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Accuracy assessment of NLCD 2006 land cover and impervious surface

Release of NLCD 2006 provides the first wall-to-wall land-cover change database for the conterminous United States from Landsat Thematic Mapper (TM) data. Accuracy assessment of NLCD 2006 focused on four primary products: 2001 land cover, 2006 land cover, land-cover change between 2001 and 2006, and impervious surface change between 2001 and 2006. The accuracy assessment was conducted by selecting a stratified random sample of pixels with the reference classification interpreted from multi-temporal high resolution digital imagery. The NLCD Level II (16 classes) overall accuracies for the 2001 and 2006 land cover were 79% and 78%, respectively, with Level II user's accuracies exceeding 80% for water, high density urban, all upland forest classes, shrubland, and cropland for both dates. Level I (8 classes) accuracies were 85% for NLCD 2001 and 84% for NLCD 2006. The high overall and user's accuracies for the individual dates translated into high user's accuracies for the 2001–2006 change reporting themes water gain and loss, forest loss, urban gain, and the no-change reporting themes for water, urban, forest, and agriculture. The main factor limiting higher accuracies for the change reporting themes appeared to be difficulty in distinguishing the context of grass. We discuss the need for more research on land-cover change accuracy assessment.

Remote Sensing of Environment

Deserts of China

Improving arid land quality requires an understanding of the original state of the land and its relationship to wind, water, and plant regimes, as well as understanding of interactions within the present ecosystem. Chinese scientists and local residents have made significant advances in improving arid environments in gobi and sandy deserts and in less arid sandy lands. Wind patterns are being changed by planting forest belts to protect oases and sandy lands, and on a smaller scale by planting grasses and shrubs or constructing straw grids. Research on reclamation of deserts is now focusing on how sand-fixing plants may be adapted to local environments, and how the resources of grazing land and water may be effectively exploited without being overused.

American Scientist

Ecosystem vs. community recovery 25 years after grass invasions and fire in a subtropical woodland

Despite a large body of research documenting invasive plant impacts, few studies have followed individual invaded sites over decades to observe how they change, and none have contrasted how compositional impacts from invasion compare to ecosystem-process impacts over a multi-decadal time-scale. Using direct measurements of plant density and composition and of ecosystems processes, we evaluate how ecosystem structure, above-ground net primary production (ANPP), and above-ground and soil nutrient pools compare over 25 years since fire and C4 grass invasions disrupted seasonally dry Hawaiian woodlands. We compare structure and function between primary woodland that has never burned and is largely native species-dominated, with sites that had been the same woodland type but burned in alien-grass-fuelled fires in the 1970s and 1980s. The sites have not experienced fires since 1987. We report here that woody plant composition and structure continue to be dramatically changed by the initial invasions and fires that occurred 25 years ago and invaders continue to dominate in burned sites. This is reflected in continued low plant carbon pools in burned compared to unburned sites. Yet ANPP and N storage, which were dramatically lower in the initial decade after invasive-grass fuelled fires, have increased and are now indistinguishable from values measured in intact woodlands. Soil carbon pools were resilient to both invasion and fire initially and over time. Above-ground net primary production has recovered because of invasion of burned sites by a non-native N-fixing tree rather than because of recovery of native species. This invasive N-fixing tree is unlikely to return C storage of the invaded sites to those of unburned woodland because of its tissue and growth characteristics and its interactions with invasive grasses. It does not facilitate native species but rather promotes a persistent invasive grass/N-fixer savanna. Synthesis. We conclude that fire, an unusual disturbance in this system, has perpetuated the dominance of these sites by invasive species and that despite the dramatic recovery of above-ground net primary production and N pools, the ecosystem continues to be in a distinctly different state than the pre-fire, pre-Melinis community. Thus, despite the absence of further disturbance (fire), there is no evidence that succession towards the original ecosystem is occurring. The fact that N pools and above-ground net primary production recover because of a new invader (Morella faya), highlights the unpredictability of ecosystem trajectories in the face of altered regional species pools.

Hawaii

Land uses, fire, and invasion: Exotic annual Bromus and human dimensions

Human land uses are the primary cause of the introduction and spread of exotic annual Bromus species. Initial introductions were likely linked to contaminated seeds used by homesteading farmers in the late 1880s and early 1900s. Transportation routes aided their spread. Unrestricted livestock grazing from the 1800s through the mid-1900s reduced native plant competitors leaving large areas vulnerable to Bromus dominance. Ecosystems with cooler and moister soils tend to have greater potential to recover from disturbances (resilience) and to be more resistant to Bromus invasion and dominance. Warmer and drier ecosystems are less resistant to Bromus and are threatened by altered fire regimes which can lead to Bromus dominance, impacts to wildlife, and alternative stable states. Native Americans used fire for manipulating plant communities and may have contributed to the early dominance of Bromus in portions of California. Fire as a tool is now limited to site preparation for revegetation in most ecosystems where Bromus is a significant problem. Once Bromus dominates, breaking annual grass/fire cycles requires restoring fire-tolerant perennial grasses and forbs, which can compete with Bromus and resist its dominance. Current weed management policies often lack regulations to prevent further expansion of Bromus . Research is needed on how and where livestock grazing might help increase perennial grass and forb cover and density to create ecosystems that are more resistant to Bromus . Also, studies are needed to ascertain the role, if any, of oil and gas development in contributing to the spread of Bromus .

Book chapter

Assessing the probability of grass carp (Ctenopharyngodon idella) spawning in the Sandusky River using discharge and water temperature

Grass carp ( Ctenopharyngodon idella , Val.) is an invasive species in the Laurentian Great Lakes region with the potential for damaging the lake ecosystem and harming the region's economy. Grass carp spawning was documented in the Sandusky River, Ohio, in 2015 through targeted egg sampling. Continued egg sampling in the Sandusky River suggested that grass carp spawning is related to discharge and water temperature. We used egg sampling data from 2014 to 2021 to develop a Bayesian model to understand the likely conditions related to grass carp spawning in the Lake Erie watershed. The resulting model estimates the likelihood of spawning as a function of discharge and water temperature. The results suggest that spawning is most likely to occur when discharge is above 10 m 3 /s and water temperature is below 25 ℃. The model provides a tool for setting research and management priorities to develop management strategies to reduce the grass carp population in Lake Erie. Furthermore, the Bayesian nature of the model makes the model updatable when new data are available, whether from the same river or from another river, to incorporate river-specific features to identify likely spawning rivers.

Ohio

Born of fire - restoring sagebrush steppe

Fire is a natural feature of sagebrush grasslands in the Great Basin. The invasion of exotic annual grasses, such as Bromus tectorum (cheatgrass), has changed the environment in these ecosystems. Invasive annual grasses provide a dense and continuous source of fuel that extends the season for fires and increases the frequency of fires in the region. Frequent fires eventually eliminate the native sagebrush. These annual grasses also change soil nutrients, especially carbon and nitrogen, such that invasive annual grasses are favored over the native plants. The Forest and Rangeland Ecosystem Science Center of the U.S. Geological Survey (USGS) is studying how to reduce the problems caused by these invasive annual grasses and restore native sagebrush grasslands. The areas of research include understanding disturbance regimes, especially fire, discerning the role of nutrients in restoring native plants, determining the potential to restore forbs important for wildlife, and ascertaining the past and present use of native and nonnative plants in revegetation projects.

Fact Sheet

U.S. Geological Survey invasive carp strategic framework, 2023–27

U.S. Geological Survey (USGS) research has supported management of Hypophthalmichthys nobilis (bighead carp), Mylopharyngodon piceus (black carp), Ctenopharyngodon idella (grass carp), and H. molitrix (silver carp), hereafter referred to collectively as invasive carps, for over a decade. This strategic framework identifies thematic research areas to guide funding decisions for USGS invasive carp research during 2023–27. The USGS will use this framework and input from research and management agencies to identify leading-edge research to advance control of these highly damaging invasive species.

Circular

Effects of exotic grasses on soil seed banks in Southeastern Arizona grasslands

At the Appleton-Whittell Research Ranch, an ungrazed grassland preserve in southeastern Arizona, soil seed banks were sampled in June, August, and October 2002 and June 2003. Wildfire had previously burned 90% of the research ranch in May 2002. Seed density and species richness in burned native grassland (2 plots) were compared to those in burned exotic grassland (2 plots). Averaged over 4 sample dates, seed densities were as follows: burned native grassland, 591 ± 243.1 seeds ·m −2 and 784 ± 334.9 seeds ·m −2 ; burned exotic grassland, 501 ± 198.9 seeds ·m −2 and 196 ± 123.8 seeds ·m −2 . Species richness in the seed bank, also averaged over 4 sample dates, was as follows: burned native grassland, 16.3 ± 1.7 species ·m −2 and 19.5 ± 1.0 species ·m −2 ; burned exotic grassland, 12.0 ± 3.4 species ·m −2 and 11.06 ± 2.5 species ·m −2 . The seed bank of burned exotic grassland contained significantly fewer seeds and species than that of burned native grassland. In addition, the seed bank in burned exotic grassland comprised mainly exotic grasses, whereas annual and perennial herbs, most of them native, dominated the seed bank of burned native grassland. Of the 50 species detected in soil samples, only 20 had a persistent seed bank, and only 1 of these was a native perennial bunchgrass. The preponderance of transient species means that eradication of exotic grasses must be followed by reseeding of native grasses and herbs, perhaps repeatedly, if native grassland is to replace exotic grassland.

Arizona

Patterns of post-fire invasion of semiarid shrub-steppe reveals a diversity of invasion niches within an exotic annual grass community

Disturbances such as fire provide an opportunity for invasive plant species to exploit newly created niche space. Whether initial invaders facilitate, compete with, or do not affect later invaders is important to determine in communities affected by multiple invaders. This analysis focuses on the newer invaders Taeniatherum caput-medusae (medusahead) and Ventenata dubia (ventenata) in sagebrush-steppe communities previously invaded by Bromus tectorum (cheatgrass), during the first 5 years of recovery after wildfire . We combined probabilistic co-occurrence analysis and Getis-Ord spatial clustering analysis to assess relationships between different exotic annual grass species and native and introduced perennial bunchgrasses, then used Bayesian generalized linear models to determine if and how medusahead and ventenata differed in their environmental relationships and thus invasion niches. Medusahead presence was positively associated with both other exotic annual grasses, but ventenata presence was negatively associated with cheatgrass presence. Medusahead hotspots were more spatially similar to cheatgrass hotspots while ventenata hotspots were unique. Both invaders were negatively related to total perennial bunchgrass cover but disassociations between invaders and different perennial bunchgrasses were species-specific. Medusahead and ventenata occupied different niches; medusahead in low elevation, low precipitation areas and ventenata in higher elevation, higher precipitation areas. Despite seemingly similar ecology and growth requirements among these annual grasses and a tendency to be considered uniformly in both research and management, the species appeared to have different invasion niches.

Idaho, Oregon

Feasibility of an implantable capsule for limiting lifespan of grass carp

The grass carp (Ctenopharyngodon idella) is an herbivorous cyprinid stocked to control undesirable aquatic vegetation. However, stocking grass carp presents several problems including complete eradication of submersed aquatic vegetation, dispersal out of the target area, adverse effects on fish communities, and damage to waterfowl habitat and native vegetation. The purpose of this research was to consider the feasibility of an implantable capsule for limiting the lifespan of grass carp. Stainless steel dowel pins were inserted into 49 fish to identify the most appropriate site to implant the capsule. The throat region along the body's longitudinal axis was identified as the most suitable location because it resulted in minimal loss over an 8-month holding period. Rotenone solutions were injected into the ventral surface between the pelvic fins to determine the lethal dosage to 95% of the population (LD 95). The LD95 for grass carp increased curvilin-early with fish weight. Four polymers that merit further evaluation in constructing the capsule are poly[bis(p-carboxyphenoxy) propane anhydride], poly[bis(p- carboxyphenoxy) hexane anhydride], poly-1-lactide, and poly(??-caprolactone) . Implants are commonly used to deliver pharmaceutical products in medical and veterinarian applications, and have been used in fish. Developing a bioerodible capsule could increase the safety and flexibility of stocking grass carp for control of aquatic plants, and may also be applicable for management of other exotic species.

Journal of Aquatic Plant Management

Despite regional variation, Gymnorhinus cyanocephalus (Pinyon Jay) densities generally increase with local pinyon–juniper cover and heterogeneous ground cover

Traditionally, local-scale habitat-relationship models are developed over small spatial extents, limiting model transferability and inference outside the study area. Thus, habitat managers frequently lack fine-scale information regarding the influence of vegetation composition and structure on site suitability or species abundance. Gymnorhinus cyanocephalus (Pinyon Jay) represents one declining species for which managers have limited information regarding the influence that vegetation composition and structure have on abundance at broad scales. To address this need, we developed a hierarchical Bayesian abundance model using summertime bird and vegetation data collected under the Integrated Monitoring in Bird Conservation Regions program to explain jay abundance as a function of local conditions. Our G. cyanocephalus abundance model allowed abundance relationships with pinyon pine ( Pinus edulis and P. monophylla ) and juniper ( Juniperus spp.) to vary by ecoregion, thereby accounting for potential regional differences in habitat associations. We found G. cyanocephalus abundance was generally positively associated with pinyon pine and juniper cover; however, habitat relationships varied by ecoregion. Additionally, we found positive associations between jay abundance and grass cover, sagebrush cover, and percent bare ground. Our results agree with prior research suggesting mechanical removal of pinyon pine and juniper trees for sagebrush restoration or fuel treatments may negatively affect G. cyanocephalus . Managers wishing to reduce pinyon and juniper tree cover without negatively affecting G. cyanocephalus may benefit from targeting sites where both large-scale distribution models and our local habitat relationships suggest G. cyanocephalus are likely to occur in low numbers. Additionally, our modeled relationships indicate restoration that increases grass cover, sagebrush cover, and bare ground, while maintaining pinyon and (or) juniper cover, may lead to increased local densities of G. cyanocephalus .

Ornithological Applications

Preliminary analytical results for ash and burned soils from the October 2007 southern California wildfires

The U.S. Geological Survey (USGS) collected ash and burned soils from about 28 sites in southern California wildfire areas (Harris, Witch, Ammo, Santiago, Canyon and Grass Valley) from Nov. 2 through 9, 2007 (table 1). USGS researchers are applying a wide variety of analytical methods to these samples, with the goal of helping identify characteristics of the ash and soils from wildland and suburban burned areas that may be of concern for their potential to adversely affect water quality, human health, endangered species, and debris-flow or flooding hazards. These studies are part of the Southern California Multi-Hazards Demonstration Project, and preliminary findings are presented here.

Open-File Report

U.S. Geological Survey Karst Interest Group Proceedings, Carlsbad, New Mexico, April 29-May 2, 2014

Karst aquifer systems are present throughout parts of the United States and some of its territories, and have developed in carbonate rocks (primarily limestone and dolomite) that span an interval of time encompassing more than 550 million years. The depositional environments, diagenetic processes, post-depositional tectonic events, and geochemical weathering processes that form karst aquifers are varied and complex, and involve biological, chemical, and physical changes. These factors, combined with the diverse climatic regimes under which karst development in these rocks has taken place, result in the unique dual- or triple-porosity nature of karst aquifers. These complex hydrogeologic systems typically represent challenging and unique conditions to scientists attempting to study groundwater flow and contaminant transport in these terrains. The dissolution of carbonate rocks and the subsequent development of distinct and beautiful landscapes, caverns, and springs has resulted in the most exceptional karst areas of the United States being designated as national or state parks; commercial caverns and known privately owned caves number in the tens of thousands. Both public and private properties provide access for scientists to study the flow of groundwater in situ. Likewise, the range and complexity of landforms and groundwater flow systems associated with karst terrains are enormous, perhaps more than for any other aquifer type. Karst aquifers and landscapes that form in tropical areas, such as the cockpit karst along the north coast of Puerto Rico, differ greatly from karst landforms in more arid climates, such as the Edwards Plateau in west-central Texas or the Guadalupe Mountains near Carlsbad, New Mexico, where hypogenic processes have played a major role in speleogenesis. Many of these public and private lands also contain unique flora and fauna associated with these karst hydrogeologic systems. As a result, numerous federal, state, and local agencies have a strong interest in the study of karst terrains. Many of the major springs and aquifers in the United States have developed in carbonate rocks, such as the Floridan aquifer system in Florida and parts of Alabama, Georgia, and South Carolina; the Ozark Plateaus aquifer system in parts of Arkansas, Kansas, Missouri, and Oklahoma; and the Edwards-Trinity aquifer system in west-central Texas. These aquifers, and the springs that discharge from them, serve as major water-supply sources and as unique ecological habitats. Competition for the water resources of karst aquifers is common, and urban development and the lack of attenuation of contaminants in karst areas can impact the ecosystem and water quality of these aquifers. The concept for developing a platform for interaction among scientists within the U.S. Geological Survey (USGS) working on karst-related studies evolved from the November 1999 National Ground-Water Meeting of the USGS. As a result, the Karst Interest Group (KIG) was formed in 2000. The KIG is a loose-knit, grass-roots organization of USGS and non-USGS scientists and researchers devoted to fostering better communication among scientists working on, or interested in, karst science. The primary mission of the KIG is to encourage and support interdisciplinary collaboration and technology transfer among scientists working in karst areas. Additionally, the KIG encourages collaborative studies between the different mission areas of the USGS as well as other federal and state agencies, and with researchers from academia and institutes. The KIG also encourages younger scientists by participation of students in the poster and oral sessions. To accomplish its mission, the KIG has organized a series of workshops that are held near nationally important karst areas. To date (2014) six KIG workshops, including the workshop documented in this report, have been held. The workshops typically include oral and poster sessions on selected karst-related topics and research, as well as field trips to local karst features. Proceedings of the workshops are published by the USGS and are available online at http://water.usgs.gov/ogw/karst/kig . The first KIG workshop was held in St. Petersburg, Florida, February 13–16, 2001, in the vicinity of the large springs and other karst features of the Floridan aquifer system. The second KIG workshop was held August 20–22, 2002, in Shepherdstown, West Virginia, in proximity to the carbonate aquifers of the northern Shenandoah Valley and highlighted an invited presentation on karst literature by the late Barry F. Beck of P.E. LaMoreaux and Associates. The third KIG workshop was held September 12–15, 2005, in Rapid City, South Dakota, nearby to karst features in evaporites and limestones of the Madison Group in the Black Hills of South Dakota, including Wind Cave National Park and Jewel Cave National Monument. The workshop also included a featured presentation by Thomas Casadevall, Central Region Director, USGS, on the status of earth science at the USGS and evening trips to Jewel Cave led by Mike Wiles, National Park Service (NPS) and Wind Cave led by Rod Horrocks, NPS. The fourth KIG workshop was held May 27–29, 2008, and hosted by the Hoffman Environmental Research Institute and Center for Cave and Karst Studies at Western Kentucky University in Bowling Green, Kentucky, near Mammoth Cave National Park and karst features of the Chester Upland and Pennyroyal Plateau. The workshop featured a late-night field trip into Mammoth Cave with Rickard Toomey and Rick Olsen, NPS. The fifth workshop was held April 26–29, 2011, and was a joint meeting of the USGS KIG and University of Arkansas HydroDays, hosted by the Department of Geosciences at the University of Arkansas in Fayetteville. The workshop featured an outstanding field trip to the unique karst terrain along the Buffalo National River of the southern Ozarks and a keynote presentation on paleokarst in the United States by Art and Peggy Palmer. This sixth and current 2014 KIG workshop is hosted by the National Cave and Karst Research Institute (NCKRI) in Carlsbad, New Mexico, with Director of NCKRI, George Veni, serving as co-chair of the workshop with Eve Kuniansky, USGS. The session planning committee for this sixth workshop includes Van Brahana, USGS retired and University of Arkansas Professor Emeritus; Tom Byl, USGS and Tennessee State University; Zelda Bailey, former Director of NCKRI and retired Director, National Institute of Standards and Technology, Boulder Laboratory, Colorado; Patrick Tucci, USGS retired; and Mike Bradley, Allan Clark, Geoff Delin, Daniel Doctor, James Kaufmann, Eve Kuniansky, Randy Orndorff, Larry Spangler, and Dave Weary of the USGS. The karst hydrology field trip on Thursday will be led by Lewis Land (NCKRI karst hydrologist) and the optional Friday field trip on the geology of Carlsbad Caverns National Park will be led by George Veni. The keynote speaker is Dr. Penelope Boston, Director of Cave and Karst Studies at New Mexico Tech, Socorro, and Academic Director at NCKRI, who will address the future of karst research. Additionally, there is a featured presentation “Irish karst and its management,” by Caoimhe Hickey, The Geological Survey of Ireland, preceding a panel discussion on “Collaboration During Times of Limited Resources.” The extended abstracts of USGS authors were peer reviewed and approved for publication by the U.S. Geological Survey. Articles submitted by university researchers and other federal and state agencies did not go through the formal USGS peer review and approval process, and therefore may not adhere to our editorial standards or stratigraphic nomenclature and is not research conducted or data collected by the USGS. However, all articles had at a minimum of two peer reviews, and all articles were edited for consistency of appearance in the published Proceedings. The use of trade, firm or product names in any article is for descriptive purposes only and does not imply endorsement by the U.S. Government. The USGS, Office of Groundwater, provides technical support for the Karst Interest Group website and public availability of the Proceedings from these workshops, and the USGS Groundwater Resources Program funds the publication costs. Finally, the cover illustration is the work of Ann Tihansky, USGS, used since the first KIG workshop in 2000.

Scientific Investigations Report

U.S. Geological Survey Karst Interest Group Proceedings, San Antonio, Texas, May 16–18, 2017

Introduction and Acknowledgments Karst aquifer systems are present throughout parts of the United States and some of its territories, and have developed in carbonate rocks (primarily limestone and dolomite) and evaporites (gypsum, anhydrite, and halite) that span an interval of time encompassing more than 550 million years. The depositional environments, diagenetic processes, post-depositional tectonic events, and geochemical weathering processes that form karst aquifers are varied and complex. These factors involve biological, chemical, and physical changes that when combined with the diverse climatic regimes in which karst development has taken place, result in the unique dual- or triple-porosity nature of karst aquifers. These complex hydrogeologic systems typically represent challenging and unique conditions to scientists attempting to study groundwater flow and contaminant transport in these terrains. The dissolution of carbonate rocks and the subsequent development of distinct and beautiful landscapes, caverns, and springs have resulted in the most exceptional karst areas being designated as national or state parks. Tens of thousands of similar areas in the United States have been developed into commercial caverns and known privately owned caves. Both public and private properties provide access for scientists to study the flow of groundwater in situ . Likewise, the range and complexity of landforms and groundwater flow systems associated with karst terrains are enormous, perhaps more than for any other aquifer type. Karst aquifers and landscapes that form in tropical areas, such as the cockpit karst along the north coast of Puerto Rico, differ greatly from karst landforms in more arid climates, such as the Edwards Plateau in west-central Texas or the Guadalupe Mountains near Carlsbad, New Mexico, where hypogenic processes have played a major role in speleogenesis. Many of these public and private lands also contain unique flora and fauna associated with these karst hydrogeologic systems. As a result, numerous federal, state, and local agencies have a strong interest in the study of karst terrains. Many of the major springs and aquifers in the United States have developed in carbonate rocks, such as the Floridan aquifer system in Florida and parts of Alabama, Georgia, and South Carolina; the Ozark Plateaus aquifer system in parts of Arkansas, Kansas, Missouri, and Oklahoma; and the Edwards-Trinity aquifer system in west-central Texas. These aquifers, and the springs that discharge from them, serve as major water-supply sources and form unique ecological habitats. Competition for the water resources of karst aquifers is common, and urban development and the lack of attenuation of contaminants in karst areas due to dissolution features that form direct pathways into karst aquifers can impact the ecosystem and water quality associated with these aquifers. The concept for developing a platform for interaction among scientists within the U.S. Geological Survey (USGS) working on karst-related studies evolved from the November 1999 National Groundwater Meeting of the USGS. As a result, the Karst Interest Group (KIG) was formed in 2000. The KIG is a loose-knit, grass-roots organization of USGS and non-USGS scientists and researchers devoted to fostering better communication among scientists working on, or interested in, karst science. The primary mission of the KIG is to encourage and support interdisciplinary collaboration and technology transfer among scientists working in karst areas. Additionally, the KIG encourages collaborative studies between the different mission areas of the USGS as well as with other federal and state agencies, and with researchers from academia and institutes. To accomplish its mission, the KIG has organized a series of workshops that have been held near nationally important karst areas. To date (2017) seven KIG workshops, including the workshop documented in this report, have been held. The workshops typically include oral and poster sessions on selected karst-related topics and research, as well as field trips to local karst areas. To increase non-USGS participation an effort was made for the workshops to be held at a university or institute beginning with the fourth workshop. Proceedings of the workshops are published by the USGS and are available online at the USGS publications warehouse https://pubs.er.usgs.gov/ by using the search term “karst interest group.” The first KIG workshop was held in St. Petersburg, Florida, in 2001, in the vicinity of the large springs and other karst features of the Floridan aquifer system. The second KIG workshop was held in 2002, in Shepherdstown, West Virginia, in proximity to the carbonate aquifers of the northern Shenandoah Valley, and highlighted an invited presentation on karst literature by the late Barry F. Beck of P.E. LaMoreaux and Associates. The third KIG workshop was held in 2005, in Rapid City, South Dakota, near evaporite karst features in limestones of the Madison Group in the Black Hills of South Dakota. The Rapid City KIG workshop included field trips to Wind Cave National Park and Jewel Cave National Monument, and featured a presentation by Thomas Casadevall, then USGS Central Region Director, on the status of Earth science at the USGS. The fourth KIG workshop in 2008 was hosted by the Hoffman Environmental Research Institute and Center for Cave and Karst Studies at Western Kentucky University in Bowling Green, Kentucky, near Mammoth Cave National Park and karst features of the Chester Upland and Pennyroyal Plateau. The workshop featured a late-night field trip into Mammoth Cave led by Rickard Toomey and Rick Olsen, National Park Service. The fifth KIG workshop in 2011 was a joint meeting of the USGS KIG and University of Arkansas HydroDays, hosted by the Department of Geosciences at the University of Arkansas in Fayetteville. The workshop featured an outstanding field trip to the unique karst terrain along the Buffalo National River in the southern Ozarks, and a keynote presentation on paleokarst in the United States was delivered by Art and Peggy Palmer. The sixth KIG workshop was hosted by the National Cave and Karst Research Institute (NCKRI) in 2014, in Carlsbad, New Mexico. George Veni, Director of the NCKRI, served as a co-chair of the workshop with Eve Kuniansky of the USGS. The workshop featured speaker Dr. Penelope Boston, Director of Cave and Karst Studies at New Mexico Tech, Socorro, and Academic Director at the NCKRI, who addressed the future of karst research. The field trip on evaporite karst of the lower Pecos Valley was led by Lewis Land (NCKRI karst hydrologist), and the field trip on the geology of Carlsbad Caverns National Park was led by George Veni. This current seventh KIG workshop is being held in San Antonio at the University of Texas at San Antonio (UTSA). This 2017 workshop is being hosted by the Department of Geological Sciences’ Student Geological Society (SGS), and student chapters of the American Association of Petroleum Geologists (AAPG) and Association of Engineering Geologists (AEG), with support by the UTSA Department of Geological Sciences and Center for Water Research. The UTSA student chapter presidents, Jose Silvestre (SGS), John Cooper (AAPG), and Tyler Mead (AEG) serve as co-chairs of the 2017 workshop with Eve Kuniansky of the USGS. The technical session committee is chaired by Eve Kuniansky, USGS, and includes Michael Bradley, Tom Byl, Rebecca Lambert, John Lane, and James Kaufmann, all USGS, and Patrick Tucci, retired USGS. The logistics committee includes Amy Clark, Yongli Gao, and Lance Lambert (Department Chair), UTSA Department of Geological Sciences; and Ryan Banta and Allan Clark, USGS, San Antonio, Texas. The field trip committee is chaired by Allan Clark and includes Amy Clark, Yongli Gao, and Keith Muehlestein, UTSA; Marcus Gary, Edwards Aquifer Authority and University of Texas at Austin; Ron Green, Southwest Research Institute; Geary Schindel, Edwards Aquifer Authority; and George Veni, NCKRI. Additionally, two organizations have assisted the UTSA student chapters in hosting the meeting by donating funds to the chapters: the Edwards Aquifer Authority, San Antonio, Texas, and the Barton Springs Edwards Aquifer Authority, Austin, Texas. Additionally, Yongli Gao, Center for Water Research and Department of Geological Sciences, UTSA, helped develop sessions on cave and karst research in China for this workshop. These proceedings could not have been accomplished without the assistance of Lawrence E. Spangler as co-editor who not only has subject matter expertise, but also serves as an editor with the USGS Science Publishing Network. We sincerely hope that this workshop continues to promote future collaboration among scientists of varied and diverse backgrounds, and improves our understanding of karst aquifer systems in the United States and its territories. The extended abstracts of USGS authors were peer reviewed and approved for publication by the USGS. Articles submitted by university researchers and other federal and state agencies did not go through the formal USGS peer review and approval process, and therefore may not adhere to USGS editorial standards or stratigraphic nomenclature. However, all articles had a minimum of two peer reviews and were edited for consistency of appearance in the proceedings. The use of trade, firm or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. The USGS Water Availability and Use Science Program funded the publication costs of the proceedings.

Scientific Investigations Report

Managing conflicts in the River of Grass

Chances are, you would not pack up and move to a new home without first researching the neighborhood, reviewing your finances, and maybe investigating schools nearby. Similarly, you would not buy the first car you find on a magazine cover without first reviewing the technical specifications, exploring your options, and perhaps taking a test drive. Even when making simple purchases online, you probably shop around, check out customer reviews, and scan seller feedback ratings. Rarely do we act on impulse alone when faced with decisions. Rather, we typically start with a vision of what we want before gathering the necessary information, weighing our options, prioritizing our values, and evaluating possible tradeoffs. In making choices that range from lighthearted to life-changing, we use this commonsense strategy every day of our lives. Environmental decision-making is no different. For decades, natural resource managers faced with complicated problems have made decisions by breaking them into smaller components that are easier to approach. And like our day-to day decisions, the process is ideally guided by a shared vision of the future. However, it can become complicated when multiple, seemingly disparate views on what the future should hold emerge. As Lewis Carroll once wrote, “If you don’t know where you are going, any road will get you there.” Divining a vision of the future for the Florida Everglades and making supporting decisions is a complex undertaking. But unlike Alice thrust through the looking glass, we have tools and techniques to help us make sense of it all. The application of tools to help decision-making can move us closer to the successful restoration of this imperiled landscape.

Florida

Species and population variation to salinity stress in Panicum hemitomon, Spartina patens, and Spartina alterniflora: Morphological and physiological constraints

Panicum hemitomon, Spartina patens, and Spartina alterniflora are wide-spread dominant grasses of fresh, brackish, and salt marsh plant communities, respectively. Our previous research identified significant intraspecific variation in salt tolerance and morphology among populations within each species. In this study our objectives were to determine shorter-term physiological/biochemical responses to salinity stress and identify potential indicators of salt tolerance, with the ultimate goal of discerning similarities and differences in the mechanisms of salinity stress resistance. We subjected a subset of six populations within each species, ranging from high to low salt tolerance, to sublethal salinity levels (4, 20, and 30 ppt, respectively, for species) and monitored physiological and growth responses after 1 week (early harvest) and 5 weeks (late harvest). In all three species sublethal salinity levels generally resulted in significantly reduced net CO2 assimilation, leaf expansion, midday leaf xylem pressure, water use efficiency, and live and total biomass; and significantly increased leaf Na+/K+ ratio, leaf proline, leaf glycine betaine, leaf sucrose, root-to-shoot ratio, and dead:total aboveground biomass ratio. All three species displayed significant population (intraspecific) variation in net CO2 assimilation, leaf expansion, water use efficiency, midday leaf xylem pressure, leaf proline, leaf glycine betaine (except Panicum, where it could not be accurately determined), leaf Na+/K+ ratio, leaf sucrose, total plant biomass, dead:total aboveground biomass ratio, and root-to-shoot ratio. General indicators of salt tolerance (regardless of species) included high net CO2 assimilation rates and water use efficiencies, and low ratios of root-to-shoot and dead:total aboveground biomass. Factor analysis and a-priori linear contrasts revealed some unique differences between species in terms of the relative importance of morphology and physiology in explaining intraspecific variation in salt tolerance. Plant morphology (size attributes) were strongly associated with salt tolerance in P. hemitomon, weakly associated with salt tolerance in S. patens, and not associated with salt tolerance in S. alterniflora. Highly salt-tolerant populations of Spartina alterniflora displayed the greatest ion selectivity (lower leaf Na+/K+ ratios), which was not displayed by the other two species. These results suggest that plant size attributes can be very important in explaining population differences in salt tolerance in glycophytes, but may be independent of salt tolerance in halophytes, which have specialized physiological (and/or anatomical) adaptations that can confer salinity stress resistance through mechanisms such as selective ion exclusion and secretion. ?? 2001 Elsevier Science B.V. All rights reserved.

Conference Paper

Annual grass invasion is transforming the sagebrush biome’s songbird communities

Novel stressors like climate change and biological invasions alter ecological communities, resulting in changes to ecosystem services and biodiversity (that is, ecological transformation). Most ecological transformation research focuses on plants, but animals are likely affected by and plausibly mediate the extent, impact, and pace of transformations. In western North America, where invasive annual grasses are transforming sagebrush-steppe ecosystems, we quantified how transformation in vegetation drives songbird community change. We hypothesized that transformation of vegetation communities via invasion alters wildlife communities by favoring generalists over specialists, albeit with extinction debts that may temporarily obscure transformations’ consequences. Although local-scale songbird diversity increased with grass invasion, we found that this shift was accompanied by ongoing reorganization of songbird communities at larger scales (across the sagebrush biome), driven by heterogeneous impacts of invasion among species and guilds. Through our biome-wide analysis, we were also able to identify high-priority regions for conservation of sensitive songbird species. Our research provides evidence that wildlife communities are transforming alongside vegetation communities and offers insight into the approaches required to quantify nascent community turnover.

western United States

An accurate method for measuring triploidy of larval fish spawns

A standard flow cytometric protocol was developed for estimating triploid induction in batches of larval fish. Polyploid induction treatments are not guaranteed to be 100% efficient, thus the ability to quantify the proportion of triploid larvae generated by a particular treatment helps managers to stock high-percentage spawns and researchers to select treatments for efficient triploid induction. At 3 d posthatch, individual Grass Carp Ctenopharyngodon idella were mechanically dissociated into single-cell suspensions; nuclear DNA was stained with propidium iodide then analyzed by flow cytometry. Following ploidy identification of individuals, aliquots of diploid and triploid cell suspensions were mixed to generate 15 levels (0–100%) of known triploidy ( n = 10). Using either 20 or 50 larvae per level, the observed triploid percentages were lower than the known, actual values. Using nonlinear regression analyses, quadratic equations solved for triploid proportions in mixed samples and corresponding estimation reference plots allowed for predicting triploidy. Thus, an accurate prediction of the proportion of triploids in a spawn can be made by following a standard larval processing and analysis protocol with either 20 or 50 larvae from a single spawn, coupled with applying the quadratic equations or reference plots to observed flow cytometry results. Due to the universality of triploid DNA content being 1.5 times the diploid level and because triploid fish consist of fewer cells than diploids, this method should be applicable to other produced triploid fish species, and it may be adapted for use with bivalves or other species where batch analysis is appropriate.

North American Journal of Aquaculture