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Endophytic bacteria in grass crop growth promotion and biostimulation

Plants naturally carry microbes on seeds and within seeds that may facilitate development and early survival of seedlings. Some crops have lost seed-vectored microbes in the process of domestication or during seed storage and seed treatment. Biostimulant microbes from wild plants were used by pre-modern cultures to re-acquire beneficial seed microbes. Today some companies have developed or are developing the use of microbes obtained from soils or plant sources to stimulate plant development and growth. Many of these biostimulant microbes are endophytic in plants. Biostimulant products also include humic substances, which appear to function as signal molecules in plants, triggering increased internalization of soil microbes into root cells and tissues. In addition, protein coatings on seeds fuel the growth of seed surface-vectored microbes, increasing microbial activity around and within roots. In this article, we provide evidence of the endophytic nature of many biostimulant microbes, and suggest that many of the beneficial effects of microbial biostimulants stem from their action as endophytes or as participants or stimulants of rhizophagy cycle activity.

Grass Research

Is "weediness" and "invasiveness" of weeds a function of the plant microbiome?

Over the past several decades, the extent to which microbes enhance plant development and health has become clearer; however, this has not been explored in terms of the aggressiveness and hardiness of weedy plants. In this review, we explore the hypothesis that many features of weeds and invasive plants are related to the activities of plant microbiomes. Microbes contribute to weed growth, fecundity, and fitness. They also play roles in soils, and in plants as endophytes, where they modulate plant development and protect the host from pathogens, insects, animals, and abiotic stresses. In addition, the adaptability and hardiness of weeds partly stem from the effects of endophytes on plant gene expression and genetic diversity. Weed control often involves multiple applications of herbicides or other treatments that can be costly and destructive. Weed and invasive plant control for agriculture and environment goes beyond monetary costs to negative impacts on people, animals and environment. However, with a more complete knowledge of the roles played by microbes, their symbiotic interactions may be altered to diminish aggressive traits less expensively and with fewer non target effects on environmental, human and animal health.

Grass Research

Evaluation of Ziram as an oral toxic bait chemical for control of grass carp Ctenopharyngodon idella

The grass carp, Ctenopharyngodon idella , is an invasive species in North America that has been recorded in 45 states with breeding populations in several major river basins. Established populations of grass carp have had cascading, negative effects on aquatic ecosystem structure and function. Oral piscicide baits have been examined as a potential method to manage invasive grass carp. Our goal was to examine the oral toxicity of the dimethyl-dithiocarbamate fungicide, Ziram, to grass carp. Three toxicity experiments used different carriers to deliver single Ziram doses ranging from 0.25 to 250 mg/kg by gavage. No acute mortality was observed when grass carp were gavaged with Ziram at the highest concentrations dissolved in ethanol at 40 mg/kg, suspended in dimethyl sulfoxide (DMSO) at 250 mg/kg, or suspended in polyethylene glycol (PEG) at 150 mg/kg. Ziram exposure through intraperitoneal injection resulted in acute mortality at 150 mg/kg potentially due to increased residence time in the peritoneal cavity and thereby greater opportunity for absorption. These results indicate that Ziram is acutely toxic to grass carp, however, additional research is required to formulate a successful novel grass carp toxicant that can be used to target the invasive species while minimizing effects on non-target fish species.

Management of Biological Invasions

Grassland birds wintering at U.S. Navy facilities in southern Texas

Grassland birds have undergone widespread decline throughout North America during the past several decades. Causes of this decline include habitat loss and fragmentation because of conversion of grasslands to cropland, afforestation in the East, brush and shrub invasion in the Southwest and western United States, and planting of exotic grass species to enhance forage production. A large number of exotic plant species, including grasses, have been introduced in North America, but most research on the effects of these invasions on birds has been limited to breeding birds, primarily those in northern latitudes. Research on the effects of exotic grasses on birds in winter has been extremely limited. This is the first study in southern Texas to examine and compare winter bird responses to native and exotic grasslands. This study was conducted during a period of six years (2003–2009) on United States Navy facilities in southern Texas including Naval Air Station–Corpus Christi, Naval Air Station–Kingsville, Naval Auxiliary Landing Field Waldron, Naval Auxiliary Landing Field Orange Grove, and Escondido Ranch, all of which contained examples of native grasslands, exotic grasslands, or both. Data from native and exotic grasslands were collected and compared for bird abundance and diversity; ground cover, vegetation density, and floristic diversity; bird and vegetation relationships; diversity of insects and arachnids; and seed abundance and diversity. Effects of management treatments in exotic grasslands were evaluated by comparing numbers and diversity of birds and small mammals in mowed, burned, and control areas. To determine bird abundance and bird species richness, birds were surveyed monthly (December–February) during the winters of 2003–2008 in transects (100 meter × 20 meter) located in native and exotic grasslands distributed at all five U.S. Navy facilities. To compare vegetation in native and exotic grasslands, vegetation characteristics were measured during 2003–2008 in the same transects used for bird surveys and included five measures of ground cover, plus estimates of plant species richness, vegetation density (visual obstruction) at two different heights, and shrub numbers. These data, plus seasonal rainfall, were then used to evaluate components of variation in native and exotic grasslands. Relations between total bird numbers and bird species richness with environmental variation in native and exotic grasslands were compared. To compare diversity of arthropods in native and exotic grasslands, insects and arachnids were collected using three different methodologies (standardized sweep-net, random sweep-net, and pitfall traps) during four seasons, (2005–2006), at Naval Air Station–Corpus Christi, Naval Auxiliary Landing Field Waldron, and Naval Air Station–Kingsville. To compare seed abundance and diversity between native and exotic grasslands, seeds were collected for two winters (2004–2006) at Naval Air Station–Corpus Christi and Naval Air Station–Kingsville. To evaluate effects of management on grassland vertebrates, abundance and diversity of birds and small mammals were estimated and compared in exotic grasses subjected to mowing, burning, or no active management (control) for one full year (2008–2009). Observations were made of 1,044 birds of 30 species in grassland transects during five winters. The Savannah Sparrow ( Passerculus sandwichensis ) was the most common bird, which, with 644 detections, accounted for 63 percent of all individuals identified to species. Meadowlarks ( Sturnella spp. ) and Le Conte’s Sparrows ( Ammodramus leconteii ) were the second (10 percent) and third (7 percent) most abundant bird species, respectively. Six of the seven most abundant species detected in grasslands were grassland species, and their numbers accounted for 87 percent of all birds, but 20 of the 30 species (67 percent) that used grasslands were not grassland species. Seven species observed in grassland transects during the study were Species of Conservation Concern: Le Conte’s Sparrow, Sedge Wren ( Cistothorus platensis ), Grasshopper Sparrow ( Ammodramus savannarum ), Long-billed Curlew ( Numenius americanus ), Sprague’s Pipit ( Anthus spragueii ), Cassin’s Sparrow ( Aimophila cassinii ), and Loggerhead Shrike ( Lanius ludovicianus ). Native grasslands consistently supported greater bird species richness than exotic grasslands. In one winter, exotic grasslands supported more birds than native grasslands. Native grasslands were determined to have more forb cover, more bare ground, and greater plant species richness than exotic grasslands, whereas exotic grasslands were characterized by more grass cover and relatively greater vegetation density during dry years. Not only did these individual measures differ between native and exotic grasslands, but components of variation also differed. In native grasslands, grass density and cover contributed more to variation, whereas in exotic grasslands, non-grass vegetation was a greater component of variation. Total bird numbers and bird species richness in native grasslands were related to the principal component that contained a measure of litter cover. Total bird numbers and bird species richness in exotic grasslands indicated no significant relationships with any of the principal components of variation. The two most common insect orders in native grasslands were Hymenoptera and Coleoptera, which accounted for 42 percent of all insects. The two most common insect orders in exotic grasslands were Hemiptera and Homoptera, which accounted for about 80 percent of all insects. Insect family richness was greater in exotic grasslands than in native grasslands in two of four seasons. Proportions of arachnid families were similar in native and exotic grasslands, but arachnid family richness was greater in exotic grasslands than in native grasslands. Abundance of seeds was greater in exotic than in native grasslands. However, seed diversity was greater in native grasslands than in exotic grasslands. Among the three types of management (mowed, burned, and control) applied to exotic grasses, birds were most abundant in the mowed area. Sedge Wrens, however, were never encountered in mowed sites. Meadowlarks were similarly abundant in all treatments, but Le Conte’s Sparrows were detected only in the control (unmanaged) area. Hispid cotton rats ( Sigmodon hispidus ) accounted for 93 percent of all rodent captures, with the number of captures peaking December through February. Hispid cotton rat numbers and total rodent numbers were greatest in control and pre-burn areas, and lowest in the mowed area. Mammal diversity, however, was greatest in the mowed habitat. Native and exotic grasslands differed essentially in all categories (bird numbers and diversity, vegetation characteristics, components of variation, diversity of insects and arachnids, and seed abundance and diversity) used to measure and compare them. This indicates that fundamental ecosystem processes have been altered after native grasslands have undergone invasion and ultimate domination by exotic grass species. Future research in Texas grassland ecosystems is essential because: 1) Texas sustains more area in grasslands than any other state or province in the Central Flyway; 2) Texas serves as the winter destination or migration pathway for hundreds of species of birds, including winter residents and Neotropical migrants; 3) ecology, distribution, and numbers of grassland birds wintering in southern latitudes of the United States remains poorly understood; and 4) climate change threatens to further accelerate advances of invading grass species.

Open-File Report

A review of grass carp and related species literature on diet, behavior, toxicology, and physiology focused on informing development of controls for invasive grass carp populations in North America

Grass carp ( Ctenopharyngodon idella ) are globally important in aquaculture and aquatic vegetation control. However, escaped grass carp have established invasive populations. A targeted keyword search was performed on a carp (order: Cypriniformes) literature database maintained by the U.S. Geological Survey to identify literature relevant to grass carp. Additional sources cited in reviewed documents and provided by numerous reviewers were also included. There were three focus areas designed to provide support for invasive grass carp management: (1) diet and behavior; (2) physiological constraints, toxicity, and biology; and (3) gut physiology. Each focus area provides information to guide development of potential pathways for invasive grass carp control. Information from other carp species was used to fill in gaps where grass carp information was lacking and provide additional, potential research directions. Diet-related information included food selection and aquacultural diet formulations. Behavioral information included stimuli and non-physical barriers to attract, repel, or stop movement. Physiological constraints, toxicology, reproductive control, and biological control provide a research review for control options. Gut physiology and related control pathways provide knowledge to improve toxin or pathogen delivery. This review provides a basis for developing approaches and research for controlling invasive grass carp populations, aquaculture, and native population management.

Fishes

Comparative olfactory and behavioral response of grass carp ( Ctenopharyngodon idella ), bighead carp ( Hypophthalmichthys nobilis ), silver carp ( H. molitrix ) and black carp ( Mylopharyngodon piceus ) to chemical stimuli

Grass carp ( Ctenopharyngodon idella ), bighead carp ( Hypophthalmichthys nobilis ), silver carp (H. molitrix), and black carp ( Mylopharyngodon piceus ), collectively “invasive carp,” represent an ongoing management problem in the Mississippi and Missouri River basins of North America. Development of strategies to control their populations are also ongoing. As a follow-up to previous research, we tested two amino acids and an amino acid-based commercial feeding stimulant as olfactory stimulants that may attract or repel these species. We employed a two-pronged approach to test how invasive carp respond to the olfactory stimulants, using an electro-olfactogram (EOG) to measure olfactory response and a tank with a stimulant in one half to test behavioral response. Consistent with previous research, we found grass carp had a higher magnitude of EOG response to stimulants compared to bighead and, marginally, silver carp. Bighead, silver, and black carp did not differ from each other in EOG response. Grass carp produced significantly greater lysine and a marginally greater alanine EOG response than bighead and silver carp and significantly greater commercial feeding stimulant response than bighead carp. Black carp had marginally greater lysine response than bighead carp. In the behavioral tests, grass carp generally avoided the stimuli tested, while bighead carp were generally attracted to stimuli. Neither black nor silver carp showed a clear attraction or avoidance toward the stimuli tested. To further evaluate these solutions for management applications additional testing in ponds or open water may be necessary.

Management of Biological Invasions

The influence of precipitation, vegetation and soil properties on the ecohydrology of sagebrush steppe rangelands on the INL site

The INL Site and other landscapes having sagebrush steppe vegetation are experiencing a simultaneous change in climate and floristics that result from increases in exotic species. Determining the separate and combined/interactive effects of climate and vegetation change is important for assessing future changes on the landscape and for hydrologic processes. This research uses the 72 experimental plots established and initially maintained for many years as the “Protective Cap Biobarrier Experiment” by Dr. Jay Anderson and the Stoller ESER program, and the experiment is also now referred to as the “INL Site Ecohydrology Study.” We are evaluating long-term impacts of different plant communities commonly found throughout Idaho subject to different precipitation regimes and to different soil depths. Treatments of amount and timing of precipitation (irrigation), soil depth, and either native/perennial or exotic grass vegetation allow researchers to investigate how vegetation, precipitation and soil interact to influence soil hydrology and ecosystem biogeochemistry. This information will be used to improve a variety of models, as well as provide data for these models.

Report

Estimating the extent of impervious surfaces and turf grass across large regions

The ability of researchers to accurately assess the extent of impervious and pervious developed surfaces, e.g., turf grass, using land-cover data derived from Landsat satellite imagery in the Chesapeake Bay watershed is limited due to the resolution of the data and systematic discrepancies between developed land-cover classes, surface mines, forests, and farmlands. Estimates of impervious surface and turf grass area in the Mid-Atlantic, United States that were based on 2006 Landsat-derived land-cover data were substantially lower than estimates based on more authoritative and independent sources. New estimates of impervious surfaces and turf grass area derived using land-cover data combined with ancillary information on roads, housing units, surface mines, and sampled estimates of road width and residential impervious area were up to 57 and 45% higher than estimates based strictly on land-cover data. These new estimates closely approximate estimates derived from authoritative and independent sources in developed counties.

Delaware;Maryl;New York;Pennsylvania

A food web modeling assessment of Asian Carp impacts in the Middle and Upper Mississippi River, USA

The invasion of non-native fishes has caused a great detriment to many of our native fishes. Since the introduction of invasive carps, such as Silver, Bighead, Common and Grass Carp , managers and researcher have been struggling to remove these species while also hypothesizing the detriment of further invasion. This study developed a food web model of four locations on the Mississippi River and used those models to assess the impacts of two scenarios: carp removal and carp invasion. In the Middle Mississippi River where these invasive carps are already present, the models found that it would take a sustained exploitation of up to 30% of initial biomass over an extended period to remove Grass Carp and up to 90% removal of initial biomass to remove Silver and Bighead Carp. In the locations where Silver, Bighead, and Grass Carp are not yet established (i.e., Pools 4,8, and 13) the invasion of these species could cause declines from 10 to 30% in initial biomass of native fishes as well as already established nonnative invasive species.

Illinois, Iowa, Minnesota, Missouri, Wisconsin

Dead giveaway: Rising mortality rates suggest effectiveness of Lake Erie grass carp (Ctenopharyngodon idella) response

Grass carp ( Ctenopharyngodon idella ) are large, invasive fish that threaten Lake Erie’s economy and ecosystem. Incidental catches of grass carp have occurred since the 1980s in Lake Erie, while multi-day removal events were carried out in 2014 and 2017. To mitigate ecosystem impacts, a large-scale, multi-agency response to remove as many grass carp as possible from the Lake Erie basin (“strike teams”) began in 2018 and has increased every year. To date, total annual removals of fish has been the primary measure of progress; however, total annual removals do not indicate how efforts are affecting the grass carp population. Population vital rates, such as mortality rate, can indicate population demographic changes and may provide an alternative approach to measure how removals have impacted the grass carp population. We estimated annual mortality rates using 553 grass carp, representing 82.9 % of all grass carp removed in the Lake Erie basin, using a hierarchical catch-curve model and catch-at-age data from 2014 to 2022. Annual average mortality rates were initially low (4.3 %) and increased between 2017 and 2022 with the highest mortality (13.6 %) observed in 2021. Positive correlations between mortality and the number of fish harvested per year suggest that removals may be driving increases in the grass carp mortality rate. This increase in mortality rate shows promise for controlling the spread of grass carp within the Lake Erie ecosystem. This research supports the needs of fishery managers to better understand grass carp population dynamics and the adaptive management framework identified in the Lake Erie Grass Carp Adaptive Response Strategy.

Lake Erie

Extracting exotic annual grass phenology and climate relations in western U.S. rangeland ecoregions

This research builds upon the extensive body of work to model exotic annual grass (EAG) characteristics and invasion. EAGs increase wildland fire risk and intensifies wildland fire behavior in western U.S. rangelands. Therefore, understanding characteristics of EAG growth increases understanding of its dynamics and can inform rangeland management decisions. To better understand EAG phenology and spatial distribution, monthly weather (precipitation, minimum and maximum temperature) variables were analyzed for 24 level III ecoregions. This research characterizes EAGs’ phenology identified by a normalized difference vegetation index (NDVI) threshold-based interpolation technique. An EAG phenology metric model was used to estimate a growing season dynamic for the years 2017–2021 for shrub and herbaceous land cover types in the western conterminous United States (66% of the area). The EAG phenology metrics include six growing season metrics such as start of season time, end of season time, and time of maximum NDVI during the growing season. The models’ cross validation results for Pearson’s r ranged from 0.88 to 0.95. Increased understanding of the effects that weather conditions have on EAG growth and spatial distribution can help land managers develop time-sensitive plans to protect entities deemed valuable to society like native habitat, wildlife, recreational areas, and air quality.

western rangeland ecoregions

Determinants of mallard and gadwall nesting on constructed islands in North Dakota

Constructed islands with adequate nesting cover provide secure nesting sites for ducks because islands restrict access by mammalian predators. These islands are costly to construct and should be placed in areas that ensure the greatest use by nesting ducks. We studied mallard (Anas platyrhynchos) and gadwall (A. strepera) nesting on constructed islands in North Dakota in 1996 (n = 20) and 1997 (n = 22) to evaluate factors—particularly amount of perennial grass cover in the surrounding landscape and density of breeding pairs—that possibly influence numbers of initiated nests. We also examined effects of island characteristics, such as island vegetation, on numbers of nests. Numbers of mallard and gadwall nests on islands were negatively related to amounts of perennial grass cover in the surrounding uplands. Numbers of mallard nests were positively related to percentages of tall dense cover on islands. We found no effects of breeding-pair density on numbers of nests initiated by either species, possibly because breeding pairs were abundant on all study sites. Percent shrub cover on islands was a better predictor of island use than was percent tall dense cover. Island use by these species increased with island age and distance from mainland shore. Amounts of perennial cover in landscapes should be primary considerations in determining where to build islands. Our data suggest that use of islands by nesting mallards and gadwalls is greatest in landscapes with little perennial grass cover (i.e., high amounts of cropland). Other researchers documented a positive relation between nest success in upland covers and amount of perennial grass cover in the landscape. Therefore, islands constructed in landscapes with little perennial cover should provide greater gains in duck recruitment rates than islands constructed in landscapes with greater amounts of perennial grass cover.

Journal of Wildlife Management

On the importance of ichthyoplankton monitoring for invasive grass carp control in the Laurentian Great Lakes

Grass carp ( Ctenopharyngodon idella ) is an invasive herbivore observed in small numbers in the Laurentian Great Lakes since the 1980 s with records from all lakes except Lake Superior. Identification of diploid, age-1 + grass carp from the Sandusky River, a Lake Erie tributary, in 2012, prompted targeted efforts to evaluate the status of grass carp reproduction in the Lake Erie Basin. In 2014, researchers from the U.S. Geological Survey and University of Toledo began surveys to assess grass carp spawning in the Great Lakes, with the first fertilized eggs discovered in 2015 in the Sandusky River. Eight Great Lakes tributaries in the United States have been surveyed for evidence of grass carp spawning, identifying three confirmed spawning tributaries (Sandusky, Maumee, and Huron Rivers in Ohio). Initial work identified specific flow and temperature conditions that likely stimulate spawning. Egg and larval drift modeling estimated spawning locations based on egg development and flow characteristics, which helped removal crews locate and target spawning fish in the Sandusky River and later, the Maumee River. The research synthesized herein demonstrates the value of ichthyoplankton research to inform control of emerging invasive species and could inform management of other invasive carps with similar reproductive life histories, should they enter the Great Lakes.

Michigan, Ohio

Strategic plan for the Coordinated Intermountain Restoration Project

In 1982, the Bureau of Land Management’s (BLM) Idaho State Office began the Intermountain Greenstripping and Rehabilitation Research Project (IGRRP), or the “Greenstripping Program,” to investigate plant materials and technologies that can reduce wildfire incidence and improve rehabilitation practices. Rehabilitation is normally applied as a reactive process to wildfires, yet land managers in the Great Basin wish to become proactive by replacing fire-prone invasive annual grasses with native plants. The Coordinated Intermountain Restoration Project (CIRP) evolved from the Greenstripping Program to conduct research studies and provide technical assistance on restoration of native ecosystems on rangelands that are infested with invasive annual grasses or other invasive or noxious weeds. To accomplish this objective, the CIRP will promote the understanding of ecosystem disturbance dynamics as well as evaluate plant materials, site preparation techniques, weed control methods, seeding equipment, management methods, and monitoring techniques for restoration projects. The CIRP will not address the restoration of forested or woodland (juniper [Juniperus]) ecosystems. It will include a component on fuel management to reduce the impacts of wildfires on semiarid rangeland ecosystems where exotic annual grasses provide the fuel. The people who will benefit directly from this research include land managers and users of public and private lands in the northern Great Basin, the Columbia Plateau, and the Snake River Plain. The CIRP will provide an integration framework for a multidisciplinary approach to research with numerous opportunities for input and collaboration. The U.S. Geological Survey will initially dedicate approximately \$1 million over 5 years (about \$200,000 per year) to jump-start this effort. U.S. Geological Survey funds will establish a science advisory board to oversee the project. This board will contain members of Federal research and management agencies within the region. U.S. Geological Survey funds will support (1) continued development of VegSpec, a computer program that is a restoration expert system, (2) research to examine changes in ecosystem processes when native plant-dominated communities shift to communities dominated by exotic annual grasses, and (3) research to address mechanisms for establishing native plants in locations dominated by exotic annual grasses. Through these initial funds, USGS hopes to leverage additional research with other agencies (e.g., BLM’s Great Basin Restoration Initiative or the Native Plant Materials Development Project, which is an interagency program to supply and manage native plant materials for restoration and rehabilitation on Federal lands) or funding organizations (e.g., the U.S. Department of Agriculture’s [USDA] National Research Initiative Competitive Grants Program, or the USDA’s and U.S. Department of the Interior’s [USDOI] Joint Fire Science Program), and to obtain additional research partners (e.g., university or Federal scientists) willing to expand this effort to address all aspects of this strategic plan.

Information and Technology Report

C 3 and C 4 plant responses to increased temperatures and altered monsoonal precipitation in a cool desert on the Colorado Plateau, USA

Dryland ecosystems represent >40 % of the terrestrial landscape and support over two billion people; consequently, it is vital to understand how drylands will respond to climatic change. However, while arid and semiarid ecosystems commonly experience extremely hot and dry conditions, our understanding of how further temperature increases or altered precipitation will affect dryland plant communities remains poor. To address this question, we assessed plant physiology and growth at a long-term (7-year) climate experiment on the Colorado Plateau , USA, where the community is a mix of shallow-rooted C 3 and C 4 grasses and deep-rooted C 4 shrubs. The experiment maintained elevated-temperature treatments (+2 or +4 °C) in combination with altered summer monsoonal precipitation (+small frequent precipitation events or +large infrequent events). Increased temperature negatively affected photosynthesis and growth of the C 3 and C 4 grasses, but effects varied in their timing: +4 °C treatments negatively affected the C 3 grass early in the growing season of both years, while the negative effects of temperature on the C 4 grass were seen in the +2 and +4 °C treatments, but only during the late growing season of the drier year. Increased summer precipitation did not affect photosynthesis or biomass for any species, either in the year the precipitation was applied or the following year. Although previous research suggests dryland plants, and C 4 grasses in particular, may respond positively to elevated temperature, our findings from a cool desert show marked declines in C 3 and C 4 photosynthesis and growth, with temperature effects dependent on the degree of warming and growing-season precipitation.

Utah

Science and innovation for battling invasive carp

The U.S. Geological Survey (USGS) is the Federal Government’s independent research agency that conducts research to inform management of invasive species. Bighead, black, grass, and silver carp, which are native to China, were originally stocked in aquaculture facilities to control algae, snails, and vegetation. These species have invaded the Mississippi River and are now established throughout the lower and middle Mississippi River Basins and some of its large tributaries where they damage ecosystems and harm economies. A reproducing population of grass carp in Lake Erie is a risk to the $7 billion Great Lakes fishery. The USGS is leading research efforts on risk assessment and on early detection of new invasions. The USGS is also developing and evaluating harvest methods, barriers and deterrents, and baits/attractants to provide Federal and State management agencies the tools needed to manage and reduce populations of these species.

Alabama, Arkansas, Illinois, Indiana, Iowa, Kansas

The Origins of C4 Grasslands: Integrating Evolutionary and Ecosystem Science

The evolution of the C 4 photosynthetic pathway from the ancestral C 3 pathway in grasses led to the establishment of grasslands in warm climates during the Late Miocene (8 to 3 million years ago). This was a major event in plant evolutionary history, and their high rates of foliage production sustained high levels of herbivore consumption. The past decade has seen significant advances in understanding C 4 grassland ecosystem ecology, and now a wealth of data on the geological history of these ecosystems has accumulated and the phylogeny of grasses is much better known. Edwards et al. (p. 587) review this multidisciplinary research area and attempt to synthesize emerging knowledge about the evolution of grass species within the context of plant and ecosystem ecology.

Science

Assessing water quality from highway runoff at selected sites in North Carolina with the Stochastic Empirical Loading and Dilution Model (SELDM)

In 2015, the U.S. Geological Survey (USGS) entered into a cooperative agreement with the North Carolina Department of Transportation (NCDOT) to develop a North Carolina-enhanced variation of the national Stochastic Empirical Loading and Dilution Model (SELDM) with available North Carolina-specific streamflow and water-quality data and to demonstrate use of the model by documenting selected simulation scenarios. The USGS developed the national SELDM in cooperation with the Federal Highway Administration (FHWA) to provide the tools and techniques necessary for performing stormwater-quality simulations. SELDM uses a stochastic mass-balance approach to estimate combinations of flows, concentrations, and loads of stormwater constituents from the site of interest (often a highway catchment; nonhighway areas, such as a large impervious area at a shopping center complex, also can be used) and the basin upstream from the stormwater outfall to assess the risk for adverse effects of runoff. SELDM also can be used to simulate the effectiveness of volume reduction, hydrograph extension, and water-quality concentration reductions by stormwater best management practices (BMPs), which are designed to help mitigate the effects of runoff on receiving water bodies. Some of the statistical inputs needed for the North Carolina-enhanced SELDM were either calculated or augmented using local or regional data from North Carolina. Streamflow statistics used by SELDM were determined for 266 streamgages across North Carolina on the basis of data available through the 2015 water year. Recession ratio statistics used for triangular hydrographs were also developed for 30 streamgages across the State. The NCDOT identified previous research reports on highway-runoff and BMP studies in North Carolina for review of potential data addition to the national FHWA Highway-Runoff Database (HRDB). Following USGS review of these data, a total of 25,087 event mean concentration values and 1,140 storm events for 39 highway-runoff sites and 195 analytes were uploaded to the national HRDB from six North Carolina highway-runoff research reports and a recent USGS bridge deck runoff study. Using data for 27 streamgages in North Carolina, a total of 57 water-quality transport curves were developed for seven constituents for use in simulating water-quality conditions in the upstream basin. Performance data for three BMPs (bioretention, grass strip or swale, and wetland channel) from NCDOT research data were incorporated into the North Carolina-enhanced SELDM for volume-reduction statistics, including the effectiveness of treating four water-quality constituents (total suspended solids, total nitrogen, total phosphorus, nitrate plus nitrite) and turbidity. Simulations using the North Carolina-enhanced SELDM are presented for two hypothetical upstream basins in the Piedmont ecoregion and one hypothetical highway site to demonstrate how simulations can be used to provide risk-based information about potential effects of stormwater runoff on downstream water quality and the potential for mitigating those risks by using BMPs. The first group of simulations explores the stochastic variability in dilution factors (the ratio of the highway runoff to the total downstream stormflow) for a hypothetical Piedmont rural creek having drainage areas ranging from 1 to 100 square miles. The second group of simulations examines dilution factors based on variations in precipitation, streamflow, and recession ratios for two hypothetical Piedmont upstream basins (rural and urban) where the drainage area was held constant at 25 square miles. These simulations indicate the sensitivity of results to variations in each of the three variables. The third group of simulations examines the effects of varied concentrations in the upstream basin on water-quality conditions downstream from the highway crossing. Variations in upstream water-quality conditions for three constituents (suspended sediment concentration, total nitrogen, and total phosphorus) are based on water-quality transport curves selected from among the 57 curves developed as part of this study to represent low-, medium-, and high-concentration statistics. Simulations completed for this third group also examine the potential effects of grass swale and bioretention BMP treatment on total nitrogen and total phosphorus concentrations in highway runoff. The BMP performance data from the NCDOT research reports were applied in this group of simulations. The stochastic mass-balance approach used in SELDM analyses and simulations provides a strong tool for engineers and water-resource managers to use in exploring a wide range of possible hydrologic and water-quality inputs and their effects on downstream water quality. The results of this study can not only aid engineers and managers in planning for potential adverse effects of runoff at site-specific locations, they can also help the USGS and other Federal and State agencies with oversight responsibilities in stormwater-quality issues to continue gathering data on potential water-quality effects in receiving streams.

North Carolina