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At least 109 records · Page 6Linked to original sources

Advancements towards selective barrier passage by automatic species identification: Applications of deep convolutional neural networks on images of dewatered fish

Invasive species negatively affect enterprises such as fisheries, agriculture, and international trade. In the Laurentian Great Lakes Basin, threats include invasive sea lamprey ( Petromyzon marinus ) and the four major Chinese carps. Barriers have proven to be an effective mechanism for managing invasive species but are detrimental in that they also limit the migration of desirable, native species. Fish passage technologies that selectively pass desirable species while blocking undesirable species are needed. Key to an automated selective barrier passage system is a high precision fish classifier to assign fish to be passed or blocked. Presented is an evaluation of two classifiers developed using images of partially dewatered fish captured from a commercial, high-speed camera array. For a lamprey vs. non-lamprey classification task, an ensemble prediction approach achieved near perfect accuracy on both a validation and test dataset. For a species classification task for 13 species found in the Great Lakes region, an ensemble prediction approach achieved accuracies of 96% and 97% on a validation and test dataset, respectively. Both prediction approaches were based on deep convolutional neural networks constructed using transfer learning and image augmentation. The study provides an important proof-of-concept for the viability in fully automated, selective fish passage systems.

Illinois, Michigan,Ohio↗

Burbot (Lota lota) exhibit plasticity in life-history traits in a small drainage at the southwestern-most extent of the species’ native range

Little is known about the life-history traits exhibited by burbot (Lota lota) throughout their circumpolar range. Monitoring PIT-tagged burbot between lentic and lotic habits and collection of demographic data (length, age, sex, and maturity) were used to answer the following questions in the Torrey Creek drainage of west-central Wyoming, USA: 1) is there plasticity in the life-history traits of the burbot population, 2) do Trail Lake origin and Torrey Creek origin burbot interchange during the spawning period, and 3) is there a difference in growth and age at sexual maturity between Trial Lake and Torrey Creek? We found plasticity in life history traits exists within a burbot population in a small drainage at the southwestern-most edge of the species native range. Directional migration of PIT-tagged burbot in Trail Lake and Torrey Creek was monitored nearly continuously by tandem stream-width antennas. Thirty-five percent of lentic-origin burbot migrated upstream into Torrey Creek, and 11% of lotic-origin burbot migrated downstream of the antennas near the Torrey Creek inlet to Trail Lake. Migratory activity of burbot was highest during the late winter and early spring at a time that coincided with spawning. Additionally, Torrey Creek-origin burbot were smaller and younger than Trail Lake-origin burbot. In addition to documenting migration, the sampling of small, sexually mature burbot in Torrey Creek suggests that stream-resident burbot reach maturity earlier than adfluvial and lacustrine burbot in Trail Lake. Furthermore, high catch rates of age-0 burbot indicate that Torrey Creek upstream from Trail Lake provides nursery habitat to the burbot population. Life history trait plasticity and spawning and nursery habitats documented in this study must be considered when selecting conservation actions for this unique burbot population.

Wyoming↗

Development of sea lamprey ( Petromyzon marinus ) larvicides

Larvicides are used to control sea lamprey ( Petromyzon marinus ) in the Great Lakes. These larvicides are useful because they are more toxic to sea lamprey than fish species found in the same habitat. The lampricides come from two classes of chemical compounds: (1) halonitrophenols, and (2) halonitrosalicylanilides. Selectivity of the larvicides appears to be based on the differences in the ability of sea lamprey larvae and fishes to detoxify and/or excrete the chemicals. Glucuronide conjugation is an important mechanism for detoxification of these larvicides by fish, and selectivity of larvicides may be due to differences in glucuronyl transferase activity between lamprey and fishes. If more detailed information were available on uptake, metabolism, excretion, and the biochemistry and physiology of lamprey as compared to fishes, it might be possible to design chemicals that would be more selective than those now in use.

Canadian Journal of Fisheries and Aquatic Sciences↗

Septicemic pasteurellosis in free-ranging neonatal pronghorn in Oregon

As part of a study to determine the cause(s) of population decline and low survival of pronghorn ( Antilocapra americana ) neonates on Hart Mountain National Antelope Refuge (HMNAR), Oregon (USA), 55 of 104 neonates captured during May 1996 and 1997 were necropsied (n = 28, 1996; n = 27, 1997) to determine cause of death. Necropsies were conducted on fawns that died during May, June, or July of each year. The objectives of this study were to report the occurrence and pathology of pasteurellosis in neonates and determine if the isolated strain of Pasteurella multocida was unique. Septicemic pasteurellosis, caused by P. multocida , was diagnosed as the cause of death for two neonates in May and June 1997. Necropsy findings included widely scattered petechial and ecchymotic hemorrhages found over a large portion of the subcutaneous tissue, meninges of the brain, epicardium, skeletal muscle, and serosal surface of the thorasic and abdominal cavities. Histological examination of lung tissues revealed diffuse congestion and edema and moderate to marked multifocal infiltrate of macrophages, neutrophils, and numerous bacteria within many terminal bronchioles and alveoli. Pasteurella multocida serotypes A:3,4, and B:1 were isolated from several tissues including lung, intestinal, thorasic fluid, and heart blood. Each B:1 isolate had DNA restriction endonuclease fingerprint profiles distinct from isolates previously characterized from domestic cattle, swan ( Olor spp.), moose ( Alces alces ), and pronghorn from Montana (USA). This is the first report of pasteurellosis in pronghorn from Oregon and the B:1 isolates appear to be unique in comparison to DNA fingerprint profiles from selected domestic and wild species.

Oregon↗

Olympic Fisher Reintroduction Project: Progress report 2008-2011

This progress report summarizes the final year of activities of Phase I of the Olympic fisher restoration project. The intent of the Olympic fisher reintroduction project is to reestablish a self-sustaining population of fishers on the Olympic Peninsula. To achieve this goal, the Olympic fisher reintroduction project released 90 fishers within Olympic National Park from 2008 to 2010. The reintroduction of fishers to the Olympic Peninsula was designed as an adaptive management project, including the monitoring of released fishers as a means to (1) evaluate reintroduction success, (2) investigate key biological and ecological traits of fishers, and (3) inform future reintroduction, monitoring, and research efforts. This report summarizes reintroduction activities and preliminary research and monitoring results completed through December 2011. The report is non-interpretational in nature. Although we report the status of movement, survival, and home range components of the research, we have not completed final analyses and interpretation of research results. Much of the data collected during the monitoring and research project will be analyzed and interpreted in the doctoral dissertation being developed by Jeff Lewis; the completion of this dissertation is anticipated prior to April 2013. We anticipate that this work, and analyses of other data collected during the project, will result in several peer-reviewed scientific publications in ecological and conservation journals, which collectively will comprise the final reporting of work summarized here. These publications will include papers addressing post-release movements, survival, resource selection, food habits, and age determination of fishers.

Report↗

Annotated bibliography of scientific research on greater sage-grouse published from 2015 to 2019

The greater sage-grouse ( Centrocercus urophasianus ; hereafter GRSG) has been a focus of scientific investigation and management action for the past two decades. The 2015 U.S. Fish and Wildlife Service listing determination of “not warranted” was in part due to a large-scale collaborative effort to develop strategies to conserve GRSG populations and their habitat and to reduce threats to both. New scientific information augments existing knowledge and can help inform updates or modifications to existing plans for managing GRSG and sagebrush ecosystems. However, the sheer number of scientific publications can be a challenge for managers tasked with evaluating and determining the need for potential updates to existing planning documents. To assist in this process, the U.S. Geological Survey (USGS) has reviewed and summarized the scientific literature published since January 1, 2015. The first GRSG literature summary was published early in 2018. Here we provide an update to that document by adding summaries of articles published between January 6, 2018 and October 2, 2019. To identify articles and reports published about GRSG, we first conducted a structured search of three reference databases (Web of Science, Scopus, and Google Scholar) using the search term “greater sage-grouse.” We refined the initial list of products by (1) removing duplicates, (2) excluding products that were not published as research or scientific review articles in peer-reviewed journals or as formal technical reports, and (3) retaining only those products for which GRSG or their habitat was a research focus. We summarized the contents of each product by using a consistent structure (background, objectives, methods, location, findings, and implications) and assessed the content of each product relevant to a list of 31 management topics. These topics include GRSG biology and habitat characteristics along with potential management actions, land uses, and environmental factors related to GRSG management and conservation. We also noted which articles/reports created new geospatial data. Our original search, conducted on January 7, 2018, and the application of our criteria, resulted in the inclusion of 169 published products (2 of these products were published corrections to journal articles). This update adds summaries of 69 products published between then and October 2, 2019. The management topics most commonly addressed were GRSG behavior or demographics and GRSG habitat selection or habitat characteristics at broad or site scales. Few products addressed captive breeding, recreation, wild horses and burros, and range management structures (including fences). The management topics with the largest increase in representation between the 2018 GRSG literature summary and this update were GRSG survival and GRSG population estimates or targets, which were each addressed in 16 percent of products in the original literature summary document, but were addressed in 30 and 33 percent, respectively, of newly summarized products. Topics with the largest declines in representation were conifer expansion, - 17 to 10 percent, and new geospatial data, -31 to 21 percent. We include in this annotated bibliography the full citation, Digital Object Identifier (DOI), product summary, and management topics addressed by each product. The online version of this bibliography (https://apps.usgs.gov/gsgbib/index.php) is searchable by topic and location and includes links to journal landing pages for each original publication. A substantial body of literature has been compiled on research explicitly related to the conservation, management, monitoring, and assessment of GRSG. These studies may inform planning and management actions that seek to balance conservation, economic, and social objectives and manage diverse resource uses and values across the western United States. The review process for this product included requesting input on each summary from one or more authors of the original peer-reviewed article or report and a formal review of the entire document by three independent reviewers for the original document and by two independent reviewers for the updated document and, subsequently, the USGS Bureau Approving Official. This process is consistent with USGS Fundamental Science Practices.

Arizona, California, Colorado, Idaho, Kansas, Mont↗

Determination of selection gradients using multiple regression versus Structural Equation Modeling (SEM)

Selection studies involving multiple intercorrelated independent variables have employed multiple regression analysis as a means to estimate and partition natural and sexual selection's direct and indirect effects. These statistical models assume that independent variables are measured without error. Most would conclude that such is not the case in the field studies for which these methods are employed. We demonstrate that the distortion of estimates resulting from error variance is not trivial. When independent variables are intercorrelated, extreme distortions may occur. We propose to use Structural Equation Models (SEM), to estimate error variance and produce highly accurate coefficients for formulation of selection gradients. This method is particularly appropriate when the selection is viewed as happening at the level of the latent variables.

Biometrical Journal↗

Analysis and simulation of reactive transport of metal contaminants in ground water in Pinal Creek Basin, Arizona

Large-scale mining activities have generated a plume of acidic ground water more than 15 km long in the regional aquifer of the Pinal Creek Basin. A one-dimensional reactive-transport model was developed using PHREEQC to aid in the analysis of transport and chemical processes in the plume and to determine the uses and limitations of this type of modeling approach. In 1984, the acidic part of the plume had a pH as low as 3.4 and contained milligram-per-liter concentrations of iron, copper, aluminum and other metals. From 1984 to 1994, concentrations of contaminants in the alluvial aquifer in Pinal Creek Basin, Arizona, decreased as a result of mixing, recharge, remedial pumping and chemical reactions. For reactions involving gypsum and rhodochrosite, the equilibrium modeling assumption of a local geochemical equilibrium was generally valid. From 1984 to 1990, water along the simulated flow path was at equilibrium or slightly supersaturated with gypsum, and gypsum equilibria controlled dissolved concentrations of calcium and sulfate. Beginning in 1991, water in the acidic part of the plume became increasingly undersaturated with respect to gypsum, indicating that the gypsum available for dissolution in the aquifer may have been completely consumed by about 1991. Rhodochrosite precipitation was thought responsible for the measured attenuation in dissolved manganese in the neutralized zone. For reactions involving calcite, the assumption of a local geochemical equilibrium was generally not valid. Dissolution of calcite in the transition zone was not sufficient to establish equilibrium although, following neutralization, the calcite saturation index decreased to −1.2 in 1986. Calcite undersaturation decreased along the flow path in the neutralized zone, and equilibrium was attained about 7 km downgradient of the transition zone. The assumption of a local geochemical equilibrium was not valid for oxidation–reduction reactions that involved iron oxides and manganese oxides. Kinetically controlled oxidation–reduction reactions continued in the acidic part of the flow path for years following the passage of the transition zone. Although the equilibrium approach helped to provide an increased understanding of contaminant transport at Pinal Creek, future work will require a kinetic modeling approach to more accurately simulate selected reactions between the plume and aquifer materials.

Arizona↗

Evaluation of daily creel and minimum length limits for Black Crappies and Yellow Perch in Wisconsin

Harvest regulations for Black Crappie Pomoxis nigromaculatus and Yellow Perch Perca flavescens in the northern USA and Canada have not been thoroughly evaluated, and specific guidance regarding where minimum length limits (MLLs) might improve these fisheries is lacking. We examined whether: (1) transitioning from an aggregate statewide daily creel limit of 25 panfish to species-specific daily creel limits of <25 fish or implementing statewide MLLs could reduce harvest of Black Crappie and Yellow Perch in Wisconsin by &ge;25%, and (2) MLLs would improve yield by &ge;10% and mean TL of harvested fish by &ge;25 mm in Wisconsin fisheries. Creel surveys indicated that &ge;94% of Wisconsin anglers did not harvest a Black Crappie or Yellow Perch, and &le;0.12% of anglers harvested a daily creel limit of 25 fish. Daily creel limits would need to be &le;7 fish/ angler to reduce harvest by &ge;25%. Statewide MLLs would need to be &ge;229 mm for Black Crappie and &ge;203 mm for Yellow Perch to reduce harvest by &ge;25%, but predicted responses to MLLs varied among simulated populations. In general, MLLs were not predicted to improve yield, indicating that growth overfishing was not a widespread problem. Minimum length limits could improve mean TL of harvested fish, but increases &ge;25 mm were only observed under 254-mm and 279-mm MLLs, and anglers would have to accept predicted reductions in harvest of &ge;30% to achieve these improvements. A 229-mm MLL offered a more equitable trade-off between increases in mean TLs of harvested fish (11&ndash;21-mm improvements) and reductions in harvest (22&ndash;37% reductions). Our modeling provides a framework for managers to make more informed decisions regarding harvest regulations, but more information regarding angler preferences is needed for selecting appropriate management objectives and harvest regulations.

North American Journal of Fisheries Management↗

Evolution of CAM and C 4 carbon-concentrating mechanisms

Mechanisms for concentrating carbon around the Rubisco enzyme, which drives the carbon-reducing steps in photosynthesis, are widespread in plants; in vascular plants they are known as crassulacean acid metabolism (CAM) and C 4 photosynthesis. CAM is common in desert succulents, tropical epiphytes, and aquatic plants and is characterized by nighttime fixation of CO2. The proximal selective factor driving the evolution of this CO2-concentrating pathway is low daytime CO2, which results from the unusual reverse stomatal behavior of terrestrial CAM species or from patterns of ambient CO2 availability for aquatic CAM species. In terrestrials the ultimate selective factor is water stress that has selected for increased water use efficiency. In aquatics the ultimate selective factor is diel fluctuations in CO2 availability for palustrine species and extreme oligotrophic conditions for lacustrine species. C4 photosynthesis is based on similar biochemistry but carboxylation steps are spatially separated in the leaf rather than temporally as in CAM. This biochemical pathway is most commonly associated with a specialized leaf anatomy known as Kranz anatomy; however, there are exceptions. The ultimate selective factor driving the evolution of this pathway is excessively high photorespiration that inhibits normal C3 photosynthesis under high light and high temperature in both terrestrial and aquatic habitats. CAM is an ancient pathway that likely has been present since the Paleozoic era in aquatic species from shallow-water palustrine habitats. While atmospheric CO2 levels have undoubtedly affected the evolution of terrestrial plant carbon-concentrating mechanisms, there is reason to believe that past atmospheric changes have not played as important a selective role in the aquatic milieu since palustrine habitats today are not generally carbon sinks, and the selective factors driving aquatic CAM are autogenic. Terrestrial CAM, in contrast, is of increasing selective value under extreme water deficits, and undoubtedly, high Mesozoic CO2 levels reduced the amount of landscape perceived by plants as water limited. Late Tertiary and Quaternary reductions in atmospheric CO2, coupled with increasing seasonality, were probably times of substantial species radiation and ecological expansion for CAM plants. C4 photosynthesis occurs in only about half as many families as CAM, and three-fourths of C4 species are either grasses or sedges. Molecular phylogenies indicate C4 is a more recent innovation than CAM and that it originated in the mid-Tertiary, 20–30 Ma, although some data support an earlier origin. While the timing of the origin of C4 remains controversial, the nearly explosive increase in C4 species is clearly documented in the late Miocene, 4–7 Ma. Increasing seasonality has been widely suggested as an important climatic stimulus for this C4 expansion. Alternatively, based on models of photosynthetic quantum yield at different temperatures and CO2 concentration, it has been hypothesized that the late Miocene C4 expansion resulted from declining atmospheric CO2 levels. This model is most appropriate for explaining the transition from C3 grasslands to C4 grasslands but by itself may not be sufficient to explain the more likely scenario of a late Miocene transition from C3 woodland/ savanna to C4 grasslands. A largely unexplored hypothesis is that climatic changes in late Miocene altered disturbance regimes, in particular the incidence of fires, which today are often associated with maintenance of C4 grasslands. Oceanic charcoal sediments that appear to represent Aeolian deposits from continental wildfires follow a strikingly similar pattern of explosive increase in late Miocene. Climate, CO2, and disturbance are not mutually exclusive explanations and probably all acted in concert to promote the expansion of C4 grasslands. More recently, late Quaternary changes in CO2 may have been responsible for driving major changes in the landscape distribution of C4 species. The theory is sound; however, many of the studies cited in support of this model are open to alternative interpretations, and none has eliminated climatic factors as important selective agents. CAM and C4 evolution required coupling of biochemical pathways with structural changes in photosynthetic tissues, succulence in CAM and Kranz in C4. This was apparently accomplished by piecemeal evolution beginning with mechanisms for recapturing respiratory CO2, although this need not have been so in aquatic CAM species. It has been proposed that the extreme rarity of both pathways in the same plant results from biochemical and structural incompatibilities (Sage 2002). Equally important is the fact that the selective environments are quite different, with CAM evolution thriving on stressful sites inhospitable to C3 species whereas C4 evolution has selected for rapid growth capable of outcompeting associated C3 plants.

International Journal of Plant Sciences↗

Influence of harvesting pressure on demographic tactics: Implications for wildlife management

1. Demographic tactics within animal populations are shaped by selective pressures. Exploitation exerts additional pressures so that differing demographic tactics might be expected among populations with differences in levels of exploitation. Yet little has been done so far to assess the possible consequences of exploitation on the demographic tactics of mammals, even though such information could influence the choice of effective management strategies. 2. Compared with similar‐sized ungulate species, wild boar Sus scrofa has high reproductive capabilities, which complicates population management. Using a perturbation analysis, we investigated how population growth rates (λ) and critical life‐history stages differed between two wild boar populations monitored for several years, one of which was heavily harvested and the other lightly harvested. 3. Asymptotic λ was 1·242 in the lightly hunted population and 1·115 in the heavily hunted population, while the ratio between the elasticity of adult survival and juvenile survival was 2·63 and 1·27, respectively. A comparative analysis including 21 other ungulate species showed that the elasticity ratio in the heavily hunted population was the lowest ever observed. 4. Compared with expected generation times of similar‐sized ungulates (more than 6 years), wild boar has a fast life‐history speed, especially when facing high hunting pressure. This is well illustrated by our results, where generation times were 3·6 years in the lightly hunted population and only 2·3 years in the heavily hunted population. High human‐induced mortality combined with non‐limiting food resources accounted for the accelerated life history of the hunted population because of earlier reproduction. 5. Synthesis and applications . For wild boar, we show that when a population is facing a high hunting pressure, increasing the mortality in only one age‐class (e.g. adults or juveniles) may not allow managers to limit population growth. We suggest that simulations of management strategies based on context‐specific demographic models are useful for selecting interventions for population control. This type of approach allows the assessment of population response to exploitation by considering a range of plausible scenarios, improving the chance of selecting appropriate management actions.

Journal of Applied Ecology↗

Improving regional-model estimates of urban-runoff quality using local data

Urban water-quality managers need load estimates of storm-runoff pollutants to design effective remedial programs. Estimates are commonly made using published models calibrated to large regions of the country. This paper presents statistical methods, termed model-adjustment procedures (MAPs), which use a combination of local data and published regional models to improve estimates of urban-runoff quality. Each MAP is a form of regression analysis that uses a local data base as a calibration data set to adjust the regional model, in effect increasing the size of the local data base without additional, expensive data collection. The adjusted regional model can then be used to estimate storm-runoff quality at unmonitored sites and storms in the locality. The four MAPs presented in this study are (1) single-factor regression against the regional model prediction, P u ; (2) least-squares regression against P u ; (3) least-squares regression against P u and additional local variables; and (4) weighted combination of P u and a local-regression prediction. Identification of the statistically most valid method among these four depends upon characteristics of the local data base. A MAP-selection scheme based on statistical analysis of the calibration data set is presented and tested.

Journal of the American Water Resources Associatio↗

Behavioral responses of sea lamprey (Petromyzon marinus) and white sucker (Catostomus commersonii) to turbulent flow during fishway passage attempts

An understanding of how undesirable and desirable fish species respond behaviorally to turbulent flow in fishways would guide development of selective fish passage techniques. We applied high-resolution computational fluid dynamics modeling and competing risks analysis towards the development of predictive selective passage models. Sea lamprey ( Petromyzon marinus ; an invasive fish in the Great Lakes Basin, North America) upstream passage probability declined from 0.73 to 0.03 as flow conditions became increasingly turbulent, while declines in white sucker ( Catostomus commersonii , a native fish in the region) upstream passage probability were less substantial (0.53 to 0.44). Deploying a sea lamprey trap in the fishway did not effectively reduce sea lamprey upstream passage probability, though capture rate increased during trials with cooler water temperature and low total kinetic energy. Bifurcated fishways that maintain low turbulent flow in the entrapment route and high turbulent flow in the upstream passage route could increase the effectiveness of trapping sea lamprey in fishways as a means to advance selective passage goals.

Wisconsin↗

Selection of spawning sites by coho salmon in a northern California stream

We assessed the relative importance of various factors contributing to spawning site use by a population of threatened coho salmon Oncorhynchus kisutch in Freshwater Creek, California, and created a predictive model of spawning habitat selection based on logistic regression analysis. We excluded sampling sites that previous studies had established as unsuitable on the basis of depth and substrate criteria and asked why fish chose particular locations and not others in seemingly suitable habitat. We evaluated surface water velocity, depth, substrate size composition, gravel inflow rates, vertical hydraulic gradient, geomorphic channel units, hyporheic water physicochemistry, cover, and proximity to other redds not in sampling sites during the 2004-2005 spawning season. In univariate comparisons with unused sites, coho salmon selected sites with a smaller median particle diameter, a larger percentage of gravel-pebble substrate, and higher gravel inflow rates. Based on multivariate logistic regression, the probability of a site's being used for spawning was best modeled as a positive function of the gravel-pebble fraction of the substrate, location at a pool or run tail, and the presence of existing redds in close proximity to the site. This model explained 38% of the variation in the data and was a better predictor of spawning habitat use than a more traditional model based on depth, velocity, and substrate. Our results highlight the potential importance of social behavior in contributing to habitat selection by spawning salmonids. ?? Copyright by the American Fisheries Society 2007.

North American Journal of Fisheries Management↗

Evaluation of methods for identifying spawning sites and habitat selection for alosines

Characterization of riverine spawning habitat is important for the management and restoration of anadromous alosines. We examined the relative effectiveness of oblique plankton tows and spawning pads for collecting the eggs of American shad Alosa sapidissima , hickory shad A. mediocris , and &ldquo;river herring&rdquo; (a collective term for alewife A. pseudoharengus and blueback herring A. aestivalis ) in the Roanoke River, North Carolina. Relatively nonadhesive American shad eggs were only collected by plankton tows, whereas semiadhesive hickory shad and river herring eggs were collected by both methods. Compared with spawning pads, oblique plankton tows had higher probabilities of collecting eggs and led to the identification of longer spawning periods. In assumed spawning areas, twice-weekly plankton sampling for 15 min throughout the spawning season had a 95% or greater probability of collecting at least one egg for all alosines; however, the probabilities were lower in areas with more limited spawning. Comparisons of plankton tows, spawning pads, and two other methods of identifying spawning habitat (direct observation of spawning and examination of female histology) suggested differences in effectiveness and efficiency. Riverwide information on spawning sites and timing for all alosines is most efficiently obtained by plankton sampling. Spawning pads and direct observations of spawning are the best ways to determine microhabitat selectivity for appropriate species, especially when spawning sites have previously been identified. Histological examination can help determine primary spawning sites but is most useful when information on reproductive biology and spawning periodicity is also desired. The target species, riverine habitat conditions, and research goals should be considered when selecting methods with which to evaluate alosine spawning habitat.

North American Journal of Fisheries Management↗

An attempt to age mallards using eye lens proteins

An analysis of insoluble protein content of eye lenses from 59 known-age mallards ( Anas platyrhynchos ) indicated a slight increase between 8-9 months and 7 years of age. Nearly a complete overlapping of the insoluble protein content of individuals of different ages was apparent showing that the technique cannot be used to separate adult year classes of mallards. These results are contrary to findings reported for selected mammalian species; a possible explanation for the dissimilarity is discussed.

Journal of Wildlife Management↗

Mass-balance model for predicting nitrate in ground water

A mass-balance accounting model can be used to guide the management of septic systems and fertilizers to control the degradation of ground-water quality in zones of an aquifer that contribute water to public-supply wells. The nitrate concentration of the mixture in the well can be predicted for steady-state conditions by calculating the concentration that results from the total weight of nitrogen and total volume of water entering the zone of contribution to the well. These calculations will allow water-quality managers to predict the nitrate concentrations that would be produced by different types and levels of development, and to plan development accordingly. Computations for different development schemes provide a technical basis for planners and managers to compare water-quality effects and to select alternatives that limit nitrate concentration in wells.

Journal of the New England Water Works Association↗