Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves

L. David Mech; Emily S. Almberg; Douglas Smith; Sagar Goyal; Randall S. Singer

doi:10.7589/0090-3558-48.2.473

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Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves

L. David Mech·Emily S. Almberg·Douglas Smith·Sagar Goyal·Randall S. Singer

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Abstract

Using real-time PCR, we tested 15 wolf ( Canis lupus ) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 10 4 50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.

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Journal of Wildlife Diseases Article

Source-reported bounding extent: 43.46089378008257° to 48.31242790407178° latitude; -111.07177734375° to -89.56054687499999° longitude. This indicates report coverage, not an exact sampling location. View area on OpenStreetMap.

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L. David Mech, Emily S. Almberg, Douglas Smith, Sagar Goyal, Randall S. Singer. 2012. Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves. https://doi.org/10.7589/0090-3558-48.2.473

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