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Guidance for assessing interregional ecoystem service flows

Ecosystem services (ES) assessments commonly focus on a specific biophysical region or nation and take its geographic borders as the system boundary. Most geographical regions are, however, not closed systems but are open and telecoupled with other regions through ES imports and exports, which are mediated by fluxes of matter, energy and information. Interregional ES flows are often linked to national economies and may affect issues of national security and global equity. To date, however, methodologies for interregional ES flow assessments have been published in dispersed literature. This paper provides a three-step guidance for the assessment of four different types of interregional ES flows (traded goods, passive biophysical flows, species migration and dispersal and information flows), in order to complement national ecosystem assessments. The three steps are to (i) define the goal and scope of interregional ES flow assessments, (ii) quantify the interregional ES flows using a tiered approach and (iii) interpret results in terms of uncertainties, consequences and governance options. We compile different indicators for assessing interregional ES flows and evaluate their suitability for national ES assessments. Finally, in order to value the various outcomes of telecouplings, including their implications for human well-being, we relate the interregional ES flow indicators to the Sustainable Development Goals. We discuss how regional studies can assess a country‘s dependence and impact on "overseas" ES. This guidance towards systematic assessment of interregional ES flows provides a first step to measure and quantify externalised environmental costs and can contribute to the development of indicators to address interregional imbalances in trade, foreign policy and beyond.

Ecological Indicators

Integrating monitoring and modeling information to develop an indicator of watershed progress toward nutrient reduction goals

Eutrophication has been a major environmental issue in many coastal and inland ecosystems, which is primarily attributed to excessive anthropogenic inputs of nutrients. Restoration efforts have therefore focused on the reduction of watershed nutrient loads, including in the Chesapeake Bay (USA). To facilitate watershed management, watershed models are often developed and used to assess the expected impact of scenarios of past and future management policies and practices and the impact of watershed conditions. However, the level of load reductions estimated using monitoring data often does not match with model predictions, which may cast doubt on the effectiveness of the restoration efforts, the reliability of the model, and the prospect of achieving pre-established reduction goals. To better reconcile such inconsistencies between expectation (i.e., modeling estimates) and reality (i.e., monitoring information), a watershed-wide indicator was developed for the Chesapeake Bay watershed to explicitly quantify the progress toward nutrient reduction goals in the context of the Chesapeake Bay Total Maximum Daily Load (TMDL). Results of the indicator show that since 1995 long-term progress has been made toward the TMDL planning targets for both nitrogen and phosphorus. Specifically, management practices that are implemented and realized (in monitoring data) have been increasing over time, whereas management practices that need to be implemented in the future to meet the goals have been decreasing. In addition, the progress of nutrient reduction toward meeting the goals has varied with source sectors and watershed locations: i.e., point source management has been fully or nearly fully implemented, whereas nonpoint source management has been implemented by 50%-70%. In summary, this indicator, which is largely based on monitoring data, can provide at least four benefits: (1) evaluating the validity of the modeled estimates of nutrient reductions by comparing them to monitoring information; (2) placing the monitored riverine trends into a management context; (3) comparing progress between different nutrient source sectors and watershed locations; and (4) facilitating communication of the progress to the Chesapeake Bay Program Partnership and the public. Although we focus on the indicator development and interpretation for the Chesapeake Bay watershed, the framework can be transferred to watersheds within and beyond this watershed, where similar modeling and monitoring information exists, to gauge expectations on the trajectory and pace of the progress toward meeting restoration goals.

Chesapeake Bay watershed

Protocol for Monitoring Fish Assemblages in Pacific Northwest National Parks

Rivers and streams that drain from Olympic, Mount Rainier, and North Cascades National Parks are among the most protected corridors in the lower 48 States, and represent some of the largest tracts of contiguous, undisturbed habitat throughout the range of several key fish species of the Pacific Northwest. These watersheds are of high regional importance as freshwater habitat sanctuaries for native fish, where habitat conditions are characterized as having little to no disturbance from development, channelization, impervious surfaces, roads, diversions, or hydroelectric projects. Fishery resources are of high ecological and cultural importance in Pacific Northwest National Parks, and significantly contribute to economically important recreational, commercial, and tribal fisheries. This protocol describes procedures to monitor trends in fish assemblages, fish abundance, and water temperature in eight rivers and five wadeable streams in Olympic National Park during summer months, and is based on 4 years of field testing. Fish assemblages link freshwater, marine, and terrestrial ecosystems. They also serve as focal resources of national parks and are excellent indicators of ecological conditions of rivers and streams. Despite the vital importance of native anadromous and resident fish populations, there is no existing monitoring program for fish assemblages in the North Coast and Cascades Network. Specific monitoring objectives of this protocol are to determine seasonal and annual trends in: (1) fish species composition, (2) timing of migration of adult fish, (3) relative abundance, (4) age and size structure, (5) extent of non-native and hatchery fish, and (6) water temperature. To detect seasonal and annual trends in fish assemblages in reference sites, we rely on repeated and consistent annual sampling at each monitoring site. The general rationale for the repeated sampling of reference sites is to ensure that we account for the high interannual variability in fish movements and abundances in rivers. One underlying assumption is that the monitoring program is designed in perpetuity, and consequently our capability to detect trends substantially increases with time. The protocol describes sampling designs, methods, training procedures, safety considerations, data management, data analysis, and reporting. The allocation of sampling effort represents a balance between ecological considerations, a sound monitoring approach, and practical limitations caused by logistical constraints and a limited annual budget of $55,000. The widespread declines of native fish species in western North America highlights the importance and urgency of understanding trends in fish assemblages from undisturbed habitats. Seasonal and annual trends in fish assemblages will provide insights at the individual, population, and assemblage level. This protocol will allow managers to detect increases and decreases in abundance of priority management species, and occurrence of non-native, hatchery, and federally listed fish. The detection of trends in fish assemblages will allow for specific management actions that may include: implementation of more appropriate fishing regulations, evaluation of existing hatchery releases, control of non-native fish species, and prioritization of habitat restoration projects. Dissemination and communication of scientific findings on North Coast and Cascades Network fish assemblages will be a core product of this protocol, which will have much relevance to decision makers, park visitors, researchers, and educators.

Techniques and Methods

Evidence for interactions among environmental stressors in the Laurentian Great Lakes

Co-occurrence of environmental stressors is ubiquitous in ecosystems, but cumulative effects are difficult to predict for effective indicator development. Individual stressors can amplify (synergies) or lessen (antagonisms) each other's impacts or have fully independent effects (additive). Here we use the Laurentian Great Lakes, where a multitude of stressors have been studied for decades, as a case study for considering insights from both a systematic literature review and an expert elicitation (or structured expert judgment) to identify stressor interactions. In our literature search for pairs of stressors and interaction-related keywords, relatively few studies (9%, or 6/65) supported additive interactions with independent stressor effects. Instead, both antagonisms (42%, or 27/65) and synergies (49%, or 32/65) were common. We found substantial evidence for interactions of invasive dreissenid mussels with nutrient loading and between pairs of invasive species (predominantly dreissenids × round goby), yet both sets of records included mixtures of synergies and antagonisms. Complete quantification of individual and joint effects of stressors was rare, but effect sizes for dreissenid mussels × nutrient loading supported an antagonism. Our expert elicitation included discussion in focus groups and a follow-up survey. This process highlighted the potential for synergies of nutrient loading with dreissenid mussels and climate change as seen from the literature review. The elicitation also identified additional potential interactions less explored in the literature, particularly synergies of nutrient loading with hypoxia and wetland loss. To stimulate future research, we built a conceptual model describing interactions among dreissenid mussels, climate change, and nutrient loading. Our case study illustrates the value of considering results from both elicitations and systematic reviews to overcome data limitations. The simultaneous occurrence of synergies and antagonisms in a single ecosystem underscores the challenge of predicting the cumulative effects of stressors to guide indicator development and other management and restoration decisions.

Ecological Indicators

Efficacy of eDNA as an early detection indicator for Burmese pythons in the ARM Loxahatchee National Wildlife Refuge in the Greater Everglades Ecosystem

Environmental DNA (eDNA) detection of invasive species can be used to delimited occupied ranges and estimate probabilities to inform management decisions. Environmental DNA is shed into the environment through skin cells and bodily fluids and can be detected in water samples collected from lakes, rivers, and swamps. In south Florida, invasive Burmese pythons occupy much of the Greater Everglades in mostly inaccessible habitat and are credited with causing severe declines of native species’ populations. Detection of Burmese pythons by traditional methods, such as trapping and visual searching, have been largely ineffective, making eDNA a superior method for differentiating invaded habitat. We adapted a quantitative PCR eDNA assay for droplet digital PCR, a state-of-the-art method that improves precision and accuracy. From August 2014 to October 2016, locations in and around Arthur R. Marshall Loxahatchee National Wildlife Refuge in southeast Florida were surveyed for Burmese python eDNA. The Refuge is maintained to provide water storage and is considered one of the last remnants of the northern Everglades wetlands. Positive eDNA detections were made at each of the five sampling events, assessing a total of 399 samples, with moderate occurrence (ψ=58-91%) and detection (p=40-70%) probabilities, potentially reduced by high PCR inhibition-levels. The high occurrence rates and geographic distribution of the positive samples within the Refuge suggests a steady release of python eDNA from a resident Burmese python population and reduces support for primarily transport of eDNA through boats or flowing water from the north. The first confirmed sighting of a Burmese python in the Refuge occurred in September 2016, after eDNA testing had indicated the presence of pythons. An established population is not expected this far north, however, the detections likely indicate northern range limit of a consistent population at Loxahatchee on the eastern side of the Florida peninsula. Our study demonstrates the benefit of eDNA for determining more accurate range limits and expansion information for Burmese pythons, as well as laying the foundation for the assessment of control efforts.

Florida

Lessons learned from 20 y of monitoring suburban development with distributed stormwater management in Clarksburg, Maryland, USA

Urban development is a well-known stressor for stream ecosystems, presenting a challenge to managers tasked with mitigating its effects. For the past 20 y, streamflow, water quality, geomorphology, and benthic communities were monitored in 5 watersheds in Montgomery County, Maryland, USA. This study presents a synthesis of multiple studies of monitoring efforts in the study area and new analysis of more recent monitoring data to document the primary lessons learned from monitoring. The monitored watersheds include a forested control, an urban control with centralized stormwater management, and 3 suburban treatment watersheds featuring low-impact development and a high density of infiltration-focused stormwater facilities distributed across the watershed. Treatment watersheds were monitored before development, during construction, and after development. Monitoring was initiated to inform adaptive management of stormwater and impervious cover limits within the study area, with a focus on the impacts of distributed stormwater management. Results from our synthesis indicate that distributed stormwater management is advantageous compared with centralized stormwater management in numerous ways. Hydrologic benefits were greater with distributed stormwater infrastructure, demonstrating the ability to mitigate runoff volumes and peak flows and, for small storms, replicate predevelopment conditions. Baseflow temporarily increased during the construction phase in the treatment watersheds. Water-quality benefits were mixed, with declines in baseflow nitrate concentrations but limited changes to nitrate export and increases in specific conductance after development. Substantial topographic changes occurred during construction in the treatment watersheds, including changes within the riparian zone, despite riparian buffer protections. Ecological monitoring indicated that even though index of biotic integrity scores rebounded in some cases, sensitive benthic macroinvertebrate families did not fully recover in the treatment watersheds. Lessons learned from this synthesis highlight the importance of tracking multiple indicators of stream health and considering past land use and that more stormwater facilities distributed across the watershed is beneficial but cannot mitigate the effects of all urban stressors on aquatic ecosystems.

Maryland

A strategy for defining the reference for land health and degradation assessments

Much of the confusion about the definition of reference conditions for land health and degradation assessments is due to differences in policy and management objectives. Selection of a historic reference where it is not necessary, such as in the definition of future land degradation neutrality, can add significant cost and uncertainty to land management projects that require some knowledge of the current status of the land relative to its potential. This paper (1) provides a review of conditions under which historic reference information is and is not required to meet management and policy objectives, (2) summarizes current approaches to defining the reference for land health and degradation assessments, and (3) presents a protocol, “Describing Indicators of Rangeland Health” (DIRH) for collecting and organizing data that can be used to define a historic reference. This protocol builds on the framework and indicators presented in the “Interpreting Indicators of Rangeland Health” (IIRH). IIRH uses a combination of scientific and local knowledge to generate soil- and climate-specific assessments of three attributes of land health. It is used in a number of countries. In the United States, data are aggregated over 30,000 locations to provide national assessments.

Ecological Indicators

Amphibian population responses to mitigation: Relative importance of wetland age and design

Wetland creation is a common practice to mitigate for the loss of natural wetlands. However, there is still uncertainty about how effectively created wetlands replace habitat provided by natural wetlands. This uncertainty is due in part because post-construction monitoring of biological communities, and vertebrates especially, is rare and typically short-term (<5 years). We estimated occupancy of 4 amphibian species in 8 created mitigation wetlands, 7 impacted wetlands, and 7 reference wetlands in the Greater Yellowstone Ecosystem in Wyoming, USA. Mitigation wetlands were created to replace wetland habitat that was lost during road construction and ranged in age from 1 to 10 years when sampled. Impacted wetlands were natural wetlands partially filled by road construction and were adjacent to a highway. We sampled for amphibian larvae during 6 summers from 2013 to 2020 and used multi-species occupancy models that estimated detection and occupancy of each of 4 amphibian species to determine how amphibian responses changed over time, especially in mitigation wetlands. Occupancy did not differ between impacted and reference wetlands for any of the 4 amphibian species. Western Toads ( Anaxyrus boreas ) were most common (although briefly) in created wetlands, and occupancy of Columbia Spotted Frogs ( Rana luteiventris ), Western Tiger Salamanders ( Ambystoma mavortium ), and Boreal Chorus Frogs ( Pseudacris maculata ) was lower in created wetlands than in impacted or reference wetlands. Individual wetland area was positively associated with occupancy for all 4 species and wetland vegetation cover was positively associated with Boreal Chorus Frog and Columbia Spotted Frog occupancy; these results emphasize the importance of design characteristics when planning mitigation wetlands. The link between wetland age and occupancy was complex and included threshold and quadratic relationships for three of the four species, but only Boreal Chorus Frog occupancy was still increasing slowly at the end of our study. Our results indicate created wetlands did not attain the suitability of impacted and natural wetlands for local amphibians, even several years after construction. The complex relationships between wetland age and species-specific occupancy illustrate the importance of long-term monitoring in describing population responses to the construction of wetlands as mitigation for wetland loss.

Ecological Indicators

A water quality barometer for Chesapeake Bay: Assessing spatial and temporal patterns using long-term monitoring data

This paper develops a barometer that indexes water quality in the Chesapeake Bay and summarizes quality over spatial regions and temporal periods. The barometer has a basis in risk assessment and hydrology, and is a function of three different metrics of water quality relative to numerical criteria: relative frequency of criterion attainment; magnitude of deviation from a numerical criterion; and duration of criterion attainment. Metrics associated with these features are calculated at the station level, allowing flexibility for simultaneously evaluating multiple stressors, different designated uses, and physical characteristics of the water. The barometer score is then created as a geometric mean of the three metrics. The water quality barometer (WQB) station scores may be spatially aggregated to report habitat scores across a spectrum of spatial resolutions (e.g., management segment, tidal subsystem, or the whole tidal bay). Dissolved oxygen measurements in the Chesapeake Bay collected during summer seasons of 1985 to 2020 are used to evaluate water quality. The WQB score and its bootstrapped confidence interval are reported at the station, segment, tidal subsystem and whole tidal bay levels. Notably, water quality interpreted through application of the WQB with dissolved oxygen concentration data and averaged over the 29-year period of record is good (i.e. protects aquatic living resources) in tributaries such as the James River, Rappahannock River and others; but is not as good in areas such as the Upper Tributaries and the York River. Recent summaries indicate that while the water quality is improving in much of the bay and its tidal tributaries, however, there is an indication of decline in quality in the period 20182020, especially in the upper regions of the Bay. The barometer is designed around using the time series data produced by the Chesapeake Bay Programs annual monitoring strategy; the approach has application to other large water bodies with large scale monitoring programs with extended time series or for integrating information from environmental sensor systems.

Maryland, Virginia

Development of vegetation based soil quality indices for mineralized terrane in arid and semi-arid regions

Soil quality indices (SQIs) are often management driven and attempt to describe key relationships between above- and below-ground parameters. In terrestrial systems, indices that were initially developed and modified for agroecosystems have been applied to non-agricultural systems in increasing number. We develop an SQI in arid and semi-arid ecosystems of the Western US impacted by different types of geologic mineralization using the relationship between vegetation community parameters and soil abiotic and biotic properties. We analyze these relations in soils associated with three different mineralization types: podiform chromite, Cu/Mo porphyry, and acid-sulfate gold vein systems at four different sites in California and Nevada. Soil samples were collected from undisturbed soils in both mineralized and nearby unmineralized substrates as well as from waste rock and tailings. Aboveground net primary productivity (ANPP), canopy cover and shrub density were measured for the vegetative communities. Minimum data sets were developed based on correlations between the soil and vegetation parameters, refined using principal components analysis, scored using non-linear functions, and combined into an overall SQI. The indices are comprised of one or two microbial parameters and three to six abiotic parameters, the latter consisting of nutrients and metals. Given the preliminary development of this approach, the parameters and combinations to arrive at an SQI for a given site cannot at this time be correlated or compared with that of another site. This SQI approach provides a means of quantifying disturbed ecosystem recovery resulting from mining, and could be applied to other disturbances in a way that readily distills the information for potential use by land managers. However, severely disturbed areas with little to no aboveground biomass, such as unreclaimed tailings, have likely crossed an ecological threshold that precludes the use of this type of monitoring tool.

California

A targeted annual warning system developed for the conservation of a sagebrush indicator species

A fundamental goal of population ecologists is to identify drivers responsible for temporal variation in abundance. Understanding whether variation is associated with environmental stochasticity or anthropogenic disturbances, which are more amenable to management action, is crucial yet difficult to achieve. Here, we present a hierarchical monitoring framework that models rates of change in abundance from spatially structured populations and identifies when local declines fall out of synchrony with trends at larger spatial scales. Importantly, the framework provides signals that alert managers to the categorical significance of observed declines while avoiding signals where declines result from drivers operating at larger spatial scales (e.g., periodic reductions in primary productivity owing to drought). We demonstrate utility through application to a rapidly declining sagebrush ( Artemisia spp.) indicator species (greater sage-grouse; Centrocercus urophasianus ) using 30 years (1990–2019) of count data collected from greater than 4,400 leks (habitual breeding sites) distributed across the western United States. Results revealed population declines, immediately preceding triggers (2–4-year period), ranging between 58 and 68%. Conversely, population trends unassociated with triggers showed little-to-no sign of decline. Retrospective application of the monitoring framework indicated an average annual rate of 1.7% of leks or 1.3% of neighborhood clusters (lek aggregations) would have required management intervention to reverse range-wide declines and stabilize the U.S. population as a whole.

Ecological Indicators

Estimating indicators of cyanobacterial harmful algal blooms in New York State

Cyanobacteria harmful algal blooms (cyanoHABs) are a global concern for aquatic ecosystem and human health. Limited funding for monitoring programs and inconsistent determination of cyanoHAB occurrence present challenges for identifying commonly effective variables for characterizing cyanoHABs and the development of generalized models. We compiled a combination of water quality measurements, lake morphology, climatology, remote sensing data, and observations of cyanoHAB occurrence across New York State and used this dataset to develop two sets of predictive models. The first model predicted chlorophyll a , a common indicator of algal biomass, and assessed the importance of variables for modeled predictions. The most important variables were then used in a second set of models to classify cyanoHAB occurrence. The irradiance attenuation coefficient ( K d ), which was estimated from Secchi depth measurements, and total phosphorus were the two most important variables for predicting chlorophyll a . The second model examined several variables for their ability to classify cyanoHAB occurrence. Predicted cyanoHAB occurrence based on thresholds of chlorophyll a , K d , or total nitrogen all had moderate agreement and were able to correctly classify approximately 70% of observed cyanoHABs. Our analysis indicated that multiple data types were important for predicting chlorophyll a statewide and that simple widely available water quality parameters could classify cyanoHABs occurrence with reasonable accuracy. Identifying variables that can be monitored with increased frequency and decreased latency to detect cyanoHAB occurrence will better inform water managers and provide valuable additional data for further refining predictive models of the likelihood of cyanoHABs occurrence.

New York

Small waterbodies of large conservation concern: Towards an integrated approach to more accurately measuring surface water dynamics

Millions of small waterbodies are dispersed throughout the middle of the North American continent, and billions of dollars have been invested to conserve, restore, and manage these waterbodies in the 20th and 21st centuries. Small waterbody conservation has been supported by different stakeholders aiming at improving water quality, enhancing floodwater storage, and supporting migratory bird breeding habitat. Conservation agencies are using hydrological and biological monitoring, modeling, and mapping to adaptively manage small waterbodies in the face of stressors such as invasive species and climate change. As remote sensing estimates of small waterbody surface water extent have become easier to access, understanding the capabilities and limitations of using remote sensing, especially in areas lacking surface water monitoring, is important for conservation decision making. Here, we used in situ monitoring and process-based hydrological modeling to explore remote sensing accuracy, especially related to waterbody size, emergent aquatic vegetation cover, and climatic conditions. Overall, we found that the accuracy of satellite and aerial imagery surface water mapping approaches vastly decreased for waterbodies smaller than 2 ha. We also found emergent vegetation could be masking surface water in waterbodies larger than 2 ha and that accuracy of some remote sensing estimates may decrease during wetter climatic periods. These results indicate that sensors commonly used for surface water applications alone may not be able to accurately detect small waterbody surface water, which supports the need for combining monitoring and modeling to understand how small waterbodies may respond to future changes in climate and land use.

Minnesota

Contribution of fish community metrics to the index of biotic integrity in two Ozark rivers

Flow regime, energy source, water quality, and habitat structure within stream systems play major roles in controlling the structure of resident fish communities (Gorman and Karr, 1978; Karr and Dudley, 1981; Schlosser, 1982). Based on the hypothesis that there are predictable relationships between stream variables and fish community structure, overall stream condition has been assessed using indices such as the Index of Biotic Integrity (IBI) (Karr, 1981; Karr et al., 1986). The IBI integrates a wide range of information pertaining to fish community structure that reflects the prevailing chemical, physical, and biological condition of the stream. Assessments made using the IBI have been correlated to actual stream conditions and appear to be sensitive to a wide range of environmental degradation (Fausch et al., 1990).

Ecological Indicators

Using satellite vegetation and compound topographic indices to map highly erodible cropland buffers for cellulosic biofuel crop developments in eastern Nebraska, USA

Cultivating annual row crops in high topographic relief waterway buffers has negative environmental effects and can be environmentally unsustainable. Growing perennial grasses such as switchgrass ( Panicum virgatum L.) for biomass (e.g., cellulosic biofuel feedstocks) instead of annual row crops in these high relief waterway buffers can improve local environmental conditions (e.g., reduce soil erosion and improve water quality through lower use of fertilizers and pesticides) and ecosystem services (e.g., minimize drought and flood impacts on production; improve wildlife habitat, plant vigor, and nitrogen retention due to post-senescence harvest for cellulosic biofuels; and serve as carbon sinks). The main objectives of this study are to: (1) identify cropland areas with high topographic relief (high runoff potentials) and high switchgrass productivity potential in eastern Nebraska that may be suitable for growing switchgrass, and (2) estimate the total switchgrass production gain from the potential biofuel areas. Results indicate that about 140,000 hectares of waterway buffers in eastern Nebraska are suitable for switchgrass development and the total annual estimated switchgrass biomass production for these suitable areas is approximately 1.2 million metric tons. The resulting map delineates high topographic relief croplands and provides useful information to land managers and biofuel plant investors to make optimal land use decisions regarding biofuel crop development and ecosystem service optimization in eastern Nebraska.

Nebraska

A tool to monitor hydrologic conditions on tree islands in the Everglades

Tree islands are patchy upland forested habitats in Florida's Everglades that face degradation and disappearance due to altered hydrologic patterns. The U.S. Geological Survey coordinated with the Miccosukee Tribe of Indians of Florida and the Seminole Tribe of Florida to co-develop a decision-support tool based on tree-island hydrologic conditions. Everglades managers can use this tool to help with restoration planning and water operations decisions that affect tree-island conditions. After a series of organized workshops and meetings, a list of hydrologic metrics was selected as indicators of tree-island health, including hydroperiod, number of days since last dry, and maximum water depth at the head of the island. As a result, a web application tool, called ETree, has been developed and is publicly available online. This web application provides data on daily metrics for the current Everglades water year and annual summaries for past years, beginning in 2000.

Florida

Use of tolerance values to diagnose water-quality stressors to aquatic biota in New England streams

Identification of stressors related to biological impairment is critical to biological assessments. We applied nationally derived tolerance indicator values for four water-quality variables to fish and benthic macroinvertebrate assemblages at 29 sites along an urban gradient in New England. Tolerance indicator values (TIVs), as biologically based predictors of water-quality variables, were determined for dissolved oxygen, nitrite plus nitrate (nitrate), total phosphorus, and water temperature for each site based on observed biological assemblages (TIVO), and for expected assemblages (TIVE). The quotient method, based on a ratio of the TIVs for observed and expected assemblages (tolerance units), was used to diagnose potential water-quality stressors. In addition, the ratio of measured water-quality values to water-quality criteria (water-quality units) was calculated for each water-quality variable to assess measured water-quality stressors. Results from a RIVPACS predictive model for benthic macroinvertebrates and Bray-Curtis dissimilarity for fish were used to classify sites into categories of good or impaired ecological condition. Significant differences were detected between good and impaired sites for all biological tolerance units (fish and benthic macroinvertebrate assemblages averaged) except for nitrate (P = 0.480), and for all water-quality units except for nitrate (P = 0.183). Diagnosis of water-quality stressors at selected sites was, in general, consistent with State-reported causes of impairment. Tolerance units for benthic macroinvertebrate and fish assemblages were significantly correlated for water temperature (P = 0.001, r = 0.63), dissolved oxygen (P = 0.001, r = 0.61), and total phosphorus (P = 0.001, r = 0.61), but not for nitrate (P = 0.059, r = -0.35). Differences between the two assemblages in site-specific diagnosis of water-quality stressors may be the result of differences in nitrate tolerance.

Ecological Indicators

Disturbance metrics predict a wetland Vegetation Index of Biotic Integrity

Indices of biological integrity of wetlands based on vascular plants (VIBIs) have been developed in many areas in the USA. Knowledge of the best predictors of VIBIs would enable management agencies to make better decisions regarding mitigation site selection and performance monitoring criteria. We use a novel statistical technique to develop predictive models for an established index of wetland vegetation integrity (Ohio VIBI), using as independent variables 20 indices and metrics of habitat quality, wetland disturbance, and buffer area land use from 149 wetlands in Ohio, USA. For emergent and forest wetlands, predictive models explained 61% and 54% of the variability, respectively, in Ohio VIBI scores. In both cases the most important predictor of Ohio VIBI score was a metric that assessed habitat alteration and development in the wetland. Of secondary importance as a predictor was a metric that assessed microtopography, interspersion, and quality of vegetation communities in the wetland. Metrics and indices assessing disturbance and land use of the buffer area were generally poor predictors of Ohio VIBI scores. Our results suggest that vegetation integrity of emergent and forest wetlands could be most directly enhanced by minimizing substrate and habitat disturbance within the wetland. Such efforts could include reducing or eliminating any practices that disturb the soil profile, such as nutrient enrichment from adjacent farm land, mowing, grazing, or cutting or removing woody plants.

Ecological Indicators