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Fishway Entrance Palisade

This technical report summarizes the work that was conducted by the University of Massachusetts Amherst and the United States Geological Survey (USGS), along with other project partners, on the Fishway Entrance Palisade (EP), a projected funded through the Department of Energy’s (DOE) funding opportunity titled ‘Innovative Solutions for Fish Passage at Hydropower Dams’ (DE‐FOA‐0001662). The period of performance ranged from September 1, 2018 through September 30, 2021. The EP is a novel fish passage engineering technology designed to provide more favorable entry conditions for fish and to reduce costs relative to conventional fishway auxiliary water systems (AWS). The EP project has four primary components. First, the Northeast United States Auxiliary Water Systems Database was created (Northeast Fishway Auxiliary Water Systems Database Section). The database, developed with material provided by the U.S. Fish and Wildlife Service, contains information on fishway type (e.g., lift, Denil, pool and weir) and Auxiliary Water System (AWS) details (e.g., water conveyance method, diffuser type) for 60 hydroelectric sites in the region. Findings indicate that nearly 4 out of every 10 fishway in the region is a fish lift and approximately 1 out of every 4 is a Denil ladder. The remainder are a mix of vertical slot fishways, pool and weirs, and Ice Harbor fishways. Furthermore, over half of all AWS systems use floor diffusers to discharge the auxiliary (or attraction) water into the entrance of a fishway, whereas only 14% use wall diffusers. Second, limited experiments on a conventional AWS with live, actively migrating fish were conducted at the USGS Easter Ecological Science Center (EESC) S.O. Conte Research Laboratory (Conventional Auxiliary Water System Experiments Section). This study determined how water velocity through a wall diffuser, without turning vanes or timber baffles to distribute the flow, affects the behavior and passage of adult American shad, a conservative surrogate species for migratory fish on the East Coast. Two gross diffuser velocity treatments were examined, 0.5 ft/s and 1.0 ft/s. These wall diffuser velocities represented current (0.5 ft/s) and past (1.0 ft/s) design criteria guidelines set forth by the USFWS North Atlantic-Appalachian Region (Rojas 2020; USFWS 2019). Six trials with a total of 151 American Shad were conducted in June of 2019 for the two treatments. No differences in American shad passage efficiency were discovered between the two treatments, while approximately 3 in every 4 attempts were successful at passing the diffuser. While these results may appear to indicate that the generally accepted gross wall diffuser velocity criteria for American shad of 0.5 ft/s could be safely increased to 1.0 ft/s, further analysis is warranted. Furthermore, it is unknown how other migratory and resident fish species that traverse these structures would be impacted by such a change. Studying the wall diffuser hydraulics led to an important AWS observation. Without turning vanes or timber baffles in this study, doubling the diffuser area was insufficient at producing the type of flow field change one may expect by halving the gross diffuser velocity. Instead, the flow fields throughout each treatments study area were similar, which led to similar results in shad performance. This not only highlights the importance of installing flow guidance devices like turning vanes, but also to the importance of properly maintaining them, which can be costly. Third, more expansive experiments on the novel EP were conducted in the spring of 2019 and 2021 (Fishway Entrance Palisade Experiments). The goal of this study was to determine how adult American shad responded to a variety of conditions at a full-scale EP. A total of six treatments were examined by changing the average auxiliary channel velocity between 1.0 and 5.0 ft/s in intervals of 1.0 ft/s and by inserting/removing an entrance gate at the opening of the fishway. Thirty trials with a total of 1,273 shad were conducted over the two years. In all treatments, at least ~7 out of every 10 fish successfully passed the EP diffuser and swam into the entrance channel within the 3.5-hour long trial, highlighting the general effectiveness of the novel AWS technology. In both study years, lower velocities through the EP diffuser led to increased shad performance, though performance peaked for the 2 ft/s velocity treatment. This treatment condition represents an approximate six-fold increase in gross diffuser velocity relative to conventional auxiliary water systems, which in turn presents opportunities for cost savings (e.g., reduction in diffuser size). Shad performance, in general, was worse in 2019 than in 2021, potentially due to the different run timing when our trials were conducted (2019 trials occurred near the end of the migration season, unlike in 2021). Treatments in 2019 had approximately a 20% reduction in entrance efficiency by the trial end, including a 16.7% drop for the 3 ft/s velocity treatment in 2019 relative to 2021 (the only carryover treatment between years). Lastly, adding an entrance gate caused a significant delay to entry. The time to 25% entry raised ~20 minutes from the near instantaneous 25% entry that was reported for the other treatments conducted in the same year (2021). Though by the end of the 3.5-hour trial, the overall entrance efficiency nearly matched those of the other 2021 treatments. The fourth and final component of the EP project was an economic analysis that focused on the cost of attraction and environmental flows (Modeling Power Generation Losses Due to Environmental and Fish Passage Attraction Flows at a Run-Of-River Hydroelectric Operation in the Northeast). The study assessed the economic impact of meeting environmental flow requirements at a representative hydroelectric facility and fish lift in the Northeast. An initial finding of the study was that there is a paucity of published data on the costs of meeting attraction and environmental flows. This is due, in part, to the proprietary nature of this data. To explore the costs associated with these flows, three types of environmental flows were assessed: upstream fishway attraction flows, downstream fishway attraction flows, and habitat maintenance minimum flows. A physics-based model was developed and calibrated with three years of hourly generation and flow data as inputs. Gage flow inputs were adjusted and used to calculate power generated. To address hydrologic variability, the model was executed to simulate 30 years of historical flows. Results indicate that both interannual and seasonal climatic factors impact the costs of meeting environmental flow requirements. Generation potential is most strongly curtailed during dry years in terms of maximizing the capacity factor (the percent of time a plant generates at capacity). Dry years, and especially dry summers, have the most significant costs associated with mitigation flows. Of the three types of flows, habitat flows are most costly in terms of power production, followed by upstream attraction flows. Downstream attraction flows are least costly. This finding is the likely result of differences in both flow rates and duration of the seasonal requirement for each flow. Overall, environmental flows represented a 2-12% loss in annual generation, but losses during a dry summer can reach over 20%.

Final Technical Report

Meteorologic, oceanographic, and geomorphic controls on circulation and residence time in a coral reef-lined embayment: Faga’alu Bay, American Samoa

Water circulation over coral reefs can determine the degree to which reef organisms are exposed to the overlying waters, so understanding circulation is necessary to interpret spatial patterns in coral health. Because coral reefs often have high geomorphic complexity, circulation patterns and the duration of exposure, or “local residence time” of a water parcel, can vary substantially over small distances. Different meteorologic and oceanographic forcings can further alter residence time patterns over reefs. Here, spatially dense Lagrangian surface current drifters and Eulerian current meters were used to characterize circulation patterns and resulting residence times over different regions of the reefs in Faga’alu Bay, American Samoa, during three distinct forcing periods: calm, strong winds, and large waves. Residence times varied among different geomorphic zones of the reef and were reflected in the spatially varying health of the corals across the embayment. The relatively healthy, seaward fringing reef consistently had the shortest residence times, as it was continually flushed by wave breaking at the reef crest, whereas the degraded, sheltered, leeward fringing reef consistently had the longest residence times, suggesting this area is more exposed to land-based sources of pollution. Strong wind forcing resulted in the longest residence times by pinning the water in the bay, whereas large wave forcing flushed the bay and resulted in the shortest residence times. The effect of these different forcings on residence times was fairly consistent across all reef geomorphic zones, with the shift from wind to wave forcing shortening mean residence times by approximately 50%. Although ecologically significant to the coral organisms in the nearshore reef zones, these shortened residence times were still 2–3 times longer than those associated with the seaward fringing reef across all forcing conditions, demonstrating how the geomorphology of a reef environment sets a first-order control on reef health.

Coral Reefs

Chasing a changing climate: Reproductive and dispersal traits predict how sessile species respond to global warming

Aim Studies of species' range shifts have become increasingly relevant for understanding ecology and biogeography in the face of accelerated global change. The combination of limited mobility and imperilled status places some species at a potentially greater risk of range loss, extirpation or extinction due to climate change. To assess the ability of organisms with limited movement and dispersal capabilities to track shifts associated with climate change, we evaluated reproductive and dispersal traits of freshwater mussels (Unionida), sessile invertebrates that require species‐specific fish for larval dispersal. Location North American Atlantic Slope rivers. Methods To understand how unionid mussels may cope with and adapt to current and future warming trends, we identified mechanisms that facilitated their colonization of the northern Atlantic Slope river basins in North America after the Last Glacial Maximum. We compiled species occurrence and life history trait information for each of 55 species, and then selected life history traits for which ample data were available (larval brooding duration, host fish specificity, host infection strategy, and body size) and analysed whether the trait state for each was related to mussel distribution in Atlantic Slope rivers. Results Brooding duration ( p < .01) and host fish specificity ( p = .02) were significantly related to mussel species distribution. Long‐term brooders were more likely than short‐term brooders to colonize formerly glaciated rivers, as were host generalists compared to specialists. Body size and host infection strategy were not predictive of movement into formerly glaciated rivers ( p > .10). Main conclusions Our results are potentially applicable to many species for which life history traits have not been well‐documented, because reproductive and dispersal traits in unionid mussels typically follow phylogenetic relationships. These findings may help resource managers prioritize species according to climate change vulnerability and predict which species might become further imperilled with climate warming. Finally, we suggest that similar trait‐based decision support frameworks may be applicable for other movement limited taxa.

Diversity and Distributions

Population size of snowy plovers breeding in North America

Snowy Plovers ( Charadrius nivosus ) may be one of the rarest shorebirds in North America yet a comprehensive assessment of their abundance and distribution has not been completed. During 2007 and 2008, 557 discrete wetlands were surveyed and nine additional large wetland complexes sampled in M&eacute;xico and the USA. From these surveys, a population of 23,555 (95% CI = 17,299 &ndash; 29,859) breeding Snowy Plovers was estimated. Combining the estimate with information from areas not surveyed, the total North American population was assessed at 25,869 (95% CI = 18,917 &ndash; 32,173). Approximately 42% of all breeding Snowy Plovers in North America resided at two sites (Great Salt Lake, Utah, and Salt Plains National Wildlife Refuge, Oklahoma), and 33% of all these were on wetlands in the Great Basin (including Great Salt Lake). Also, coastal habitats in central and southern Texas supported large numbers of breeding plovers. New breeding sites were discovered in interior deserts and highlands and along the Pacific coast of M&eacute;xico; approximately 9% of the North American breeding population occurred in M&eacute;xico. Because of uncertainties about effects of climate change and current stresses to breeding habitats, the species should be a management and conservation priority. Periodic monitoring should be undertaken at important sites to ensure high quality habitat is available to support the Snowy Plover population.

Waterbirds

Introduction to the special issue on the 2012 Haida Gwaii and 2013 Craig earthquakes at the Pacific–North America plate boundary (British Columbia and Alaska)

The 27 October 2012 M w 7.8 Haida Gwaii thrust earthquake and the 5 January 2013 M w 7.5 Craig strike‐slip earthquake are the focus of this special issue. They occurred along the transform boundary between the Pacific and North American plates (Fig. 1). The most identifiable feature of the plate boundary, the strike‐slip Queen Charlotte fault, might be viewed as typical of continent–ocean transform faults because it separates the continental crust of the North American plate from oceanic crust of the Pacific plate for most of its length. However, the current relative plate motion of about 5 cm/yr is highly oblique to the Queen Charlotte fault, causing a transpressive plate boundary in the region.

Alaska, British Columbia

Walden Pond, Massachusetts: Environmental setting and current investigations

Introduction Walden Pond, in Concord, Massachusetts, is famous among lakes because of its unique social history. Walden was the setting for American naturalist Henry David Thoreau's well-known essay 'Walden; or, Life in the Woods,' first published in 1854. Thoreau lived and wrote at Walden Pond from July 1845 to September 1847. In 'Walden,' Thoreau combined highly admired writing on Transcendental philosophy with pioneering observations of aquatic ecology and physical aspects of limnology, the study of lakes. Because Thoreau also defended so effectively the value of living close to nature in the Walden woods, the pond is considered by many to be the birthplace of the American conservation movement. Visitors come from all over the world to the pond, which has been designated a National Historic Landmark, and its fame has resulted in a major fund drive to preserve the surrounding woods. Walden Pond has no surfacewater inflow or outflow, and much of its ground-water contributing area likely is preserved within the Walden Pond Reservation area (fig. 1). Only 15 miles from Boston, the pond is unusually clear and pristine for an urban-area lake. However, point sources of nutrients near the pond, and a large annual visitor attendance, concentrated during the summer when the swimming beach (fig. 2) is open, may contribute a nutrient load sufficient to change the pond environment. The occurrence of nuisance algal species, a recent beach closing, and an awareness of water-quality problems suffered by other ponds in the region raise concerns about the risk of ecological change at Walden Pond. Despite the role of Walden Pond as a cultural and environmental icon, little is known about the pond's ecological features, such as its internal nutrient cycling or the structure of its food web, nor have consistent measurements been made to determine whether these features are changing or are stable. Production rates of aquatic plants in lakes and ponds naturally undergo a slow increase as plant nutrients, organic matter, and soil are leached and eroded from the surrounding watershed. This process is known as eutrophication. The term 'cultural eutrophication' refers to an accelerated form of the natural process in which extra soil and nutrients are derived from people's use of fertilizer, rerouting of surface drainage, and disposal of domestic and industrial waste. Cultural eutrophication can lead to excessive growth of aquatic plants, pond filling by decayed plants and eroded soils, reduced water clarity, and depletion of dissolved oxygen in deep water with subsequent loss of cold-water fish habitat. In order to document the longterm ecological health of Walden Pond, the U.S. Geological Survey (USGS), working in cooperation with the Massachusetts Department of Environmental Management (MDEM), is investigating factors that could contribute to cultural eutrophication of Walden. Through measurements of mass balance of nutrients and oxygen in the pond's deep water, the investigation will establish a baseline data set on Walden's trophic state, which is a measure of the pond's ability to support plant growth. The baseline data will be used to detect trends and give early warning of trophic changes or trophic response to pond remediation projects. This Fact Sheet provides background information on the environmental setting, limnological features, and cultural eutrophication of Walden Pond, and describes the joint USGS/MDEM study.

Massachusetts

The effect of residential development on ground-water quality near Detroit, Michigan

Two water-quality studies were done on the outskirts of the Detroit metropolitan area to determine how recent residential development has affected ground-water quality. Pairs of monitor and domestic wells were sampled in areas where residential land use overlies glacial outwash deposits. Young, shallow waters had significantly higher median concentrations of nitrate, chloride, and dissolved solids than older, deeper waters. Analysis of chloride/bromide ratios indicates that elevated salinities are due to human activities rather than natural factors, such as upward migration of brine. Trace concentrations of volatile organic compounds were detected in samples from 97 percent of the monitor wells. Pesticides were detected infrequently even though they are routinely applied to lawns and roadways in the study area. The greatest influence on ground-water quality appears to be from septic-system effluent (domestic sewage, household solvents, water-softener backwash) and infiltration of stormwater runoff from paved surfaces (road salt, fuel residue). No health-related drinking-water standards were exceeded in samples from domestic wells. However, the effects of human activities are apparent in 76 percent of young waters, and at depths far below 25 feet, which is the current minimum well-depth requirement.Two water-quality studies were done on the outskirts of the Detroit metropolitan area to determine how recent residential development has affected ground-water quality. Pairs of monitor and domestic wells were sampled in areas where residential land use overlies glacial outwash deposits. Young, shallow waters had significantly higher median concentrations of nitrate, chloride, and dissolved solids than older, deeper waters. Analysis of chloride/bromide ratios indicates that elevated salinities are due to human activities rather than natural factors, such as upward migration of brine. Trace concentrations of volatile organic compounds were detected in samples from 97 percent of the monitor wells. Pesticides were detected infrequently even though they are routinely applied to lawns and roadways in the study area. The greatest influence on ground-water quality appears to be from septic-system effluent (domestic sewage, household solvents, water-softener backwash) and infiltration of stormwater runoff from paved surfaces (road salt, fuel residue). No health-related drinking-water standards were exceeded in samples from domestic wells. However, the effects of human activities are apparent in 76 percent of young waters, and at depths far below 25 feet, which is the current minimum well-depth requirement.

Michigan

Influence of salinity on relative density of American crocodiles (Crocodylus acutus) in Everglades National Park: Implications for restoration of Everglades ecosystems

The status of the American crocodile (Crocodylus acutus) has long been a matter of concern in Everglades National Park (ENP) due to its classification as a federal and state listed species, its recognition as a flagship species, and its function as an ecosystem indicator. Survival and recovery of American crocodiles has been linked with regional hydrological conditions, especially freshwater flow to estuaries, which affect water levels and salinities. We hypothesize that efforts to restore natural function to Everglades ecosystems by improving water delivery into estuaries within ENP will change salinities and water levels which in turn will affect relative density of crocodiles. Monitoring ecological responses of indicator species, such as crocodiles, with respect to hydrologic change is necessary to evaluate ecosystem responses to restoration projects. Our objectives were to monitor trends in crocodile relative density within ENP and to determine influences of salinity on relative density of crocodiles. We examined count data from 12 years of crocodile spotlight surveys in ENP (2004 to 2015) and used a hierarchical model of relative density that estimated relative density with probability of detection. The mean predicted value for relative density (λ) across all surveys was 2.9 individuals/km (95% CI: 2.0 – 4.2); relative density was estimated to decrease with increases in salinity. Routes in ENP’s Flamingo/Cape Sable area had greater crocodile relative density than routes in the West Lake/Cuthbert Lake area and Northeast Florida Bay areas. These results are consistent with the hypothesis that restored flow and lower salinities will result in an increase in crocodile population size and provide support for the ecosystem management recommendations for crocodiles, which currently are to restore more natural patterns of freshwater flow to Florida Bay. Thus, monitoring relative density of American crocodiles will continue to be an effective indicator of ecological response to ecosystem restoration.

Florida

Mycoplasmosis and upper respiratory tract disease of tortoises: a review and update

Tortoise mycoplasmosis is one of the most extensively characterized infectious diseases of chelonians. A 1989 outbreak of upper respiratory tract disease (URTD) in free-ranging Agassiz's desert tortoises ( Gopherus agassizii ) brought together an investigative team of researchers, diagnosticians, pathologists, immunologists and clinicians from multiple institutions and agencies. Electron microscopic studies of affected tortoises revealed a microorganism in close association with the nasal mucosa that subsequently was identified as a new species, Mycoplasma agassizii . Over the next 24 years, a second causative agent, Mycoplasma testudineum , was discovered, the geographic distribution and host range of tortoise mycoplasmosis were expanded, diagnostic tests were developed and refined for antibody and pathogen detection, transmission studies confirmed the pathogenicity of the original M. agassizii isolate, clinical (and subclinical) disease and laboratory abnormalities were characterized, many extrinsic and predisposing factors were found to play a role in morbidity and mortality associated with mycoplasmal infection, and social behavior was implicated in disease transmission. The translation of scientific research into management decisions has sometimes led to undesirable outcomes, such as euthanasia of clinically healthy tortoises. In this article, we review and assess current research on tortoise mycoplasmosis, arguably the most important chronic infectious disease of wild and captive North American and European tortoises, and update the implications for management and conservation of tortoises in the wild.

The Veterinary Journal

Globally synchronous ice core volcanic tracers and abrupt cooling during the last glacial period

We perform a Monte Carlo pattern recognition analysis of the coincidence between three regional volcanic histories from ice coring of Greenland and Antarctica over the period 2 to 45 ka, using SO4 anomalies in Greenland and East Antarctica determined by continuous core chemistry, together with West Antarctic volcanic ash layers determined by remote optical borehole logging and core assays. We find that the Antarctic record of volcanism correlates with Glacial abrupt climate change at a 95% to >99.8% (???3??) significance level and that volcanic depositions at the three locations match at levels exceeding 3??, likely indicating that many common horizons represent single eruptive events which dispersed material world wide. These globally coincident volcanics were associated with abrupt cooling, often simultaneous with onsets or sudden intensifications of millennial cold periods. The striking agreement between sites implies that the consistency of current timescales obtained by isotopic and glaciological dating methods is better than estimated. Copyright 2006 by the American Geogphysical Union.

Journal of Geophysical Research D: Atmospheres

Moving beyond p<0.05 in ecotoxicology: A guide for practitioners

Statistical inferences play a critical role in ecotoxicology. Historically, Null Hypothesis Significance Testing (NHST) has been the dominant method for inference in ecotoxicology. As a brief and informal definition of the NHST approach, researchers compare (or test) an experimental treatment or observation against a hypothesis of no relationship or effect (the null hypothesis) using the collected data to see if the observed values are statistically significant given predefined error rates. The resulting probability of observing a value equal to or greater than the observed value assuming the null hypothesis is true is the p-value. Historically, criticisms of NHST have existed for almost a century and more recently these have grown to the point where statisticians, including the American Statistical Association, have felt the need to clarify the role of NHST and p-values in science beyond their current, common use. These limitations also exist in ecotoxicology. For example, a review of the 2010 Environmental Toxicology & Chemistry (ET&C) volume found many authors did not correctly report p-values. We repeated this review looking at the 2019 volume of ET&C and the incorrect reporting of p-values still occurred almost a decade later. Problems with NHST and p-values highlight the need for statistical inferences besides NHST, something that has long been known in ecotoxicology and the broader scientific and statistical communities. Furthermore, concerns such as these led the Executive Director of the American Statistical Association to recommend against use of statistical significance in 2019. In light of these criticisms, however, ecotoxicologists require alternative methods. In this paper, we describe some alternative methods including confidence intervals, regression analysis, dose-response curves, Bayes factors, survival analysis, and model selection. Lastly, we provide insights for what ecotoxicology might look like in a post-p-value world.

Environmental Toxicology and Chemistry

Complementary population dynamics of exotic and native Daphnia in North American reservoir communities

During its invasion of North America, the exotic Daphnia lumholtzi has rapidly colonized numerous reservoirs, natural lakes, and large rivers. In the current study, we examine the overlap between D. lumholtzi and native Daphnia species through analysis of two data sets: co-occurrence in reservoirs of the south-central United States and population dynamics in one reservoir. In 171 reservoirs and oxbow lakes, D. lumholtzi was among the most prevalent species and its distribution was independent of other Daphnia species. Over a 28-month period in Stockton Lake, Missouri, D. lumholtzi was abundant only in late summer, a period when the epilimnion was warm (25-30??C) and cyanobacteria were common. Native Daphnia (D. mendotae, D. parvula, and D. retrocurva) complemented this pattern, being generally rare in summer and most abundant during winter and spring. Peak densities and average fecundities of native Daphnia during the cooler months were typically greater than densities and fecundities of D. lumholtzi in summer. The complementary population dynamics between native and exotic species may be significant for the food webs in warm reservoirs, with D. lumholtzi providing another food resource when the abundance of large zooplankton is ordinarily low. ?? 2006 E. Schweizerbart'sche Verlagsbuchhandlung.

Archiv fur Hydrobiologie

Anatomical and genetic variation of western Oxyloma (Pulmonata: Succineidae) concerning the endangered Kanab ambersnail ( Oxyloma haydeni kanabense ) in Arizona and Utah

The land snail genus Oxyloma (Pulmonata: Succineidae) includes the Federally endangered Kanab ambersnail (Oxyloma haydeni kanabense Pilsbry), which is known at the time of this study from only two locations in the United States: Three Lakes, Utah, and Vaseys Paradise, Arizona, on the Colorado River in Grand Canyon National Park. Since 1994, the Kanab ambersnail has received much attention because its presence at Vaseys Paradise has implications for the ecosystem-wide management of the Colorado River. This attention is primarily because an experimental high-flow release of water from Glen Canyon Dam in 1996 destroyed or degraded Kanab ambersnail habitat at Vaseys Paradise. This experimental high flow was designed to replicate natural flow regimes throughout the Grand Canyon river corridor. However, as a result of the habitat destruction at Vaseys Paradise, in 1996, the U.S. Fish and Wildlife Service ruled that no further experimental high-discharge floods could be carried out until additional Kanab ambersnail populations were discovered or established. This mandate created a situation where the management of a single endangered species conflicted directly with the management of an entire ecosystem. Although since 1996, the U.S. Fish and Wildlife Service has permitted the use of flows as high as stage heights equivalent to 44,000 cubic feet per second, higher flows were requested by various Grand Canyon stakeholders and scientists but were not possible owing to low storage of Lake Powell. Adding to the controversy about Oxyloma and the Kanab ambersnail were previous anatomical and genetic analyses of the genus, which showed that genetic characteristics of specimens did not correspond with their identifications based on traditional taxonomic criteria, raising questions about the validity of the taxonomy of Oxyloma and the protected status of Kanab ambersnails. Specifically, a previous study suggested that the endangered Kanab ambersnail population at Three Lakes was more closely related to other, non-endangered ambersnail populations across the Southwest. In contrast, the Kanab ambersnail population at Vaseys Paradise appeared to be genetically distinct from all other ambersnail populations studied. Management options for the ambersnail population at Vaseys Paradise, at the time of this study, conflict with ecosystem-wide measures proposed to benefit other natural resources in the Grand Canyon. The U.S. Fish and Wildlife Service will not revise the 1995 Kanab Ambersnail Recovery Plan until further genetic and anatomical analyses provide more fine-scale taxonomic resolution of the identity of Oxyloma populations on the Colorado Plateau and elsewhere in the American Southwest. Likewise, interagency cooperators cannot revise down-listing criteria for the Kanab ambersnail until substantial evidence is provided identifying distinct Oxyloma taxa or a larger group of conspecifics that reasonably could be managed as one species. Therefore, given the current controversy about the taxonomy of Oxyloma and the endangered Kanab ambersnail, new detailed analyses were completed of morphological and genetic variation from many Oxyloma specimens collected at 12 western North American locations. These new data have allowed us to evaluate many issues related to Kanab ambersnail taxonomy. Using this dataset, the study of shells and anatomy indicates that the holotype of Oxyloma haydeni kanabense plausibly can be regarded as a member of the same species as the populations of Oxyloma analyzed in this study. Additionally, the presence of gene flow among all populations is evidence that they are members of the same species. Almost all the observed genetic diversity can be accounted for by short-distance or long-distance dispersal events between populations in this study. Our major taxonomic conclusion is that all samples collected for this study were drawn from populations of the same species.

Arizona;Utah

Hydrogeology and trichloroethene contamination in the sea-level aquifer beneath the Logistics Center, Fort Lewis, Washington

The U.S. Army disposed of waste trichloroethene (TCE) and other materials in the East Gate Disposal Yard near the Logistics Center on Fort Lewis, Washington, from the 1940s to the early 1970s. As a result, ground water contaminated with primarily TCE extends more than 3 miles downgradient from the East Gate Disposal Yard. The site is underlain by a complex and heterogeneous sequence of glacial and non-glacial deposits that have been broadly categorized into an upper and a lower aquifer (the latter referred to as the sea-level aquifer). TCE contamination was detected in both aquifers. This report describes an investigation by the U.S. Geological Survey (USGS) of the source, migration, and attenuation of TCE in the sea-level aquifer. A refined conceptual model for ground-water flow and contaminant migration into and through the sea-level aquifer was developed in large part from interpretation of environmental tracer data. The tracers used included stable isotopes of oxygen (18O), hydrogen (2H), and carbon (13C); the radioactive hydrogen isotope tritium (3H); common ions and redox-related analytes; chlorofluorocarbons; and sulfur hexafluoride. Tracer and TCE concentrations were determined for samples collected by the USGS from 37 wells and two surface-water sites in American Lake during 1999-2000. Ground-water levels were measured by the USGS in more than 40 wells during 2000-01, and were combined with measurements by the U.S. Army and others to create potentiometric-surface maps. Localized ground-water flow features were identified that are of particular relevance to the migration of TCE in the study area. A ridge of ground water beneath American Lake diverts the flow of TCE-contaminated ground water in the sea-level aquifer to the west around the southern end of the lake. Tracer data provided clear evidence that American Lake is a significant source of recharge to the sea-level aquifer that has created that ridge of ground water. High ground-water altitudes at locations north and northeast of the Logistics Center combined with the ridge beneath American Lake prevent TCE contaminated water beneath the Logistics Center from migrating toward municipal water-supply wells northeast of the site. The 1999-2000 TCE concentrations measured by the USGS at older wells screened in the sea-level aquifer were similar to those measured since 1995, but the known downgradient extent of the TCE contamination expanded nearly 2 miles after the Army installed and sampled new wells during 2003-04. Concentrations of TCE in the sea-level aquifer were consistently highest in the upper part of the aquifer throughout the plume, although TCE has spread throughout much of the thickness of the aquifer in the downgradient portions of the plume. Environmental tracer data indicated that the primary pathway for contaminant migration into the sea-level aquifer is through the previously identified confining unit window, an area where the predominately fine-grained confining unit is relatively coarse grained and more permeable. Other less substantial pathways for contaminant migration also were identified near the East Gate Disposal Yard and the I-5 pump-and-treat facilities. Those areas are near active pumping wells and ground-water reintroduction facilities, but there is no evidence that the contaminant migration was caused or enhanced by those activities. Within the sea-level aquifer, TCE concentrations continue to migrate westward in the flow field strongly influenced by ground-water recharge from American Lake. Historical data are not available to definitively determine if the 5-?g/L leading edge of the current TCE plume is stable or if it is still moving downgradient. However, an evaluation of the available data combined with TCE traveltime estimates indicates that the peak TCE concentrations in the sea-level aquifer may have not yet reached the wells near the currently defined leading edge of the plume. Hypothetically, the 5-?g/L leading edge

Scientific Investigations Report

Nonlethal screening of bat-wing skin with the use of ultraviolet fluorescence to detect lesions indicative of white-nose syndrome

Definitive diagnosis of the bat disease white-nose syndrome (WNS) requires histologic analysis to identify the cutaneous erosions caused by the fungal pathogen Pseudogymnoascus [formerly Geomyces] destructans (Pd). Gross visual inspection does not distinguish bats with or without WNS, and no nonlethal, on-site, preliminary screening methods are available for WNS in bats. We demonstrate that long-wave ultraviolet (UV) light (wavelength 368&ndash;385 nm) elicits a distinct orange&ndash;yellow fluorescence in bat-wing membranes (skin) that corresponds directly with the fungal cupping erosions in histologic sections of skin that are the current gold standard for diagnosis of WNS. Between March 2009 and April 2012, wing membranes from 168 North American bat carcasses submitted to the U.S. Geological Survey National Wildlife Health Center were examined with the use of both UV light and histology. Comparison of these techniques showed that 98.8% of the bats with foci of orange&ndash;yellow wing fluorescence (n = 80) were WNS-positive based on histologic diagnosis; bat wings that did not fluoresce under UV light (n = 88) were all histologically negative for WNS lesions. Punch biopsy samples as small as 3 mm taken from areas of wing with UV fluorescence were effective for identifying lesions diagnostic for WNS by histopathology. In a nonlethal biopsy-based study of 62 bats sampled (4-mm diameter) in hibernacula of the Czech Republic during 2012, 95.5% of fluorescent (n = 22) and 100% of nonfluorescent (n = 40) wing samples were confirmed by histopathology to be WNS positive and negative, respectively. This evidence supports use of long-wave UV light as a nonlethal and field-applicable method to screen bats for lesions indicative of WNS. Further, UV fluorescence can be used to guide targeted, nonlethal biopsy sampling for follow-up molecular testing, fungal culture analysis, and histologic confirmation of WNS.

Journal of Wildlife Diseases

Changes in body condition and diet of lotic Smallmouth Bass across two flow regimes during summer months at the southern extent of their native range

The Ozark Plateau is located at the southern extent of native Smallmouth Bass Micropterus dolomieu range and water temperature and drought conditions during summer months may potentially affect growth of Smallmouth Bass in this region. Groundwater streams in the region do not warm to the same extent as runoff streams during summer months and could provide a thermal refuge habitat for Smallmouth Bass from high summer temperatures and drought conditions. Our study objective was to examine differences in body condition and diet of Small-mouth Bass through summer months between groundwater and runoff streams. We sampled Smallmouth Bass from eight streams across two the flow regimes monthly from June-September from 2014 to 2016 in the Ozark Plateau of Arkansas and Missouri. Relative weights were calculated and diet contents were examined for each fish. Linear mixed model analyses indicated that relative weights declined in both stream types in 2014 and 2015, but not in 2016. Surprisingly, there was no significant difference in change in relative weights between runoff and groundwater streams in any year. No diet shifts over the course of the summer were noted in any year, and no differences were seen between stream types. Our results suggest that further work should investigate the refuge qualities of groundwater streams for Smallmouth Bass in this region as Smallmouth Bass from both stream types may currently respond similarly to summer conditions.. Middaugh, Chris & Magoulick, Daniel. (2019). Changes in Body Condition and Diet of Lotic Smallmouth Bass across Two Flow Regimes during Summer Months at the Southern Extent of Their Native Range. The Ozark Plateau is located at the southern extent of native Smallmouth Bass Micropterus dolomieu range and water temperature and drought conditions during summer months may potentially affect growth of Smallmouth Bass in this region. Groundwater streams in the region do not warm to the same extent as runoff streams during summer months and could provide a thermal refuge habitat for Smallmouth Bass from high summer temperatures and drought conditions. Our study objective was to examine differences in body condition and diet of Small-mouth Bass through summer months between groundwater and runoff streams. We sampled Smallmouth Bass from eight streams across two the flow regimes monthly from June-September from 2014 to 2016 in the Ozark Plateau of Arkansas and Missouri. Relative weights were calculated and diet contents were examined for each fish. Linear mixed model analyses indicated that relative weights declined in both stream types in 2014 and 2015, but not in 2016. Surprisingly, there was no significant difference in change in relative weights between runoff and groundwater streams in any year. No diet shifts over the course of the summer were noted in any year, and no differences were seen between stream types. Our results suggest that further work should investigate the refuge qualities of groundwater streams for Smallmouth Bass in this region as Smallmouth Bass from both stream types may currently respond similarly to summer conditions.

Arkansas, Missouri

New geologic mapping of the northwestern Willamette Valley, Oregon, and its American Viticultural Areas (AVAs)—A foundation for understanding their terroir

A geologic map of the greater Portland, Oregon, metropolitan area is planned that will document the region’s complex geology (currently in review: “Geologic map of the greater Portland metropolitan area and surrounding region, Oregon and Washington,” by Wells, R.E., Haugerud, R.A., Niem, A., Niem, W., Ma, L., Evarts, R., Madin, I., and others). The map, which is planned to be published as a U.S. Geological Survey Scientific Investigations Map, will consist of 51 7.5′ quadrangles covering more than 2,500 square miles, and it will represent more than 100 person-years of geologic mapping and studies. The region was mapped at the relatively detailed scale of 1:24,000 to improve understanding of its geology and its earthquake hazards. More than 100 geologic map units will record the 50-million-year history of volcanism, sedimentation, folding, and faulting above the Cascadia Subduction Zone. The geology contributes to the varied terroir of four American Viticultural Areas (AVAs) in the northwestern Willamette Valley: the Yamhill-Carlton, Dundee Hills, Chehalem Mountains, and Ribbon Ridge AVAs. Terroir is defined as the environmental conditions, especially climate and soils, that influence the quality and character of a region’s crops—in this case, grapes for wine. On this new poster (“New geologic mapping of the northwestern Willamette Valley, Oregon, and its American Viticultural Areas (AVAs)—A foundation for understanding their terroir”), we present the geologic map at a reduced scale (about 1:175,000) to show the general distribution of geologic map units, and we highlight, discuss, and illustrate six major geologic events that helped shape the region and form its terrior. We also discuss the geologic elements that contribute to the character of each of the four AVAs in the northwestern Willamette Valley.

Oregon