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At least 163 records · Page 9Linked to original sources
USGS2016

Archive of Digital Chirp Subbottom Profile Data Collected During USGS Cruise 14BIM05 Offshore of Breton Island, Louisiana, August 2014

From August 11 to 31, 2014, the U.S. Geological Survey ( USGS ), in cooperation with the U.S. Fish and Wildlife Service ( USFWS ), conducted a geophysical survey to investigate the geologic controls on barrier island framework and long-term sediment transport offshore of Breton Island, Louisiana as part of a broader USGS study on Barrier Island Mapping ( BIM ). Additional details related to this activity can be found by searching the USGS's Coastal and Marine Geoscience Data System (CMGDS), for field activity 2014-317-FA (also known as 14BIM05). These surveys were funded through the USGS Coastal and Marine Geology Program ( CMGP ) and the Louisiana Outer Coast Early Restoration Project. This report serves as an archive of unprocessed digital chirp subbottom data, trackline maps, navigation files, Geographic Information System ( GIS ) files, Field Activity Collection System ( FACS ) logs, and formal Federal Geographic Data Committee ( FGDC ) metadata. Gained digital images of the seismic profiles are also provided. Refer to the Abbreviations page for explanations of acronyms and abbreviations used in this report. All chirp systems use an acoustic signal of continuously varying frequency; the EdgeTech 512i system used during this survey produced high-resolution, shallow-penetration (typically less than 50 milliseconds [ ms] ) profile images of sub-seafloor stratigraphy. The towfish contains a transducer that transmits and receives acoustic energy and typically is towed 1–2 meters ( m ) below the sea surface. As transmitted acoustic energy intersects density boundaries, such as the seafloor or sub-surface sediment layers, some energy is reflected back toward the transducer, received, and recorded by a Personal Computer ( PC) -based seismic acquisition system. This process is repeated at regular intervals (for example, 0.125 seconds ( s) ) and returned energy is recorded for a specific duration (for example, 50 ms ). In this way, a two-dimensional ( 2-D ) vertical image of the shallow geologic structure beneath the ship's track is produced. Figure 2 displays the acquisition geometry. Refer to table 1 for a summary of acquisition parameters and table 2 for trackline statistics. The archived trace data are in standard SEG Y rev. 0 format (Barry and others, 1975); the first 3,200 bytes of the card image header are in American Standard Code for Information Interchange ( ASCII ) format instead of Extended Binary Coded Decimal Interchange Code ( EBCDIC ) format. The SEG Y files are available on the DVD version of this report or online, downloadable via the USGS Coastal and Marine Geoscience Data System ( http://cmgds.marine.usgs.gov ). The data are also available for viewing using GeoMapApp ( http://www.geomapapp.org ) and Virtual Ocean ( http://www.virtualocean.org ) multi-platform open source software. The Web version of this archive does not contain the SEG Y trace files. To obtain the complete DVD archive, contact USGS Information Services at 1-888-ASK-USGS or infoservices@usgs.gov. The SEG Y files may be downloaded and processed with commercial or public domain software such as Seismic Unix ( SU ) (Cohen and Stockwell, 2010). See the How To Download SEG Y Data page for download instructions. The printable profiles are provided as Graphics Interchange Format ( GIF ) images processed and gained using SU software and can be viewed from the Profiles page or by using the links located on the trackline maps; refer to the Software page for links to example SU processing scripts.

Louisana↗
USGS1975

Polypodium sp. (Coelenterata) infection of paddlefish (Polyodon spathula) eggs

Parasitism of fish by coelenterates is rare and the first North American infection was reported in sturgeon (Hoffman et al., 1974, J Parasitol 60: 548-550). In the USSR parasitism of sturgeon (Acipenser sp.) eggs by the coelenterate Polypodium hydriforme Ussov 1885 has long been reported and investigated [Raikova, 1959, in Parasites of Freshwater Fish and the Biological Basis for their Control. Bull State Sci Res Inst Lake and River Fish. XLIX. Eng. transl. No. TT61-31056, O. T. S., U. S. Dep. Commerce, Washington, D. C., 1962, 235 p.: Raikova, 1973, (Proc Second Internat Symp on Cnidaria), Seto Marine Biological Laboratory 20: 165-173]. This report represents the first known infection of Polypodium in the paddlefish (Polyodon spathula).

Journal of Parasitology↗
USGS2023

Using multiscale environmental and spatial analyses to understand natural and anthropogenic influence on fish communities in four Canadian rivers

Science-based conservation of riverine fishes can be best targeted with specific information about spatial-ecological controls on the community, including anthropogenic stressors. Because anthropogenic stressors can originate at multiple spatial scales, we investigated the influence of natural and anthropogenic variables summarized within the reach, valley, and catchment on fish community composition along four river mainstems in Ontario, Canada. We used Redundancy Analyses (RDA) to explore models with multi- and single-scale variables on fish community composition. We used partial RDAs to differentiate the relative effects of variable types in multiscale models and to determine if spatial variables explained additional variation in fish community composition. Catchment variables accounted for the majority of explained variation in fish community composition in three of the four rivers, but instream habitat variables accounted for considerable variability in fish community composition in the two rivers that are highly fragmented by dams or naturally occurring rapids. Natural and human-derived fragmentation in rivers may reduce the influence of catchment controls, disrupt longitudinal gradients, and increase the influence of local instream habitat. Environmental variables that explained fish distribution had longitudinal or patchy spatial pattern within rivers, but spatial variables representing impediments to fish dispersal and proximity to receiving waterbodies failed to explain additional variation in fish community composition.

Ontario↗
USGS1979

Influence of dietary zinc on cataracts in rainbow trout (Salmo gairdneri)

The purpose of this study was to determine the cause of lens cataracts in hatchery trout fed diets containing white fish meal. After preliminary investigations, three experiments were conducted with fry of rainbow trout ( Salmo gairdneri ) that were fed practical-type diets containing either 40% herring meal (control diet) or 40% white fish meal with and without various mineral supplements. In all experiments, the trout fed herring meal grew well and had normal lenses. Those fed the diet containing white fish meal without supplement grew slowly and developed bilateral cataracts determined by using a slit-lamp biomicroscope. The severity of cataracts was increased by supplementing the diet with a mixture of minerals (phosphates and carbonates of calcium, sodium and potassium). Cataracts were prevented, however, with supplemental Na2EDTA or zinc but not by supplements of manganese, copper, iron or various other minerals. The metabolic alterations responsible for the zinc-deficiency cataract were not determined.

Journal of Nutrition↗
USGS2020

Aquatic invasive species in the Chesapeake Bay drainage—Research-based needs and priorities of U.S. Geological Survey partners and collaborators

Executive Summary The U.S. Geological Survey (USGS) is revising the Chesapeake Bay-based science plan to align it with recent U.S. Department of Interior and USGS science priorities that include, as stated in the plan, providing “an integrated understanding of the factors affecting fish habitat, fish health, and landscape conditions” in Chesapeake Bay and its watershed. A report of partner agencies’ needs and priorities related to aquatic invasive species (AIS) science was identified as an informational gap; a report would help to further development of the science program related to aquatic animal health and habitat. This objective was addressed through review of pertinent documentation and conversations with representatives of State, Federal, and regional agencies with vested interests in AIS management in Chesapeake Bay and the Chesapeake Bay drainage area, and this document was produced to summarize the related findings. All agencies and organizations (13) reported that AIS are of general concern, with most stakeholder groups reporting AIS-related issues to be of high priority, including invasive fishes and invertebrates, invasive plants, and microbes including aquatic animal pathogens. Invasive fishes are of great concern to all partner agencies. Channa argus (northern snakehead) and Ictalurus furcatus (blue catfish) are high priority and represent the two most named AIS of concern for these agencies. Nine of 10 stakeholder groups listed northern snakehead as a high priority species, and 6 listed blue catfish as a high priority species as well. Pylodictis olivaris (flathead catfish), invasive crayfish species , and dressenid mussels were also prioritized by multiple partner groups, each receiving specific mention by at least 3 of the 10 stakeholder groups in discussions or documents. Invasive carp, such as Hypophthalmichthys molatrix (silver carp), also received mention by multiple agencies (3 of the 10 stakeholder groups) because these fish represent priority AIS in nearby watersheds and a threat for introduction and dissemination within the Chesapeake Bay watershed from these neighboring regions. Invasive plants are among priority species, and Hydrilla verticillata (hydrilla) topped the list. Hydrilla was reported as a priority species by 5 of the stakeholder groups queried. Trapa natans and T. bispinosa (water chestnut), Phragmites australis (common reed), and Lythrum salicaria (purple loosestrife) were also among the aquatic invasive plants that were prioritized by multiple partner agencies. Multiple stakeholder groups (5 of the 10 groups) also considered Didymosphenia geminata (didymo) and various aquatic animal pathogens among their priority AIS for management considerations. Science needs that were recurrently indicated by stakeholders to support management of invasive species include Technology to enhance biosurveillance capability, such as reliable environmental DNA based detection methodology; Risk assessment modeling to forewarn of and prioritize AIS-related threats; Increased information and intervention methods related to vectors and pathways of AIS introductions; Increased information about the biology and life history of AIS, including information related to trophic interactions, health and disease, and distribution and abundance; and Potential applications of mitigation strategies, including genetically based biocontrol mechanisms. Potential next steps to address the science needs include Development of biosurveillance and risk assessment tools for identification of AIS in proactive management; Development of proactive management techniques to prevent AIS introductions through recognized vectors and pathways; Development of interagency biosurveillance programs to best utilize personnel, funds, and other resources among interested agencies and organizations; Investigations to address life history, consequences, and movement/dissemination of top priority invasive species in the region; Investigations to determine the potential for novel mitigation technologies, such as the application of synthetic biological (genetic) control methods; and Investigations with focus on emerging and high priority AIS in the region, including fishes (blue catfish, flathead catfish, northern snakehead), invertebrates (invasive crayfish and mollusks) and plants (hydrilla, water chestnut, phragmites).

Virginia, Maryland, Delaware, West Virginia, Penns↗
USGS2017

Light climate and dissolved organic carbon concentration influence species-specific changes in fish zooplanktivory

Dissolved organic carbon (DOC) in lakes reduces light penetration and limits fish production in low nutrient lakes, reportedly via reduced primary and secondary production. Alternatively, DOC and light reductions could influence fish by altering their visual feeding. Previous studies report mixed effects of DOC on feeding rates of zooplanktivorous fish, but most investigators tested effects of a single concentration of DOC against clear-water, turbid, or algal treatments. We used a controlled laboratory study to quantify the effects of a DOC gradient (3–19 mg L −1 ) on average light climate and the zooplankton feeding rate of 3 common, north temperate fishes. Light availability, which was inversely related to DOC concentration, had a positive and linear effect on zooplankton consumption by juvenile largemouth bass ( Micropterus salmoides ) and bluegill ( Lepomis macrochirus ), explaining 22% and 28% of the variation in consumption, respectively. By contrast, zooplankton feeding rates by fathead minnow ( Pimephales promelas ) were best predicted by a nonlinear, negative influence of light ( R 2 = 0.13). In bluegill feeding trials we found a general trend for positive selection of larger zooplankton (Cladocera and Chaoboridae); however, the light climate did not influence the selection of prey type. Largemouth bass selected for larger-bodied zooplankton, with weak evidence that selectivity for large Cladocera changed from negative to neutral selection based on electivity values across the light gradient. Our results suggest that the effect of DOC on the light climate of lakes may directly influence fish zooplanktivory and that this influence may vary among fish species.

Inland Waters↗
USGS2025

Expression of corticoid-regulatory genes in the gills of Atlantic salmon (Salmo salar) parr and smolt and during salinity acclimation

In teleost fishes, cortisol is the major corticoid and has both glucocorticoid and mineralocorticoid actions. However, how fish tissues discriminate between these distinct corticosteroid actions is unclear. In mammals, the major factors responsible for intracellular corticosteroid regulation are glucocorticoid receptors ( gr s) and the mineralocorticoid receptor ( mr ), but their role in osmoregulation of fish is unclear. 11β-hydroxysteroid dehydrogenases ( hsd11b s) control the levels of intracellular corticosteroids by converting from bioactive forms to inert forms. To investigate how Atlantic salmon ( Salmo salar ) respond to cortisol in different physiological or environmental conditions, we performed comparisons of parr and smolt, and osmotic challenge experiments to examine the physiological responses and gill transcript levels of genes underlying cortisol-signalling, including gr1 , gr2 , mr , hsd11b2 and hsd11b3 . Because cortisol may interact with growth hormone and prolactin during salinity changes, transcript levels encoding growth hormone receptors ( ghr1 , ghr2 ) and the prolactin receptor ( prlr ) were also examined. Hsd11b2 transcript levels in seawater-acclimated fish were consistently lower compared to fish acclimated to fresh water. After transfer to seawater, prlr transcript levels in fish significantly decreased and transcript levels of ghr1 , ghr2 and hsd11b3 showed no change or were slightly higher than those of freshwater control groups. Gr1 , gr2 and mr transcript levels were slightly but consistently higher in fish acclimated to fresh water relative to seawater. Our results indicate that changes in corticosteroid receptor and hsd11b2 transcript levels in the gills may be important mechanisms that regulate corticoid signals to achieve ion homeostasis in Atlantic salmon.

Journal of Fish Biology↗
USGS2012

Invasive zebra mussels ( Driessena polymorpha ) and Asian clams ( Corbicula fluminea ) survive gut passage of migratory fish species: implications for dispersal

The introduction and spread of invasive species is of great concern to natural resource managers in the United States. To effectively control the spread of these species, managers must be aware of the multitude of dispersal methods used by the organisms. We investigated the potential for survival through the gut of a migrating fish (blue catfish, Ictalurus furcatus ) as a dispersal mechanism for two invasive bivalves: zebra mussel ( Driessena polymorpha ) and Asian clam ( Corbicula fluminea ). Blue catfish (N = 62) were sampled over several months from Sooner Lake, Oklahoma, transported to a laboratory and held in individual tanks for 48 h. All fecal material was collected and inspected for live mussels. Survival was significantly related to water temperature in the lake at the time of collection, with no mussels surviving above 21.1 C°, whereas 12 % of zebra mussels (N = 939) and 39 % of Asian clams (N = 408) consumed in cooler water survived gut passage. This research demonstrates the potential for blue catfish to serve as a dispersal vector for invasive bivalves at low water temperatures.

Biological Invasions↗
USGS2023

Effects of nitrate and conductivity on embryo-larval fathead minnows

Nitrate concentrations have been rising in surface waters over the last century and now frequently exceed drinking water standards and environmental safety benchmarks globally. Health-wise, these trends are concerning because nitrate has been shown to disrupt endocrine function and developmental outcomes. The present study investigated potential sublethal effects of nitrate on developing fathead minnows. Fish were exposed from fertilization through 21 days postfertilization (dpf) to environmentally relevant concentrations of nitrate (0, 2, 5, 10, 25, or 100 mg/L NO 3 -N as NaNO 3 ). Nitrate effects on hatch timing, heart rate and rhythm at 3 dpf, growth through 21 dpf, swim bladder inflation timing and size, scoliosis, pericardial edema, and mortality were assessed. Because adding NaNO 3 increases water conductivity, two conductivity controls were included to match the ionic strength of the 10- and 100-mg/L NO 3 -N treatments. Increasing nitrate delayed posterior swim bladder (PSB) inflation in a dose-dependent manner, with possible inhibition of anterior swim bladder (ASB) inflation at higher doses, although nitrate did not affect swim bladder size. Conversely, nitrate did not affect hatch timing or cardiac endpoints at 3 dpf or induce pericardial edema or scoliosis, although there was a noted brood effect on these latter defects. As was observed with increasing nitrate, higher ion concentrations in the conductivity controls caused dose-dependent increases in fish body size at 21 dpf. Increased ionic strength also hastened ASB inflation independently of nitrate. As in other published studies, the observed delay in PSB inflation suggests that nitrate disrupts the thyroid axis and warrants further investigation. In addition, the present study supports the need for conductivity controls in nitrate toxicity studies to distinguish nitrate-specific effects. Environ Toxicol Chem 2023;00:1–13. Published 2023. This article is a U.S. Government work and is in the public domain in the USA.

Environmental Toxicology and Chemistry↗
USGS2020

Cortisol is an osmoregulatory and glucose-regulating hormone in Atlantic sturgeon, a basal ray-finned fish

Our current understanding of the hormonal control of ion regulation in aquatic vertebrates comes primarily from studies on teleost fishes, with relatively little information on more basal fishes. We investigated the role of cortisol in regulating seawater tolerance and its underlying mechanisms in an anadromous chondrostean, the Atlantic sturgeon ( Acipenser oxyrinchus ). Exposure of freshwater-reared Atlantic sturgeon to seawater (25 ppt) resulted in transient (1–3 day) increases in plasma chloride, cortisol and glucose levels and long-term (6–14 day) increases in the abundance of gill Na + /K + /2Cl − cotransporter (NKCC), which plays a critical role in salt secretion in teleosts. The abundance of gill V-type H + -ATPase, which is thought to play a role in ion uptake in fishes, decreased after exposure to seawater. Gill Na + /K + -ATPase activity did not increase in 25 ppt seawater, but did increase in fish gradually acclimated to 30 ppt. Treatment of Atlantic sturgeon in freshwater with exogenous cortisol resulted in dose-dependent increases in cortisol, glucose and gill NKCC and H + -ATPase abundance. Our results indicate that cortisol has an important role in regulating mechanisms for ion secretion and uptake in sturgeon and provide support for the hypothesis that control of osmoregulation and glucose by corticosteroids is a basal trait of jawed vertebrates.

Journal of Experimental Biology↗
USGS2020

Assessing establishment and growth of agricultural plantings on reservoir mudflats

Winter drawdowns in flood control reservoirs create expansive mudflats that lack the vegetation typical of littoral zones, which reduces the amount of structure available for fish habitat. This study investigated the feasibility of establishing agricultural plantings as a management action to ameliorate mudflats by providing structural cover following reservoir refilling. We tested cool-season annual grasses and clovers applied in several mixed and monoculture treatments that were sown on the mudflats of Enid Reservoir, Mississippi, during the winter drawdown in three consecutive years. Soil samples were taken for analysis of pH and macronutrients prior to planting. Plantings were monitored until the following spring to evaluate effectiveness of establishment through ground coverage, height, and stem density sampling. Plots were assigned a seeding treatment of either grasses (ryegrass Lolium spp. or triticale x Triticosecale sp.), clovers (balansa clover Trifolium michelianum or berseem clover Trifolium alexandrinum ), or both (mixed plantings) or left as an unseeded control. Differences among plant treatments were assessed via repeated measures analysis of variance and differences among means evaluated with Tukey's honestly significant difference test. Soil productivity within the study area was poor all 3 years. Grasses germinated both when disked into the soil and when top sown, while clover only germinated when disked. Plots seeded with grasses performed better than control plots with respect to stem density, height, and ground coverage, while plots seeded with grass and clover mixtures performed better than control plots only with respect to height, and plots seeded with only clover did not perform significantly better than control plots. Results serve as an evaluation of the efficacy of agricultural plant establishment on the mudflats of a flood control reservoir, inform the direction of future research, and identify considerations regarding the application of agricultural plantings as a management tool to create fish habitat.

Mississippi↗
USGS2008

Survival of cool and warm freshwater fish following chloramine-T exposure

Chloramine-T is presently available in the USA to control mortalities associated with bacterial gill disease or external columnaris only through an Investigational New Animal Drug Permit authorized by the US Food and Drug Administration (FDA). Its US approval hinges on FDA's acceptance of several key data, including those describing animal safety. Chloramine-T is presently applied in US aquaculture, by permit only, once daily on consecutive or alternate days for 1??h at 10 to 20??mg/L to control mortalities associated with bacterial gill disease or external columnaris. Our objective was to determine the safety of chloramine-T bath exposures at multiples of the proposed maximum treatment concentration (i.e., 0, 20, 60, 100, and 200??mg/L) administered on four consecutive days at 20????C to lake sturgeon Acipenser fulvescens, northern pike Esox lucius, and walleye Sander vitreum, or at 27????C to channel catfish Ictalurus punctatus, and largemouth bass Micropterus salmoides. All fish were tested as five to eight week old fry except for walleye and channel catfish which were tested as both fry and fingerling (fingerlings were at least four weeks older than the fry tested). Walleye and channel catfish were selected to evaluate the effects of life stage (fry vs. fingerling), temperature (walleye - 15, 20, or 25????C; channel catfish - 22, 27, or 32????C), exposure duration (60 vs. 180??min), and water chemistry (walleye only - reconstituted soft water vs. well water). Except for channel catfish fry, survival was significantly reduced only when fish were treated at 100 or 200??mg/L. Channel catfish fry survival was significantly reduced when exposed at 60??mg/L for 180??min at 27????C. Based on our mortality data, chloramine-T administered once daily for 60??min on four consecutive days at concentrations of up to 20??mg/L is not likely to adversely affect survival of cool or warmwater fish cultured in freshwater. Crown Copyright ?? 2007.

Aquaculture↗
USGS1980

Toxicity of five forest insecticides to cutthroat trout and two species of aquatic invertebrates

The Northern Rocky Mountain region has had scattered infestation of the western spruce budworm Christoneura occidentalis since the early 1900's (U.S. DEPARTMENT OF AGRICULTURE (USDA) 1976b). On the basis of aerial surveys in 1975, TUNNOCK et al. (1976), estimated that budworm defoliation occurred on 2,278,804 acres of six National Forests in Montana. Since the use of DDT was banned in 1972, there has been a need to develop alternative insecticides with the efficacy of DDT but without its environmental risk. These insecticides must be effective in controlling the budworm, but should not persist in the environment or be toxic to other organisms. The organophosphate and carbamate insecticides are relatively nonpersistent and generally present only a moderate hazard to fish when applied according to label recommendations. The USDA Forest Service has been investigating the effectiveness of these two classes of insecticides against the budworm, and the Columbia National Fisheries Research Laboratory of the U.S. Fish and Wildlife Service has been cooperating with the Forest Service conducted pilot control projects in eastern Montana in 1975 and 1976 to determine the efficacy and environmental impact of acephate, carbaryl, and trichlorfon in controlling the western budworm (USDA 1976 b). In 1975, a similar type project was carried out in Maine with aminocarb, fenitrothion, and trichlorfon (USDA 1976 a). Acephate, fenitrothion, and trichlorfon (organophosphate insecticides) and aminocarb and carbaryl (carbamate insecticides) were selected for toxicity tests against cutthroat trout ( Salmo clarki ), a stonefly ( Pteronarcella badia ), and a freshwater amphipod ( Gammarus pseudolimnaeus ) edemic in streams of the northern Rocky Mountains. Populations of cutthroat trout inhabit lakes and streams in the Rocky Mountains which include some of the most pristine habitat and fisheries in North America. Pteronarcella and Gammarus provide forage for cutthroat trout and feed on decaying vegetation in riffle areas in streams and rivers. Stonefly naiads and amphipods were selected as test organisms because of their importance as trout food and their wide distribution in mountain stream communities. We determined the effect of various water types representing different biogeographical areas in the Intermountain West on the toxicity of these five forest insecticides.

Bulletin of Environmental Contamination and Toxico↗
USGS1987

Methods of controlling growth of steelhead

Steelhead (Salmo gairdneri) held at 15°C and fully fed from demand feeders grow larger than desired by the time of their normal release into streams. The effect of limited feed intake on growth, body composition, and potential for migration to the sea was investigated. Dilution of feed by 10‐40% with sodium bentonite was not effective in reducing growth; the fish used the nutrients more efficiently and their growth was nearly equal to that of controls. Hand feeding of a decreased ration reduced growth rate, but about a 50% reduction in feed was required to effect the desired reduction in growth rate. Fish were able to compensate for fasting periods of short duration and significant growth reduction did not occur until the fasting periods were 4 d or longer. Alternate feeding and fasting periods of equal lengths produced the desired growth rate reduction. Estimated potential for emigration by these anadromous fish was not reduced by fasting periods as long as 28 d.

Progressive Fish-Culturist↗
USGS2014

Methylmercury-induced changes in gene transcription associated with neuroendocrine disruption in largemouth bass (Micropterus salmoides)

Methyl-mercury (MeHg) is a potent neuroendocrine disruptor that impairs reproductive processes in fish. The objectives of this study were to (1) characterize transcriptomic changes induced by MeHg exposure in the female largemouth bass (LMB) hypothalamus under controlled laboratory conditions, (2) investigate the health and reproductive impacts of MeHg exposure on male and female largemouth bass (LMB) in the natural environment, and (3) identify MeHg-associated gene expression patterns in whole brain of female LMB from MeHg-contaminated habitats. The laboratory experiment was a single injection of 2.5 μg MeHg/g body weight for 96 h exposure. The field survey compared river systems in Florida, USA with comparably lower concentrations of MeHg (Wekiva, Santa Fe, and St. Johns Rivers) in fish and one river system with LMB that contained elevated concentrations of MeHg (St. Marys River). Microarray analysis was used to quantify transcriptomic responses to MeHg exposure. Although fish at the high-MeHg site did not show overt health or reproductive impairment, there were MeHg-responsive genes and pathways identified in the laboratory study that were also altered in fish from the high-MeHg site relative to fish at the low-MeHg sites. Gene network analysis suggested that MeHg regulated the expression targets of neuropeptide receptor and steroid signaling, as well as structural components of the cell. Disease-associated gene networks related to MeHg exposure, based upon expression data, included cerebellum ataxia, movement disorders, and hypercalcemia. Gene responses in the CNS are consistent with the documented neurotoxicological and neuroendocrine disrupting effects of MeHg in vertebrates.

General and Comparative Endocrinology↗
USGS2012

Molecular responses differ between sensitive silver carp and tolerant bighead carp and bigmouth buffalo exposed to rotenone

Some species of fish are more tolerant of rotenone, a commonly used non-specific piscicide, than others. This species-specific tolerance to rotenone has been thought to be associated with the uptake and the efficiency at which the chemical is detoxified. However, rotenone stimulates oxidative stress and superoxides, which are also toxic. Understanding the modes in which fish physiologically respond to rotenone is important in developing improved protocols for its application in controlling aquatic nuisance species. Using a molecular approach, we investigated the physiological and molecular mechanisms of rotenone resistance. Species-specific responses were observed when rotenone-sensitive silver, Hypophthalmichthys molitrix, and both rotenone-resistant bighead carp, Hypophthalmichthys nobilis, and bigmouth buffalo, Ictiobus cyprinellus, were exposed to rotenone. Rotenone levels in plasma were highest 90 min after exposure in both silver carp and bigmouth buffalo, but bigmouth buffalo tolerated over twice the burden (ng mL -1 g -1 ) than silver carp. Expression of genes related with detoxification ( cyp1a and gst ) increased in silver carp, but either decreased or remained the same in bighead carp. Genes linked with oxidative stress in the cytosol ( gpx , cat and sod1 ) and hsp70 increased only in silver carp after a 6-h exposure. Expression of genes associated with oxidative stress in the mitochondria ( sod2 and ucp2 ) differed between silver carp and bighead carp. Expression of sod2 changed minimally in bighead carp, but expression of ucp2 linearly increased to nearly 85-fold of the level prior to exposure. Expression of sod2 and ucp2 did not change until 6 h in silver carp. Use of sod1 and sod2 to combat oxidative stress results in hydrogen peroxide production, while use of ucp2 produces nitric oxide, a chemical known to inhibit apoptosis. We conclude that the mechanism at which a fish handles oxidative stress plays an important role in the tolerance to rotenone.

Fish Physiology and Biochemistry↗
USGS2008

Ethoxyresorufin-O-deethylase (EROD) activity in fish as a biomarker of chemical exposure

This review compiles and evaluates existing scientific information on the use, limitations, and procedural considerations for EROD activity (a catalytic measurement of cytochrome P4501A induction) as a biomarker in fish. A multitude of chemicals induce EROD activity in a variety of fish species, the most potent inducers being structural analogs of 2,3,7,8-tetracholordibenzo- p -dioxin. Although certain chemicals may inhibit EROD induction/activity, this interference is generally not a drawback to the use of EROD induction as a biomarker. The various methods of EROD analysis currently in use yield comparable results, particularly when data are expressed as relative rates of EROD activity. EROD induction in fish is well characterized, the most important modifying factors being fish species, reproductive status and age, all of which can be controlled through proper study design. Good candidate species for biomonitoring should have a wide range between basal and induced EROD activity (e.g., common carp, channel catfish, and mummichog). EROD activity has proven value as a biomarker in a number of field investigations of bleached kraft mill and industrial effluents, contaminated sediments, and chemical spills. Research on mechanisms of CYP1A-induced toxicity suggests that EROD activity may not only indicate chemical exposure, but also may also precede effects at various levels of biological organization. A current research need is the development of chemical exposure-response relationships for EROD activity in fish. In addition, routine reporting in the literature of EROD activity in standard positive and negative control material will enhance confidence in comparing results from different studies using this biomarker.

Critical Reviews in Toxicology↗
USGS2012

Abiotic and biotic controls of cryptobenthic fish assemblages across a Caribbean seascape

The majority of fish studies on coral reefs consider only non-cryptic species and, despite their functional importance, data on cryptic species are scarce. This study investigates inter-habitat variation in Caribbean cryptobenthic fishes by re-analysing a comprehensive data set from 58 rotenone stations around Buck Island, U.S. Virgin Islands. Boosted regression trees were used to associate the density and diversity of non-piscivorous cryptobenthic fishes, both in the entire data set and on reef habitats alone, with 14 abiotic and biotic variables. The study also models the habitat requirements of the three commonest species. Dead coral cover was the first or second most important variable in six of the eight models constructed. For example, within the entire data set, the number of species and total fish density increased approximately linearly with increasing dead coral cover. Dead coral was also important in multivariate analyses that discriminated 10 assemblages within the entire data set. On reef habitats, the number of species and total fish density increased dramatically when dead coral exceeded ~55 %. Live coral cover was typically less important for explaining variance in fish assemblages than dead coral, but live corals were important for maintaining high fish diversity. Coral species favoured by cryptobenthic species may be particularly susceptible to mortality, but dead coral may also provide abundant food and shelter for many fishes. Piscivore density was a key variable in the final models, but typically increased with increasing cryptobenthic fish diversity and abundance, suggesting both groups of fishes are responding to the same habitat variables. The density of territorial damselfishes reduced the number of cryptobenthic fish species on reef habitats. Finally, habitats delineated by standard remote sensing techniques supported distinct cryptobenthic fish assemblages, suggesting that such maps can be used as surrogates of general patterns of cryptic fish biodiversity in conservation planning.

Coral Reefs↗