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Advances and applications of Unoccupied Aerial Systems (UAS) research in landscape ecology

Landscape ecologists have long depended on satellite and aerial remote sensing to address questions about landscape pattern and process, structure, and change (Foody 2023 ). Unoccupied aerial systems/vehicles (UAS/UAV, a.k.a. drones) technology is becoming an increasingly popular research tool in environmental sciences allowing scientists to generate low-cost, high-quality, and high-resolution imagery on demand that can be tailored to specific research questions. While satellite data are of a fixed resolution and temporal interval, UAS offer researchers control and flexibility to design studies and collect data at resolutions and scales that provide ecologically relevant information at finer spatial resolutions (e.g., < 30 cm) than what is currently available from satellite platforms (typically > 3m), thus helping capture objects such as individual plant canopies, micro-topography, and individual animals. Unlike satellites with fixed orbits, UAS can be deployed at more optimal temporal frequencies for ecological monitoring. We organized the special collection “Advances and Applications of Unoccupied Aerial Systems (UAS) Research in Landscape Ecology” to showcase the many ways that UAS tools and technologies are currently applied to advance landscape ecological research. When we announced the collection in 2023, only 11 papers published in the journal Landscape Ecology used UAS data, which was a notably small number compared to many other general ecology, environmental science and remote sensing journals. In an attempt to understand why UAS were not more widely used in landscape ecology and provide possible solutions, we published a review article (Villarreal et al. 2025) that identified the challenges, knowledge gaps, and obstacles for the adoption of UAS technologies in landscape ecology research. The main issues we identified include: (1) an abundance of UAS methods papers in the existing literature, with comparatively few studies demonstrating how UAS can be applied to address ecological questions; (2) a perceived scale mismatch between the geographic extent of UAS data collection (local) compared to larger study areas (landscapes) and a need to design robust scaling approaches to connect fine-scale UAS data with broader ecological patterns; and (3) a need for improved integration of UAS data with other commonly used remote sensing datasets including historical high resolution aerial imagery. Additionally, researchers new to UAS remote sensing may be discouraged or overwhelmed by the general lack of scientific consensus and standardized protocols for typical tasks such as data collection, vegetation classification, and change detection, as well as restrictive and/or confusing policy, regulatory, and legal issues surrounding UAS operations (Villarreal et al. 2025).

Landscape Ecology

Rare earth element potential in coal and coal ash in the U.S. Gulf Coast

United States heavy reliance on imports of critical minerals (CMs), including rare earth elements (REEs), underscores the importance of development of domestic sources. The study objective was to quantify CM and REE concentrations in coal and coal ash in the US Gulf Coast region. CM and REE concentrations were measured for 118 samples from outcrops and 14 mines in the Gulf Coast. Results show that total REE + Yttrium (REY) concentrations (dry coal basis) are comparable to those of the upper continental crust (UCC) with localized hot spots, such as the Texas Gibbons Creek mine (REY ≤ ~ 2860 ppm). When normalized to UCC REY concentration (169 ppm, dry coal basis), REY to UCC ratios for Gulf Coast coal samples range from 0.1 to 17 (median ratio 0.6). REE extractability from lignites is high (median: 63%–93%) using environmentally benign weak acid. In addition to raw coal, coal ash from power plants could also serve as an REE source with a median ratio of REY in ash relative to coal of 4; however, extractability from coal ash is generally much lower (≤ 5% using the same weak acid as in coal). The median basket price for extracted REY as oxides from coal, assuming 70% extractability, is $3.2 per tonne of coal and $186 billion based on 58 billion metric tonnes of dry coal in the Gulf Coast. REEs important for magnets (Pr + Nd + Tb + Dy) account for ~ 80% of the total value. The corresponding median basket price for extracted REY as oxides from coal ash, assuming ~ 30% extractability, is ~$4.4 per tonne of ash and $1.2 billion based on 258 million tonnes of ash. REE production from coal would likely require co-products, such as activated carbon or humic acids, to attain economic viability. Production of REEs from coal ash could offset remediation costs related to potential water contamination. This reconnaissance study shows the potential for REE production from coal and coal ash in the Gulf Coast; however, carbon coproducts and/or societal benefits would likely be required for socioeconomic viability.

Alabama, Florida, Louisiana, Mississippi, Texas

Controlling invasive carp ichthyoplankton dispersion using a streamwise-oriented bubble screen: A proof-of-concept validation in a laboratory flume

Recent evidence of invasive grass carp ( Ctenopharyngodon idella ) reproducing in tributaries to the Laurentian Great Lakes has highlighted the need for control efforts targeting multiple life stages. Initial attempts to control dispersal of downstream-drifting invasive carp ichthyoplankton (i.e., eggs and larvae) using an oblique bubble screen (OBS) revealed that nearly neutrally buoyant grass carp eggs and larvae enter helical-like motions driven by the OBS, preventing aggregation within a single capture location. To improve dispersal control methods for such early-life stage carp, we used a laboratory flume to investigate the efficacy of a streamwise-oriented bubble screen to facilitate their near-bank capture. Five early-life stages of grass carp were tested: live water-hardened eggs, pre- and post-gas bladder inflation larvae, dead larvae, and dead eggs (preserved in formalin solution and later rehydrated). A range of mean channel velocities (0.23, 0.45, and 0.75 m/s) was tested for all drifters. Capture percentages increased with increasing airflow. Preserved eggs, for instance, showed capture percentages up to 95 %, 87 %, and 69 % at low, medium, and high water velocities for the highest airflow rate, respectively, in contrast with the lower than 5 % capture measured for zero airflow cases. Symmetric secondary flow structures on either side of the bubble screen induced helical trajectories of drifters and facilitated their capture in net-arrays along each wall. Velocity data were used to estimate helical recirculation timescales, enabling calculation of optimal bubble diffuser and net-array lengths for desired capture rates. This study provides useful guidance for the design of effective systems to control dispersal of downstream-drifting ichthyoplankton of invasive carp in streams.

Journal of Great Lakes Research

The Python Energy Balance model for Snow and Ice (PEBSI): Application and tradeoff analysis on Gulkana Glacier, Alaska

Glacier energy-balance models offer mechanistic insights into glacier mass balance under a changing climate, yet their considerable data requirements hinder large-scale applications. Here we present the open-source Python Energy Balance model for Snow and Ice (PEBSI), which includes physically based albedo evolution using the Snow, Ice and Aerosol Radiative (SNICAR) model. PEBSI is calibrated and validated using robust in situ data from Gulkana Glacier, Alaska from 2000 to 2024. Simulations forced with original and bias-corrected climate reanalysis data show that statistically downscaling reanalysis data with in situ observations is necessary to reproduce summer mass balance (mean absolute error [MAE] = 0.75 m w.e. vs 0.22 m w.e., respectively). A grid search across two parameters, a precipitation factor and a densification parameter, reveals tradeoffs in performance compared to seasonal mass balance and end-of-winter snow density and depth. No single combination of parameters minimizes all errors, underscoring the inherent overparameterization of energy-balance models and challenges with translating coarse climate data to the glacier scale. The calibrated model successfully simulates the 2024 melt season, agreeing with surface-height change (MAE = 0.48 m) and albedo (MAE = 0.066) observations. Moving forward, PEBSI provides unique opportunities to quantify albedo feedbacks and their impact on present and future glacier mass loss.

Alaska

Tag retention, growth, condition, and survival of externally marked Yellow Perch

Objective: Determining the movement and survival of Yellow Perch Perca flavescens that inhabit Lake Erie is a priority management concern, yet contemporary understanding of their cross-jurisdictional movements and exploitation is lacking. To support future movement and survival studies, we evaluated the retention rates, condition, growth, and survival associated with four external tagging methods. Methods: One-hundred hatchery- reared Yellow Perch (188–257 mm) were tagged and held in recirculating aquaculture systems over a 168- d period. We tested four external tag types, including loop tags, ring tags, streamer tags, and T- bar anchor tags, and quantified tag retention rates, as well as tagging effects on condition, growth, and survival. Results: T- bar anchor and loop tags had 100% retention, whereas ring (54%) and streamer (0%) retention was poor. The condition and growth of fish were not affected by any of the tagging methods; however, the loop tags caused abrasive wounds near the tagging site that could lead to secondary infection and latent effects on survival. We found no evidence that tagging resulted in mortality. Conclusion: External T- bar tags were the most efficient to apply and provided a marking option that was highly visible, had high retention, and had no effects on condition, growth, or survival in Yellow Perch over the 168- d study period.

North American Journal of Fisheries Management

Relationship of satellite-derived fire severity to archaeological fire severity and fire effects in the Jemez Mountains, New Mexico, USA

Background Archaeological attributes important for determining cultural affiliation, site age, or artifact origin can be damaged or destroyed by wildfires. Satellite-derived fire severity products are used in post-fire rapid response to prioritize and guide stabilization and preservation activities, but their ability to predict archaeological fire impacts has not yet been tested. We compared the severity of fire from Burned Area Reflectance Classification (BARC) maps with ground-based observations of fire severity (“archaeological fire severity”) and archaeological impacts for 904 prehistoric or historic sites within three wildfires in the Jemez Mountains of north-central New Mexico, U.S. Results from this unique study inform archaeological preservation in the context of wildfires and fire management. Results We found a statistically significant, moderately strong association between BARC and archaeological fire severity and a high likelihood that sites with a low BARC fire severity also had a low archaeological fire severity designation. Sites of moderate or high BARC severity were less likely to have a matching severity classification in post-fire assessments, and BARC maps tended to identify as unburned areas where archaeologists determined that sites were burned at low severity. Wildfire of any severity resulted in measurable impacts to archaeological architecture, features, or artifacts at some sites, with increasing proportion of sites impacted at moderate versus low BARC severity, but fire impacts were also observed at sites classified by BARC as unburned. Conclusions Generally, BARC maps provide good assessment of the severity of fire and its effects on archaeological resources, particularly for areas burned at low severity. In areas of moderate or high fire severity, BARC maps may not consistently identify sites with a high need for assessment, and archaeological fire effects can also be present at sites classified by BARC as unburned. Adoption of standardized post-fire archaeological assessments across wildfires and regions, exploration of alternative fire mapping products, and continued research on the fire environments that produce archaeological fire effects can enhance archaeological preservation and post-fire response.

New Mexico

Distribution and abundance of Least Bell’s Vireos ( Vireo bellii pusillus ) and Southwestern Willow Flycatchers ( Empidonax traillii extimus ) at the Mojave River Dam, San Bernardino County, California—2025 Data Summary

Executive Summary We surveyed for Least Bell’s Vireos ( Vireo bellii pusillus ; vireo) and Southwestern Willow Flycatchers ( Empidonax traillii extimus ; flycatcher) at the Mojave River Dam study area near Hesperia, California, in 2025. Four vireo surveys were completed between April 23 and June 26, 2025, and three flycatcher surveys were completed between May 16 and June 26, 2025. We detected two territorial male vireos, both of which were paired, and one transient vireo. No juvenile vireos were observed during surveys. Vireos were reported in two habitat types: riparian scrub dominated by narrowleaf willow ( Salix exigua ) or mule fat ( Baccharis salicifolia ) and willow-cottonwood dominated by red or arroyo willow ( Salix laevigata or lasiolepis ). One transient willow flycatcher of unknown subspecies was observed in willow-cottonwood habitat dominated by Fremont cottonwood ( Populus fremontii ).

California

Ungulate migrations of the Western United States, volume 6

This report, volume 6 in the “Ungulate Migrations of the Western United States” report series, showcases the migrations of 23 ungulate herds in the Western United States. The report series is produced by the Corridor Mapping Team (CMT). Led by the U.S. Geological Survey, the CMT is a collaboration among 11 State agencies, as well as regional and Federal partners, and an expanding number of Tribal wildlife agencies. The CMT was initiated in response to the U.S. Department of the Interior Secretarial Order 3362, which was signed in 2018 and provided Federal support to expand existing research efforts to study ungulate populations and conserve their migrations throughout the Western United States. Including this volume, the report series has detailed the migrations of 237 unique ungulate herds throughout the Western United States and continues to serve as a valuable resource to guide local and regional management, policy, and on-the-ground work necessary to maintain intact and functional ungulate migrations. This report highlights several guiding principles of the CMT that facilitate collaboration among the diverse set of partners and contribute to the program’s continued successes. Notably, raw global positioning system data are not shared among participating agencies and the U.S. Geological Survey, delineating migration corridors and seasonal ranges relies on empirical data, the CMT provides flexible approaches to participating State and Tribal partners, and regular CMT meetings create a framework for open communication among agency partners that supports transboundary mapping of migrations. The 237 ungulate migrations that have been included in the report series are an expanding inventory, which can help maintain ungulate migrations in perpetuity.

Arizona, California, Colorado, Idaho, Montana, Nev

Thermal and dielectric properties of Juno’s regolith at one millimeter wavelength

We present the modeling results of the thermal lightcurve of asteroid (3) Juno at the wavelength of λ = 1.3 mm measured by the Atacama Large Millimeter-submillimeter Array. A thermophysical model together with a radiative transfer model suggests a thermal inertia of 13 ± 10 [J m −2 K −1 s −0.5 ], an equivalent emissivity of 0.8 ± 0.1, a loss tangent of 0.4 ± 0.3, and an index of refraction 1.8 ± 0.3. Based on previous laboratory measurements, the modeled index of refraction suggests a regolith porosity of about 45%. However, thermal inertia models using the material parameters of ordinary chondrite indicate a grain size of ∼10 μ m and require a high porosity of ∼90% to explain the low thermal inertia. In order to explain such a contradiction, we postulate that some repulsive mechanism might be in effect to reduce the contact of grains and therefore the thermal inertia. The loss tangent of Juno’s regolith corrected for the modeled thermal skin depth is in the order of 0.5, much higher than that of the lunar regolith and indicating an electrical skin depth of L = 0.1–1.4 mm that is within the thermal skin depth. The shape of the rotational lightcurve of Juno in the millimeter wavelengths is dominated by its irregular shape, but rotational variations in the thermal and/or dielectric properties cannot be ruled out. Our results demonstrate that millimeter-wavelength observations of asteroids provide an extra dimension of constraints to the porosity and grain size of asteroid regolith compared to the thermal infrared observations.

Planetary Science Journal

Status of round goby invasion fronts in New York and Quebec: Implications for Lake Champlain

Invasive round goby Neogobius melanostomus have advanced eastward through the state of New York and provinces of Ontario and Quebec over the past two decades and are approaching Lake Champlain, one of the largest lakes in North America. This manuscript describes international efforts to monitor round goby populations during 2021–2025 on (a) the southern approach to Lake Champlain via the Hudson River and Champlain Canal, and (b) the northern approach to Lake Champlain via the Saint Lawrence River and Richelieu River. Monitoring utilized environmental DNA (eDNA), backpack electrofishing, beach seining, benthic trawling, and viral hemorrhagic septicemia virus (VHSV) testing. In the Champlain Canal, round goby were captured as far north as the downstream side of the C1 dam (97 kilometers [km] from Lake Champlain) while eDNA detections occurred as far north as the upstream side of the C2 dam (90 km from Lake Champlain). In the Richelieu River, round goby were captured as far south as Saint-Marc-sur-Richelieu (82 km from Lake Champlain) while the southern-most eDNA detections occurred near the Canadian side of the international border (4 km from Lake Champlain). Water temperature influenced habitat usage of round goby in the Champlain Canal, with catch rates in near-shore areas declining at < 10 °C. All VHSV test results were non-detections at the mouth of the Richelieu River, while one positive and two inconclusive results occurred along the Champlain Canal. Together, these data have informed multiple mitigation measures and have implications for management of aquatic invasive species across North America.

BioRxiv

Leveraging local species data, a global database, and an occupancy model to explore bee–plant interactions

Global declines in bee populations are threatening the ecosystem services they provide, including pollination. Many bee–plant interactions are understudied, producing an incomplete understanding of resulting ecosystem-level vulnerabilities. The last decade has generated a wealth of opportunistic data originating from natural history collection records, published ecological datasets, and citizen/community science initiatives in online databases such as Global Biotic Interactions (GloBI). Here, we explore hypotheses related to bee–plant interactions and detection processes using the GloBI database, curated checklists of bee and flowering plant species, and an occupancy model. We hypothesized that larger, social bees would visit a larger number of plant species, while smaller, solitary bees would visit fewer. We also predicted that flowers with open, bowl-like shapes would attract a greater diversity of bee visitors compared to closed shapes. Further, we hypothesized that both floral and bee traits, such as bright colors and conspicuous patterns, would increase detectability, and that different data collection methods would vary in their ability to capture bee–plant interactions. Lastly, we hypothesized that the interaction network generated by the output of the occupancy model, which accounted for imperfect bee–plant detection, would yield more interactions, thereby increasing measures of evenness and decreasing nestedness and specialization, as compared to the network generated from recorded interaction data. We found that smaller bees exhibited higher probabilities of plant interactions than larger bees, but we did not find evidence that bee sociality influenced the probability of interacting with plants. We found that blue flowers and closed (not-bowl-shaped) flowers had higher probabilities of bee-plant interaction than other flower colors or bowl-shaped flowers, respectively. We also found that larger bee size, blue flowers, bowl shapes, and community science sources were associated with higher detection probabilities of bee–plant interactions. Lastly, the interaction network generated by the occupancy model output showed higher levels of evenness, nestedness, and connectance than the network generated by the GloBI data. Our study is among the first to utilize occupancy modeling to directly model species' interactions, leverage aggregated, open-source databases and expert checklists, and highlight the influence of detection and collection biases on our understanding of ecological interactions.

California

Cost of migration increased during an outbreak of highly pathogenic avian influenza virus

Migration is thought to be costly such that challenges faced during migration likely affect how birds migrate through direct selection on migratory behavior. Survival throughout the annual cycle and conditions that affect the survival costs of migration are therefore critical to our understanding of the causes and consequences of migration. Estimating daily survival throughout the annual cycle of migratory birds has only recently become possible. We used over a decade of GPS tracking data from 186 migratory Buteo lagopus to quantify causes of mortality as well as demographic, seasonal, and spatial variation in daily survival and how survival consequences of migration changed during an outbreak of highly pathogenic avian influenza virus. The cost of migration via direct mortality varied between avian influenza and baseline study periods. Surprisingly, mortality risk during typical spring migrations was lower than in all other seasons, but mortality risk during the avian influenza outbreak was higher during both spring and fall migrations. Additionally, we found increased mortality risk in females during the avian influenza outbreak but no sex differences during baseline periods. Hence, the relative cost of migration was altered by the virus. During baseline periods, mortality risk was highest during late fall migration and winter, coinciding with increased anthropogenic-related mortalities at lower latitudes. However, the number of deaths caused by avian influenza (n = 11) in a single year equaled the total number of deaths caused by anthropogenic-related factors (n = 11; primarily collision trauma and persecution) across all 12 study years. Overall, our study highlights how added stressors such as disease outbreaks and anthropogenic factors may exacerbate existing seasonal constraints by directly increasing mortality risk.

Journal of Avian Biology

Compact seismicity bursts have different characteristics from regional seismicity

Earthquakes tend to cluster, developing into sequences driven by stress perturbations and transient fault-zone processes. Depending on the driving process, earthquake sequences show differing behaviors. This variability challenges our ability to observe or distinguish these driving processes in high resolution. Here we systematically identify seismicity bursts throughout southern California using new statistical methods and examine their causes with other independent geophysical observations. Seismicity bursts are defined as statistically significant seismicity rate anomalies. Our approach has the advantage of being data driven, depending on few earthquake occurrence assumptions. We find abundant seismicity bursts across southern California, most frequently occurring along the San Jacinto Fault and in the Salton Sea and Coso geothermal fields. These seismicity bursts are highly compact in space and time, often encompassed by a 5 km radius and have durations less than 10 hr. Many of the seismicity bursts have their largest earthquake near the beginning of the sequence, but the largest earthquake is usually not an obvious mainshock. We utilize a variety of independent geophysical data sets to analyze the characteristics of the seismicity bursts, finding that the seismicity bursts have low b-values, low spectral stress drops, and varied stress ratios compared to regional seismicity. These differences suggest that seismicity bursts are driven by transient processes acting frequently across fault networks.

California

Tidal wetland soil carbon accumulation rates for coastal California

Carbon stock and carbon accumulation rate data are vital to multiple aspects of tidal wetland conservation and restoration policy. In California, USA tidal soil data are rare outside of the San Francisco Bay and Sacramento Delta regions, despite the differing conditions experienced by the outer coastline. Here we provide carbon stocks and decadal-to-centennial-scale carbon accumulation rate calculations. This dataset presents 83 soil depth profiles from 15 sites, with 58 cores from 12 tidal wetland sites analyzed for carbon stock, mostly from the outer coastline of California. Mean organic matter content was 11%, and stocks estimated to 1 meter depth ranged from 15.4 to 44.7 kgC m −2 . Organic matter content generally declined asymptotically with depth. Carbon accumulation rates ranged from 39.2 to 130.0 gC m −2 yr −1 . Neither carbon stock nor carbon accumulation rates were notably different from global average values. Data at this level of reporting are vital for establishing restoration baselines, informing greenhouse gas mitigation planning, and projecting future ecosystem response to sea-level rise.

California

Changes in spatial distribution and abundance together determine potential for population persistence for greater sage-grouse

Aim Population ecologists often focus on changes in the distribution and abundance of wildlife species, which are useful for trend analyses and status assessments. However, rarely are these responses evaluated simultaneously for a single species, despite their unique contributions to fully assess a species' viability. For example, focusing solely on total abundance can mask important losses in overall distribution within a metapopulation structure that may contribute to long-term population instability that results from the extirpation of small peripheral populations. Location Bi-State region of Nevada and California, USA. Methods We simultaneously evaluated changes in population abundance and distribution for greater sage-grouse (hereafter sage-grouse; Centrocercus urophasianus ) within the Bi-State Distinct Population Segment (DPS), a genetically distinct and isolated population straddling the border of Nevada and California. We combined population counts, demographic data, and information on space use from marked individuals to evaluate changes in population distribution and abundance over three time periods that corresponded to the three most recent population nadirs (1995–2019, 2002–2019 and 2008–2019). Results The Bi-State DPS exhibited evidence of ~1.2%–2.5% declines annually, over the short/medium-term (1995–2019; λ^ = 0.987, 95% CRI: 0.970–0.999), short-term (2002–2019; λ^ = 0.975, 95% CRI: 0.963–0.985) and recent-term (2008–2019; λ^ = 0.988, 95% CRI: 0.973–1.001). Since 1995, the spatial distribution of sage-grouse abundance in the Bi-State DPS shifted amongst subpopulations, with peripheral subpopulations suffering the largest declines. Main Conclusions Gains in abundance and distribution amongst expanding subpopulations did not offset losses in the remaining subpopulations, with a net loss in occupied distribution of 156 km 2 since 1995. Reductions in spatial distribution could have implications for metapopulation persistence as peripheral populations become more vulnerable to stochastic events, which would not have been apparent from the evaluation of overall metapopulation abundance on its own.

California, Nevada

Local and landscape-level environmental conditions drive habitat selection across terrestrial mammal species

Aim Animal movements are a fundamental process affecting communities and ecosystems. Quantifying habitat selection across species and habitats is key for understanding how animals respond to environmental change. Currently, we lack comparative studies that examine how habitat selection varies across species traits and landscapes. We aim to quantify global patterns of habitat selection to help understand the fundamental drivers of movement behaviour. Location Global. Time Period Contemporary. Major Taxa Studied Terrestrial mammals. Methods We estimated selection coefficients for terrain ruggedness, vegetation productivity, human population density and distance to roads of individual terrestrial mammals through step-selection analysis of 1344 GPS tracks across 48 species. We quantified intra- and interspecific variation and tested whether selection coefficients were associated with species traits and habitat availability. Results We observe an overall avoidance of roads and areas of high human population density as well as rugged terrain, with a large proportion of individuals selecting for areas of intermediate NDVI. However, we also found large intraspecific variation in habitat selection and show that this variation is predicted by local and landscape-level environmental conditions rather than species traits. Individuals in more remote areas exhibited weaker functional responses to human presence than those in more disturbed areas. Avoidance of rugged terrain is also context-dependent, with stronger avoidance when local ruggedness is high. The only exception to the observed intraspecific variability is consistent species-level responses to road proximity. Main Conclusions Our findings contribute to the understanding of habitat selection by terrestrial mammals, showing that selection is largely shaped by environmental conditions and that animals exhibit high plasticity in their responses. Our results also provide further evidence for the significant impact of roads on animal movement. These insights can help us to understand the potential effects of environmental change on the behaviour of mammal species around the world.

Global Ecology and Biogeography

Population trends of dabbling ducks wintering in the alluvial valleys of Arkansas and Mississippi

Population abundances, distributions, and compositions across a diversity of taxa are changing, partly as a consequence of human-induced global modifications. Although linking population fluctuations to anthropogenic-induced alterations can be challenging, it is increasingly clear that long-term monitoring is critical to understanding changing populations. For waterfowl, concerns over recent declines in breeding populations or possible redistribution from using traditional wintering grounds for some species support continued monitoring of regional populations. To further understand population trends on traditional wintering grounds, we examined abundance trends over time within the Mississippi and Arkansas portions of the Mississippi Alluvial Valley, using two sources of data: systematic aerial surveys and citizen science data. We found great annual variability in rates of population change ( r ranging from −1.04 to 0.71) in five species of dabbling ducks. Despite this annual variation, green-winged teal Anas carolinensis , mallards Anas platyrhynchos , northern pintail Anas acuta , and northern shoveler Spatula clypeata showed no significant trends in population size over approximately 15 years in Mississippi and Arkansas, but gadwall Mareca strepera showed a 4% annual increase in the citizen science data for Mississippi. Despite substantial variation in annual abundance, the timing of peak abundance was remarkably consistent from year to year, with peaks typically occurring in January. Our results demonstrate that autumn–winter population numbers of dabbling ducks can be quite variable interannually, but they lack a consistent trend in the Mississippi Alluvial Valley during the past two decades. Going forward, investigation into how citizen science data could be leveraged to increase the precision of trends within this landscape might be of benefit, and sampling efforts could perhaps be added towards the latter portion of the wintering period. As populations continue to adjust in a changing world, maintaining monitoring surveys in regions important to waterfowl is an effective and important means of evaluating trends.

Arkansas, Illinois, Kentucky, Louisiana, Mississip

Genomic selection for low salinity tolerance in the eastern oyster Crassostrea virginica in Louisiana and Chesapeake Bay populations

The use of genomic selection (GS) to improve traits of interest in aquaculture species is growing with the availability of genomic resources for those species. The eastern oyster , Crassostrea virginica , is a major aquaculture product and keystone species along the U.S. mid-Atlantic and Gulf coasts. Populations and production of C. virginica are threatened by several environmental stressors, and in certain coastal regions, episodic low salinity conditions have contributed to mass mortality events. Here we examined the potential for genomic selection to improve tolerance to extreme low salinity (≤ 2) in eastern oysters and report the evaluation of GS models for C. virginica from two regions- Louisiana and Chesapeake Bay. Tissue samples from both mortalities and survivors of the lab-based salinity challenges (salinity 2, 28 °C) were genotyped on the 66 K C. virginica SNP array. We performed a genome wide association study (GWAS) for both challenges and then evaluated the effects of several different parameters on GS prediction accuracy, including model type (GBLUP, Bayesian, and weighted), data filtering, Bayesian modelling parameters, and trait encoding (Gaussian, ordinal, and censored). GWAS revealed a significant region on chromosome 1 in the Chesapeake animals while the Louisiana animals showed only a few significantly associated loci. GS model type had no significant impact on prediction accuracy in the Chesapeake animals, but a large impact in the Louisiana animals. Likewise, prediction accuracy remained consistent in the Chesapeake Bay challenge with as few as 5000 markers, while accuracy in the Louisiana challenge began to decline with fewer than 25,000 markers. This likely reflects the lower relatedness among the Louisiana individuals due to unintended wild overset. Overall, with moderate prediction accuracies (∼0.45–0.55) in both regions, GS shows the potential to improve low salinity tolerance in eastern oysters. This will be beneficial to selective breeding programs and restoration efforts in regions where this trait improves oyster survival.

Louisiana, Maryland, Virginia