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States and rates: Complementary approaches to developing flow‐ecology relationships

In recognition of the influence of flow on riverine habitats and organisms, stream ecologists have devoted considerable effort to the development of quantitative predictive relationships describing ecological responses to flow variability, i.e. flow‐ecology relationships. Methods used to generate flow‐ecology relationships can be thought of as a continuum bookended by pure states approaches on one end and by rates approaches on the other. In pure states approaches, the ecological response is a snapshot of a condition or property (i.e. a state) derived from a single measurement in time. In contrast, ecological responses in rates approaches reflect temporal change (i.e. a rate) and are thus reliant on repeated measurements made over time. Here, we elaborate on the fundamental characteristics of different approaches (pure states, rates and an intermediate approach we call repeated states) for generating flow‐ecology relationships, examine how commonly the different approaches are used in the flow‐ecology literature, conduct an independent analysis to illustrate the different outcomes achieved by applying repeated‐states and rates approaches using a dataset for stream fish diversity in relation to flow magnitude, and identify some of the different ways ecologists are applying rates approaches in flow ecology. Our literature review revealed that repeated‐states approaches (53% of reviewed studies) were used far more commonly than either pure states (19%) or rates (12%) approaches to generate flow‐ecology relationships. The remaining hybrid studies (17%) used both state and rate responses, and thus also relied on repeated measurements over time. Despite frequent collection of data suitable for rates approaches, flow‐ecology relationships have generally been developed using states approaches that relate changes in ecological states to different long‐term average flow conditions, rather than to specific flow sequences over much shorter time intervals. Such flow‐ecology relationships cannot generate temporally specific predictions of ecological responses to changing flow conditions (i.e. the expected change in state following a specific flow sequence), nor can they describe demographic processes underlying observed changes. While there are different scenarios in which a pure or repeated‐states approach would be useful, more frequent use of rates approaches would increase our ability to test flow‐ecology hypotheses and our mechanistic understanding of flow‐ecology relationships.

Freshwater Biology

Literature review and database of relations between salinity and aquatic biota: Applications to Bowdoin National Wildlife Refuge, Montana

Long-term accumulation of salts in wetlands at Bowdoin National Wildlife Refuge (NWR), Mont., has raised concern among wetland managers that increasing salinity may threaten plant and invertebrate communities that provide important habitat and food resources for migratory waterfowl. Currently, the U.S. Fish and Wildlife Service (USFWS) is evaluating various water management strategies to help maintain suitable ranges of salinity to sustain plant and invertebrate resources of importance to wildlife. To support this evaluation, the USFWS requested that the U.S. Geological Survey (USGS) provide information on salinity ranges of water and soil for common plants and invertebrates on Bowdoin NWR lands. To address this need, we conducted a search of the literature on occurrences of plants and invertebrates in relation to salinity and pH of the water and soil. The compiled literature was used to (1) provide a general overview of salinity concepts, (2) document published tolerances and adaptations of biota to salinity, (3) develop databases that the USFWS can use to summarize the range of reported salinity values associated with plant and invertebrate taxa, and (4) perform database summaries that describe reported salinity ranges associated with plants and invertebrates at Bowdoin NWR. The purpose of this report is to synthesize information to facilitate a better understanding of the ecological relations between salinity and flora and fauna when developing wetland management strategies. A primary focus of this report is to provide information to help evaluate and address salinity issues at Bowdoin NWR; however, the accompanying databases, as well as concepts and information discussed, are applicable to other areas or refuges. The accompanying databases include salinity values reported for 411 plant taxa and 330 invertebrate taxa. The databases are available in Microsoft Excel version 2007 (http://pubs.usgs.gov/sir/2009/5098/downloads/databases_21april2009.xls) and contain 27 data fields that include variables such as taxonomic identification, values for salinity and pH, wetland classification, location of study, and source of data. The databases are not exhaustive of the literature and are biased toward wetland habitats located in the glaciated North-Central United States; however, the databases do encompass a diversity of biota commonly found in brackish and freshwater inland wetland habitats.

Montana

Unifying research on the fragmentation of terrestrial and aquatic habitats: patches, connectivity and the matrix in riverscapes

While there is an increasing emphasis in terrestrial ecology on determining the influence of the area that surrounds habitat patches (the landscape ‘matrix’) relative to the characteristics of the patches themselves, research on these aspects in running waters is still rather underrepresented. Here we outline conceptual foundations of matrix ecology for stream and river ecosystems (‘riverscapes’). We discuss how a hierarchical, patch-based perspective is necessary for the delineation of habitat patches and the surrounding matrix, through which we may identify two classes of habitat edges in riverscapes (i.e. edges between the terrestrial–aquatic interface and edges within streams). Under this conceptual framework, we discuss the role of the matrix in influencing between-patch movement, and resource quality and quantity within and among habitat patches in riverscapes. We also review types of empirical and modelling approaches which may advance our understanding of fragmentation effects in these systems. We identify five key challenges for understanding fragmentation and matrix effects more completely: (i) defining populations and their status (i.e. quantifying the demographic contribution of habitat patches to metapopulation dynamics), (ii) scaling from metapopulations to metacommunities (particularly searching for generalities in species responses to landscape heterogeneity), (iii) scaling from metacommunities to metaecosystems (i.e. exploring the interactive role of the terrestrial–aquatic and within-stream matrix effects on the flow of material and energy at the network scale), (iv) understanding temporal dynamics in matrix permeability and (v) revealing the utility of different patch and matrix representations for modelling connectivity relationships. Fragmentation of habitats is a critical issue in the conservation and management of stream networks across spatial scales. Although the effects of individual barriers (e.g. dams) are well documented, we argue that a more comprehensive patch–matrix landscape model will improve our understanding of fragmentation effects and improve management in riverscapes.

Freshwater Biology

The Mekong Fish Network: expanding the capacity of the people and institutions of the Mekong River Basin to share information and conduct standardized fisheries monitoring

The Mekong River is one of the most biologically diverse rivers in the world, and it supports the most productive freshwater fisheries in the world. Millions of people in the Lower Mekong River Basin (LMB) countries of the Union of Myanmar (Burma), Lao People’s Democratic Republic, the Kingdom of Thailand, the Kingdom of Cambodia, and the Socialist Republic of Vietnam rely on the fisheries of the basin to provide a source of protein. The Mekong Fish Network Workshop was convened in Phnom Penh, Cambodia, in February 2012 to discuss the potential for coordinating fisheries monitoring among nations and the utility of establishing standard methods for short- and long-term monitoring and data sharing throughout the LMB. The concept for this network developed out of a frequently cited need for fisheries researchers in the LMB to share their knowledge with other scientists and decisionmakers. A fish monitoring network could be a valuable forum for researchers to exchange ideas, store data, or access general information regarding fisheries studies in the LMB region. At the workshop, representatives from governments, nongovernmental organizations, and universities, as well as participating foreign technical experts, cited a great need for more international cooperation and technical support among them. Given the limited staff and resources of many institutions in the LMB, the success of the proposed network would depend on whether it could offer tools that would provide benefits to network participants. A potential tool discussed at the workshop was a user-friendly, Web-accessible portal and database that could help streamline data entry and storage at the institutional level, as well as facilitate communication and data sharing among institutions. The workshop provided a consensus to establish pilot standardized data collection and database efforts that will be further reviewed by the workshop participants. Overall, workshop participants agreed that this is the type of support that is greatly needed to answer their most pressing questions and to enable local researchers and resource managers to monitor and sustain the valuable and diverse aquatic life of the Mekong River.

Mekong River

Using biological responses to monitor freshwater post-spill conditions over 3 years in Blacktail Creek, North Dakota, USA

A pipeline carrying unconventional oil and gas (OG) wastewater spilled approximately 11 million liters of wastewater into Blacktail Creek, North Dakota, USA. Flow of the mix of stream water and wastewater down the channel resulted in storage of contaminants in the hyporheic zone and along the banks, providing a long-term source of wastewater constituents to the stream. A multi-level investigation was used to assess the potential effects of oil and brine spills on aquatic life. In this study, we used a combination of experiments using a native fish species, Fathead Minnow ( Pimephales promelas ), field sampling of the microbial community structure, and measures of estrogenicity. The fish investigation included in situ experiments and experiments with collected site water. Estrogenicity was measured in collected site water samples, and microbial community analyses were conducted on collected sediments. During the initial post-spill investigation, February 2015, performing in situ fish bioassays was impossible because of ice conditions. However, microbial community (e.g., the presence of members of the Halomonadaceae, a family that is indicative of elevated salinity) and estrogenicity differences were compared to reference sites and point to early biological effects of the spill. We noted water column effects on in situ fish survival 6 months post-spill during June 2015. At that time, total dissolved ammonium (sum of ammonium and ammonia, TAN) was 4.41 mg NH 4 /L with an associated NH 3 of 1.09 mg/L, a concentration greater than the water quality criteria established to protect aquatic life. Biological measurements in the sediment defined early and long-lasting effects of the spill on aquatic resources. The microbial community structure was affected during all sampling events. Therefore, sediment may act as a sink for constituents spilled and as such provide an indication of continued and cumulative effects post-spill. However, lack of later water column effects may reflect pulse hyporheic flow of ammonia from shallow ground water. Combining fish toxicological, microbial community structure and estrogenicity information provides a complete ecological investigation that defines potential influences of contaminants at organismal, population, and community levels. In general, in situ bioassays have implications for the individual survival and changes at the population level, microbial community structure defines potential changes at the community level, and estrogenicity measurements define changes at the individual and molecular level. By understanding effects at these various levels of biological organization, natural resource managers can interpret how a course of action, especially for remediation/restoration, might affect a larger group of organisms in the system. The current work also reviews potential effects of additional constituents defined during chemistry investigations on aquatic resources.

North Dakota

Research in thermal biology: Burning questions for coldwater stream fishes

With the increasing appreciation of global warming impacts on ecological systems, in addition to the myriad of land management effects on water quality, the number of literature citations dealing with the effects of water temperature on freshwater fish has escalated in the past decade. Given the many biological scales at which water temperature effects have been studied, and the growing need to integrate knowledge from multiple disciplines of thermal biology to fully protect beneficial uses, we held that a survey of the most promising recent developments and an expression of some of the remaining unanswered questions with significant management implications would best be approached collectively by a diverse research community. We have identified five specific topic areas of renewed research where new techniques and critical thought could benefit coldwater stream fishes (particularly salmonids): molecular, organism, population/species, community and ecosystem, and policy issues in water quality. Our hope is that information gained through examination of recent research fronts linking knowledge at various scales will prove useful in managing water quality at a basin level to protect fish populations and whole ecosystems. Standards of the past were based largely on incipient lethal and optimum growth rate temperatures for fish species, while future standards should consider all integrated thermal impacts to the organism and ecosystem. ?? Taylor and Francis Group, LLC.

Reviews in Fisheries Science

Southern Salish Sea Habitat Map Series: Admiralty Inlet

In 2010 the Environmental Protection Agency, Region 10 initiated the Puget Sound Scientific Studies and Technical Investigations Assistance Program, designed to support research in support of implementing the Puget Sound Action Agenda. The Action Agenda was created in response to Puget Sound having been designated as one of 28 estuaries of national significance under section 320 of the U.S. Clean Water Act, and its overall goal is to restore the Puget Sound Estuary's environment by 2020. The Southern Salish Sea Mapping Project was funded by the Assistance Program request for proposals process, which also supports a large number of coastal-zone- and ocean-management issues. The issues include the recommendations of the Marine Protected Areas Work Group to the Washington State Legislature (Van Cleve and others, 2009), which endorses a Puget Sound and coast-wide marine conservation needs assessment, gap analysis of existing Marine Protected Areas (MPA) and recommendations for action. This publication is the first of four U.S. Geological Survey Scientific Investigation Maps that make up the Southern Salish Sea Mapping Project. The remaining three map blocks to be published in the future, located south of Admiralty Inlet, are shown in figure 1. Puget Sound is a deep, fjord-type estuary covering an area of 2,330 km 2 in the Pacific Northwest region of the United States (fig. 1). It is connected to the ocean by the Strait of Juan de Fuca, a turbulent passage approximately 160 km in length and 22 km wide at its west end, expanding to over 40 km wide at its east end (Thomson, 1994). During the Pleistocene, the area was occupied several times by lobes of continental ice, resulting in a complex basin-fill of glacial and interglacial deposits that are locally as thick as 1100 m (Johnson and others, 2001). The last glaciation, called the Fraser glaciation, began after 28,800±740 14 C yr B.P. when ice started a slow expansion (Clague, 1981). At peak advance the westward Juan de Fuca lobe reached the edge of the continental shelf through the Juan de Fuca Strait shortly before 14,460±200 14 C yr B.P. (Herzer and Bornhold, 1982). The southward Puget lobe advanced to its terminal position in Puget Sound by around 14,150 14 C yr B.P. (Porter and Swanson, 1998). Ice retreated from its maximum to northern Whidbey Island by 13,650±350 14 C yr B.P. (Dethier and others, 1995). Retreating glaciers resulted in a thick sequence of ice-contact, glacial-marine sediment, and early post-glacial sediments (Linden and Schurrer, 1988). These deposits have experienced the effects of a marine transgression followed by regression, resulting in a sea-level several tens of meters lower than the present day (Linden and Schurrer, 1988). A second transgression brought sea level to about the present level by around 5,470±120 14 C yr B.P. (Clague and others, 1982) establishing the present oceanographic and geologic environment Puget Sound is separated into four interconnected basins; Whidbey, Central (Main), Hood Canal, and South (Thomson, 1994). The Whidbey, Central, and Hood Canal basins are the three main branches of the Puget Sound estuary and are separated from the Strait of Juan de Fuca by a double sill at Admiralty Inlet. The Admiralty Inlet map area includes the Inlet and a portion of the Whidbey Basin (fig. 1). The shallower South Basin is separated by a sill at Tacoma Narrows and is highly branched with numerous finger inlets. Flow within Puget Sound is dominated by tidal currents of as much as 1 m/s at Admiralty Inlet, reducing to approximately 0.5 m/s in the Central Basin (Lavelle and others, 1988). The lack of silt and clay-sized sediments in the Admiralty Inlet map area is likely a result of the strong currents (see Ground-Truth Studies for the Admiralty Inlet Map Area, sheet 3). The subtidal component of flow reaches approximately 0.1 m/s and is driven by density gradients arising from the contrast in salty ocean water at the entrance and freshwater inputs from stream flow (Lavelle and others, 1988). The total freshwater input to Puget Sound is approximately 3.4 x 10 6 m 3 /day, primarily from the Skagit River (Cannon, 1983). The subtidal circulation mostly consists of a two-layered flow in the basins with fresher water exiting at the surface and saltier water entering at depth (Ebbesmeyer and Cannon, 2001). In general, surface waters flow north and deeper waters flow south; variations arise from wind effects that can drive a surface current in the same direction as the wind, and a baroclinic response in the lower layer to about 100-m depth (Matsuura and Cannon, 1997). Oceanographic properties are influenced by temporal forcing parameters such as reduced stream flow during the 2000-01 drought that increased surface salinity and decreased differences between surface and bottom waters (Newton and others, 2003). On offshore seismic-reflection profiles, Pleistocene strata (excluding latest Pleistocene glacial and post-glacial deposits) form a distinct seismic unit, bounded below by pre-Tertiary or Tertiary basement and above by typically flat-lying latest Pleistocene to Holocene deposits that fill in erosional or depositional relief (Johnson and others, 2001). Cores from central Puget Sound have accumulation rates that range from 85 to 1200 mg/cm 2 /yr, or 0.12 to 2.4 cm/yr; the highest accumulation rates are near the southern end of central Puget Sound (Carpenter and others, 1985). Carpenter and others (1985) un-weighted arithmetic mean of accumulation rates for central Puget Sound deeper stations is 480±340 (± one standard deviation) mg/cm 2 /yr. Lavelle and others (1985) also found rates as high as 1200 mg/cm 2 /yr over the past approximately 70 years in cores in the Central Basin off of and north and south of Elliott Bay. Puget Sound basin rates are comparable to rates in midshelf silt deposits on the Washington coast north of the Columbia River (Nittrouer and others, 1979). The deep subtidal (in other words, below SCUBA depths) habitats of Puget Sound are relatively poorly known. A few subtidal surveys exist for several habitat types from the 1960s and 1970s (reviewed in Dethier, 1990), using grab and box core data. The Dethier (1990) review divides habitat up into Coast and Marine Ecological Classification Standard (CMECS) substrate, water column energy, and depth zones but does not attempt to map these habitats, rather it is an inventory of habitats found in the area and the flora and fauna associated with each habitat. The approach of the Southern Salish Sea Mapping project is to create highly detailed seafloor maps through collection, integration, interpretation, and visualization of swath sonar data (the undersea equivalent of satellite remote-sensing data in terrestrial mapping), acoustic backscatter, seafloor video, seafloor photography, and bottom-sediment sampling data. This approach is based in part on methods presented and data collection and product needs identified at the Washington State Seafloor Mapping Workshop (Washington State Seafloor Mapping Workshop Steering Committee, 2008), attended by coastal and marine managers and scientists. The map products display seafloor geomorphology and substrate, and identify potential marine benthic habitats. It is emphasized that the more interpretive habitat and geology maps rely on the integration of multiple, new high-resolution datasets and that mapping at small scales would not be possible without such data. Oceanographic current and wave data is not included in this analysis, however, the accompanying geographic information system (GIS) data set is designed and intended to be combined with oceanographic and biologic data sets assembled by others in the future and some of the GIS data has already been incorporated in the unpublished Nature Conservancy Benthic Habitats of Puget Sound database. This publication includes four map sheets, explanatory text, and a descriptive pamphlet. Each map sheet is published as a portable document format (PDF) file. ESRI ArcGIS compatible geotiffs (for example, bathymetry) and shapefiles (for example video observation points) will be available for download in the data catalog associated with this publication (Cochrane, 2015). An ArcGIS Project File with the symbology used to generate the map sheets is also provided. For those who do not own the full suite of ESRI GIS and mapping software, the data can be read using ESRI ArcReader, a free viewer that is available at http://www.esri.com/software/arcgis/arcreader/index.html .

Washington

Histological assessment of organs in sexually mature and post-spawning steelhead trout and insights into iteroparity

Steelhead trout ( Oncorhynchus mykiss ) are anadromous and iteroparous, but repeat-spawning rates are generally low. Like other anadromous salmonids, steelhead trout fast during freshwater spawning migrations, but little is known about the changes that occur in vital organs and tissues. We hypothesized that fish capable of repeat-spawning would not undergo the same irreversible degeneration and cellular necrosis documented in semelparous salmon. Using Snake River steelhead trout as a model we used histological analysis to assess the cellular architecture in the pyloric stomach, ovary, liver, and spleen in sexually mature and kelt steelhead trout. We observed 38 % of emigrating kelts with food or fecal material in the gastrointestinal tract. Evidence of feeding was more likely in good condition kelts, and feeding was associated with a significant renewal of villi in the pyloric stomach. No vitellogenic oocytes were observed in sections of kelt ovaries, but perinucleolar and early/late stage cortical alveolus oocytes were present suggesting iteroparity was possible. We documented a negative correlation between the quantity of perinucleolar oocytes in ovarian tissues and fork length of kelts suggesting that larger steelhead trout may invest more into a single spawning event. Liver and spleen tissues of both mature and kelt steelhead trout had minimal cellular necroses. Our findings indicate that the physiological processes causing rapid senescence and death in semelparous salmon are not evident in steelhead trout, and recovery begins in fresh water. Future management efforts to increase iteroparity in steelhead trout and Atlantic salmon must consider the physiological processes that influence post-spawning recovery.

Reviews in Fish Biology and Fisheries

Unified 200 kyr paleohydrologic history of the Southern Great Basin: Death Valley, Searles Valley, Owens Valley and the Devils Hole cave

We present a hydroclimate synthesis of the southern Great Basin over the last two glacial-interglacial cycles focused on paleolakes in Death Valley (core DV93-1), Searles Valley (core SLAPP-SRLS17), Owens Valley (core OL92), and the Devils Hole cave. There is close agreement between the occurrence of lakes in Death Valley and the height of the water table in the Devils Hole (50 km east of Death Valley) during the last 200 kyr. Death Valley and Devils Hole have adjacent, partly overlapping, drainage areas and most likely did over the last 200 kyr. When the water table in the Devils Hole was above the threshold level of ∼5 m higher than the modern, permanent lakes existed in Death Valley. At water table elevations less than 5 m above the modern, ephemeral lakes, saline pans, and mudflats occurred in Death Valley. The close temporal agreement between inferred paleoenvironments from the sediments in the Death Valley core and the paleowater table elevation in Devils Hole suggests a common forcing and provides insight into climate variability in the southwestern United States over the last 200 kyr. Owens Valley and Searles Valley, which derived inflow waters from the Sierra Nevada via the Owens River, contain paleohydrologic records which match those from Death Valley and the Devils Hole in terms of timing and direction of water availability over the last 200 kyr, indicating a similar paleohydrologic history for the entire southern Great Basin region. Near the end of Marine Oxygen Isotope Stage 6 (MIS 6), 140 ka - 130 ka, Lake Manly in Death Valley became shallow and hypersaline, and ultimately dried up at 127.1 ka ±4.3 ka. The transition from glacial to interglacial vegetation, which involved the loss of Juniperus pollen and an increase in Quercus (oak) pollen, occurred in Death Valley core DV93-1 at 131.3 ka ±4.0 ka. Following the glacial to interglacial pollen shift, a large alkaline lake formed in Death Valley. Similar conditions (freshwater, high productivity, and a mixed, deeply oxygenated water column indicated by biomarkers) existed in Searles Lake between 135.3 +2.7 / -2.9 ka and 130.1 +2.7 / -2.6 ka, also following the juniper-oak pollen transition. Sr isotopes in calcite and sulfate minerals (gypsum, glauberite, thenardite), and the rare occurrence of the sodium carbonate mineral northupite with a low 87 Sr/ 86 Sr ratio in core DV93-1, together with organic geochemical proxies from Searles core SLAPP-SRLS17, all suggest that at this time, late MIS 6 Lake Manly in Death Valley received alkaline water via spillover from Searles Valley into Death Valley through Panamint Valley. The hydrologic connection between Searles Valley, Panamint Valley, and Death Valley at Termination II (130 ka) is documented here for this system of pluvial lakes for the first time. The Devils Hole water table decreased to +6.5 m at 140.8 ka ±3.2 ka, rose briefly to +8 m at 137.6 ka ±0.5 ka, and then dropped 8 m by 120.36 ka ±0.45 ka, when it reached an elevation similar to the modern. The pluvial lakes in Death Valley and Searles Valley may have coincided with the rise of the Devils Hole water table at ∼137.6 ka ±0.5 ka years ago, although the age models for core DV93-1 and core SLAPP-SLRS17 during the end of MIS 6 carry large uncertainties.

Quaternary Science Reviews

A multi-decadal geochemical record from Rano Aroi (Easter Island/Rapa Nui): Implications for the environment, climate and humans during the last two millennia

The small and remote Easter Island (Rapa Nui) has a complex and still partially unknown history of human colonization and interactions with the environment. Previous research from sedimentary archives collected in the three freshwater bodies of Rapa Nui document dramatic environmental changes over the last two millennia. Yet, the characteristics of sediments and paleoenvironmental records are challenging to interpret, mainly due to poor temporal resolution, hiatuses and sediment mixing. In this study, we reconstruct past changes in lithogenic inputs, weathering processes, redox conditions, productivity and water levels in the Rano Aroi wetland over the last 2000 years through the determination of major, trace and rare earth elements in a new peat core collected in 2017. The chronology is based on 8 14 C AMS dates for the upper 1.5 m and provides decadal to multi-decadal resolution which is unprecedented for the island of Rapa Nui. The multielemental proxies depict seven distinct chronological phases marked by well-defined geochemical transitions. With only a few minor fluctuations, climate conditions were dry and the mire was mildly anoxic during the first millennium (0–1000 CE) to the arrival of the first Polynesians in Rapa Nui (800–1300 CE) and until ∼1400 CE, followed by wetter conditions afterwards. The record documents with unprecedented accuracy and resolution intense droughts occurring during the middle Little Ice Age between 1520 and 1710 CE, which may have been exacerbated by human activities and triggered dramatic cultural shifts. During the interval of first contact between the Rapanuis and Europeans, the climate changed to wetter conditions, followed by intense precipitations between 1790 and 1900 CE.

Quaternary Science Reviews

Evaluating effectiveness of restoration to address current stressors to riverine fish

River restoration programmes with the goal of conserving and rehabilitating inland fishes have a multi-decadal history, but evaluation and synthesis of past restoration actions have been limited by a lack of monitoring and reporting. Given that calls for both monitoring and systematic reviews of restoration have increased, we were interested in the influence that restoration has had on improving conditions for riverine fishes resulting from long-standing and increasingly prominent stressors. Our objectives were to (1) identify which stressors were targeted in river restoration efforts, (2) determine the proportion of published studies that articulate restoration goals and develop comparative monitoring designs for assessing the effects of restoration on fishes, and (3) conduct a meta-analysis to synthesise fish responses to restoration projects. We assessed restoration effectiveness for increasing fish density and richness from peer-reviewed papers published over the past decade using a global systematic review and meta-analysis. We found that restoration actions addressed major stressors primarily by improving in-stream habitat (37%), increasing in-stream longitudinal connectivity (26%) and increasing lateral floodplain connections (9%). Although 81% of studies had comparative monitoring designs (i.e., before/after and control/impact) and stated restoration goals, only 40% of those studies reported sufficient data to be included in the meta-analysis. Projects which increased in-stream connectivity had the largest positive effect size on fish density and richness compared to in-stream habitat improvements and increasing floodplain connections. Time since restoration and restoration size (i.e., geographical footprint) were not strong predictors of fish response effect sizes. Restoration effectiveness was highly variable among project types. Authors of studies included in the meta-analysis often identified spatial or temporal scale of monitoring, overriding catchment conditions, and recolonisation potential as sources of variability and effectiveness in restoration outcomes. Systematic reporting of these and other covariates may help guide processes in restoration evaluation and provide valuable research insights. Despite increased emphasis on monitoring, incomplete data reporting limited the number of studies that could be included for quantitative meta-analysis. Persistent emphasis on setting specific criteria (e.g., measurable outcomes of fish response) for restoration goals, project monitoring, data reporting, information sharing and collaborative projects is likely to continue to improve understanding of restoration effectiveness transferable to future endeavours. Our results can be used to support river restoration practitioners with evidence-based information to evaluate the cost–benefit ratio of competing restoration priorities, and inform restoration planning and implementation for riverine fish.

Freshwater Biology

Contrasting Common Era climate and hydrology sensitivities from paired lake sediment dinosterol hydrogen isotope records in the South Pacific Convergence Zone

Hydroclimate on ‘Uvea (Wallis et Futuna) is controlled by rainfall associated with the South Pacific Convergence Zone (SPCZ), the southern hemisphere's largest precipitation feature. To extend the short observational precipitation record, the hydrogen isotopic composition of the algal lipid biomarker dinosterol (δ 2 H dinosterol ) was measured in sediment cores from two volcanic crater lakes on ‘Uvea. The modern lakes differ morphologically and chemically but both contain freshwater within the photic zone, support phytoplankton communities inclusive of dinosterol-producing dinoflagellates, and experience identical climate conditions. δ 2 H dinosterol values track lake water isotope ratios, ultimately controlled in the tropics by precipitation amount and evaporative enrichment. However, in 88-m-deep Lac Lalolalo a steadily decreasing trend in sedimentary δ 2 H dinosterol values from −227‰ around year 988 CE to modern values as low as −303‰, suggests this lake's evolution from an active volcanic setting to the present system strongly influenced δ 2 H dinosterol values. Although current hydrology and water isotope systematics may now reflect precipitation and evaporation in this lake, the interaction between these processes and large changes in basin morphology, geochemistry, and hydrology obstruct the recovery of a climate signal from Lac Lalolalo's sedimentary δ 2 H dinosterol records. This work emphasizes the importance of site replication and the use of complementary climate reconstruction tools, especially when using molecular proxies that may be sensitive to more than one environmental parameter. Contrary to its neighbor, duplicate δ 2 H dinosterol records from 23-m-deep Lac Lanutavake varied between −277‰ and −297‰ and indicate slightly drier conditions during the time-period known as the Medieval Climate Anomaly (MCA, 950–1250 CE). The δ 2 H dinosterol signal in Lac Lanutavake was muted compared to published records from ‘Upolu (Samoa) and Efate (Vanuatu) indicating that ‘Uvea's location is not as sensitive to precipitation variability at sites farther from the SPCZ central axis. Lithogenic runoff proxies combined with δ 2 H dinosterol support the interpretation of a relatively dry MCA on ‘Uvea, ‘Upolu, and Efate, potentially due to less intense precipitation, a contracted, or a more zonally oriented SPCZ.

Efate Island, Lac Lalolalo, Lac Lanoto'o, Lac Lanu

A science and management partnership to restore coregonine diversity to the Laurentian Great Lakes

Similar to many freshwater ecosystems, the Laurentian Great Lakes of North America have undergone numerous anthropogenic stressors resulting in considerable loss of biodiversity and habitat. Among Great Lakes fishes, the coregonine sub-family has endured the most extensive declines, including extinction of several species ( Coregonus johannae, C. alpenae , and C. kiyi orientalis ) and at least 10 instances of local extirpations of other species ( C. nigripinnis, C. reighardi, C. zenithicus, C. hoyi , and C. artedi ) across all 5 lakes, much of which occurred prior to the 1960s owing to overfishing, interactions with non-indigenous species, and habitat loss. Despite these declines, no federal-, provincial-, or state-mandated actions were ever implemented to conserve coregonine diversity, potentially because so much of the coregonine declines occurred prior to the enactment of federal conservation legislation. Possible explanations for inaction since enactment of that legislation include insufficient data on biological vulnerability or threats, unresolved taxonomy, and limited support from the fishery management agencies and their stakeholders prior to the 2000s. In recent decades, however, several fishery management agencies have undertaken efforts to re-introduce coregonine diversity. These efforts helped lead to development of a science-based framework to restore coregonines that was universally endorsed by fishery managers representing eight U.S. states, four U.S. tribal organizations, and the province of ON, Canada, in May 2018. The basin-wide framework is based on principles of conservation biology and adaptive management. We describe details of its key steps, including planning, restoring, and evaluating, while also describing recent implementation efforts to develop methods, improve available resources, and enhance coordination across the basin. Although our paper describes a regional effort to restore native coregonines, our adaptive-management approach could be used by other multi-agency stakeholders seeking to conserve or restore native fishes.

Laurentian Great Lakes

Water-quality assessment of part of the upper Mississippi River Basin, Minnesota and Wisconsin — Pesticides in streams, streambed sediment, and ground water, 1974-94

Available data on pesticides in streams, streambed sediment, and ground water from Federal, state, and local agencies are reviewed for part of the Upper Mississippi River Basin study unit of the National Water-Quality Assessment Program. The analysis focuses on a smaller study area encompassing 19,500 square miles that includes the Upper Mississippi River Basin from Lake Pepin upstream to sampling stations on the Mississippi River near Royalton, Minnesota, and the Minnesota River near Jordan, Minnesota, and the entire drainage basins of the St. Croix, Vermillion, and Cannon Rivers. Assessment is generally restricted to two groups of pesticides the most frequently detected herbicides and organochlorine insecticides although pesticides rarely or never detected are noted. Herbicides, including alachlor, atrazine, cyanazine, or metolachlor, were detected in every stream sampled except the Kettle River. Streams draining row-crop areas had the most herbicide detections. Atrazine was the most widely detected herbicide, with detections in all streams sampled except the Kettle River. Concentrations of atrazine, metolachlor, and cyanazine were greatest in July and detectable most of the year at very low (parts-per-trillion) concentrations. The herbicides EPTC and trifluralin were never detected, although they were used in amounts equal to or greater than those detected, reflecting the fact that some herbicides are less persistent than others. A small urban stream draining part of the Lake Harriet Watershed in Minneapolis, Minnesota, contained substantial concentrations of pesticides as well. Eighty-five percent of runoff events sampled in this entirely urbanized watershed had detections of herbicides commonly used for residential purposes, and 43 percent of the events had detections of alachlor, atrazine, cyanazine, or metolachlor herbicides used predominantly for agriculture. Pesticide concentrations in urban runoff remained well above detection limits throughout the summer, indicating repeated applications of pesticides. Selected organochlorine insecticides, banned since the 1970's, still were detected in recent streambed-sediment samples. Three insecticides, 4,4'-DDT, heptachlor, and lindane, and their metabolites account for almost two-thirds of the organochlorine insecticides detected. Organochlorine insecticides were detected more frequently in streambed sediment than in streamwater. Detections in both phases were most frequent within or downstream of the Twin Cities metropolitan area, indicating that most of these insecticides originated from the Twin Cities metropolitan area. The most frequently detected herbicides in ground water were the same as those frequently detected in streams. Most detections were found in the sand and gravel aquifers underlying agricultural areas, including the Anoka Sand Plain and Bonanza Valley. Atrazine, deethylatrazine, and deisopropylatrazine were detected most frequently. Detection frequencies of atrazine were extremely variable among the various agencies, ranging from 0 to 66.7 percent, probably as a result of different sampling purposes, well locations, and detection levels. Atrazine and atrazine metabolites were the only pesticides detected in bedrock aquifers, with detections found mainly in the agriculture-dominated southeastern part of the study area where bedrock commonly outcrops near the surface. Thus, most detections of herbicides in ground water were found in environmental settings where ground water is vulnerable to contamination. Atrazine was the only pesticide that equaled or exceeded a maximum contaminant level (of 3.0 micrograms per liter) for drinking water. Two stream samples from a small urban watershed in Minneapolis had atrazine concentrations of 3.6 and 3.8 micrograms per liter, and one ground-water sample had a concentration of 3.0 micrograms per liter. Trace concentrations (less than 0.06 micrograms per liter) of the organochlorine insecticides chlordane, dieldrin, endrin, and heptachlor exceeded chronic freshwater-quality criteria in stream samples from the Mississippi, Minnesota, St. Croix, and Vemillion Rivers in 1981 and 1990.

Minnesota, Wisconsin

Pesticides in groundwater in the Anacostia River and Rock Creek watersheds in Washington, D.C., 2005 and 2008

The U.S. Geological Survey (USGS), in cooperation with the District Department of the Environment, conducted a groundwater-quality investigation to (a) determine the presence, concentrations, and distribution of selected pesticides in groundwater, and (b) assess the presence of pesticides in groundwater in relation to selected landscape, hydrogeologic, and groundwater-quality characteristics in the shallow groundwater underlying the Anacostia River and Rock Creek watersheds in Washington, D.C. With one exception, well depths were 100 feet or less below land surface. The USGS obtained or compiled ancillary data and information on land use (2001), subsurface sediments, and groundwater samples from 17 wells in the lower Anacostia River watershed from September through December 2005, and from 14 wells in the lower Anacostia River and lower Rock Creek watersheds from August through September 2008. Twenty-seven pesticide compounds, reflecting at least 19 different types of pesticides, were detected in the groundwater samples obtained in 2005 and 2008. No fungicides were detected. In relation to the pesticides detected, degradate compounds were as or more likely to be detected than applied (parent) compounds. The detected pesticides chiefly reflected herbicides commonly used in urban settings for non-specific weed control or insecticides used for nonspecific haustellate insects (insects with specialized mouthparts for sucking liquid) or termite-specific control. Detected pesticides included a combination of pesticides currently (2008) in use, banned or under highly restricted use, and some that had replaced the banned or restricted-use pesticides. The presence of banned and restricted-use pesticides illustrates their continued persistence and resistance to complete degradation in the environment. The presence of the replacement pesticides indicates the susceptibility of the surficial aquifer to contamination irrespective of the changes in the pesticides used. A preliminary review of the data collected in 2005 and 2008 indicated that differences in the surficial geology, land use (as a surrogate for pesticide use), and above-average precipitation for most of 2004 through 2008, as well as differences in the number and performance of USGS laboratory methods used, could have led to more pesticides detected in groundwater samples collected in 2008 than in groundwater samples collected in 2005. Thus, although data from both years of collection were used for interpretive analysis, emphasis was placed on the analysis of the data obtained in 2008. The presence of pesticides in shallow groundwater (less than approximately 100 ft (feet), or 30 m (meters), below land surface) indicated at least the upper surficial aquifer in Washington, D.C. was susceptible to contamination. One or more herbicides or insecticides were detected in groundwater samples collected from 50 percent of the shallow wells sampled in 2005, and from 62 percent of the shallow wells sampled in 2008. Differences among types of pesticides in shallow groundwater were apparent. The most frequently detected class of herbicides was the s-triazine compounds—atrazine, simazine, or prometon, or the atrazine-degradate compounds—2-chloro-4-ethylamino-6-amino-s-triazine (desethylatrazine or CIAT) and 2-chloro-4-isopropylamino-6-amino-s-triazine (hydroxyatrazine or OIET). The next most frequently detected classes of herbicides were the chloroacetanilides, including metolachlor and acetochlor, and the ureic herbicides, including diuron (and degradate, 3,4-dichloroaniline), fluometuron, metsulfuron methyl, sulfameturon, bromacil, and tebuthiuron. Insecticides also were detected, but less frequently than herbicides, with one or more insecticides present in groundwater samples from 38 percent of shallow wells sampled in 2008. Detected insecticides included parent or degradate compounds commonly used for either nonspecific or haustellate (sucking) insects, including chlorpyrifos and dichlorodiphenyldichloroethane (p,p’-DDD; a degradate of dichlorodiphenyltrichloroethane, DDT), and for termite control, including dieldrin, chlordane, heptachlor epoxide, (a degradate of heptachlor), fipronil, and the sulfone and sulfide degradates of fipronil. The concentrations of individual pesticides in shallow groundwater in both years were low. Maximum concentrations were no greater than a few tenths of a microgram per liter (μg/L); typical concentrations often were less than 0.1 μg/L. Multiple pesticides, however, commonly were present in groundwater. For example, in 2008, approximately 88 percent (7 of 8) of the wells that yielded a sample with at least one detectable pesticide contained five or more pesticides. The highest number of detections occurred in a groundwater sample from well WE Ca 32, which is located in a highly developed urban area; this sample contained 15 different pesticide residues. In relation to human and aquatic health, no pesticide concentration in either 2005 or 2008 exceeded Federal drinking-water standards. Groundwater samples from a few sites, however, contained levels of chiefly banned or restricted-use pesticides that exceeded other human-health and (or) aquatic-health guidelines. For example, concentrations of dieldrin in 2008 groundwater samples from three wells—WE Ca 32 (0.028 μg/L), WE Ba 11 (0.016 μg/L), and WW Ac 8 (0.014 μg/L)—fell within the range of concern for 2004 Federally approved non-regulatory USGS Health-Based Assessment benchmarks (0.002 to 0.2 μg/L), and exceeded earlier (1999) Federal criteria for drinking water (0.000052 μg/L). Other individual compounds whose concentrations exceeded 1999 Federal guidelines for samples from one or more of these three sites, or another site, included p,p’-DDD, dichlorodiphenyldichloroethylene (p,p’-DDE; another degradate of DDT), chlordane, and heptachlor epoxide. Pesticide concentrations in groundwater also were compared to three aquatic-health guidelines for freshwater (United States, Great Lakes, or Canada). One or more of these guidelines were exceeded in groundwater samples obtained in 2005 or 2008 for one or more of the compounds chlordane, dieldrin, heptachlor epoxide, p,p’-DDE, p,p’-DDD, and chlorpyrifos. The spatial distribution of pesticides in the shallow groundwater appeared to be related, in part, to land use, a surrogate for pesticide use. Although most of the wells sampled in this study are in parklands or other relatively open and accessible space, multiple pesticides most often were detected in 2008 groundwater samples collected from wells where a considerable percentage (in excess of 60 percent) of the land within a 500-m radius is developed space (residential, commercial, or other urban infrastructure). Insecticides were detected in wells surrounded by at least 50 percent, and most commonly by more than 80 percent, development. Well WE Ca 32, the site associated with the highest number of pesticide residues in groundwater (8 herbicides and 7 insecticides), is in a small residential park, where 99 percent of the surrounding land is well-maintained residential and commercial development. The vertical distribution of detected pesticides in shallow groundwater appeared to be related, in part, to depth below land surface, surficial-bedrock type, and differences in the chemistry of shallow groundwater. Pesticides were detected at relatively shallow depths in wells that may not have fully penetrated the shallow aquifer. For wells in which at least one pesticide was detected, the median depth below land surface to the top of the well screen was 5.8 m, and the maximum depth was 8.5 m. Among the types of surficial materials in which wells were completed—alluvium, terrace deposits, or Potomac Formation sub- or outcrops in the Coastal Plain Province, and saprolite or fractured bedrock (Laurel and Sykesville Formations) underlying saprolite in the Piedmont Province—no pesticides were detected in groundwater associated with wells completed in the alluvium or fractured bedrock. Detections occurred in some but not all wells completed in the other surficial materials. Overall, the pattern in occurrence appeared related to the local permeability of these sediments and groundwater chemistry. Groundwater with multiple pesticide detections tended to occur in permeable sediments (absent any appreciable overlying clay, silt, or clay-silt layers), in conjunction with other common urban contaminants (elevated chloride in excess of tens to hundreds of milligrams per liter (mg/L), and oxic, rather than reduced, groundwater as evidenced by elevated (in excess of 5 mg/L) concentrations of nitrate). The results of this investigation were compared to results from two other similar and recent studies on pesticide occurrence in the shallow aquifer. These included a study in the nearby Maryland and Delaware Coastal Plain Physiographic Province and one in the Maryland and Virginia Piedmont Physiographic Province. Results from these studies were similar to the current study in relation to (a) the types, frequencies, concentrations, and mixtures of pesticides detected; (b) compounds that exceeded human-and aquatic-health criteria; and (c) the occurrence and distribution of pesticides within the surficial aquifer in relation to depths, sediment types, and groundwater chemistries.

Washington D.C.

Spatially explicit habitat models for 28 fishes from the Upper Mississippi River System (AHAG 2.0)

Environmental management actions in the Upper Mississippi River System (UMRS) typically require pre-project assessments of predicted benefits under a range of project scenarios. The U.S. Army Corps of Engineers (USACE) now requires certified and peer-reviewed models to conduct these assessments. Previously, habitat benefits were estimated for fish communities in the UMRS using the Aquatic Habitat Appraisal Guide (AHAG v.1.0; AHAG from hereon). This spreadsheet-based model used a habitat suitability index (HSI) approach that drew heavily upon Habitat Evaluation Procedures (HEP; U.S. Fish and Wildlife Service, 1980) by the U.S. Fish and Wildlife Service (USFWS). The HSI approach requires developing species response curves for different environmental variables that seek to broadly represent habitat. The AHAG model uses species-specific response curves assembled from literature values, data from other ecosystems, or best professional judgment. A recent scientific review of the AHAG indicated that the model’s effectiveness is reduced by its dated approach to large river ecosystems, uncertainty regarding its data inputs and rationale for habitat-species response relationships, and lack of field validation (Abt Associates Inc., 2011). The reviewers made two major recommendations: (1) incorporate empirical data from the UMRS into defining the empirical response curves, and (2) conduct post-project biological evaluations to test pre-project benefits estimated by AHAG. Our objective was to address the first recommendation and generate updated response curves for AHAG using data from the Upper Mississippi River Restoration-Environmental Management Program (UMRR-EMP) Long Term Resource Monitoring Program (LTRMP) element. Fish community data have been collected by LTRMP (Gutreuter and others, 1995; Ratcliff and others, in press) for 20 years from 6 study reaches representing 1,930 kilometers of river and >140 species of fish. We modeled a subset of these data (28 different species; occurrences at sampling sites as observed in day electrofishing samples) using multiple logistic regression with presence/absence responses. Each species’ probability of occurrence, at each sample site, was modeled as a function of 17 environmental variables observed at each sample site by LTRMP standardized protocols. The modeling methods used (1) a forward-selection process to identify the most important predictors and their relative contributions to predictions; (2) partial methods on the predictor set to control variance inflation; and (3) diagnostics for LTRMP design elements that may influence model fits. Models were fit for 28 species, representing 3 habitat guilds (Lentic, Lotic, and Generalist). We intended to develop “systemic models” using data from all six LTRMP study reaches simultaneously; however, this proved impossible. Thus, we “regionalized” the models, creating two models for each species: “Upper Reach” models, using data from Pools 4, 8, and 13; and “Lower Reach” models, using data from Pool 26, the Open River Reach of the Mississippi River, and the La Grange reach of the Illinois River. A total of 56 models were attempted. For any given site-scale prediction, each model used data from the three LTRMP study reaches comprising the regional model to make predictions. For example, a site-scale prediction in Pool 8 was made using data from Pools 4, 8, and 13. This is the fundamental nature and trade-off of regionalizing these models for broad management application. Model fits were deemed “certifiably good” using the Hosmer and Lemeshow Goodness-of-Fit statistic (Hosmer and Lemeshow, 2000). This test post-partitions model predictions into 10 groups and conducts inferential tests on correspondences between observed and expected probability of occurrence across all partitions, under Chi-square distributional assumptions. This permits an inferential test of how well the models fit and a tool for reporting when they did not (and perhaps why). Our goal was to develop regionalized models, and to assess and describe circumstances when a good fit was not possible. Seven fish species composed the Lentic guild. Good fits were achieved for six Upper Reach models. In the Lower Reach, no model produced good fits for the Lentic guild. This was due to (1) lentic species being much less prominent in the Lower Reach study areas, and (2) those that do express greater prominence principally do so only in the La Grange reach of the Illinois River. Thus, developing Lower Reach models for Lentic species will require parsing La Grange from the other two Lower Reach study areas and fitting separate models. We did not do that as part of this study, but it could be done at a later time. Nine species comprised the Lotic guild. Good fits were achieved for seven Upper Reach models and six Lower Reach models. Four species had good fits for both regions (flathead catfish, blue sucker, sauger, and shorthead redhorse). Three species showed zoogeographic zonation, with a good model fit in one of the regions, but not in the region in which they were absent or rarely occurred (blue catfish, rock bass, and skipjack herring). Twelve species comprised the Generalist guild. Good fits were achieved for five Upper Reach models and eight Lower Reach models. Six species had good fits for both regions (brook silverside, emerald shiner, freshwater drum, logperch, longnose gar, and white bass). Two species showed zoogeographic zonation, with a good model fit in one of the regions, but not in the region in which they were absent or rarely occurred (red shiner and blackstripe topminnow). Poorly fit models were almost always due to the diagnostic variable “field station,” a surrogate for river mile. In these circumstances, the residuals for “field station” were non-randomly distributed and often strongly ordered. This indicates either fitting “pool scale” models for these species and regions, or explicitly model covariances between “field station” and the other predictors within the existing modeling framework. Further efforts on these models should seek to resolve these issues using one of these two approaches. In total, nine species, representing two of the three guilds (Lotic and Generalist), produced well-fit models for both regions. These nine species should comprise the basis for AHAG 2.0. Additional work, likely requiring downscaling of the regional models to pool-scale models, will be needed to incorporate additional species. Alternately, a regionalized AHAG could be comprised of those species, per region, that achieved well-fit models. The number of species and the composition of the regional species pools will differ among regions as a consequence. Each of these alternatives has both pros and cons, and managers are encouraged to consider them fully before further advancing this approach to modeling multi-species habitat suitability.

Upper Mississippi River System

Crocodylus acutus (American Crocodile). Long distance juvenile movement

Crocodylus acutus (American Crocodile) is the most widely distributed New World crocodilian species with its range extending from Peru in the south to the southern tip of peninsular Florida in the north. Crocodylus acutus occupies primarily coastal brackish water habitat, however it also occurs in freshwater to hypersaline habitats (Thorbjarnarson 2010. In Crocodiles. Status Survey and Conservation Action Plan. [Third Edition], American Crocodile Crocodylus acutus, pp. 46–53 S.C. Manolis and C. Stevenson. Crocodile Specialist Group, Darwin). There is limited literature on long distance movements of juvenile crocodilians worldwide and no literature on juvenile crocodiles in Florida. However, adult C. acutus in Florida have been documented to make seasonal movements of 5–15 km from preferred foraging habitat to nesting beaches (Mazzotti 1983. The Ecology of Crocodylus acutus in Florida. PhD Dissertation. The Pennsylvania State University, University Park, Pennsylvania. 161pp), and one adult was documented making a 35 km trip from her nest site to preferred foraging habitat (Cherkiss et. al. 2006. Herpetol. Rev. 38:72–73). Rodda (1984. Herpetologica 40:444–451) reported on juvenile C. acutus movement in Gatun Lake, Panama, and found that juveniles stayed within 1 km of their nest site for the first month. Movements of juvenile Crocodylus porosus (Saltwater Crocodile) in a river system in Northern Australia showed a maximum movement of 38.9 km from a known nest site, with the majority of the crocodiles staying within 15.6 km downstream to 6.8 km upstream (Webb and Messel 1978. Aust. Wildlife Res. 5:263–283). Juvenile movement of Crocodylus niloticus (Nile Crocodile) in Lake Ngezi, Zimbabwe showed crocodiles restricted their movements from 1.0 km up to 4.5 km through the wet and dry seasons (Hutton 1989. Am. Zool. 29:1033–1049). Long distance movements of alligators were recorded for sizes ranging from 28 cm to 361 cm in a coastal refuge in Louisiana, where the distance traveled ranged from 0.3 km to 90.2 km. The data showed that the smaller alligators moved greater distance than larger ones (Lance et al. 2011. Southeast Nat. 10:389–398). An ongoing 30 year mark and recapture study for Crocodylus acutus in Florida allowed us to look at long distance movement (>30 km) of juveniles (30km). Initial and most recent captures as a juvenile were used to analyze distances moved (Fig. 1). These distances were measured linearly between capture locations. Maximum linear distances of 76.3 km and 69.6 km were recorded for animals 4838 and 6662. All crocodiles moved from nesting habitat through potentially optimal nursery habitat prior to reaching their recapture locations. These juvenile long distance movements could be due to larger crocodiles facilitating their dispersal from the nest location (Lance et al. 2011. op. cit.). These data (Table 1.) support that there is exchange of individuals among the nesting colonies and our ongoing efforts to monitor this threatened species allow us to make observations of how juvenile crocodiles are moving throughout the landscape in an ecosystem currently undergoing restoration.

Herpetological Review