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At least 145 records · Page 8Linked to original sources

Evidence for Paleocene dinosaurs in the Ojo Alamo Sandstone, San Juan Basin, New Mexico

Dinosaur fossils from the Ojo Alamo Sandstone, San Juan Basin, New Mexico, have been a puzzle to geologists for decades because other stratigraphic and paleontologic evidence indicates that this formation is Paleocene in age. The Paleocene age of the Ojo Alamo is based on the following evidence: (1) the lowermost part of the Nacimiento Formation, which conformably overlies and intertongues with the Ojo Alamo Sandstone in the Ojo Alamo type area in the southern part of the basin, contains Paleocene (Puercan) mammalian fossils; (2) plant megafossils collected from the Ojo Alamo indicate a Tertiary age for this rock unit; (3) pollen-productive rock samples collected from the Ojo Alamo at several localities in the basin have, without exception, yielded Paleocene-age palynomorph assemblages, and conversely, no Cretaceous pollen has ever been reported from Ojo Alamo rock samples. We report here the first discovery of Paleocene palynomorphs from Ojo Alamo Sandstone outcrops in the northern San Juan Basin. The pollen-producing rock samples were collected from a coaly, carbonaceous shale bed 12-m above the base of the Ojo Alamo and 3-m below the level of a large hadrosaur femur (1310-mm long and weighing about 130 kg) collected from this site. The bone was recovered from a cobble-to-boulder conglomeratic sandstone but shows no evidence of abrasion by erosion indicating almost instant burial after death of the animal and early mineralization of the femur. It is our opinion that this bone was not reworked from underlying Cretaceous strata. The data from the San Juan River site indicate that dinosaurs did not die out at the end of the Cretaceous in the San Juan Basin, but lived on for as much as a few hundred thousand years into the Paleocene before finally becoming extinct.

New Mexico↗

Spatial mapping of mineralization with manganese-enhanced magnetic resonance imaging

Paramagnetic manganese can be employed as a calcium surrogate to sensitize the magnetic resonance imaging (MRI) technique to the processing of calcium during the bone formation process. At low doses, after just 48h of exposure, osteoblasts take up sufficient quantities of manganese to cause marked reductions in the water proton T1 values compared with untreated cells. After just 24h of exposure, 25??M MnCl2 had no significant effect on cell viability. However, for mineralization studies 100??M MnCl2 was used to avoid issues of manganese depletion in calvarial organ cultures and a post-treatment delay of 48h was implemented to ensure that manganese ions taken up by osteoblasts is deposited as mineral. All specimens were identified by their days in vitro (DIV). Using inductively coupled plasma optical emission spectroscopy (ICP-OES), we confirmed that Mn-treated calvariae continued to deposit mineral in culture and that the mineral composition was similar to that of age-matched controls. Notably there was a significant decrease in the manganese content of DIV18 compared with DIV11 specimens, possibly relating to less manganese sequestration as a result of mineral maturation. More importantly, quantitative T1 maps of Mn-treated calvariae showed localized reductions in T1 values over the calvarial surface, indicative of local variations in the surface manganese content. This result was verified with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). We also found that ??R1 values, calculated by subtracting the relaxation rate of Mn-treated specimens from the relaxation rate of age-matched controls, were proportional to the surface manganese content and thus mineralizing activity. From this analysis, we established that mineralization of DIV4 and DIV11 specimens occurred in all tissue zones, but was reduced for DIV18 specimens because of mineral maturation with less manganese sequestration. In DIV25 specimens, active mineralization was observed for the expanding superficial surface and ??R1 values were increased due to the mineralization of small, previously unmineralized areas. Our findings support the use of manganese-enhanced MRI (MEMRI) to study well-orchestrated mineralizing events that occur during embryonic development. In conclusion, MEMRI is more sensitive to the study of mineralization than traditional imaging approaches. ?? 2011.

Bone↗

Late quaternary regional geoarchaeology of Southeast Alaska Karst: A progress report

Karst systems, sea caves, and rock shelters within the coastal temperate rain forest of Alaska's Alexander Archipelago preserve important records of regional archaeology, sea level history, glacial and climatic history, and vertebrate paleontology. Two 14C AMS dates on human bone discovered in a remote cave (49-PET-408) on Prince of Wales Island document the oldest reliably dated human in Alaska to ca. 9800 B.P. A series of 14C AMS dates from cave deposits span the past 40,000 years and provide the first evidence of Pleistocene faunas from the northwest coast of North America. Other discoveries include sea caves and marine beach deposits elevated above modern sea level, extensive solution caves, and mammalian remains of species previously undocumented within the region. Records of human activity, including cave art, artifacts, and habitation sites may provide new insights into the early human colonization of the Americas. ??1997 John Wiley & Sons, Inc.

Alaska↗

Hind limb malformations in free-living northern leopard frogs (Rana pipiens) from Maine, Minnesota, and Vermont suggest multiple etiologies

Background Reports of malformed frogs have increased throughout the North American continent in recent years. Most of the observed malformations have involved the hind limbs. The goal of this study was to accurately characterize the hind limb malformations in wild frogs as an important step toward understanding the possible etiologies. Methods During 1997 and 1998, 182 recently metamorphosed northern leopard frogs (Rana pipiens) were collected from Minnesota, Vermont, and Maine. Malformed hind limbs were present in 157 (86%) of these frogs, which underwent necropsy and radiographic evaluation at the National Wildlife Health Center. These malformations are described in detail and classified into four major categories: (1) no limb (amelia); (2) multiple limbs or limb elements (polymelia, polydactyly, polyphalangy); (3) reduced limb segments or elements (phocomelia, ectromelia, ectrodactyly, and brachydactyly; and (4) distally complete but malformed limb (bone rotations, bridging, skin webbing, and micromelia). Results Amelia and reduced segments and/or elements were the most common finding. Frogs with bilateral hind limb malformations were not common, and in only eight of these 22 frogs were the malformations symmetrical. Malformations of a given type tended to occur in frogs collected from the same site, but the types of malformations varied widely among all three states, and between study sites within Minnesota. Conclusions Clustering of malformation type suggests that developmental events may produce a variety of phenotypes depending on the timing, sequence, and severity of the environmental insult. Hind limb malformations in free-living frogs transcend current mechanistic explanations of tetrapod limb development.

Maine, Minnesota, Vermont↗

Sublethal effects in avocet and stilt hatchlings from selenium-contaminated sites

Excess selenium (Se) in the aquatic food chain is embryotoxic and teratogenic to avocets, stilts, and other waterbirds. American avocet ( Recurvirostra americana ) and black-necked stilt ( Himantopus mexicanus ) eggs were collected from three sites in the Tulare Lake Basin of California, USA, and hatched in the laboratory. These sites included the Tulare Lake Drainage District-north (TLDD-N, water 2.5 ppb Se), TLDD-south (TLDD-S, water 8.6 ppb Se), and Westfarmers (WF, water 190 ppb Se). Highest egg Se concentrations occurred at WF (geometric mean 31.4 ppm dry wt for avocets and 20.5 ppm dry wt for stilts). Mean egg Se concentrations were 6.7 ppm for avocets and 8.4 ppm for stilts at TLDD-S, and 3.3 ppm for avocets and 2.3 ppm for stilts at TLDD-N. Hatching success and incidence of malformations did not differ among sites, but yolk sac-free hatching weights and bone lengths were less for avocets at the WF site, whereas liver weights and liver to body weight ratios were greater at that site. With increasing Se concentration, oxidative stress was most apparent in avocet hatchlings from WF: hepatic glutathione (GSH) peroxidase activity increased, glucose-6-phosphate dehydrogenase activity decreased, and oxidized glutathione (GSSG) concentration as well as the ratio of GSSG to reduced GSH concentration increased. In stilts, hepatic GSH concentration was lower in WF hatchlings. In conclusion, our findings of Se-impaired embryo growth and hepatotoxicity in avocet hatchlings suggest that oxidative stress observed in hatchlings may be related to these biological effects and may serve as a potential bioindicator of subsequent impaired functions.

California↗

Hormonal control of sulfate uptake by bronchial cartilage of coho salmon: Role of IGF-I

The direct hormonal control of sulfate uptake by cartilage matrix of coho salmon was examined by exposing branchial cartilage to 1 μCi · ml −1 35 SO 4 for 48 hours at 15°C in a defined medium. Sulfate uptake occurred primarily in cartilage (rather than bone) and the amount of specific uptake was similar in epibranchial and ceratobranchial cartilages. Intact and hypophysectomized coho salmon starved for 22 days had equivalent in vitro sulfate uptake, which in both cases were 30% of the uptake seen in branchial cartilage of fed, intact controls. In branchial cartilage from starved coho salmon, in vitro exposure to recombinant bovine insulin-like growth factor I (rbIGF-I) at 1, 10, 100, and 1,000 ng · ml −1 caused a dose-dependent increase in sulfate uptake, with a maximum 3-fold increase over control at 1,000 ng · ml −1 rbIGF-I. Coho salmon insulin (1, 10, 100, and 1,000 ng · ml −1 ) resulted in a maximum 30% increase in sulfate uptake at the highest dose. Growth hormone and triiodo-L-thyronine had no direct effect on in vitro sulfate uptake. The results indicate that IGF-I has direct effects on coho salmon cartilage and may be an important regulator of growth in salmon and other teleosts.

Journal of Experimental Zoology↗

Skeletal morphology of the forefoot in shrews (Mammalia: Soricidae) of the genus Cryptotis, as revealed by digital x-rays

Variation in the forefoot skeleton of small-eared shrews (family Soricidae, genus Cryptotis ) has been previously documented, but the paucity of available skeletons for most taxa makes assessment of the degrees of intraspecific and interspecific variation difficult. We used a digital X-ray system to extract images of the forefoot skeleton from 101 dried skins of eight taxa (seven species, including two subspecies of one species) of these shrews. Lengths and widths of each of the four bones of digit III were measured directly from the digital images, and we used these data to quantify variation within and among taxa. Analysis of the images and measurements showed that interspecific variation exceeds intraspecific variation. In fact, most taxa could be distinguished in multivariate and some bivariate plots. Our quantitative data helped us define a number of specific forefoot characters that we subsequently used to hypothesize evolutionary relationships among the taxa using the exhaustive search option in PAUP, a computer program for phylogenetic analysis. The resulting trees generally concur with previously published evolutionary hypotheses for small-eared shrews. Cryptotis meridensis , a taxon not previously examined in recent phylogenies, is rooted at the base of the branch leading to the C. mexicana group of species. The position of this species suggests that the mostly South American C. thomasi group shares an early ancestor with the C. mexicana group.

Journal of Morphology↗

Can they dig it? Functional morphology and semifossoriality among small-eared shrews, genus Cryptotis (Mammalia, Soricidae)

Small-eared shrews (Mammalia: Soricidae: Cryptotis ), exhibit modifications of the forelimb skeleton that have been interpreted as adaptations for semifossoriality. Most species inhabit remote regions, however, and their locomotory and foraging behaviors remain mostly speculative. To better understand the morphological modifications in the absence of direct observations, we quantified variation in these species by measuring 151 individuals representing 18 species and populations of Cryptotis and two species of moles (Talpidae) for comparison. From our measurements, we calculated 22 indices, most of which have been used previously to characterize substrate use among rodents and other taxa. We analyzed the indices using 1) average percentile ranks, 2) principal components analysis, and 3) cluster analysis. From these analyses, we determined that three basic modes of substrate adaptation are present within Cryptotis : 1) a primarily terrestrial mode, with species that are capable of burrowing, but lack adaptations to increase digging efficiency, 2) a semifossorial mode, with species whose forelimbs bones show strong muscle attachment areas and increased mechanical advantage, and 3) an intermediate mode. In addition to identifying new morphological characters and contributing to our understanding of the functional morphology of soricids, these analyses provide additional insight into the ecology of the species of interest.

Journal of Morphology↗

Fluvial sedimentary history of Arlington Canyon, Channel Islands National Park, California

Arlington Canyon, in the northwest part of Santa Rosa Island, Channel Islands National Park, California, has been the setting for important scientific discoveries over the past half century, including the oldest human remains in North America, several vertebrate fossil sites, and purported evidence of a catastrophic extinction event at the end of the Pleistocene. The canyon is filled with alluvial sediments that date to between 16.4 and 1.1 ka (thousands of calibrated years before present), representing accumulation that occurred primarily in response to rising sea levels during the late Pleistocene and Holocene. The deposits are laterally discontinuous, exhibit a high degree of sedimentary complexity, and contain evidence of past climates and environments, including fossil bones, burned plant macrofossils, and invertebrate microfossils. Here, we show that it is critical to view the observations, data, and conclusions of scientific studies conducted in the canyon within this larger context so that localized facets of the spatially and temporally extensive alluvial deposits are not misinterpreted or misrepresented. By improving the baseline understanding of processes and drivers of sediment accumulation in Arlington Canyon, we hope to offer a solid foundation and better underpinning for future archeological, paleontological, and geochemical studies here and throughout the northern Channel Islands.

California↗

Processing time and precision of aging structures for Bighead Carp and Silver Carp in the lower Red River catchment in the southern Great Plains

Objective Population demographics of invasive species are commonly evaluated to better develop management actions that are useful for reducing their abundance or controlling the population. Bighead Carp Hypophthalmichthys nobilis and Silver Carp H. molitrix are emblematic invaders in the United States, where they continue to expand their range. There is currently no consensus about which hard structure from these species is best for age estimation. Our study objective was to compare the processing time and precision of five hard structures used for age estimation of both species. Methods We sampled fish in the lower Red River catchment of Oklahoma, Arkansas, and Texas during summer and autumn 2021–2022 and removed the lapillus otolith, left primary pectoral fin ray, postcleithrum, urohyal bone, and anterior-most pterygiophore of the dorsal fin from both Bighead and Silver carp. The structures ( n = 1204) were either embedded in epoxy or thin-sectioned and mounted on slides. Two readers estimated the age of the fish by using each structure and came to a consensus. Processing time was recorded from the onset of laboratory processing to the termination of polishing the cross sections for age estimation. Result Processing of otoliths was comparable to or faster than processing of the other structures and resulted in the highest between-reader agreement. The lowest coefficients of variation in age estimation were represented using lapillus otoliths for Bighead Carp and postcleithra for Silver Carp. Our age bias plots indicated that all other structures underestimated age relative to the lapillus otoliths. Conclusion Our results indicated that using lapillus otoliths for age estimation of these species would have the highest between-reader agreement and would incur no additional laboratory processing time. However, validation is needed to assess whether lapillus otoliths correctly age these species. From a management perspective, use of this structure would facilitate improved population comparisons.

Arkansas, Oklahoma, Texas↗

Developmental toxicity of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in nestling American kestrels (Falco sparverius)

Planar PCB congeners are embryotoxic and teratogenic to birds including American kestrels. The developmental toxicity of 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) was studied in the posthatching kestrel as a model for the eagle. Nestlings were dosed orally for 10 days with 5 μl/g body weight of corn oil (controls) or the planar PCB 126 at concentrations of 50, 250, or 1000 ng/g body weight. Dosing with 50 ng/g of PCB 126 resulted in a hepatic concentration of 156 ng/g wet weight, liver enlargement and mild coagulative necrosis, over 10-fold increases in hepatic microsomal ethoxyresorufin- O -dealkylase and benzyloxyresorufin- O -dealkylase, and approximately a 5-fold increase in methoxyresorufin- O -dealkylase. At this dose, mild to moderate lymphoid depletion of the spleen was apparent, as were decreased follicle size and content of the thyroid. At 250 ng/g, concentration of PCB 126 in the liver was 380 ng/g with increasing multifocal coagulative necrosis, decreased bone growth, decreased spleen weight with lymphocyte depletion of the spleen and bursa, and degenerative lesions of the thyroid. At 1000 ng/g, the liver concentration was 1098 ng/g, accompanied by decreased bursa weight, decreased hepatic thiol concentration, and increased plasma enzyme activities (ALT, AST, and LDH-L) in addition to the previous effects. Highly significant positive correlations were noted between liver concentrations of PCB 126 and the ratio of oxidized to reduced glutathone. These findings indicate that nestling kestrels are more susceptible to PCB 126 toxicity than adults, but less sensitive than embryos, and that planar PCBs are of potential hazard to nestling birds..

Fundamental and Applied Toxicology↗

Late Pleistocene Vertebrates and Other Fossils from Epiguruk, Northwestern Alaska

Sediments exposed at Epiguruk, a large cutbank on the Kobuk River about 170 km inland from Kotzebue Sound, record multiple episodes of glacial-age alluviation followed by interstadial downcutting and formation of paleosols. Vertebrate remains from Epiguruk include mammoth, bison, caribou, an equid, a canid, arctic ground squirrel, lemmings, and voles. Radiocarbon ages of bone validated by concordant ages of peat and wood span the interval between about 37,000 and 14,000 yr B.P. The late Pleistocene pollen record is dominated by Cyperaceae, with Artemisia, Salix, Betula, and Gramineae also generally abundant. The fossil record from Epiguruk indicates that the Kobuk River valley supported tundra vegetation with abundant riparian willows during middle and late Wisconsin time. Large herbivores were present during the height of late Wisconsin glaciation as well as during its waning stage and the preceding interstadial interval. The Kobuk River valley would have been a favorable refugium for plants, animals, and possibly humans throughout the last glaciation.

Quaternary Research↗

Late Holocene eolian activity in the mineralogically mature Nebraska Sand Hills

The age of sand dunes in the Nebraska Sand Hills has been controversial, with some investigators suggesting a full-glacial age and others suggesting that they were last active in the late Holocene. New accelerator mass spectrometry radiocarbon ages of unaltered bison bones and organic-rich sediments suggest that eolian sand deposition occurred at least twice in the past 3000 14C yr B.P. in three widely separated localities and as many as three times in the past 800 14C yr at three other localities. These late Holocene episodes of eolian activity are probably the result of droughts more intense than the 1930s "Dust Bowl" period, based on independent Great Plains climate records from lake sediments and tree rings. However, new geochemical data indicate that the Nebraska Sand Hills are mineralogically mature. Eolian sands in Nebraska have lower K-feldspar (and K2O, Rb, and Ba) contents than most possible source sediments and lower K-feldspar contents than dunes of similar age in Colorado. The most likely explanation for mineralogical maturity is reduction of sand-sized K-feldspar to silt-sized particles via ballistic impacts due to strong winds over many cycles of eolian activity. Therefore, dunes of the Nebraska Sand Hills must have had a long history, probably extending over more than one glacial-interglacial cycle, and the potential for reactivation is high, with or without a future greenhouse warming. ?? 1997 University of Washington.

Quaternary Research↗

Small-mammal data on early and middle Holocene climates and biotic communities in the Bonneville Basin, USA

Archaeological investigations in Camels Back Cave, western Utah, recovered a series of small-mammal bone assemblages from stratified deposits dating between ca. 12,000 and 500 14C yr B.P. The cave's early Holocene fauna includes a number of species adapted to montane or mesic habitats containing grasses and/or sagebrush (e.g., Lepus townsendii, Marmota flaviventris, Reithrodontomys megalotis, and Brachylagus idahoensis) which suggest that the region was relatively cool and moist until after 8800 14C yr B.P. Between ca. 8600 and 8100 14C yr B.P. these mammals became locally extinct, taxonomic diversity declined, and there was an increase in species well-adapted to xeric, low-elevation habitats, including ground squirrels, Lepus californicus and Neotoma lepida. The early small-mammal record from Camels Back Cave is similar to the 11,300-6000 14C yr B.P. mammalian sequence from Homestead Cave, northwestern Utah, and provides corroborative data on Bonneville Basin paleoenvironments and mammalian responses to middle Holocene desertification. ?? 2002 University of Washington.

Quaternary Research↗

Environmental contaminants in surrogates, foods, and feathers of California condors (Gymnogyps californianus)

California condor ( Gymnogyps californianus ) foods and feathers, and turkey vultures ( Cathartes aura ), common ravens ( Corvus corax ), and their eggs were collected within the condor range to determine exposure of condors to environmental contaminants. Samples were analyzed for organochlorines and trace elements. Food items contained low concentrations of organochlorines and generally low concentrations of lead. DDE was detected in all vulture carcasses and nearly all raven carcasses at generally moderate concentrations. Other organochlorines occurred infrequently in carcasses and generally at low concentrations. Turkey vulture eggshells were 16% thinner than the pre-DDT mean; there was no change in shell thickness of raven eggs. Vulture eggs contained an average of 6.9 ppm DDE and two contained excessive concentrations of endrin. DDE concentrations were low in raven eggs. Residues of As, Cd, Cr, Cu, Fe, Hg, Ni, Se, Tl, and Zn in tissues of vultures and ravens appeared normal when compared with reference values for other species. Lead concentrations in bone of turkey vultures and feathers of condors appeared to be elevated above normal background concentrations in some cases. Current exposure of condors to organochlorines appears low; however, we are concerned about the excessive exposure of turkey vultures to organochlorines, possibly in Central America. Lead exposure to vultures and condors has occurred, but its significance to their populations is unknown.

Environmental Monitoring and Assessment↗

Lead in hawks, falcons and owls downstream from a mining site on the Coeur D'Alene river, Idaho

Mining and smelting at Kellogg-Smelterville, Idaho, resulted in high concentrations of lead in Coeur d'Alene (CDA) River sediments and the floodplain downstream, where American Kestrels ( Falco sparverius ), Northern Harriers ( Circus cyaneus ), Red-tailed Hawks ( Buteo jamaicensis ), Great Horned Owls ( Bubo virginianus ), and Western Screech-owls ( Otus kennicotti ) nested. Nestling American Kestrels contained significantly higher ( P =0.0012) blood lead concentrations along the CDA River (0.24 µg/g, wet wt) than the nearby reference area (0.087 µg/g). A 35% inhibition of blood δ-aminolevulinic acid dehydratase (ALAD) in nestling Northern Harriers ( P =0.0001), 55% in nestling American Kestrels ( P =0.0001) and 81% in adult American Kestrels ( P =0.0004) provided additional evidence of lead exposure in the CDA River population. In nestling American Kestrels and Northern Harriers, ALAD activity was negatively correlated with lead in blood. An earlier report on Ospreys ( Pandion haliaetus ) showed slightly less inhibition of ALAD than in American Kestrels, but no significant reduction in hemoglobin or hematocrit and no negative influence on production rates. The adult and nestling American Kestrels along the CDA River contained about twice as much blood lead as Ospreys during the same years (adult 0.46 vs. 0.20 µg/g, and nestling 0.24 vs. 0.09 µg/g), but adults showed a 7.5% reduction in hemoglobin ( P =0.0356) and nestlings an 8.2% reduction in hemoglobin ( P =0.0353) and a 5.8% reduction in hematocrit ( P =0.0482). We did not observe raptor deaths related to lead, and although the production rate for American Kestrels was slightly lower along the CDA River, we found no significant negative relation between productivity and lead. Limited data on the other raptors provide evidence of exposure to lead along the CDA River. Several traits of raptors apparently reduce their potential for accumulating critical levels of lead which is primarily stored in bones of prey species.

Environmental Monitoring and Assessment↗

Effects of dietary nickel on survival and growth of mallard ducklings

Mallard ( Anas platyrhynchos ) ducklings were fed nickel sulphate in their diet from day one to 90 days of age. Ducklings fed 1,200 ppm nickel began to tremor and show signs of paresis after 14 days of dosage (age) and 71% of this group died within 60 days of age. Birds fed 1,200 ppm nickel weighed significantly less ( P <0.05) at 28 days of age than birds fed the other diets. Weights of ducklings fed untreated food or dietary dosages of 200 and 800 ppm nickel diets were not significantly different ( P >0.05). The weight/length ratio of the humerus (an expression of bone density) from the 800 ppm diet females was significantly lower ( P <0.05) than the control fed females at 30 and 60 days and for all ducklings fed 1,200 ppm at 30 days of age. The organ-weight/body-weight ratios for heart, liver, and gizzard did not differ from controls or between any dosage group. Liver nickel residues from ducklings that died during this study ranged between 1.0 to 22.7 ppm and kidney residues ranged between 2.7 to 74.4 ppm. Liver and kidney tissues from all ducklings that survived to 90 days of age contained less than 1.0 ppm nickel.

Archives of Environmental Contamination and Toxico↗

Eggshell thickness and reproduction in American kestrels exposed to chronic dietary lead

American kestrels ( Falco sparverius ) were randomly paired and fed 0, 10, or 50 ppm metallic lead in their diet from November 1979–May 1980. Lead levels were elevated in bones and livers of birds receiving the treated diets, particularly the 50 ppm treatment group. Differential deposition of lead was noted between males and females, with the highest levels in the females. No adverse effects were evident with respect to survival, egg laying, or initiation of incubation in any treatment group nor was fertility or eggshell thickness affected. Little or no lead was transferred to the egg contents and although lead was present in the shell, the levels were too variable for this to be considered a useful measure of exposure.

Archives of Environmental Contamination and Toxico↗