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At least 109 records · Page 6Linked to original sources

Adsorption to fish sperm of vertically transmitted fish viruses

More than 99 percent of a vertically transmitted fish rhabdovirus, infectious hematopoietic necrosis virus, was removed from suspension in less than 1 minute by adsorption to the surface membrane of sperm from two genera of salmonid fishes. The vertically transmitted, infectious pancreatic necrosis virus adsorbed to a lesser degree, but no adsorption occurred with a second fish rhabdovirus that is not vertically transmitted. Such adsorption may be involved in vertical transmission of these viruses.

Science

Identifying when tagged fishes have been consumed by piscivorous predators: application of multivariate mixture models to movement parameters of telemetered fishes

Background Consumption of telemetered fishes by piscivores is problematic for telemetry studies because tag detections from the piscivore could introduce bias into the analysis of telemetry data. We illustrate the use of multivariate mixture models to estimate group membership (smolt or predator) of telemetered juvenile Chinook salmon ( Oncorhynchus tshawytscha ), juvenile steelhead trout ( O. mykiss ), striped bass ( Morone saxatilis ), smallmouth bass ( Micropterus dolomieu ) and spotted bass ( M. punctulatus ) in the Sacramento River, CA, USA. First, we estimated two types of track statistics from spatially explicit two-dimensional movement tracks of telemetered fishes: the Lévy exponent ( b ) and tortuosity ( τ ). Second, we hypothesized that the distribution of each track statistic would differ between predators and smolts. To estimate the distribution of track statistics for putative predators and smolts, we fitted a bivariate normal mixture model to the mixed distribution of track statistics. Lastly, we classified each track as a smolt or predator using parameter estimates from the mixture model to estimate the probability that each track was that of a predator or smolt. Results Tracks classified as predators exhibited movement that was tortuous and consistent with prey searching tactics, whereas tracks classified as smolts were characterized by directed, linear downstream movement. The estimated mean tortuosity was 0.565 (SD = 0.07) for predators and 0.944 (SD = 0.001) for smolts. The estimated mean Lévy exponent was 1.84 (SD = 1.23) for predators and -0.304 (SD = 1.46) for smolts. We correctly classified 90% of the Micropterus species and 72% of the striped bass as predators. For tagged smolts, 80% of Chinook salmon and 74% of steelhead trout were not classified as predators. Conclusions Mixture models proved valuable as a means to differentiate between salmonid smolts and predators that consumed salmonid smolts. However, successful application of this method requires that telemetered fishes and their predators exhibit measurable differences in movement behavior. Our approach is flexible, allows inclusion of multiple track statistics and improves upon rule-based manual classification methods.

California

Prey fish returned to Forster’s tern colonies suggest spatial and temporal differences in fish composition and availability

Predators sample the available prey community when foraging; thus, changes in the environment may be reflected by changes in predator diet and foraging preferences. We examined Forster’s tern ( Sterna forsteri ) prey species over an 11-year period by sampling approximately 10,000 prey fish returned to 17 breeding colonies in south San Francisco Bay, California. We compared the species composition among repeatedly-sampled colonies (≥ 4 years), using both relative species abundance and the composition of total dry mass by species. Overall, the relative abundances of prey species at seven repeatedly-sampled tern colonies were more different than would be expected by chance, with the most notable differences in relative abundance observed between geographically distant colonies. In general, Mississippi silverside ( Menidia audens ) and topsmelt silverside ( Atherinops affinis ) comprised 42% of individuals and 40% of dry fish mass over the study period. Three-spined stickleback ( Gasterosteus aculeatus ) comprised the next largest proportion of prey species by individuals (19%) but not by dry mass (6%). Five additional species each contributed ≥ 4% of total individuals collected over the study period: yellowfin goby ( Acanthogobius flavimanus ; 10%), longjaw mudsucker ( Gillichthys mirabilis ; 8%), Pacific herring ( Clupea pallasii ; 6%), northern anchovy ( Engraulis mordax ; 4%), and staghorn sculpin ( Leptocottus armatus ; 4%). At some colonies, the relative abundance and biomass of specific prey species changed over time. In general, the abundance and dry mass of silversides increased, whereas the abundance and dry mass of three-spined stickleback and longjaw mudsucker decreased. As central place foragers, Forster’s terns are limited in the distance they forage; thus, changes in the prey species returned to Forster’s tern colonies suggest that the relative availability of some fish species in the environment has changed, possibly in response to alteration of the available habitat.

California

A disease of freshwater fishes caused by Tetrahymena corlissi Thompson, 1955, and a key for identification of holotrich ciliates of freshwater fishes

Tetrahymena corlissi , a free-living protozoan, apparently caused the death of large numbers of guppies ( Poecilia reticulatus ) and occasionally other fishes, in aquaria and hatcheries at several locations. Apparently the disease occurs when the fish and protozoan populations are both at a high level of density. The signs include white spots and epidermal damage. Histologically, T. corlissi could be seen in skin, muscle, and viscera; in some there was marked inflammation, in others there was little tissue reaction. A key is presented for the identification of invasive fish ciliates: Chilodonella , Hemiophrys , Ichthyophthirius , Ophryoglena , and Tetrahymena .

Journal of Parasitology

Examination of native fish recruitment and description of the fish communities found in the San Juan and Colorado River interface zones of Lake Powell, Utah

Researchers examined the fish communities of the Colorado and San Juan river interface zones of Lake Powell during 1999 and 2000. The objectives were to: (1) search for young razorback sucker and Colorado pikeminnow and, if found, RIT tag them; (2) examine the effectiveness of the various collection techniques on juvenile native fish; and (3) describe the fish communities found in these transitory, or actually a??migratorya?? habitats. The San Juan River interface zone was sampled 5 time each year during the spring and summer while the Colorado River site was sampled a total of 3 times over the 2-year period, all in the springa?|

Open-File Report

Mycobacteria in adult salmonid fishes returning to national fish hatcheries in Washington, Oregon and California in 1958-59

Incidence of acid-fast bacillus infections in salmonid fishes at West Coast hatcheries was determined for 1957-59. No evidence was obtained which would indicate a definite trend towards either increased or decreased rates of infection. It is apparent that the incidence of infection is higher in hatchery-marked fish than in unmarked fish. Only one hatchery was found free of infection during the 3 years covered by the investigation. This installation had never used raw salmon products in diet.

California, Oregon

Habitat use by an endangered riverine fish and implications for species protection

We investigated habitat specificity of the amber darter ( Percina antesella Williams & Etnier 1977), an imperiled fish from restricted portions of 2 rivers in the southeastern United States. Foraging amber darters occupied a narrow range of riffle habitat, consistently avoiding areas < 20 cm deep and with velocity < 10 cm. s −1 near the substrate, occupying areas with cobble or gravel substrate and average water-column velocity of 30 to 70 cm. s −1 . During low to moderate flows, approximately 20% or more of the study areas contained suitable habitat for the species. Amber darters appeared rare, and the numbers of individuals were uncorrelated with the concurrent availability of suitable habitat. Protecting the amber darter may require more than maintaining adequate depths and velocities over gravel-cobble substrates. Until we understand the potential importance of migration and dispersal for maintaining small populations, suitable habitat should be maintained over the longest contiguous stream segments possible.

southeastern United States

Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish

Gut microbiota of invasive Asian silver carp (SVCP) and indigenous planktivorous gizzard shad (GZSD) in Mississippi river basin were compared using 16S rRNA gene pyrosequencing. Analysis of more than 440 000 quality-filtered sequences obtained from the foregut and hindgut of GZSD and SVCP revealed high microbial diversity in these samples. GZSD hindgut (GZSD_H) samples (n=23) with >7000 operational taxonomy units (OTUs) exhibited the highest alpha-diversity indices followed by SVCP foregut (n=15), GZSD foregut (n=9) and SVCP hindgut (SVCP_H) (n=24). UniFrac distance-based non-metric multidimensional scaling (NMDS) analysis showed that the microbiota of GZSD_H and SVCP_H were clearly separated into two clusters: samples in the GZSD cluster were observed to vary by sampling location and samples in the SVCP cluster by sampling date. NMDS further revealed distinct microbial community between foregut to hindgut for individual GZSD and SVCP. Cyanobacteria, Proteobacteria, Actinobacteria and Bacteroidetes were detected as the predominant phyla regardless of fish or gut type. The high abundance of Cyanobacteria observed was possibly supported by their role as the fish’s major food source. Furthermore, unique and shared OTUs and OTUs in each gut type were identified, three OTUs from the order Bacteroidales, the genus Bacillariophyta and the genus Clostridium were found significantly more abundant in GZSD_H (14.9–22.8%) than in SVCP_H (0.13–4.1%) samples. These differences were presumably caused by the differences in the type of food sources including bacteria ingested, the gut morphology and digestion, and the physiological behavior between GZSD and SVCP.

International Society for Microbial Ecology

Effectiveness of eugenol sedation to reduce the metabolic rates of cool and warm water fish at high loading densities

Effects of eugenol (AQUI-S &reg; 20E, 10% active eugenol) sedation on cool water, yellow perch Perca flavescens (Mitchill), and warm water, Nile tilapia Oreochromis niloticus L. fish metabolic rates were assessed. Both species were exposed to 0, 10, 20 and 30 mg L &minus;1 eugenol using static respirometry. In 17&deg;C water and loading densities of 60, 120 and 240 g L &minus;1 , yellow perch controls (0 mg L &minus;1 eugenol) had metabolic rates of 329.6&ndash;400.0 mg O 2 kg &minus;1 h &minus;1 , while yellow perch exposed to 20 and 30 mg L &minus;1 eugenol had significantly reduced metabolic rates of 258.4&ndash;325.6 and 189.1&ndash;271.0 mg O 2 kg &minus;1 h &minus;1 respectively. Nile tilapia exposed to 30 mg L &minus;1 eugenol had a significantly reduced metabolic rate (424.5 &plusmn; 42.3 mg O 2 kg &minus;1 h &minus;1 ) relative to the 0 mg L &minus;1 eugenol control (546.6 &plusmn; 53.5 mg O 2 kg &minus;1 h &minus;1 ) at a loading density of 120 g L &minus;1 in 22&deg;C water. No significant differences in metabolic rates for Nile tilapia were found at 240 or 360 g L &minus;1 loading densities when exposed to eugenol. Results suggest that eugenol sedation may benefit yellow perch welfare at high densities (e.g. live transport) due to a reduction in metabolic rates, while further research is needed to assess the benefits of eugenol sedation on Nile tilapia at high loading densities.

Aquaculture Research

A combined telemetry - tag return approach to estimate fishing and natural mortality rates of an estuarine fish

A joint analysis of tag return and telemetry data should improve estimates of mortality rates for exploited fishes; however, the combined approach has thus far only been tested in terrestrial systems. We tagged subadult red drum (Sciaenops ocellatus) with conventional tags and ultrasonic transmitters over 3 years in coastal North Carolina, USA, to test the efficacy of the combined telemetry - tag return approach. There was a strong seasonal pattern to monthly fishing mortality rate (F) estimates from both conventional and telemetry tags; highest F values occurred in fall months and lowest levels occurred during winter. Although monthly F values were similar in pattern and magnitude between conventional tagging and telemetry, information on F in the combined model came primarily from conventional tags. The estimated natural mortality rate (M) in the combined model was low (estimated annual rate ?? standard error: 0.04 ?? 0.04) and was based primarily upon the telemetry approach. Using high-reward tagging, we estimated different tag reporting rates for state agency and university tagging programs. The combined telemetry - tag return approach can be an effective approach for estimating F and M as long as several key assumptions of the model are met.

Canadian Journal of Fisheries and Aquatic Sciences

Revealing the appetite of the marine aquarium fish trade: the volume and biodiversity of fish imported into the United States

The aquarium trade and other wildlife consumers are at a crossroads forced by threats from global climate change and other anthropogenic stressors that have weakened coastal ecosystems. While the wildlife trade may put additional stress on coral reefs, it brings income into impoverished parts of the world and may stimulate interest in marine conservation. To better understand the influence of the trade, we must first be able to quantify coral reef fauna moving through it. Herein, we discuss the lack of a data system for monitoring the wildlife aquarium trade and analyze problems that arise when trying to monitor the trade using a system not specifically designed for this purpose. To do this, we examined an entire year of import records of marine tropical fish entering the United States in detail, and discuss the relationship between trade volume, biodiversity and introduction of non-native marine fishes. Our analyses showed that biodiversity levels are higher than previous estimates. Additionally, more than half of government importation forms have numerical or other reporting discrepancies resulting in the overestimation of trade volumes by 27%. While some commonly imported species have been introduced into the coastal waters of the USA (as expected), we also found that some uncommon species in the trade have also been introduced. This is the first study of aquarium trade imports to compare commercial invoices to government forms and provides a means to, routinely and in real time, examine the biodiversity of the trade in coral reef wildlife species.

PLoS ONE

Evaluation of commercially prepared transport systems for nonlethal detection of Aeromonas salmonicida in salmonid fish

In vitro studies indicated that commercially prepared transport systems containing Amies, Stuart's, and Cary-Blair media worked equally well in sustaining the viability of the fish pathogen Aeromonas salmonicida, which causes furunculosis. The bacterium remained viable without significant increase or decrease in cell numbers for as long as 48 h of incubation at 18-20??C in Stuart's transport medium; consequently, obtaining mucus samples in such tubes were comparable to on-site detection of A. salmonicida by dilution plate counts on Coomassie Brilliant Blue agar. In three different assays of 100 samples of mucus from Atlantic salmon Salmo salar infected subclinically with A. salmonicida, dilution counts conducted on-site proved more reliable for detecting the pathogen than obtaining the samples in the transport system. In the on-site assays, dilution counts detected the pathogen in 34, 41, and 22 samples, whereas this was accomplished in only 15, 15, and 3 of the respective samples when the transport system was used. In an additional experiment, Arctic char Salvelinus alpinus sustaining a frank epizootic of furunculosis were sampled similarly. Here, too, dilution counts were more predictive of the prevalence of A. salmonicida and detected the pathogen in 46 mucus samples; in comparison, only 6 samples collected by using the transport system were positive. We also observed that the transport system supported the growth of the normal mucus bacterial flora. Particularly predominant among these were motile aeromonads and Pseudomonas fluorescens. In studies of mixed culture growth, two representatives of both of the latter genera of bacteria outgrew A. salmonicida - in some cases, to the total exclusion of the pathogen itself.

Journal of Aquatic Animal Health

Can diet-dependent factors help explain fish-to-fish variation in thiamine-dependent early mortality syndrome?

To provide insight into the reasons why offspring of certain salmonine females exhibit early mortality syndrome (EMS) in the Great Lakes whereas others do not, we measured the egg concentrations of potential biochemical markers (stable isotopes of nitrogen and carbon, fatty acid signatures, and lipid-soluble carotenoids and vitamins) that are indicative of differing food web and trophic structure. To corroborate the presence of EMS, we also measured the egg content of thiamine vitamers. For all the stocks of coho salmon Oncorhynchus kisutch and Chinook salmon O. tshawytscha we studied, there was a very high correspondence between EMS and low concentrations of unphosphorylated thiamine in unfertilized eggs. For salmonine stocks in the Platte River, Thompson Creek, and the Swan River, Michigan, small but significant shifts occurred in measures of egg carotenoids, retinoids, ??15N depletion, and fatty acid profiles of fish producing normal offspring relative to those exhibiting EMS. Egg thiamine concentrations in Chinook salmon from the Little Manistee River, Michigan, in the low-EMS group were only marginally above the threshold for EMS induction. Along with this small thiamine differential, there was no evidence of differing food web or dietary factors between EMS-positive and normal Chinook salmon from the Little Manistee River. Further investigations are required to determine the potential dietary sources for the observed differences in biochemical markers between EMS-positive and normal fish. These findings are generally consistent with the hypothesis that a more diverse forage base may help to limit overall dietary content of species that contain thiaminase, such as alewives Alosa pseudoharengus, and may lead to improved embryonic survival for feral salmonids. ?? Copyright by the American Fisheries Society 2005.

Journal of Aquatic Animal Health

Endangered fish threatened by Asian fish tapeworm

The Asian fish tapeworm, an exotic parasite, has invaded the endangered humpback chub ( Gila cypha ) population from the Colorado and Little Colorado Rivers in Grand Canyon, Arizona. This parasite causes disease and death in carp in aquaculture settings and may retard growth in hatchery-reared roundtail chub ( Gila robusta) . Other consequences include destruction and dysfunction of the intestinal lining and adverse changes to certain blood parameters. Introduced into the U.S. in the 1970s with imported grass carp ( Ctenopharyngodon idella ), the Asian fish tapeworm ( Bothriocephalus acheilognathi ) was discovered in the Little Colorado River (LCR) by 1990. The LCR is the main tributary to the Colorado River in Grand Canyon and is an important spawning area for humpback chub.

Fact Sheet

PCR-based assays for the fish pathogen Aeromonas salmonicida: Further evaluation and validation of three PCR primer sets with infected fish

Two Aeromonas salmonicida -specific polymerase chain reaction (PCR) tests and 1 A. salmonicida subsp. salmonicida -specific PCR test were used to screen salmonid populations that were either overtly or covertly infected with A. salmonicida subsp. salmonicida . It was demonstrated that these PCR assays could be used to replace the biochemical testing currently employed to confirm the identity of A. salmonicida isolates cultured from infected fish. The AP and PAAS PCR assays were also capable of direct detection of A. salmonicida in overtly infected fish, with mucus, gill and kidney samples most likely to yield a positive result. Culture was a more reliable method for the direct detection of A. salmonicida in covertly infected salmonids than was the direct PCR testing of tissue samples, with the AP and PAAS PCRs having a lower detection limit (LDL) of approximately 4 x 10 5 colony-forming units (CFU) g -1 sample.

Diseases of Aquatic Organisms

The role of reproductive behavior in the conservation of fishes: examples from the Great Plains riverine fishes

Recovery efforts for threatened and endangered fish species are hampered by lack of knowledge of their reproductive ecology. Habitat requirements and environmental stimuli necessary for reproduction are often unknown and vary widely among species. For Great Plains riverine fishes, this is often complicated by the high turbidity of the system in which the species occur, which precludes direct visual observation of behavior. Innovative methods for collectng behavioral data are required to better understand the conditions necessary for successful reproduction.

American Currents

Evaluation of the fish passage effectiveness of the Bonneville I prototype surface collector using three-dimensional ultrasonic fish tracking - Final Report

This report describes tests conducted at Bonneville Dam on the Columbia River in the spring of 2000. The studies used three-dimensional (3D) acoustic telemetry and computational fluid dynamics (CFD) hydraulic modeling techniques to evaluate the response of outmigrating juvenile steelhead (Oncorhynchus mykiss) and yearling chinook (O. tshawytscha) to the Prototype Surface Collector (PSC) installed at Powerhouse I of Bonneville Dam in 1998 to test the concept of using a deep-slot surface bypass collector to divert downstream migrating salmon from turbines. The study was conducted by Pacific Northwest National Laboratory (PNNL), the Waterways Experiment Station of the U.S. Army Corp of Engineers (COE), Asci Corporation, and the U.S. Geological Survey (USGS), and was sponsored by COE’s Portland District. The goal of the study was to observe the three-dimensional behavior of tagged fish (fish bearing ultrasonic micro-transmitters) within 100 meters (m) of the surface flow bypass structure to test hypotheses about the response of migrants to flow stimuli generated by the presence of the surface flow bypass prototype and its operation. Research was done in parallel with radio telemetry studies conducted by USGS and hydroacoustic studies conducted by WES & Asci to evaluate the prototype surface collector.

Report