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Development and assessment of fluorescent-dyed, preserved invasive grass carp (Ctenopharyngodon idella) eggs as surrogates for live eggs in transport and dispersal control experiments

Invasive species such as grass carp ( Ctenopharyngodon idella ) pose substantial ecological threats to North American freshwater ecosystems. Understanding their early life stage behavior is critical for management efforts. From spawning to hatching, invasive carp eggs must remain suspended in the water column while drifting downstream for the best chance of survival. This highly vulnerable life stage is a potential target for population control to reduce recruitment. However, studying egg transport and potential dispersal control techniques is challenging, because the availability of live eggs and time period for experimentation are extremely limited. Additionally, accurately replicating the physical characteristics and transport mechanisms of fish eggs using surrogates in laboratory and field studies is not trivial. This study presents a novel method to create fluorescein-dyed, preserved grass carp eggs as surrogates for live eggs in transport and dispersal control experiments. This technique enables year-round studies of grass carp egg transport, offering managers a reliable tool for developing and testing dispersal control and passive sampling methods for invasive carp eggs. In this study, we rehydrate and dye preserved grass carp eggs in varying concentrations of aqueous fluorescein for a range of rehydration times, evaluate dye retention and egg visibility under ultraviolet light (UV-A), and measure diameters and settling velocities for comparison with live eggs. Eggs rehydrated in 0.100 g per liter fluorescein for 30 min maintain adequate brightness for up to 40 min in mixed conditions and exhibit mean settling velocities and densities similar to live eggs, making them ideal for laboratory experiments using quantitative imaging techniques.

River Research and Applications

Fission-track and K-Ar ages of Tertiary ash-flow tuffs, north-central Nevada

Ages obtained from three Tertiary ash-flow tuffs in central Nevada by fission-track and K-Ar dating are concordant. Samples dated by these methods from the same localities give the same age within the limits of analytical uncertainty. Samples of three units from widely separated localities were dated further to confirm the concordance of the dating methods and to establish that ages can be used, in conjunction with normal geologic techniques, as a criteria for correlation. The minerals dated by K-Ar were biotite and sanidine; and sphene zircon and apatite were used to determine the fission track ages. The Bates Mountain Tuff was dated at 24.0 m.y. (F.T.) and 24.0 m.y. (K-Ar). The Fish Creek Mountains Tuff has a fission-track age of 24.4 m.y., and a K-Ar age of 23.9 m.y. The average fission-track age for the Caetano Tuff is 34.9 m.y., and it has an average K-Ar age of 32.3 m.y. The amount of analytical uncertainty is slightly greater in the fission track method. Sampling, preparation, and determination of the age by the fission-track method is competitive with the K-Ar method.

Nevada

Surface-Water Techniques: On Demand Training Opportunities

The U.S. Geological Survey (USGS) has been collecting streamflow information since 1889 using nationally consistent methods. The need for such information was envisioned by John Wesley Powell as a key component for settlement of the arid western United States. Because of Powell?s vision the nation now has a rich streamflow data base that can be analyzed with confidence in both space and time. This means that data collected at a stream gaging station in Maine in 1903 can be compared to data collected in 2007 at the same gage in Maine or at a different gage in California. Such comparisons are becoming increasingly important as we work to assess climate variability and anthropogenic effects on streamflow. Training employees in proper and consistent techniques to collect and analyze streamflow data forms a cornerstone for maintaining the integrity of this rich data base.

Fact Sheet

lsforce: A Python-based single-force seismic inversion framework for massive landslides

We present an open‐source Python package, lsforce, for performing single‐force source inversions of long‐period (tens to hundreds of seconds) seismic signals. Although the software is designed primarily for landslides, it can be used for any single‐force seismic source. The package allows users to produce estimates of the three‐component time series of forces exerted on the Earth by a landslide with postprocessing options to estimate the trajectory of its center of mass. Green’s functions for a user‐selected 1D Earth model are obtained automatically from the Incorporated Research Institutions for Seismology Synthetics Engine webservice or can be computed for custom 1D Earth models using Computer Programs in Seismology. lsforce implements the two most commonly used source parameterizations: a fully flexible, high‐resolution approach and a more stable but lower‐resolution method of overlapping triangle sources. Regularization options include a blended zeroth‐, first‐, and second‐order semiautomated Tikhonov regularization scheme, as well as additional optional constraints on start times, end times, and on the sum of forces. Uncertainty due to data selection can be assessed using either a leave‐one‐out approach or a modified jackknife technique that randomly excludes subsets of the data for multiple re‐inversions. Numerous built‐in plotting methods allow for easy quality control and assessment of results. In this article, we briefly outline the theory and methodology, describe our implementation, and demonstrate the usage of lsforce using the well‐studied 28 June 2016 Lamplugh rock avalanche in Alaska. Despite the rapidly increasing prevalence of landslide single‐force inversions in the landslide and seismology literature over the past decade, to our knowledge this is the first open‐source code for performing such inversions.

Seismological Research Letters

Field and laboratory determination of water-surface elevation and velocity using noncontact measurements

Noncontact methods for measuring water-surface elevation and velocity in laboratory flumes and rivers are presented with examples. Water-surface elevations are measured using an array of acoustic transducers in the laboratory and using laser scanning in field situations. Water-surface velocities are based on using particle image velocimetry or other machine vision techniques on infrared video of the water surface. Using spatial and temporal averaging, results from these methods provide information that can be used to develop estimates of discharge for flows over known bathymetry. Making such estimates requires relating water-surface velocities to vertically averaged velocities; the methods here use standard relations. To examine where these relations break down, laboratory data for flows over simple bumps of three amplitudes are evaluated. As anticipated, discharges determined from surface information can have large errors where nonhydrostatic effects are large. In addition to investigating and characterizing this potential error in estimating discharge, a simple method for correction of the issue is presented. With a simple correction based on bed gradient along the flow direction, remotely sensed estimates of discharge appear to be viable.

Conference Paper

High-resolution Monte Carlo simulation of flow and conservative transport in heterogeneous porous media: 2. Transport results

In this, the second of two papers concerned with the use of numerical simulation to examine flow and transport parameters in heterogeneous porous media via Monte Carlo methods, results from the transport aspect of these simulations are reported on. Transport simulations contained herein assume a finite pulse input of conservative tracer, and the numerical technique endeavors to realistically simulate tracer spreading as the cloud moves through a heterogeneous medium. Medium heterogeneity is limited to the hydraulic conductivity field, and generation of this field assumes that the hydraulic-conductivity process is second-order stationary. Methods of estimating cloud moments, and the interpretation of these moments, are discussed. Techniques for estimation of large-time macrodispersivities from cloud second-moment data, and for the approximation of the standard errors associated with these macrodispersivities, are also presented. These moment and macrodispersivity estimation techniques were applied to tracer clouds resulting from transport scenarios generated by specific Monte Carlo simulations. Where feasible, moments and macrodispersivities resulting from the Monte Carlo simulations are compared with first- and second-order perturbation analyses. Some limited results concerning the possible ergodic nature of these simulations, and the presence of non-Gaussian behavior of the mean cloud, are reported on as well.

Water Resources Research

Lithium metaborate flux in silicate analysis

Lithium metaborate is an effective flux for silicates and other rock-forming minerals. The glass resulting from fusion is mechanically strong, reasonably nonhygroscopic, and is readily soluble in dilute acids. These characteristics lead to its use in X-ray spectrography and in methods which require whole-rock solutions, such as atomic absorption and emission spectrometry. Difficulties have been encountered in the use of such techniques : a high-quality reagent has been difficult to obtain ; fusion conditions must be rather closely controlled; graphite crucibles used in the fusions need special treatment. Methods for overcoming these difficulties are outlined. Selected procedures for various instrumental methods of analysis are described.

Analytica Chimica Acta

A comparison of four porewater sampling methods for metal mixtures and dissolved organic carbon and the implications for sediment toxicity evaluations

Evaluations of sediment quality conditions are commonly conducted using whole-sediment chemistry analyses but can be enhanced by evaluating multiple lines of evidence, including measures of the bioavailable forms of contaminants. In particular, porewater chemistry data provide information that is directly relevant for interpreting sediment toxicity data. Various methods for sampling porewater for trace metals and dissolved organic carbon (DOC), which is an important moderator of metal bioavailability, have been employed. The present study compares the peeper, push point, centrifugation, and diffusive gradients in thin films (DGT) methods for the quantification of 6 metals and DOC. The methods were evaluated at low and high concentrations of metals in 3 sediments having different concentrations of total organic carbon and acid volatile sulfide and different particle-size distributions. At low metal concentrations, centrifugation and push point sampling resulted in up to 100 times higher concentrations of metals and DOC in porewater compared with peepers and DGTs. At elevated metal levels, the measured concentrations were in better agreement among the 4 sampling techniques. The results indicate that there can be marked differences among operationally different porewater sampling methods, and it is unclear if there is a definitive best method for sampling metals and DOC in porewater.

Environmental Toxicology and Chemistry

Pilot testing and protocol development of giant applesnail suppression at Mandalay National Wildlife Refuge, Louisiana—July–October 2017

This report provides an overview of the pilot study and description of the techniques developed for a future mitigation study of Pomacea maculata (giant applesnail) at the U.S. Fish and Wildlife Service Mandalay National Wildlife Refuge, Louisiana (MNWR). Egg mass suppression is a potential strategy for the mitigation of the invasive giant applesnail. In previous studies at Langan Municipal Park in Mobile, Alabama (LMP), and National Park Service Jean Lafitte National Park-Barataria Unit, Louisiana (JLNP), we determined that spraying food-grade oil (coconut oil or Pam™ spray) on egg masses significantly reduced egg hatching. At JLNP we also developed methods to estimate snail population size. The purpose of this pilot study was to adapt techniques developed for previous studies to the circumstances of MNWR in preparation for a larger experiment whereby we will test the effectiveness of egg mass suppression as an applesnail mitigation tool. We selected four canals that will be used as treatment and control sites for the experiment (two each). We established that an efficient way to destroy egg masses is to knock them down with a high-velocity stream of water pumped directly from the canal. The traps used at JLNP had to be modified to accommodate the greater range of water-level fluctuation at MNWR. One of the three marking methods used at JLNP was selected for use at MNWR.

Louisiana

Analysis and simulation of water-level, specific conductance, and total phosphorus dynamics of the Loxahatchee National Wildlife Refuge, Florida, 1995-2006

The Arthur R. Marshall Loxahatchee Wildlife Refuge (Refuge) was established in 1951 through a license agreement between the South Florida Water Management District and the U.S. Fish and Wildlife Service (USFWS) as part of the Migratory Bird Conservation Act. Under the license agreement, the State of Florida owns the land of the Refuge and the USFWS manages the land. Fifty-seven miles of levees and borrow canals surround the Refuge. Water in the canals surrounding the marsh is controlled by inflows and outflows through control structures. The transport of canal water with higher specific conductance and nutrient concentrations to the interior marsh has the potential to alter critical ecosystem functions of the marsh. Data-mining techniques were applied to 12 years (1995-2006) of historical data to systematically synthesize and analyze the dataset to enhance the understanding of the hydrology and water quality of the Refuge. From the analysis, empirical models, including artificial neural network (ANN) models, were developed to answer critical questions related to the relative effects of controlled releases, precipitation, and meteorological forcing on water levels, specific conductance, and phosphorous concentrations of the interior marsh. Data mining is a powerful tool for converting large databases into information to solve complex problems resulting from large numbers of explanatory variables or poorly understood process physics. For the application of the linear regression and ANN models to the Refuge, data-mining methods were applied to maximize the information content in the raw data. Signal processing techniques used in the data analysis and model development included signal decomposition, digital filtering, time derivatives, time delays, and running averages. Inputs to the empirical models included time series, or signals, of inflows and outflows from the control structures, precipitation, and evapotranspiration. For a complex hydrologic system like the Refuge, the statistical accuracy of the models and predictive capability were good. The water-level models have coefficient of determination (R 2 values ranging from 0.90 to 0.98. The R 2 for the specific conductance model is 0.82, and the R2 for the total phosphorus model is 0.51. The accuracy of the models was attributable to the quantity and quality of the available data. To make the models directly available to all stakeholders, an easy-to-use decision support system (DSS) called the Loxahatchee Artificial Neural Network Model (LOXANN) DSS was developed as a spreadsheet application that integrates the historical database, linear regression and ANN models, model controls, streaming graphics, and model output. The LOXANN DSS allows Refuge managers and other users to easily execute the water level, specific conductance, and phosphorous models to evaluate various water-resource management scenarios. The user is able to choose from three options in setting the control-structure flows: as a percentage of historical flow, as a constant flow, or as a user-defined hydrograph. Output from the LOXANN DSS includes tabular time series of predictions of the measured data and predictions of the user-specified conditions. A three-dimensional visualization routine also was developed that displays longitudinal specific conductance conditions. Two scenarios were simulated with the LOXANN DSS. One scenario increased the historical flows at four control structures by 40 percent. The second scenario used a user-defined hydrograph to set the outflow from the Refuge to the weekly average inflow to the Refuge delayed by 2 days. Both scenarios decreased the potential of canal water intruding into the marsh by decreasing the slope of the water level between the canals and the marsh.

Scientific Investigations Report

Detailed description of oil shale organic and mineralogical heterogeneity via fourier transform infrared mircoscopy

Mineralogical and geochemical information on reservoir and source rocks is necessary to assess and produce from petroleum systems. The standard methods in the petroleum industry for obtaining these properties are bulk measurements on homogenized, generally crushed, and pulverized rock samples and can take from hours to days to perform. New methods using Fourier transform infrared (FTIR) spectroscopy have been developed to more rapidly obtain information on mineralogy and geochemistry. However, these methods are also typically performed on bulk, homogenized samples. We present a new approach to rock sample characterization incorporating multivariate analysis and FTIR microscopy to provide non-destructive, spatially resolved mineralogy and geochemistry on whole rock samples. We are able to predict bulk mineralogy and organic carbon content within the same margin of error as standard characterization techniques, including X-ray diffraction (XRD) and total organic carbon (TOC) analysis. Validation of the method was performed using two oil shale samples from the Green River Formation in the Piceance Basin with differing sedimentary structures. One sample represents laminated Green River oil shales, and the other is representative of oil shale breccia. The FTIR microscopy results on the oil shales agree with XRD and LECO TOC data from the homogenized samples but also give additional detail regarding sample heterogeneity by providing information on the distribution of mineral phases and organic content. While measurements for this study were performed on oil shales, the method could also be applied to other geological samples, such as other mudrocks, complex carbonates, and soils.

Energy & Fuels

Methods and applications in surface depression analysis

Gridded surface data sets are often incorporated into digital data bases, but extracting information from the data sets requires specialized raster processing techniques different from those historically used on remotely sensed and thematic data. Frequently, the information desired of a gridded surface is directly related to the topologic peaks and pits of the surface. A method for isolating these peaks and pits has been developed, and two examples of its application are presented. The perimeter of a pit feature is the highest-valued closed contour surrounding a minimum level. The method devised for finding all such contours is designed to operate on large raster surfaces. If the data are first inversely mapped, this algorithm will find surface peaks rather than pits. In one example the depressions, or pits, expressed in Digital Elevation Model data, are hydrologically significant potholes. Measurement of their storage capacity is the objective. The potholes are found and labelled as polygons; their watershed boundaries are found and attributes are computed. In the other example, geochemical surfaces, which were interpolated from chemical analyses of irregularly distributed stream sediment samples, were analyzed to determine the magnitude, morphology, and areal extent of peaks (geochemical anomalies).

Conference Paper

Using remote sensing and GIS techniques to estimate discharge and recharge. fluxes for the Death Valley regional groundwater flow system, USA

The recharge and discharge components of the Death Valley regional groundwater flow system were defined by remote sensing and GIS techniques that integrated disparate data types to develop a spatially complex representation of near-surface hydrological processes. Image classification methods were applied to multispectral satellite data to produce a vegetation map. This map provided a basis for subsequent evapotranspiration and infiltration estimations. The vegetation map was combined with ancillary data in a GIS to delineate different types of wetlands, phreatophytes and wet playa areas. Existing evapotranspiration-rate estimates were then used to calculate discharge volumes for these areas. A previously used empirical method of groundwater recharge estimation was modified by GIS methods to incorporate data describing soil-moisture conditions, and a recharge potential map was produced. These discharge and recharge maps were readily converted to data arrays for numerical modelling codes. Inverse parameter estimation techniques also used these data to evaluate the reliability and sensitivity of estimated values.

IAHS-AISH Publication

A technique for estimating heights reached by the 100-year flood on unregulated, nontidal streams in North Carolina

A method for estimating the heights reached by floods having a recurrence interval of 100 years is defined for nontidal streams with unregulated flows in North Carolina. The flood heights are the vertical distance between stream stage at median discharge (50 percent duration) and the 100-year flood stage and are defined for streams draining areas between 1 and 10,000 square miles for each of the three physiographic areas in the State. An illustrated example of how the method can be used in conjunction with topographic maps to estimate flood heights and delineate inundated areas by interpolation is given.

North Carolina

Diagnostic methodology is critical for accurately determining the prevalence of ichthyophonus infections in wild fish populations

Several different techniques have been employed to detect and identify Ichthyophonus spp. in infected fish hosts; these include macroscopic observation, microscopic examination of tissue squashes, histological evaluation, in vitro culture, and molecular techniques. Examination of the peer-reviewed literature revealed that when more than 1 diagnostic method is used, they often result in significantly different results; for example, when in vitro culture was used to identify infected trout in an experimentally exposed population, 98.7% of infected trout were detected, but when standard histology was used to confirm known infected tissues from wild salmon, it detected ~50% of low-intensity infections and ~85% of high-intensity infections. Other studies on different species reported similar differences. When we examined a possible mechanism to explain the disparity between different diagnostic techniques, we observed non-random distribution of the parasite in 3-dimensionally visualized tissue sections from infected hosts, thus providing a possible explanation for the different sensitivities of commonly used diagnostic techniques. Based on experimental evidence and a review of the peer-reviewed literature, we have concluded that in vitro culture is currently the most accurate diagnostic technique for determining infection prevalence of Ichthyophonus, particularly when the exposure history of the population is not known.

Journal of Parasitology

Estimation of base flow on ungaged, periodically measured streams in small watersheds in western Pennsylvania

A 2.5-year data collection program was undertaken by the U.S. Geological Survey, in cooperation with the Pennsylvania Department of Environmental Protection (PADEP), to quantify and estimate base flow in small watersheds in western Pennsylvania where only periodic streamflow measurements had been obtained. Twelve streamgages with watershed areas of less than 10 square miles were established in western Pennsylvania for this study, with most established within Greene and Washington Counties (an area where a type of underground coal mining known as longwall mining occurs). Data from five previously established streamgages with watershed areas ranging from 48.9 to 281 square miles were also used in the analyses for this study. The index-gage method was used to relate streamflow at one streamgage referred to as the “index streamgage” to streamflow at another site of interest (usually an ungaged site, but for this study another streamgage) using a regression technique. Streamflow regressions were developed for all newly established streamgages by using the Maintenance of Variance Extension, Type 1 (MOVE.1) method. Not all streamflow data from the newly established streamgages were used for MOVE.1 regression development; only data that have little to no influence from runoff were considered. Runoff-influenced streamflow for this study was defined as streamflow on a day that precipitation occurs plus streamflow on the following 2 days. One streamflow value per day selected from a specified schedule that captures numerous non-runoff periods was used to develop a MOVE.1 regression. Prediction limits were calculated from the regression to provide the upper and lower bounds for the regression-produced streamflow estimates. Using these data, base flow at a site can be estimated with the index-gage method. The log 10 -tranformed prediction interval width and other regression diagnostics were used as indicators of regression quality when comparing streamgage relations to determine the best index streamgage among the streamgages established for this study. It was determined that index streamgages within about 10 miles of the site of interest provided the best estimated base flow and could, in the future, be used by mine operators and the PADEP to quantify base flow and to evaluate the effects of mining on streamflow.

Pennsylvania

Developing a photography-based harvest survey to estimate age and subspecies composition of midcontinent sandhill cranes

Midcontinent sandhill cranes ( Antigone canadensis ) are managed as a single population, but hunting regulations are structured so harvest is targeted towards the more numerous lesser sandhill cranes ( A. c. canadensis ). However, research indicates that greater sandhill cranes ( A. c. tabida ) have been disproportionally exposed to harvest at a rate exceeding their proportion within the midcontinent population. In addition, harvest has increased 22% per year in the U.S. Central Flyway states. The midcontinent population appears to be growing in recent years, but variability in annual abundance estimates has increased substantially. With limited resources and harvest management uncertainty increasing, we developed methods for a citizen science, photography-based harvest survey to estimate age and subspecies composition of harvested midcontinent sandhill cranes. To develop survey methods, we collected physical parts from 284 sandhill cranes in North Dakota in 2019 and 2020. We manually measured the culmen and tarsus using calipers, and digitally measured these parts using photographs and computer software. All digitally derived measurements were 2.5% to 5.9% larger than manual measurements; therefore, we developed linear models that adjusted digital measurements, facilitating subspecies prediction using an existing morphometric-based technique. In 2021, we requested an equal number of hunters to participate using 2 data collection methods to test if hunters could reliably take photographs suitable for digital measurement. Collection method 1 involved photographing the head and leg simultaneously, and Collection method 2 involved photographing the head only. Hunters submitted a total of 239 photographs. Only 80 of these photographs were submitted using Collection method 1, and 72% were suitable for digital measurement. Conversely, hunters submitted twice as many photographs using Collection method 2, and 88% of these photographs were deemed suitable. Although obtaining the tarsus measurement slightly improved subspecies predictability, Collection method 2 increased participation and usable data. We believe our results could be used to develop an operational survey of age and subspecies composition of midcontinent sandhill cranes, wherein a sample of crane hunters throughout the midcontinent population range would be asked to electronically submit a photograph of the head of each bird they harvest. A time series of age and subspecies composition of this population would provide managers with valuable information and improve harvest management at minimal additional cost and burden, compared to a traditional parts collection survey administered by mail.

Wildlife Society Bulletin

Landsat 7 scan line corrector-off gap-filled product development

The Landsat 7 Enhanced Thematic Mapper Plus (ETM+) scan line corrector (SLC) failed on May 31, 2003, causing the scanning pattern to exhibit wedge-shaped scan-to-scan gaps. The ETM+ has continued to acquire data with the SLC powered off, leading to images that are missing approximately 22 percent of the normal scene area. To improve the utility of the SLC-off data, the U.S. Geological Survey (USGS) developed new products that use the data from multiple ETM+ scenes to provide complete ground coverage. These gap-filled products were developed and deployed in two phases. The gaps in the Phase I products are filled with data from imagery collected previously with a functional SLC (SLC-on). A single SLC-on scene provides complete coverage of the scan gaps, making the gap-filling procedure straightforward. Several radiometric adjustment techniques for matching the SLC-on fill scene to the SLC-off primary scene were evaluated for performance, processing speed, and ease of implementation. A simple local histogram matching method was adopted as a result of this evaluation. The Phase II products use data from multiple SLC-off scenes to fill the scan gaps with more recent data. Because the locations of the scan gaps are different for each SLC-off scene, the gap-filling process must account for scan gap interactions. The Phase II product development included a more comprehensive study of candidate radiometric adjustment techniques. This study showed that the histogram matching method used in Phase I, with minor refinements, provided the best overall performance and was adopted for Phase II as well.

Conference Paper