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At least 163 records · Page 9Linked to original sources

Strategic habitat conservation for beach mice: Estimating management scenario efficiencies

The Perdido Key beach mouse ( Peromyscus polionotus trissyllepsis ), Choctawhatchee beach mouse ( P. p. allophrys ), and St. Andrew beach mouse ( P. p. peninsularis ) are 3 federally endangered subspecies that inhabit coastal dunes of Alabama and Florida, USA. Conservation opportunities for these subspecies are limited and costly. Consequently, well‐targeted efforts are required to achieve their downlisting criteria. To aid the development of targeted management scenarios that are designed to achieve downlisting criteria, we developed a Bayesian network model that uses habitat characteristics to predict the probability of beach mouse presence at a 30‐m resolution across a portion of the Florida Panhandle. We then designed alternative management scenarios for a variety of habitat conditions for coastal dunes. Finally, we estimated how much area is needed to achieve the established downlisting criterion (i.e., habitat objective) and the amount of effort needed to achieve the habitat objective (i.e., management efficiency). The results suggest that after 7 years of post‐storm recolonization, habitat objectives were met for Perdido Key (within its Florida critical habitat) and Choctawhatchee beach mice. The St. Andrew beach mouse required 5.14 km 2 of additional critical habitat to be protected and occupied. The St. Andrew beach mouse habitat objective might be achieved by first restoring protected critical habitat to good dune conditions and then protecting or restoring the unprotected critical habitat with the highest predicted probability of beach mouse presence. This scenario provided a 28% increase in management efficiency compared to a scenario that randomly protected or restored undeveloped unprotected critical habitat. In total, when coupled with established downlisting criteria, these quantitative and spatial decision support tools could provide insight into how much habitat is available, how much more is needed, and targeted conservation or restoration efforts that might efficiently achieve habitat objectives.

Alabama, Florida↗

Adaptive capacity of alpine fauna in Australia

Climate change is leading to rapid and unprecedented changes to Australia's alpine ecosystems. The impacts of these changes at a species level are not fully understood, making it challenging to holistically assess the impacts of a changing climate in these ecosystems. To address this gap, we combine a literature review with expert opinion to conduct individual adaptive capacity analyses for 29 species of Australia's alpine animals. We conduct these analyses using a published framework which encompasses key themes, such as life history, capacity for movement, genetic variation, and distribution. Our results highlight diversity in the adaptive capacity of Australia's alpine animals and suggest that climate change will impact even closely related species in surprisingly disparate ways. Our study also provides an example of how to overcome perceived data deficiency and support conservation practitioners with what is known. We have created an important resource for land managers in Australia's Alps that systematically integrates diverse data to provide insight into the mechanisms behind the vulnerability of some of Australia's most threatened species to climate change. The evidence this study collates should be considered in conservation planning to ensure species-level natural history information is included in decisions about management actions to mitigate climate change impacts.

Conservation Science and Practice↗

A Lake Charr pangenome reveals highly conserved Ohnologs as drivers of phenotypic diversity

Whole-genome duplication (WGD) is hypothesized to spur evolutionary diversification by producing genome-wide duplicate gene sets (Ohnologs) that are initially functionally redundant but can diverge markedly as the effects of relaxed selection accumulate over time. However, the underlying mechanisms remain unclear, in part because genomic studies often reconstruct Ohnolog evolution over millions of years, during which subsequent mutations can obscure deep-time signals. Investigating the relationship between Ohnolog evolution and diversification on a contemporary timescale offers clearer insights. We explore this relationship in Lake Charr ( Salvelinus namaycush ), where ∼10% of genes are retained highly conserved polyploid duplicates following the Salmonid-Specific Fourth Round WGD. Using 31 chromosome-level assemblies of Lake Charr from morphologically and ecologically diverse populations, joined into a pangenome graph, we characterized 189,555 structural variants (SVs) that were significantly less likely to affect genes retained as sequence-conserved Ohnolog pairs, nuancing the hypothesis that gene redundancy, relaxed selection, and functional diversification are intertwined. However, we found that SVs affecting such conserved Ohnologs may be potent drivers of adaptive evolution. Notably, we identified a putative 938-Kb interchromosomal translocation containing 25 genes with highly conserved Ohnologs in a paralogous (but untranslocated) genomic block. This putative translocation appears to have facilitated Ohnolog divergence in ankrd11 and hp , genes putatively linked to craniofacial and lipid metabolic diversity in sympatric Lake Superior morphs. This research reveals that conserved Ohnologs previously presumed to be redundant remain a reservoir for adaptive change.

BioRxiv↗

Carcass size and ground substrate drive detection rates of avian carcasses by human surveyors and a dog team

Accurate avian mortality estimates are essential for understanding anthropogenic impacts to bird populations and informing conservation strategies. Carcass surveys are commonly conducted by human surveyors or by detection dogs, but the factors influencing surveyor detection abilities have not been fully explored. In this study, we conducted two years of detection trials in the semi-arid high desert of southern New Mexico, USA, testing 27 human surveyors and one conservation detection dog across 1096 trials with 238 carcasses representing 50 avian species. We directly compared detection abilities between surveyor types (human and dog) and identified key factors influencing detection probabilities. The conservation detection dog exhibited a significantly higher detection probability (mean = 0.87) than human surveyors (mean = 0.49, individuals ranged 0.25–0.71), consistent with previous studies. Detection probabilities for both surveyor types were influenced by carcass size and ground substrate; detection probability was higher for larger carcasses and areas with lower vegetative complexity. We discuss our results in the context of common tradeoffs faced by managers in designing carcass surveys and how guidance may vary under different scenarios. Broadly, our study provides valuable insight that can enhance wildlife mortality monitoring, ensuring more accurate mortality estimates to inform management and conservation efforts.

New Mexico↗

Bat activity patterns relative to temporal and weather effects in a temperate coastal environment

The northeastern and mid-Atlantic coasts of the United States are important summer maternity habitat and seasonal migratory corridors for many species of bats. Additionally, the effects of weather on bat activity are relatively unknown beyond coarse nightly scales. Using acoustic detectors, we assessed nightly and hourly activity patterns for eight species of bats over 21 consecutive months at Fire Island National Seashore, New York. The site is an important bat conservation area because it hosts one of the few confirmed northern long-eared bat ( Myotis septentrionalis ) maternity colonies in the region despite their widespread extirpation due to white-nose syndrome (WNS). There have been no reported captures of little brown bats ( M. lucifugus ), Indiana bats ( M. sodalis ), or tri-colored bats ( Perimyotis subflavus ) at the site post-WNS. Overall, we found mean hourly temperature, time since sunset, day of year, and year to be the most important predictors of bat activity levels for all examined species. Most non-hibernating, migratory species in our study demonstrated a positive relationship to mean temperature at the hourly timescale, whereas cave-hibernating bats tended to show a negative relationship to mean temperature during the time of year when they are expected to be active. Although most bat activity occurred in the late spring through early autumn, peaking in summer, some activity occurred periodically in the winter months, mostly attributable to the big brown bat ( Eptesicus fuscus ) and silver-haired bat ( Lasionycteris noctivigans ) phonic group. Unexpectedly, relationships of bat activity to wind and precipitation were largely equivocal. Initial presence (as early as March 30) and departure (between November 1–4) for northern long-eared bats at our study area occurred earlier in the spring and later in the fall than occurs for inland populations, suggesting that the species overwinters on Long Island rather than at inland karst caves or mines. A peak in spring activity characteristic of migratory behavior in the central Appalachians and Atlantic Coast was not observed at Fire Island, although Eastern red bats ( Lasiurus borealis ) and hoary bats (L. cinereus ) – both migratory species – did show a notable rise in activity in the late summer and early fall, suggesting these populations may migrate to and from Fire Island. Understanding the temporal and weather relationships to bat activity in this coastal environment may have important implications for tailoring more effective conservation and management strategies by identifying optimal timing for surveys, tracking bats during peak migratory windows, and providing insights that minimizes impacts to extant bats from activities such as wind-energy development or land management, i.e., forestry.

New York↗

Population dynamics of the Cui-ui of Pyramid Lake, Nevada: A Potamodromous catostomid subject to failed reproduction

Fishes of the Truckee River basin (California and Nevada) evolved in an aquatic system that has been episodically diminished by extended drought. For potamodromous species, such as the endangered Cui-ui endemic to Pyramid Lake, Nevada, prehistoric episodic severe drought presumably led to periods of failed reproduction due to restricted access to spawning habitat. The response of the Cui-ui population to more recent failed reproduction caused by anthropogenic activity was studied to learn how to manage this species through periods of spawning disruption. Adult Cui-ui survival averaged 91% and 89% for females and males, respectively, in drought years when spawning migrations were either precluded or few fish migrated because of no or low stream flow. In each of 2 years when stream access was precluded, the adult survival was nearly 100% suggesting that Cui-ui survival is extended in the absence of a spawning migration. Survival averaged 62% and 60% for females and males, respectively, in years of spawning migrations. Strong predominant year-classes developed in the year immediately following a period of failed reproduction, indicating the species’ capacity for population rebound. Year-class predominance persisted for 6–10 years and through years of low survival associated with migration years, and this predominance is probably due, in part, to a diverse age at maturity. Contemporary water diversions from the Truckee River provided the opportunity to study the response of the Cui-ui population to years of failed reproduction. A projected drier Truckee River basin associated with global climate change will test the Cui-ui’s adaptive capacity to endure periods of reproductive failure. This study is aimed at assisting Cui-ui managers in conserving the species in this highly regulated and changing system. The study also adds insight into the prehistoric population dynamics of a potamodromous species in the arid western United States subject to wide fluctuations in annual precipitation and water availability.

California, Nevada↗

The silent threat of low genetic diversity

Across the Caribbean, protected coastal waters have served as primary feeding and breeding grounds for the endangered Antillean manatee. Unfortunately, these same coastal waters are also a popular “habitat” for humans. In the past, the overlap between human and manatee habitat allowed for manatee hunting and threatened the survival of these gentle marine mammals. Today, however, threats are much more inadvertent and are often related to coastal development, degraded habitats and boat strikes. In the state of Florida, decades of research on the species’ biological needs have helped conservationists address threats to its survival. For example, low wake zones and boater education have protected manatees from boat strikes, and many of their critical winter refuges are now protected. The Florida population has grown steadily, thus increasing from approximately 1,200 in 1991 to more than 5,000 in 2010. It is conceivable that in Florida manatees may one day be reclassified as “threatened” rather than “endangered.” Yet, in other parts of the Caribbean, threats still loom. This includes small, isolated manatee populations found on islands that can be more susceptible to extinction and lack of genetic diversity. To ensure the species’ long-term viability, scientists have turned their sights to the overall population dynamics of manatees throughout the Caribbean. Molecular genetics has provided new insights into long-term threats the species faces. Fortunately, the emerging field of conservation genetics provides managers with tools and strategies for protecting the species’ long-term viability.

livebetter Magazine↗

Science to support conservation action in a large river system: The Willamette River, Oregon, USA

Management and conservation efforts that support the recovery and protection of large rivers are daunting, reflecting the complexity of the challenge and extent of effort (in terms of policy, economic investment, and spatial extent) needed to afford measurable change. These large systems have generally experienced intensive development and regulation, compromising their capacity to respond to disturbances such as climate change or wildfire. Functionally, large river and basin management require insights gained from social, ecological, geophysical, and hydrological sciences. This multi-disciplinary perspective can unveil the integrated relationship between a river network's biotic community and seasonally variable environmental conditions that are often influenced by human activities. Large rivers and their basins are constantly changing due to anthropogenic influences and as climate modifies patterns of temperature and precipitation. Because of these factors, the state of knowledge must advance to address changing conditions. The Willamette River, in western Oregon, USA, is a prime example of a basin that has experienced significant degradation and investment in rehabilitation in recent decades. Innovative science has facilitated development of fine-scale, spatially extensive datasets and models that can generate targeted conservation and rehabilitation actions that are prioritized across the entire river network. This prioritization allows investment decisions to be driven by site-specific conditions while simultaneously considering potentials for ecological improvement. Here, we review hydrologic, geomorphic, ecologic, and social conditions in the Willamette River basin through time—including pre-settlement, river development, and contemporary periods—and offer a future vision for consideration. Currently, detailed information about fish populations and habitat, hydrologic conditions, geomorphology, water quality, and land use can be leveraged to make informed decisions about protection, rehabilitation, and development. The time is ripe for strategic management and goal development for the entire Willamette River, and these efforts can be informed by comprehensive science realized through established institutions (e.g., public agencies, non-profit watershed groups, Tribes, and universities) focused on conservation and management. The approaches to science and social-network creation that were pioneered in the Willamette River basin offer insights into the development of comprehensive conservation-based planning that could be implemented in other large river systems globally.

Oregon↗

Whitebark pine mortality related to white pine blister rust, mountain pine beetle outbreak, and water availability

Whitebark pine ( Pinus albicaulis ) forests in the western United States have been adversely affected by an exotic pathogen ( Cronartium ribicola , causal agent of white pine blister rust), insect outbreaks ( Dendroctonus ponderosae , mountain pine beetle), and drought. We monitored individual trees from 2004 to 2013 and characterized stand-level biophysical conditions through a mountain pine beetle epidemic in the Greater Yellowstone Ecosystem. Specifically, we investigated associations between tree-level variables (duration and location of white pine blister rust infection, presence of mountain pine beetle, tree size, and potential interactions) with observations of individual whitebark pine tree mortality. Climate summaries indicated that cumulative growing degree days in years 2006–2008 likely contributed to a regionwide outbreak of mountain pine beetle prior to the observed peak in whitebark mortality in 2009. We show that larger whitebark pine trees were preferentially attacked and killed by mountain pine beetle and resulted in a regionwide shift to smaller size class trees. In addition, we found evidence that smaller size class trees with white pine blister rust infection experienced higher mortality than larger trees. This latter finding suggests that in the coming decades white pine blister rust may become the most probable cause of whitebark pine mortality. Our findings offered no evidence of an interactive effect of mountain pine beetle and white pine blister rust infection on whitebark pine mortality in the Greater Yellowstone Ecosystem. Interestingly, the probability of mortality was lower for larger trees attacked by mountain pine beetle in stands with higher evapotranspiration. Because evapotranspiration varies with climate and topoedaphic conditions across the region, we discuss the potential to use this improved understanding of biophysical influences on mortality to identify microrefugia that might contribute to successful whitebark pine conservation efforts. Using tree-level observations, the National Park Service-led Greater Yellowstone Interagency Whitebark Pine Long-term Monitoring Program provided important ecological insight on the size-dependent effects of white pine blister rust, mountain pine beetle, and water availability on whitebark pine mortality. This ongoing monitoring campaign will continue to offer observations that advance conservation in the Greater Yellowstone Ecosystem.

Ecosphere↗

Model-based clustering reveals patterns in central place use of a marine top predator

Satellite telemetry data are commonly used to quantify habitat selection, examine animal movements, and delineate home ranges. These data also contain valuable information concerning dens, nests, roosts, and other central places that are often associated with important life history events and may exhibit unique characteristics; however, using satellite telemetry data to study central places is complicated by common nuances like locational error and animal movement. We coupled a novel modeling framework that accounts for these nuances with an Argos satellite telemetry dataset to examine the spatiotemporal behavior associated with harbor seal haul-out sites on Kodiak Island, Alaska, USA. The methodology incorporates an observation model that accommodates multiple sources of uncertainty in telemetry data and a flexible Bayesian nonparametric model to uncover latent clustering in the telemetry locations. We also contribute extensions to examine the effect of covariates on site selection and to obtain population-level inference concerning central place use. Harbor seal haul-out sites generally occurred in inlets and bays, areas that are isolated from the open water of the Gulf of Alaska. Most individuals selected haul-out sites that were protected from wave exposure. The effects of bathymetry and shoreline complexity on haul-out site selection were variable among individual seals, as were the effects of time of day, time since low tide, and day of year on temporal patterns of haul-out use. As repositories of satellite telemetry data on a wide variety of species accumulate, so do opportunities for using this information to learn about the locations of central places, as well as the temporal patterns in their use. The model-based approach we describe offers a practical and rigorous means for gaining insight concerning these sensitive locations, knowledge of which is important for the effective management and conservation of many species.

Alaska↗

Comparison of microscopy and metabarcoding to identify pollen used by the critically endangered rusty patched bumble bee, Bombus affinis

Taxonomic analysis of pollen collected by bees can provide insights into their host plant use, providing information about the plant species selected for targeted conservation strategies. The two main identification approaches used are morphological analysis of pollen samples affixed to microscope slides (i.e., microscopic palynology) and molecular analysis of samples. Both methods are widely used for freshly collected materials and have been compared in multiple studies, yet their application to archived samples remains to be explored. Archived samples may be particularly useful for the study of rare or protected species, particularly when historical foraging patterns are uncertain. We used both methods to analyse pollen collected by the endangered rusty patched bumble bee species, Bombus affinis Cresson, applied to museum-archived specimens. Pollen samples were removed from the corbiculae of bumble bees originally collected in Michigan between 1914 and 1974. Samples included 24 rusty patched bumble bees each with large pollen loads on both corbiculae, allowing for pollen from the same bee to be analysed using both methods. DNA metabarcoding detected more plant taxa than light microscopy, and DNA barcoding also had higher taxonomic resolution when compared to taxa determined using light microscopy. In many instances, pollen could only be confidently identified to tribe or family with light microscopy. Discrepancy between methods decreased when taxa identified via DNA metabarcoding were binned into ecologically relevant groups corresponding to those identified using light microscopy. Although binning demonstrated smaller within-method variance, there was still minimal correspondence between the two methods. Results indicate there are benefits and biases unique to each method and highlight the utility of binning taxonomic results to morphological or ecological groupings.

Insect Conservation and Diversity↗

Characteristics of foraging sites and protein status in wintering muskoxen: insights from isotopes of nitrogen

Identifying links between nutritional condition of individuals and population trajectories greatly enhances our understanding of the ecology, conservation, and management of wildlife. For northern ungulates, the potential impacts of a changing climate to populations are predicted to be nutritionally mediated through an increase in the severity and variance in winter conditions. Foraging conditions and the availability of body protein as a store for reproduction in late winter may constrain productivity in northern ungulates, yet the link between characteristics of wintering habitats and protein status has not been established for a wild ungulate. We used a non‐invasive proxy of protein status derived from isotopes of N in excreta to evaluate the influence of winter habitats on the protein status of muskoxen in three populations in Alaska (2005–2008). Multiple regression and an information‐theoretic approach were used to compare models that evaluated the influence of population, year, and characteristics of foraging sites (components of diet and physiography) on protein status for groups of muskoxen. The observed variance in protein status among groups of muskoxen across populations and years was partially explained (45%) by local foraging conditions that affected forage availability. Protein status improved for groups of muskoxen as the amount of graminoids in the diet increased (−0.430 ± 0.31, β± 95% CI) and elevation of foraging sites decreased (0.824 ± 0.67). Resources available for reproduction in muskoxen are highly dependent upon demographic, environmental, and physiographic constraints that affect forage availability in winter. Due to their very sedentary nature in winter, muskoxen are highly susceptible to localized foraging conditions; therefore, the spatial variance in resource availability may exert a strong effect on productivity. Consequently, there is a clear need to account for climate–topography effects in winter at multiple scales when predicting the potential impacts of climatic shifts on population trajectories of muskoxen.

Oikos↗

Salmon data mobilization

Despite substantial research and conservation efforts, many salmon populations are in decline. Globally, salmon research is not delivering effective decision support products to help managers apply research insights as informed management actions. Data Mobilization (DM) is a key step towards building the wider evidence base required to deliver accountable, reliable, and usable scientific advice to managers. Best practices for DM are being adopted throughout the scientific community but have not permeated deeply into the culture of salmon research and conservation. To address this, we present a strategy for Salmon Data Mobilization (SDM). This strategy defines three spheres of agencies and practitioners that must interact to advance SDM: (1) authoritative bodies that can create policies to support SDM uptake; (2) agencies that can promote, fund, and implement those policies; and (3) the broad salmon community of practice that can support uptake of SDM within focused interest groups. We sketch a future for SDM and propose functional changes required to improve it throughout the community.

North Pacific Anadromous Fish Commission Bulletin↗

Using a genetic mixture model to study phenotypic traits: Differential fecundity among Yukon river Chinook Salmon

Fecundity is a vital population characteristic that is directly linked to the productivity of fish populations. Historic data from Yukon River (Alaska) Chinook salmon Oncorhynchus tshawytscha suggest that length‐adjusted fecundity differs among populations within the drainage and either is temporally variable or has declined. Yukon River Chinook salmon have been harvested in large‐mesh gill‐net fisheries for decades, and a decline in fecundity was considered a potential evolutionary response to size‐selective exploitation. The implications for fishery conservation and management led us to further investigate the fecundity of Yukon River Chinook salmon populations. Matched observations of fecundity, length, and genotype were collected from a sample of adult females captured from the multipopulation spawning migration near the mouth of the Yukon River in 2008. These data were modeled by using a new mixture model, which was developed by extending the conditional maximum likelihood mixture model that is commonly used to estimate the composition of multipopulation mixtures based on genetic data. The new model facilitates maximum likelihood estimation of stock‐specific fecundity parameters without first using individual assignment to a putative population of origin, thus avoiding potential biases caused by assignment error. The hypothesis that fecundity of Chinook salmon has declined was not supported; this result implies that fecundity exhibits high interannual variability. However, length‐adjusted fecundity estimates decreased as migratory distance increased, and fecundity was more strongly dependent on fish size for populations spawning in the middle and upper portions of the drainage. These findings provide insights into potential constraints on reproductive investment imposed by long migrations and warrant consideration in fisheries management and conservation. The new mixture model extends the utility of genetic markers to new applications and can be easily adapted to study any observable trait or condition that may vary among populations.

Transactions of the American Fisheries Society↗

Wyoming Landscape Conservation Initiative—A case study in partnership development

The Wyoming Landscape Conservation Initiative (WLCI) is a successful example of collaboration between science and natural resource management at the landscape scale. In southwestern Wyoming, expanding energy and mineral development, urban growth, and other changes in land use over recent decades, combined with landscape-scale drivers such as climate change and invasive species, have presented compelling challenges to resource managers and a diverse group of Federal, State, industry, and non-governmental organizations, as well as citizen stakeholders. To address these challenges, the WLCI was established as a collaborative forum and interagency partnership to develop and implement science-based conservation actions. About a decade after being established, this report documents the establishment and history of the WLCI, focusing on the path to success of the initiative and providing insights and details that may be useful in developing similar partnerships in other locations. Not merely retrospective, the elements of the WLCI that are presented herein are still in play, still evolving, and still contributing to the resolution of compelling conservation challenges in the Western United States. The U.S. Geological Survey has developed many successful longstanding partnerships, of which the WLCI is one example. “As the Nation’s largest water, earth, and biological science and civilian mapping agency, the U.S. Geological Survey collects, monitors, analyzes, and provides scientific understanding about natural resource conditions, issues, and problems. The diversity of our scientific expertise enables us to carry out large-scale, multi-disciplinary investigations and provide impartial scientific information to resource managers, planners, and other customers” (U.S. Geological Survey, 2016).

Wyoming↗

White light reduces flight height of birds in low-density migration corridor

Artificial light at night (ALAN) has been shown to influence the behavior of migratory birds, yet how different light spectra modulate these effects remains unclear. We conducted a field experiment for 31 nights at a remote site in northern Colorado, USA, using 2 800-W light-emitting diode (LED) floodlights with white, red, amber, and blue lighting treatments during fall migration in 2023 and 2024. Using a vertical-looking radar system, we quantified avian in-flight responses in migration traffic rate, flight height, and flight direction between paired control periods with no light and periods with lighting treatments. Although we found no effects of our treatments on migration traffic rate and weak effects on flight direction, we found that broad-spectrum white light with emissions across a 400–600 nm spectrum reduced mean avian flight height by an estimated 22.57 m (±10.53 SE) compared to dark periods, and this response was stronger than red and blue lighting treatments. Our ability to detect behavior changes from a point source in a rural, low-density migration system supports the notion that the effects of artificial light at night on bird behavior may be more pervasive than is often recognized, and that this varies by ALAN spectra. Beyond providing insight into avian orientation and in-flight behavior, our results have implications for future research, the conservation management of artificial light at night, and lighting designs that minimize effects on migratory birds.

Colorado↗

Pit tag application in native freshwater mussels: Case studies across small, medium, and large rivers

Since their first use in the mid-1980s, external passive integrated transponder (PIT) tags have facilitated innovative investigations into multiple biological traits of animals. For native freshwater mussels, PIT tags are frequently used in capture-mark-recapture applications because they allow repeated, noninvasive sampling, are easy to apply, have high retention rates, and have negligible short-term effects on growth and survival. Because of these traits, resource managers and scientists are using PIT-tagged animals to estimate survival and movement of mussels associated with restoration efforts. However, consistency is limited in how PIT tags are affixed, monitored, and reported. Thus, our objectives were to (1) share our collective experiences in PIT tagging mussels across three case studies in small, medium, and large rivers and (2) propose guidelines for tagging and reporting data from PIT tag studies with native freshwater mussels to facilitate comparisons across future studies. The number of studies that have marked mussels with PIT tags has increased over the past 10 years. The ability to detect mussels using PIT tags has substantially advanced research in three areas of mussel ecology: (1) estimating vital rates (e.g., growth and survival), (2) tracking movements and behaviors of captively propagated, wild, and translocated individuals, and (3) improving our understanding of life history traits, such as reproductive timing. Each case study offers insights on tagging methods, tag loss, tag retention, and monitoring frequency across multiple species that range in conservation status from common to rare. We conclude with best-practice guidelines for placing PIT tags on freshwater mussels and a list of variables that could be reported in future studies to facilitate cross-system comparisons.

Freshwater Mollusk Biology and Conservation↗

Comparison of beaver density estimates from aerial surveys of waterways versus transects

Historic beaver-sign (Castor canadensis) survey flights were often conducted over waterways to maximize beaver detections. However, densities determined from strip transect surveys are more useful to compare across and within study areas than waterway indices based on observations per distance flown because transects are more representative of the wider landscape. Yet, it is unknown if, and to what extent, aerial waterway surveys are reflective of transect densities. I conducted aerial surveys for active beaver sign each fall during 2015–2018 over two waterway routes and two corresponding strip-transect routes. The simple linear regression of transect densities on waterway densities (n=8) yielded a reasonable (R2=0.79) preliminary equation for converting historic waterway data to transect densities. Additionally, visual inspection indicated that converted waterway densities reasonably reflected the trend in transect densities in an area where the wider habitat was similar in terms of beaver harvest, land use, and proportion of water features. Although trend was well-reflected, individual waterway densities in this area were only 57-75% of transect densities. In other areas, where water features were limited, visual inspection suggested the trend of waterway densities was less reflective of transect density trend and individual waterway densities overestimated transect densities (up to 309%). Nevertheless, while transect densities are better for comparisons within and across study areas, waterway surveys are still important for timely and specific within-study area insights. This research provides useful benchmark examples of reliability regarding waterway observation indices converted to densities for conservation, research, and management of beaver and their ecosystems. Because these conclusions are based on a small sample, additional research is recommended to better define this relationship especially in areas with differing habitat, beaver harvest, land use patterns, etc.

Minnesota↗