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Geohydrologic and drill-hole data for test well USW H-4, Yucca Mountain, Nye County, Nevada

Data are presented on drilling operations, lithology, geophysical well logs, sidewall-core samples, water-level monitoring, pumping tests, injection tests, radioactive-tracer borehole flow survey, and water chemistry for test well USW H-4. The well is one of a series of test wells drilled in the southwestern part of the Nevada Test Site, Nye County, Nevada, in cooperation with the U.S. Department of Energy. These test wells are part of the Nevada Nuclear Waste Storage Investigations to identify sites for storage of high-level radioactive wastes. Test well USW H-4 was drilled in ash-flow tuff to a total depth of 1,219 meters. Depth to water below land surface was 519 meters or at an altitude of 730 meters above sea level. After test pumping at a rate of 17.4 liters per second for approximately 9 days, the drawdown was 4.85 meters. A radioactive borehole-flow survey indicated that the Bullfrog Member was the most productive geologic unit, producing 36.5 percent of the water in the well. The second most productive geologic unit was the Tram Member, which produced 32 percent of the water. The water in test well USW H-4 is predominantly a soft, sodium bicarbonate type of water typical of water produced in tuffaceous rocks in southern Nevada. (USGS)

Open-File Report

Chemical analysis of water samples and geophysical logs from cored test holes drilled in the central Oklahoma Aquifer, Oklahoma

Chemical analyses of water from eight test holes and geophysical logs for nine test holes drilled in the Central Oklahoma aquifer are presented. The test holes were drilled to investigate local occurrences of potentially toxic, naturally occurring trace substances in ground water. These trace substances include arsenic, chromium, selenium, residual alpha-particle activities, and uranium. Eight of the nine test holes were drilled near wells known to contain large concentrations of one or more of the naturally occurring trace substances. One test hole was drilled in an area known to have only small concentrations of any of the naturally occurring trace substances. Water samples were collected from one to eight individual sandstone layers within each test hole. A total of 28 water samples, including four duplicate samples, were collected. The temperature, pH, specific conductance, alkalinity, and dissolved-oxygen concentrations were measured at the sample site. Laboratory determinations included major ions, nutrients, dissolved organic carbon, and trace elements (aluminum, arsenic, barium, beryllium, boron, cadmium, chromium, hexavalent chromium, cobalt, copper, iron, lead, lithium, manganese, mercury, molybdenum, nickel, selenium, silver, strontium, vanadium and zinc). Radionuclide activities and stable isotope (5 values also were determined, including: gross-alpha-particle activity, gross-beta-particle activity, radium-226, radium-228, radon-222, uranium-234, uranium-235, uranium-238, total uranium, carbon-13/carbon-12, deuterium/hydrogen-1, oxygen-18/oxygen-16, and sulfur-34/sulfur-32. Additional analyses of arsenic and selenium species are presented for selected samples as well as analyses of density and iodine for two samples, tritium for three samples, and carbon-14 for one sample. Geophysical logs for most test holes include caliper, neutron, gamma-gamma, natural-gamma logs, spontaneous potential, long- and short-normal resistivity, and single-point resistance. Logs for test-hole NOTS 7 do not include long- and short-normal resistivity, spontaneous-potential, or single-point resistivity. Logs for test-hole NOTS 7A include only caliper and natural-gamma logs.

Oklahoma

Aquifer test at well SMW-1 near Moenkopi, Arizona

The Hopi villages of Lower Moencopi and Upper Moenkopi are on the Hopi Indian Reservation south of Tuba City in northern Arizona. These adjacent Hopi villages, located west and north of the confluence of Pasture Canyon Wash and Moenkopi Wash, are dependent on groundwater withdrawals from three wells that penetrate the N aquifer and from two springs that discharge from the N aquifer. The N aquifer is the principal aquifer in this region of northern Arizona and is composed of thick beds of sandstone between less permeable layers of siltstone and mudstone. The fine-grained character of the N aquifer inhibits rapid movement of water and large yields to wells; however, the aquifer is moderately productive at yields generally less than 25 gallons per minute in the study area. In recent years, the water level has declined in the three public-supply wells and the flow from the springs has decreased, causing concern that the current water supply will not be able to accommodate peak demand and allow for residential and economic growth. In addition to the challenge imposed by declining groundwater levels, the water-supply wells and springs are located about 2 miles downgradient from the Tuba City Landfill site where studies are ongoing to determine if uranium and other metals in groundwater beneath the landfill are higher than regional concentrations in the N aquifer. In August 2008, the U.S. Geological Survey, in cooperation with the Hopi Tribe, conducted an aquifer test on well SMW-1, designed to help the Hopi Tribe determine the potential yield and water quality of the N aquifer south of Moenkopi Wash as a possible source of additional water supply. Well SMW-1 was drilled south of Moenkopi Wash to a depth of 760 feet below land surface before being backfilled and cased to about 300 feet. The well penetrates, in descending order, the Navajo Sandstone and the Kayenta Formation, both units of the N aquifer. The pre-test water level in the well was 99.15 feet below land surface. A 9.25-hour step-drawdown test and a 72-hour constant-rate test followed by recovery tests were used to investigate the performance of the test well and to estimate the transmissivity and potential yield of the N aquifer south of Moenkopi Wash. The test data were analyzed using the Cooper-Jacob method adjusted for confined conditions, the Papadopulos-Cooper method that accounts for wellbore storage, and the Theis method on the recovery data. Results of the tests indicate that in the vicinity of the well, the N aquifer has a transmissivity of about 50 feet squared per day. The test well, as completed, should yield about 15 gallons per minute with about 75 feet of drawdown (less than half of the available saturated thickness of the aquifer at the well).

Arizona

Groundwater levels and water quality during a 96-hour aquifer test in Pickaway County, Ohio, 2012

During October–November 2012, a 96-hour aquifer test was performed at a proposed well field in northern Pickaway County, Ohio, to investigate groundwater with elevated nitrate concentrations. Earlier sampling done by the City of Columbus revealed that some wells had concentrations of nitrate that approached 10 milligrams per liter (mg/L), whereas other wells and the nearby Scioto River had concentrations from 2 to 6 mg/L. The purpose of the current test was to examine potential changes in water quality that may be expected if the site was developed into a public water-supply source; therefore, water-transmitting properties determined during a previous test were not determined a second time. Before and during the test, water-level data and water-quality samples were obtained from observation wells while a test production well was pumped at 1,300 gallons per minute. Before the test, local groundwater levels indicated that groundwater was being discharged to the nearby Scioto River, but during the test, the stream was losing streamflow owing to infiltration. Water levels declined in the pumping well, in adjacent observation wells, and in a nearby streambed piezometer as pumping commenced. The maximum drawdown in the pumping well was 29.75 feet, measured about 95 hours after pumping began. Water-quality data, including analyses for field parameters, major and trace elements, nutrients, and stable isotopes of oxygen and nitrogen in nitrate, demonstrated only small variations before and during the test. Concentrations of nitrate in five samples from the pumping well ranged from about 5.10 to 5.42 mg/L before and during the test, whereas concentrations of nitrate in five samples on or about the same sampling dates and times at a monitoring site on the Scioto River adjacent to the pumping well ranged from 3.46 to 4.97 mg/L. Water from two nearby observation wells had nitrate concentrations approaching 10 mg/L, which is the U.S. Environmental Protection Agency’s Maximum Contaminant Level for nitrate. Analysis of isotopes of oxygen and nitrogen in nitrate indicated that the source of nitrate is most likely soil nitrogen and fertilizer, with some denitrification and (or) mixing with some manure and septic waste derived from upstream wastewater-treatment facilities.

Ohio

Groundwater hydrology, groundwater and surface-water interactions, aquifer testing, and groundwater-flow simulations for the Fountain Creek alluvial aquifer, near Colorado Springs, Colorado, 2018–20

From 2018 through 2020, the U.S. Geological Survey, in cooperation with the Air Force Civil Engineering Center, conducted an integrated study of the Fountain Creek alluvial aquifer located near Colorado Springs, Colorado. The objective of the study was to characterize hydrologic conditions for the alluvial aquifer pertinent to the potential for transport of solutes. Specific goals of this report were to characterize the groundwater hydrology of the area, to quantify groundwater and surface-water interactions, to estimate hydraulic properties of the aquifer using aquifer testing, and to complete numerical simulations of groundwater flow. Synoptic groundwater-level elevation measurements completed throughout this study, and as part of other U.S. Geological Survey programs between 1994 and 2020, indicate groundwater-level elevations fluctuate on annual and interannual timeframes. Groundwater-level fluctuations likely were caused by temporally variable groundwater recharge and discharge components in the area, with many wells showing maximum groundwater-level elevations during the winter months (November through March). From an interannual perspective, groundwater-level fluctuations appear to have reached maximum values during 2000 to 2003, decreased during 2003 to 2006, and remained relatively constant since that time, with the exception of several wells which have displayed rising groundwater-level elevations since 2018. Spatial evaluation of groundwater-level elevations indicates groundwater flow is generally from northeast to southwest within the vicinity of several alluvial paleochannels occurring along the northeastern margin of the aquifer. Within the center of the aquifer along Fountain Creek, groundwater flow is generally from north to south, approximately paralleling surface-water flow. To quantitatively understand the potential effect of groundwater recharge and groundwater pumping on fluctuations in groundwater-level elevation, a statistical transfer-function-noise model was applied. Results of the statistical model indicate throughout most of the aquifer, fluctuations were primarily the result of recharge seasonality. In the main stem of the aquifer where groundwater pumping wells were more concentrated, however, groundwater-level elevation fluctuations were also attributable to groundwater pumping through time. Three-dimensional evaluation of the aquifer geometry near Fountain Creek was combined with synoptic streamflow measurement and accounting of stream gains and losses to evaluate groundwater and surface-water interactions in the study area. Streamflow gain or loss calculations indicate Fountain Creek both gains from and loses flow to the alluvial aquifer, and gaining or losing reaches of the stream may be partially controlled by the depth to bedrock near the stream. Reaches with streamflow gains tend to coincide with areas where the estimated depth to bedrock is decreasing, meaning the alluvial aquifer is likely thinning in these areas and groundwater-flow paths may be converging and discharging groundwater to the stream. Losing reaches tended to coincide with locally greater depth to bedrock where the alluvial aquifer is likely thicker and has greater storage potential for surface water lost from Fountain Creek. Results of aquifer testing indicate hydraulic conductivity, estimated from slug tests and single-well pumping tests, ranged from 0.32 to 1,410 feet per day (ft/d) and 4.13 to 664 ft/d, respectively. These results are similar to the range of values from previous aquifer tests in the study area. Hydraulic conductivities from aquifer testing for this study were generally greater than the estimates of previous slug tests and had a mean value less than the estimates from previous pumping tests. Spatial evaluation of aquifer testing results indicates hydraulic conductivity tends to be greater in the main stem of the alluvial aquifer and lower in paleochannels upgradient from the main stem of the aquifer. The spatial variation in hydraulic conductivity may be attributed to the geomorphologic processes that formed the alluvial aquifer. Compacted sediment in the paleochannels has not been potentially transported sufficient distance to cause grain-size sorting, resulting in a poorly sorted deposit and lower hydraulic conductivities. In the central portion of the alluvial aquifer, near Fountain Creek, the sediments have been transported farther from their source areas and are likely better sorted, removing finer grained sediments that would cause lower hydraulic conductivity. A numerical groundwater-flow model was calibrated for the Fountain Creek alluvial aquifer for 2000–19 to simulate water-budget components, groundwater-flow directions, and groundwater-flow paths. The model simulated precipitation recharge, groundwater and surface-water interactions, evapotranspiration, high-volume groundwater pumping by pumping wells, and external inflows and outflows occurring along the boundaries of the alluvial aquifer. Model calibration was completed using manual and automated approaches, the latter of which assisted in quantifying model results sensitivity to input parameters. The calibrated model corresponds well with groundwater-level elevation observations, with a mean residual (observed minus simulated groundwater-level elevation) equal to −0.60 feet. Simulated groundwater base flow to streams was typically within 10 percent of base flow estimated by independent methods. Groundwater and surface-water interactions represented the largest water-budget components of the aquifer, with the second largest groundwater discharge component coming from pumping wells. Groundwater and surface-water interactions represent both the largest gain and loss terms in the water budget, because these interactions differ spatially, meaning in some areas of the model domain groundwater is being recharged by streams, whereas in other areas, groundwater is discharged to streams. Estimates of advective groundwater-flow paths indicate pumping wells may capture groundwater recharged from losing streams and groundwater that flows into the main stem of the alluvial aquifer from paleochannels.

Colorado

Results of Hydraulic Tests in Miocene Tuffaceous Rocks at the C-Hole Complex, 1995 to 1997, Yucca Mountain, Nye County, Nevada

Four hydraulic tests were conducted by the U.S. Geological Survey at the C-hole complex at Yucca Mountain, Nevada, between May 1995 and November 1997. These tests were conducted as part of ongoing investigations to determine the hydrologic and geologic suitability of Yucca Mountain as a potential site for permanent underground storage of high-level nuclear waste. The C-hole complex consists of three 900-meter-deep boreholes that are 30.4 to 76.6 meters apart. The C-holes are completed in fractured, variably welded tuffaceous rocks of Miocene age. Six hydrogeologic intervals occur within the saturated zone in these boreholes - the Calico Hills, Prow Pass, Upper Bullfrog, Lower Bullfrog, Upper Tram, and Lower Tram intervals. The Lower Bullfrog and Upper Tram intervals contributed about 90 percent of the flow during hydraulic tests. The four hydraulic tests conducted from 1995 to 1997 lasted 4 to 553 days. Discharge from the pumping well, UE-25 c #3, ranged from 8.49 to 22.5 liters per second in different tests. Two to seven observation wells, 30 to 3,526 meters from the pumping well, were used in different tests. Observation wells included UE-25 c #1, UE-25 c #2, UE-25 ONC-1, USW H-4, UE-25 WT #14, and UE-25 WT #3 in the tuffaceous rocks and UE-25 p #1 in Paleozoic carbonate rocks. In all hydraulic tests, drawdown in the pumping well was rapid and large (2.9-11 meters). Attributable mostly to frictional head loss and borehole-skin effects, this drawdown could not be used to analyze hydraulic properties. Drawdown and recovery in intervals of UE-25 c #1 and UE-25 c #2 and in other observation wells typically was less than 51 centimeters. These data were analyzed. Hydrogeologic intervals in the C-holes have layered heterogeneity related to faults and fracture zones. Transmissivity, hydraulic conductivity, and storativity generally increase downhole. Transmissivity ranges from 4 to 1,600 meters squared per day; hydraulic conductivity ranges from 0.1 to 50 meters per day; and storativity ranges from 0.00002 to 0.002. Transmissivity in the Miocene tuffaceous rocks decreases from 2,600 to 700 meters squared per day northwesterly across the 21-square-kilometer area affected by hydraulic tests at the C-hole complex. The average transmissivity of the tuffaceous rocks in this area, as determined from plots of drawdown in most or all observation wells as functions of time or distance from the pumping well, is 2,100 to 2,600 meters squared per day. Average storativity determined from these plot ranges is 0.0005 to 0.002. Hydraulic conductivity ranges from less than 2 to more than 10 meters per day; it is largest where prominent northerly trending faults are closely spaced or intersected by northwesterly trending faults. During hydraulic tests, the Miocene tuffaceous rocks functioned as a single aquifer. Drawdown occurred in all monitored intervals of the C-holes and other observation wells, regardless of the hydrogeologic interval being pumped. This hydraulic connection across geologic and lithostratigraphic contacts is believed to result from interconnected faults, fractures, and intervals with large matrix permeability. Samples of UE-25 c #3 water, analyzed from 1995 to 1997, seem to indicate that changes in the quality of the water pumped from that well are probably due solely to lateral variations in water quality within the tuffaceous rocks.

Water-Resources Investigations Report

Geodetic antenna calibration test in the Antarctic environment

TransAntarctic Mountain DEFormation (TAMDEF) Monitoring Network is the NSF-sponsored OSU and USGS project, aimed at measuring crustal motion in the Transantarctic Mountains of Victoria Land using GPS carrier phase measurements. Station monumentation, antenna mounts, antenna types, and data processing strategies were optimized to achieve mm-level estimates for the rates of motion. These data contributes also to regional Antarctic frame definition. Significant amount of data collected over several years allow the investigation of unique aspects of GPS geodesy in Antarctica, to determine how the error spectrum compares to the mid-latitude regions, and to identify the optimum measurement and data processing schemes for Antarctic conditions, in order to test the predicted rates of motion (mm-level w.r.t. time). The data collection for the TAMDEF project was initiated in 1996. The primary antenna used has been the Ashtech L1/L2 Dorne Margolin (D/M) choke ring. A few occupations involved the use of a Trimble D/M choke ring. The data were processed using the antenna calibration data available from the National Geodetic Survey (NGS). The recent developments in new antenna designs that are lighter in weight and lower in cost are being considered as a possible alternative to the bulkier and more expensive D/M choke ring design. In November 2003, in situ testing of three alternative models of L1/L2 antennas was conducted at a site located in the vicinity of McMurdo Station, Antarctica (S77.87, E166.56). The antenna models used in this test were: Ashtech D/M choke ring, Trimble D/M choke ring, Trimble Zephyr, and the NovAtel GPS-702. Two stations, spaced within 30 meters, were used in the test. Both had the characteristics similar to the stations of the TAMDEF network, i.e., the UNAVCO fixed-height, force-centered level mounts with a constant antenna offset were used, ensuring extreme stability of the antenna/ mount/pin set up. During each of the four 3-day test data collection sessions, a reference station was occupied continuously with the Ashtech D/M choke ring antenna, while the second station was occupied by the tested antennas, one 3-day session for each antenna type. The coordinate differences were produced using software optimized for the analysis of data collected over short baselines. Each solution incorporated the NGS antenna calibration data appropriate for each antenna model. Hourly and 24-hour solutions were analyzed for repeatability and compared to the standard baseline coordinate differences. No significant variation was observed when comparing the same type of antennas and when switching antennas at the test site using daily solutions. An mm-level scatter can be observed comparing different antennas over the 1-hour solutions; it is smaller for the horizontal components, as compared to the vertical direction. At this point, it can be concluded that the standard antenna calibration models from NGS used for each antenna involved in this test did not result in any significant variation in the daily results, but with some in the hourly results. Thus, based on this fact, the antenna types tested here could be used in the future TAMDEF campaigns, where 24-hour solutions are normally used for deformation monitoring. These results can serve as good guidance to any future use of GPS equipment in Antarctica.

Conference Paper

Summary of hydrologic testing of the Floridan aquifer system at Fort Stewart, coastal Georgia, 2009-2010

Two test wells were completed at Fort Stewart, coastal Georgia, to investigate the potential for using the Lower Floridan aquifer as a source of water to satisfy anticipated, increased water needs. The U.S. Geological Survey, in cooperation with the U.S. Department of the Army, completed hydrologic testing of the Floridan aquifer system at the study site, including flowmeter surveys, slug tests, and 24- and 72-hour aquifer tests by mid-March 2010. Analytical approaches and model simulation were applied to aquifer-test results to provide estimates of transmissivity and hydraulic conductivity of the multilayered Floridan aquifer system. Data from a 24-hour aquifer test of the Upper Floridan aquifer were evaluated by using the straight-line Cooper-Jacob analytical method. Data from a 72-hour aquifer test of the Lower Floridan aquifer were simulated by using axisymmetric model simulations. Results of aquifer testing indicated that the Upper Floridan aquifer has a transmissivity of 100,000 feet-squared per day, and the Lower Floridan aquifer has a transmissivity of 7,000 feet-squared per day. A specific storage for the Floridan aquifer system as a result of model calibration was 3E-06 ft–1. Additionally, during a 72-hour aquifer test of the Lower Floridan aquifer, a drawdown response was observed in two Upper Floridan aquifer wells, one of which was more than 1 mile away from the pumped well.

Georgia

Best practice guidelines for targeted environmental DNA-based proficiency testing in non-regulatory contexts

The effective use of environmental DNA (eDNA) tools is contingent on strict adherence to established and validated methods. Differences in eDNA methods and quality assurance protocols may contribute to variability in results. However, quality assurance measures such as proficiency testing can provide independent evaluation of laboratory performance against pre-established test criteria. With this commentary, we discuss how broad implementation of recurring proficiency testing in eDNA laboratories can build decision-maker confidence in eDNA results. It can also create a culture of continuous evaluation and improvement that minimizes error and meets performance requirements to inform the sustainable use or monitoring of natural resources. We provide an overview of proficiency testing across molecular disciplines, review the state of proficiency testing in eDNA applications, and draft a roadmap for the expanded application of proficiency testing informed by best practices for targeted eDNA detection. We suggest that best practice proficiency testing can be conducted by an independent, third-party sample provider. By demonstrating that laboratories are competent and capable of producing reliable results, implementation of proficiency testing best practices should foster confidence in eDNA measurements and its use in decision-making processes. Increased confidence in eDNA methods and a clear expectation of what is considered satisfactory performance are also likely to create more favorable conditions for investments in eDNA-based monitoring.

Environmental DNA

Interlaboratory comparison of three sediment bioaccumulation tests

Standard bioaccumulation tests are commonly conducted using Macoma nasuta (clam), and Alitta virens (polychaete) for marine tests, and Lumbriculus variegatus (an oligochaete) for freshwater tests. Because the interlaboratory variability associated with these tests is unknown, four experienced laboratories conducted standard 28-day bioaccumulation tests with the above species using sediments contaminated with polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs). Chemical analysis of tissue samples was performed by a single laboratory. The intralaboratory variance among replicates was relatively low for PCB tissue concentrations, with coefficients of variation (CVs) ranging from 9% to 28% for all laboratories and species, with the exception of one laboratory reporting higher variability for L . variegatus (CV = 51%). Intralaboratory variance for PCB tissue concentrations was higher than interlaboratory variance for A. virens and L . variegatus , and the magnitude of difference (MOD) for laboratory means ranged from 1.4 to 2.0 across species. Intralaboratory variability was also low for lipid content, and lipid normalization of PCB and PAH body residues generally had little impact on variability. In addition to variability across bioassay laboratories, analytical variability was evaluated by different laboratories measuring the concentration of PCBs and total lipids in a subsample of tissue homogenate of sediment-exposed test organisms. Variability associated with tissue analysis was higher than bioassay laboratory variability only in tests with L . variegatus . Statistical differences between samples may be observed due to the low intralaboratory variability; however, the biological significance of these differences may be limited because the MOD is low. Considering the MOD when comparing bioaccumulation across treatments accounts for uncertainty related to inherent variability of the test in the interpretation of statistically significant results.

Massachusetts

The use of slug tests to describe vertical variations in hydraulic conductivity

Multilevel slug tests provide one means of obtaining estimates of hydraulic conductivity on a scale of relevance for contaminant transport investigations. A numerical model is employed here to assess the potential of multilevel slug tests to provide information about vertical variations in hydraulic conductivity under conditions commonly faced in field settings. The results of the numerical simulations raise several important issues concerning the effectiveness of this technique. If the length of the test interval is of the order of the average layer thickness, considerable error may be introduced into the conductivity estimates owing to the effects of adjoining layers. The influence of adjoining layers is dependent on the aspect ratio (length of test interval/well radius) of the tesy interval and the flow properties of the individual layers. If a low-permeability skin is present at the well, the measured vertical variations will be much less than the actual variations, owing to the influence of the skin conductivity on the parameter estimates. A high-permeability skin can also produce apparent vertical variations that are much less than the actual, owing to water flowing vertically along the conductive skin. In cases where the test interval spans a number of layers, a slug test will yield an approximate thickness-weighted average of the hydraulic conductivities of the intersected layers. In most cases, packer circumvention should not be a major concern when packers of 0.75 m or longer are employed. Results of this study are substantiated by recently reported field tests that demonstrate the importance of well emplacement and development activities for obtaining meaningful estimates from a program of multilevel slug tests.

Journal of Hydrology

Multi-level slug tests in highly permeable formations: 1. Modification of the Springer-Gelhar (SG) model

A multi-level slug test model and a method for the evaluation of vertical profiles of hydraulic conductivity in highly permeable formations are developed. A double-packer system is employed to estimate local hydraulic conductivity. Depending on the formation and double-packer system parameters, the water level recovery in the tested well can exhibit a monotonic or oscillatory response. To discern information on aquifer properties from artifacts introduced by the measurement system, the theory of flow in an aquifer and a double-packer system is developed. The mathematical model incorporates features of the Springer and Gelhar (1991) model and reduces to the Bouwer and Rice (1976) model in a special case. The model involves equations of momentum and mass conservation for the double-packer system with quasi-steady well-aquifer interaction equations. The method is uniformly applicable for both monotonic and oscillatory well responses and can produce profiles of hydraulic conductivity for the tested well. The criterion is given to determine the type of well response for given slug test conditions.A multi-level slug test model and a method for the evaluation of vertical profiles of hydraulic conductivity in highly permeable formations are developed. A double-packer system is employed to estimate local hydraulic conductivity. Depending on the formation and double-packer system parameters, the water level recovery in the tested well can exhibit a monotonic or oscillatory response. To discern information on aquifer properties from artifacts introduced by the measurement system, the theory of flow in an aquifer and a double-packer system is developed. The mathematical model incorporates features of the Springer and Gelhar (1991) model and reduces to the Bouwer and Rice (1976) model in a special case. The model involves equations of momentum and mass conservation for the double-packer system with quasi-steady well-aquifer interaction equations. The method is uniformly applicable for both monotonic and oscillatory well responses and can produce profiles of hydraulic conductivity for the tested well. The criterion is given to determine the type of well response for given slug test conditions.

Journal of Hydrology

Biodegradation of the surfactant linear alkylbenzenesulfonate in sewage-contaminated groundwater: A comparison of column experiments and field tracer tests

Transport and biodegradation of linear alkylbenzenesulfonate (LAS) in sewage-contaminated groundwater were investigated for a range of dissolved oxygen concentrations. Both laboratory column and an 80-day continuous injection tracer test field experiments were conducted. The rates of LAS biodegradation increased with increasing dissolved oxygen concentrations and indicated the preferential biodegradation of the longer alkyl chain LAS homologues (i.e., C12 and C13) and external isomers (i.e., 2-and 3- phenyl). However, for similar dissolved oxygen concentrations, mass removal rates for LAS generally were 2-3 times greater in laboratory column experiments than in the field tracer test. Under low oxygen conditions (<1 mg/L) only a fraction of the LAS mixture biodegraded in both laboratory and field experiments. Biodegradation rate constants for the continuous injection field test (0.002-0.08 day-1) were comparable to those estimated for a 3-h injection (pulsed) tracer test conducted under similar biogeochemical conditions, indicating that increasing the exposure time of aquifer sediments to LAS did not increase biodegradation rates.Transport and biodegradation of linear alkylbenzenesulfonate (LAS) in sewage-contaminated groundwater were investigated for a range of dissolved oxygen concentrations. Both laboratory column and an 80-day continuous injection tracer test field experiments were conducted. The rates of LAS biodegradation increased with increasing dissolved oxygen concentrations and indicated the preferential biodegradation of the longer alkyl chain LAS homologues (i.e., C12 and C13) and external isomers (i.e., 2- and 3-phenyl). However, for similar dissolved oxygen concentrations, mass removal rates for LAS generally were 2-3 times greater in laboratory column experiments than in the field tracer test. Under low oxygen conditions (<1 mg/L) only a fraction of the LAS mixture biodegraded in both laboratory and field experiments. Biodegradation rate constants for the continuous injection field test (0.002-0.08 day-1) were comparable to those estimated for a 3-h injection (pulsed) tracer test conducted under similar biogeochemical conditions, indicating that increasing the exposure time of aquifer sediments to LAS did not increase biodegradation rates.

Environmental Science & Technology

Comparative evaluation of molecular diagnostic tests for Nucleospora salmonis and prevalence in migrating juvenile salmonids from the Snake River, USA

Nucleospora salmonis is an intranuclear microsporidian that primarily infects lymphoblast cells and contributes to chronic lymphoblastosis and a leukemia-like condition in a range of salmonid species. The primary goal of this study was to evaluate the prevalence of N. salmonis in out-migrating juvenile hatchery and wild Chinook salmon Oncorhynchus tshawytscha and steelhead O. mykiss from the Snake River in the U.S. Pacific Northwest. To achieve this goal, we first addressed the following concerns about current molecular diagnostic tests for N. salmonis : (1) nonspecific amplification patterns by the published nested polymerase chain reaction (nPCR) test, (2) incomplete validation of the published quantitative PCR (qPCR) test, and (3) whether N. salmonis can be detected reliably from nonlethal samples. Here, we present an optimized nPCR protocol that eliminates nonspecific amplification. During validation of the published qPCR test, our laboratory developed a second qPCR test that targeted a different gene sequence and used different probe chemistry for comparison purposes. We simultaneously evaluated the two different qPCR tests for N. salmonis and found that both assays were highly specific, sensitive, and repeatable. The nPCR and qPCR tests had good overall concordance when DNA samples derived from both apparently healthy and clinically diseased hatchery rainbow trout were tested. Finally, we demonstrated that gill snips were a suitable tissue for nonlethal detection of N. salmonis DNA in juvenile salmonids. Monitoring of juvenile salmonid fish in the Snake River over a 3-year period revealed low prevalence of N. salmonis in hatchery and wild Chinook salmon and wild steelhead but significantly higher prevalence in hatchery-derived steelhead. Routine monitoring of N. salmonis is not performed for all hatchery steelhead populations. At present, the possible contribution of this pathogen to delayed mortality of steelhead has not been determined.

Journal of Aquatic Animal Health

AIRSLUG: A fortran program for the computation of type curves to estimate transmissivity and storativity from prematurely terminated air-pressurized slug tests

The Fortran code AIRSLUG can be used to generate the type curves needed to analyze the recovery data from prematurely terminated air-pressurized slug tests. These type curves, when used with a graphical software package, enable the engineer or scientist to analyze field tests to estimate transmissivity and storativity. Prematurely terminating the slug test can significantly reduce the overall time needed to conduct the test, especially at low-permeability sites, thus saving time and money.The Fortran code AIRSLUG can be used to generate the type curves needed to analyze the recovery data from prematurely terminated air-pressurized slug tests. These type curves, when used with a graphical software package, enable the engineer or scientist to analyze field tests to estimate transmissivity and storativity. Prematurely terminating the slug test can significantly reduce the overall time needed to conduct the test, especially at low-permeability sites, thus saving time and money.

Ground Water

Analytical validation of a real-time reverse transcription polymerase chain reaction test for Pan-American lineage H7 subtype Avian influenza viruses

A real-time reverse transcription polymerase chain reaction test for the identification of the H7 subtype in North American Avian influenza viruses (AIVs) was first reported in 2002; however, recent AIV surveillance efforts in wild birds and H7 outbreaks in poultry demonstrated that the 2002 test did not detect all H7 AIVs present in North and South America. Therefore, a new test, the 2008 Pan-American H7 test, was developed by using recently available H7 nucleotide sequences. The analytical specificity of the new assay was characterized with an RNA panel composed of 19 H7 viruses from around the world and RNA from all hemagglutinin subtypes except H16. Specificity for North and South American lineage H7 viruses was observed. Assay limits of detection were determined to be between 103 and 104 gene copies per reaction with in vitro transcribed RNA, and 100.0 and 10 0.8 50% egg infectious doses per reaction. The 2008 Pan-American H7 test also was shown to perform similarly to the 2002 test with specimens from chickens experimentally exposed to A/Chicken/BritishColumbia/314514-2/04 H7N3 highly pathogenic AIV. Furthermore, the 2008 test was able to detect 100% (n = 27) of the H7 AIV isolates recovered from North American wild birds in a 2006-2007 sample set (none of which were detected by the 2002 H7 test).

Journal of Veterinary Diagnostic Investigation

Goodness-of-fit tests for open capture-recapture models

General goodness-of-fit tests for the Jolly-Seber model are proposed. These tests are based on conditional arguments using minimal sufficient statistics. The tests are shown to be of simple hypergeometric form so that a series of independent contingency table chi-square tests can be performed. The relationship of these tests to other proposed tests is discussed. This is followed by a simulation study of the power of the tests to detect departures from the assumptions of the Jolly-Seber model. Some meadow vole capture-recapture data are used to illustrate the testing procedure which has been implemented in a computer program available from the authors.

Biometrics

Core logs from five test holes near Kramer, California

In 1957, five test holes were drilled near Kramer, Calif., in the western Mojave Desert, for the U.S. Geological Survey. The drill sites are in topographic basins where gravimetric and geologic surveys indicated the presence, beneath alluvium, of a thick section of Quaternary and Tertiary sedimentary and volcanic rocks. Two holes, which were deeper tests at sites drilled in 1954, cored only silts, sands, and gravels: Four Corners test hole. 1 was drilled in sec. 20, T. 10 N., R. 6 W., to a depth of 3,500 feet. Four Corners test hole 2, in sec. 5, T. 10 N., R. 8 W., was drilled to 2,328 feet. Three holes which were drilled at new sites north of Kramer Junction, the intersection of U.S. Highways 395 and 466 and locally known as Four Corners, penetrated colemanite-bearing sediments. The location and total depth of these holes, are as follows: Four Corners test hole 3, sec. 18, T. 11 N., R. 6 W., depth 2,568. feet; Four Corners test hole 4, near the north edge of sec. 30, T. 11 N., R. 6 W., 1 depth 3,500 feet; Four Corners test hole 5, near the south edge of sec. 30, depth 1,604 feet. The sections of rocks penetrated in these 3 holes are similar. In each, the colemanite is in fine-grained sediments; these lie below sands and gravels, which are about 600 to 800 feet thick, and are underlain by sandstones and conglomerates. Colemanite is most abundant in the cores from Four Corners test hole 5, particularly in the 76 feet of core recovered between depths of 1,051 and 1,131 feet. Chemical analysis indicates that in this section of core the average content of BaOs is above 14 percent. In addition to colemanite, the cores contain sulfides of arsenic, an unusual iron sulfide, and zeolites. This mineral ,content of the colemanite-bearing sediments north of Kramer Junction (Four Corners), together with the general lithology of the lake beds and the occurrence of the sediments as a tilted section of beds below sands and gravels, supports correlation with the upper or marginal parts of the borate-bearing sediments at the Kramer borate mining district, which have similar features. There is, however, no evidence that any beds are exactly equivalent in age.

Bulletin