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Critically assessing the utility of portable lead analyzers for wildlife conservation

Lead (Pb) exposure in wildlife is a widespread management and conservation concern. Quantitative determination of Pb concentrations in wildlife tissues is the foundation for estimating exposure and risk. Development of low‐cost, portable instruments has improved access and cost‐effectiveness of determining Pb concentrations in blood samples, while also facilitating the ability for wildlife researchers to conduct near real‐time Pb testing. However, these instruments, which use anodic stripping voltammetry (ASV) methodology, may produce an analytical bias in wildlife‐blood Pb concentrations. Additionally, their simplicity invites use without appropriate quality‐assurance–quality‐control measures. Together, these factors can reduce data quality and hamper the ability to evaluate it, raising concerns about use of these instruments to inform important conservation issues. We document the extent to which this bias is addressed in the wildlife toxicology literature, develop quantitative approaches for correcting the bias, and provide recommendations to ensure robust data quality when using these instruments. Of the 25 studies we reviewed that referenced ASV use for determining Pb exposure in wildlife, only 32% acknowledged the existence of bias from the instrument. Importantly, another 20% of the studies actually reported ASV and spectroscopic‐based results together without acknowledging their lack of equivalence. Using a multispecies data set of avian blood Pb concentrations, we found that ASV‐based estimates of paired blood Pb concentrations were 30–38% lower than those from standard spectrometric‐based methods. We provide regression equations based on this analysis of 453 blood samples to allow users of ASV instruments to adjust Pb concentrations to spectrometric‐equivalent values, and propose a series of guidelines to follow when using these instruments to improve data validity.

Wildlife Society Bulletin

Seismic tomography 2023

Seismic tomography is the most abundant source of information about the internal structure of the Earth at scales ranging from a few meters to thousands of kilometers. It constrains the properties of active volcanoes, earthquake fault zones, deep reservoirs and storage sites, glaciers and ice sheets, or the entire globe. It contributes to outstanding societal problems related to natural hazards, resource exploration, underground storage, and many more. The recent advances in seismic tomography are being translated to nondestructive testing, medical ultrasound, and helioseismology. Nearly 50 yr after its first successful applications, this article offers a snapshot of modern seismic tomography. Focused on major challenges and particularly promising research directions, it is intended to guide both Earth science professionals and early‐career scientists. The individual contributions by the coauthors provide diverse perspectives on topics that may at first seem disconnected but are closely tied together by a few coherent threads: multiparameter inversion for properties related to dynamic processes, data quality, and geographic coverage, uncertainty quantification that is useful for geologic interpretation, new formulations of tomographic inverse problems that address concrete geologic questions more directly, and the presentation and quantitative comparison of tomographic models. It remains to be seen which of these problems will be considered solved, solved to some extent, or practically unsolvable over the next decade.

Bulletin of the Seismological Society of America

Estimates of k0 and effects on ground motions in the San Francisco Bay area

Ground‐motion studies are a key component of seismic hazard analyses and often rely on information of the source, path, and site. Extensive research has been done on each of these parameters; however, site‐specific studies are of particular interest to seismic hazard studies, especially in the field of earthquake engineering, as near‐site conditions can have a significant impact on the resulting ground motion at a site. There has been much focus on the constraint of site parameters and their application to seismic hazard studies, especially in the development of ground‐motion models (GMMs). Kappa is an observational parameter describing the high‐frequency attenuation of spectra, and its site contribution ( ⁠ κ 0 "> κ 0 ) has shown to be a good predictor of high‐frequency ground motions; however, measurements are often limited. In this study, we develop a κ 0 "> κ 0 dataset for the San Francisco Bay area (SFBA) by estimating κ 0 "> κ 0 for 228 stations, and we produce a continuous regional map of κ 0 "> κ 0 ⁠ . We find κ 0 "> κ 0 to range between 0.003 and 0.072 s, with larger values concentrating on the east, north, and south sides of the bay, and lower values concentrating on the west side. We also evaluate the robustness of κ 0 "> κ 0 as a site parameter and find it to correlate with peak ground acceleration. These estimates of κ 0 "> κ 0 can add predictive power to GMMs, thus increasing the accuracy of predicted ground motion and improving the robustness of ground‐motion studies in the SFBA.

California

Comparison of data handling techniques for modeling bat acoustic activity

With the proliferation of acoustic sampling to investigate bat distribution and ecology, researchers have implemented a myriad of statistical modeling approaches to interpret findings. Bats are taxa of high conservation concern; therefore, ensuring the accuracy of species-level habitat association models is critical for informing management. We sought to determine prediction differences among statistical approaches to modeling counts of acoustic detections, using generalized linear mixed models with 8 acoustic data-handling techniques. We applied each approach or combination of approaches to a rare species, the northern long-eared bat ( Myotis septentrionalis ), and a common species, the eastern red bat ( Lasiurus borealis ), from summer survey results on a landscape in south-central Pennsylvania, USA, 2024. We evaluated the accuracy of habitat association models of bat acoustic activity at the species level using cross-validation and compared resulting predictions of models using spatial correlations. We determined that filtering data by automated identification software (Kaleidoscope Pro), the maximum likelihood estimate (MLE) P -value thresholds reduced relative mean absolute error (rMAE) in cross-validation of northern long-eared bat models. Using the MLE-retained data produced the most accurate predictions over using raw data or the overly conservative match ratio data. However, for the eastern red bat, the results from the most conservative approach of only retaining data with at least a 90% match ratio from software development training sets had the lowest rMAE. We have provided evidence that the current standard of filtering data by nightly MLE can result in more accurate and informative habitat-use acoustic activity models for rare bat species.

Pennsylvania

Advanced quantitative precipitation information: Improving monitoring and forecasts of precipitation, streamflow, and coastal flooding in the San Francisco Bay area

Advanced Quantitative Precipitation Information (AQPI) is a synergistic project that combines observations and models to improve monitoring and forecasts of precipitation, streamflow, and coastal flooding in the San Francisco Bay Area. As an experimental system, AQPI leverages more than a decade of research, innovation, and implementation of a statewide, state-of-the-art network of observations, and development of the next generation of weather and coastal forecast models. AQPI was developed as a prototype in response to requests from the water management community for improved information on precipitation, riverine, and coastal conditions to inform their decision-making processes. Observation of precipitation in the complex Bay Area landscape of California’s coastal mountain ranges is known to be a challenging problem. But, with new advanced radar network techniques, AQPI is helping fill an important observational gap for this highly populated and vulnerable metropolitan area. The prototype AQPI system consists of improved weather radar data for precipitation estimation; additional surface measurements of precipitation, streamflow, and soil moisture; and a suite of integrated forecast modeling systems to improve situational awareness about current and future water conditions from sky to sea. Together these tools will help improve emergency preparedness and public response to prevent loss of life and destruction of property during extreme storms accompanied by heavy precipitation and high coastal water levels—especially high-moisture laden atmospheric rivers. The Bay Area AQPI system could potentially be replicated in other urban regions in California, the United States, and worldwide.

California

Evaluating the risk of SARS-CoV-2 transmission to bats in the context of wildlife research, rehabilitation, and control

Preventing wildlife disease outbreaks is a priority for natural resource agencies, and management decisions can be urgent, especially in epidemic circumstances. With the emergence of SARS-CoV-2, wildlife agencies were concerned whether the activities they authorize might increase the risk of viral transmission from humans to North American bats, but had a limited amount of time in which to make decisions. We describe how decision analysis provides a powerful framework to analyze and reanalyze complex natural resource management problems as knowledge evolves. Coupled with expert judgment and avenues for the rapid release of information, risk assessment can provide timely scientific information for evolving decisions. In April 2020, the first rapid risk assessment was conducted to evaluate the risk of transmission of SARS-CoV-2 from humans to North American bats. Based on the best available information and relying heavily on expert judgment, the risk assessment found a small possibility of transmission during summer work activities. Following that assessment, additional knowledge and data emerged, such as bat viral challenge studies, that further elucidated the risks of human-to-bat transmission and culminated in a second risk assessment in the fall of 2020. We updated the first SARS-CoV-2 risk assessment with new management alternatives and new estimates of little brown bat ( Myotis lucifugus ) susceptibility, using findings from the fall 2020 assessment and other empirical studies. We found that new knowledge led to an 88% decrease in the median number of bats estimated to be infected per 1,000 encountered when compared to earlier results. The use of facemasks during, or a negative COVID-19 test or vaccination prior to, bat encounters further reduced those risks. Using a combination of decision analysis, expert judgment, rapid risk assessment, and efficient modes of information distribution, we provided timely science-based support to decision makers for summer bat work in North America.

Wildlife Society Bulletin

Topographic Slope as a Proxy for Seismic Site-Conditions (VS30) and Amplification Around the Globe

Executive Summary It is well-known that large global earthquakes can have a dramatic effect on local communities and the built environment. Moreover, ground motions amplified by surficial materials can exacerbate the situation, often making the difference between minor and major damage. For a real-time earthquake impact alert system, such as Prompt Assessment of Global Earthquakes for Response (PAGER) (Wald and others, 2006), we seek to rapidly evaluate potential ground shaking in the source region and subsequently provide an estimate of the population exposure to potentially fatal levels of ground shaking in any region of the world. The contribution of surficial geology (particularly soft sediments) to the amplification of ground shaking is an important component in predicting the levels of ground motion observed at any site. Unfortunately, the availability of information regarding seismic siteconditions is only available at a few sites around the globe. Herein, we describe a methodology for deriving maps of seismic site-conditions anywhere in the world using topographic slope as a proxy. Average shear-velocity down to 30 m (or VS30) measurements are correlated against topographic slope to develop two sets of coefficients for predicting VS30: one for active tectonic regions that possess dynamic topographic relief, and one for stable continental regions where changes in topography are more subdued. These coefficients have been applied to the continental United States, in addition to other regions around the world. They are subsequently compared to existing site-condition maps based on geology and observed VS30 measurements, where available. The application of the topographic slope method in regions with abundant VS30 measurements (for example California, Memphis, and Taiwan) indicates that this method provides site condition-maps of similar quality, or in some cases, maps superior to those developed from more traditional techniques. Having a first-order assessment of seismic site-conditions anywhere in the world provides a valuable tool to rapidly estimate ground motions following any global earthquake, the primary motivation for this research. These VS30 maps will enable us to better quantify possible ground shaking and rapidly deliver these predictions to emergency managers and responders. In addition, the VS30 maps for the globe will also have practical applications for numerous related probabilistic- and scenario-based studies. To date, several researchers have requested maps or have used the approach outlined herein for their own applications (for example Cagnan and Kariptas, written commun., 2007; Harmandar and others, 2007). Given that we anticipate a significant demand for these products, we have developed an internet delivery service so that users can download maps and grids of seismic site-conditions for specified regions. To some extent, these grids can also be customized by the user if they disagree with the predefined correlations derived using the methodologies described within this report. Finally, this report represents a more comprehensive account of this technique and provides a more fully illustrated global description of results than that given in Wald and Allen (2007), which has been accepted for publication in the Bulletin of the Seismological Society of America.

Open-File Report

Geologic characteristics of sediment- and volcanic-hosted disseminated gold deposits - Search for an occurrence model

The current expansion of resource information, particularly on "disseminated" gold, and the improved technologies now available for resource investigations should place us in an enhanced position for developing a better predictive methodology for meeting one of the important responsibilities of the U.S. Geological Survey-to examine and assess the mineral resources of the geologic terranes composing the public (and privately owned) lands of the United States. The first step is systematic organization of these data. Geologic-occurrence models are an effective systematic method by which to organize large amounts of resource information into a logical sequence facilitating its use more effectively in meeting several industry and Survey objectives, which include the exploration for resources and the assessment of resource potential for land-use decisions. Such models also provide a scientific basis for metallogenesis research, which considers the observable features or attributes of ore occurrence and their "fit" into the Earth's resource puzzle. The use of models in making resource assessments/appraisals was addressed by Shawe (1981), who reported the results of a workshop on methods for resource appraisal of Wilderness and Conterminous United States Mineral Appraisal Program (CUSMAP; 1:250,000-scale quadrangles) areas. The Survey's main objective in the 1982 workshop was to evaluate the status of knowledge about disseminated or very fine grained gold deposits and, if possible, to develop an occurrence model(s). This report on the workshop proceedings has three main objectives: (1) Education through the publication of a summary review and presentation of new thinking and observations about the scientific bases for those geologic processes and environments that foster disseminated gold-ore formation; (2) systematic organization of available geologic, geochemical, and geophysical information for a range of typical disseminated gold deposits (including recognition of gaps in those data); and (3) assessment of current understanding (as presented in objective 2) toward formulating an empirical ore-occurrence model for this type of deposit. As such, this volume represents a preliminary first step at classification and provides a source of pertinent background information. Readers of this volume will soon discover, however, that full agreement has not yet been achieved in the interpretation of some of the geologic evidence. The resulting variations in tentative occurrence models for these controversial deposits ultimately will be resolved by filling the gaps in information that have already been identified. Thus, this volume does not report a U.S. Geological Survey consensus; the conclusions expressed in each chapter represent the particular interpretations of the various workshop participants.

Arizona, California, Idaho, Nevada, Utah

Declines of greater and lesser scaup populations: issues, hypotheses, and research needs

The population estimate for greater (Aythya marila) and lesser (Aythya affinis) scaup (combined) has declined dramatically since the early 1980s to record lows in 1998. The 1998 estimate of 3.47 million scaup is far below the goal of 6.3 million set in the North American Waterfowl Management Plan (NAWMP), causing concern among biologists and hunters. We summarize issues of concern, hypotheses for factors contributing to the population decline, and research and management needs recommended by participants of the Scaup Workshop, held in September 1999. We believe that contaminants, lower female survival, and reduced recruitment due to changes in food resources or breeding-ground habitats are primary factors contributing to the decline. These factors are not mutually exclusive but likely interact across seasons. Workshop participants identified seven action items. We need to further delineate where declines in breeding populations have occurred, with a primary focus on the western Canadian boreal forest, where declines appear to be most pronounced. Productivity in various areas and habitats throughout the breeding range needs to be assessed by conducting retrospective analyses of existing data and by intensive field studies at broad and local scales. Annual and seasonal survival rates need to be determined in order to assess the role of harvest or natural mortality. Effects of contaminants on reproduction, female body condition, and behavior must be investigated. Use, distribution, and role of food resources relative to body condition and reproduction need to be examined to better understand seasonal dynamics of nutrient reserves and the role in reproductive success. Affiliations among breeding, migration, and wintering areas must be assessed in order to understand differential exposure to harvest or contaminants, and differential reproductive success and recruitment. Biologists and agencies need to gather and improve information needed to manage greater and lesser scaup separately; this includes monitoring the breeding populations of each species separately, closer examination of existing data to improve surveys and data collection, and re-evaluation of the NAWMP population goal. These complex issues will require extensive cooperation and communication among many agencies and organizations in North America.

Wildlife Society Bulletin

Correlation of the outcropping cretaceous formations of the Atlantic and Gulf Coastal Plain and trans-Pecos Texas

This is Number 9 of a series of correlation charts prepared by the Committee on Stratigraphy of the National Research Council, which will cover the post-Proterozoic sedimentary formations of North America. For general information about the project the reader is referred to the general introduction preceding this paper. The senior author, Lloyd W. Stephenson, is responsible for that part of the chart treating of the Upper Cretaceous series in the Atlantic and Gulf Coastal Plain (exceptions noted below) and the Lower Cretaceous series of the North Atlantic Coastal Plain; with respect to the Upper Cretaceous his presentation is based largely on his personal knowledge of the paleontology and stratigraphy of the area, gained from field and laboratory studies ranging from reconnaissance to detailed; all published information has been considered, and much of it utilized, but it is not practicable to include a complete bibliography. References are given to papers presenting views differing from those of the author, and to recent papers not yet listed in bibliographies. A complete bibliography of Texas geology through 1932 is given by Sellards (1933, p. 819-965). Watson H. Monroe has collaborated in the preparation of that part of the chart showing therelations of the Upper Cretaceous units in Alabama and Mississippi. The classification shown in the Upper Cretaceous part of the Delaware column is based on a recently published paper by Charles W. Carter (1937, p. 237-281). Philip B. King is responsible for the remainder of the chart covering the Lower Cretaceous (Comanche series) of central Texas (largely compiled from published sources) and all of the Cretaceous in trans-Pecos Texas (from personal knowledge and published sources). In March 1941 this part of the chart was checked by R. W. Imlay, and changes were made by King at his suggestion. References to the more important literature are given. In a correlation chart of this kind, in which age equivalencies are expressed by horizontal lining, it is obviously impossible to indicate relative thicknesses by vertical spacing. Hiatuses are indicated by vertical lining. The red-line overprint is intended to indicate certain important lithologic and faunal boundaries, and to assist the eye in recognizing the age relationships of the geologic units

Geological Society of America Bulletin

FLUXNET-CH4 synthesis activity: Objectives, observations, and future directions

This paper describes the formation of, and initial results for, a new FLUXNET coordination network for ecosystem-scale methane (CH 4 ) measurements at 60 sites globally, organized by the Global Carbon Project in partnership with other initiatives and regional flux tower networks. The objectives of the effort are presented along with an overview of the coverage of eddy covariance (EC) CH 4 flux measurements globally, initial results comparing CH 4 fluxes across the sites, and future research directions and needs. Annual estimates of net CH 4 fluxes across sites ranged from −0.2 ± 0.02 g C m –2 yr –1 for an upland forest site to 114.9 ± 13.4 g C m –2 yr –1 for an estuarine freshwater marsh, with fluxes exceeding 40 g C m –2 yr –1 at multiple sites. Average annual soil and air temperatures were found to be the strongest predictor of annual CH 4 flux across wetland sites globally. Water table position was positively correlated with annual CH 4 emissions, although only for wetland sites that were not consistently inundated throughout the year. The ratio of annual CH 4 fluxes to ecosystem respiration increased significantly with mean site temperature. Uncertainties in annual CH 4 estimates due to gap-filling and random errors were on average ±1.6 g C m –2 yr –1 at 95% confidence, with the relative error decreasing exponentially with increasing flux magnitude across sites. Through the analysis and synthesis of a growing EC CH 4 flux database, the controls on ecosystem CH 4 fluxes can be better understood, used to inform and validate Earth system models, and reconcile differences between land surface model- and atmospheric-based estimates of CH 4 emissions.

Bulletin of the American Meteorological Society

Developing a photography-based harvest survey to estimate age and subspecies composition of midcontinent sandhill cranes

Midcontinent sandhill cranes ( Antigone canadensis ) are managed as a single population, but hunting regulations are structured so harvest is targeted towards the more numerous lesser sandhill cranes ( A. c. canadensis ). However, research indicates that greater sandhill cranes ( A. c. tabida ) have been disproportionally exposed to harvest at a rate exceeding their proportion within the midcontinent population. In addition, harvest has increased 22% per year in the U.S. Central Flyway states. The midcontinent population appears to be growing in recent years, but variability in annual abundance estimates has increased substantially. With limited resources and harvest management uncertainty increasing, we developed methods for a citizen science, photography-based harvest survey to estimate age and subspecies composition of harvested midcontinent sandhill cranes. To develop survey methods, we collected physical parts from 284 sandhill cranes in North Dakota in 2019 and 2020. We manually measured the culmen and tarsus using calipers, and digitally measured these parts using photographs and computer software. All digitally derived measurements were 2.5% to 5.9% larger than manual measurements; therefore, we developed linear models that adjusted digital measurements, facilitating subspecies prediction using an existing morphometric-based technique. In 2021, we requested an equal number of hunters to participate using 2 data collection methods to test if hunters could reliably take photographs suitable for digital measurement. Collection method 1 involved photographing the head and leg simultaneously, and Collection method 2 involved photographing the head only. Hunters submitted a total of 239 photographs. Only 80 of these photographs were submitted using Collection method 1, and 72% were suitable for digital measurement. Conversely, hunters submitted twice as many photographs using Collection method 2, and 88% of these photographs were deemed suitable. Although obtaining the tarsus measurement slightly improved subspecies predictability, Collection method 2 increased participation and usable data. We believe our results could be used to develop an operational survey of age and subspecies composition of midcontinent sandhill cranes, wherein a sample of crane hunters throughout the midcontinent population range would be asked to electronically submit a photograph of the head of each bird they harvest. A time series of age and subspecies composition of this population would provide managers with valuable information and improve harvest management at minimal additional cost and burden, compared to a traditional parts collection survey administered by mail.

Wildlife Society Bulletin

Late Neogene–Quaternary tephrochronology, stratigraphy, and paleoclimate of Death Valley, California, USA

Sedimentary deposits in midlatitude continental basins often preserve a paleoclimate record complementary to marine-based records. However, deriving that paleoclimate record depends on having well-exposed deposits and establishing a sufficiently robust geochronology. After decades of research, we have been able to correlate 77 tephra beds exposed in multiple stratigraphic sections in the Death Valley area, California, United States. These correlations identify 25 different tephra beds that erupted from at least five different volcanic centers from older than 3.58 Ma to ca. 32 ka. We have informally named and determined the ages for seven previously unrecognized beds: ca. 3.54 Ma tuff of Curry canyon, ca. 3.45 Ma tuff of Furnace Creek, ca. 3.1 Ma tuff of Kit Fox Hills, ca. 3.1 Ma tuff of Mesquite Flat, ca. 3.15 Ma tuff of Texas Spring, 3.117 ± 0.011 Ma tuff of Echo Canyon, and the ca. 1.3 Ma Amargosa ash bed. Several of these tephra beds are found as far northeast as central Utah and could be important marker beds in western North America. Our tephrochronologic data, combined with magnetic polarity data and 40 Ar/ 39 Ar age determinations, redefine Neogene sedimentary deposits exposed across 175 km 2 of the Death Valley area. The alluvial/lacustrine Furnace Creek Formation is a time-transgressive sedimentary sequence ranging from ca. 6.0 to 2.5 Ma in age. The ca. 2.5–1.7 Ma Funeral Formation is typically exposed as a proximal alluvial-fan facies overlying the Furnace Creek Formation. We have correlated deposits in the Kit Fox Hills, Salt Creek, Nova Basin, and southern Death Valley with the informally named ca. 1.3–0.5 Ma Mormon Point formation. In addition, our correlation of the late Pleistocene Wilson Creek ash bed 15 in the Lake Rogers deposits represents the first unambiguous sequences deposited during the Last Glacial Maximum (marine isotope stage [MIS] 2) in Death Valley. Based on this new stratigraphic framework, we show that the Pliocene and Pleistocene climate in Death Valley is consistent with the well-established marine tropical/subtropical record. Pluvial lakes in Death Valley and Searles Valley began to form ca. 3.5–3.4 Ma in the late Pliocene during MIS MG5. Initiation of lakes in these two hydrologically separated valleys at the same time at the beginning of a cooling trend in the marine climate record suggests a link to a cooler, wetter (glacial) regional climate in North America. The Death Valley lake persisted until ca. 3.30 Ma, at the peak of the M2 glaciation, after which there is no evidence of Pliocene lacustrine deposition, even at the peak of the Northern Hemisphere Glaciation (ca. 2.75 Ma). If pluvial lakes in the Pliocene are an indirect record of glacial climate conditions, as they are for the Pleistocene, then a glacial climate was present in western North America for ∼200,000 yr during the Pliocene, encompassing MIS MG5–M2. Pleistocene pluvial lakes in Death Valley that formed ca. 1.98–1.78 Ma, 1.3–1.0 Ma, and ca. 0.6 Ma (MIS 16) are consistent with other regional climate records that indicate a regional glacial climate; however, Death Valley was relatively dry at ca. 0.77 Ma (MIS 19), when large lakes existed in other basins. The limited extent of the MIS 2 marsh/shallow lake in the Lake Rogers basin of northern Death Valley reflects the well-known regional glacial climate at that time; however, Death Valley received relatively lower inflow and rainfall in comparison.

Geological Society of America Bulletin

A life history study of the yellow throat

Investigations concerning the life history of the Yellow-throat were made in southern Michigan during the spring and summer of 1938. Supplementary information was also obtained at Arlington, Virginia, in 1940 and at the Patuxent Research Refuge, Maryland, in 1947.....Resident males established territories almost immediately upon arrival in spring. In southern Michigan some resident males arrived at least as soon as, if not before, transient males. Most females appeared on their nesting ground about a week later. Adults were engaged in nesting activities from the time of their arrival in spring until the advent of the post-nuptial molt in late summer.....Typical Yellow-throat habitat consists of a mixture of a dense herbaceous vegetation and small woody plants in damp or wet situations. At Ann Arbor, the Yellow-throat was a common breeding species in its restricted suitable habitat. The population density in one area of suitable habitat was about 69 territorial males per 100 acres. Of 11 territorial males that were intensively studied, one was polygamous (with two mates), nine were monogamous, and one was probably monogamous (with at least one mate).....The song of the individual Yellow-throat was heard throughout the breeding season except for the courtship period. Two major types of song were the common song given while perched, and an occasional, more elaborate, flight song. Most males sing in spurts, singing at fairly regular intervals for a considerable period and then abruptly ceasing for another period. The vocabulary of both sexes included several types of call notes that appeared either to have special functions or to represent outward expressions of distinct emotional states of the bird.....Resident males were antagonistic toward each other throughout the breeding season. Most remained on well-established territories during this period. Territories of 10 monogamous males ranged in size from .8 to 1.8 acres but the territory of one polygamous male occupied. 3.4 acres. The behavior of males during inter-territorial encounters was similar in some respects to their behavior when courting females.....While courting females, the males are very attentive and seldom sing for about one week. During the courtship period the female locates the nesting site and builds the nest without assistance from the male.....Nests, constructed of dried plant materials, were situated on or near the ground and were supported on all sides by stems of herbaceous plants or limbs of shrubs. Many nests were composed of three layers with the coarser materials being used in the outer layer.....The full clutch of eggs in 12 nests ranged from 4 to 6 (average, 4.6). Early clutches seem to be larger than later ones. After the first egg is laid, one is laid on each succeeding day until the clutch is complete. Incubation period is about 12 days. Incubation is only by the female. Records of daytime incubation schedules of three females about half way through incubation indicate that the periods spent on and off the nests average about 61 and 16 minutes, respectively.....Young Yellow-throats usually remain in the nest for eight or nine days. During this period they grow and develop rapidly. Their weight quintuples in six days. Both sexes are active in feeding the young and in removing excreta from the nest. Records of feeding at three nests showed a range of one feeding per 5 1/2 minutes to one feeding per 22 minutes, the rate increasing with age of young. Adults care for the young for at least two weeks after the young leave the nest.....Ten of 12 females that were intensively studied were successful in raising young beyond the nestling stage. Only one of these raised two broods, although three females built at least three nests each. In 19 nests, 11 (58 per cent) produced nestlings and 7 (37 per cent) produced fledglings. In total, the 19 nests produced an average of one fledgling Yellow-throat per nest. Of 22 nests that were found near Ann Arbor, 10 (45

The Wilson Bulletin