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Results for “Journal of Applied Phycology”

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4 recordsLinked to original sources
USGS2005

A direct immunoassay for detecting diatoms in groundwater as an indicator of the direct influence of surface water

Groundwater under the direct influence of surface water (GWUDISW) is of concern in communities where growing public demand on groundwater resources has resulted in increased withdrawals and hydraulic stress near surface water bodies. Under these conditions, contaminants such as methyl-tert butyl ether (MTBE) and biological materials have been detected in domestic wells. Other contaminants and pathogens associated with surface water are not routinely tested for in groundwater-supplied systems. To address the need for methods to easily identify potentially vulnerable supplies, a direct immunoassay for the quantitative detection of diatoms in raw water samples was developed as a measure of surface water influence on groundwater. Cell wall preparations from Nitzschia palea Kützing, a freshwater diatom found throughout North America, were used to produce a polyclonal antibody that was applied in a direct enzyme-linked immunosorbent assay (ELISA) developed to detect the presence of N. palea cell wall components. The direct immunoassay allows detection at 500 cells L −1 , a level similar to diatom concentrations observed in samples of groundwater collected near the test site. This investigation was the first attempt to utilize an ELISA as an indicator of surface water influence on groundwater. Further research is needed to develop more specific diatom-based monoclonal antibodies, determine cross-reactivity, and optimize sample processing and ELISA procedures for development of a standardized method.

Journal of Applied Phycology↗
USGS2021

Growth inhibition of the harmful alga Prymnesium parvum by plant-derived products and identification of ellipticine as highly potent allelochemical

Prymnesium parvum is a toxin-producing harmful alga that has caused ecological and economic damage worldwide. Effective methods to control blooms of this species in the field, however, are unavailable. This study examined five natural compounds present in the invasive plant Arundo donax and one synthetic derivative (5,6-dichlorogramine) for their effect on P. parvum growth. All compounds except one inhibited growth in the following order of potency: ellipticine > > 5,6-dichlorogramine > 1 H-indole = 2,4,6-trimethyl-benzonitrile > gramine. Ellipticine was by far the most potent inhibitor, with full algicidal activity at concentrations as low as 0.04 mg L −1 and 3- and 9-day IC 50 values of 0.012 and 0.007 mg L −1 , respectively. A reduction in chlorophyll content and swimming activity and an increase in length and volume (swelling) were documented in algal cells exposed to 0.01–0.02 mg ellipticine L −1 . These results show that ellipticine is among the most potent natural algicides identified to date. The sixth compound tested, oleamide, unexpectedly stimulated algal growth above control levels. Overall, these observations confirm the existence of highly potent anti- P. parvum allelochemicals in giant reed and demonstrate potential for using products derived from this plant in the development of natural, environmentally friendly methods to control harmful algal blooms.

Journal of Applied Phycology↗
USGS2003

Biochemical composition of three algal species proposed as food for captive freshwater mussels

To identify potential diets for rearing captive freshwater mussels, the protein, carbohydrate (CHO), and lipid contents of two green algae, Neochloris oleoabundans, Bracteacoccus grandis, and one diatom, Phaeodactylum tricornutum, were compared at different growth stages. The fatty acid and sterol composition were also identified. Protein was greatest (55-70%) for all species at late log growth stage (LL), and declined in late stationary (LS) growth. CHO was greatest at LS stage for all species (33.9-56.4% dry wt). No significant change in lipid levels occurred with growth stage, but tended to increase in N. oleoabundans. Mean lipid content differed significantly in the order: N. oleoabundans > P. tricornutum > B. grandis. Total fatty acids (TFA) were higher at LS stage compared to other stages in the two green algae, and stationary stage in the diatom. Mean unsaturated fatty acids (UFA) as %TFA was significantly higher in N. oleoabundans than the other species. The green algae contained high percentages of C-18 polyunsaturated fatty acids (PUFAs), while the diatom was abundant in C-16 saturated and mono-unsaturated fatty acids and C-20 PUFA fatty acids. Growth stage had no effect on sterol concentration of any species. B. grandis showed significantly higher sterol levels than the other species except P. tricornutum at S stage. B. grandis was characterized by predominantly ??5, C-29 sterols, while N. oleoabundans synthesized ??5,7, ??5,7,22, and ??7, C-28 sterols. P. tricornutum produced primarily a ??5,22, C-28 sterol, and a small amount of a ??7,22, C-28 sterol.

Journal of Applied Phycology↗
USGS2019

Temperature mediates secondary dormancy in resting cysts of Pyrodinium bahamense (Dinophyceae)

High‐biomass blooms of the toxic dinoflagellate Pyrodinium bahamense occur most summers in Tampa Bay, Florida, USA, posing a recurring threat to ecosystem health. Like many dinoflagellates, P. bahamense forms immobile resting cysts that can be deposited on the seafloor—creating a seed bank that can retain the organism within the ecosystem and initiate future blooms when cysts germinate. In this study, we examined changes in the dormancy status of cysts collected from Tampa Bay and applied lessons from plant ecology to explore dormancy controls. Pyrodinium bahamense cysts incubated immediately after field collection displayed a seasonal pattern in dormancy and germination that matched the pattern of cell abundance in the water column. Newly deposited (surface) cysts and older (buried) cysts exhibited similar germination patterns, suggesting that a common mechanism regulates dormancy expression in new and mature cysts. Extended cool‐ and warm‐temperature conditioning of field‐collected cysts altered the cycle of dormancy compared with that of cysts in nature, with the duration of cool temperature exposure being the best predictor of when cysts emerged from dormancy. Extended warm conditioning, on the other hand, elicited a return to dormancy, or secondary dormancy, in nondormant cysts. These results directly demonstrate environmental induction of secondary dormancy in dinoflagellates—a mechanism common and thoroughly documented in higher plants with seasonal growth cycles. Our findings support the hypothesis that a seasonal cycle in cyst germination drives P. bahamense bloom periodicity in Tampa Bay and point to environmentally induced secondary dormancy as an important regulatory factor of that cycle.

Florida↗