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Biomonitoring of Environmental Status and Trends (BEST) Program: Environmental contaminants, health indicators, and reproductive biomarkers in fish from the Colorado River basin

Seven fish species were collected from 14 sites on rivers in the Colorado River Basin (CDRB) from August to October 2003. Spatial trends in the concentrations of accumulative contaminants were documented and contaminant effects on the fish were assessed. Sites were located on the mainstem of the Colorado River and on the Yampa, Green, Gunnison, San Juan, and Gila Rivers. Common carp (Cyprinus carpio), black bass (Micropterus sp.), and channel catfish (Ictalurus punctatus) were the targeted species. Fish were field-examined for external and internal anomalies, selected organs were weighed to compute somatic indices, and tissue and fluid samples were preserved for fish health and reproductive biomarker analyses. Composite samples of whole fish, grouped by species and gender, from each site were analyzed for organochlorine and elemental contaminants using performance-based and instrumental methods. 2,3,7,8-tetrachlorodibenzo-p-dioxin-like activity (TCDD-EQ) was measured using the H4IIE rat hepatoma cell bioassay. Selenium (Se) and mercury (Hg) concentrations were elevated throughout the CDRB, and pesticides concentrations were greatest in fish from agricultural areas in the Lower Colorado River and Gila River. Selenium concentrations exceeded toxicity thresholds for fish (>1.0 ?g/g ww) at all sites except from the Gila River at Hayden, Arizona. Mercury concentrations were elevated (>0.1 ?g/g ww) in fish from the Yampa River at Lay, Colorado; the Green River at Ouray National Wildlife Refuge (NWR), Utah and San Rafael, Utah; the San Juan River at Hogback Diversion, New Mexico; and the Colorado River at Gold Bar Canyon, Utah, Needles, California, and Imperial Dam, Arizona. Concentrations of p,p'-DDE were relatively high in fish from Arlington, Arizona (>1.0 ?g/g ww) and Phoenix, Arizona (>0.5 ?g/g ww). Concentrations of other banned pesticides including toxaphene, total chlordanes, and dieldrin were also greatest at these two sites but did not exceed toxicity thresholds. Current-use or unlisted pesticides such as dacthal, endosulfan, '-HCH, and methoxychlor were also greatest in fish from Gila River. Total polychlorinated biphenyls (PCBs; >0.11 ?g/g ww) and TCDD-EQs (>5 pg/g ww) exceeded wildlife guidelines in fish from the Gila River at Phoenix, Arizona. Hepatic ethoxyresorufin O-deethylase (EROD) activity was also relatively high in carp from the Gila River at Phoenix, Arizona and in bass from the Green River at Ouray NWR, Utah. Altered biomarkers were noted in fish throughout the CDRB. Fish from some stations responded to chronic contaminant exposure as indicated by fish health and reproductive biomarker results. Multiple fish health indicators including altered body and organ weights and high health assessment index scores may be associated with elevated Se concentrations in fish from the Colorado River at Loma, Colorado and Needles, California. Although grossly visible external or internal lesions were found on most fish from some sites, histopathological analysis determined many of these to be inflammatory responses associated with parasites. Edema, exophthalmos, and cataracts were noted in fish from sites with elevated Se concentrations. Reproductive biomarkers including gonad development and maturation, vitellogenin concentrations, and steroid hormone concentrations were anomalous in fish from the Gila River at Hayden and Phoenix, Arizona. In addition, intersex fish were found at seven of 14 sites. The intersex condition was identified in smallmouth bass (M. dolomieu), largemouth bass (M. salmoides), channel catfish, and carp and may indicate exposure to endocrine disrupting compounds. Seven of ten male smallmouth bass from the Yampa River at Lay, Colorado were intersex. Male carp, bass, and channel catfish with low concentrations of vitellogenin were common in the CDRB. Comparatively high vitellogenin concentrations (>0.2 mg/mL) were measured in male fish from the Green River at Ouray NWR, Utah and the Colorado River at Im

Scientific Investigations Report↗

Land-Use Change Trends in the Interior Lowland Ecoregion

This report describes land-cover trends in the Interior River Lowland ecoregion, located primarily in southern Illinois and includes the confluence areas of the Mississippi, Missouri, Ohio, Illinois, and Wabash Rivers, and their tributaries. Land-cover change statistics were tabulated from forty 10 kilometers (km) by 10-km multi-spectral remote-sensing sample areas collected from 1973 to 2000 and classified into nine primary land-cover categories. The results indicated stable land use. Agricultural land use increased, but acreage was lost to urbanization, especially in the St. Louis area. Recreational and conservation land uses are underrepresented relative to population demand. Findings were comparable to results of other land use research.

Scientific Investigations Report↗

Biomonitoring of Environmental Status and Trends (BEST) Program: Environmental contaminants, health indicators, and reproductive biomarkers in fish from the Mobile, Apalachicola-Chattahoochee-Flint, Savannah, and Pee Dee River Basins

Largemouth bass ( Micropterus salmoides ) and common carp ( Cyprinus carpio ) were collected from 13 sites in 4 river basins in the southeastern United States to document spatial trends in accumulative contaminants, health indicators, and reproductive biomarkers. Organochlorine residues, 2,3,7,8- tetrachlorodibenzo-p-dioxin-like activity (TCDD-EQ), and elemental contaminants were measured in composite samples of whole fish, grouped by species and gender, from each site. Fish were field-examined for external and internal anomalies, selected organs were weighed to compute somatic indices, and tissue and fluid samples were preserved for fish health and reproductive biomarker analyses. Mercury concentrations in bass samples from all sites exceeded toxicity thresholds for mammals [>0.1 micrograms per gram wet weight (ug/g ww)], fish (>0.2 ug/g ww), and birds (>0.3 ug/g ww) and were greatest (>0.5 ug/g ww) in samples from the Alabama River at Eureka Landing, Alabama; the Mobile River at Bucks, Alabama; the Apalachicola River at Blountstown, Florida; the Savannah River at Sylvania, Georgia; and the Pee Dee River at Bucksport, South Carolina. Selenium concentrations were relatively high (>0.75 ug/g ww) in fish from the Tombigbee River at Lavaca, Alabama; the Mobile River at Bucks; and the Chattahoochee River at Omaha, Georgia compared to those from other sites. Concentrations of 2,2-bis (p-chlorophenyl)- 1,1-dichloroethylene (p,p'-DDE) were high in fish from the Chattahoochee River at Omaha and the Mobile River near Bucks, which was near a 2,2-bis (p-chlorophenyl)-1,1- dichloroethylene (DDT) formulating facility that historically discharged into the lower Mobile River. Toxaphene concentrations in fish from the Flint River near Albany, Georgia (60-100 nanograms per gram (ng/g) ww) may pose a risk to fish. Concentrations of other formerly used (total chlordanes, dieldrin, endrin, aldrin, mirex, and hexachlorobenzene) and currently used (pentachlorobenzene, pentachloroanisole, dacthal, endosulfan, y-HCH, and methoxychlor) organochlorine residues generally were low or did not exceed toxicity thresholds. Total polychlorinated biphenyls concentrations in samples from the Coosa River at Childersburg, Alabama; the Apalachicola River at Omaha; the Apalachicola River at Blountstown; and the Pee Dee River at Bucksport were >480 ng/g ww and may be a risk to piscivorous wildlife. Dioxin-like activity as measured by TCDD-EQ was greatest [>10 picograms per gram (pg/g)] in male fish from the Coosa River at Childersburg and the Mobile River at Bucks. Hepatic ethoxyresorufin O-deethylase activity generally was greatest in carp from the Mobile River Basin [means >10 picomols per minute per milligram of protein (pmol/min/mg)] and in bass from the Tombigbee River at Lavaca and Pee Dee River at Pee Dee, South Carolina (means >65 pmol/min/mg). Altered biomarkers were noted in fish from all basins. The field necropsy and histopathological examination determined that fish from the Mobile River Basin generally were in poorer health than those from the other basins. In bass, health assessment index scores were correlated with mercury and p,p'-DDE concentrations. High health assessment index scores in Mobile River Basin fish were widespread and caused primarily by parasitic infestations, which were most severe in fish from the Tombigbee River at Lavaca and the Alabama River at Eureka Landing. Tumors were present in few fish (n = 5; 0.01%). Ovarian tumors of the same origin (smooth muscle) were present in two older carp from the Chattahoochee River near Omaha, Georgia and may be contaminant related. Reproductive biomarkers including gonadosomatic index, vitellogenin concentrations, and steroid hormone concentrations were anomalous in fish from various sites but were not consistently related to any particular chemical contaminant. Intersex gonads were identified in 47 male bass (42%) representing 12 sites and may indicate exposure to endocrine disrupting comp

Mobile, Apalachicola-Chattahoochee-Flint, Savannah↗

Land-Cover Trends of the Southern California Mountains Ecoregion

This report presents an assessment of land-use and land-cover (LU/LC) change in the Southern California Mountains ecoregion for the period 1973-2001. The Southern California Mountains is one of 84 Level-III ecoregions as defined by the U.S. Environmental Protection Agency (EPA). Ecoregions have served as a spatial framework for environmental resource management, denoting areas that contain a geographically distinct assemblage of biotic and abiotic phenomena including geology, physiography, vegetation, climate, soils, land use, wildlife, and hydrology. The established Land Cover Trends methodology generates estimates of change for ecoregions using a probability sampling approach and change-detection analysis of thematic land-cover images derived from Landsat satellite imagery.

Scientific Investigations Report↗

Character and Trends of Water Quality in the Blue River Basin, Kansas City Metropolitan Area, Missouri and Kansas, 1998 through 2007

Water-quality and ecological character and trends in the metropolitan Blue River Basin were evaluated from 1998 through 2007 to provide spatial and temporal resolution to factors that affect the quality of water and biota in the basin and provide a basis for assessing the efficacy of long-term combined sewer control and basin management plans. Assessments included measurements of stream discharge, pH, dissolved oxygen, specific conductance, turbidity, nutrients (dissolved and total nitrogen and phosphorus species), fecal-indicator bacteria (Escherichia coli and fecal coliform), suspended sediment, organic wastewater and pharmaceutical compounds, and sources of these compounds as well as the quality of stream biota in the basin. Because of the nature and myriad of factors that affect basin water quality, multiple strategies are needed to decrease constituent loads in streams. Strategies designed to decrease or eliminate combined sewer overflows (CSOs) would substantially reduce the annual loads of nutrients and fecal-indicator bacteria in Brush Creek, but have little effect on Blue River loadings. Nonpoint source reductions to Brush Creek could potentially have an equivalent, if not greater, effect on water quality than would CSO reductions. Nonpoint source reductions could also substantially decrease annual nutrient and bacteria loadings to the Blue River and Indian Creek. Methods designed to decrease nutrient loads originating from Blue River and Indian Creek wastewater treatment plants (WWTPs) could substantially reduce the overall nutrient load in these streams. For the main stem of the Blue River and Indian Creek, primary sources of nutrients were nonpoint source runoff and WWTPs discharges; however, the relative contribution of each source varied depending on how wet or dry the year was and the number of upstream WWTPs. On Brush Creek, approximately two-thirds of the nutrients originated from nonpoint sources and the remainder from CSOs. Nutrient assimilation processes, which reduced total nitrogen loads by approximately 13 percent and total phosphorus loads by double that amount in a 20-kilometer reach of the Blue River during three synoptic base-flow sampling events between August through September 2004 and September 2005, likely are limited to selected periods during any given year and may not substantially reduce annual nutrient loads. Bacteria densities typically increased with increasing urbanization, and bacteria loadings to the Blue River and Indian Creek were almost entirely the result of nonpoint source runoff. WWTPs contributed, on average, less than 1 percent of the bacteria to these reaches, and in areas of the Blue River that had combined sewers, CSOs contributed only minor amounts (less than 2 percent) of the total annual load in 2005. The bulk of the fecal-indicator bacteria load in Brush Creek also originated from nonpoint sources with the remainder from CSOs. From October 2002 through September 2007, estimated daily mean Escherichia coli bacteria density in upper reaches of the Blue River met the State of Missouri secondary contact criterion standard approximately 85 percent of the time. However, in lower Blue River reaches, the same threshold was exceeded approximately 45 percent of the time. The tributary with the greatest number of CSO discharge points, Brush Creek, contributed approximately 10 percent of the bacteria loads to downstream reaches. The tributary Town Fork Creek had median base-flow Escherichia coli densities that were double that of other basin sites and stormflow densities 10 times greater than those in other parts of the basin largely because approximately one-fourth of the runoff in the Town Fork Creek Basin is believed to originate in combined sewers. Genotypic source typing of bacteria indicated that more than half of the bacteria in this tributary originated from human sources with two storms contributing the bulk of all bacteria sourced as human. However, areas outsid

Scientific Investigations Report↗

Potentiometric Surfaces and Water-Level Trends in the Cockfield (Upper Claiborne) and Wilcox (Lower Wilcox) Aquifers of Southern and Northeastern Arkansas, 2009

Eocene-age sand beds near the base of the Cockfield Formation of Claiborne Group constitute the aquifer known locally as the Cockfield aquifer. Upper-Paleocene age sand beds within the lower parts of the Wilcox Group constitute the aquifer known locally as the Wilcox aquifer. In 2005, reported water withdrawals from the Cockfield aquifer in Arkansas totaled 16.1 million gallons per day, while reported water withdrawals from the Wilcox aquifer in Arkansas totaled 27.0 million gallons per day. Major withdrawals from these units were for industrial and public water supplies with lesser but locally important withdrawals for commercial, domestic, and agricultural uses. During February 2009, 56 water-level measurements were made in wells completed in the Cockfield aquifer and 57 water-level measurements were made in wells completed in the Wilcox aquifer. The results from the 2009 water-level measurements are presented in potentiometric-surface maps and in combination with previous water-level measurements. Trends in water-level change over time within the two aquifers are investigated using water-level difference maps and well hydrographs. Water-level difference maps were constructed for each aquifer using the difference between depth to water measurements made in 2003 to 2009. Well hydrographs for each aquifer were constructed for wells with 20 or more years of historical water-level data. The hydrographs were evaluated individually using linear regression to calculate the annual rise or decline in water levels, and by aggregating the regression results by county and statistically summarizing for the range, mean, and median water-level change in each county. The 2009 potentiometric surface of the Cockfield aquifer map indicates the regional direction of groundwater flow generally towards the east and southeast, except in two areas of intense groundwater withdrawals that have developed into cones of depression. The lowest water-level altitude measured was 43 feet and the highest water-level altitude measured was 351 feet. A water-level difference map was constructed from 54 wells completed in the Cockfield aquifer within Arkansas. The largest rise in water level was 14.9 feet and the largest decline was 27.4 feet. Seven wells had a rise in water level, and the remaining 47 wells had a decline in water level. Hydrographs for 33 wells completed in the Cockfield aquifer were developed. Hydrographs indicate water-level changes in individual wells ranged from rises of 0.33 feet per year to declines of 1.21 feet per year over the 20-year period (1990-2009). County summaries of the linear regression analysis indicate Cleveland and Columbia Counties have mean annual rises. Arkansas, Ashley, Bradley, Calhoun, Chicot, Desha, Drew, Lincoln, and Union Counties have mean annual declines. The potentiometric surface for the Wilcox aquifer is presented using two maps, one for a southern area and another for a northeastern area, because of the absence of water-level data in the central part of the State. The direction of groundwater flow in the southern area is generally the east, except around two cones of depression and around two mounds of elevated water levels. Water-level altitudes in the southern area range from 147 feet to 400 feet. The direction of groundwater flow in the northeastern area is generally to the south and southeast except in an area of intense groundwater withdrawals that has altered the flow to a westerly direction. Two water-level difference maps were constructed using water-level altitudes measured in 2003 to 2009 from 53 wells completed in the Wilcox aquifer within southern and northeastern Arkansas. In the southern area the largest rise in water level was 16.0 feet and the largest decline was 17.7 feet. Eight wells in the southern area had rising water levels and the remaining five wells had declining water levels. In the northeastern area, the largest rise in water level was 1.3 feet and the larg

Scientific Investigations Report↗

Water resources of Monroe County, New York, water years 2003-08: Streamflow, constituent loads, and trends in water quality

This report, the sixth in a series published since 1994, presents analyses of hydrologic data in Monroe County for the period October 2002 through September 2008. Streamflows and water quality were monitored at nine sites by the Monroe County Department of Health and the U.S. Geological Survey. Streamflow yields (flow per unit area) were highest in Northrup Creek, which had sustained flows from year-round inflow from the village of Spencerport wastewater-treatment plant and seasonal releases from the New York State Erie (Barge) Canal. Genesee River streamflow yields also were high, at least in part, as a result of higher rainfall and lower evapotranspiration rates in the upper part of the Genesee River Basin than in the other study basins. The lowest streamflow yields were measured in Honeoye Creek, which reflected a decrease in flows due to the withdrawals from Hemlock and Canadice Lakes for the city of Rochester water supply. Water samples collected at nine monitoring sites were analyzed for nutrients, chloride, sulfate, and total suspended solids. The loads of constituents, which were computed from the concentration data and the daily flows recorded at each of the monitoring sites, are estimates of the mass of the constituents that was transported in the streamflow. Annual yields (loads per unit area) also were computed to assess differences in constituent transport among the study basins. All urban sites - Allen Creek and the two downstream sites on Irondequoit Creek - had seasonally high concentrations and annual yields of chloride. Chloride loads are attributed to the application of road-deicing salts to the county's roadways and are related to population and road densities. The less-urbanized sites in the study - Genesee River, Honeoye Creek, and Oatka Creek - had relatively low concentrations and yields of chloride. The highest concentrations and yields of sulfate were measured in Black Creek, Oatka Creek, and Irondequoit Creek at Railroad Mills and are attributable to dissolution of sulfate from gypsum (calcium sulfate) deposits in Silurian shale bedrock that crops out upstream from these monitoring sites. Northrup Creek had the highest concentrations of phosphorus, orthophosphate, and nitrogen, and high yields of nitrate plus nitrite nitrogen and ammonia plus organic nitrogen. These results are attributed to discharges from the Spencerport wastewater-treatment plant (which ceased operation in June 2008), diversions from the New York State Erie (Barge) Canal, and manure and fertilizers applied to agricultural fields. Concentrations and yields of nitrate plus nitrite nitrogen also were high in Oatka Creek and Black Creek; basins with substantial agricultural land uses. Allen Creek had the second highest yield of ammonia plus organic nitrogen. Honeoye Creek, which drains a relatively undeveloped basin, had the lowest yields of nitrogen constituents. The second highest median concentrations and highest sample concentrations of phosphorus and orthophosphate, as well as the highest phosphorus yields, were measured in the Genesee River. A comparison of the yields computed for the two downstream sites on Irondequoit Creek - above Blossom Road and at Empire Boulevard - permitted an assessment of the mitigative effects of the Ellison Park wetland on constituent loads, which would otherwise be transported to Irondequoit Bay. These effects also include those provided by a flow-control structure (installed mid-way through the wetland during February 1997), which was designed to increase the dispersal and short-term detention of stormflows in the wetland. The wetland decreased yields of particulate constituents - phosphorus and ammonia plus organic nitrogen - but had little effect on the yields of dissolved constituents - chloride, sulfate, and nitrate plus nitrite nitrogen. Trends in flow-adjusted concentrations were identified at all sites for most of the nutrient constituents that were evaluated. All of the linear time tren

Scientific Investigations Report↗

Sedimentation and occurrence and trends of selected nutrients, other chemical constituents, and cyanobacteria in bottom sediment, Clinton Lake, northeast Kansas, 1977-2009

A combination of available bathymetric-survey information and bottom-sediment coring was used to investigate sedimentation and the occurrence of selected nutrients (total nitrogen and total phosphorus), organic and total carbon, 25 trace elements, cyanobacterial akinetes, and the radionuclide cesium-137 in the bottom sediment of Clinton Lake, northeast Kansas. The total estimated volume and mass of bottom sediment deposited from 1977 through 2009 in the conservation (multi-purpose) pool of the reservoir was 438 million cubic feet and 18 billion pounds, respectively. The estimated sediment volume occupied about 8 percent of the conservation-pool, water-storage capacity of the reservoir. Sedimentation in the conservation pool has occurred about 70 percent faster than originally projected at the time the reservoir was completed. Water-storage capacity in the conservation pool has been lost to sedimentation at a rate of about 0.25 percent annually. Mean annual net sediment deposition since 1977 in the conservation pool of the reservoir was estimated to be 563 million pounds per year. Mean annual net sediment yield from the Clinton Lake Basin was estimated to be 1.5 million pounds per square mile per year. Typically, the bottom sediment sampled in Clinton Lake was at least 99 percent silt and clay. The mean annual net loads of total nitrogen and total phosphorus deposited in the bottom sediment of Clinton Lake were estimated to be 1.29 million pounds per year and 556,000 pounds per year, respectively. The estimated mean annual net yields of total nitrogen and total phosphorus from the Clinton Lake Basin were 3,510 pounds per square mile per year and 1,510 pounds per square mile per year, respectively. Throughout the history of Clinton Lake, total nitrogen concentrations in the deposited sediment generally were uniform and indicated consistent inputs to the reservoir over time. Likewise, total phosphorus concentrations in the deposited sediment generally were uniform. Although, for two of three coring sites, a possible positive trend in phosphorus deposition was indicated. The Wakarusa River possibly was a larger contributor of nitrogen and phosphorus to Clinton Lake than was Rock Creek. As a principal limiting factor for primary production in most freshwater environments, phosphorus is of particular importance because increased inputs can contribute to accelerated reservoir eutrophication and the production of algal toxins and taste-and-odor compounds. Trace-element concentrations in the bottom sediment of Clinton Lake generally were uniform over time. As is typical for eastern Kansas reservoirs, arsenic, chromium, and nickel concentrations typically exceeded the threshold-effects guidelines, which represent the concentrations above which toxic biological effects occasionally occur. Zinc concentrations frequently exceeded the threshold-effects guideline. Trace-element concentrations did not exceed the probable-effects guidelines (available for eight trace elements), which represent the concentrations above which toxic biological effects usually or frequently occur. Cyanobacterial akinetes (cyanobacteria resting stage) in the bottom sediment of Clinton Lake, combined with historical water-quality data on chlorophyll-a and total phosphorus concentrations, indicated that the reservoir likely has been eutrophic throughout most of its history. A statistically significant increase in cyanobacterial akinetes in the bottom sediment indicated that Clinton Lake may have become more eutrophic over the life of the reservoir. The increase in cyanobacterial akinetes may, in part, be related to a possible increase in total phosphorus concentrations.

Scientific Investigations Report↗

Trends in suspended-sediment loads and concentrations in the Mississippi River Basin, 1950–2009

Trends in loads and concentrations of suspended sediment and suspended sand generally were downward for stations within the Mississippi River Basin during the 60-, 34-, and 12-year periods analyzed. Sediment transport in the lower Mississippi River has historically been, and continues to be, most closely correlative to sediment contributions from the Missouri River, which generally carried the largest annual suspended-sediment load of the major Mississippi River subbasins. The closure of Fort Randall Dam in the upper Missouri River in 1952 was the single largest event in the recorded historical decline of suspended-sediment loads in the Mississippi River Basin. Impoundments on tributaries and sediment reductions as a result of implementation of agricultural conservation practices throughout the basin likely account for much of the remaining Mississippi River sediment transport decline. Scour of the main-stem channel downstream from the upper Missouri River impoundments is likely the largest source of suspended sand in the lower Missouri River. The Ohio River was second to the Missouri River in terms of sediment contributions, followed by the upper Mississippi and Arkansas Rivers. Declines in sediment loads and concentrations continued through the most recent analysis period (1998–2009) at available Mississippi River Basin stations. Analyses of flow-adjusted concentrations of suspended sediment indicate the recent downward temporal changes generally can be explained by corresponding decreases in streamflows.

Mississippi River Basin↗

Concentration, flux, and the analysis of trends of total and dissolved phosphorus, total nitrogen, and chloride in 18 tributaries to Lake Champlain, Vermont and New York, 1990–2011

Annual concentration, flux, and yield for total phosphorus, dissolved phosphorus, total nitrogen, and chloride for 18 tributaries to Lake Champlain were estimated for 1990 through 2011 using a weighted regression method based on time, tributary streamflows (discharges), and seasonal factors. The weighted regression method generated two series of daily estimates of flux and concentration during the period of record: one based on observed discharges and a second based on a flow-normalization procedure that removes random variation due to year-to-year climate-driven effects. The flownormalized estimate for a given date is similar to an average estimate of concentration or flux that would be made if all of the observed discharges for that date were equally likely to have occurred. The flux bias statistic showed that 68 of the 72 flux regression models were minimally biased. Temporal trends in the concentrations and fluxes were determined by calculating percent changes in flow-normalized annual fluxes for the full period of analysis (1990 through 2010) and for the decades 1990–2000 and 2000–2010. Basinwide, flow-normalized total phosphorus flux decreased by 42 metric tons per year (t/yr) between 1990 and 2010. This net result reflects a basinwide decrease in flux of 21 metric tons (t) between 1990 and 2000, followed by a decrease of 20 t between 2000 and 2010; both results were largely influenced by flux patterns in the large tributaries on the eastern side of the basin. A comparison of results for total phosphorus for the two separate decades of analysis found that more tributaries had decreasing concentrations and flux rates in the second decade than the first. An overall reduction in dissolved phosphorus flux of 0.7 t/yr was seen in the Lake Champlain Basin during the full period of analysis. That very small net change in flux reflects substantial reductions between 1990 and 2000 from eastern tributaries, especially in Otter Creek and the LaPlatte and Winooski Rivers that largely were offset by increases in the Missisquoi and Saranac Rivers in the second decade (between 2000 and 2010). The number of tributaries that had increases in dissolved phosphorus concentrations stayed constant at 13 or 14 during the period of analysis. Total nitrogen concentration and flux for most of the monitored tributaries in the Lake Champlain Basin have decreased since 1990. Between 1990 and 2010, flow-normalized total nitrogen flux decreased by 386 t/yr, which reflects an increase of 440 t/yr between 1990 and 2000 and a decrease of 826 t/yr between 2000 and 2010. All individual tributaries except the Winooski River had decreases in total nitrogen concentration and flux between 2000 and 2010. The decrease in total nitrogen flux over the period of record could be related to the decrease in nitrogen from atmospheric deposition observed in Vermont or to concurrent benefits realized from the implementation of agricultural best-management practices in the Lake Champlain Basin that were designed primarily to reduce phosphorus runoff. For chloride, large increases in flow-normalized concentrations and flux between 1990 and 2000 for 17 of the 18 tributaries diminished to small increases or decreases between 2000 and 2010. Between 1990 and 2010, flow-normalized flux increased by 32,225 t/yr, 78 percent of which (25,163 t) was realized during the first decade, from 1990 through 2000. The five tributaries that had decreasing concentration and flux of chloride between 2000 and 2010 were all on the eastern side of Lake Champlain, possibly related to reductions since 1999 in winter road salt application in Vermont. Positive correlations of phosphorus flux and changes in phosphorus concentration and flux in tributaries with phosphorus inputs to basins from point sources, suggest that point sources have an effect on stream phosphorus chemistry. Several measures of changes in agricultural statistics, such as agricultural land use, acres of land in farms, acres of cropland, and acres of corn for grain or seed, are positively correlated with changes in phosphorus concentration or flux in the tributaries. Negative correlations of the amount of money spent on agricultural best-management practices with changes in phosphorus concentration or flux in the tributaries, suggest that best-management practices may be an effective tool, along with point-source reductions, in making progress towards management goals for phosphorus reductions in Lake Champlain.

New York;Vermont↗

Water withdrawals, use, and trends in Florida, 2010

In 2010, the total amount of water withdrawn in Florida was estimated to be 14,988 million gallons per day (Mgal/d). Saline water accounted for 8,589 Mgal/d (57 percent) and freshwater accounted for 6,399 Mgal/d (43 percent). Groundwater accounted for 4,166 Mgal/d (65 percent) of freshwater withdrawals, and surface water accounted for the remaining 2,233 Mgal/d (35 percent). Surface water accounted for nearly all (99.9 percent) saline-water withdrawals. An additional 659 Mgal/d of reclaimed wastewater was used in Florida during 2010. Freshwater withdrawals were greatest in Palm Beach County (707 Mgal/d), and saline-water withdrawals were greatest in Hillsborough County (1,715 Mgal/d). Fresh groundwater provided drinking water (public supplied and self-supplied) for 17.33 million people (92 percent of Florida’s population), and fresh surface water provided drinking water for 1.47 million people (8 percent). The statewide public-supply gross per capita use for 2010 was 134 gallons per day, whereas the statewide public-supply domestic per capita use was 85 gallons per day. The majority of groundwater withdrawals (almost 62 percent) in 2010 were obtained from the Floridan aquifer system, which is present throughout most of the State. The majority of fresh surface-water withdrawals (56 percent) came from the southern Florida hydrologic unit subregion and is associated with Lake Okeechobee and the canals in the Everglades Agricultural Area of Glades, Hendry, and Palm Beach Counties, as well as the Caloosahatchee River and its tributaries in the agricultural areas of Collier, Glades, Hendry, and Lee Counties. Overall, agricultural irrigation accounted for 40 percent of the total freshwater withdrawals (ground and surface), followed by public supply with 35 percent. Public supply accounted for 48 percent of groundwater withdrawals, followed by agricultural self-supplied (34 percent), commercial-industrial-mining self-supplied (7 percent), recreational-landscape irrigation and domestic self-supplied (5 percent each), and power generation (less than 1 percent). Agricultural self-supplied accounted for 51 percent of fresh surface-water withdrawals, followed by power generation (25 percent), public supply (11 percent), recreational-landscape irrigation (9 percent), and commercial-industrial-mining self-supplied (4 percent). Power generation accounted for nearly all (99.8 percent) saline-water withdrawals. Of the 18.80 million people who resided in Florida during 2010, 41 percent (7.68 million people) resided in the South Florida Water Management District (SFWMD), 25 percent each resided in the Southwest Florida Water Management District (SWFWMD) and the St. Johns River Water Management District (SJRWMD) (4.73 and 4.70 million people, respectively), 7 percent (1.36 million people) resided in the Northwest Florida Water Management District (NWFWMD), and 2 percent (0.33 million people) resided in the Suwannee River Water Management District (SRWMD). The largest percentage of freshwater withdrawals was from the SFWMD (47 percent), followed by the SJRWMD (21 percent), SWFWMD (18 percent), NWFWMD (9 percent), and SRWMD (5 percent). Between 1950 and 2010, the population of Florida increased by 16.03 million (580 percent), and the total water withdrawals (fresh and saline) increased by 12,334 Mgal/d (465 percent). More recently, total freshwater withdrawals decreased by more than 1,792 Mgal/d (22 percent) between 2000 and 2010, while the population increased by 2.82 million (18 percent), and total freshwater withdrawals decreased by more than 474 Mgal/d (7 percent) between 2005 and 2010, while the population increased by 0.88 million (8 percent). The recent trend of decreases in freshwater withdrawals is a result of increased rainfall during this period, the development and use of alternative water sources, water conservation efforts, more conservative regulations and mandates, changes in economic conditions, and losses of irrigated lands. Fresh-water withdrawals for public supply, agricultural self-supplied use, and commercial-industrial-mining self-supplied use all decreased between 2000 and 2010 and between 2005 and 2010, whereas freshwater withdrawals for domestic self-supplied use, recreational-landscape irrigation use, and power generation use either remained the same or changed slightly during the decade. The use of highly mineralized groundwater (referred to as nonpotable water) as a source of drinking water has increased in Florida. Nonpotable water use for public supply has increased from nearly 2 Mgal/d in 1970 to about 165 Mgal/d in 2010. Nonpotable water is either blended or treated to meet drinking-water standards and is mostly used along the east and west coasts of central and southern Florida. The use of reclaimed wastewater increased from about 206 Mgal/d in 1986 to nearly 659 Mgal/d in 2010. More than three-quarters (79 percent) of reclaimed wastewater in 2010 was used to supplement potable-quality water withdrawals for urban irrigation, agricultural irrigation, and industrial use.

Florida↗

Potentiometric surfaces and water-level trends in the Cockfield (upper Claiborne) aquifer in southern Arkansas and the Wilcox (lower Wilcox) aquifer of northeastern and southern Arkansas, 2012

The Cockfield aquifer, located in southern Arkansas, is composed of Eocene-age sand beds found near the base of the Cockfield Formation of Claiborne Group. The Wilcox aquifer, located in northeastern and southern Arkansas, is composed of Paleocene-age sand beds found in the middle to lower part of the Wilcox Group. The Cockfield and Wilcox aquifers are primary sources of groundwater. In 2010, withdrawals from the Cockfield aquifer in Arkansas totaled 19.2 million gallons per day (Mgal/d), and withdrawals from the Wilcox aquifer totaled 36.5 Mgal/d. A study was conducted by the U.S. Geological Survey in cooperation with the Arkansas Natural Resources Commission and the Arkansas Geological Survey to measure water levels associated with the Cockfield aquifer and the Wilcox aquifer in northeastern and southern Arkansas. Water levels were measured at 43 wells completed in the Cockfield aquifer and 47 wells completed in the Wilcox aquifer in February and March 2012. Measurements from 2012 are presented as potentiometric-surface maps and in combination with measurements from 2006 as water-level difference maps. Trends in water-level change over time within the Cockfield and Wilcox aquifers were determined using the water-level difference maps and selected well hydrographs. The Cockfield aquifer study area in southern Arkansas is bounded on the east by the Mississippi River and on the west by the area that contains outcrops and subcrops of the Cockfield Formation. The northern boundary of the Cockfield aquifer study area is defined by the area that contains observation wells completed in the Cockfield aquifer and the southern boundary is the Louisiana State line. The Wilcox aquifer study area in northeastern Arkansas is bounded on the east by the Mississippi River and on the north by the Missouri State line. The southern and western boundaries are defined by areas containing observation wells completed in the Wilcox aquifer or by outcrop areas on or near Crowleys Ridge. The Wilcox aquifer study area in southern Arkansas is defined by observation wells completed in the Wilcox aquifer or by areas that contain outcrops of the Wilcox Group, or both. The potentiometric-surface map of the Cockfield aquifer shows the regional direction of groundwater flow was generally toward the east-southeast, except in areas of intense groundwater withdrawals such as southwestern Ashley County, where groundwater flows toward the town of Crossett. The highest water-level altitude measured was 350 feet (ft) above National Geodetic Vertical Datum of 1929 (NGVD 29) in central Columbia County. The lowest water-level altitude measured was 40 ft above NGVD 29 in southeastern Lincoln County. The water-level difference map for the Cockfield aquifer in Arkansas was constructed using 42 water-level measurements made during 2006 and 2012. The difference in water levels for the Cockfield aquifer ranged from 27.4 ft to -10.4 ft. The largest water-level rise was in Calhoun County, and the largest water-level decline was 10.4 ft in Union County. Of the 42 wells, 13 wells had a rise in water level, and the remaining 29 wells had a decline in water level. Hydrographs for 32 wells in the Cockfield aquifer with historical water-level data were evaluated using linear regression to calculate the annual rise or decline for each well. These data were aggregated by county and statistically evaluated for the range, mean, and median of water-level change in each county. Hydrographs for Bradley, Calhoun, Chicot, Columbia, and Union Counties indicated both rising and declining water levels. The mean annual water-level rise or decline for Calhoun County was 0.00 foot per year (ft/yr) or unchanged. The mean annual water-level for Ashley, Bradley, Chicot, Cleveland, Columbia, Lincoln, and Union Counties show declines ranging from -0.02 to -1.10 ft/yr. Two potentiometric-surface maps, one for the southern area and one for the northeastern area, were constructed to show the altitude of the water surface in the Wilcox aquifer. The direction of groundwater flow in the northeastern area was generally towards the south-southwest except for some areas immediately adjacent to the Mississippi River where the flow was more eastward towards the river. The highest water-level altitude was 219 ft in northern Mississippi County, and the lowest water-level altitude was 123 ft near West Memphis in Crittenden County. The direction of groundwater flow in the northern part of the southern area was generally towards the southwest. The direction of groundwater flow in the southern part was in all directions because of two cones of depression and two water-level mounds. The highest water-level altitude measured was 394 ft at the center of a water-level mound in eastern Hot Spring County and a water-level mound in southwestern Hempstead County. The lowest water-level altitude measured was 145 ft at the center of the cone of depression in Clark County. Water-level difference maps for the Wilcox aquifer in Arkansas were constructed using 47 water-level measurements made during 2006 and 2012. The difference in water levels for the Wilcox aquifer in the northeastern area ranged from 22.0 ft to -17.9 ft. The largest rise in water level occurred in Crittenden County, and the largest decline occurred in Lee County. Twenty-one wells had rising water levels, and 10 wells had declining water levels. The difference in water levels for the Wilcox aquifer in the southern area ranged from 18.1 ft to -4.2 ft. The largest rise and the largest decline in water level occurred in Nevada County. Twelve wells had rising water levels, and 4 wells had declining water levels. Linear regression analysis of long-term hydrographs was used to determine the mean annual water-level rise and decline in the Wilcox aquifer in the northeastern and southern areas of Arkansas. In the northeastern area, the mean annual water level declined in all seven counties. The mean annual declines ranged from -0.55 ft/yr in Craighead County to -1.46 ft/yr in St. Francis County. In the southern area, the annual rise and decline calculations for wells with over 20 years of records indicate rising and declining water levels in Clark, Hot Spring, and Nevada Counties. The mean annual water level declined in all counties except Hot Spring County.

Arkansas↗

Water-level trends and potentiometric surfaces in the Nacatoch Aquifer in northeastern and southwestern Arkansas and in the Tokio Aquifer in southwestern Arkansas, 2014–15

The Nacatoch Sand in northeastern and southwestern Arkansas and the Tokio Formation in southwestern Arkansas are sources of groundwater for agricultural, domestic, industrial, and public use. Water-level altitudes measured in 51 wells completed in the Nacatoch Sand and 42 wells completed in the Tokio Formation during 2014 and 2015 were used to create potentiometric-surface maps of the two areas. Aquifers in the Nacatoch Sand and Tokio Formation are hereafter referred to as the Nacatoch aquifer and the Tokio aquifer, respectively. Potentiometric surfaces show that groundwater in the Nacatoch aquifer flows southeast toward the Mississippi River in northeastern Arkansas. Groundwater flow direction is towards the south and southeast in Hempstead, Little River, and Nevada Counties in southwestern Arkansas. An apparent cone of depression exists in southern Clark County and likely alters groundwater flow from a regional direction toward the depression. In southwestern Arkansas, potentiometric surfaces indicate that groundwater flow in the Tokio aquifer is towards the city of Hope. Northwest of Hope, an apparent cone of depression exists. In southwestern Pike, northwestern Nevada, and northeastern Hempstead Counties, an area of artesian flow (water levels are at or above land surface) exists. Water-level changes in wells were identified using two methods: (1) linear regression analysis of hydrographs from select wells with a minimum of 20 years of water-level data, and (2) a direct comparison between water-level measurements from 2008 and 2014–15 at each well. Of the six hydrographs analyzed in the Nacatoch aquifer, four indicated a decline in water levels. Compared to 2008 measurements, the largest rise in water levels was 35.14 feet (ft) in a well in Clark County, whereas the largest decline was 14.76 ft in a well in Nevada County, both located in southwestern Arkansas. Of the four hydrographs analyzed in the Tokio aquifer, one indicated a decline in water levels, while the others remained relatively unchanged. Compared to 2008 measurements, the largest rise in water levels was 21.34 ft in Hempstead County, and the largest water-level decline was 39.37 ft in Clark County. Although changes in water levels since 2008 are spatially varied; long-term trends indicate an overall decline in water levels in both aquifers.

Arkansas↗

Assessment of conservation management practices on water quality and observed trends in the Plum Creek Basin, 2010–20

The U.S. Geological Survey and University of Wisconsin–Green Bay collected hydrologic and water-quality data to assess the effectiveness of agricultural conservation management practice (CMP) implementation at mainstem Plum Creek and west Plum Creek in northeastern Wisconsin. These two subbasins cover 88 percent of the Plum Creek Basin (Hydrologic Unit Code 12), which is a subbasin of the lower Fox River Basin. A published total maximum daily load report for the lower Fox River Basin rated Plum Creek as one of the greatest contributors of total suspended solids (TSS) and total phosphorus (TP) draining into the lower Fox River. To reduce TSS and TP exports from Plum Creek, additional cropland conservation practices and watercourse protections were applied between 2012 and 2020. To detect water-quality trends, data were collected during 2010 to 2020 at mainstem Plum Creek and 2013 to 2020 at west Plum Creek. The project used two methods to evaluate CMP effectiveness. The first method focused on evaluating water-quality changes between initial and post-CMP implementation periods during rain- or snowmelt-induced runoff events (hereafter referred to as “events”). In this approach random-forest models were developed to account for environmental factors which influence water quality. Model residuals from the two time periods were compared to determine the significance of water-quality changes associated with CMP implementation for mainstem and west Plum Creek Basins. The second method used a Weighted Regressions on Time, Discharge, and Season time-series approach to examine changes in water quality during the entire study period in mainstem Plum Creek. Results from both methods indicated there were minimal water-quality changes in TSS concentrations and flow-normalized delivery during runoff events during the 10-year period from 2010 to 2020; however, TP concentrations during low streamflow (less than 3 cubic feet per second [ft 3 /s]) may have decreased. The lack of observed improvement may be attributable to any of the following: variability in weather and hydrologic conditions, insufficient post-treatment data, additional cropland being converted to corn production, above average rainfall, streambank degradation, acute and legacy sources of phosphorus from farm fields, excessive/vulnerable manure applications and spills, and point-source discharges.

Wisconsin↗

Streamflow characteristics and trends in New Jersey, water years 1903–2017

As New Jersey’s population density remains high, so does its requirements for water management. Understanding the streamflow conditions throughout the state and how they may have changed over time is an important part of managing the water resources within the state. The New Jersey Department of Environmental Protection has many responsibilities related to protecting the environment and natural resources and among them is protecting the waters in the lakes, rivers, and streams of New Jersey for current and future use. To support this mission, the U.S. Geological Survey updated high- and low-streamflow statistics for 97 continuous-record streamgages and low-streamflow statistics for 719 partial-record streamgages throughout the state. The continuous-record streamgages included in the study had a minimum of 20 years of record, spanning from 1903 to 2017. This study is an update to previous studies that documented the high- and low-streamflow statistics for New Jersey streams in the 1970s and in 2005. The 1982 report by Gillespie and Schopp documented low-flow characteristics and flow duration for about 400 continuous and partial-record streamgages. The U.S. Geological Survey computed streamflow statistics including, but not limited to, maximum, minimum, and means for period of record, flow durations, nonexceedance high- and low-flow frequencies, base flow, runoff, peak-to-mean flow ratios, and September median streamflow. Overall, both high and low flows are generally increasing in New Jersey, though the results are not uniform across the State. Streamflow trends and changes to duration and frequency statistics can be influenced by local water use, in addition to climate variables. The resulting computations at some streamgages indicated considerable positive change while others showed considerable negative change. Water managers and regulators can use the data provided here and in the companion data release to assess individual stream reaches and watershed management areas to evaluate the available resources and changes, which may have developed during the periods for which streamflow statistics are available.

New Jersey↗

Decadal trends in the quality of groundwater used for public drinking-water supply in California, 2004–23, California Groundwater Ambient Monitoring and Assessment Program, Priority Basin Project

This study provides a comprehensive assessment of decadal changes in the quality of groundwater used for public drinking-water supply at 444 monitoring sites across California during 2004–23. We assessed decadal step trends in groundwater quality for 145 water-quality constituents and geochemical indicators statewide and across geographic and land-use based network groups. We evaluated the statistical significance of directional changes (predominant increase or decrease of constituent concentrations) and the magnitude of those changes across all network groups. Uranium showed the most widespread directional and high-magnitude increases of all constituents with regulatory benchmarks statewide, particularly in the agriculture-dominated Central Valley as well as urban-and desert-dominated regions of Southern California. Fluoride and perchlorate showed the most widespread directional and high-magnitude decreases of all constituents with regulatory benchmarks statewide, which were also most pronounced in Southern California. Although arsenic and nitrate did not often register significant directional changes across network groups, they showed widespread, high-magnitude changes in both directions (increase and decrease) at levels often exceeding 10 percent of respective regulatory benchmarks statewide. Triazine herbicides (atrazine and simazine) and the gasoline oxygenate methyl tert -butyl ether showed significant directional decreases statewide, but not at levels considered to be of high magnitude compared to respective regulatory benchmarks. We observed significant directional and high-magnitude increases of total dissolved solids statewide, which were most pronounced in agricultural areas. Analysis of explanatory geochemical indicators indicated that prevalent statewide increases of alkalinity and calcium were the predominant components of the observed statewide increases in total dissolved solids by mass. Widespread increases in groundwater alkalinity and calcium across agricultural and urban areas may be related, in part, to warm-season irrigation and other anthropogenic factors that have shifted soil weathering dynamics over the long term. Increasing alkalinity concentrations were related to increasing uranium concentrations, particularly in areas with aquifer materials derived from granitic rocks. Conversely, increasing calcium concentrations were related to decreasing fluoride concentrations, particularly in areas where fluoride occurred naturally at elevated concentrations. Decrease of perchlorate, triazine herbicides, and methyl tert -butyl ether are likely related to decreased anthropogenic source inputs over time and natural attenuation in aquifers.

California↗

Status and trends in suspended-sediment discharges, soil erosion, and conservation tillage in the Maumee River basin: Ohio, Michigan, and Indiana

The relation of suspended-sediment discharges to conservation-tillage practices and soil loss were analyzed for the Maumee River Basin in Ohio, Michigan, and Indiana as part of the U.S. Geological Survey’s National Water-Quality Assessment Program. Cropland in the basin is the largest contributor to soil erosion and suspended-sediment discharge to the Maumee River and the river is the largest source of suspended sediments to Lake Erie. Retrospective and recently-collected data from 1970–98 were used to demonstrate that increases in conservation tillage and decreases in soil loss can be related to decreases in suspended-sediment discharge from streams. Average annual water and suspended-sediment budgets computed for the Maumee River Basin and its principal tributaries indicate that soil drainage and runoff potential, stream slope, and agricultural land use are the major human and natural factors related to suspended-sediment discharge. The Tiffin and St. Joseph Rivers drain areas of moderately to somewhat poorly drained soils with moderate runoff potential. Expressed as a percentage of the total for the Maumee River Basin, the St. Joseph and Tiffin Rivers represent 29.0 percent of the basin area, 30.7 percent of the average-annual streamflow, and 9.31 percent of the average annual suspended-sediment discharge. The Auglaize and St. Marys Rivers drain areas of poorly to very poorly drained soils with high runoff potential. Expressed as a percentage of the total for the Maumee River Basin, the Auglaize and St. Marys Rivers represent 48.7 percent of the total basin area, 53.5 percent of the average annual streamflow, and 46.5 percent of the average annual suspended-sediment discharge. Areas of poorly drained soils with high runoff potential appear to be the major source areas of suspended sediment discharge in the Maumee River Basin. Although conservation tillage differed in the degree of use throughout the basin, on average, it was used on 55.4 percent of all crop fields in the Maumee River Basin from 1993–98. Conservation tillage was used at relatively higher rates in areas draining to the lower main stem from Defiance to Waterville, Ohio and at relatively lower rates in the St. Marys and Auglaize River Basins, and in areas draining to the main stem between New Haven, Ind. and Defiance, Ohio. The areas that were identified as the most important sediment-source areas in the basin were characterized by some of the lowest rates of conservation tillage. The increased use of conservation tillage was found to correspond to decreases in suspended- sediment discharge over time at two locations in the Maumee River Basin. A 49.8 percent decrease in suspended-sediment discharge was detected when data from 1970–74 were compared to data from 1996–98 for the Auglaize River near Ft. Jennings, Ohio. A decrease in suspended-sediment discharge of 11.2 percent was detected from 1970–98 for the Maumee River at Waterville, Ohio. No trends in streamflow at either site were detected over the period 1970–98. The lower rate of decline in suspended-sediment discharge for the Maumee River at Waterville, Ohio compared to the Auglaize River near Ft. Jennings, may be due to resuspension and export of stored sediments from drainage ditches, stream channels, and flood plains in the large drainage basin upstream from Waterville. Similar findings by other investigators about the capacity of drainage networks to store sediment are supported by this investigation. These findings go undetected when soil loss estimates are used alone to evaluate the effectiveness of conservation tillage. Water-quality data in combination with soil-loss estimates were needed to draw these conclusions. These findings provide information to farmers and soil conservation agents about the ability of conservation tillage to reduce soil erosion and suspended-sediment discharge from the Maumee River Basin.

Indiana, Michigan, Ohio↗

Historical trends and concentrations of fecal coliform bacteria in the Brandywine Creek basin, Chester County, Pennsylvania

The Brandywine Creek in Chester County is used for recreation and as an important source of drinking water. For this study, 40 sites were established for collection of water samples for analysis of fecal coliform and Escherichia coli bacteria in 1998-99. Samples were collected during base-flow conditions and during five storms in which rainfall exceeded 0.5 inch. During base- flow conditions, the median concentrations of fecal coliform bacteria exceeded 200 col/100 mL at 26 of the 40 sites (65 percent). During stormflow conditions, the median concentration of fecal coliform bacteria exceeded the Pennsylvania Department of Environmental Protection (PaDEP) criterion of 200 col/100 mL at 30 of 33 sites sampled (91 percent). Trends in fecal coliform bacteria concentrations were downward for the period 1973-99 at three long-term water-quality monitor stations, the result of upgrades in wastewater treatment plants, decreases in point-source discharges, and a decrease in agricultural land. A positive relation exists between streamflow and concentrations of fecal coliform bacteria at two of the long-term stations, but concentrations are elevated in base flow and stormflow at all three stations. Factors affecting bacteria concentrations in the Brandywine Creek Basin include nonpoint-source contaminants, reservoirs, seasonality, and stormflow. Nonpoint sources of bacterial contamination in the basin include, but are not limited to, land-surface runoff, urbanization, agricultural processes, groundwater contamination, and wildlife. Bacteria concentrations in streams that flow directly from the reservoirs are much lower than the concentrations in the streams flowing into the reservoirs. During March, April, May, October, and November, the Brandywine Creek tends to have lower water temperatures and bacteria concentrations than during June, July, August, and September. The 10-year median concentrations of bacteria at West Branch Brandywine Creek at Modena and East Branch Brandywine Creek below Downingtown exceed the criterion of 200 col/100 mL established by the PaDEP during the swimming season. The 10-year median concentrations of bacteria at Brandywine Creek at Chadds Ford exceed the criterion of 200 col/100 mL only during June. None of the stations exceed the criterion of 2,000 col/100 mL as established by the PaDEP for the remainder of the year.

Water-Resources Investigations Report↗