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Risk analysis for biological hazards: What we need to know about invasive species

Risk analysis for biological invasions is similar to other types of natural and human hazards. For example, risk analysis for chemical spills requires the evaluation of basic information on where a spill occurs; exposure level and toxicity of the chemical agent; knowledge of the physical processes involved in its rate and direction of spread; and potential impacts to the environment, economy, and human health relative to containment costs. Unlike typical chemical spills, biological invasions can have long lag times from introduction and establishment to successful invasion, they reproduce, and they can spread rapidly by physical and biological processes. We use a risk analysis framework to suggest a general strategy for risk analysis for invasive species and invaded habitats. It requires: (1) problem formation (scoping the problem, defining assessment endpoints); (2) analysis (information on species traits, matching species traits to suitable habitats, estimating exposure, surveys of current distribution and abundance); (3) risk characterization (understanding of data completeness, estimates of the “potential” distribution and abundance; estimates of the potential rate of spread; and probable risks, impacts, and costs); and (4) risk management (containment potential, costs, and opportunity costs; legal mandates and social considerations and information science and technology needs).

Risk Analysis

Suspended-sediment concentration regimes for two biological reference streams in Middle Tennessee

Temporal patterns of suspended-sediment concentration (SSC) duration and frequency (SSC regimes) were characterized and compared with biological impairment thresholds for two headwater streams in the Western Highland Rim of Tennessee. The SSC regimes were plotted as curves showing concentrations and durations of the annual longest and tenth-longest SSC excursions above 18 concentrations for water years 2005-2008 in Copperas Branch and water years 2006 and 2008 in Kelley Creek. Both streams have fish communities remarkably diverse for their small drainage basin areas (420 and 565 ha, respectively), and represent biological reference conditions with respect to SSC. SSC-regime curves were similar for the two sites across water years. The measured SSC regimes reached or exceeded published experimentally based SSC impairment thresholds and plotted below a proposed long-term SSC reference regime for the Interior Plateau ecoregion (Ecoregion 71), suggesting that neither the experimentally based thresholds nor the proposed SSC reference regime adequately reflect the relation between SSC and biological impairment for Western Highland Rim headwater streams. The SSC regimes of the two study streams were similar to the estimated SSC regime of an unimpaired East Tennessee trout stream. Additional field studies are needed to describe SSC regimes in streams of varying basin scale, level of impairment, and region.

Tennessee

Microplastics in Lake Mead National Recreation Area, USA: Occurrence and biological uptake

Microplastics are an environmental contaminant of growing concern, but there is a lack of information about microplastic distribution, persistence, availability, and biological uptake in freshwater systems. This is especially true for large river systems like the Colorado River that spans multiple states through mostly rural and agricultural land use. This study characterized the quantity and morphology of microplastics in different environmental compartments in two large reservoirs along the Colorado River: Lakes Mead and Mohave, within Lake Mead National Recreation Area. To assess microplastic occurrence, surface water and surficial sediment were sampled at a total of nine locations. Sampling locations targeted different sub-basins with varying levels of anthropogenic impact. Las Vegas Wash, a tributary which delivers treated wastewater to Lake Mead, was also sampled. A sediment core (33 cm long, representing approximately 19 years) was extracted from Las Vegas Bay to assess changes in microplastic deposition over time. Striped bass (Morone saxatilis), common carp (Cyprinus carpio), quagga mussels (Dreissena bugensis), and Asian clams (Corbicula fluminea) were sampled at a subset of locations to assess biological uptake of microplastics. Microplastic concentrations were 0.44-9.7 particles/cubic meter at the water surface and 87.5-1,010 particles/kilogram dry weight (kg dw) at the sediment surface. Sediment core concentrations were 220-2,040 particles/kg dw, with no clear increasing or decreasing trend over time. Shellfish microplastic concentrations ranged from 2.7-105 particles/organism, and fish concentrations ranged from 0-19 particles/organism. Fibers were the most abundant particle type found in all sample types. Although sample numbers are small, microplastic concentrations appear to be higher in areas of greater anthropogenic impact. Results from this study improve our understanding of the occurrence and biological uptake of microplastics in Lake Mead National Recreation Area, and help fill existing knowledge gaps on microplastics in freshwater environments in the southwestern U.S.

Nevada

[Book Review] Biology of marine birds

A text devoted to the biology and ecology of marine birds has not been published in the last 15 years. Although a number of more taxa-specific texts have been produced during that period, there has not been a single publication that attempted to review our knowledge of all the major seabird orders since the works of Nelson (1979), Croxall (1987), and Furness and Monaghan (1987). Following the publication of those works, a large and impressive body of literature has been produced. Given the rapid expansion of the field in the last two decades, the time was ripe for production of an extensive compendium on the biology, ecology, and conservation of the world's seabirds. E. A. Schreiber and J. Burger are editors of this CRC publication, Biology of Marine Birds . The book consists of 19 chapters that vary in length from 15 to 51 pages. There are also two extensive appendices: (1) a list of seabird species (restricted to the orders Sphenisciformes, Procellariiformes, Pelecaniformes, and Charadriiformes, the latter limited to Stercorariidae, Laridae, Rhynchopidae, and Alcidae) and their IUCN status, and (2) a very useful table of species-specific life-history traits. The 19 chapters were prepared by 26 authors, among them some of the most respected and published seabird scientists in the world. A brief preface introduces the book, its objective (to provide an examination and summary of the research on seabirds), its audience (researchers, conservationists, managers, and policy-makers), and the taxa covered. The editors coauthored the introductory chapter, Seabirds in the Marine Environment. The authors describe distinctive characteristics of seabird life-histories in comparison to other taxa, hypotheses for why those lifestyles evolved and the potential role of energy limitation in the evolution of seabird life-histories. Along with a discussion of other common seabird traits, such as a propensity for colonial breeding, the authors also suggest directions for future research in seabird ecology.

The Auk

Breeding biology of an afrotropical forest understory bird community in northeastern Tanzania

Many aspects of the breeding biology of Afrotropical forest birds are poorly known. Here we provide a description based on the monitoring of 1461 active nests over eight breeding seasons about one or more aspects of the breeding biology for 28 coexisting understory bird species on the Amani Plateau in the East Usambara Mountains, Tanzania. Mean nest height and mean distance of nest from forest edge varied widely among species with most species constructing nests across a broad vertical and forest edge to interior gradient. However, there were important exceptions with all sunbird species and several dove and waxbill species constructing nests in close proximity to the forest edge. For 17 common species for which we recorded two or more active nests, mean clutch size across species was 1.9 eggs per clutch, the lowest site-specific mean clutch size yet reported for a tropical forest bird community. For nine bird species, a subset of the 17 common species, length of breeding season, defined as the difference between the earliest and latest recorded incubation onset date, ranged from 88–139 days. Most of these nine species displayed a unimodal distribution in incubation onset dates across a breeding season which extended from the end of August through middle January. In summary, a wide variation exists in most aspects of the breeding biology within an understory forest bird community in the East Usambara Mountains.

Wilson Journal of Ornithology

Untangling the biological contributions to soil stability in semiarid shrublands

Communities of plants, biological soil crusts (BSCs), and arbuscular mycorrhizal (AM) fungi are known to influence soil stability individually, but their relative contributions, interactions, and combined effects are not well understood, particularly in arid and semiarid ecosystems. In a landscape-scale field study we quantified plant, BSC, and AM fungal communities at 216 locations along a gradient of soil stability levels in southern Utah, USA. We used multivariate modeling to examine the relative influences of plants, BSCs, and AM fungi on surface and subsurface stability in a semiarid shrubland landscape. Models were found to be congruent with the data and explained 35% of the variation in surface stability and 54% of the variation in subsurface stability. The results support several tentative conclusions. While BSCs, plants, and AM fungi all contribute to surface stability, only plants and AM fungi contribute to subsurface stability. In both surface and subsurface models, the strongest contributions to soil stability are made by biological components of the system. Biological soil crust cover was found to have the strongest direct effect on surface soil stability (0.60; controlling for other factors). Surprisingly, AM fungi appeared to influence surface soil stability (0.37), even though they are not generally considered to exist in the top few millimeters of the soil. In the subsurface model, plant cover appeared to have the strongest direct influence on soil stability (0.42); in both models, results indicate that plant cover influences soil stability both directly (controlling for other factors) and indirectly through influences on other organisms. Soil organic matter was not found to have a direct contribution to surface or subsurface stability in this system. The relative influence of AM fungi on soil stability in these semiarid shrublands was similar to that reported for a mesic tallgrass prairie. Estimates of effects that BSCs, plants, and AM fungi have on soil stability in these models are used to suggest the relative amounts of resources that erosion control practitioners should devote to promoting these communities. This study highlights the need for system approaches in combating erosion, soil degradation, and arid-land desertification.

Utah

On the use of log-transformation vs. nonlinear regression for analyzing biological power laws

Power‐law relationships are among the most well‐studied functional relationships in biology. Recently the common practice of fitting power laws using linear regression (LR) on log‐transformed data has been criticized, calling into question the conclusions of hundreds of studies. It has been suggested that nonlinear regression (NLR) is preferable, but no rigorous comparison of these two methods has been conducted. Using Monte Carlo simulations, we demonstrate that the error distribution determines which method performs better, with NLR better characterizing data with additive, homoscedastic, normal error and LR better characterizing data with multiplicative, heteroscedastic, lognormal error. Analysis of 471 biological power laws shows that both forms of error occur in nature. While previous analyses based on log‐transformation appear to be generally valid, future analyses should choose methods based on a combination of biological plausibility and analysis of the error distribution. We provide detailed guidelines and associated computer code for doing so, including a model averaging approach for cases where the error structure is uncertain.

Ecology

Monitoring biological diversity: strategies, tools, limitations, and challenges

Monitoring is an assessment of the spatial and temporal variability in one or more ecosystem properties, and is an essential component of adaptive management. Monitoring can help determine whether mandated environmental standards are being met and can provide an early-warning system of ecological change. Development of a strategy for monitoring biological diversity will likely be most successful when based upon clearly articulated goals and objectives and may be enhanced by including several key steps in the process. Ideally, monitoring of biological diversity will measure not only composition, but also structure and function at the spatial and temporal scales of interest. Although biodiversity monitoring has several key limitations as well as numerous theoretical and practical challenges, many tools and strategies are available to address or overcome such challenges; I summarize several of these. Due to the diversity of spatio-temporal scales and comprehensiveness encompassed by existing definitions of biological diversity, an effective monitoring design will reflect the desired sampling domain of interest and its key stressors, available funding, legal requirements, and organizational goals.

Northwestern Naturalist

Strategic plan for the U.S. Geological Survey status and trends of Biological Resources Program: 2004-2009

The mission of the USGS Status and Trends of Biological Resources Program is to measure, predict, assess, and report the status and trends of the Nation's biological resources to facilitate research, enable resource management and stewardship, and promote public understanding and appreciation of our living resources. Determining the status (abundance, distribution, productivity, and health) and trends (how these variables change over time) of our living natural resources is critical for their Trumpeter swan with numbered wing tags. This tech- protection or restoration. The Progg ran nique allows birds to he monitored remotely without the provides the USGS, other agencies of need for recapture to identify individuals. Photo by the Department of the Interior (DOI), Wayne Miller. other federal and state agencies, and the public with science-based monitoring data and information for local, regional, and national assessment of biological resources and the ecosystems that support them.

Circular

Endocrine active chemicals, pharmaceuticals, and other chemicals of concern in surface water, wastewater-treatment plant effluent, and bed sediment, and biological characteristics in selected streams, Minnesota: Design, methods, and data, 2009

This report presents the study design, environmental data, and quality-assurance data for an integrated chemical and biological study of selected streams or lakes that receive wastewater-treatment plant effluent in Minnesota. This study was a cooperative effort of the U.S. Geological Survey, the Minnesota Pollution Control Agency, St. Cloud State University, the University of St. Thomas, and the University of Colorado. The objective of the study was to identify distribution patterns of endocrine active chemicals, pharmaceuticals, and other organic and inorganic chemicals of concern indicative of wastewater effluent, and to identify biological characteristics of estrogenicity and fish responses in the same streams. The U.S. Geological Survey collected and analyzed water, bed-sediment, and quality-assurance samples, and measured or recorded streamflow once at each sampling location from September through November 2009. Sampling locations included surface water and wastewater-treatment plant effluent. Twenty-five wastewater-treatment plants were selected to include continuous flow and periodic release facilities with differing processing steps (activated sludge or trickling filters) and plant design flows ranging from 0.002 to 10.9 cubic meters per second (0.04 to 251 million gallons per day) throughout Minnesota in varying land-use settings. Water samples were collected from the treated effluent of the 25 wastewater-treatment plants and at one point upstream from and one point downstream from wastewater-treatment plant effluent discharges. Bed-sediment samples also were collected at each of the stream or lake locations. Water samples were analyzed for major ions, nutrients, trace elements, pharmaceuticals, phytoestrogens and pharmaceuticals, alkylphenols and other neutral organic chemicals, carboxylic acids, and steroidal hormones. A subset (25 samples) of the bed-sediment samples were analyzed for carbon, wastewater-indicator chemicals, and steroidal hormones; the remaining samples were archived. Biological characteristics were determined by using an in-vitro bioassay to determine total estrogenicity in water samples and a caged fish study to determine characteristics of fish from experiments that exposed fish to wastewater effluent in 2009. St. Cloud State University deployed and processed caged fathead minnows at 13 stream sites during September 2009 for the caged fish study. Measured fish data included length, weight, body condition factor, and vitellogenin concentrations.

Minnesota

Hydrologic, sediment, and biological data associated with irrigation drainage in the middle Green River basin, Utah and Colorado, water years 1991-2000

Hydrologic, sediment, and biological data were collected in the middle Green River basin in eastern Utah from 1991 to 2000 in an effort to monitor the effects of irrigation drainage on wetland areas and streams, aid in the development of remediation plans, and evaluate the effectiveness of selenium remediation efforts at Stewart Lake Waterfowl Management Area (WMA). Data consist primarily of selenium concentrations in surface water, ground water, bottom sediment, and biological samples. Supporting hydrologic data include field measurements of temperature, pH, specific conductance, water levels in wells, and discharge at surface-water sites. Selected water samples also were analyzed for major ions, trace elements, nutrients, and gross alpha and beta radiation. The concentration of selected selenium species is reported for several bottom-sediment samples from Stewart Lake WMA and the concentration of total selenium in suspended-sediment samples from the area are included. Well logs for six wells installed at Stewart Lake WMA are presented along with trace-element data for several biological samples collected at selected sites throughout the middle Green River basin.

Colorado, Utah, Wyoming

Determination of total mercury in biological and geological samples

The analytical chemist is faced with several challenges when determining mercury in biological and geological materials. These challenges include widespread mercury contamination, both in the laboratory and the environment, possible losses of mercury during sample preparation and digestion, the wide range of mercury values commonly observed, ranging from the low nanogram per gram or per liter for background areas to hundreds of milligrams per kilogram in contaminated or ore-bearing areas, great matrix diversity, and sample heterogeneity1. These factors can be naturally occurring or anthropogenic, but must be addressed to provide a precise and accurate analysis. Although there are many instrumental methods available for the successful determination of mercury, no one technique will address all problems or all samples all of the time. The approach for the determination of mercury used at the U.S. Geological Survey, Crustal Imaging and Characterization Team, Denver Laboratories, utilizes a suite of complementary instrumental methods when approaching a study requiring mercury analyses. Typically, a study could require the analysis of waters, leachates or selective digestions of solids, vegetation, and biological materials such as tissue, bone, or shell, soils, rocks, sediments, coals, sludges, and(or) ashes. No one digestion or sample preparation method will be suitable for all of these matrices. The digestions typically employed at our laboratories include: (i) a closed-vessel microwave method using nitric acid and hydrogen peroxide, followed by digestion/dilution with a nitric acid/sodium dichromate solution, (ii) a robotic open test-tube digestion with nitric acid and sodium dichromate, (iii) a sealed Teflon? vessel with nitric acid and sodium dichromate, (iv) a sealed glass bottle with nitric acid and sodium dichromate, or (v) open test tube digestion with nitric and sulfuric acids and vanadium pentoxide. The common factor in all these digestions is that they are very oxidative to ensure the conversion of all mercury forms into Hg (II). Each method of digestion has its advantages and limitations. The method of detection used in our laboratories involves a combination of an in-house, custom, classic continuous-flow cold-vapor atomic absorption spectrometry (CVAAS), a commercially available, automated, flow-injection and a continuous flow cold-vapor atomic fluorescence spectrometry (CV-AFS) systems, and a relatively new, automated and integrated approach where solid or liquid samples are thermally decomposed under an oxygen atmosphere (a nitrogen atmosphere is used for coals) and the released mercury vapor trapped onto a gold gauze and then thermally released into an AAS system. Other less frequently used instrumental methods available for the determination of mercury include inductively coupled plasma ? optical emission spectrometry (ICP-OES), inductively couple plasma ? mass spectrometry (ICP-MS) (both solution nebulization and laser ablation), and instrumental neutron activation analysis (INAA). Results from two case studies involving the determination of mercury in the challenging matrices of biological materials will be presented. These will include fillet, liver and stomach-content samples from grayling for a baseline/background study in Alaska, and samples of meat tissue and shell material from Tanner crabs from Glacier Bay, Alaska. These studies show that the method of digestion is more important than a very sensitive detection limit for mercury.

Open-File Report

Water-quality, bed-sediment, and biological data (October 2003 through September 2004) and statistical summaries of data for streams in the Upper Clark Fork basin, Montana

Water, bed sediment, and biota were sampled in streams from Butte to below Missoula as part of a program, conducted in cooperation with the U.S. Environmental Protection Agency, to characterize aquatic resources in the upper Clark Fork basin of western Montana. Sampling sites were located on the Clark Fork, five major tributaries, and three smaller tributaries. Water-quality samples were collected periodically at 17 sites during October 2003 through September 2004 (water year 2004). Bed-sediment and biological samples were collected one time in August 2004. The primary constituents analyzed were trace elements associated with tailings from historical mining and smelting activities. This report summarizes the results of water-quality, bed-sediment, and biota samples collected in water year 2004 and provides statistical summaries of data collected since 1985. Water-quality data for samples collected periodically from streams include concentrations of selected major ions, trace elements, and suspended sediment. Daily values of suspended-sediment concentration and suspended-sediment discharge were determined for three sites. Bed-sediment data include trace-element concentrations in the fine-grained and bulk fractions. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Quality-assurance data are reported for analytical results of water, bed sediment, and biota. Statistical summaries of water-quality, bed-sediment, and biological data are provided for the period of record since 1985 for each site.

Montana

Water-quality, bed-sediment, and biological data (October 2006 through September 2007) and statistical summaries of long-term data for streams in the Clark Fork Basin, Montana

Water, bed sediment, and biota were sampled in streams from Butte to below Milltown Reservoir as part of a long-term monitoring program in the upper Clark Fork basin; additional water-quality samples were collected in the Clark Fork basin from sites near Milltown Reservoir downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water-quality samples were collected periodically at 22 sites from October 2006 through September 2007. Bed-sediment and biological samples were collected once at 12 sites during August 2007. This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2006 through September 2007. Water-quality data include concentrations of selected major ions, trace elements, and suspended sediment. Turbidity was analyzed for samples collected at sites where seasonal daily values of turbidity were being determined. Nutrients also were analyzed in the supplemental water-quality samples. Daily values of suspended-sediment concentration and suspended-sediment discharge were determined for four sites, and seasonal daily values of turbidity were determined for five sites. Bed-sediment data include trace-element concentrations in the fine-grained fraction. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record since 1985.

Montana

Design tradeoffs for trend assessment in aquatic biological monitoring programs

Assessments of long-term (multiyear) temporal trends in biological monitoring programs are generally undertaken without an adequate understanding of the temporal variability of biological communities. When the sources and levels of variability are unknown, managers cannot make informed choices in sampling design to achieve monitoring goals in a cost-effective manner. We evaluated different trend sampling designs by estimating components of both short- and long-term variability in biological indicators of water quality in streams. Invertebrate samples were collected from 32 sites—9 urban, 6 agricultural, and 17 relatively undisturbed (reference) streams—distributed throughout the United States. Between 5 and 12 yearly samples were collected at each site during the period 1993–2008, plus 2 samples within a 10-week index period during either 2007 or 2008. These data allowed calculation of four sources of variance for invertebrate indicators: among sites, among years within sites, interaction among sites and years (site-specific annual variation), and among samples collected within an index period at a site (residual). When estimates of these variance components are known, changes to sampling design can be made to improve trend detection. Design modifications that result in the ability to detect the smallest trend with the fewest samples are, from most to least effective: (1) increasing the number of years in the sampling period (duration of the monitoring program), (2) decreasing the interval between samples, and (3) increasing the number of repeat-visit samples per year (within an index period). This order of improvement in trend detection, which achieves the greatest gain for the fewest samples, is the same whether trends are assessed at an individual site or an average trend of multiple sites. In multiple-site surveys, increasing the number of sites has an effect similar to that of decreasing the sampling interval; the benefit of adding sites is greater when a new set of different sites is selected for each sampling effort than when the same sites are sampled each time. Understanding variance components of the ecological attributes of interest can lead to more cost-effective monitoring designs to detect trends.

Alabama;Arizona;Arkansas;California;Colorado;Georg

Report of the Interagency biological methods workshop

The U.S. Geological Survey hosted the Interagency Biological Methods Workshop in Reston, Virginia, during June 22-23, 1993. The purposes of the workshop were to (1) promote better communication among Federal agencies that are using or developing biological methods in water-quality assessment programs for streams and rivers, and (2) facilitate the sharing of data and interagency collaboration. The workshop was attended by 45 biologists representing numerous Federal agencies and programs, and a few regional and State programs that were selected to provide additional perspectives. The focus of the workshop was community assessment methods for fish, invertebrates, and algae; physical habitat characterization; and chemical analyses of biological tissues. Charts comparing program objectives, design features, and sampling methods were compiled from materials that were provided by participating agencies prior to the workshop and formed the basis for small workgroup discussions. Participants noted that differences in methods among programs were often necessitated by differences in program objectives. However, participants agreed that where programs have identified similar data needs, the use of common methods is beneficial. Opportunities discussed for improving data compatibility and information sharing included (1) modifying existing methods, (2) adding parameters, (3) improving access to data through shared databases (potentially with common database structures), and (4) future collaborative efforts that range from research on selected protocol questions to followup meetings and continued discussions.

Open-File Report

Biology as an integrated component of the U.S. Geological Survey's National Water-Quality Assessment Program

The U.S. Geological Survey?s (USGS) National Water-Quality Assessment (NAWQA) Program is designed to integrate chemical, physical, and biological data to assess the status of and trends in the Nation?s water quality at local, regional, and national levels. The Program consists of 60 study units (major river basins and large parts of aquifers) located throughout the Nation (fig. 1). Data are collected at stream, river, and ground-water sites that represent the Nation?s mix of major natural and human factors that influence water quality. Biological data are collected from streams and rivers, and include (1) fish and other aquatic organisms whose tissues are analyzed for a wide array of chemical contaminants; (2) characterizations of algal, benthic invertebrate, and fish communities; and (3) characterizations of vegetation growing in streams and along streambanks. These biological data are collected in conjunction with physical (streamflow, characterizations of instream, bank, and flood-plain habitats) and chemical data.

Open-File Report