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Partitioning of chemicals in aquatic organisms

Human populations have utilized heavy metals and persistent organic pollutants for their physiochemical properties in industrial, agricultural, and consumer goods for decades. Limited knowledge on their persistence and toxicological effects has resulted in organisms being exposed to some of the most problematic compounds ever generated by humans. Although overlap in exposure paradigms exists for historical and emerging contaminants, the different physiochemical properties, sources into the environment, and bioactivity of contaminants of emerging concern (CECs) have highlighted the importance of characterizing their risk to aquatic wildlife under chronic low-dose exposure scenarios. This chapter defines the fundamental terminology associated with characterizing the exposure paradigm in ecological risk assessment. The different sources and fate, routes of exposure, and biotransformation of common contaminants are covered using model chemicals to emphasize important factors that affect their partitioning among different environmental matrices. Finally, this chapter concludes with a discussion about bioaccumulation models and an example of how two similar CECs demonstrate different clearance rates and bioaccumulation potentials in fish.

Book chapter

Using an ecological ethics framework to make decisions about the relocation of wildlife

Relocation is an increasingly prominent conservation tool for a variety of wildlife, but the technique also is controversial, even among conservation practitioners. An organized framework for addressing the moral dilemmas often accompanying conservation actions such as relocation has been lacking. Ecological ethics may provide such a framework and appears to be an important step forward in aiding ecological researchers and biodiversity managers to make difficult moral choices. A specific application of this framework can make the reasoning process more transparent and give more emphasis to the strong sentiments about non-human organisms held by many potential users. Providing an example of the application of the framework may also increase the appeal of the reasoning process to ecological researchers and biodiversity managers. Relocation as a conservation action can be accompanied by a variety of moral dilemmas that reflect the interconnection of values, ethical positions, and conservation decisions. A model that is designed to address moral dilemmas arising from relocation of humans provides/demonstrates/illustrates a possible way to apply the ecological ethics framework and to involve practicing conservationists in the overall decision-making process. ?? 2008 Springer Science+Business Media B.V.

Science and Engineering Ethics

The use of discontinuities and functional groups to assess relative resilience in complex systems

It is evident when the resilience of a system has been exceeded and the system qualitatively changed. However, it is not clear how to measure resilience in a system prior to the demonstration that the capacity for resilient response has been exceeded. We argue that self-organizing human and natural systems are structured by a relatively small set of processes operating across scales in time and space. These structuring processes should generate a discontinuous distribution of structures and frequencies, where discontinuities mark the transition from one scale to another. Resilience is not driven by the identity of elements of a system, but rather by the functions those elements provide, and their distribution within and across scales. A self-organizing system that is resilient should maintain patterns of function within and across scales despite the turnover of specific elements (for example, species, cities). However, the loss of functions, or a decrease in functional representation at certain scales will decrease system resilience. It follows that some distributions of function should be more resilient than others. We propose that the determination of discontinuities, and the quantification of function both within and across scales, produce relative measures of resilience in ecological and other systems. We describe a set of methods to assess the relative resilience of a system based upon the determination of discontinuities and the quantification of the distribution of functions in relation to those discontinuities. ?? 2005 Springer Science+Business Media, Inc.

Ecosystems

Integration of social and ecological sciences for natural resource decision making: Challenges and opportunities

The last 25 years have witnessed growing recognition that natural resource management decisions depend as much on understanding humans and their social interactions as on understanding the interactions between non-human organisms and their environment. Decision science provides a framework for integrating ecological and social factors into a decision, but challenges to integration remain. The decision-analytic framework elicits values and preferences to help articulate objectives, and then evaluates the outcomes of alternative management actions to achieve these objectives. Integrating social science into these steps can be hindered by failing to include social scientists as more than stakeholder-process facilitators, assuming that specific decision-analytic skills are commonplace for social scientists, misperceptions of social data as inherently qualitative, timescale mismatches for iterating through decision analysis and collecting relevant social data, difficulties in predicting human behavior, and failures of institutions to recognize the importance of this integration. We engage these challenges, and suggest solutions to them, helping move forward the integration of social and biological/ecological knowledge and considerations in decision-making.

Environmental Management

Anticoagulant rodenticides and wildlife: Concluding remarks

Rodents are known to affect human society globally in various adverse ways, resulting in a widespread demand for their continuous control. Anticoagulant rodenticides (ARs) have been, and currently remain, the cornerstone of rodent control throughout the world. Although alternative control methods exist, they are generally less effective. ARs work by affecting vitamin K metabolism, thereby preventing the activation of blood clotting factors and eventual coagulopathy. Since ARs are non-selective, their undoubted benefits for rodent control have to be balanced against the environmental risks that these compounds pose. Although they have been used for decades, pharmacokinetic and toxicokinetic data are mainly available for laboratory mammals and have concentrated on acute effects. Limited information is available on chronic exposure scenarios and for wildlife species. Important gaps exist in our understanding of the large inter- and intra-species differences in sensitivity to ARs, especially for non-target species, and in our knowledge about the occurrence and importance of sub-lethal effects in wildlife. It is clear that mere presence of AR residues in the body tissues may not indicate the occurrence of effects, although unequivocal assessment of effects under field conditions is difficult. Ante-mortem symptoms, like lethargy, subdued behaviour and unresponsiveness are generally not very specific as is true for more generic post-mortem observations (e.g. pallor of the mucous membranes or occurrence of haemorrhages). It is only by combining ante or post-mortem data with information on exposure that effects in the field may be confirmed. We do know however that a wide variety of non-target species are directly exposed to ARs. Secondary exposure in predators is also widespread although there is limited information on whether this exposure causes actual effects. Exposure is driven by ecological factors and is context specific with respect to spatial habitat configuration and bait placement. Another key factor that affects the interaction between ARs and wildlife is the development of resistance in target species. The development of resistance has resulted in higher use of SGARs, thereby increasing the potential of non-target and secondary exposure. AR use has increasingly become more strictly regulated, increasing the need for alternatives. Alternatives are available, including non-anticoagulant rodenticides, but these may also pose significant risk to environmental organisms, humans and pets. There are also various mitigation measures that can be implemented when using ARs, including bait protection, pulsed baiting at the onset of infestation, restricting use by non-professionals, and avoiding use in areas of high non-target density. Reduction in secondary exposure may result from e.g. non-chemical control, habitat management, and, in agricultural habitats, the use of lure crops and supplemental feeding. Such Integrated Pest Management (IPM) may not only reduce non-target exposure but also benefit resistance management. Barriers to adopt IPM approaches however, include the perception that they do not work or too slowly and are more laborious, expensive and time consuming. It is therefore important that the expectations of stakeholders are considered and managed. Nevertheless, further development of alternatives and IPM measures is essential, so the key research priority related to rodent control may ultimately be to address the lack of scientific assessment of the effectiveness of both specific AR mitigation measures and of IPM approaches to rodent control.

Book chapter

Effects of metal-mine drainage on water quality in selected areas of Colorado, 1972-73

Colorado is a child of mining. In the past, towns such as Cripple Creek, Leadville, Creede, and many others were the lifeblood of the economy. While metal mining is still economically important to Colorado, another legacy of this past activity is attracting increasing attention—the detrimental effects of drainage from abandoned and active mines and tailings piles (waste rock piles) on streams. Concern with metal-mine drainage is due to the acid resulting from oxidation of pyritic material. The acid itself can be harmful to aquatic organisms, and can make the water unusable for various recreational and industrial needs. Just as importantly, however, the acid dissolves metals from ores and tailings piles and releases them into the streams. Many of the metals are toxic to aquatic organisms, humans, and livestock; and, like excess acidity, the metals can make streams unusable for some types of recreation and industry. In July 1971, a study of the effects of both metal- and coal-mine drainage on surface-water quality in Colorado was undertaken by the U.S. Geological Survey in cooperation with the Colorado Water Pollution Control Commission. Based on the reconnaissance phase of this project (Wentz, 1974), 17 areas judged to be adversely affected by metal-mine drainage were chosen for further study. One additional area was included because it is the only place found in Colorado that potentially could be affected by coal-mine drainage. The present report summarizes the intensive study of these areas; it is the second product of the mine-drainage study. The first report was published as Colorado Water Resources Circular 21.

Colorado

Organic matter integration, overprinting, and the relative fraction of optically active organic carbon in a human-impacted watershed

Rivers continually integrate terrestrial organic matter (OM) into their waters, in a process that transfers 1.9 Pg C yr –1 as the primary linkage between oceanic and terrestrial carbon cycles. Yet rivers are not simple, conservative OM integrators. Patchy local land uses (wetlands, bogs, agriculture) release OM that can disproportionately alter river biogeochemistry and overprint upstream carbon. These releases are quantifiable at the plot scale but remain unpredictable across river reaches and watersheds, critically inhibiting our ability to scale up terrestrial-aquatic linkages to regional/global carbon cycling models. We evaluated OM overprinting distance along a human-influenced watershed to quantify river integration of terrestrial OM and to bridge the quantification gap between habitats and waterway biogeochemistry. We investigated changes in dissolved organic carbon (DOC) concentration and dissolved organic matter (DOM) composition (lignin phenols, fluorescence excitation-emission spectra using parallel factor analysis [PARAFAC], and the relative fraction of optically active DOM [EEM DOC ]). DOC concentrations increased continually ( p < 0.001) downstream, from median 1.0 mg L –1 at 30 km (headwaters) to 3.3 mg L –1 at the river mouth. This rate of increase corresponded to a DOC overprinting distance—the longitudinal distance over which DOC concentrations double—of 13 km. Mainstem DOC overprinting distance ranged from 8 km (winter, rainy season) to 21 km (summer, dry season with irrigation), highlighting stronger overprinting during increased hydraulic connectivity. Stronger overprinting also correlated to higher EEM DOC ( p < 0.001). Overprinting distance effectively quantifies river integration of DOM along the terrestrial-aquatic interface, helping to refine bottom-up carbon cycle estimates, inform upscaling of site-specific fluxes, and to track land use and climate influence on river biogeochemistry.

California

Chloroform in the hydrologic system--sources, transport, fate, occurrence, and effects on human health and aquatic organisms

Chloroform is one of the volatile organic compounds (VOCs) detected most frequently in both ground and surface water. Because it is also one of the four trihalomethanes (THMs) produced in the highest concentrations during the chlorination of drinking water and wastewater, the frequent detection of this compound in ground and surface water of the United States is presumed to be caused primarily by the input of chlorinated water to the hydrologic system. Although anthropogenic sources of the compound are substantial, they are currently estimated to constitute only 10 percent of the total global input to the hydrologic system. Natural sources of the compound include volcanic gases, biomass burning, marine algae, and soil microorganisms. Under most conditions (except in the presence of unusually high bromide concentrations), chloroform is the THM produced in the highest concentrations during chlorination. Furthermore, in most cases where more than one THM is produced from chlorination, the relative concentrations among the different compounds usually decrease with increasing bromination (chloroform > dichlorobromomethane > chlorodibromomethane > bromoform). This phenomenon is presumed to be responsible for the common observation that when more than one THM is detected during investigations of the occurrence of these compounds in the hydrologic system, this same trend is typically observed among their relative concentrations or, for a uniform reporting limit, their relative frequencies of detection. This pattern could provide a valuable means for distinguishing between chlorinated water and other potential sources of chloroform in the environment. Chloroform has been widely detected in national, regional, and local studies of VOCs in ground, surface, source, and drinking waters. Total THM (TTHM) concentrations of the compound, however, were typically less than the Maximum Contaminant Level (MCL) of 80 ?g/L (micrograms per liter) established by the U.S. Environmental Protection Agency (USEPA) for TTHMs. In the studies that compared land-use settings, frequencies of detection of chloroform were higher beneath urban and residential areas than beneath agricultural or undeveloped areas. Because chloroform is a suspected human carcinogen, its presence in drinking water is a potential human health concern. Liver damage, however, is known to occur at chloroform exposures lower than those required to cause cancer, an observation that has been considered by the USEPA as the basis for setting a new, non-zero Maximum Contaminant Level Goal of 70 ?g/L for the compound. As part of its National Water-Quality Assessment Program, the U.S. Geological Survey has been assembling and analyzing data on the occurrence of VOCs (including chloroform) in ground and surface water on a national scale from studies conducted between 1991 and the present. This report presents a summary of current (2004) information on the uses, sources, formation, transport, fate, and occurrence of chloroform, as well as its effects on human health and aquatic organisms.

Scientific Investigations Report

Blurred lines: Multiple freshwater and marine algal toxins at the land-sea interface of San Francisco Bay, California

San Francisco Bay (SFB) is a eutrophic estuary that harbors both freshwater and marine toxigenic organisms that are responsible for harmful algal blooms. While there are few commercial fishery harvests within SFB, recreational and subsistence harvesting for shellfish is common. Coastal shellfish are monitored for domoic acid and paralytic shellfish toxins (PSTs), but within SFB there is no routine monitoring for either toxin. Dinophysis shellfish toxins (DSTs) and freshwater microcystins are also present within SFB, but not routinely monitored. Acute exposure to any of these toxin groups has severe consequences for marine organisms and humans, but chronic exposure to sub-lethal doses, or synergistic effects from multiple toxins, are poorly understood and rarely addressed. This study documents the occurrence of domoic acid and microcystins in SFB from 2011 to 2016, and identifies domoic acid, microcystins, DSTs, and PSTs in marine mussels within SFB in 2012, 2014, and 2015. At least one toxin was detected in 99% of mussel samples, and all four toxin suites were identified in 37% of mussels. The presence of these toxins in marine mussels indicates that wildlife and humans who consume them are exposed to toxins at both sub-lethal and acute levels. As such, there are potential deleterious impacts for marine organisms and humans and these effects are unlikely to be documented. These results demonstrate the need for regular monitoring of marine and freshwater toxins in SFB, and suggest that co-occurrence of multiple toxins is a potential threat in other ecosystems where freshwater and seawater mix.

California

National standards and guidelines for pesticides in water, sediment, and aquatic organisms

The effects of pesticides 1 on water quality commonly are assessed by comparing measured concentrations of individual pesticide compounds in the environment with concentrations that have been determined to have potential adverse effects on humans, aquatic organisms, or other beneficial uses of water. Direct evaluation of the adverse effects of every pesticide present in a given hydrologic system is beyond the scope and budget of most water-quality studies. Many studies rely on standards or guidelines set by federal or state agencies or other institutions to indicate what concentrations may have adverse effects on human health, aquatic organisms, or wildlife. Such standards and guidelines generally are based on laboratory or field studies that document the effects of individual pesticides on specific aspects of water quality. Single-species toxicity tests (using a single species of a test organism) under various laboratory conditions are the most common type of study, whereas artificial ecosystem studies (using multiple species of a test organism) and field studies are relatively uncommon. Such studies rarely consider the effects of exposure to more than one chemical at a time. Technical information from such studies has been used by the U.S. Environmental Protection Agency (USEPA) 2 in issuing national standards, such as drinking-water regulations (for example, see USEPA 1991a) and guidelines, such as ambient water-quality criteria for the protection of human health and aquatic organisms (for example, see USEPA 1980a) to meet its statutory requirements under the Safe Drinking Water Act (SDWA) and the Clean Water Act (CWA). The Food and Drug Administration (FDA) also has used its authority under the Federal Food, Drug, and Cosmetic Act (FFDCA) to set action levels (enforceable regulatory limits) for unavoidable residues of pesticides in foods (FDA 1990).

Book chapter

Soil formation: Chapter 6

Soil formation reflects the complex interaction of many factors, among the most important of which are (i) the nature of the soil parent material, (ii) regional climate, (iii) organisms, including humans, (iv) topography and (v) time. These processes operate in Earth's critical zone; the thin veneer of our planet where rock meets life. Understanding the operation of these soil-forming factors requires an interdisciplinary approach and is a necessary predicate to charactering soil processes and functions, mitigating soil degradation and adapting soil management to environmental change. In this chapter, we discuss how these soil-forming factors operate both singly and in concert in natural and human modified environments. We emphasize the role that soil organic matter plays in these processes to provide context for understanding the benefits that it bestows on humanity.

Book chapter

Water-resources-related information for the Milwaukee Metropolitan Sewerage District planning area, Wisconsin, 1970-2002

The Milwaukee Metropolitan Sewerage District (MMSD) Corridor Study is a three-phase project designed to improve the understanding of water resources in the stream corridors of the MMSD planning area by initially compiling existing data and using the compiled information to develop 3-year baseline and long-term monitoring plans. This report is one of the products of Phase I of the Corridor Study. A literature review of surface-water-quality, surface water- quantity, and ecology studies conducted from 1970 through 2001 was completed and is summarized in this report. An inventory of Geographic Information System spatial coverages available for the MMSD planning area has been assembled. A database of water, sediment, and tissue (fish, shellfish, and others) chemistry, macroinvertebrates, fish, algae, habitat, geomorphic, and other physical and ecological data was compiled from data sets from MMSD, U.S. Geological Survey, Wisconsin Department of Natural Resources, and the U.S. Environmental Protection Agency. More than 2.7 million results are available in the MMSD Corridor Study database and the compilation of multiple datasets allows for retrieving data from a central database rather than from each of the source datasets. Data for 1970 through 2002 were collected for the 420-square-mile planning area by various agencies using different field data-collection and laboratory analysis methods. Chemical constituents and ecological components that are important to an urban setting and well represented in the database were selected for further investigation. Each constituent or component is described in this report with some or all of the following: a text summary, map of sampling locations, and in some cases median concentrations, statistical distributions of concentrations by subwatershed, table of summary statistics by subwatershed, and graphs of temporal and (or) seasonal trends. Physical data presented in the report include streamflow, stream stage, and precipitation data. Chemical indicators of water quality presented in the report include field measurements and miscellaneous constituents (pH, alkalinity, specific conductance, hardness, dissolved oxygen, biochemical oxygen demand, and chloride), sediment (total suspended solids and suspended sediment), nutrients (total nitrogen, nitrate, Kjeldahl nitrogen, total phosphorus, and dissolved phosphorus), trace elements (cadmium, mercury, copper, lead, arsenic, chromium, nickel, and zinc), pesticides (historically used pesticides and pesticides still in use), and polychlorinated biphenyls. Ecological indicators of water quality discussed in the report include community surveys of macroinvertebrates and fish, chlorophyll a concentrations, habitat assessments and channel-measurement data, and fecal coliform and E. coli bacterial counts. In addition to the compilation of the database, a major purpose of this investigation was to identify additional sampling that should be conducted under the baseline monitoring phase, which will be the second phase of the Corridor Study. Additional sampling may include: &bull; Some subwatersheds, such as those in the headwaters. &bull; Emerging contaminants such as pharmaceuticals and personal care products (PPCPs), human hormones, organic wastewater contaminants, and other constituents that result from human activity. &bull; E. coli, which can serve as an indicator of health risk to swimmers and other recreational water users. &bull; Pesticides in all media. &bull; PCBs. &bull; Trace elements in water, bed sediment, and tissues (fish, shellfish, and others). &bull; Samples during winter months or during early snowmelt episodes to address constituents such as chloride and some nutrients that have seasonal variability and that may be affected by factors such as road deicing during the winter. &bull; Samples for macroinvertebrate and fish-community data and habitat assessments. &bull; Physical data such as stream-channel cross-section profiles, bridge-scour assessments, flood-plain maps, structures, and shoreline conditions.

Wisconsin

Modulators of mercury risk to wildlife and humans in the context of rapid global change

Environmental mercury (Hg) contamination is an urgent global health threat. The complexity of Hg in the environment can hinder accurate determination of ecological and human health risks, particularly within the context of the rapid global changes that are altering many ecological processes, socioeconomic patterns, and other factors like infectious disease incidence, which can affect Hg exposures and health outcomes. However, the success of global Hg-reduction efforts depends on accurate assessments of their effectiveness in reducing health risks. In this paper, we examine the role that key extrinsic and intrinsic drivers play on several aspects of Hg risk to humans and organisms in the environment. We do so within three key domains of ecological and human health risk. First, we examine how extrinsic global change drivers influence pathways of Hg bioaccumulation and biomagnification through food webs. Next, we describe how extrinsic socioeconomic drivers at a global scale, and intrinsic individual-level drivers, influence human Hg exposure. Finally, we address how the adverse health effects of Hg in humans and wildlife are modulated by a range of extrinsic and intrinsic drivers within the context of rapid global change. Incorporating components of these three domains into research and monitoring will facilitate a more holistic understanding of how ecological and societal drivers interact to influence Hg health risks.

Ambio

Human activities cause distinct dissolved organic matter composition across freshwater ecosystems

Dissolved organic matter (DOM) composition in freshwater ecosystems is influenced by interactions between physical, chemical, and biological processes that are controlled, at one level, by watershed landscape, hydrology, and their connections. Against this environmental template, humans may strongly influence DOM composition. Yet, we lack a comprehensive understanding of DOM composition variation across freshwater ecosystems differentially affected by human activity. Using optical properties, we described DOM variation across five ecosystem groups of the Laurentian Great Lakes Region: large lakes, Kawartha Lakes, Experimental Lakes Area, urban stormwater ponds, and rivers (n = 184 sites). We determined how between ecosystem variation in DOM composition related to watershed size, land use and cover, water quality measures (conductivity, dissolved organic carbon (DOC), nutrient concentration, chlorophyll a ), and human population density. The five freshwater ecosystem groups had distinctive DOM composition from each other. These significant differences were not explained completely through differences in watershed size nor spatial autocorrelation. Instead, multivariate partial least squares regression showed that DOM composition was related to differences in human impact across freshwater ecosystems. In particular, urban/developed watersheds with higher human population densities had a unique DOM composition with a clear anthropogenic influence that was distinct from DOM composition in natural land cover and/or agricultural watersheds. This nonagricultural, human developed impact on aquatic DOM was most evident through increased levels of a microbial, humic-like parallel factor analysis component (C6). Lotic and lentic ecosystems with low human population densities had DOM compositions more typical of clear water to humic-rich freshwater ecosystems but C6 was only present at trace to background levels. Consequently, humans are strongly altering the quality of DOM in waters nearby or flowing through highly populated areas, which may alter carbon cycles in anthropogenically disturbed ecosystems at broad scales.

Global Change Biology

Occurrence and potential human-health relevance of volatile organic compounds in drinking water from domestic wells in the United States

Background As the population and demand for safe drinking water from domestic wells increase, it is important to examine water quality and contaminant occurrence. A national assessment in 2006 by the U.S. Geological Survey reported findings for 55 volatile organic compounds (VOCs) based on 2,401 domestic wells sampled during 1985–2002. Objectives We examined the occurrence of individual and multiple VOCs and assessed the potential human-health relevance of VOC concentrations. We also identified hydrogeologic and anthropogenic variables that influence the probability of VOC occurrence. Methods The domestic well samples were collected at the wellhead before treatment of water and analyzed for 55 VOCs. Results were used to examine VOC occurrence and identify associations of multiple explanatory variables using logistic regression analyses. We used a screening-level assessment to compare VOC concentrations to U.S. Environmental Protection Agency maximum contaminant levels (MCLs) and health-based screening levels. Results We detected VOCs in 65% of the samples; about one-half of these samples contained VOC mixtures. Frequently detected VOCs included chloroform, toluene, 1,2,4-trimethylbenzene, and perchloroethene. VOC concentrations generally were < 1 μg/L. One or more VOC concentrations were greater than MCLs in 1.2% of samples, including dibromochloropropane, 1,2-dichloropropane, and ethylene dibromide (fumigants); perchloroethene and trichloroethene (solvents); and 1,1-dichloroethene (organic synthesis compound). Conclusions Drinking water supplied by domestic wells is vulnerable to low-level VOC contamination. About 1% of samples had concentrations of potential human-health concern. Identifying factors associated with VOC occurrence may aid in understanding the sources, transport, and fate of VOCs in groundwater.

Environmental Health Perspectives

Enhanced microplastic fragmentation along human built structures in an urban waterway

Plastic pollution and microplastic (MP, 1 µm to 5 mm) generation are growing problems affecting the global community and a wide range of natural and disturbed environments. Urban and suburban waterways are directly impacted by plastic pollution due to their proximity to population centers and many different types single use plastic waste sources. In this study, plastic waste accumulation and fragmentation was investigated along the Cooper River in Camden County, NJ. Polymer composition was identified for individual plastic waste particles collected along the Cooper River using Fourier transform infrared (FTIR) spectrometry. Multiple human-built structures (Wallworth Lake, Evans Pond and Hopkins Pond dams) along the Cooper River were found to accumulate different types of plastic waste. The accumulation of plastic waste along these structures resulted in the initial stages of plastic fragmentation and the identification of large MP particles (1 to 5 mm). Quantitative analysis revealed that fragmented polystyrene (PS) particles constituted 82.8% of the total plastic fragments identified, most of which were identified at the Wallworth Lake dam. Many other types of fragmented plastic litter, including polyethylene and polypropylene, were identified at the Wallworth Lake dam, as well. This research demonstrates that engineered structures within urban and suburban aquatic ecosystems serve as significant aggregators of plastic debris, thereby catalyzing its breakdown into microplastics. Considering the escalating ecological and human health ramifications of microplastic proliferation, the fragmentation of plastic waste in an urban and suburban waterway observed in this study can also result in potentially toxic smaller MP particles, and increased exposure to aquatic organisms and humans.

New Jersey

Septic systems and rainfall influence human fecal markers and indicator organisms occurrence in private wells in southeastern Pennsylvania

In the United States approximately 48 million people are served by private wells. Unlike public water systems, private well water quality is not monitored and there are few studies on the extent and sources of contamination of private wells. We extensively investigated five private wells to understand the variability in microbial contamination, the role of septic systems as sources of contamination, and the effect of rainfall on well water quality. From 2016-2017, weekly or biweekly samples (n= 105) were collected from 5 private wells in rural Pennsylvania. Samples were tested for general water quality parameters, conventional and sewage-associated microbial indicators, and human pathogens. Total coliforms, human Bacteroides (HF183), and pepper mild mottle virus were detected at least once in all wells. Regression revealed significant relationships between HF183 and rainfall 8-14 days prior to sampling and between total coliforms and rainfall 8-14 or 0-14 days prior to sampling. Dye tracer studies at 3 wells confirmed the impact of household septic systems on well contamination. Microbiological measurements, chemical water quality data, and dye tracer tests provide evidence of human fecal contamination in the private wells studied, suggesting that household septic systems are the source of this contamination.

Pennsylvania

Continuous-flow centrifugation to collect suspended sediment for chemical analysis

Recent advances in suspended-sediment monitoring tools and surrogate technologies have greatly improved the ability to quantify suspended-sediment concentrations and to estimate daily, seasonal, and annual suspended-sediment fluxes from rivers to coastal waters. However, little is known about the chemical composition of suspended sediment, and how it may vary spatially between water bodies and temporally within a single system owing to climate, seasonality, land use, and other natural and anthropogenic drivers. Many water-quality contaminants, such as organic and inorganic chemicals, nutrients, and pathogens, preferentially partition in sediment rather than water. Suspended sediment-bound chemical concentrations may be undetected during analysis of unfiltered water samples, owing to small water sample volumes and analytical limitations. Quantification of suspended sediment‑bound chemical concentrations is needed to improve estimates of total chemical concentrations, chemical fluxes, and exposure levels of aquatic organisms and humans in receiving environments. Despite these needs, few studies or monitoring programs measure the chemical composition of suspended sediment, largely owing to the difficulty in consistently obtaining samples of sufficient quality and quantity for laboratory analysis. A field protocol is described here utilizing continuous‑flow centrifugation for the collection of suspended sediment for chemical analysis. The centrifuge used for development of this method is small, lightweight, and portable for the field applications described in this protocol. Project scoping considerations, deployment of equipment and system layout options, and results from various field and laboratory quality control experiments are described. The testing confirmed the applicability of the protocol for the determination of many inorganic and organic chemicals sorbed on suspended sediment, including metals, pesticides, polycyclic aromatic hydrocarbons, and polychlorinated biphenyls. The particle-size distribution of the captured sediment changes to a more fine-grained sample during centrifugation, and the necessity to account for this change when extrapolating chemical concentrations on the centrifuged sediment sample to the environmental water system is discussed. The data produced using this method will help eliminate a data gap of suspended sediment-bound chemical concentrations, and will support management decisions, such as chemical source-control efforts or in-stream restoration activities. When coupled with streamflow and sediment flux data, it will improve estimates of riverine chemical fluxes, and will aid in assessing the importance and impacts of suspended sediment-bound chemicals to downstream freshwater and coastal marine ecosystems.

Techniques and Methods