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At least 235 records · Page 13Linked to original sources

Reach-scale isotope tracer experiment to quantify denitrification and related processes in a nitrate-rich stream, midcontinent United States

We conducted an in-stream tracer experiment with Br and 15 N-enriched NO 3 - to determine the rates of denitrification and related processes in a gaining NO 3 - -rich stream in an agricultural watershed in the upper Mississippi basin in September 2001. We determined reach-averaged rates of N fluxes and reactions from isotopic analyses of NO 3 - , NO 2 - , N 2 , and suspended particulate N in conjunction with other data in a 1.2-km reach by using a forward time-stepping numerical simulation that included groundwater discharge, denitrification, nitrification, assimilation, and air-water gas exchange with changing temperature. Denitrification was indicated by a systematic downstream increase in the d15N values of dissolved N 2 . The reach-averaged rate of denitrification of surface-water NO 3 - indicated by the isotope tracer was approximately 120 ± 20 µmol m -2 h -1 (corresponding to zero- and first-order rate constants of 0.63 µmol L -1 h -1 and 0.009 h -1 , respectively). The overall rate of NO 3 - loss by processes other than denitrification (between 0 and about 200 µmol m -2 h -1 ) probably was less than the denitrification rate but had a large relative uncertainty because the NO 3 - load was large and was increasing through the reach. The rates of denitrification and other losses would have been sufficient to reduce the stream NO 3 - load substantially in the absence of NO 3 - sources, but the losses were more than offset by nitrification and groundwater NO 3 - inputs at a combined rate of about 500-700 µmol m -2 h -1 . Despite the importance of denitrification, the overall mass fluxes of N 2 were dominated by discharge of denitrified groundwater and air-water gas exchange in response to changing temperature, whereas the flux of N 2 attributed to denitrification was relatively small. The in-stream isotope tracer experiment provided a sensitive direct reach-scale measurement of denitrification and related processes in a NO 3 - -rich stream where other mass-balance methods were not suitable because of insufficient sensitivity or offsetting sources and sinks. Despite the increasing NO 3 - load in the experimental reach, the isotope tracer data indicate that denitrification was a substantial permanent sink for N leaving this agricultural watershed during low-flow conditions.

Limnology and Oceanography↗

Denitrification and hydrologic transient storage in a glacial meltwater stream, McMurdo Dry Valleys, Antarctica

In extreme environments, retention of nutrients within stream ecosystems contributes to the persistence of aquatic biota and continuity of ecosystem function. In the McMurdo Dry Valleys, Antarctica, many glacial meltwater streams flow for only 5–12 weeks a year and yet support extensive benthic microbial communities. We investigated NO 3 − uptake and denitrification in Green Creek by analyzing small‐scale microbial mat dynamics in mesocosms and reach‐scale nutrient cycling in two whole‐stream NO 3 − enrichment experiments. Nitrate uptake results indicated that microbial mats were nitrogen (N)‐limited, with NO 3 − uptake rates as high as 16 nmol N cm −2 h −1 . Denitrification potentials associated with microbial mats were also as high as 16 nmol N cm −2 h −1 . During two whole‐stream NO 3 − −enrichment experiments, a simultaneous pulse of NO 2 − was observed in the stream water. The one‐dimensional solute transport model with inflow and storage was modified to simulate two storage zones: one to account for short time scale hydrologic exchange of stream water into and out of the benthic microbial mat, the other to account for longer time scale hydrologic exchange with the hyporheic zone. Simulations indicate that injected NO 3 − was removed both in the microbial mat and in the hyporheic zone and that as much as 20% of the NO 3 − that entered the microbial mat and hyporheic zone was transformed to NO 2 − by dissimilatory reduction. Because of the rapid hydrologic exchange in microbial mats, it is likely that denitrification is limited either by biotic assimilation, reductase limitation, or transport limitation (reduced NO 2 − is transported away from reducing microbes).

Limnology and Oceanography↗

Radium isotopes in Cayuga Lake, New York: Indicators of inflow and mixing processes

Naturally occurring radium isotopes ( 223 Ra, 224 Ra, 226 Ra, and 228 Ra) were measured in lake and tributary water of Cayuga Lake, New York, during the course of a vernal inflow event in the spring of 2001. A large influx of groundwater, probably from a carbonate aquifer, entered the lake at its extreme southern end early in the vernal inflow event and spread northward, covering an extensive part of the southern end of the lake. The low 228 Ra/ 226 Ra activity ratio of this water mass, compared with bulk lake water, allowed its identification through time. Estimates of mixing with bulk lake water were calculated from changes in the 226Ra content. Groundwater inflow to the lake around the delta of a major tributary was detected on the basis of 223 Ra and 224 Ra activity of lake and tributary water. Inflow of a water mass to the surface of the lake was also detected using 223 Ra and 224 Ra activity. The integrity of this water mass was monitored using short‐lived radium isotopes. Suspended sediment in the lake water is a source of the short‐lived radium isotopes 223 Ra (~2 x 10 −4 dpm L −1 ) and 224 Ra (~3 x x 10 −3 dpm L −1 ), but bottom sediments are a more significant source of 228 Ra. Radium isotopes can be valuable new tools in limnological investigations, allowing detection and monitoring of events and processes such as water inflow and mixing, determining sources of inflowing water, and monitoring introduced water masses as they move within the lake.

New York↗

Toxic Alexandrium blooms in the western Gulf of Maine: The plume advection hypothesis revisited

The plume advection hypothesis links blooms of the toxic dinoflagellate Alexandrium fundyense in the western Gulf of Maine (GOM) to a buoyant plume derived from river outflows. This hypothesis was examined with cruise and moored-instrument observations in 1993 when levels of paralytic shellfish poisoning (PSP) toxins were high, and in 1994 when toxicity was low. A coupled physical-biological model simulated hydrography and A. fundyense distributions. Initial A. fundyense populations were restricted to low-salinity nearshore waters near Casco Bay, but also occurred in higher salinity waters along the plume boundary. This suggests two sources of cells - those from shallow-water cyst populations and those transported to shore from offshore blooms in the eastern segment of the Maine coastal current (EMCC). Observations confirm the role of the plume in A. fundyense transport and growth. Downwelling-favorable winds in 1993 transported the plume and its cells rapidly alongshore, enhancing toxicity and propagating PSP to the south. In 1994, sustained upwelling moved the plume offshore, resulting in low toxicity in intertidal shellfish. A. fundyense blooms were likely nutrient limited, leading to low growth rates and moderate cell abundances. These observations and mechanisms were reproduced by coupled physical-biological model simulations. The plume advection hypothesis provides a viable explanation for outbreaks of PSP in the western GOM, but should be refined to include two sources for cells that populate the plume and two major pathways for transport: one within the low-salinity plume and another where A. fundyense cells originating in the EMCC are transported along the outer boundary of the plume front with the western segment of the Maine coastal current.

Casco Bay, Gulf of Maine↗

Eutrophication of freshwater and marine ecosystems

Initial understanding of the links between nutrients and aquatic productivity originated in Europe in the early 1900s, and our knowledge base has expanded greatly during the past 40 yr. This explosion of eutrophication-related research has made it unequivocally clear that a comprehensive strategy to prevent excessive amounts of nitrogen and phosphorus from entering our waterways is needed to protect our lakes, rivers, and coasts from water quality deterioration. However, despite these very significant advances, cultural eutrophication remains one of the foremost problems for protecting our valuable surface water resources. The papers in this special issue provide a valuable cross section and synthesis of our current understanding of both freshwater and marine eutrophication science. They also serve to identify gaps in our knowledge and will help to guide future research.

Limnology and Oceanography↗

Nitrogen sources and cycling in the San Francisco Bay estuary: A nitrate dual isotopic composition approach

We used the dual isotopic composition of nitrate (δ 15 N and δ 18 O) within the estuarine system of San Francisco (SF) Bay, California, to explore the utility of this approach for tracing sources and cycling of nitrate (NO 2 − ). Surface water samples from 49 sites within the estuary were sampled during July–August 2004. Spatial variability in the isotopic composition suggests that there are multiple sources of nitrate to the bay ecosystem including seawater, several rivers and creeks, and sewage effluent. The spatial distribution of nitrate from these sources is heavily modulated by the hydrodynamics of the estuary. Mixing along the estuarine salinity gradient is the main control on the spatial variations in isotopic composition of nitrate within the northern arm of SF Bay. However, the nitrate isotopic composition in the southern arm of SF Bay exhibited a combination of source mixing and phytoplankton drawdown due mostly to the long residence time during the summer study period. Very low δ 18 O NO3 values (as low as −5.0%) at the Sacramento–San Joaquin River delta region give rise to a wide range of δ 18 ONO3 values in the SF Bay system. The range in δ 18 O NO3 values is more than twice that of δ 15 N NO3 , suggesting that δ 18 O NO3 is an even more sensitive tool for tracing nitrate sources and cycling than δ 15 N NO3 .

Limnology and Oceanography↗

North to Alaska: Evidence for conveyor belt transport of Dungeness crab larvae along the west coast of the United States and Canada

We propose and evaluate the hypothesis that Dungeness crab ( Cancer magister ) larvae from the northwestern coast of the United States and Canada can be transported northward to southeastern Alaska. Larvae collected in southeastern Alaska during May and June 1997–2004 had abundances and stages that varied seasonally, interannually, and spatially. An unexpected presence of late-stage larvae in spring raises a question regarding their origin, and the most plausible explanation is that they hatched off the northern Washington and British Columbia coasts and were transported to southeastern Alaska. Buoy drift tracks support the hypothesis that larvae released off the northern Washington and British Columbia coasts during the peak hatching season can be physically transported to southeastern Alaska, arriving as late-stage larvae in May and June, when local larvae are only beginning to hatch. A northward spring progression of monthly mean 7°C SST isotherms and phytoplankton blooms provide further evidence that environmental conditions are conducive for larval growth and metabolism during the transport period. The proposed larval transport suggests possible unidirectional gene flow between southern and northern populations of Dungeness crabs in southeastern Alaska.

Limnology and Oceanography↗

Characterization of suspended particles in Everglades wetlands

We report the concentration, phosphorus (P) and nitrogen (N) content, and size and chemical fractionation of fine suspended particles (0.2‐100 µm) and colloids (3 kilodalton [kDa]‐0.1 µm) in the surface water of Everglades wetlands along regional and P‐enrichment gradients. Total suspended sediment concentrations ranged from 0.7 to 2.7 mg L −1 . Total particulate P concentrations increased from 0.05 µmol L21 to 0.31 µmol L −1 along the Penrichment gradient. Particles contained from 20% to 43% of total P but <12% of total N in surface water. Dissolved (<0.2 µm) organic N contained about 90% of total N, with the 3‐100‐kDa colloidal size class containing the most N of any size class. The 0.45‐2.7‐µm size fraction held the most particulate P at all sites, whereas particulate N was most abundant in the 2.7‐10‐µm size class at most sites. Standard chemical fractionation of particles identified acid‐hydrolyzable P as the most abundant species of particulate P, with little reactive or refractory organic P. Sequential chemical extraction revealed that about 65% of total particulate P was microbial, while about 25% was associated with humic and fulvic organic matter. The size and chemical fractionation information suggested that P‐rich particles mostly consisted of suspended bacteria. Suspended particles in Everglades wetlands were small in size and had low concentrations, yet they stored a large proportion of surface‐water P in intermediately reactive forms, but they held little N.

Florida↗

The oxygen-18 isotope approach for measuring aquatic metabolism in high-productivity waters

We examined the utility of δ 18 O 2 measurements in estimating gross primary production (P), community respiration (R), and net metabolism (P : R) through diel cycles in a productive agricultural stream located in the midwestern U.S.A. Large diel swings in O 2 (±200 µmol L −1 ) were accompanied by large diel variation in δ 18 O 2 (±10‰). Simultaneous gas transfer measurements and laboratory‐derived isotopic fractionation factors for O 2 during respiration (α r ) were used in conjunction with the diel monitoring of O 2 and δ 18 O 2 to calculate P, R, and P :R using three independent isotope‐based methods. These estimates were compared to each other and against the traditional “open‐channel diel O 2 ‐change” technique that lacked δ 18 O 2 . A principal advantage of the δ 18 O 2 measurements was quantification of diel variation in R, which increased by up to 30% during the day, and the diel pattern in R was variable and not necessarily predictable from assumed temperature effects on R. The P, R, and P :R estimates calculated using the isotope‐based approaches showed high sensitivity to the assumed system fractionation factor (α r ). The optimum modeled ar values (0.986‐0.989) were roughly consistent with the laboratory‐derived values, but larger (i.e., less fractionation) than α r values typically reported for enzyme‐limited respiration in open water environments. Because of large diel variation in O 2 , P :R could not be estimated by directly applying the typical steady‐state solution to the O 2 and 18 O‐O 2 mass balance equations in the absence of gas transfer data. Instead, our results indicate that a modified steady‐state solution (the daily mean value approach) could be used with time‐averaged O 2 and δ 18 O 2 measurements to calculate P :R independent of gas transfer. This approach was applicable under specifically defined, net heterotrophic conditions. The diel cycle of increasing daytime R and decreasing nighttime R was only partially explained by temperature variation, but could be consistent with the diel production/consumption of labile dissolved organic carbon from photosynthesis.

Limnology and Oceanography↗

Nitrogen biogeochemistry of submarine groundwater discharge

To investigate the role of the seepage zone in transport, chemical speciation, and attenuation of nitrogen loads carried by submarine groundwater discharge, we collected nearshore groundwater samples (n = 328) and examined the distribution and isotopic signature (δ 15 N) of nitrate and ammonium. In addition, we estimated nutrient fluxes from terrestrial and marine groundwater sources. We discuss our results in the context of three aquifer zones: a fresh groundwater zone, a shallow salinity transition zone (STZ), and a deep STZ. Groundwater plumes containing nitrate and ammonium occurred in the freshwater zone, whereas the deep STZ carried almost exclusively ammonium. The distributions of redox-cycled elements were consistent with theoretical thermodynamic stability of chemical species, with sharp interfaces between water masses of distinct oxidation : reduction potential, suggesting that microbial transformations of nitrogen were rapid relative to dispersive mixing. In limited locations in which overlap occurs between distribution of nitrate with that of ammonium and dissolved Fe 2+ , changes in concentration and in δ 15 N suggest loss of all species. Concurrent removal of NO 3 − and NH 4 + , both in freshwater and the deep STZ, might occur through a range of mechanisms, including heterotrophic or autotrophic denitrification, coupled nitrfication : denitrification, anammox, or Mn oxidation of NH 4 + . Loss of nitrogen was not apparent in the shallow STZ, perhaps because of short water residence time. Despite organic Cpoor conditions, the nearshore aquifer and subterranean estuary are biogeochemically active zones, where attenuation of N loads can occur. Extent of attenuation is controlled by the degree of mixing of biogeochemically dissimilar water masses, highlighting the critical role of hydrogeology in N biogeochemistry. Mixing is related in part to thinning of the freshwater lens before discharge and to dispersion at the fresh : saline groundwater interface, features common to all submarine groundwater discharge zones.

Limnology and Oceanography↗

Why are diverse relationships observed between phytoplankton biomass and transport time?

Transport time scales such as flushing time and residence time are often used to explain variability in phytoplankton biomass. In many cases, empirical data are consistent with a positive phytoplankton‐transport time relationship (i.e., phytoplankton biomass increases as transport time increases). However, negative relationships, varying relationships, or no significant relationship may also be observed. We present a simple conceptual model, in both mathematical and graphical form, to help explain why phytoplankton may have a range of relationships with transport time, and we apply it to several real systems. The phytoplankton growth‐loss balance determines whether phytoplankton biomass increases with, decreases with, or is insensitive to transport time. If algal growth is faster than loss (e.g., grazing, sedimentation), then phytoplankton biomass increases with increasing transport time. If loss is faster than growth, phytoplankton biomass decreases with increasing transport time. If growth and loss are approximately balanced, then phytoplankton biomass is relatively insensitive to transport time. In analyses of several systems, portions of an individual system, or time periods, apparent insensitivity of phytoplankton biomass to changes in transport time could arise due to the superposition of cases with different phytoplankton‐transport time relationships. Thus, in order to understand or predict responses of phytoplankton biomass to changes in transport time, the relative rates of algal growth and loss must be known.

Limnology and Oceanography↗

Benthic biogeochemical cycling, nutrient stoichiometry, and carbon and nitrogen mass balances in a eutrophic freshwater bay

Green Bay, while representing only ~7% of the surface area and ~1.4% of the volume of Lake Michigan, contains one‐third of the watershed of the lake, and receives approximately one‐third of the total nutrient loading to the Lake Michigan basin, largely from the Fox River at the southern end of the bay. With a history of eutrophic conditions dating back nearly a century, the southern portion of the bay behaves as an efficient nutrient and sediment trap, sequestering much of the annual carbon and nitrogen input within sediments accumulating at up to 1 cm per year. Depositional fluxes of organic matter varied from ~0.1 mol C m22 yr21 to >10 mol C m −2 yr −1 and were both fairly uniform in stoichiometric composition and relatively labile. Estimates of benthic recycling derived from pore‐water concentration gradients, whole‐sediment incubation experiments, and deposition‐burial models of early diagenesis yielded an estimated 40% of the carbon and 50% of the nitrogen recycled back into the overlying water. Remineralization was relatively rapid with ~50% of the carbon remineralized within ~15 yr of deposition, and a mean residence time for metabolizable carbon and nitrogen in the sediments of 20 yr. On average, organic carbon regeneration occurred as 75% CO 2 , 15% CH 4 , and 10% dissolved organic carbon (DOC). Carbon and nitrogen budgets for the southern bay were based upon direct measurements of inputs and burial and upon estimates of export and production derived stoichiometrically from a coupled phosphorus budget. Loadings of organic carbon from rivers were ~3.7 mol m −2 yr −1 , 80% in the form of DOC and 20% as particulate organic carbon. These inputs were lost through export to northern Green Bay and Lake Michigan (39%), through sediment burial (26%), and net CO 2 release to the atmosphere (35%). Total carbon input, including new production, was 4.54 mol m −2 yr −1 , equivalent to ~10% of the gross annual primary production. Nitrogen budget terms were less well quantified, with nitrogen export ~54% of total inputs and burial ~24%, leaving an unquantified residual loss term in the nitrogen budget of ~22%.

Green Bay↗

Hydrodynamic control of phytoplankton loss to the benthos in an estuarine environment

Field experiments were undertaken to measure the influence of hydrodynamics on the removal of phytoplankton by benthic grazers in Suisun Slough, North San Francisco Bay. Chlorophyll a concentration boundary layers were found over beds inhabited by the active suspension feeders Corbula amurensis and Corophium alienense and the passive suspension feeders Marenzellaria viridis and Laonome sp. Benthic losses of phytoplankton were estimated via both the control volume and the vertical flux approach, in which chlorophyll a concentration was used as a proxy for phytoplankton biomass. The rate of phytoplankton loss to the bed was positively correlated to the bed shear stress. The maximum rate of phytoplankton loss to the bed was five times larger than estimated by laboratory-derived pumping rates for the active suspension feeders. Reasons for this discrepancy are explored including a physical mechanism whereby phytoplankton is entrained in a near-bed fluff layer where aggregation is mediated by the presence of mucus produced by the infaunal community.

California↗

Hydrologic control of nitrogen removal, storage, and export in a mountain stream

Nutrient cycling and export in streams and rivers should vary with flow regime, yet most studies of stream nutrient transformation do not include hydrologic variability. We used a stable isotope tracer of nitrogen ( 15 N) to measure nitrate (NO 3 − ) uptake, storage, and export in a mountain stream, Spring Creek, Idaho, U.S.A. We conducted two tracer tests of 2‐week duration during snowmelt and baseflow. Dissolved and particulate forms of 15 N were monitored over three seasons to test the hypothesis that stream N cycling would be dominated by export during floods, and storage during low flow. Floods exported more N than during baseflow conditions; however, snowmelt floods had higher than expected demand for NO 3 − because of hyporheic exchange. residence times of benthic N during both tracer tests were longer than 100 d for ephemeral pools such as benthic algae and wood biofilms. Residence times were much longer in fine detritus, insects, and the particulate N from the hyporheic zone, showing that assimilation and hydrologic storage can be important mechanisms for retaining particulate N. Of the tracer N stored in the stream, the primary form of export was via seston during periods of high flows, produced by summer rainstorms or spring snowmelt the following year. Spring Creek is not necessarily a conduit for nutrients during high flow; hydrologic exchange between the stream and its valley represents an important storage mechanism.

Idaho↗

The role of effective discharge in the ocean delivery of particulate organic carbon by small, mountainous river systems

Recent research has shown that small, mountainous river systems (SMRS) account for a significant fraction of the global flux of sediment and particulate organic carbon (POC) to the ocean. The enormous number of SMRS precludes intensive studies of the sort conducted on large systems, necessitating development of a conceptual framework that permits cross‐system comparison and scaling up. Herein, we introduce the geomorphic concept of effective discharge to the problem of source‐to‐sink POC transport. This idea recognizes that transport effectiveness is the product of discharge frequency and magnitude, wherein the latter is quantified as a power‐law relationship between discharge and load (the ‚rating curve’). An analytical solution for effective discharge ( Q e ) identifies two key variables: the standard deviation of the natural logarithm of discharge (Σ q ), and the rating exponent of constituent i ( b i ). Data from selected SMRS are used to show that for a given river Q e ‐POC , Q e ‐sediment, Q e for different POC constituents (e.g., POC fossil vs. POC modern ) differs in predictable ways, and Q e for a particular constituent can vary seasonally. When coupled with the idea that discharge peaks of small rivers may be coincident with specific oceanic conditions (e.g., large waves, wind from a certain direction) that determine dispersal and burial, these findings have potentially important implications for POC fate on continental margins. Future studies of POC transport in SMRS should exploit the conceptual framework provided herein and seek to identify how constituent‐specific effective discharges vary between rivers and respond to perturbations.

Limnology and Oceanography↗

Effects of simultaneous climate change and geomorphic evolution on thermal characteristics of a shallow Alaskan lake

We used a hydrodynamics model to assess the consequences of climate warming and contemporary geomorphic evolution for thermal conditions in a large, shallow Alaskan lake. We evaluated the effects of both known climate and landscape change, including rapid outlet erosion and migration of the principal inlet stream, over the past 50 yr as well as future scenarios of geomorphic restoration. Compared to effects of air temperature during the past 50 yr, lake thermal properties showed little sensitivity to substantial (~60%) loss of lake volume, as the lake maximum depth declined from 6 m to 4 m driven by outlet erosion. The direction and magnitude of future lake thermal responses will be driven largely by the extent of inlet stream migration when it occurs simultaneously with outlet erosion. Maintaining connectivity with inlet streams had substantial effects on buffering lake thermal responses to warming climate. Failing to account for changing rates and types of geomorphic processes under continuing climate change may misidentify the primary drivers of lake thermal responses and reduce our ability to understand the consequences for aquatic organisms.

Alaska↗

An experimental analysis of harmful algae-zooplankton interactions and the ultimate defense

W We examined effects of the invasive, toxigenic haptophyte Prymnesium parvum on grazing rates, feeding behaviors, and life‐history characteristics of clonal lineages of three daphniid zooplankton species. Grazing experiments revealed similar clearance rates for P. parvum and a common green alga. Behavioral observations revealed no significant effects of P. parvum on daphniid feeding behaviors after 30 min, but major declines in appendage beat rates after 1 h. Chronic exposure (10 d) to P. parvum resulted in severe reductions in daphniid growth rates, age at first reproduction, fecundity, and survivorship at densities as low as 7750 cells mL −1 . Thus, in addition to direct fish mortality during P. parvum blooms of 50,000–200,000 cells mL −1 , the entire food web of an invaded system may be subjected to potentially severe negative consequences even at nonbloom densities of P. parvum .

Limnology and Oceanography↗

Evaluation of groundwater discharge into small lakes based on the temporal distribution of radon-222

In order to evaluate groundwater discharge into small lakes we constructed a model that is based on the budget of 222 Rn (radon, t 1/2 =3.8 d) as a tracer. The main assumptions in our model are that the lake's waters are well‐mixed horizontally and vertically; the only significant 222 Rn source is via groundwater discharge; and the only losses are due to decay and atmospheric evasion. In order to evaluate the groundwater‐derived 222 Rn flux, we monitored the 222 Rn concentration in lake water over periods long enough (usually 1–3 d) to observe changes likely caused by variations in atmospheric exchange (primarily a function of wind speed and temperature). We then attempt to reproduce the observed record by accounting for decay and atmospheric losses and by estimating the total 222 Rn input flux using an iterative approach. Our methodology was tested in two lakes in central Florida: one of which is thought to have significant groundwater inputs (Lake Haines) and another that is known not to have any groundwater inflows but requires daily groundwater augmentation from a deep aquifer (Round Lake). Model results were consistent with independent seepage meter data at both Lake Haines (positive seepage of ∼ 1.6 × 10 4 m 3 d −1 in Mar 2008) and at Round Lake (no net groundwater seepage)

Limnology and Oceanography↗