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Modeling risk of pneumonia epizootics in bighorn sheep

Pneumonia epizootics are a major challenge for management of bighorn sheep ( Ovis canadensis ) affecting persistence of herds, satisfaction of stakeholders, and allocations of resources by management agencies. Risk factors associated with the disease are poorly understood, making pneumonia epizootics hard to predict; such epizootics are thus managed reactively rather than proactively. We developed a model for herds in Montana that identifies risk factors and addresses biological questions about risk. Using Bayesian logistic regression with repeated measures, we found that private land, weed control using domestic sheep or goats, pneumonia history, and herd density were positively associated with risk of pneumonia epizootics in 43 herds that experienced 22 epizootics out of 637 herd-years from 1979–2013. We defined an area of high risk for pathogen exposure as the area of each herd distribution plus a 14.5-km buffer from that boundary. Within this area, the odds of a pneumonia epizootic increased by >1.5 times per additional unit of private land (unit is the standardized % of private land where global  = 25.58% and SD = 14.53%). Odds were >3.3 times greater if domestic sheep or goats were used for weed control in a herd's area of high risk. If a herd or its neighbors within the area of high risk had a history of a pneumonia epizootic, odds of a subsequent pneumonia epizootic were >10 times greater. Risk greatly increased when herds were at high density, with nearly 15 times greater odds of a pneumonia epizootic compared to when herds were at low density. Odds of a pneumonia epizootic also appeared to decrease following increased spring precipitation (odds = 0.41 per unit increase, global  = 100.18% and SD = 26.97%). Risk was not associated with number of federal sheep and goat allotments, proximity to nearest herds of bighorn sheep, ratio of rams to ewes, percentage of average winter precipitation, or whether herds were of native versus mixed or reintroduced origin. We conclude that factors associated with risk of pneumonia epizootics are complex and may not always be from the most obvious sources. The ability to identify high-risk herds will help biologists and managers determine where to focus management efforts and the risk factors that most affect each herd, facilitating more effective, proactive management.

Journal of Wildlife Management↗

Proceedings of a workshop on fish habitat suitability index models

One of the habitat-based methodologies for impact assessment currently in use by the U.S. Fish and Wildlife Service is the Habitat Evaluation Procedures (HEP) (U.S. Fish and Wildlife Service 1980). HEP is based on the assumption that the quality of an area as wildlife habitat at a specified target year can be described by a single number, called a Habitat Suitability Index (HSI). An HSI of 1.0 represents optimum habitat: an HSI of 0.0 represents unsuitable habitat. The verbal or mathematical rules by which an HSI is assigned to an area are called an HSI model. A series of Habitat Suitability Index (HSI) models, described by Schamberger et al. (1982), have been published to assist users in applying HEP. HSI model building approaches are described in U.S. Fish and Wildlife Service (1981). One type of HSI model described in detail requires the development of Suitability Index (SI) graphs for habitat variables believed to be important for the growth, survival, standing crop, or other measure of well-being for a species. Suitability indices range from 0 to 1.0, with 1.0 representing optimum conditions for the variable. When HSI models based on suitability indices are used, habitat variable values are measured, or estimated, and converted to SI's through the use of a Suitability Index graph for each variable. Individual SI's are aggregated into an HSI. Standard methods for testing this type of HSI model did not exist at the time the studies reported in this document were performed. A workshop was held in Fort Collins, Colorado, February 14-15, 1983, that brought together biologists experienced in the use, development, and testing of aquatic HSI models, in an effort to address the following objectives: (1) review the needs of HSI model users; (2) discuss and document the results of aquatic HSI model tests; and (3) provide recommendations for the future development, testing, modification, and use of HSI models. Individual presentations, group discussions, and group decision techniques were used to develop and present information at the meeting. A synthesis of the resulting concepts, results, and recommendations follows this preface. Subsequent papers describe individual tests of selected HSI models. Most of the tests involved comparison of values from HSI models or Suitability index (SI) curves with standing crop, as required contractually. Time and budget constraints generally limited tests to the use of data previously collected for other purposes. These proceedings are intended to help persons responsible for the development, testing, or use of HSI models by increasing their understanding of potential uses and limitations of testing procedures and models based on aggregated Suitability Indices. Problems encountered when testing HSI models are described, model performance during tests is documents, and recommendations for future model development and testing presented by the participants are listed and interpreted.

Biological Report↗

Design and implementation of estimation-based monitoring programs for flora and fauna: A case study on the Cherokee National Forest

Science-based monitoring of biological resources is important for a greater understanding of ecological systems and for assessment of the target population using theoretic-based management approaches. When selecting variables to monitor, managers first need to carefully consider their objectives, the geographic and temporal scale at which they will operate, and the effort needed to implement the program. Generally, monitoring can be divided into two categories: index and inferential. Although index monitoring is usually easier to implement, analysis of index data requires strong assumptions about consistency in detection rates over time and space, and parameters are often biasednot accounting for detectability and spatial variation. In most cases, individuals are not always available for detection during sampling periods, and the entire area of interest cannot be sampled. Conversely, inferential monitoring is more rigorous because it is based on nearly unbiased estimators of spatial distribution. Thus, we recommend that detectability and spatial variation be considered for all monitoring programs that intend to make inferences about the target population or the area of interest. Application of these techniques is especially important for the monitoring of Threatened and Endangered (T&E) species because it is critical to determine if population size is increasing or decreasing with some level of certainty. Use of estimation-based methods and probability sampling will reduce many of the biases inherently associated with index data and provide meaningful information with respect to changes that occur in target populations. We incorporated inferential monitoring into protocols for T&E species spanning a wide range of taxa on the Cherokee National Forest in the Southern Appalachian Mountains. We review the various approaches employed for different taxa and discuss design issues, sampling strategies, data analysis, and the details of estimating detectability using site occupancy. These techniques provide a science-based approach for monitoring and can be of value to all resource managers responsible for management of T&E species.

Proceedings of the Annual Conference of the Southe↗

Evaluation of nutria (Myocastor coypus) detection methods in Maryland, USA

Nutria ( Myocaster coypus ), invasive, semi-aquatic rodents native to South America, were introduced into Maryland near Blackwater National Wildlife Refuge (BNWR) in 1943. Irruptive population growth, expansion, and destructive feeding habits resulted in the destruction of thousands of acres of emergent marshes at and surrounding BNWR. In 2002, a partnership of federal, state and private entities initiated an eradication campaign to protect remaining wetlands from further damage and facilitate the restoration of coastal wetlands throughout the Chesapeake Bay region. Program staff removed nearly 14,000 nutria from five infested watersheds in a systematic trapping and hunting program between 2002 and 2014. As part of ongoing surveillance activities, the Chesapeake Bay Nutria Eradication Project uses a variety of tools to detect and remove nutria. Project staff developed a floating raft, or monitoring platform, to determine site occupancy. These platforms are placed along waterways and checked periodically for evidence of nutria visitation. We evaluated the effectiveness of monitoring platforms and three associated detection methods: hair snares, presence of scat, and trail cameras. Our objectives were to (1) determine if platform placement on land or water influenced nutria visitation rates, (2) determine if the presence of hair snares influenced visitation rates, and (3) determine method-specific detection probabilities. Our analyses indicated that platforms placed on land were 1.5–3.0 times more likely to be visited than those placed in water and that platforms without snares were an estimated 1.7–3.7 times more likely to be visited than those with snares. Although the presence of snares appears to have discouraged visitation, seasonal variation may confound interpretation of these results. Scat was the least effective method of determining nutria visitation, while hair snares were as effective as cameras. Estimated detection probabilities provided by occupancy modeling were 0.73 for hair snares, 0.71 for cameras and 0.40 for scat. We recommend the use of hair snares on monitoring platforms as they are the most cost-effective and reliable detection method available at this time. Future research should focus on determining the cause for the observed decrease in nutria visits after snares were applied.

Maryland↗

Seasonal resource selection and movement ecology of free-ranging horses in the western United States

Understanding factors driving resource selection and habitat use of different species is an important component of management and conservation. Feral horses ( Equus caballus ) are free ranging across various vegetation types in the western United States, yet few studies have quantified their resource selection and seasonal use. We conducted a study to determine effects of vegetation community, distance to water, and topographic variables on seasonal resource selection in 2 feral horse populations in Great Basin sagebrush ( Artemisia spp.) ecosystems of west-central Utah, USA: Conger Herd Management Area (HMA) and Frisco HMA. We deployed global positioning system (GPS) radio-collars on 38 female horses and GPS-transmitters braided and glued into the tail hair of 14 males, collecting locations every 2 hours for 1–4 years between 2016 and 2020. We calculated home range size and core use area of social groups (harems) and bachelor males using auto-correlated kernel density estimators for each biologically defined season (breeding, fall, and winter) per study year. We examined seasonal home range size and overlap of harem groups and bachelor males and compared movement speed of bachelors and harems among seasons. We determined seasonal resource selection in a use-availability framework using resource selection functions. We hypothesized that horses would select for areas of high herbaceous vegetation, that water would be a key variable in resource selection models like other equids, and home range size in winter would be largest because horses can eat snow for hydration and could therefore roam farther from surface water. Mean annual home range size was 103.12 ± 37.38 km 2 (SD) for Conger harems and 117.47 ± 32.75 km 2 for Frisco harems. At Conger there was no difference in home range size between harem groups and bachelor males, but home range size was smaller in winter than other seasons, whereas winter home range size at Frisco was larger than other seasons. Bachelor males moved at higher speeds than harems during all seasons, and harem groups from both populations had lower movement speeds in winter. Harem groups had distinct winter ranges with little overlap on breeding season ranges. In both populations, all horses selected for herbaceous vegetation types and avoided forest relative to shrubland throughout the year. Harems at Frisco were consistently located closer to water sources, whereas selection for water sources by Conger harems varied seasonally, with winter having the lowest selection. Harem groups at Conger had an average of 10.6% of their home ranges outside the HMA boundary and Frisco harems had up to 66.8% outside, likely because of the horseshoe shape of Frisco HMA in which shrub meadows (foraging areas) comprise the horseshoe center, which is outside the HMA. Our results highlight the importance of water sources, which were a key predictor of horse movement patterns in our study. We emphasize the utility of telemetry devices to understand resource selection of feral horses at a fine scale, enabling management to be more targeted and facilitate planning.

Utah↗

Spatial-temporal patterns in Mediterranean carnivore road casualties: Consequences for mitigation

Many carnivores have been seriously impacted by the expansion of transportation systems and networks; however we know little about carnivore response to the extent and magnitude of road mortality, or which age classes may be disproportionately impacted. Recent research has demonstrated that wildlife-vehicle-collisions (WVC) involving carnivores are modulated by temporal and spatial factors. Thus, we investigated road mortality on a guild of small and medium-sized carnivores in southern Portugal using road-kill data obtained from a systematic 36 months monitoring period along highways (260 km) and national roads (314 km) by addressing the following questions: (a) which species and age class are most vulnerable to WVC? (b) are there temporal and/or spatial patterns in road-kill? and (c) which life-history and/or spatial factors influence the likelihood of collisions? We recorded a total of 806 carnivore casualties, which represented an average of 47 ind./100 km/year. Red fox and stone marten had the highest mortality rates. Our findings highlight three key messages: (1) the majority of road-killed individuals were adults of common species; (2) all carnivores, except genets, were more vulnerable during specific life-history phenological periods: higher casualties were observed when red fox and stone marten were provisioning young, Eurasian badger casualties occurred more frequently during dispersal, and higher Egyptian mongoose mortality occurred during the breeding period; and (3) modeling demonstrated that favorable habitat, curves in the road, and low human disturbance were major contributors to the deadliest road segments. Red fox carcasses were more likely to be found on road sections with passages distant from urban areas. Conversely, stone marten mortalities were found more often on national roads with high of cork oak woodland cover; Egyptian mongoose and genet road-kills were found more often on road segments close to curves. Based on our results, two key mitigation measures should help to reduce WVC in Portugal. The first involves the improvement of existing crossings with buried and small mesh size fence to guide the individuals towards to the passages, in road segments with high traffic volume (>1200 vehicles/night) and located in preferred carnivore habitats. The second mitigation involves cutting or removal of dense vegetation in verges of road segments with curves to aid motorists in seeing animals about to cross. ?? 2008 Elsevier Ltd.

Biological Conservation↗

Gear comparison study for sampling nekton in Barataria Basin marshes

This project was funded by the Louisiana Trustee Implementation Group (LA TIG) to support decisions related to investments in long-term monitoring. The LA TIG seeks to ensure long-term monitoring informs coastal restoration activities with the goal of sustaining and improving fisheries impacted by the Deepwater Horizon (DWH) Oil Spill. The project objective was to compare nekton catch across an estuarine gradient using different sampling gear with the goal of identifying trade-offs among nekton sampling approaches. To accomplish this objective, Louisiana Department of Wildlife and Fisheries (LDWF), The Water Institute of the Gulf (the Institute), Dynamic Solutions, LLC, Louisiana State University Agricultural Center (LSU AgCenter), and the U.S. Geological Survey (USGS) completed a field gear comparison study from 2018 to 2019. This work compared electrofisher and seine sampling at 12 fixed stations in Barataria Basin using data collected by LDWF. In addition, and in conjunction with LDWF monthly sampling, the same 12 fixed stations were sampled in May 2019 using a throw trap to compare nekton catch and assemblages collected with the throw trap, seine and electrofisher. LDWF has been conducting seine sampling since 1986, and seine data are used by the State of Louisiana to assess juvenile shrimp, crab and fish abundances, sizes and overall assemblages. In 2018, LDWF began conducting electrofisher sampling at 12 Barataria Basin seine stations in order to determine if the two gear types sample similar species and assemblages for potential future replacement of long-term seine sampling with electrofishing. Throw traps were included as they provide density estimates, which are ultimately the desired statistic used in modeling trophic webs, and are used in assessing habitat restoration outcomes. The project compared the nekton catch and assemblages collected using seine, electrofisher, and throw trap data from marsh edge habitats located across the estuarine gradient in Barataria Basin. Specifically, catch per unit effort (CPUE), species richness, species-specific total length (mm) distribution and nekton assemblages were compared between gear types. The first dataset was collected in May 2019 with throw trap (Appendix A), seine (LDWF data), and electrofisher (LDWF data) gear, and the second dataset (collected by LDWF) spanned 14 months of seine and electrofisher monthly sampling occurring from May 2018 through June 2019 at 12 stations in Barataria Basin. Key findings include that gear bias was not evident across the range of water quality conditions (salinity, temperature, o C, dissolved oxygen, mg L-1 , turbidity, NTU; Appendix B: scatter plots) captured during this pilot study, but differences in nekton catch per unit effort (CPUE) and assemblages were evident between gear types. However, those differences largely depended on the parameter examined. For example, the overall CPUE was highest for electrofishing, followed by seine, and then throw trap. When grass shrimp (the most abundant taxon collected) were removed from CPUE, the electrofisher and seine results were similar in CPUE. When CPUE was corrected for gear efficiency and total area sampled, the throw trap had the highest reported density of nekton sampled, followed by electrofisher and seine results. Electrofishing captured the highest number of species, which included more unique species compared to seine or throw trap catches, though all gear types captured at least one unique species. These highlight a need for caution in interpreting assemblage and density data when comparing datasets derived from different sampling methodologies. These key findings can help inform implementation and interpretation of long-term monitoring data in Louisiana as management decisions are made about coastal restoration projects to sustain and improve fisheries. There are trade-offs in selecting gear types for estuarine nekton monitoring of density, abundance, species richness, and assemblages. The table below (Table 1) summarizes some considerations when selecting gear types for long-term monitoring of estuarine nekton. In addition to biological and ecological considerations, other important considerations include cost, the labor required to conduct sampling, logistics, and potential uncertainties related to how effective each gear type is for sampling the wide variety of conditions found across Louisiana’s coastal habitats. For example, although electrofishing may capture higher CPUE, the equipment is more expensive to obtain and maintain compared to the other gear types. Most importantly, this table highlights differences in the nekton assemblages sampled by each gear type; this consideration is critical when designing the goals of a long-term monitoring program as it will inform how the data can be used and interpreted in the future. This report provides caveats, assumptions, and recommendations that can help support the Louisiana Coastal Protection and Restoration Authority (CPRA), LDWF and the LA TIG in comparing data from different gear types, and in making decisions for future monitoring. Findings from this study are limited to the range of water quality conditions occurring during these data collection events; these data and analyses could benefit from sampling across a wider range of water quality conditions, and collection of habitat structure and bottom type data which are not routinely collected but critically influence nekton. Further investigation examining how relative differences detected in key species abundances between gear types might impact ecosystem indicators and energetics in a modeled food web would provide valuable input to understand outputs of the Comprehensive Aquatic System Model for Barataria Basin, including the potential impacts of nekton monitoring decisions on food web models.

Louisiana↗

Habitat prioritization across large landscapes, multiple seasons, and novel areas: an example using greater sage-grouse in Wyoming

Animal habitat selection is an important and expansive area of research in ecology. In particular, the study of habitat selection is critical in habitat prioritization efforts for species of conservation concern. Landscape planning for species is happening at ever-increasing extents because of the appreciation for the role of landscape-scale patterns in species persistence coupled to improved datasets for species and habitats, and the expanding and intensifying footprint of human land uses on the landscape. We present a large-scale collaborative effort to develop habitat selection models across large landscapes and multiple seasons for prioritizing habitat for a species of conservation concern. Greater sage-grouse ( Centrocercus urophasianus , hereafter sage-grouse) occur in western semi-arid landscapes in North America. Range-wide population declines of this species have been documented, and it is currently considered as “warranted but precluded” from listing under the United States Endangered Species Act. Wyoming is predicted to remain a stronghold for sage-grouse populations and contains approximately 37% of remaining birds. We compiled location data from 14 unique radiotelemetry studies (data collected 1994–2010) and habitat data from high-quality, biologically relevant, geographic information system (GIS) layers across Wyoming. We developed habitat selection models for greater sage-grouse across Wyoming for 3 distinct life stages: 1) nesting, 2) summer, and 3) winter. We developed patch and landscape models across 4 extents, producing statewide and regional (southwest, central, northeast) models for Wyoming. Habitat selection varied among regions and seasons, yet preferred habitat attributes generally matched the extensive literature on sage-grouse seasonal habitat requirements. Across seasons and regions, birds preferred areas with greater percentage sagebrush cover and avoided paved roads, agriculture, and forested areas. Birds consistently preferred areas with higher precipitation in the summer and avoided rugged terrain in the winter. Selection for sagebrush cover varied regionally with stronger selection in the Northeast region, likely because of limited availability, whereas avoidance of paved roads was fairly consistent across regions. We chose resource selection function (RSF) thresholds for each model set (seasonal × regional combination) that delineated important seasonal habitats for sage-grouse. Each model set showed good validation and discriminatory capabilities within study-site boundaries. We applied the nesting-season models to a novel area not included in model development. The percentage of independent nest locations that fell directly within identified important habitat was not overly impressive in the novel area (49%); however, including a 500-m buffer around important habitat captured 98% of independent nest locations within the novel area. We also used leks and associated peak male counts as a proxy for nesting habitat outside of the study sites used to develop the models. A 1.5-km buffer around the important nesting habitat boundaries included 77% of males counted at leks in Wyoming outside of the study sites. Data were not available to quantitatively test the performance of the summer and winter models outside our study sites. The collection of models presented here represents large-scale resource-management planning tools that are a significant advancement to previous tools in terms of spatial and temporal resolution.

Wyoming↗

Quantitative meta-analysis reveals no association between mercury contamination and body condition in birds

Mercury contamination is a major threat to the global environment, and is still increasing in some regions despite international regulations. The methylated form of mercury is hazardous to biota, yet its sublethal effects are difficult to detect in wildlife. Body condition can vary in response to stressors, but previous studies have shown mixed effects of mercury on body condition in wildlife. Using birds as study organisms, we provide the first quantitative synthesis of the effect of mercury on body condition in animals. In addition, we explored the influence of intrinsic, extrinsic and methodological factors potentially explaining cross-study heterogeneity in results. We considered experimental and correlative studies carried out in adult birds and chicks, and mercury exposure inferred from blood and feathers. Most experimental investigations (90%) showed a significant relationship between mercury concentrations and body condition. Experimental exposure to mercury disrupted nutrient (fat) metabolism, metabolic rates, and food intake, resulting in either positive or negative associations with body condition. Correlative studies also showed either positive or negative associations, of which only 14% were statistically significant. Therefore, the overall effect of mercury concentrations on body condition was null in both experimental (estimate ± SE = 0.262 ± 0.309, 20 effect sizes, five species) and correlative studies (−0.011 ± 0.020, 315 effect sizes, 145 species). The single and interactive effects of age class and tissue type were accounted for in meta-analytic models of the correlative data set, since chicks and adults, as well as blood and feathers, are known to behave differently in terms of mercury accumulation and health effects. Of the 15 moderators tested, only wintering status explained cross-study heterogeneity in the correlative data set: free-ranging wintering birds were more likely to show a negative association between mercury and body condition. However, wintering effect sizes were limited to passerines, further studies should thus confirm this trend in other taxa. Collectively, our results suggest that ( i ) effects of mercury on body condition are weak and mostly detectable under controlled conditions, and ( ii ) body condition indices are unreliable indicators of mercury sublethal effects in the wild. Food availability, feeding rates and other sources of variation that are challenging to quantify likely confound the association between mercury and body condition in natura . Future studies could explore the metabolic effects of mercury further using designs that allow for the estimation and/or manipulation of food intake in both wild and captive birds, especially in under-represented life-history stages such as migration and overwintering.

Biological Reviews↗

Pneumonia in bighorn sheep: Risk and resilience

Infectious disease was an important driver of historic declines and extirpations of bighorn sheep (Ovis canadensis) in North America and continues to impede population restoration and management. Domestic sheep have long been linked to pneumonia outbreaks in bighorn sheep and this association has now been confirmed in 13 captive commingling experiments. However, ecological and etiological complexities still hinder our understanding and control of the disease. We provide an overview of the current state of knowledge about the biology and management of respiratory disease in bighorn sheep and propose strategies for moving forward. Epizootic pneumonia in bighorn sheep is polymicrobial. Mycoplasma ovipneumoniae, a bacterium host-specific to Caprinae and commonly carried by healthy domestic sheep and goats appears to be a necessary primary agent. All-age epizootics following introduction of M. ovipneumoniae along with other pathogens into bighorn sheep populations are usually severe (median mortality 47%) but fatality rates vary widely, from 15 – 100%. Disease severity may be influenced by the strain of M. ovipneumoniae, by secondary bacterial and viral pathogens, and by factors affecting transmission and host immunity. Once introduced, M. ovipneumoniae can persist in bighorn sheep populations for decades. Carrier dams transmit the pathogen to their susceptible lambs, triggering fatal pneumonia outbreaks in nursery groups, which limits recruitment and slows or prevents population recovery. The result is that demographic costs of pathogen persistence often outweigh the impacts of the initial invasion and die-off. There is currently no effective vaccine or antibiotic for domestic or wild sheep and to date, no management actions have been successful in reducing morbidity, mortality, or disease spread once pathogen invasion has occurred. Molecular-based strain typing suggests that spillover of M. ovipneumoniae into bighorn sheep populations from domestic small ruminants is ongoing, and that consequences of pathogen invasion are amplified by movements of infected bighorn sheep. Therefore, current disease management strategies focus on reducing risk of spillover from reservoir populations of domestic small ruminants and on limiting transmission among bighorn sheep. A broad array of approaches has been tried and more are needed to prevent pathogen introduction, induce disease fadeout in persistently infected populations, and promote population resilience across the diverse landscapes bighorn sheep inhabit. A comprehensive examination of disease dynamics across populations could help elucidate how disease fades out naturally and if population resilience can be increased in the face of infection. Cross-jurisdictional adaptive management experiments and transdisciplinary collaboration, including partnerships with members of the domestic sheep and goat community, are needed to facilitate innovation and speed progress towards sustainable solutions for managing pneumonia to protect and restore bighorn sheep populations.

California, Idaho, Nevada, Oregon, Utah, Washingto↗

Habitat selection, movement patterns, and hazards encountered by northern leopard frogs (Lithobates pipiens) in an agricultural landscape

Telemetry data for 59 Northern Leopard Frogs (Lithobates pipiens) breeding in ponds in Houston and Winona Counties, MN; 2001-2002. Agricultural intensification is causing declines in many wildlife species, including Northern Leopard Frogs (Lithobates pipiens). Specific information about frog movements, habitat selection, and sources of mortality can be used to inform conservation-focused land management and acquisition. We studied Northern Leopard Frogs in southeastern Minnesota, part of the Driftless Area ecoregion, characterized by hills and valleys and a mix of agriculture, forests, small towns and farmsteads. In this area, small farm ponds, originally built to control soil erosion are used by the species for breeding and wintering in addition to riparian wetlands. But, this agricultural landscape may be hazardous for frogs moving between breeding, feeding, and wintering habitats. We surgically implanted transmitters into the peritoneal cavity of 59 Northern Leopard Frogs and tracked them from May to October 2001-2002. The total distance traveled by radio-tagged frogs ranged from 12 to 3316 m, the 95% home range averaged 5.3 ± 1.2 (SE) ha, and the 50% core area averaged 1.05 ± 0.3 (SE) ha. As expected, Northern Leopard Frogs selected wetlands over all other land cover classes and row crops were generally avoided at all levels of selection. Only a few tracked frogs were successful at dispersing (n = 6). Most frogs attempting to disperse (n =31) ended up missing (n = 14), died due to mowing (n = 8), or were recorded as transmitter failure (n = 2) or unknown mortalities (n = 1). For the conservation of Northern Leopard Frogs in this agricultural setting, we must consider both the aquatic and the terrestrial needs of this species. Conservation agencies that restore, manage, and acquire wetlands should consider the hazards posed by land uses adjacent to frog breeding and wintering sites and plan for movement corridors between these locations. For example, grasslands that are mowed or hayed between April and October in the north central U.S. and are adjacent to wetlands, pose a direct threat to frogs because these cultivated grasslands are primary locations for summer occupancy. When conservation land managers are selecting sites for acquisition or restoration they should avoid investments that will situate the wetland adjacent to heavily travelled roads and agricultural lands likely to be mowed or hayed. Increasing habitat amount and quality at amphibian breeding, feeding and wintering sites should reduce the energy required and hazards associated with moving long distances. Large, diverse wetlands probably provide all of the requirements needed by Northern Leopard Frogs for survival including food, shelter, breeding and overwintering areas.

Minnesota↗

Procedures and rationale for marking sea otters captured and treated during the T/V Exxon Valdez oil spill

Four methods were used for marking sea otters ( Enhydra lutris ) captured and treated during the response to the T/V Exxon Valdez oil spill. Colored and numbered flipper tags were placed on each sea otter that was brought to the otter treatment centers. These tags allowed individual recognition and permitted the tracking of individuals through the treatment and holding processes. Recovery of tagged carcasses may provide a crude measure of the fates of rehabilitated otters. Seven sea otters were instrumented with radio transmitters attached to flipper tags as part of a pilot release program. The results of that study were inconclusive. Forty-five sea otters were implanted with radio transmitters as part of a study to assess the fate of rehabilitated sea otters. Specific objectives of the study include estimating survived rates and monitoring the reproductive success of the sample of rehabilitated sea otters and comparing the results with similar variables in a control population. Transponder chips were injected in the perianal region of all but seven of the instrumented sea otters, and all were tagged with red flipper tags. The advantages and disadvantages of each type of tag are discussed.

Alaska↗

Waterfowl migration on Klamath Basin National Wildlife Refuges 1953-2001

The Klamath Basin National Wildlife Refuge (NWR) complex, located in northeastern California and southern Oregon, is situated on a major Pacific Flyway migration corridor connecting waterfowl breeding grounds in the north with major wintering grounds in California and Mexico. The complex comprises five waterfowl refuges including Lower Klamath NWR, Tule Lake NWR, Upper Klamath NWR, Klamath Marsh NWR, and Clear Lake NWR, and one bald eagle refuge, Bear Valley NWR. Lower Klamath and Tule Lake NWRs are the largest refuges in the complex; historically, they supported some of the greatest autumn and spring concentrations of migrating waterfowl in North America. Starting in 1953, standardized waterfowl surveys from small aircraft have been conducted in autumn through spring. This report summarizes waterfowl migration activity (i.e., abundance, species composition, distribution on refuges, and chronology) over four time periods—the long-term (1953-2001), early (1953-76), recent (1977-2001), and the most recent (1998-2001)—to describe changing patterns of migration on Klamath Basin refuges from autumn 1953 to spring 2001. Over the long term, waterfowl abundance (birds per day) on the refuge complex averaged about 1.0 million in autumn and about 360,000 in spring. A record peak count of 5.8 million waterfowl was recorded September 24-25, 1958. Average abundance of autumn staging waterfowl for the refuge complex, after reaching record levels in the 1950s and early 1960s, began a decline that lasted until the 1980s. A gradual recovery occurred during the 1990s, but autumn abundance has not recovered to pre-1970 levels. In contrast to autumn, average spring abundance was generally lower in the early decades but has gradually increased through the 1990s, particularly on Lower Klamath NWR. Dabbling ducks represented an average of 68% of all waterfowl in autumn and 55% in spring for the long term. Northern pintail ( Anas acuta ) was dominant, representing 62% of all dabblers in autumn and 51% in spring. A significant decline in pintail abundance starting in the late 1950s altered waterfowl composition on Klamath Basin refuges. As pintail declined, other species such as mallard ( Anas platyrhynchos ) and green-winged teal ( Anas crecca ) increased in abundance. Although Arctic nesting geese, including white-fronted ( Anser albifrons ), cackling Canada ( Branta canadensis minima ), white geese (lesser snow [ Chen caerulescens caerulescens ], and Ross’s [ Chen rossii ]) have become less prominent in recent decades, they reached an historically high abundance during autumn in the 1960s and 1970s, particularly on Tule Lake NWR. Tule Lake NWR supported the highest average autumn waterfowl populations until surpassed by Lower Klamath NWR around 1980. During the recent period (1977-2001), Lower Klamath NWR accounted for 60% of all waterfowl using the refuge complex in autumn and 61% in spring. Habitat diversity and wetland productivity contributed to its greater waterfowl abundance. Tule Lake NWR supported the most geese over the long term, 79% in autumn and 66% in spring; however, total waterfowl abundance on this refuge in autumn has been in decline, likely because of reduced diversity and productivity of sumps in the refuge. Upper Klamath, Klamath Marsh, and Clear Lake NWRs accounted for less than 8% of total waterfowl use in autumn and spring but provided diverse habitats for migrants. Waterfowl use-days on Klamath Basin refuges typically peaked in mid-autumn, decreased as migrants passed through the basin, and then reached a lesser peak during spring passage. Waterfowl abundance reached a pronounced peak in autumn during the early period (1953-76), but spring peak buildup was much less pronounced. For the recent period the autumn peak was more subdued. Waterfowl abundance, species composition, and distribution on Klamath Basin refuges have fluctuated over the decades and have been influenced by events such as productivity on breeding grounds and habitat conditions on wintering grounds that cause shifts in migration patterns. A major challenge for the future appears to be the availability of adequate water for wetland management on Klamath Basin refuges.

California, Oregon↗