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Manipulation of the Symbiodiniaceae microbiome confers multigenerational impacts on symbioses and reproductive ecology of its Exaiptasia diaphana host

Symbiodiniaceae-associated microbiota strongly influence cnidarian symbioses. We systematically reduced the bacterial and fungal communities associated with Symbiodiniaceae to study potential effects on the cnidarian holobiont Exaiptasia diaphana (Aiptasia). Clonal anemones were inoculated with xenic Breviolum minutum (SSB01) and microbiome-manipulated cultures after antibacterial or antifungal treatment. The asexual reproduction of pedal laceration allowed for three generations of clonal aposymbiotic Aiptasia to be utilised in this study, from the initial adult generation (G0), to the first (G1), and second (G2) generation. We inoculated small and large G1 Aiptasia with SSB01 algae and monitored onset of symbiosis, rate of algal proliferation, and holobiont characteristics. Sequencing the 16S and 18S rRNA gene regions identified significant differences in the bacterial and fungal communities of the G0 and G1 generations, alongside differences between the size classes of small and large G1 anemones. The microbiome of larger G1 individuals was distinct to the smaller G1 anemones, suggesting a microbiome maturation process. Control Breviolum minutum cultures exhibited a significantly greater proliferation rate in large G1 anemones when compared to antibacterial or antifungal treated cultures, whereas the opposite trend was documented in the small G1 anemones. Although no differences were observed between algal photochemical parameters, or the growth and polyp activity of G1 juveniles, we observed a significant influence in the production of G2 clones between treatments. Overall, we provide strong ecological implications of manipulating Symbiodiniaceae microbiome, not for the algae themselves, but for the maturation of the host Aiptasia, as well as for the cnidarian holobiont over multiple generations.

The ISME Journal

Stratigraphic notes—Volume 1, 2022

This is the first volume in the U.S. Geological Survey (USGS) series of reports on stratigraphy entitled “Stratigraphic Notes,” which consists of short papers that highlight stratigraphic studies, changes in stratigraphic nomenclature, and explanations of stratigraphic names and concepts used on published geologic maps. “Stratigraphic Notes” is a long-term (multiyear), multivolume publication containing articles that address updates or revisions to stratigraphic nomenclature (and whose content ultimately will be incorporated by National Geologic Map Database personnel into Geolex, https://ngmdb.usgs.gov/Geolex/ ). We welcome papers for the “Stratigraphic Notes” series from geoscientists of the USGS, of State Geological Surveys, and from academicians. Papers can be submitted for publication in “Stratigraphic Notes” by contacting the USGS Geologic Names Committee ( gnc@usgs.gov ). As new “Stratigraphic Notes” volumes are published, links to the volumes will be posted at https://doi.org/10.3133/pp1879 . This first volume ("Stratigraphic notes—Volume 1, 2022") includes articles that provide guidance for those who wish to submit papers to “Stratigraphic Notes,” as well as information on how to make your manuscripts compliant for geologic names reviews and how to organize your paper’s content to facilitate inclusion of new or revised names in Geolex. This volume also includes some specific guidance on conducting geologic names reviews of geologic and hydrogeologic reports.

Professional Paper

Stratigraphic notes—Volume 2, 2025

This is the second volume in the U.S. Geological Survey (USGS) series of reports on stratigraphy entitled “Stratigraphic Notes,” which consists of short papers that highlight stratigraphic studies, changes in stratigraphic nomenclature, and explanations of stratigraphic names and concepts used on published geologic maps. “Stratigraphic Notes” is a long-term (multiyear), multivolume publication containing articles that address updates or revisions to stratigraphic nomenclature (and whose content ultimately will be incorporated by National Geologic Map Database personnel into Geolex, https://ngmdb.usgs.gov/Geolex/ ). We welcome papers for the “Stratigraphic Notes” series from geoscientists of the USGS, of State Geological Surveys, and from academicians. Papers can be submitted for publication in “Stratigraphic Notes” by contacting the USGS Geologic Names Committee ( gnc@usgs.gov ). As new “Stratigraphic Notes” volumes are published, links to the volumes will be posted at https://doi.org/10.3133/pp1879 .

Professional Paper

Stratigraphic notes—Volume 3, 2026

This is the third volume in the U.S. Geological Survey (USGS) series of reports on stratigraphy entitled “Stratigraphic Notes,” which consists of short papers that highlight stratigraphic studies, changes in stratigraphic nomenclature, and explanations of stratigraphic names and concepts used on published geologic maps. “Stratigraphic Notes” is a long-term (multiyear), multivolume publication containing articles that address updates or revisions to stratigraphic nomenclature (and whose content ultimately will be incorporated by National Geologic Map Database personnel into Geolex, https://ngmdb.usgs.gov/Geolex/ ). We welcome papers for the “Stratigraphic Notes” series from geoscientists of the USGS, of State Geological Surveys, and from academicians. Papers can be submitted for publication in “Stratigraphic Notes” by contacting the USGS Geologic Names Committee ( gnc@usgs.gov ). As new “Stratigraphic Notes” volumes are published, links to the volumes will be posted at https://doi.org/10.3133/pp1879 .

Professional Paper

Capitalization of positional (Lower/Middle/Upper) and temporal (Early/Middle/Late) adjectives in the names of formal chronostratigraphic and geochronologic units of the Phanerozoic

Many authors are understandably confused about the capitalization of the words “lower,” “middle,” “upper,” “early,” and “late.” Where these words are used simply as descriptive adjectives, they should be in lowercase; where they form the first word of a formal chronostratigraphic or geochronologic unit name, they should be in uppercase.

Professional Paper

The effects of the Chesapeake Bay impact crater on the geological framework and correlation of hydrogeologic units of the lower York-James Peninsula, Virginia

This report documents the highly variable structure, stratigraphy, and buried topography of the outer rim of the Chesapeake Bay impact crater created by its impact and burial. Lithologies of cores are correlated with borehole geophysical logs to characterize the physical properties of the stratigraphic units and their geophysical signatures. The correlation between cores, well cuttings, and borehole geophysical logs is augmented with seismic-reflection data, and these data are compiled into a lithostratigraphic cross section that illustrates the geological framework of the lower York-James Peninsula and immediate surrounding areas.

Virginia

Active volcanism on the Arabian Shield—Geology, volcanology, and geophysics of northern Harrat Rahat and vicinity, Kingdom of Saudi Arabia

Active volcanic systems pose serious hazards to people and property including inundation and incineration by lava, blanketing by tephra (volcanic ash), exposure to noxious volcanic gases, and damage from shallow earthquakes triggered by ascending molten material (magma). To improve understanding of volcanism and associated seismicity on the western Arabia Plate, the Saudi Geological Survey and the U.S. Geological Survey conducted a multi-year investigation of the northern Harrat Rahat volcanic field adjacent to the city of Al Madīnah al Munawwarah, Kingdom of Saudi Arabia. Project components included creation of a high-resolution digital topographic base; interpretation of eruptive history supported by detailed geologic mapping, paleomagnetism, and abundant high-precision geochronology of volcanic deposits; assessments of eruptive styles and volcanic hazards by physical volcanology; investigation of the origins of magmas in the mantle and of their differentiation in the crust revealed by chemical and isotopic petrology; gravity and magnetotelluric surveys to reveal crustal structures and to search for magma reservoirs; and regional and local seismic tomography and analyses of seismic hazards. Project results are presented in this Professional Paper as chapters written for technical scientific audiences.

Professional Paper

The Saudi Geological Survey-U.S. Geological Survey northern Harrat Rahat project—Styles, rates, causes, and hazards of volcanism near Al Madīnah al Munawwarah, Kingdom of Saudi Arabia

Active volcanic systems pose serious hazards to people and property including inundation and incineration by lava, blanketing by tephra (volcanic ash), exposure to noxious volcanic gases, and damage from shallow earthquakes triggered by ascending molten material (magma). To improve understanding of volcanism and associated seismicity on the western Arabia Plate, the Saudi Geological Survey and the U.S. Geological Survey conducted a multi-year investigation of the northern Harrat Rahat volcanic field adjacent to the city of Al Madīnah al Munawwarah, Kingdom of Saudi Arabia. Project components included creation of a high-resolution digital topographic base; interpretation of eruptive history supported by detailed geologic mapping, paleomagnetism, and abundant high-precision geochronology of volcanic deposits; assessments of eruptive styles and volcanic hazards by physical volcanology; investigation of the origins of magmas in the mantle and of their differentiation in the crust revealed by chemical and isotopic petrology; gravity and magnetotelluric surveys to reveal crustal structures and to search for magma reservoirs; and regional and local seismic tomography and analyses of seismic hazards. Project results are presented in this Professional Paper as chapters written for technical scientific audiences. This initial chapter introduces the project and briefly summarizes results in plain language for readers who have more general backgrounds.

Al Madīnah al Munawwarah

U.S. Geological Survey Integrated Water Availability Assessment—2010–20

This professional paper is a multichapter report that assesses water availability in the United States for water years 2010–20. This work was conducted as part of the fulfillment of the mandates of Subtitle F of the Omnibus Public Land Management Act of 2009 (Public Law 111-11), also known as the SECURE Water Act. As such, this work examines the spatial and temporal distribution of water quantity and quality in surface water and groundwater, as related to human and ecosystem needs and as affected by human and natural influences. Chapter A introduces the National Integrated Water Availability Assessment and provides important background and definitions for how the report characterizes water availability and its components. Chapter A also presents the key findings of Chapters B–F and thus acts as a summary of the entire report. Chapter B is a national assessment of water supply, which is the quantity of water supplied through climatic inputs. Chapter C is a national assessment of water quality, which is the chemical and physical characteristics of water. Chapter D assesses water use including withdrawals and consumptive use in the conterminous United States. Chapter E presents an analysis of factors affecting future water availability under changing climate conditions. The National Integrated Water Availability Assessment culminates with Chapter F, which is an integrated assessment of water availability that considers the amount and quality of water coupled with the suitability of that water for specific uses. Together, these six chapters constitute the National Integrated Water Availability Assessment for water years 2010–20.

Professional Paper

Paleomagnetism of the Harrat Rahat volcanic field, Kingdom of Saudi Arabia—Geologic unit correlations and geomagnetic cryptochron identifications

Paleomagnetic rock samples were collected from 173 drill sites in the Quaternary alkali basaltic volcanic field of northern Harrat Rahat, Kingdom of Saudi Arabia. Laboratory measurements on these samples established that lava flows and vent complexes—identified and mapped from field characteristics, rock types, and compositions as products of single or temporally close eruptions—typically record single, or very similar, directions of remanent magnetization. Correlations defined through geologic mapping, spatial association, geochemistry, geochronology, and identical mean remanent directions indicate at least 16 brief episodes of temporally clustered eruptions. These episodes had durations of a few centuries or less. Anomalous remanent magnetic directions were found for at least 13 mapped lavas of northern Harrat Rahat, which demonstrate that they were acquired during brief geomagnetic cryptochrons during the Brunhes Normal Polarity Chron. These uncharacteristic directions enhance the opportunity to identify common eruptive episodes, and to better understand and evaluate assessments of eruption ages based on 40 Ar/ 39 Ar geochronology. Combining paleomagnetic and regional archaeomagnetic results for the youngest eruptions allows us to evaluate their historical age assignments and, in one case, refute a previously assigned provisional age.

Harrat Rahat volcanic field

Prospectivity mapping for geologic hydrogen

Geologic, or naturally occurring, hydrogen has the potential to become a new, low-carbon, primary energy resource. Often referred to as “white” or “gold” hydrogen, this gas occurs naturally in the Earth’s subsurface, similar to petroleum resources. However, unlike petroleum, which releases carbon dioxide when burned, burning hydrogen only produces water as a byproduct. Exploration for geologic hydrogen remains in an early stage and discoveries of high concentrations of subsurface hydrogen are still relatively rare. To facilitate research and exploration for this potential resource, this report presents the first publicly available prospectivity map of geologic hydrogen accumulations in the conterminous United States. Prospective regions are those regions in which all major components necessary for a hydrogen accumulation likely are present—a source of sufficient hydrogen generation, porous reservoirs for storage, and seals to prevent leakage. The midcontinent region of the United States and the central California coast are revealed as having high prospectivity. This analysis also identifies previously unrecognized prospective regions that may be favorable due to long distance lateral migration of subsurface hydrogen, such as the offshore eastern seaboard of the United States, and can provide a linkage between surface observations of hydrogen degassing and far-field source regions. The methodology developed to create this map is expandable and flexible and may be adapted to incorporate new concepts in the hydrogen system and for application to other regions of the world.

conterminous United States

Groundwater-Surface water interactions research: Past trends and future directions

Interactions between groundwater and surface water sustain groundwater-dependent ecosystems and regulate river temperature and biogeochemical cycles, amongst many other processes. These interactions occur in freshwater environments including rivers, springs, lakes, and wetlands, and in coastal environments via tidal pumping, submarine groundwater discharge, and seawater intrusion. Here, we explore groundwater-surface water interactions research using bibliometric analyses of titles, abstracts, and keywords from 20,275 journal papers published between 1970 and 2023 extracted from Scopus. Analyses show that research into groundwater-surface water interactions is highly multi-disciplinary, with growing contributions from the social and biological sciences. The number of groundwater-surface water interactions papers is rapidly increasing with over 1200 papers published per year since 2020. Drawing on our data-driven approach and expert knowledge, we synthesise current research trends and identify critical future research directions. Despite the thousands of papers on groundwater-surface water interactions, important processes are still difficult to quantify or predict at meaningful spatial scales to inform water-resources management. We see benefits in future groundwater-surface water interactions research focusing on: (1) using new technologies including internet-of-things-based sensors, uncrewed vehicles, and remote-sensing approaches for data collection to inform groundwater-surface water interactions at large scales, (2) seeking approaches to upscale site-specific findings to better inform management, and (3) continuing the movement towards multi-disciplinary investigations to better inform the understanding of groundwater-surface water interactions and processes that will enable better management outcomes.

Journal of Hydrology

Eruptive history within the vicinity of Al Madīnah in northern Harrat Rahat, Kingdom of Saudi Arabia

The northernmost part of the Harrat Rahat volcanic field contains early Pleistocene to Holocene mafic eruptive products within the vicinity of the city of Al Madīnah, Kingdom of Saudi Arabia. A detailed geologic investigation into the eruptive history of a 570 square kilometer (km 2 ) area covering Al Madīnah and the surrounding area has yielded 33 mapped Quaternary volcanic units consisting of lava flows, scoria cones, and shield volcanoes. These eruptive products consist of continental, intraplate alkalic and minor transitional basalts, hawaiites, and a single mugearite that were emplaced from at least 1,014±14 thousand years ago (ka) to a single Holocene eruption in 1256 C.E. Lava flows are generally 10 to 15 kilometers (km) long (but can reach 23 km long), 1 to 3 km wide, and at least 10 meters thick. Most of the mapped units erupted episodically between 400 and 340 ka and 180 and 100 ka. Despite small individual volumes (less than 1 cubic kilometers dense rock equivalent), each unit represents eruption of a distinct magma batch that was strongly influenced by clinopyroxene, olivine, and plagioclase fractionation. Some of these units are interpreted to have undergone magma mixing pre- and (or) syneruptively. Integrating eruption ages, geochemistry, and paleomagnetic data yields evidence that some eruptions were temporally and (or) spatially clustered. Aligned scoria cones and elongate vent edifices were constructed atop fissure vent systems that reflect the local stress field, which controls dike ascent through the middle and upper crust.

Professional Paper

High-resolution magnetic survey using an unoccupied aerial vehicle to constrain buried lava flow geometry, volume, and eruptive history of Little Cones, Crater Flat, Nevada

Magnetic surveys are an important tool used to augment geologic mapping in distributed volcanic fields. Using magnetic anomalies, it is possible to model the geometry of shallowly buried volcanic features, such as conduits, sills, and lava flows. This subsurface mapping is important for understanding eruption dynamics and emplacement of lava flows, and it sometimes reveals buried volcanoes no longer visible at the surface. These data are critical to better interpret the numbers, styles, and magnitudes of eruptions in distributed volcanic fields and their associated volcanic hazards. New advances in unoccupied aerial vehicles (UAVs) offer an attractive middle range of resolution and aerial coverage between ground-based magnetic surveys and aeromagnetic surveys. Here, we present the results of a UAV fluxgate magnetic survey of the Little Cones, Nevada, scoria cones, which have been the target of previous ground and aeromagnetic surveys. The magnetic anomalies at Little Cones are of interest because the surrounding alluvium conceals lava flows that erupted from Little Cones, making it very difficult to understand the volume and morphology of lava flows from geologic mapping alone. Nonlinear inversion of UAV-collected magnetic data were used to model the thickness and morphology of buried Little Cones’ lava flows with higher precision than achieved previously. The sequence of events and calculated flow characteristics are then interpreted. The total volume of Little Cones, including concealed lava flows, is approximately 0.016 cubic kilometer, and the initial sheet flow erupted in less than 24 hours. The findings presented herein demonstrate that UAV-based magnetic surveys are a reliable method of data collection and an efficient alternative to other survey methods, facilitating development of a three-dimensional perspective of distributed volcanic fields.

Nevada

Resurvey of the Marble Canyon and Bridge Canyon dam sites in Grand Canyon National Park—Changes in sediment storage and evidence supporting the occurrence of bedrock incision through the mid-20th century

The Bureau of Reclamation developed an extensive plan for a network of dams, water tunnels, and hydropower plants in and around Grand Canyon, Arizona, in the 1940s through 1960s. The two largest of these planned dams were the Marble Canyon and Bridge Canyon Dams on the Colorado River. Though these dams were ultimately never built, Reclamation conducted extensive topographic, bathymetric, and subsurface exploration work at the sites proposed for these dams in the 1940s and 1950s. Resurveys of these dam sites were conducted between 1998 and 2021 to determine the changes in sediment storage at these dam sites caused by the upstream construction and operation of Glen Canyon Dam and by the recession of Lake Mead, the reservoir impounded by Hoover Dam. The resurveys of the Marble Canyon dam sites indicate that the post-1950s changes in sediment storage at these dam sites are broadly consistent with flux-based estimates of voluminous sand erosion from Marble Canyon since the 1963 closure of Glen Canyon Dam. These resurveys also suggest that the pre-dam longitudinal variation in sediment thickness over bedrock played a key role in determining the locations of the sand erosion induced by Glen Canyon Dam; more sand eroded from locations where more sand was present in the 1950s. The resurvey of the Bridge Canyon dam sites indicates that the Colorado River’s incision of the Lake Mead delta is regulated both by bed-sediment grain size and downstream hydraulic controls. Finally, analyses of bed-sediment thickness and sedimentological data at the dam sites, and observations of bed scour and gravel transport, suggest that sufficient bedrock was exposed to allow bedrock incision during commonly recurring pre-dam snowmelt floods that entrained small boulders into transport.

Arizona

Status of water-quality conditions in the United States, 2010–20

Degradation of water quality can make water harmful or unusable for humans and ecosystems. Although many studies have assessed the effect of individual constituents or narrow suites of constituents on freshwater systems, no consistent, comprehensive assessment exists over the wide range of water-quality effects on water availability. Using published studies, data, and models completed at regional or national scales in the United States during 2010–20, this chapter moves towards a comprehensive assessment by summarizing how selected anthropogenic and geogenic water-quality constituents affect national-scale water availability for human and ecosystem needs. Several types of human health, agricultural, ecological, and beneficial-use standards or thresholds were used to provide context for categorizing surface-water and groundwater quality. Water availability for human and ecological use is limited by elevated concentrations of geogenic and anthropogenic constituents in surface and groundwater. Elevated concentrations of five geogenic constituents (arsenic, manganese, strontium, radium, and adjusted gross alpha) are common in groundwater and collectively affect the drinking water supply to over 30 million people. Surface water sourced drinking water supplies are impaired in about a third of assessed stream miles, most commonly because of non-mercury metals and salinity. Health-based violations at community water systems may disproportionately affect socially vulnerable communities. Ecological water uses are predominantly limited by nutrients, sediment, temperature, pathogens, salinity, and pesticides. Water availability for human and ecological use is adversely affected by human activities including human contaminant sources (for example, wastewater, agriculture), processes (for example, dredging, groundwater pumping), or permanent landscape modifications (for example, dams, urbanization). Primary contaminant sources vary spatially and include fertilizer and manure, atmospheric deposition, wastewater treatment plants, urban land, and a range of natural sources. Contaminants of emerging concern, contaminants without regulatory thresholds, and mixtures of geogenic and anthropogenic water contaminants also contribute to ecological degradation and human exposure.

conterminous United States

Cambrian and Ordovician stratigraphy, conodont biostratigraphy, and microfacies analysis to support 1:24,000-scale geologic mapping of the southern Lake Champlain valley, New York and Vermont

Introduction Geologic mapping in the southern Lake Champlain valley of New York and Vermont (fig. 1) has required evaluation of stratigraphic nomenclature used since the early 1900s. The paleogeography of the Cambrian and Ordovician adjacent to the Adirondack Highlands, the high-relief mountains of Proterozoic igneous and metamorphic rocks of northern New York, has impacted the lithostratigraphic succession, facies changes, and several hiatuses that occur within the package of clastic and carbonate rocks that demonstrates overall deepening consistent from the southern to northern Appalachians. The stratigraphic nomenclature used for the geologic mapping of the Cambrian and Ordovician strata of the study area is derived from evaluation of names put forth by previous workers from areas of northern New York and western Vermont. Descriptions of type localities or type sections and other criteria set forth by the North American Stratigraphic Code (North American Commission on Stratigraphic Nomenclature [NACSN], 2021) were consulted. The North American Stratigraphic Code states that lithostratigraphic units, such as the fundamental unit, the formation, be defined by lithic character and be mappable (NACSN, 2021, Article 24). However, some early workers (Walcott, 1912; Kay, 1937; Fisher and Hanson, 1951; Oxley and Kay, 1959) have defined stratigraphic units based on fossil content, which may or may not be facies dependent, and hence may not be mappable as lithostratigraphic units. Although preservation of older names takes priority in stratigraphic nomenclature (NACSN, 2021, Article 7), some do not have adequately defined stratotypes or are located a distance away with different lithologies than that in the study area. Biostratigraphy can be a helpful tool for understanding correlations and facies changes. Herein, conodont biostratigraphy is utilized to date and correlate separate lithologic units. Further, microfacies analyses of units through inspection of petrographic thin sections provide paleogeographic information that helps in understanding the genesis of geologic units.

New York, Vermont