both groundwater and surface water, which contrasts with the conditions on the east side of NAWC where PFOS concentrations exceeded PFOA concentrations. However, this observation was based on a limited number of samples on the west side of NAWC from 2018 and previous years, so more PFAS sampling is needed on the west side to assess this further.

New Jersey

Spatial and temporal variability of nutrients and algae in the Republican River and Milford Lake, Kansas, June through November 2017 and May through November 2018

Milford Lake has been listed as impaired and designated hypereutrophic because of excessive nutrient loading, specifically biologically available orthophosphate. It is the largest lake by surface area in Kansas and is a reservoir built for purposes including water supply and recreation. In 2015, the Kansas Department of Health and Environment (KDHE) divided the lake into three zones (Zones A, B, and C) for recreational monitoring of harmful algal blooms (HABs). Upstream Zone C has historically been more affected by HABs than Zones B and A, and Zone C has historically had the highest phosphorus concentrations. The U.S. Geological Survey, in cooperation with the KDHE, completed a study in 2017–18 to assess the spatial and temporal variability of nutrients and algae in the Republican River (the primary inflow to Milford Lake) and Milford Lake using spatially and temporally dense data. During the study period, discrete water-quality samples were collected at 36 lake sites, 21 river sites, and 1 pond. All samples were analyzed for nutrients; some samples were also analyzed for chlorophyll, phycocyanin, microcystin, and (or) phytoplankton community composition and abundance. Results from this study provide perspective for understanding the potential role nutrient and algal conditions have in facilitating the formation of HABs and may inform future actions to prevent and mitigate HABs and their potential effects on human and environmental health. In 2017, one low-flow floating synoptic on the Republican River into Zone C of Milford Lake and one 24-hour synoptic in Zone C of Milford Lake were completed. Results from the low-flow floating synoptic on July 17, 2017, at 21 river sites, 8 lake sites, and 1 pond site indicated that the Republican River was not contributing dissolved orthophosphate or total phosphorus concentrations higher than those in the main body of Milford Lake. No patterns in nutrient or total microcystin concentrations were evident from the 24-hour synoptic at two sites on August 24–25, 2017. Total nitrogen was dominated by total Kjeldahl nitrogen (TKN) at both sites. Different oscillation activity in algal biomass and chlorophyll at the two sites demonstrated the variable nature of algal accumulations and their effects on nutrient and dissolved oxygen concentrations. Different patterns in chlorophyll and microcystin concentrations indicate that the relation between algal biomass and cyanotoxin concentrations were different at the two sites, possibly because of differences among algal communities present at each site. Three whole-lake synoptics through Zones A, B, and C in Milford Lake were completed on July 10, August 9, and October 16–17, 2018, at 30 lake sites. Orthophosphate was consistently at least 77 percent of total phosphorus at all sites except the two most uplake sites. At the two most uplake sites, orthophosphate was between 52 and 72 percent of the total phosphorus present at the site. Concentrations of TKN were not consistently increasing or decreasing during 2018. Total nitrogen was dominated by TKN in July and August. Very low concentrations of dissolved nitrate plus nitrite indicate that the nutrient was likely tied up in algal biomass. By October, total nitrogen was approximately one-half TKN and one-half dissolved nitrate plus nitrite. Higher concentrations of dissolved orthophosphate and dissolved nitrate plus nitrite in October than in July and August were likely caused by reduced biological activity (less uptake of nutrients) and lower air and water temperatures. Multiple inflow events (streamflow greater than median daily value) between August and October also may have moved nutrients through the lake. Chlorophyll, phycocyanin, microcystin, and phytoplankton samples were collected at eight sites in 2018. Most sites had their highest chlorophyll concentrations in August. The three most uplake sites had their highest phycocyanin concentrations in July, whereas the other five sites had their highest phycocyanin concentrations in August. Two of 23 samples had detections of total microcystin (0.11 and 0.12 microgram per liter). Phytoplankton community composition mainly consisted of Bacillariophyta, Chlorophyta, Cryptophyta, and Cyanobacteria. Phytoplankton community composition and abundance data described broad seasonal patterns and did not capture the full range of possible conditions at each site.

Kansas

National Park Service Vegetation Mapping Inventory Program: Great Smoky Mountains National Park vegetation mapping project

The National Park Service (NPS) Vegetation Mapping Inventory (VMI) Program is an effort to classify, describe, and map existing vegetation communities in national park units throughout the United States. The NPS VMI Program is managed by the NPS Natural Resource Stewardship and Science Inventory and Monitoring Program and provides baseline vegetation information to natural resource managers, researchers, and ecologists. The U.S. Geological Survey Upper Midwest Environmental Sciences Center, NatureServe, and NPS Great Smoky Mountains National Park (GRSM, also referred to as the “Park”) have completed vegetation classification and mapping of GRSM, including the Foothills Parkway, for the NPS VMI Program. Mappers, ecologists, and botanists collaborated to affirm vegetation types of GRSM and to determine how best to map the vegetation types by using aerial imagery. A vegetation classification developed in 2003 by NatureServe and the NPS served as a foundation to further classify and map the vegetation types of the Park. Data from an additional 10 vegetation plots supported vegetation types either rare or not documented in the 2003 classification. Data from 203 verification sites were collected to test the field key to vegetation types and the application of vegetation types to a sample set of map polygons. Furthermore, data from 972 accuracy assessment (AA) sites were collected (of which 966 were used to test accuracy of the vegetation map layer). This GRSM vegetation mapping project identified 112 vegetation types consisting of 105 association types in the U.S. National Vegetation Classification (USNVC), 2 “park-special” types, 1 “map-special” type, and 4 cultural types in the USNVC. To map the vegetation and land cover of GRSM, 52 map classes were developed. Of these 52 map classes, 46 represent natural (including ruderal) vegetation types, most of which types are recognized in the USNVC. For the remaining 6 of the 52 map classes, 4 represent USNVC cultural types for agricultural and developed areas, and 2 represent non-USNVC types for nonvegetated open water and nonvegetated rock. Features were interpreted from viewing four-band digital aerial imagery using digital onscreen three-dimensional stereoscopic workflow systems in geographic information systems; digital aerial imagery was collected during September 23–October 30, 2015. The interpreted data were digitally and spatially referenced, thus making the spatial-database layers usable in a geographic information system. Polygon units were mapped to either a 0.5- or 0.25- hectare (ha) minimum mapping unit, depending on vegetation type. A geodatabase containing several feature-class layers and tables provides the locations and data of USNVC vegetation types (vegetation map layer), vegetation plots, verification sites, AA sites, project boundary extent, and aerial image centers and flight lines. Covering 210,875 ha, the feature-class layer and related tables for the vegetation map layer provide 34,084 polygons of detailed attribute data when special modifiers are not considered (average polygon size of 6.2 ha) and 36,589 polygons of detailed attribute data when special modifiers are considered (average polygon size of 5.8 ha). Each map polygon is assigned a map-class code and name and, when applicable, are linked to USNVC classification tables within the geodatabase. The vegetation map extent includes the administrative boundary for GRSM and the Foothills Parkway. A summary report, generated from the vegetation map layer, concludes that the 46 map classes representing natural (including ruderal) vegetation types apply to 99.2% of polygons (33,797 polygons; average size of 6.2 ha) and cover 98.6% of the Park (207,971.4 ha). Further broken down, map classes representing natural vegetation types indicate that the Park is 97.7% forest and woodland (205,882.5 ha), 0.6% shrubland (1,174.6 ha), and 0.4% herbaceous (914.3 ha). Map classes representing cultural vegetation types apply to 0.8% of polygons (259 polygons; average size of 4.9 ha) and cover 0.6% of the Park (1,277.4 ha). Map classes representing nonvegetation open and flowing water and unvegetated rock apply to 0.08% of polygons (28 polygons; average size of 58.1 ha) and cover 0.8% of the Park (1,625.9 ha). A thematic AA study was completed of map classes representing the natural (including ruderal) vegetation types of the Park. Initial AA results were discussed with NPS staff from the Park. Following input from NPS staff on how to handle map classes that fell below accuracy standards, adjustments were made to the vegetation map layer. Final results indicate an overall accuracy of 80.64% (kappa index of 79.96% for chance agreements) based on data from 966 of the 972 AA sites. Most individual map-class themes exceed the NPS VMI Program standard of 80% with a 90% confidence interval. The GRSM vegetation mapping project delivers many geospatial and vegetation data products, including an in-depth project report discussing methods and results, which includes map classification and map-class descriptions. This suite of products also includes descriptions and a field key to vegetation types; a database of vegetation plots, verification sites, and AA sites; digital images of field sites; field data sheets; digital aerial imagery; hardcopy and digital maps; a geodatabase of vegetation and land cover (map layer), field sites (vegetation plots, verification sites, and AA sites), aerial imagery index, project boundary, and metadata; and a contingency table listing AA results. Geospatial products are projected in the Universal Transverse Mercator, Zone 17 North, by using the North American Datum of 1983. Information on the NPS VMI Program and completed mapping projects are on the internet at https://www.nps.gov/im/vegetation-inventory.htm.

North Carolina, Tennessee

Capturing spatiotemporal patterns in presence-absence data to inform monitoring and sampling designs for the threatened Dakota skipper (Lepidoptera: Hesperiidae) in the Great Plains of the United States

Declines among species of insect pollinators, especially butterflies, has garnered attention from scientists and managers. Often these declines have spurred governments to declare some species as threatened or endangered. We used existing presence–absence data from surveys for the threatened Dakota skipper Hesperia dacotae (Skinner) to build statistical maps of species presence that could be used to inform future monitoring designs. We developed a hierarchical Bayesian modeling approach to estimate the spatial distribution and temporal trend in Dakota skipper probability of presence. Our model included a spatial random effect and fixed effects for the proportion of two grassland habitat types: those on well-drained soils and those on poorly drained soils; as well as the topographic slope. The results from this model were then used to assess sampling strategies with two different monitoring objectives: locating new Dakota skipper colonies or monitoring the proportion of historically (pre-2000) extant colonies. Our modeling results suggested that the distribution of Dakota skippers followed the distribution of remnant grasslands and that probabilities of presence tended to be higher in topographically diverse grasslands with well-drained soils. Our analysis also showed that the probability of presence declined throughout the northern Great Plains range. Our simulations of the different sampling designs suggested that new detections were expected when sampling where Dakota skippers likely occurred historically, but this may lead to a tradeoff with monitoring existing sites. Prior information about the extant sites may help to ameliorate this tradeoff.

Iowa, Minnesota, North Dakota, South Dakota

Population trends of smallmouth bass in the upper Colorado River basin with an evaluation of removal effects

Smallmouth bass Micropterus dolomieu were rare in the upper Colorado River basin until the early 1990’s when their abundance dramatically increased in the Yampa River sub-basin. Increased abundance was due primarily to colonization from Elkhead Reservoir, which was rapidly drawn down twice, first to make improvements to the dam (1992) and a second time for reservoir expansion (2005), and allowed escapement of resident bass to the river through an unscreened outlet. Elkhead Reservoir is located on Elkhead Creek, a tributary of the Yampa River. The rapid Elkhead Reservoir drawdown in 1992 was followed by a period of drought years with low, early runoff in the Yampa River sub-basin that benefitted smallmouth bass reproduction. This combination of factors allowed smallmouth bass to establish a self-sustaining population in the Yampa River. Subsequently, successful recruitment allowed smallmouth bass to disperse upstream and downstream in the Yampa River and eventually move into the downstream Green River. Smallmouth bass were also likely introduced, by unknown means, into the upper Colorado River and have since dispersed in this sub-basin. The rapid increase of smallmouth bass in the upper Colorado River basin overlapped with significant reductions in native fish populations in some locations. The threat to these native fishes initiated intensive mechanical removal of smallmouth bass by the Upper Colorado River Endangered Fish Recovery Program. In general, three factors explain fluctuating patterns in smallmouth bass density in the upper Colorado River basin in the last decade: reductions due to electrofishing removal, bass recovery after exploitation due to recruitment and immigration, and changes due to environmental factors not related to electrofishing and other management actions. Our analyses indicated that smallmouth bass densities were substantially reduced in most years by 7 electrofishing removal efforts. Less often, but dramatically in some cases, environmental effects were also responsible for significant declines in smallmouth bass densities in some reaches. Abundant year classes of young smallmouth bass produced in low flow and warm years such as 2007 have potential to overwhelm removal efforts, and the year class persists for one or more years. Nonetheless, it appears that increased electrofishing removal efforts from 2007 to 2011 resulted in sustained reductions in density of smallmouth bass sub-adults and adults throughout the upper basin despite environmental conditions that favored smallmouth bass reproduction in some years (e.g. 2007 and 2009), subsequent recruitment into sub-adult and adult age classes, and movement of smallmouth bass which previously (prior to increases in electrofishing removal efforts) allowed densities to recover in some reaches. We recommend that removal efforts continue in most areas of the upper basin but that the Recovery Program consider allocating effort based on population trends and suspected areas of highest smallmouth bass reproduction. For instance, reproduction, recruitment, and movement of smallmouth bass allowed densities to recover in some reaches, particularly Little Yampa Canyon. Smallmouth bass population recovery implies that areas such as Little Yampa Canyon itself or adjacent reaches (especially upstream), may provide important habitat for age-0 production. We recommend continued assessment of smallmouth bass populations in reaches where reproduction or age-1 nurseries are suspected, such as Little Yampa Canyon and the adjacent upstream reach. It may also be necessary to expand monitoring to areas surrounding suspected sources of smallmouth bass reproduction and increase electrofishing removal effort in these reaches.

Colorado, Utah

Landscape-scale variation in canopy water content of giant sequoias during drought

Recent drought (2012–2016) caused unprecedented foliage dieback in giant sequoias ( Sequoiadendron giganteum ), a species endemic to the western slope of the southern Sierra Nevada in central California. As part of an effort to understand and map sequoia response to droughts, we studied the patterns of remotely sensed canopy water content (CWC), both within and among sequoia groves in two successive years during the drought period (2015 and 2016). Our aims were: (1) to quantify giant sequoia responses to severe drought stress at a landscape scale using CWC as an indicator of crown foliage status, and (2) to estimate the effect of environmental correlates that mediate CWC change within and among giant sequoia groves. We utilized airborne high fidelity imaging spectroscopy (HiFIS) and light detection and ranging (LiDAR) data from the Carnegie Airborne Observatory to assess giant sequoia foliage status during 2015 and 2016 of the 2012–2016 droughts. A series of statistical models were generated to classify giant sequoias and to map their location in Sequoia and Kings Canyon National Parks (SEKI) and vicinity. We explored the environmental correlates and the spatial patterns of CWC change at the landscape scale. The mapped CWC was highly variable throughout the landscape during the two observation years, and proved to be most closely related to geological substrates, topography, and site-specific water balance. While there was an overall net gain in sequoia CWC between 2015 and 2016, certain locations (lower elevations, steeper slopes, areas more distant from surface water sources, and areas with greater climate water deficit) showed CWC losses. In addition, we found greater CWC loss in shorter sequoias and those growing in areas with lower sequoia stem densities. Our results suggest that CWC change indicates sequoia response to droughts across landscapes. Long-term monitoring of giant sequoia CWC will likely be useful for modeling and predicting their population-level response to future climate change.

California

Quantifying landscape-level biodiversity change in an island ecosystem: A 50-year assessment of shifts in the Hawaiian avian community

Hawaii has experienced profound declines in native avifauna alongside the introduction of numerous bird species. While site-specific population studies are common, landscape-level analyses of avian population dynamics are rare, particularly in island ecosystems. To address this gap, we used a density surface model to create a spatio-temporal projection of population densities and distributions across the Island of Hawai‘i, spanning nearly five decades (1976–2023). We incorporated environmental covariates of habitat, precipitation, and elevation, to further refine our projections. Our analysis encompassed nine native and six non-native bird species, inhabiting a range of ecological niches. We found five out of nine native species have declined in density and range size while four were stable. For non-native species, two were stable, one was decreasing, and three were increasing in density and range size. Our landscape projections can inform management by suggesting areas critical for habitat preservation and land acquisition for conservation, identifying where range fragmentation is occurring, and pinpointing locations of multi-species declines that are likely driven by a common cause. Our study demonstrates how long-term, landscape-level monitoring and analyses can advance understanding and addressing biodiversity loss, particularly in vulnerable tropical island ecosystems.

Hawaii

A geographic information system tool for aquatic resource conservation in the Red and Sabine River Watersheds of the southeast United States

Our goal was to build a geographic information system (GIS) tool to enhance modeling and hypothesis testing relevant to watersheds and fish fauna of the Red and Sabine Rivers in the southeastern United States. Species of concern were identified from wildlife action plans and Web sites. Spatial distributions of fish species and mercury in fillets were delineated using data from states. Public georeferenced data were obtained on land cover, soil type, forest canopy, impervious surfaces, wastewater facilities and 303(d) impaired waters. Overlay maps highlighted patterns across 8-digit hydrologic unit codes (HUCs). Bossier City, Louisiana and Beaumont, Texas areas displayed impervious surfaces over 10% and 303(d) waters per HUC were 20% and 8%, respectively. Because bowfin (Amia calva) (n=299) and bass (Micropterus spp.) (n=1493) occurred in up to 44% of HUCs and fillets contained elemental mercury concentrations across ranges monitored, they were appropriate indicators of bioavailable mercury. Of the total fish number showing >0.5ppm, 81% of records were derived from bowfin and bass, and stepwise multiple linear regressions indicated fish with mercury at these concentrations correlated with environmental variables. Detrended correspondence analysis showed total species occurrence and environmental relationships significant, where 81.6% of the variability in fish occurrence was explained by impervious surface, land cover other than canopy or impervious surface (such as wetlands and agricultural area) and canopy (forest type). Two-way indicator species analysis delineated species co-occurrence in HUCs (14 groups) and similarity of species composition (nine groups). Results identified three HUC groupings as potential targets for managerial interest. Quality control concerns for GIS development included site name data and priority rankings of critical fish species. This tool can be used to support modeling and trend analyses for several purposes, such as those relevant for developing and reporting on water quality standards and critical habitat assessments.

Arkansas, Louisiana, Oklahoma, Texas

Experimental studies in natural groundwater recharge dynamics: Assessment of recent advances in instrumentation

To quantify and model the natural groundwater-recharge process, two sites in south-central Kansas, U.S.A., were instrumented with various modern sensors and data microloggers. The atmospheric-boundary layer and the unsaturated and saturated soil zones were monitored as a unified regime. Data from the various sensors were collected using microloggers in combination with magnetic-cassette tape, graphical and digital recorders, analog paper-tape recorders, and direct observations to evaluate and automate data collection and processing. Atmospheric sensors included an anemometer, a tipping-bucket raingage, an air-temperature thermistor, a relative-humidity probe, a net radiometer, and a barometric-pressure transducer. Sensors in the unsaturated zone consisted of soil-temperature thermocouples, tensiometers coupled with pressure transducers and dial gages, gypsum blocks, and a neutron moisture probe operated by an observer. The saturated-zone sensors consisted of a water-level pressure transducer, a conventional float gage connected to a variable potentiometer, soil thermocouples, and a number of multiple-depth piezometers. Evaluation of the operation of these sensors and recorders indicated that certain types of equipment such as pressure transducers are very sensitive to environmental conditions. Extraordinary steps had to be taken to protect some of the equipment, whereas other equipment seemed to be reliable under all conditions. Based on such experiences, a number of suggestions aimed at improving such investigations are outlined.

Kansas

Workshop 3.5: Closing the gap between exposure and effects in monitoring studies

A major challenge to contaminant monitoring programs is the selection of an appropriate suite of measurements for assessing exposure and effects. Early monitoring programs relied solely on residue analysis to detect the organochlorine compounds that were in use at that time. A shift to the use of more transient, less persistent chemicals required that a new set of tools be developed to determine if an organism had been exposed. This led to the development of cellular and biochemical assays that could indicate the presence of these types of chemicals in biota and the environment. However, it was recognized that measures of contaminant presence alone were insufficient to assess the health of biota. As a result, considerable research began to be directed toward development of diagnostic tools for measuring chemical effects in fish and wildlife. Today, contaminant monitoring programs follow a paradigm for study design that emphasizes not only the use of measures of exposure, but also measures of effect. Using data from our monitoring and research studies for hormonally active substances, we discuss a variety of metrics of exposure and effects and their application to specific chemicals, and the current information gaps. We conclude that although several bioindicators of exposure and effect have been promoted and used, to date there continues to be a poor association between cause and effect for endocrine active substances. In part, this is due to the limited number of diagnostic tools that are available and to a lack of basic toxicological information concerning toxicokinetics and mechanisms of action of hormonally active chemicals in fish and wildlife species. In the foreseeable future, both tissue and environmental residue data, despite the many limitations, will continue to be an important component of monitoring programs for hormonally active chemicals as we continue to develop and validate more specific bioindicators of exposure and effects.

Pure and Applied Chemistry

ERF1_2 -- Enhanced River Reach File 2.0

The digital segmented network based on watershed boundaries, ERF1_2, includes enhancements to the U.S. Environmental Protection Agency's River Reach File 1 (RF1) (USEPA, 1996; DeWald and others, 1985) to support national and regional-scale surface water-quality modeling. Alexander and others (1999) developed ERF1, which assessed the hydrologic integrity of the digital reach traces and calculated the mean water time-of-travel in river reaches and reservoirs. ERF1_2 serves as the foundation for SPARROW (Spatially Referenced Regressions (of nutrient transport) on Watershed) modeling. Within the context of a Geographic Information System, SPARROW estimates the proportion of watersheds in the conterminous U.S. with outflow concentrations of several nutrients, including total nitrogen and total phosphorus, (Smith, R.A., Schwarz, G.E., and Alexander, R.B., 1997). This version of the network expands on ERF1 (Version 1.2; Alexander, et al., 1999) and includes the incremental and total drainage area derived from 1-kilometer (km) elevation data for North America. Previous estimates of the water time-of-travel were recomputed for reaches with water-quality monitoring sites that included two reaches. The mean flow and velocity estimates for these split reaches are based on previous estimation methods (Alexander et al., 1999) and are unchanged in ERF1_2. Drainage area calculations provide data used to estimate the contribution of a given nutrient to the outflow. Data estimates depend on the accuracy of node connectivity. Reaches split at water-quality or pesticide-monitoring sites indicate the source point for estimating the contribution and transport of nutrients and their loads throughout the watersheds. The ERF1_2 coverage extends the earlier drainage area founded on the 1-kilometer data for North America (Verdin, 1996; Verdin and Jenson, 1996). A 1-kilometer raster grid of ERF1_2 projected to Lambert Azimuthal Equal Area, NAD 27 Datum (Snyder, 1987), was merged with the HYDRO1K flow direction data set (Verdin and Jenson, 1996) to generate a DEM-based watershed grid, ERF1_2WS_LG. The watershed boundaries are maintained in a raster (grid cell) format as well as a vector (polygon) format for subsequent model analysis. Both the coverage, ERF1_2, and the grid, ERF1_2WS_LG, are available at: URL:http://water.usgs.gov/lookup/getspatial?erf1_2

Open-File Report

Integrative monitoring strategy for marine and freshwater harmful algal blooms and toxins across the freshwater-to-marine continuum

Many coastal states throughout the USA have observed negative effects in marine and estuarine environments caused by cyanotoxins produced in inland waterbodies that were transported downstream or produced in the estuaries. Estuaries and other downstream receiving waters now face the dual risk of impacts from harmful algal blooms (HABs) that occur in the coastal ocean as well as those originating in inland watersheds. Despite this risk, most HAB monitoring efforts do not account for hydrological connections in their monitoring strategies and designs. Monitoring efforts in California have revealed the persistent detection of cyanotoxins across the freshwater-to-marine continuum. These studies underscore the importance of inland waters as conduits for the transfer of cyanotoxins to the marine environment and highlight the importance of approaches that can monitor across hydrologically connected waterbodies. A HAB monitoring strategy is presented for the freshwater-to-marine continuum to inform HAB management and mitigation efforts and address the physical and hydrologic challenges encountered when monitoring in these systems. Three main recommendations are presented based on published studies, new datasets, and existing monitoring programs. First, HAB monitoring would benefit from coordinated and cohesive efforts across hydrologically interconnected waterbodies and across organizational and political boundaries and jurisdictions. Second, a combination of sampling modalities would provide the most effective monitoring for HAB toxin dynamics and transport across hydrologically connected waterbodies, from headwater sources to downstream receiving waterbodies. Third, routine monitoring is needed for toxin mixtures at the land–sea interface including algal toxins of marine origins as well as cyanotoxins that are sourced from inland freshwater or produced in estuaries. Case studies from California are presented to illustrate the implementation of these recommendations, but these recommendations can also be applied to inland states or regions where the downstream receiving waterbody is a freshwater lake, reservoir, or river.

California, Oregon

A new method for bioassessment of ecosystems with complex communities and environmental gradients

Bioassessment of complex and heterogeneous ecosystems is a challenge when there are multiple, strong, natural environmental gradients; unknown, or spatially varying, mixtures of stressors; and large numbers of taxa with unknown responses to both the environmental gradients and the stressors. Current methods of bioassessment are not designed for use under this set of constraints. To address this gap, we have developed an assessment method appropriate for well-sampled, heterogeneous systems with many taxa. In the bioassessment described below, we model taxa occurrence as a function of natural environmental gradients, then use residual covariance patterns between all pairs of taxa to estimate the impact of human disturbance across sites as a latent construct. The derivation of the method from an underlying causal model allows the metric value at each site and the associated taxa responses to be partitioned into contributions from a set of putative stressors. We apply this method as a case study to the subtidal benthic invertebrate community of Puget Sound, WA (USA) and demonstrate a partial decomposition of the metric values to a set of stressors including sediment organic carbon, nitrogen, metals, and organic pollutants. While this method provides new opportunities to estimate, communicate, and understand the ecological condition of complex, heterogeneous ecosystems, due to the requirement for broad, detailed data to inform its estimates, it will likely be most appropriate for monitoring programs.

Washington

Bait stations, hard mast, and black bear population growth in Great Smoky Mountains National Park

Bait-station surveys are used by wildlife managers as an index to American black bear ( Ursus americanus ) population abundance, but the relationship is not well established. Hard mast surveys are similarly used to assess annual black bear food availability which may affect mortality and natality rates. We used data collected in Great Smoky Mountains National Park (GSMNP) from 1989 to 2003 to determine whether changes in the bait-station index (ΔBSI) were associated with estimated rates of bear population growth (λ) and whether hard mast production was related to bear visitation to baits. We also evaluated whether hard mast production from previous years was related to λ. Estimates of λ were based on analysis of capture-recapture data with the Pradel temporal symmetry estimator. Using the Akaike's Information Criterion (AIC), our analysis revealed no direct relationship between ΔBSI and λ. A simulation analysis indicated that our data were adequate to detect a relationship had one existed. Model fit was marginally improved when we added total oak mast production of the previous year as an interaction term suggesting that the BSI was confounded with environmental variables. Consequently the utility of the bait-station survey as a population monitoring technique is questionable at the spatial and temporal scales we studied. Mast survey data, however, were valuable covariates of λ. Population growth for a given year was negatively related to oak mast production 4 and 5 years prior. That finding supported our hypothesis that mast failures can trigger reproductive synchrony, which may not be evident from the trapped sample until years later.

North Carolina, Tennessee

Assessment of dissolved-selenium concentrations and loads in the Lower Gunnison River Basin, Colorado, as part of the Selenium Management Program, 2011–17

The Gunnison Basin Selenium Management Program implemented a water-quality monitoring network in 2011 to measure concentrations of selenium in the lower Gunnison River Basin in Colorado. Selenium is a trace element that bioaccumulates in aquatic food chains. Selenium is essential for life, but elevated amounts can cause reproductive failure, deformities, and other harmful effects. The primary goal of the Selenium Management Program is to meet the State of Colorado water-quality standard of 4.6 micrograms per liter (µg/L) for dissolved selenium at the U.S. Geological Survey (USGS) streamflow-gaging station number 09152500—Gunnison River near Grand Junction, Colorado—herein referred to as “Whitewater.” The U.S. Geological Survey, in cooperation with the Bureau of Reclamation, has completed a review of dissolved-selenium data collected from the Selenium Management Program network during Water Year (WY) 2017 (October 1, 2016 through September 30, 2017) to further the understanding of the status and trends of selenium in the basin. This report presents the percentile values for selenium because regulatory agencies in Colorado make decisions based on the U.S. Environmental Protection Agency’s Clean Water Act section 303(d), which uses percentile values for concentrations. Also presented are dissolved-selenium loads at 14 sites in the lower Gunnison River Basin for WYs 2011–17. Annual dissolved-selenium loads were calculated for six sites with continuous U.S. Geological Survey streamflow-gaging stations. These six sites are referred to as “core” sites in this report. The remaining sites, which do not have streamflow-gaging stations, are referred to as “ancillary” sites in this report. During WY 2017, the loads calculated at the six core sites ranged from 306 pounds (lb) at Uncompahgre River at Colona to 12,600 lb at Whitewater, respectively. By using discrete water-quality samples and the associated discharge measurements, instantaneous loads were calculated for 14 sites in WYs 2011–17 where discrete water-quality sampling took place. Median instantaneous loads ranged from 0.52 pounds per day (lb/d) at Uncompahgre River at Colona to 35.7 lb/d at Whitewater. Mean instantaneous loads ranged from 0.63 lb/d at Cummings Gulch at mouth to 35.5 lb/d at Whitewater. Most tributary sites in the basin had a median instantaneous dissolved-selenium load of less than 20.0 lb/d. In general, dissolved-selenium loads at Gunnison River main-stem sites showed an increase from upstream to downstream. The State of Colorado’s water-quality standard for dissolved selenium of 4.6 µg/L was compared to the 85th percentiles for dissolved selenium at selected sites. Annual 85th percentiles for dissolved selenium were calculated by using estimated dissolved-selenium concentrations from linear regression models for the six core sites with U.S. Geological Survey streamflow-gaging stations. The 85th-percentile concentrations for WY 2017 based on this method ranged from 0.68 µg/L at Uncompahgre River at Colona to 140 µg/L at Loutzenhizer Arroyo at North River Road. The 85th percentiles for concentrations of dissolved selenium also were calculated from water-quality samples collected during WY 2017 from sites with sufficient data. The annual 85th-percentile concentrations based on the discrete samples ranged from 0.75 µg/L at Uncompahgre River at Colona to 106 µg/L at Loutzenhizer Arroyo at North River Road. An analysis was completed for Whitewater to determine if an upward or downward trend exists for dissolved-selenium loads during two time periods. The first time period included all data at Whitewater, whereas the second time period focused on more recent data. The trend analysis indicates a decrease from 22,200 to 12,600 lb, which is a 43.1 percent (9,600 lb) reduction during the time period WY 1986 through WY 2017. The trend analysis for the annual dissolved-selenium load for WY 1995 through WY 2017 indicates a decrease of 6,600 lb per year, or 35.5 percent. An evaluation of laboratory bias was completed for selenium data which was used in the trend analysis. Findings indicated a potential positive bias of approximately 12 percent may exist in the data from October 2005 through August 2015.

Colorado

Ground-water vulnerability to nitrate contamination in the mid-atlantic region

The U.S. Environmental Protection Agency?s (USEPA) Regional Vulnerability Assessment (ReVA) Program has developed a set of statistical tools to support regional-scale, integrated ecological risk-assessment studies. One of these tools, developed by the U.S. Geological Survey (USGS), is used with available water-quality data obtained from USGS National Water-Quality Assessment (NAWQA) and other studies in association with land cover, geology, soils, and other geographic data to develop logistic-regression equations that predict the vulnerability of ground water to nitrate concentrations exceeding specified thresholds in the Mid-Atlantic Region. The models were developed and applied to produce spatial probability maps showing the likelihood of elevated concentrations of nitrate in the region. These maps can be used to identify areas that currently are at risk and help identify areas where ground water has been affected by human activities. This information can be used by regional and local water managers to protect water supplies and identify land-use planning solutions and monitoring programs in these vulnerable areas.

Fact Sheet

State of the Earth’s cryosphere at the beginning of the 21st century: Glaciers, global snow cover, floating ice, and permafrost and periglacial environments

This chapter is the tenth in a series of 11 book-length chapters, collectively referred to as “this volume,” in the series U.S. Geological Survey Professional Paper 1386, Satellite Image Atlas of Glaciers of the World. In the other 10 chapters, each of which concerns a specific glacierized region of Earth, the authors used remotely sensed images, primarily from the Landsat 1, 2, and 3 series of spacecraft, in order to analyze that glacierized region and to monitor changes in its glaciers. Landsat images, acquired primarily during the period 1972 through 1981, were used by an international team of glaciologists and other scientists to study the various glacierized regions and (or) to discuss related glaciological topics. In each glacierized region, the present distribution of glaciers within its geographic area is compared, wherever possible, with historical information about their past areal extent. The atlas provides an accurate regional inventory of the areal extent of glacier ice on our planet during the 1970s as part of an expanding international scientific effort to measure global environmental change on the Earth’s surface. However, this chapter differs from the other 10 in its discussion of observed changes in all four elements of the Earth’s cryosphere (glaciers, snow cover, floating ice, and permafrost) in the context of documented changes in all components of the Earth System. Human impact on the planet at the beginning of the 21st century is pervasive. The focus of Chapter A is on changes in the cryosphere and the importance of long-term monitoring by a variety of sensors carried on Earth-orbiting satellites or by a ground-based network of observatories in the case of permafrost. The chapter consists of five parts. The first part provides an introduction to the Earth System, including the interrelationships of the geosphere (cryosphere, hydrosphere, lithosphere, and atmosphere), the biosphere, climate processes, biogeochemical cycles, and the critically important hydrologic cycle, in which glacier ice is the second largest reservoir of water after the oceans. The second part assesses the state of glaciers in all of the glacierized regions of the planet, primarily as drawn in the other 10 chapters. It includes sections on ice cores and the climate record they contain, volumetric changes in glaciers, harnessing spaceborne sensors to measure changes in glaciers, and related topics. The third part summarizes trends in global snow cover. The fourth part summarizes long-term changes in area and thickness of floating ice, including polar sea ice and freshwater (lake and river) ice. The fifth part assesses the loss of permafrost and changes in periglacial environments at high latitudes and high altitudes.

Professional Paper