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At least 163 records · Page 9Linked to original sources
USGS1999

Identification of duck plague virus by polymerase chain reaction

A polymerase chain reaction (PCR) assay was developed for detecting duck plague virus. A 765-bp EcoRI fragment cloned from the genome of the duck plague vaccine (DP-VAC) virus was sequenced for PCR primer development. The fragment sequence was found by GenBank alignment searches to be similar to the 3′ ends of an undefined open reading frame and the gene for DNA polymerase protein in other herpesviruses. Three of four primer sets were found to be specific for the DP-VAC virus and 100% (7/7) of field isolates but did not amplify DNA from inclusion body disease of cranes virus. The specificity of one primer set was tested with genome templates from other avian herpesviruses, including those from a golden eagle, bald eagle, great horned owl, snowy owl, peregrine falcon, prairie falcon, pigeon, psittacine, and chicken (infectious laryngotracheitis), but amplicons were not produced. Hence, this PCR test is highly specific for duck plague virus DNA. Two primer sets were able to detect 1 fg of DNA from the duck plague vaccine strain, equivalent to five genome copies. In addition, the ratio of tissue culture infectious doses to genome copies of duck plague vaccine virus from infected duck embryo cells was determined to be 1:100, making the PCR assay 20 times more sensitive than tissue culture for detecting duck plague virus. The speed, sensitivity, and specificity of this PCR provide a greatly improved diagnostic and research tool for studying the epizootiology of duck plague.

Avian Diseases↗
USGS2012

Plague

Plague offers readers an overview of this highly complex disease caused by the bacteria Yersinia pestis . The history of the disease, as well as information about Yersinia pestis and its transmission by fleas, is described. The section Geographic Distribution presents areas of the world and United States where plague occurs most commonly in rodents and humans. Species Susceptibility describes infection and disease rates in rodents, humans, and other animals. Disease Ecology considers the complex relationship among rodents, domestic and wild animals, and humans and explores possible routes of transmission and maintenance of the organism in the environment. The effects of climate change, the potential for Y. pestis to be used as a bioweapon, and the impact of plague on conservation of wildlife are considered in Points to Ponder . Disease Prevention and Control outlines methods of prevention and treatment including vaccination for prairie dogs and black-footed ferrets. A glossary of technical terms is included. Tonie E. Rocke, the senior author and an epizootiologist at the USGS National Wildlife Health Center (NWHC), is a prominent researcher on oral vaccination of prairie dogs to prevent plague. She is currently working to transfer her success in the laboratory to the field to control plague in prairie dogs. Rachel C. Abbott, a biologist at the NWHC, is assisting Dr. Rocke in this process and will coordinate field trials of the vaccine. Milt Friend, first director of the NWHC, wrote the foreword. Plague is intended for scholars and the general public. The material is presented in a simple, straightforward manner that serves both audiences. Numerous illustrations and tables provide easily understood summaries of key points and information.

Circular↗
USGS2026

Columbia Basin pygmy rabbit recovery planning through structured decision making

The endangered Columbia Basin pygmy rabbit (CBPR) faces multiple threats, particularly increasing risk of larger and more intense wildfires due to climate change, emerging disease, and sagebrush habitat loss due to agriculture and development. Through the use of conservation breeding, the CBPR wild population grew from a low of 16 individuals captured in 2001 to over 100 individuals in two subpopulations in 2024. However, these subpopulations are geographically proximate, with potential risk that both subpopulations could be affected by a single wildfire or disease event. Additionally, a succession of setbacks in the breeding program has prompted a natural re-evaluation point for the CBPR conservation program. We undertook a structured decision-making (SDM) process with participants from both Washington Department of Fish and Wildlife (WDFW) and US Fish and Wildlife Service (USFWS) to develop a strategy that is sustainable and implementable for guiding management in the coming decades across the range of the CBPR, taking into account changing conditions and updated information. A population model that incorporated both demographic and high impact event uncertainties was developed to test how alternative strategies – defined by conservation breeding program, vaccination, and translocation components – affect CBPR population growth and cost objectives. Based on analysis of the model results, we identified the following actions that appear to have the greatest potential to allow WDFW and USFWS to meet their conservation objectives for CBPR: Continue conservation breeding program, and possibly expand to include an island subpopulation (an isolated, unfenced area that can serve as a source for rabbits while requiring fewer management inputs) Continue Rabbit Hemorrhagic Disease Virus (RHDV2) annual vaccinations in both breeding and wild populations When juveniles are available for translocation, prioritize recovery areas that are in the establishing phase In addition, while not analyzed explicitly in the model, discussions during the SDM process led to the identification of the following actions, which the group considered to have potential to benefit the CBPR either directly or indirectly: Increase the amount of suitable habitat available to pygmy rabbits Increase protections for existing and potential recovery areas Design future monitoring to better estimate survival and reproduction, with an emphasis on understanding how these vital rates vary between wild and semi-captive individuals, between vaccinated and unvaccinated individuals, and as a function of habitat factors

BioRxiv↗
USGS2011

Clinical pathology results from cranes with experimental West Nile Virus infection

Sandhill cranes ( Grus canadensis ) were vaccinated for and then challenged with West Nile virus. Resulting titers demonstrated protection in the vaccinated-challenged cranes as compared to the unvaccinated-challenged cranes. Clinical pathology results showed challenged cranes, whether vaccinated or not, had a decrease in their hematocrits and an elevation of 2.5-fold in their white blood cell counts as compared to unchallenged control sandhill cranes. No differences were apparent in the differential counts of heterophils and lymphocytes.

Proceedings of the Association of Avian Veterinari↗
USGS2013

An ecological perspective on the changing face of Brucella abortus in the western United States

After a hiatus during the 1990s, outbreaks of Brucella abortus in cattle are occurring more frequently in some of the western states of the United States, namely, Montana, Wyoming and Idaho. This increase is coincident with increasing brucellosis seroprevalence in elk (Cervus elaphus), which is correlated with elk density. Vaccines are a seductive solution, but their use in wildlife systems remains limited by logistical, financial, and scientific constraints. Cattle vaccination is ongoing in the region. Livestock regulations, however, tend to be based on serological tests that test for previous exposure and available vaccines do not protect against seroconversion. The authors review recent ecological studies of brucellosis, with particular emphasis on the Greater Yellowstone Area, and highlight the management options and implications of this work, including the potential utility of habitat modifications and targeted hunts, as well as scavengers and predators. Finally, the authors discuss future research directions that will help us to understand and manage brucellosis in wildlife.

OIE Revue Scientifique et Technique↗
USGS2009

Characterization of the interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential regulation of duplicated genes

The genes encoding the type I and type II interferons (IFNs) have previously been identified in rainbow trout and their proteins partially characterized. These previous studies reported a single type II IFN (rtIFN-??) and three rainbow trout type I IFN genes that are classified into either group I (rtIFN1, rtIFN2) or group II (rtIFN3). In this present study, we report the identification of a novel IFN-?? gene (rtIFN-??2) and a novel type I group II IFN (rtIFN4) in homozygous rainbow trout and predict that additional IFN genes or pseudogenes exist in the rainbow trout genome. Additionally, we provide evidence that short and long forms of rtIFN1 are actively and differentially transcribed in homozygous trout, and likely arose due to alternate splicing of the first exon. Quantitative reverse transcriptase PCR (qRT-PCR) assays were developed to systematically profile all of the rainbow trout IFN transcripts, with high specificity at an individual gene level, in na??ve fish and after stimulation with virus or viral-related molecules. Cloned PCR products were used to ensure the specificity of the qRT-PCR assays and as absolute standards to assess transcript abundance of each gene. All IFN genes were modulated in response to Infectious hematopoietic necrosis virus (IHNV), a DNA vaccine based on the IHNV glycoprotein, and poly I:C. The most inducible of the type I IFN genes, by all stimuli tested, were rtIFN3 and the short transcript form of rtIFN1. Gene expression of rtIFN-??1 and rtIFN-??2 was highly up-regulated by IHNV infection and DNA vaccination but rtIFN-??2 was induced to a greater magnitude. The specificity of the qRT-PCR assays reported here will be useful for future studies aimed at identifying which cells produce IFNs at early time points after infection. ?? 2008 Elsevier Ltd.

Fish and Shellfish Immunology↗
USGS2022

Plague circulation in small mammals elevates extinction risk for the endangered Peñasco least chipmunk

Wildlife diseases are a major concern for species survival around the world. Vector-borne diseases, in particular, are problematic for both humans and wildlife. Plague is an introduced disease to North America where many species have low natural resistance to infection by the causative bacterium, Yersinia pestis . Plague in the United States is often associated with large-scale epizootic events that impact rodent populations. However, we need more information regarding the effects of plague at enzootic levels and in species that rarely if ever experience epizootic events. New Mexico is a state that experiences regular epizootic plague events in several species of rodents but comparatively less is known about enzootic plague impacts within rodent communities in New Mexico. The Peñasco least chipmunk ( Neotamias minimus atristriatus ) is state-listed as endangered in New Mexico and is a candidate for listing under the federal Endangered Species Act. It likely has been extirpated from the southern portion of its range and is now observed only at high elevations in isolated areas in the White Mountains. We evaluated plague presence in the more abundant small mammal species associated with these chipmunks. We used an F1 antigen plague-specific vaccine and a placebo injection to test whether plague was present and reducing survival in western deer mouse ( Peromyscus sonoriensis ) and vole ( Microtus spp.) populations in the Lincoln National Forest. The effect of plague on survival differed among years and sites. Monthly apparent survival was up to 60 % higher for rodents given the vaccine compared to those given a placebo. Our results suggest that plague is likely impacting the small mammal community in the Lincoln National Forest and is even present at higher elevation sites where the Peñasco least chipmunk was most recently extirpated. Because least chipmunks are highly susceptible to plague, our results suggest that this disease may be an important factor in the population decline of the Peñasco least chipmunk.

New Mexico↗
USGS2010

Characterization of a herpes virus isolated from domestic geese in Australia

A herpesvirus (GHV 552/89) associated with high mortality in a flock of domestic geese in Australia was compared with duck virus enteritis (DVE) herpesvirus by cross-protection studies in domestic geese, Muscovy ducks and commercial Pekin ducks. In DVE-vaccinated geese, Muscovy ducks and Pekin ducks, mortality levels of 100, 50 and 0%, respectively, were recorded following challenge with GHV 552/89. Conversely, in geese, Muscovy ducks and Pekin ducks immunized with inactivated GHV 552/89, 100% mortality was observed in the geese and Muscovy ducks, and 80% in the Pekin ducks following challenge with DVE virus. The isolate was also compared with six other avian herpesviruses using cross-neutralization tests in cell cultures. No detectable cross-neutralization occurred with any of the avian herpesviruses tested. Further characterization of GHV 552/89 was undertaken by comparing its genome with strains of DVE herpesvirus using restriction endonuclease analysis of the viral DNA and a polymerase chain reaction (PCR) test. Following digestion with HindIII, the DNA fragment pattern of GHV 552/89 was found to be completely different from the DVE viruses. Similarities were found between the digestion patterns of a UK and a US DVE isolate, but both were distinguishable from a UK vaccine strain. The results of the PCR analysis and comparison using two DVE-specific primer sets did not produce specific amplification products of expected molecular weights (603 and 446 base pairs) from the GHV 552/89 genome. The PCR products derived from the DVE strains were similar to those derived from the DVE control DNA. From the results of this study, it is concluded that the goose herpesvirus GHV 552/89 is antigenically and genomically distinct from DVE herpesvirus.

Avian Pathology↗
USGS2021

Epizootic plague in prairie dogs: Correlates and control with deltamethrin

The plague bacterium, Yersinia pestis , is a generalist pathogen of flea (Siphonaptera) vectors and mammalian hosts. In colonies of prairie dogs (PDs, Cynomys spp.), Y. pestis causes occasional epizootics, killing ≥90% of PDs within weeks to several months. We evaluated the effectiveness of deltamethrin, a pyrethroid insecticide, as a tool for preventing plague epizootics among three PD species. Specifically, we studied PD population growth on paired plots treated with deltamethrin for flea control or left untreated as baselines. We also evaluated PD population growth relative to flea abundance and PD density. All epizootics occurred on nontreated plots. Epizootics occurred on plots with very low PD densities as well as high densities. Mean population change, assessed by comparing visual counts of PDs in years before and during epizootics, was +88% for treated plots and −97% for nontreated plots. For comparison, an experimental oral vaccine against plague had an average change in population index or estimate during epizootics of −69% on vaccine plots compared with −83% for associated nontreated (placebo) plots. In our study and on plots not treated with deltamethrin, PD population growth was negatively correlated with flea abundance in the year before the epizootic, lending support to the hypothesis that flea abundance plays a critical role in plague transmission under natural conditions. Generally speaking, deltamethrin is a highly effective tool for plague management on PD colonies. That said, continued study is needed to refine deltamethrin treatments and to develop a more integrated strategy for plague management.

Vector-Borne and Zoonotic Diseases↗
USGS2010

Influence of fish oils in production catfish feeds on selected disease resistance factors

Healthy channel calfish, Ictalurus punctatus (mean weight: 62.5g), were fed two production feeds that were identical except for the lipid portion, which was either catfish oil or menhaden oil. Fish were held at 18 or 28°C and fed the experimental feeds for four monlhs. A subsample of fish from each of the four treatments was then used to measure scrum antibody titers to Edwardsiella ictaluri , macrophage phagocytic index, phagocytic capacity, and production of intracellular and extracellular superoxide anion. The remaining fish were vaccinated with an E. icraluri bacterin, held for 18-24 days, and the assays were repeated. Fatty acid profiles of the diets indicated catfish oil had a higher percentage of monounsaturated fatty acids and slightly higher n-6 fatty acids. The menhaden oil diet had a higher percentage of the n-3 fatty acids. At 28°C, weight gain and feed conversion were slightly higher in calfish fed the feed containing menhaden oil. Weight gains and feed conversion were similar in fish maintained on the two feeds at 18°C. At the lower temperature, fish fed the diet containing menhaden oil appeared to have enhanced disease resistance factors, particularly phagocytic capacity. However, at 28°C, fish fed the diet containing catfish oil had enhanced phagocytic ability and intracellular superoxide production. Antibody titers were not sigmficantly (P > 0.05) affected by dietary lipid in this study. Vaccination amplified phagocytic ability in both diets at both temperatures and intensified the production of superoxide anion at 28°C.

Journal of Applied Aquaculture↗
USGS2018

The impact of surveillance and control on highly pathogenic avian influenza outbreaks in poultry in Dhaka division, Bangladesh

In Bangladesh, the poultry industry is an economically and socially important sector, but it is persistently threatened by the effects of H5N1 highly pathogenic avian influenza. Thus, identifying the optimal control policy in response to an emerging disease outbreak is a key challenge for policy-makers. To inform this aim, a common approach is to carry out simulation studies comparing plausible strategies, while accounting for known capacity restrictions. In this study we perform simulations of a previously developed H5N1 influenza transmission model framework, fitted to two separate historical outbreaks, to assess specific control objectives related to the burden or duration of H5N1 outbreaks among poultry farms in the Dhaka division of Bangladesh. In particular, we explore the optimal implementation of ring culling, ring vaccination and active surveillance measures when presuming disease transmission predominately occurs from premises-to-premises, versus a setting requiring the inclusion of external factors. Additionally, we determine the sensitivity of the management actions under consideration to differing levels of capacity constraints and outbreaks with disparate transmission dynamics. While we find that reactive culling and vaccination policies should pay close attention to these factors to ensure intervention targeting is optimised, across multiple settings the top performing control action amongst those under consideration were targeted proactive surveillance schemes. Our findings may advise the type of control measure, plus its intensity, that could potentially be applied in the event of a developing outbreak of H5N1 amongst originally H5N1 virus-free commercially-reared poultry in the Dhaka division of Bangladesh.

Dhaka Division↗
USGS2013

Mapping monkeypox transmission risk through time and space in the Congo Basin

Monkeypox is a major public health concern in the Congo Basin area, with changing patterns of human case occurrences reported in recent years. Whether this trend results from better surveillance and detection methods, reduced proportions of vaccinated vs. non-vaccinated human populations, or changing environmental conditions remains unclear. Our objective is to examine potential correlations between environment and transmission of monkeypox events in the Congo Basin. We created ecological niche models based on human cases reported in the Congo Basin by the World Health Organization at the end of the smallpox eradication campaign, in relation to remotely-sensed Normalized Difference Vegetation Index datasets from the same time period. These models predicted independent spatial subsets of monkeypox occurrences with high confidence; models were then projected onto parallel environmental datasets for the 2000s to create present-day monkeypox suitability maps. Recent trends in human monkeypox infection are associated with broad environmental changes across the Congo Basin. Our results demonstrate that ecological niche models provide useful tools for identification of areas suitable for transmission, even for poorly-known diseases like monkeypox.

PLoS ONE↗
USGS1998

Control of furunculosis and enteric redmouth disease in sea-run Atlantic salmon broodstock in the Connecticut and Merrimack Rivers

Adult sea‐run Atlantic salmon Salmo salar captured and transported to Richard Cronin National Salmon Station (Sunderland, Massachusetts), Nashua National Fish Hatchery (Nashua, New Hampshire), and Whittemore State Fish Hatchery (Waterford, Connecticut) during 1986–1992 were treated with oxolinic acid and a bacterin. The bacterin was developed against furunculosis and enteric redmouth disease. Among the 2,552 fish that were treated since 1986, 362 died and 65 (18%) of those fish had furunculosis. Among 206 untreated fish that were maintained as controls, 109 died and 63 (57.8%) had furunculosis. The reduction in mortality could not be attributed to either vaccine or antibiotic alone without further study. A 3‐year study was designed to investigate if adult Atlantic salmon, undergoing the stress of migration, handling, and spawning, could mount a protective humoral immune response. Although the salmon were able to produce an agglutinin response, evidence was not found for production of a protective humoral response by these vaccinated Atlantic salmon.

Progressive Fish-Culturist↗
USGS2021

Genetic sequencing of Attwater's prairie chicken avian poxvirus and evaluation of its potential role in reticuloendotheliosis virus outbreaks

Efforts to breed Attwater's prairie chickens (APC; Tympanuchus cupido attwateri ) in captivity to supplement wild populations of this endangered bird have been negatively affected by infections with Avipoxvirus and reticuloendotheliosis virus (REV). Because REV can be integrated into the genome of fowlpox virus (FPV) and may be transmitted in that manner, identifying the source of avipox disease in APC is important to mitigate the impact of this virus. Tissue samples from APC were collected from breeding programs in Texas from 2016 to 2020. These samples consisted of 11 skin lesions and three internal organs from a total of 14 different birds that died of unknown causes or were euthanized. Avipoxvirus was detected by PCR and isolation in embryonating chicken eggs in all skin lesion samples but was not detected in internal organs. Using sequence analysis of FPV polymerase and 4b genes, we determined that 10 out of 11 Avipoxvirus detections resided within the fowlpox clade and a single sample resided within the canarypox clade. REV sequences were detected in all FPV positive samples and in all internal organ tissues but were not detected in the sample matching the canarypox clade. Analysis of REV sequences and PCR detection showed the REV infecting APC was consistent with REV-A and had little variability on analysis of the U3 region of the long terminal repeat. The results of this study indicate control of REV in APC breeding colonies may benefit by a vaccination program targeting FPV and REV. However, a commercially available vaccine for REV is not available at this time.

Avian Diseases↗
USGS2020

Clinical presentation and serological responses to natural outbreaks of rabies in a captive colony of common vampire bats

We report mortality events in a group of 123 common vampire bats ( Desmodus rotundus ) captured in México and housed for a rabies vaccine efficacy study in Madison, Wisconsin. Bat mortalities occurred in México and Wisconsin, but rabies cases reported herein are only those that occurred after arrival in Madison (n = 15). Bats were confirmed positive for rabies virus (RABV) by the direct fluorescent antibody test. In accordance with previous reports, we observed long incubation periods (more than 100 days), variability in clinical signs prior to death, excretion of virus in saliva, and changes in rabies neutralizing antibody (rVNA) titers post-infection. We observed that the furious form of rabies (aggression, hyper-salivation, and hyper-excitability) manifested in three bats, which has not been reported in vampire bat studies since 1936. RABV was detected in saliva of 5/9 bats, 2–5 days prior to death, but was not detected in four of those bats that had been vaccinated shortly after exposure. Bats from different capture sites were involved in two separate outbreaks, and phylogenetic analysis revealed differences in the glycoprotein gene sequences of RABV isolated from each event, indicating that two different lineages were circulating separately during capture at each site.

Tropical Medicine and Infectious Disease↗
USGS1985

Immunizing Canada geese against avian cholera

A small flock of captive giant Canada geese were vaccinated with the experimental bac- terin in Nebraska to test its efficacy under field conditions. Only 2 of 157 vaccinates died from avian cholera during an annual spring die-off.

Wildlife Society Bulletin↗
USGS1989

The impact of eastern equine encephalitis virus on efforts to recover the endangered whooping crane

The whooping crane (Grus americana), although never abundant in North America, became endangered primarily because of habitat modification and destruction. To help recovery, a captive propagation and reintroduction program was initiated at the Patuxent Wildlife Research Center (PWRC) in 1966. However, in 1984, 7 of 39 whooping cranes at PWRC died from infection by eastern equine encephalitis (EEE) virus, an arbovirus that infects a wide variety of indigenous bird species, although mortality is generally restricted to introduced birds. Following identification of the aetiological agent, surveillance and control measures were implemented, including serological monitoring of both wild and captive birds for EEE viral antibody and assay of locally-trapped mosquitoes for virus. In addition, an inactivated EEE virus vaccine developed for use in humans was evaluated in captive whooping cranes. Results so far suggest that the vaccine will afford protection to susceptible birds.

Book chapter↗
USGS2016

Hematology results from experimental exposure of sandhill cranes to West Nile virus

West Nile virus is a deadly virus for young cranes. In testing two different vaccines on both adult sandhill cranes (Grus canadensis), we discovered that some blood parameters are altered by exposure to the virus. White blood cell counts were the most obvious, and may be used as an indicator of West Nile virus exposure in cranes. Other hematology and serum chemistry results were studied and only hematocrit, percent heterophils, and percent lymphocytes were of interest, along with the already published information on titers encountered in experimental infections. Clinical pathology results showed challenged cranes, whether vaccinated or not, had a decrease in their hematocrits and an elevation of 2.5-fold in their white blood cell counts as compared to unchallenged control sandhill cranes. No differences were apparent in the differential counts of heterophils and lymphocytes. Our work would suggest that a combination of white blood cell counts and antibody titers can be used to diagnose and assess the severity of West Nile virus infections in cranes.

Conference Paper↗