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Field methods, quality-assurance, and data management plan for water-quality activities and water-level measurements, Idaho National Laboratory, Idaho

Introduction Water-quality activities and water-level measurements conducted by the U.S. Geological Survey (USGS) Idaho National Laboratory (INL) Project Office coincide with the USGS mission of evaluating the quantity and quality of the Nation’s water resources. The activities are conducted in cooperation with the U.S. Department of Energy’s (DOE) Idaho Operations Office. Results of water-quality and hydraulic head research efforts are presented in various USGS and scientific journal publications (refer to Fisher, 2022). These data are stored internally in the Aquarius Time Series and Aquarius Samples databases and are publicly accessible through National Water Quality Monitoring Council (2025) and U.S. Geological Survey (2025). Data collected from our studies are used by researchers, Federal and State agencies, water management and regulatory organizations, as well as the public. This quality assurance plan (QAP) describes the methods and processes for field methods, data collection, data management, data auditing, and equipment management for both the water-quality and water-level programs at the USGS INL Project Office (hereto referred to as INL Project Office). A comprehensive quality assurance (QA) plan ensures that the processes defined in this document will guide the program staff to collect and publish reliable, useful, and defensible data products for stakeholders. This QAP supersedes previous versions of this document and is intended to complement the Quality Assurance and Data Management (QADM) Plan for the Idaho Water Science Center (IDWSC; Christopher Mebane and Lauren Zinsser, written commun., 2024).

Idaho↗

Precipitation-runoff modeling system; user's manual

The concepts, structure, theoretical development, and data requirements of the precipitation-runoff modeling system (PRMS) are described. The precipitation-runoff modeling system is a modular-design, deterministic, distributed-parameter modeling system developed to evaluate the impacts of various combinations of precipitation, climate, and land use on streamflow, sediment yields, and general basin hydrology. Basin response to normal and extreme rainfall and snowmelt can be simulated to evaluate changes in water balance relationships, flow regimes, flood peaks and volumes, soil-water relationships, sediment yields, and groundwater recharge. Parameter-optimization and sensitivity analysis capabilites are provided to fit selected model parameters and evaluate their individual and joint effects on model output. The modular design provides a flexible framework for continued model system enhancement and hydrologic modeling research and development. (Author 's abstract)

Water-Resources Investigations Report↗

Water velocity regulates macro-consumer herbivory on the benthic macrophyte Podostemum ceratophyllum Michx.

1) Macrophytes influence aquatic ecosystems by increasing habitat complexity and providing trophic resources for aquatic fauna. While herbivory on freshwater macrophytes is widely documented in lakes, low-velocity riverine habitats, the influence of herbivory on macrophytes in higher-velocity habitats has rarely been examined. 2) We investigated the hypothesis that high water velocity can reduce consumption rates of the submerged macrophyte, Podostemum ceratophyllum, an angiosperm which grows attached to stable substrates in high water-velocity riverine habitats throughout eastern North America. 3) We estimated grazing-pressure by conducting short-term (up to 77-d) transplant and consumer-exclosure experiments, and quantified changes in Podostemum stem length when the plant was eposed to low (<0.5 m s-1) and higher velocities in two Piedmont rivers in Georgia (USA). Podostemum transplanted into low-velocity habitats rapidly lost stem length unless macro-consumers were excluded from accessing the plant. In contrast, Podostemum tranplanted into high-velocity habitats showed little change or gained stem length. 4) We estimated that 85% (67 – 98%; 95% credible interval) of the daily stem growth (0.026 cm cm-1 day-1) in Podostemum was consumed during a 77-d paired consumer-access versus exclosure experiment conducted in mean water velocities of 0.35-0.5 m s-1. We also found a positive relation (R2 = 0.58) between Podostemum biomass and water velocity (ranging between ~0.1 -1.4 m s-1) in benthic samples collected over a two-month period. 5) We conclude that high water velocity reduces herbivory on Podostemum, and that water velocity can thus control the acural of benthic plant biomass and the movement of plant-derived materials through benthic food webs. Our research has implications for estimating resource storage and flux in lotic food webs and illuminates a mechanism by which flow regulation and management may affect basal resources in rivers.

Georgia↗

Processes influencing the transport and fate of contaminated sediments in the coastal ocean– Boston Harbor and Massachusetts Bay

Most of the major urban centers of the United States including Boston, New York, Washington, Chicago, New Orleans, Miami, Los Angeles, San Francisco, and Seattle—are on a coast (fig. 1.1). All of these cities discharge treated sewage effluent into adjacent waters. In 2000, 74 percent of the U.S. population lived within 200 kilometers (km) of the coast. Between 1980 and 2002, the population density in coastal communities increased approximately 4.5 times faster than in noncoastal areas of the U.S. (Perkins, 2004). More people generate larger volumes of wastes, increase the demands on wastewater treatment, expand the area of impervious land surfaces, and use more vehicles that contribute contaminants to street runoff. According to the National Coastal Condition Report II (U.S. Environmental Protection Agency, 2005a), on the basis of coastal habitat, water and sediment quality, benthic index, and fish tissue, the overall national coastal condition is only poor to fair and the overall coastal condition in the highly populated Northeast is poor. Scientific information helps managers to prioritize and regulate coastal-ocean uses that include recreation, commercial fishing, transportation, waste disposal, and critical habitat for marine organisms. These uses are often in conflict with each other and with environmental concerns. Developing a strategy for managing competing uses while maintaining sustainability of coastal resources requires scientific understanding of how the coastal ocean system behaves and how it responds to anthropogenic influences. This report provides a summary of a multidisciplinary research program designed to improve our understanding of the transport and fate of contaminants in Massachusetts coastal waters. Massachusetts Bay and Boston Harbor have been a focus of U.S. Geological Survey (USGS) research because they provide a diverse geographic setting for developing a scientific understanding of the geology, geochemistry, and oceanography of coastal systems in general. Scientific data from this region can also be used to inform decisions about important economic, environmental, and political issues. From the economic viewpoint, the annual value of tourism and shipping in Massachusetts and Cape Cod Bays is about $1.5 billion and $1.9 billion, respectively. Commercial and recreational fishing generates about $240 million per year in the same region (U.S. Environmental Protection Agency, 2005b). The environmental issue is the 300-year history of waste discharge from the Boston metropolitan area into the harbor. This history is punctuated by cycles of environmental degradation, public outcry, and improvements in the sewage treatment system. With each improvement, however, the continuous growth of population in greater Boston (fig. 1.2) and the resulting increase in the volume of waste exceeded the capacity of the treatment system, thereby setting the stage for a new contamination crisis. By the 1980s, the levels of contaminants in sediments of Boston Harbor were among the highest in the nation (National Oceanic and Atmospheric Administration, 1987). Fish were diseased, shellfish beds were closed, and swimming beaches were unsafe after heavy rains; in general, water quality and aesthetics were below acceptable standards. Legal and political issues have always been part of Boston Harbor’s history. The environmental conditions in the 1980s were highlighted in a 1983 legal suit brought by the city of Quincy against the Metropolitan District Commission (MDC, the state agency responsible for sewage treatment) and heads of three state agencies for discharging untreated or poorly treated sewage into the harbor (Dolin, 2004). The suit never went to trial, but through the actions of a Massachusetts Superior Court, the issue of Boston Harbor contamination remained on the political and public agenda. The judge called the harbor “unsafe, unsanitary, indecent, in violation of the law (Clean Water Act), and a danger to the health and welfare of the people” (Forman, 1984). To force the state legislature to implement a plan to improve harbor conditions, the judge threatened to place the MDC in receivership and curtail new sewage hookups for industry. Under intense lobbying by business, the legislature created the Massachusetts Water Resources Authority (MWRA) in December 1984. The independent MWRA was established to manage Boston’s waste treatment system and was given the authority to float bonds to pay for major improvements in the treatment system. In 1985, a Federal court began hearings on a suit brought by the Conservation Law Foundation, the Environmental Protection Agency (USEPA), and towns of Quincy and Winthrop against the MDC and MWRA (as heir to responsibilities of the MDC) for years of violation of the Clean Water Act. The judge ruled against the defendants and required all the parties to submit a construction plan and schedule for a new sewage treatment system. From these submissions, he developed a schedule for treatment system upgrades that would give the “citizens of this commonwealth a public assurance that Boston Harbor will be cleaned up within a defined period of time” (Dolin, 2004). The MWRA’s Boston Harbor cleanup program (Levy and Connor, 1992) has transformed the Boston sewage system. Key improvements were to (1) reduce contaminants at the industrial source; (2) remediate leaks in the sewage-collection system; (3) eliminate sewage sludge discharge to the harbor; (4) upgrade sewage treatment from primary to secondary; (5) construct a new ocean outfall 15.2 km offshore in Massachusetts Bay for discharge of treated effluent (fig. 1.3); and (6) implement improvements in the combined-sewer-overflow system. As part of the harbor cleanup program, the MWRA developed a comprehensive monitoring program (summarized in MWRA, 2004) to assess changes in the harbor and bays that specifically related to the new sewage system. Additional information about conditions and processes in the coastal system on a regional scale and over a long time period was and continues to be important in predicting and interpreting local change. Implementation of the MWRA’s program and the mission of the USGS to understand the geology of the nation’s offshore waters provided an opportunity to conduct a cooperative multidisciplinary research program. This USGS program addresses basic scientific questions as well as concerns raised by management regarding the design, implementation, and assessment of the new sewage treatment system. Already active in Boston Harbor during the late 1970s, the USGS expanded research into Massachusetts Bay with a multidisciplinary program in 1989.

Massachusetts↗

Challenges for creating a site-specific groundwater-use record for the Ozark Plateaus aquifer system (central USA) from 1900 to 2010

Hydrologic budgets to determine groundwater availability are important tools for water-resource managers. One challenging component for developing hydrologic budgets is quantifying water use through time because historical and site-specific water-use data can be sparse or poorly documented. This research developed a groundwater-use record for the Ozark Plateaus aquifer system (central USA) from 1900 to 2010 that related county-level aggregated water-use data to site-specific well locations and aquifer units. A simple population-based linear model, constrained to 0 million liters per day in 1900, provided the best means to extrapolate groundwater-withdrawal rates pre-1950s when there was a paucity of water-use data. To disaggregate county-level data to individual wells across a regional aquifer system, a programmatic hierarchical process was developed, based on the level of confidence that a well pumped groundwater for a specific use during a specific year. Statistical models tested on a subset of the best-available site-specific water-use data provided a mechanism to bracket historic groundwater use, such that groundwater-withdrawal rates ranged, on average, plus or minus 38% from modeled values. Groundwater withdrawn for public supply and domestic use accounted for between 48 and 74% of total groundwater use since 1901, highlighting that groundwater provides an important drinking-water resource. The compilation, analysis, and spatial and temporal extrapolation of water-use data remain a challenging task for water scientists, but is of paramount importance to better quantify groundwater use and availability.

Arkansas, Kansas, Missouri, Oklahoma↗

Application of the technology readiness levels framework to natural resource management tools

Technology advancements in fisheries science can provide useful tools to support natural resource management and conservation. However, new technologies may also present challenges for decision makers due to the lack of a standardized process to assess technologies for consideration within management plans. Future technology development in fishery and water resources could benefit from a framework that assigns an appropriate technology development stage and defines the readiness for implementation. Technology Readiness Levels (TRL) are one established research and development scale used throughout engineering and related disciplines that could be applied to natural resource management tools. The TRL assess the maturity of a technology from nascent idea through a fully developed technology. Steps within this scale could provide a general framework for researchers to follow when planning and conducting studies, while similarly providing a standard scale for resource managers to assess readiness for technology transfer and implementation. The goal of this paper is to describe TRL in the context of natural resource management tools and offer this existing framework as one option to facilitate communication between researchers and managers.

Fisheries↗

Detroit River becoming a crucible for boundary organization experimentation

The Detroit River has a long history of human use and abuse, resulting in public outcry over water pollution and resource degradation. This public outcry helped catalyze the enactment of many laws and the Canada-U.S. Great Lakes Water Quality Agreement which led to enhanced research, monitoring, and water pollution control. As pollution from industries and municipal wastewater treatment plants came under control and progress was made in management of single species, the focus shifted to a more comprehensive ecosystem approach that accounted for all the sources of pollution and targeted restoring ecosystem health with resilience. Over time, the Detroit River became a “proving ground” or crucible for experimenting with boundary organizations to overcome geographical, political, institutional, and disciplinary boundaries and strengthen science-policy-management linkages for ecosystem-based management. This study identified 15 boundary organizations functioning in the Detroit River watershed and evaluated two case studies – St. Clair-Detroit River System Initiative and State of the Strait Conferences. Key lessons learned from this study include: 1) establishing boundary organizations, promoting cooperative learning, and building capacity for boundary-spanning are essential for use of an ecosystem approach; 2) boundary spanning requires specific skills, experience, and improved linkages between research and practice; 3) the 15 boundary organizations provide a unique opportunity to collaborate in a community of practice to share knowledge, foster cooperative learning, enhance problem-solving, build trust, and demonstrate leadership; and 4) continued actionable science, investment in capacity building, and cooperative learning are essential to meet long-term goals of sustainability.

Detroit River↗

Summer stream water temperature models for Great Lakes streams: New York

Temperature is one of the most important environmental influences on aquatic organisms. It is a primary driver of physiological rates and many abiotic processes. However, despite extensive research and measurements, synoptic estimates of water temperature are not available for most regions, limiting our ability to make systemwide and large-scale assessments of aquatic resources or estimates of aquatic species abundance and biodiversity. We used subwatershed averaging of point temperature measurements and associated multiscale landscape habitat conditions from over 3,300 lotic sites throughout New York State to develop and train artificial neural network models. Separate models predicting water temperature (in cold, cool, and warm temperature classes) within small catchment–stream order groups were developed for four modeling units, which together encompassed the entire state. Water temperature predictions were then made for each stream segment in the state. All models explained more than 90% of data variation. Elevation, riparian forest cover, landscape slope, and growing degree-days were among the most important model predictors of water temperature classes. Geological influences varied among regions. Predicted temperature distributions within stream networks displayed patterns of generally increasing temperature downstream but were patchy due to the averaging of water temperatures within stream size-classes of small drainages. Models predicted coldwater streams to be most numerous and warmwater streams to be generally associated with the largest rivers and relatively flat agricultural areas and urban areas. Model predictions provide a complete, georeferenced map of summer daytime mean stream temperature potential throughout New York State that can be used for planning and assessment at spatial scales from the stream segment class to the entire state.

New York↗

Assessing sandhill crane roosting habitatalong the Platte River, Nebraska

Each spring approximately 500,000 sandhill cranes and some endangered whooping cranes use the Central Platte River Valley in Nebraska as a staging habitat during their migration north to breeding and nesting grounds in Canada, Alaska, and the Siberian Arctic. Over the last century changes in the flow of the river have altered the river channels and the distribution of roost sites. USGS researchers studied linkages between water flow, sediment supply, channel morphology, and preferred sites for crane roosting. These results are useful for estimating crane populations and for providing resource managers with techniques to understand crane habitats.

Fact Sheet↗

Overview of the National Water-Quality Assessment Program

The Nation's water resources are the basis for life and our economic vitality. These resources support a complex web of human activities and fishery and wildlife needs that depend upon clean water. Demands for good-quality water for drinking, recreation, farming, and industry are rising, and as a result, the American public is concerned about the condition and sustainability of our water resources. The American public is asking: Is it safe to swim in and drink water from our rivers or lakes? Can we eat the fish that come from them? Is our ground water polluted? Is water quality degrading with time, and if so, why? Has all the money we've spent to clean up our waters, done any good? The U.S. Geological Survey's National Water-Quality Assessment (NAWQA) Program was designed to provide information that will help answer these questions. NAWQA is designed to assess historical, current, and future water-quality conditions in representative river basins and aquifers nationwide. One of the primary objectives of the program is to describe relations between natural factors, human activities, and water-quality conditions and to define those factors that most affect water quality in different parts of the Nation. The linkage of water quality to environmental processes is of fundamental importance to water-resource managers, planners, and policy makers. It provides a strong and unbiased basis for better decisionmaking by those responsible for making decisions that affect our water resources, including the United States Congress, Federal, State, and local agencies, environmental groups, and industry. Information from the NAWQA Program also will be useful for guiding research, monitoring, and regulatory activities in cost effective ways.

Open-File Report↗

U.S. Geological Survey (USGS) Water-Use Data and Research (WUDR) program overview and status as of March 31, 2022

The USGS Water-Use Data and Research Program (WUDR) is an appropriated program and is authorized under the SECURE Water Act (Sec. 9508 (c)). WUDR provides financial assistance through cooperative agreements to State water resource agencies. The WUDR Program has two main goals: • To improve the availability, quality, compatibility, and delivery of water-use data that are collected and/or estimated by States to support national water-use assessments; and • To integrate the water-use data into USGS databases in electronic or machine-readable formats.

Newsletter↗

Fort Collins Science Center: science accomplishments for fiscal years 2012 and 2013

The Fort Collins Science Center (FORT) is a multi-disciplinary research and development center of the U.S. Geological Survey (USGS) located in Fort Collins, Colorado. Organizationally, FORT is within the USGS Southwest Region, although our work extends across the Nation and into several other countries. FORT research focuses on needs of the land- and water-management bureaus within the U.S. Department of the Interior (DOI), other Federal agencies, and those of State and non-government organizations. As a Science Center, we emphasize a multi-disciplinary science approach to provide information for resource-management decisionmaking. FORT’s vision is to maintain and continuously improve the integrated, collaborative, world-class research needed to inform effective, science-based land and resource management. Our science and technological development activities and unique capabilities support all USGS scientific Mission Areas and contribute to successful, collaborative science efforts across the USGS and DOI. We organized our report into an Executive Summary, a cross-reference table, and an appendix. The executive summary provides brief highlights of some key FORT accomplishments for each Mission Area. The table cross-references all major FY2012 and FY2013 science accomplishments with the various Mission Areas that each supports. The one-page accomplishment descriptions in the appendix are organized by USGS Mission Area and describe the many and diverse ways in which our science is applied to resource issues. As in prior years, lists of all FY2012 and FY2013 publications and other product types also are appended.

Open-File Report↗

Agriculture — A river runs through it — The connections between agriculture and water quality

Sustaining the quality of the Nation’s water resources and the health of our diverse ecosystems depends on the availability of sound water-resources data and information to develop effective, science-based policies. Effective management of water resources also brings more certainty and efficiency to important economic sectors. Taken together, these actions lead to immediate and longterm economic, social, and environmental benefits that make a difference to the lives of the almost 400 million people projected to live in the United States by 2050. In 1991, Congress established the U.S. Geological Survey National Water-Quality Assessment (NAWQA) to address where, when, why, and how the Nation’s water quality has changed, or is likely to change in the future, in response to human activities and natural factors. Since then, NAWQA has been a leading source of scientific data and knowledge used by national, regional, state, and local agencies to develop science-based policies and management strategies to improve and protect water resources used for drinking water, recreation, irrigation, energy development, and ecosystem needs. Plans for the third decade of NAWQA (2013–23) address priority water-quality issues and science needs identified by NAWQA stakeholders, such as the Advisory Committee on Water Information and the National Research Council, and are designed to meet increasing challenges related to population growth, increasing needs for clean water, and changing land-use and weather patterns. This report is one of a series of publications, The Quality of Our Nation’s Waters , which describes major findings of the NAWQA Project on water-quality issues of regional and national concern and provides science-based information for assessing and managing the quality of our groundwater resources. Other reports in this series focus on occurrence and distribution of nutrients, pesticides, and volatile organic compounds in streams and groundwater, the effects of contaminants and stream-flow alteration on the condition of aquatic communities in streams, and on the quality of groundwater from private domestic and public supply wells. Each reports builds toward a more comprehensive understanding of the quality of regional and national water resources. All NAWQA reports are available online ( https://water.usgs.gov/nawqa/bib/ ). We hope this publication will provide you with insights and information to meet your water-resource needs and will foster increased citizen awareness and involvement in the protection and restoration of our Nation’s waters. The information in this report is intended primarily for those interested or involved in resource management and protection, conservation, regulation, and policymaking at the regional and national levels.

Circular↗

Prioritization framework for ranking riverine ecosystem stressors using example sites from the Tualatin River Basin, Oregon

As human populations increase, so does their influence over the environment. Altered terrain, degraded water quality, and threatened or endangered species are all-too-common consequences of a growing anthropogenic influence on the landscape. To help manage these effects, researchers have developed new ways to characterize current environmental conditions and help resource managers seek solutions to bring affected areas back to their best attainable health. Before an ecosystem can be improved, however, its current level of ecological stress must be determined. Characterizing environmental conditions at many sites across a landscape helps managers understand the range of current conditions and prioritize where they might focus restoration and protection efforts. This report details the development of a prioritization framework to score riverine ecosystem stressors in a watershed based on example sites from the Tualatin River Basin in northwestern Oregon. The framework incorporated the most influential site-specific stressors throughout the basin built on a long history of data collection. These stressors were characterized with 13 metrics that were organized into 4 groups: hydrologic, water quality, physical habitat, and biological. Each stressor metric used readily accessible data and was translated to a score between 0 and 10. The higher the score, the healthier the site. This initial application of the framework used field observations and measurements to rank site conditions at two Tualatin River sites and four Tualatin River tributary sites. Given the versatility of this framework, it easily could be expanded to include more sites or new metrics, if necessary. Because stressors varied by season, all metrics for the tributary sites were scored separately during the wet season (November through April) and dry season (May through October). Water-quality data were available over a prolonged period; therefore, water-quality metrics were assessed by season and by decade (1990–99 compared to 2000–12) to evaluate long-term stressor trends. Results for the Tualatin River Basin prioritization framework indicated that the urban tributaries demonstrated the greatest stress throughout the year, especially during the dry summer months. Spatially, the upper Tualatin River was healthier than the lower reaches of the river. Water-quality has improved in the last 10 years, mostly due to improvements in the dry period contaminant scores, but challenges remain with high water temperatures and low dissolved-oxygen conditions. The biggest challenge with this type of research derived from inconsistencies within the available data. Both spatial and temporal data gaps must be addressed to improve the prioritization. Incorporating both discrete and continuous datasets into the prioritization framework remains a challenge because the datasets have slightly different information and criteria and are not always comparable. Regardless, this report provides guidelines for developing a prioritization framework that ranks the ecological health of sites in a watershed and provides guidance on management actions for improving conditions by targeting factors that greatly affect the health of river ecosystems.

Oregon↗

Geospatial decision support systems for societal decision making

While science provides reliable information to describe and understand the earth and its natural processes, it can contribute more. There are many important societal issues in which scientific information can play a critical role. Science can add greatly to policy and management decisions to minimize loss of life and property from natural and man-made disasters, to manage water, biological, energy, and mineral resources, and in general, to enhance and protect our quality of life. However, the link between science and decision-making is often complicated and imperfect. Technical language and methods surround scientific research and the dissemination of its results. Scientific investigations often are conducted under different conditions, with different spatial boundaries, and in different timeframes than those needed to support specific policy and societal decisions. Uncertainty is not uniformly reported in scientific investigations. If society does not know that data exist, what the data mean, where to use the data, or how to include uncertainty when a decision has to be made, then science gets left out -or misused- in a decision making process. This paper is about using Geospatial Decision Support Systems (GDSS) for quantitative policy analysis. Integrated natural -social science methods and tools in a Geographic Information System that respond to decision-making needs can be used to close the gap between science and society. The GDSS has been developed so that nonscientists can pose "what if" scenarios to evaluate hypothetical outcomes of policy and management choices. In this approach decision makers can evaluate the financial and geographic distribution of potential policy options and their societal implications. Actions, based on scientific information, can be taken to mitigate hazards, protect our air and water quality, preserve the planet's biodiversity, promote balanced land use planning, and judiciously exploit natural resources. Applications using the GDSS have demonstrated the benefits of utilizing science for policy decisions. Investment in science reduces decision-making uncertainty and reducing that uncertainty has economic value.

Boletin Geologico y Minero↗

How low is too low? Partnering with stakeholders and managers to define ecologically based low-flow thresholds in a perennial temperate river

Managing aquatic ecosystems for people and nature can be improved by collaboration among scientists, managers, decision-makers, and other stakeholders. Many collaborative and interdisciplinary approaches have been developed to address the management of freshwater ecosystems; however, there are still barriers to overcome. We worked as part of a regional stakeholder group comprising municipal water utility operators, conservation organizations, academic partners, and other stakeholders to understand the effects of low-flow and drought on ecological functions of the upper Flint River, Georgia (USA), a free-flowing river important for municipal water supply, recreation, and native biota. We used published literature and locally targeted studies to identify quantitative flow targets that could be used to inform water management and drought planning. Drawing from principles of Translational Ecology, we relied on an iterative process to develop information needs for the group and maintained communication and engagement throughout data collection, analysis, and synthesis. We identified three quantitative flow benchmarks to evaluate the ecological impacts of drought in the river. The results were valuable to both the water utilities represented in the working group and State regional water planning, which is used to guide water management strategies and permitting for the basin. We identified principles that were important for the successful engagement in the working group and helped to overcome the challenge of working across sectors and without direct authority guiding the implementation of our work. Interdisciplinary work and creative solutions are crucial to plan for and adapt to greater pressure on our water resources.

River Research and Applications↗

Comparison of two cell lysis procedures for recovery of microcystins in water samples from silver lake in Dover, Delaware, with microcystin producing cyanobacterial accumulations

A collaboration was developed between Abraxis, LLC, the State of Delaware Department of Natural Resources and Environmental Control Division of Water Resources Environmental Laboratory, the University of Delaware, and the United States Geological Survey to investigate the efficacy of the QuikLyse procedure developed by Abraxis, LLC as an alternative cell-lysis technique suitable for use with an existing liquid chromatography/tandem mass spectrometry research method developed at the United States Geological Survey Organic Geochemistry Research Laboratory to analyze cyanotoxins. A comparison of three sequential freeze/thaw cycles versus QuikLyse, a proprietary chemical lysis procedure was conducted on four water samples collected from Silver Lake in Dover, Delaware. Results from the Abraxis Microcystins-DM enzyme-linked immunosorbent assay and liquid chromatography/tandem mass spectrometry were tabulated as a function of the cell lysis technique. Stastical comparison of percent relative standard deviations showed no significant difference (alpha = 0.05) between both cell-lysis techniques when measured by enzyme-linked immunosorbent assay or liquid chromatography/tandem mass spectrometry for three of the four samples.

Delaware↗