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At least 127 records · Page 7Linked to original sources

Survival, growth and reproduction of non-indigenous Nile tilapia, Oreochromis niloticus (Linnaeus 1758). I. Physiological capabilities in various temperatures and salinities

The physiological tolerances of non-native fishes is an integral component of assessing potential invasive risk. Salinity and temperature are environmental variables that limit the spread of many non-native fishes. We hypothesised that combinations of temperature and salinity will interact to affect survival, growth, and reproduction of Nile tilapia, Oreochromis niloticus , introduced into Mississippi, USA. Tilapia withstood acute transfer from fresh water up to a salinity of 20 and survived gradual transfer up to 60 at typical summertime (30°C) temperatures. However, cold temperature (14°C) reduced survival of fish in saline waters ≥10 and increased the incidence of disease in freshwater controls. Although fish were able to equilibrate to saline waters in warm temperatures, reproductive parameters were reduced at salinities ≥30. These integrated responses suggest that Nile tilapia can invade coastal areas beyond their point of introduction. However, successful invasion is subject to two caveats: (1) wintertime survival depends on finding thermal refugia, and (2) reproduction is hampered in regions where salinities are ≥30. These data are vital to predicting the invasion of non-native fishes into coastal watersheds. This is particularly important given the predicted changes in coastal landscapes due to global climate change and sea-level rise.

Mississippi

Role of prolactin, growth hormone, insulin-like growth factor I and cortisol in Teleost Osmoregulation

This chapter focuses on the endocrine mechanisms that control the overall capacity of the ion regulatory mechanisms in teleost fish, focusing on the osmoregulatory actions of prolactin (PRL), the growth hormone (GH)/insulin-like growth factor I (IGF-I) axis and cortisol. PRL has been shown to regulate several aspects of the ion regulatory mechanisms that are characteristic of freshwater (FW) fish. In FW, teleosts must counteract the passive loss of ions and gain of water by actively taking up ions, and removing excess water by excreting a dilute urine. Cortisol is the major corticosteroid produced by the interrenal tissue of teleost fish. This hormone has several established physiological roles related to osmoregulation, intermediary metabolism, growth, stress and immune function. In addition to the independent osmoregulatory actions of PRL, GH/IGF-I axis and cortisol, there is substantial evidence indicating the existence of synergy and antagonism of these hormones with one another.

Book chapter

Osmoregulatory actions of the GH/IGF axis in non-salmonid teleosts

Salmonid fishes provided the first findings on the influence of the growth hormone (GH)/insulin-like growth factor I (IGF-I) axis on osmoregulation in teleost fishes. Recent studies on non-salmonid species, however, indicate that this physiological action of the GH/IGF-I axis is not restricted to salmonids or anadromous fishes. GH-producing cells in the pituitary of fish acclimated to different salinities show different degrees of activation depending on the species studied. Plasma GH levels either increase or do not change after transfer of fish from freshwater to seawater. Treatment with GH or IGF-I increases salinity tolerance and/or increases gill Na + ,K + -ATPase activity of killifish ( Fundulus heteroclitus ), tilapia ( Oreochromis mossambicus and Oreochromis niloticus ) and striped bass ( Morone saxatilis ). As in salmonids, a positive interaction between GH and cortisol for improving hypoosmoregulatory capacity has been described in tilapia ( O . mossambicus ). Research on the osmoregulatory role of the GH/IGF-I axis is derived from a small number of teleost species. The study of more species with different osmoregulary patterns will be necessary to fully clarify the osmoregulatory role of GH/IGF-I axis in fish. The available data does suggest, however, that the influence of the GH/IGF-I axis on osmoregulation may be a common feature of euryhalinity in teleosts.

Comparative Biochemistry and Physiology, Part B: B

The future of fish passage science, engineering, and practice

Much effort has been devoted to developing, constructing and refining fish passage facilities to enable target species to pass barriers on fluvial systems, and yet, fishway science, engineering and practice remain imperfect. In this review, 17 experts from different fish passage research fields (i.e., biology, ecology, physiology, ecohydraulics, engineering) and from different continents (i.e., North and South America, Europe, Africa, Australia) identified knowledge gaps and provided a roadmap for research priorities and technical developments. Once dominated by an engineering‐focused approach, fishway science today involves a wide range of disciplines from fish behaviour to socioeconomics to complex modelling of passage prioritization options in river networks. River barrier impacts on fish migration and dispersal are currently better understood than historically, but basic ecological knowledge underpinning the need for effective fish passage in many regions of the world, including in biodiversity hotspots (e.g., equatorial Africa, South‐East Asia), remains largely unknown. Designing efficient fishways, with minimal passage delay and post‐passage impacts, requires adaptive management and continued innovation. While the use of fishways in river restoration demands a transition towards fish passage at the community scale, advances in selective fishways are also needed to manage invasive fish colonization. Because of the erroneous view in some literature and communities of practice that fish passage is largely a proven technology, improved international collaboration, information sharing, method standardization and multidisciplinary training are needed. Further development of regional expertise is needed in South America, Asia and Africa where hydropower dams are currently being planned and constructed.

Fish and Fisheries

Osmoregulatory actions of growth hormone and its mode of action in salmonids: A review

Osmoregulatory actions of growth hormone (GH) and its mode of action in salmonids are reviewed. We present evidence suggesting that insulin-like growth factor I (IGF-I) mediates some of the actions of GH on seawater acclimation. Plasma concentration and turnover of GH rise following exposure to seawater. Exogenous GH ( in vivo ) increases gill Na + ,K + -ATPase activity and the number of gill chloride cells, and inhibits an increase in plasma osmolarity and ions following transfer of fish to seawater. A single class of high affinity GH receptors is present in the liver, gill, intestine, and kidney. The levels of IGF-I mRNA in the liver, gill and kidney increased after GH-injection. After transfer to seawater, IGF-I mRNA increased in the gill and kidney following the rise in plasma GH, although no significant change was seen in the liver. Injection of IGF-I improved the ability of the fish to maintain plasma sodium levels after transfer to seawater. GH treatment also sensitizes the interrenal to adrenocorticotropin (ACTH), increasing cortisol secretion. Both cortisol and IGF-I may be involved in mediating the action of GH in seawater adaptation, although studies on the effect of GH on osmoregulatory physiology of non-salmonid species are limited. An integrated model of the osmoregulatory actions of GH is presented, and areas in need of research are outlined.

Fish Physiology and Biochemistry

Relative-condition parameters for fishes of Montana, USA

Body condition indices are commonly used in the management of fish populations and are a surrogate to physiological attributes such as tissue-energy reserves. Relative condition factor ( K n ) describes the condition of species relative to populations in a geographic area. We developed models to allow for the calculation of K n in Montana, USA by using the weight–length data collected by Montana Fish, Wildlife & Parks. We generated log 10 weight–log 10 length relationships to obtain Montana specific parameter estimates for relative condition equations ( W′ ) for 51 species and three subspecies. We developed separate models by water type (e.g., lotic and lentic) and sex for five species due to varying growth based on sexual dimorphism and varying ecosystem types. Relative condition offers the advantage of describing body condition relative to species in Montana, provides a condition index for species that do not have standard-weight models developed for relative weight ( Wr ), and affords more information for the global database on weight–length relationships of fishes.

Montana

Physiological response of juvenile coho salmon (Oncorhynchus kisutch) and rainbow trout (Salmo gairdneri) to handling and crowding stress in intensive fish culture

Moving 4–5-in. coho salmon ( Oncorhynchus kisutch ) held in soft (20 ppm CaCO 3 ) water from the relatively light loading density of 0.5 lb/ft 3 to 1, 2, or 4 lb/ft 3 (density index, DI = 0.1, 0.2, 0.4, 0.8) caused significant stress as indicated by loss of feeding behavior, but only minimal physiological disturbances, as indicated by lack of hyperglycemia or hypochloremia. However, moving them to 6 or 12 lb/ft 3 (DI = 1.2, 2.4) caused significant physiological stress which required at least a week for recovery. Smolting coho salmon were physiologically stressed by population densities of 1 lb/ft 3 or more and a subclinical corynebacterial kidney infection was activated. Rainbow trout ( Salmo gairdneri ) (4–5 in.) were physiologically stressed when moved and held at 1 lb/ft 3 or more but retained normal feeding behavior. This indicates that handling and crowding stress will be minimized in softwater areas if densities in fish distribution trucks or in ponds or raceways during disease treatments are held to 0.1–0.5 lb/gal.

Journal of the Fisheries Research Board of Canada

Effects of acute thermal stress on the survival, predator avoidance, and physiology of juvenile fall Chinook salmon

We subjected juvenile fall chinook salmon from the Hanford Reach of the Columbia River to acute thermal stressors in the laboratory that were derived from field data. We assessed the effects of thermal stress on: (1) the extent of direct mortality; (2) the vulnerability of fish to predation by smallmouth bass; and (3) some general physiological stress responses and synthesis of heat shock protein 70 (hsp70). Thermally-stressed fish showed little direct mortality and no increases in vulnerability to predation. However, these fish showed transient increases in plasma concentrations of cortisol, glucose, and lactate, and a dramatic (25-fold higher than controls) and persistent (lasting 2 wk) increase in levels of liver hsp70. Our results indicate that exposure of Hanford Reach juvenile fall chinook salmon to such stressors did not lead to significant increases in direct mortality or vulnerability to predation, but did alter physiological homeostasis, which should be of concern to those managing this resource. Because our fish received only a single exposure to one of the stressors we examined, we are also concerned about the consequences of exposing fish to multiple, cumulative stressors - a likely scenario for fish in the wild.

Northwest Science

Physiological indices of seawater readiness in postspawning steelhead kelts

Management goals to improve the recovery of steelhead (Oncorhynchus mykiss) stocks at risk of extinction include increasing the proportion of postspawning fish that survive and spawn again. To be successful, postspawning steelhead (kelts) migrating downstream to the ocean must prepare physiologically and physically for a seawater transition. We sampled blood, gill filaments, and evaluated the external condition of migrating kelts from an ESA-listed population in the Snake/Columbia River system over two consecutive years to evaluate their physiological readiness for transition to seawater. We chose attributes often considered as measures of preparation for seawater in juveniles, including gill Na + ,K + ATPase activity, plasma electrolytes and hormones to consider factors related to external condition, size and sex. We found kelts in good external condition had plasma profiles similar to downstream-migrating smolts. In addition, we found more than 80% of kelts ranked in good external condition had smolt-like body silvering. We compared measures from migrating kelts with samples obtained from hatchery fish at the time of spawning to confirm that Na + , K + ATPase activity in kelts was significantly elevated over spawning fish. We found significant differences in gill Na + , K + ATPase activity in migrating kelts between the years of sampling, but little indication of influence of fish condition. We conclude that the postspawning steelhead sampled exhibited a suite of behaviours, condition and physiology characteristic of fish prepared for successful transition to a seawater environment.

Idaho;Washington

Ecophysiology of two mesophotic octocorals intended for restoration: Effects of light and temperature

Light and temperature are driving forces that shape the evolution and physiology of mesophotic organisms. On the Mississippi-Alabama continental shelf, octocorals dominate the mesophotic seascape and provide habitat for many fish and invertebrate species. Gaps in knowledge regarding the fundamental physiological responses of these species to light and temperature are of particular interest to restoration activities following the Deepwater Horizon oil spill. To address these gaps, the photobiology and thermal tolerance of Swiftia exserta and Muricea pendula were assessed in the field and laboratory. Pulse amplitude modulated fluorometry, histology, light microscopy, and epifluorescence imaging revealed low densities of photosynthetic endobionts in samples of S. exserta and none in samples of M. pendula collected near the determined bottom of the euphotic zone (51.45 m). Response to the recorded monthly mean habitat temperature range (18.5–25.4°C) was assessed using respirometry and polyp activity data from live corals exposed to temperatures between 18°C and 26°C. There was no significant difference in oxygen consumption for either species between 18°C and 26°C, and calculated Q 10 values were not significantly different from 1, thus suggesting that both species have a low sensitivity to the local thermal environment. However, a negative correlation between temperature and polyp activity suggests that M. pendula is more sensitive to higher temperatures than S. exserta . This study improves the understanding of the effects of light and temperature on mesophotic octocoral physiology and lays the foundation for future work to explore the thermal thresholds of each species and the endobiont–host relationship in S. exserta .

Alabama, Mississippi, South Carolina

Sublethal effects of catch-and-release fishing: measuring capture stress, fish impairment, and predation risk using a condition index

The sublethal effects of simulated capture of red snapper ( Lutjanus campechanus ) were analysed using physiological responses, condition indexing, and performance variables. Simulated catch-and-release fishing included combinations of depth of capture and thermocline exposure reflective of environmental conditions experienced in the Gulf of Mexico. Frequency of occurrence of barotrauma and lack of reflex response exhibited considerable individual variation. When combined into a single condition or impairment index, individual variation was reduced, and impairment showed significant increases as depth increased and with the addition of thermocline exposure. Performance variables, such as burst swimming speed (BSS) and simulated predator approach distance (AD), were also significantly different by depth. BSSs and predator ADs decreased with increasing depth, were lowest immediately after release, and were affected for up to 15 min, with longer recovery times required as depth increased. The impairment score developed was positively correlated with cortisol concentration and negatively correlated with both BSS and simulated predator AD. The impairment index proved to be an efficient method to estimate the overall impairment of red snapper in the laboratory simulations of capture and shows promise for use in field conditions, to estimate release mortality and vulnerability to predation.

ICES Journal of Marine Science

Physiological effects of handling and hauling stress on smallmouth bass

Mortalities associated with the handling and transporting (hauling) of fishes have long been a problem. Tolerance to these stressors varies greatly among species (Davis and Parker 1979; Tomasso et al. 1980; Wydoski and Wedenmeyer 1976). In most fishes, however, handling and hauling losses are caused by two major factors - activation of latent infections as a result of increased endocrine activity (cf. Wedemeyer 1970), and osmoregulatory dysfunctions due to blood electrolyte disturbances (Lewis 1971; Wydoski and Wedemeyer 1976). Basic physiological information on the stress caused by current hatchery practices is helpful in developing new and improved techniques to increase survival. In view of the present fishery management requirements for stocking smallmouth bas ( Micropterus dolomieu ), baseline information on the physiological effects of handling and hauling hatchery-reared fish is needed to serve as the foundation for improving transport methods. Shell (1959) summarized several physiological characteristics of smallmouth bass, but little information on their physiological tolerance to stress exists. The present study was designed to determine the physiological effects of handling and short-term hauling in small mouth bass. Plasma chloride, sodium, potassium, and glucose dynamics were monitored in indicate the severity of the resulting stress and the recovery time needed.

Progressive Fish-Culturist

Lipid and moisture content modeling of amphidromous Dolly Varden using bioelectrical impedance analysis

The physiological well-being or condition of fish is most commonly estimated from aspects of individual morphology. However, these metrics may be only weakly correlated with nutritional reserves stored as lipid, the primary form of accumulated energy in fish. We constructed and evaluated bioelectrical impedance analysis (BIA) models as an alternative method of assessing condition in amphidromous Dolly Varden Salvelinus malma collected from nearshore estuarine and lotic habitats of the Alaskan Arctic. Data on electrical resistance and reactance were collected from the lateral and ventral surfaces of 192 fish, and whole-body percent lipid and moisture content were determined using standard laboratory methods. Significant inverse relationships between temperature and resistance and reactance prompted the standardization of these data to a constant temperature using corrective equations developed herein. No significant differences in resistance or reactance were detected among spawning and nonspawning females after accounting for covariates, suggesting that electrical pathways do not intersect the gonads. Best-fit BIA models incorporating electrical variables calculated from the lateral and ventral surfaces produced the strongest associations between observed and model-predicted estimates of proximate content. These models explained between 6% and 20% more of the variability in laboratory-derived estimates of proximate content than models developed from single-surface BIA data and 32% more than models containing only length and weight data. While additional research is required to address the potential effects of methodological variation, bioelectrical impedance analysis shows promise as a way to provide high-quality, minimally invasive estimates of Dolly Varden lipid or moisture content in the field with only small increases in handling time.

Alaska

The effects of swimming exercise and dissolved oxygen on growth performance, fin condition and precocious maturation of early-rearing Atlantic salmon Salmo salar

Swimming exercise, typically measured in body-lengths per second (BL/s), and dissolved oxygen (DO), are important environmental variables in fish culture. While there is an obvious physiological association between these two parameters, their interaction has not been adequately studied in Atlantic salmon Salmo salar . Because exercise and DO are variables that can be easily manipulated in modern aquaculture systems, we sought to assess the impact of these parameters, alone and in combination, on the performance, health and welfare of juvenile Atlantic salmon. In our study, Atlantic salmon fry were stocked into 12 circular 0.5 m 3 tanks in a flow-through system and exposed to either high (1.5–2 BL/s) or low (<0.5 BL/s) swimming speeding and high (100% saturation) or low (70% saturation) DO while being raised from 10 g to approximately 350 g in weight. Throughout the study period, we assessed the impacts of exercise and DO concentration on growth, feed conversion, survival and fin condition. By study's end, both increased swimming speed and higher DO were independently associated with a statistically significant increase in growth performance ( p < .05); however, no significant differences were noted in survival and feed conversion. Caudal fin damage was associated with low DO, while right pectoral fin damage was associated with higher swimming speed. Finally, precocious male sexual maturation was associated with low swimming speed. These results suggest that providing exercise and dissolved oxygen at saturation during Atlantic salmon early rearing can result in improved growth performance and a lower incidence of precocious parr.

Aquaculture Research

Physiological response of largemouth bass to angling stress

The physiological effects of catch-and-release fishing on largemouth bass Micropterus salmoides from Lake Powell and Mantua Reservoir, Utah, were evaluated, and an estimate of the time needed for recovery from hooking stress was obtained. Fatigue in Lake Powell fish, as indicated by elevated blood lactate, was directly proportional to hooking time (1–5 min) and water temperature, but recovery from the hyperlacticemia was relatively rapid (about 24 h). Hyperglycemia, an indicator of stress hormone production, did not occur in largemouth bass hooked and played for 1–5 min in the coldest water (11–13°C), was moderate in fish hooked and played at l6–20°C, and was severe in fish played for 5 min at 28–30°C. Fish held for recovery in live cages suffered further hyperglycemia, presumably because of the stress of confinement. Ionoregulation, as indicated by relatively stable plasma chloride values, was not immediately affected in largemouth bass caught at water temperatures of 11–13°C or 28–30°C, but an unusual hyperchloremia developed in fish hooked and played at 16–20°C. During recovery, the expected progressive hypochloremia developed. Plasma osmolality was somewhat affected by hooking at all water temperatures tested, but recovery was almost complete within about 8 h. Mantua Reservoir fish were hooked and played only at water temperatures of 23–26°C. The hyperlacticemia and hyperglycemia that occurred were generally more severe than in the Lake Powell fish hooked and played at either 16–20°C or 28–30°C. However, effects on plasma chloride and osmolality were similar to those occurring in Lake Powell fish.

Transactions of the American Fisheries Society

Seventy-five years of science—The U.S. Geological Survey’s Western Fisheries Research Center

As of January 2010, 75 years have elapsed since Dr. Frederic Fish initiated the pioneering research program that would evolve into today’s Western Fisheries Research Center (WFRC). Fish began his research working alone in the basement of the recently opened Fisheries Biological Laboratory on Lake Union in Seattle, Washington. WFRC’s research began under the aegis of the U.S. Fish and Wildlife Service and ends its first 75 years as part of the U.S. Geological Survey with a staff of more than 150 biologists and support personnel and a heritage of fundamental research that has made important contributions to our understanding of the biology and ecology of the economically important fish and fish populations of the Nation. Although the current staff may rarely stop to think about it, WFRC’s antecedents extend many years into the past and are intimately involved with the history of fisheries conservation in the Western United States. Thus, WFRC Director Lyman Thorsteinson asked me to write the story of this laboratory “while there are still a few of you around who were here for some of the earlier years” to document the rich history and culture of WFRC by recognizing its many famous scientists and their achievements. This historyalso would help document WFRC’s research ‘footprint’ in the Western United States and its strategic directions. Center Director Thorsteinson concluded that WFRC’s heritage told by an emeritus scientist also would add a texture of legitimacy based on personal knowledge that will all-to-soon be lost to the WFRC and to the USGS. The WFRC story is important for the future as well as for historical reasons. It describes how we got to the place we are today by documenting the origin, original mission, and our evolving role in response to the constantly changing technical information requirements of new environmental legislation and organizational decision-making. The WFRC research program owes its existence to the policy requirements of Federal conservation legislation originating with the construction of Grand Coulee Dam in 1933. The research program was shaped by laws enacted in subsequent years such as the Federal Water Pollution Control Act (1972), National Environmental Policy Act (1973), Endangered Species Act (1974), and Northwest Power Planning Act (1980), to name only a few. The WFRC has not been constrained by direct management or regulatory responsibility for a particular fishery (such as providing sustainable catch limits data to a resource management structure). Thus, WFRC has been able to concentrate on scientific pursuits and information needs required by contemporary environmental legislation. Over the years, we have pioneered in several important areas of fisheries research including the diagnoses and control of diseases in economically important fish, effects of environmental alterations on the physiological quality and survival of Pacific salmon released from federal mitigation hatcheries, applications in biotelemetry, and the bioenergetics of predator-prey interactions in the Columbia River. The WFRC of today is a widely distributed organization in the Western United States. Knowledge of the historical connections and accomplishments of our predecessors is important beyond the sense of pride and unity it instills in the WFRC family of today. For example, a discerning reader will note the evolution of WFRC’s research from a single disciplinary focus (early era—hatchery disease problems), to multiple disciplines (middle to late era—species, populations, habitats; threatened and endangered species), to the present era (multidisciplinary and with increasing process focus). For the benefit of the current WFRC staff, more emphasis has been placed on the early years rather than on the present day because people are quite naturally more familiar with the recent past than with the research done during the first decades of WFRC’s existence. By every rational measure, the WFRC has evolved into a fisheries research organization well positioned to provide the biological information needed to support the continued conservation and management of our Nation’s living aquatic natural resources. The high standard of excellence that connects WFRC’s past to our present research program provides a firm foundation on which to base the work yet to be done. In another 75 years, WFRC will undoubtedly be a very different place than it is today, but its evolution will be forever rooted in the story of the research and of the people related here. More about the diverse fisheries research projects WFRC scientists are conducting today is available at WFRC’s website: http://wfrc.usgs.gov/.

Washington

Survival and spawning success of American shad (Alosa sapidissima) in varying temperatures and levels of glochidia infection

Temperature fluctuations and climate change impacts may substantially affect spawning success of fish, especially migratory species with a limited spawning window. Factors affecting American shad ( Alosa sapidissima ) spawning success and survival were investigated at different temperatures and periods (peak- and late-spawning periods) during the Connecticut River, USA, spawning migration in 2017. Wild caught American shad were exposed to constant temperatures regimes of 15, 18, 21, 24 and 27 °C for 2 weeks. During the peak-spawning period, an increase in temperature (15–24 °C) was shown to increase spawning success factors, including spawning probability, number of eggs, and fertilization success, but decreased egg size. Temperatures between 18 and 27 °C did not affect these factors during the late-spawning period. Glochidia infection by the alewife floater ( Anodonta implicata ) was much higher in the late-spawning period and significantly decreased the survival of American shad. Further research should investigate the parasite-host relationship between the alewife floater and American shad to determine annual variability of glochidia infections and how they affect American shad from physiological and passage perspectives. Higher temperatures were shown to increase spawning success of American shad during the peak-spawning period, but temperature had no effect during the late-spawning period. However, any effect during the late-spawning period may have been masked by a high level of glochidia infection.

Fish Physiology and Biochemistry

Concentration of organic contaminants in fish and their biological effects in a wastewater-dominated urban stream

Data are presented on the concentrations of alkylphenol and alkylphenol ethoxylates (APEs) and persistent organic compounds in largemouth bass collected from a waste-water dominated stream in downtown Chicago. The fish residue concentrations of APEs are compared to concentrations of the APEs in the water that were collected at weekly intervals over two months bracketing the fall (2006) and a spring (2007) fish collection. The concentrations of APEs were significantly higher in the spring-collected fish (5.42 μg/g) versus the fall (0.99 μg/g) tand these differences were shared by differences in the water concentrations (spring — 11.47 versus fall — 3.44 μg/L). The differences in water concentration were negatively correlated with water temperatures observed over the two sampling times. Fish residue concentrations of persistent organic compounds (PCBs, PBDEs, toxaphene, and many legacy pesticides including the DDT family) did not vary from fall to spring. Some of these residue concentrations were comparable to the highest NPE (nonylphenol ethoxylate) homologue concentrations, e.g. NP1EO was 3.5 μg/g in the bass for the spring, the PBDE-congener 47 and p,p′-DDE averaged 1.0 μg/g and 0.5 μg/g, respectively, over both seasons. All the other persistent single-analyte concentrations were lower. Biological endpoints for endocrine effects measured in the same fish showed that there was an apparent positive correlation for physiological effects based on increased vitellogenin levels in males versus concentration of NPEs; however there were no observable histological differences in fall versus spring fish samples.

Science of the Total Environment