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Results for “Parasitology”

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At least 91 records · Page 5Linked to original sources
USGS2026

Effects of fipronil bait pellets on two cricetid species: Potential implications for plague mitigation and wildlife conservation

We evaluated the effects of fipronil bait pellets on two cricetids that commonly occupy colonies of black-tailed prairie dogs ( Cynomys ludovicianus ; BTPDs): western deer mice ( Peromyscus sonoriensis ) and northern grasshopper mice ( Onychomys leucogaster ). In one experiment, bait pellets (0.96 mg fipronil/bait) were applied at 75 baits/ha to three 1.44-ha plots on a BTPD colony. Mouse abundance declined by 70% from before to 6-10 d after treatment. In a second experiment, bait pellets (0.46 or 1.52 mg fipronil/bait) were applied at 125 baits/ha to four plots (0.85-1.86 ha) on two BTPD colonies; two non-treated plots were baselines (1.09 and 2.06 ha). From before to 11-15 d after treatment, mouse abundance declined by 51%- 67% on the treated plots vs. a decline of 9% on the non-treated plots. Mouse survival from before to 11-15 d after treatment was 51% lower on the treated plots. In a third experiment, bait pellets (0.84 mg fipronil/bait) were applied at 125 baits/acre on two 1.44-ha plots on a BTPD colony; two 1.44-ha non-treated plots were baselines. Mouse survival from before to 30-44 d after treatment was 45% lower on the treated plots; the abundance of deer mice on the treated plots remained similar from before to 30-44 d after treatment, perhaps due to juvenile recruitment and/or immigration. In a laboratory experiment, 33 deer mice offered one bait pellet (0.86 mg fipronil/bait) consumed 27% of their bait, on average (range = 0-100%). Over 3 d, deer mouse mortality was estimated at 53%; mortality increased with fipronil dose, which averaged 11 mg fipronil/kg body mass (range = 3-46 mg/kg). Brain samples were available from 31 deer mice; all tested positive for fipronil sulfone, the primary mammalian metabolite of fipronil, at 19 to 61,205 ng fipronil sulfone/g. Additional experiments could determine if these findings scale up to larger landscapes.

South Dakota↗
USGS2009

Parasitic castration: the evolution and ecology of body snatchers

Castration is a response to the tradeoff between consumption and longevity faced by parasites. Common parasitic castrators include larval trematodes in snails, and isopod and barnacle parasites of crustaceans. The infected host (with its many unique properties) is the extended phenotype of the parasitic castrator. Because an individual parasitic castrator can usurp all the reproductive energy from a host, and that energy is limited, intra- and interspecific competition among castrators is generally intense. These parasites can be abundant and can substantially depress host density. Host populations subject to high rates of parasitic castration appear to respond by maturing more rapidly.

Trends in Parasitology↗
USGS2014

Sapronosis: a distinctive type of infectious agent

Sapronotic disease agents have evolutionary and epidemiological properties unlike other infectious organisms. Their essential saprophagic existence prevents coevolution, and no host–parasite virulence trade-off can evolve. However, the host may evolve defenses. Models of pathogens show that sapronoses, lacking a threshold of transmission, cannot regulate host populations, although they can reduce host abundance and even extirpate their hosts. Immunocompromised hosts are relatively susceptible to sapronoses. Some particularly important sapronoses, such as cholera and anthrax, can sustain an epidemic in a host population. However, these microbes ultimately persist as saprophages. One-third of human infectious disease agents are sapronotic, including nearly all fungal diseases. Recognition that an infectious disease is sapronotic illuminates a need for effective environmental control strategies.

Trends in Parasitology↗
USGS2015

How do humans affect wildlife nematodes?

Human actions can affect wildlife and their nematode parasites. Species introductions and human-facilitated range expansions can create new host–parasite interactions. Novel hosts can introduce parasites and have the potential to both amplify and dilute nematode transmission. Furthermore, humans can alter existing nematode dynamics by changing host densities and the abiotic conditions that affect larval parasite survival. Human impacts on wildlife might impair parasites by reducing the abundance of their hosts; however, domestic animal production and complex life cycles can maintain transmission even when wildlife becomes rare. Although wildlife nematodes have many possible responses to human actions, understanding host and parasite natural history, and the mechanisms behind the changing disease dynamics might improve disease control in the few cases where nematode parasitism impacts wildlife.

Trends in Parasitology↗
USGS2018

To reduce the global burden of human schistosomiasis, use ‘old fashioned’ snail control

Control strategies to reduce human schistosomiasis have evolved from ‘snail picking’ campaigns, a century ago, to modern wide-scale human treatment campaigns, or preventive chemotherapy. Unfortunately, despite the rise in preventive chemotherapy campaigns, just as many people suffer from schistosomiasis today as they did 50 years ago. Snail control can complement preventive chemotherapy by reducing the risk of transmission from snails to humans. Here, we present ideas for modernizing and scaling up snail control, including spatiotemporal targeting, environmental diagnostics, better molluscicides, new technologies (e.g., gene drive), and ‘outside the box’ strategies such as natural enemies, traps, and repellants. We conclude that, to achieve the World Health Assembly’s stated goal to eliminate schistosomiasis, it is time to give snail control another look.

Trends in Parasitology↗
USGS2020

Modeling pathogen dispersal in marine fish and shellfish

Bio-physical models are a useful tool for understanding dispersal and transmission of marine pathogens. While utilized for larval dispersal models, they are only recently being used in epidemiological studies and are currently underutilized by the marine epidemiology field. Bio-physical models are useful for spatial planning and coastal management. For example, they have been used for spatial planning of salmon farm site locations, and to establish early warning networks. Bio-physical modeling can be used to test hypotheses, rather than simply develop them. Model resolution and computation demands must be balanced when making decisions about model parameters. Epidemiological bio-physical models are in their infancy. While they have proven useful so far, future applications of these models can incorporate more aspects of disease dynamics and address many additional questions.

Trends in Parasitology↗
USGS2007

Effects of supplemental feeding on gastrointestinal parasite infection in Rocky Mountain Elk (Cervus elaphus): Preliminary observations

The effects of management practices on the spread and impact of parasites and infectious diseases in wildlife and domestic animals are of increasing concern worldwide, particularly in cases where management of wild species can influence disease spill-over into domestic animals. In the Greater Yellowstone Ecosystem, USA, winter supplemental feeding of Rocky Mountain elk ( Cervus elaphus ) may enhance parasite and disease transmission by aggregating elk on feedgrounds. In this study, we tested the effect of supplemental feeding on gastrointestinal parasite infection in elk by comparing fecal egg/oocyst counts of fed and unfed elk. We collected fecal samples from fed and unfed elk at feedground and control sites from January to April 2006, and screened all samples for parasites. Six different parasite types were identified, and 48.7% of samples were infected with at least one parasite. Gastrointenstinal (GI) nematodes (Nematoda: Strongylida), Trichuris spp., and coccidia were the most common parasites observed. For all three of these parasites, fecal egg/oocyst counts increased from January to April. Supplementally fed elk had significantly higher GI nematode egg counts than unfed elk in January and February, but significantly lower counts in April. These patterns suggest that supplemental feeding may both increase exposure and decrease susceptibility of elk to GI nematodes, resulting in differences in temporal patterns of egg shedding between fed and unfed elk.

Wyoming↗
USGS2008

Genetic diversity among sea otter isolates of Toxoplasma gondii

Sea otters ( Enhydra lutris ) have been reported to become infected with Toxoplasma gondii and at times succumb to clinical disease. Here, we determined genotypes of 39 T. gondii isolates from 37 sea otters in two geographically distant locations (25 from California and 12 from Washington). Six genotypes were identified using 10 PCR-RFLP genetic markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico, and by DNA sequencing of loci SAG1 and GRA6 in 13 isolates. Of these 39 isolates, 13 (33%) were clonal Type II which can be further divided into two groups at the locus Apico. Two of the 39 isolates had Type II alleles at all loci except a Type I allele at locus L358. One isolate had Type II alleles at all loci except the Type I alleles at loci L358 and Apico. One isolate had Type III alleles at all loci except Type II alleles at SAG2 and Apico. Two sea otter isolates had a mixed infection. Twenty-one (54%) isolates had an unique allele at SAG1 locus. Further genotyping or DNA sequence analysis for 18 of these 21 isolates at loci SAG1 and GRA6 revealed that there were two different genotypes, including the previously identified Type X (four isolates) and a new genotype named Type A (14 isolates). The results from this study suggest that the sea otter isolates are genetically diverse.

California, Washington↗
USGS2011

Sarcocystis neurona retinochoroiditis in a sea otter ( Enhydra lutris kenyoni )

Sarcocystis neurona is an important cause of fatal disease in sea otters in the USA. Encephalitis is the predominant lesion and parasites are confined to the central nervous system and muscles. Here we report retinochoroiditis in a sea otter ( Enhydra lutris kenyoni ) found dead on Copalis Beach, WA, USA. Salient lesions were confined to the brain and eye. Multifocal nonsuppurative meningoencephalitis was present in the cerebrum and cerebellum associated with S. neurona schizonts. The retina of one eye had a focus of inflammation that contained numerous S. neurona schizonts and merozoites. The focus extended from the retinal pigment epithelium inward through all layers of the retina, but inflammation was most concentrated at the inner surface of the tapetum and the outer retina. The inner and outer nuclear layers of the retina were disorganized and irregular at the site of inflammation. There was severe congestion and mild hemorrhage in the choroid, and mild hemorrhage into the vitreous body. Immunohistochemistry with S. neurona -specific polyclonal rabbit antibodies stained schizonts and merozoites. To our knowledge this is the first report of S. neurona -associated retinochoroiditis in any naturally infected animal.

Washington↗
USGS2018

An update on Toxoplasma gondii infections in northern sea otters (Enhydra lutris kenyoni) from Washington State, USA

Toxoplasmosis in marine mammals is epidemiologically and clinically important. Toxoplasma gondii antibodies (by modified agglutination test, cut-off ≥1:25) were detected in serum of 65 of 70 (92.9%) northern sea otters ( Enhydra lutris kenyoni ) from Washington State, USA. Brains and/or muscles of 44 sea otters were bioassayed in mice (INF-γ knock-out [KO], Swiss Webster outbred [SW]) and viable T. gondii was isolated from 22 of 44 (50%); T. gondii strains were lethal to KO mice but not SW mice. These T. gondii isolates were further propagated in cell culture. Multi-locus PCR-RFLP genotyping of cell culture-derived tachyzoites revealed four different genotypes among 22 isolates including ToxoDB PCR-RFLP genotype #5 (14 isolates), #1 (three isolates), #3 (four isolates), and #167 (one isolate). PCR-DNA sequencing based genotyping using polymorphic gene GRA6 revealed one of four different alleles. Among the 14 RFLP genotype #5 strains, 10 have GRA6 sequences that match with the Type A, one match with the Type X, two strains did not generate sequence data, and one strain had double peaks at known polymorphic sites indicating a mixed infection. The seven strains belong to genotypes #1 and #3, all have identical sequences to T. gondii Type II reference isolate ME49. Genotype #167 strain has identical sequence to Type I reference strain. In summary, we observed high seroprevalence, and high rate of isolation of T. gondii from northern sea otters and predominant genotype #5 that has been previously reported a dominant and widespread strain among terrestrial wildlife in North America. GRA6 sequence analysis of the genotype #5 isolates indicated the dominance of Type A lineage in sea otters in Washington State.

Washington↗
USGS2000

Biological characterisation of Sarcocystis neurona isolated from a Southern sea otter (Enhydra lutris nereis)

Sarcocystis neurona was isolated from the brain of a juvenile, male southern sea otter ( Enhydra lutris nereis ) suffering from CNS disease. Schizonts and merozoites in tissue sections of the otter’s brain reacted with anti- S. neurona antiserum immunohistochemically. Development in cell culture was by endopolyogeny and mature schizonts were first observed at 3 days postinoculation. PCR of merozoite DNA using primer pairs JNB33/JNB54 and restriction enzyme digestion of the 1100 bp product with Dra I indicated the organism was S. neurona . Four of four interferon-γ gene knockout mice inoculated with merozoites developed S. neurona -associated encephalitis. Antibodies to S. neurona but not Sarcocystis falcatula , Toxoplasma gondii , or Neospora caninum were present in the serum of inoculated mice. This is the first isolation of S. neurona from the brain of a non-equine host.

International Journal for Parasitology↗
USGS2003

Toxoplasma gondii, Neospora caninum, Sarcocystis neurona, and Sarcocystis canis-like infections in marine mammals

Toxoplasma gondii, Neospora caninum, Sarcocystis neurona, and S. canis are related protozoans that can cause mortality in many species of domestic and wild animals. Recently, T. gondii and S. neurona were recognized to cause encephalitis in marine mammals. As yet, there is no report of natural exposure of N. caninum in marine mammals. In the present study, antibodies to T. gondii and N. caninum were assayed in sera of several species of marine mammals. For T. gondii, sera were diluted 1:25, 1:50, and 1:500 and assayed in the T. gondii modified agglutination test (MAT). Antibodies (MAT a?Y1:25) to T. gondii were found in 89 of 115 (77%) dead, and 18 of 30 (60%) apparently healthy sea otters (Enhydra lutris), 51 of 311 (16%) Pacific harbor seals (Phoca vitulina), 19 of 45 (42%) sea lions (Zalophus californianus), 5 of 32 (16%) ringed seals (Phoca hispida), 4 of 8 (50%) bearded seals (Erignathus barbatus), 1 of 9 (11.1%) spotted seals (Phoca largha), 138 of 141 (98%) Atlantic bottlenose dolphins (Tursiops truncatus), and 3 of 53 (6%) walruses (Odobenus rosmarus). For N. caninum, sera were diluted 1:40, 1:80, 1:160, and 1:320 and examined with the Neospora agglutination test (NAT) using mouse-derived tachyzoites. NAT antibodies were found in 3 of 53 (6%) walruses, 28 of 145 (19%) sea otters, 11 of 311 (3.5%) harbor seals, 1 of 27 (3.7%) sea lions, 4 of 32 (12.5%) ringed seals, 1 of 8 (12.5%) bearded seals, and 43 of 47 (91%) bottlenose dolphins. To our knowledge, this is the first report of N. caninum antibodies in any marine mammal, and the first report of T. gondii antibodies in walruses and in ringed, bearded, spotted, and ribbon seals. Current information on T. gondii-like and Sarcocystis-like infections in marine mammals is reviewed. New cases of clinical S. canis and T. gondii infections are also reported in sea lions, and T. gondii infection in an Antillean manatee (Trichechus manatus manatus).

Alaska, California, Washington↗
USGS2009

A fungus disease in fishes of the Gulf of Maine

1. A fungus disease of epidemic proportions was found in the common sea herring (Clupea harengus) throughout the Gulf of Maine. 2. The fungus was also found to infect the common winter flounder (Pseudopleuronectes americanus) and the alewife (Pomobolus pseudoharengus). 3. The causative agent was found to be a species of fungus belonging to the genus Ichthyosporidium Caullery and Mesnil (1905). The specific name is tentatively accepted as hoferi Plehn and Muslow (1911). 4. The organism is believed to be a normal parasite to the herring and reaches epidemic proportions only when certain unknown factors are operative. 5. The causative organism was found in herring preserved in 1926, and it is believed that the epidemic has been increasing in severity since that time. 6. It is believed that such an epidermic,once initiated, increases in severity, reaches a peak, and subsides to a subpatent level. The peak is believed to have been reached in 1931. 7. The life history and effects of the organism in the herring and flounder are described. 8. The herring is believed to acquire the infection by the ingestion of parasites liberated from fish in the same school. 9. The flounder is believed to acquire the infection by the consumption of infected herring. 10. The alewife is believed to acquire the infection by ingestion of the parasite during its infrequent association with the herring. 11. Infection is believed to be established by way of the alimentary canal and, once established, to spread throughout the host by way of the blood stream or the lymphatics. 12. Direct cross infection from the herring to the flounder establishes the theory that the parasites in these two hosts are one and the same organism. 13. Direct cross infection experiments from the herring to the flounder eliminate the necessity of an intermediate host. 14. There is no reason to believe that this parasite is capable of infecting warm-blooded animals.

Maine, New Hampshire, Massachusetts, New Brunswick↗
USGS2009

Wildlife disease and conservation in Hawaii: pathogenicity of avian malaria (Plasmodium relictum) in experimentally infected Iiwi (Vestiaria coccinea)

Native Hawaiian forest birds are facing a major extinction crisis with more than 75% of species recorded in historical times either extinct or endangered. Reasons for this catastrophe include habitat destruction, competition with non-native species, and introduction of predators and avian diseases. We tested susceptibility of Iiwi (Vestiaria coccinea), a declining native species, and Nutmeg Mannikins (Lonchura punctulata), a common non-native species, to an isolate of Plasmodium relictum from the island of Hawaii. Food consumption, weight, and parasitaemia were monitored in juvenile Iiwi that were infected by either single (low-dose) or multiple (high-dose) mosquito bites. Mortality in both groups was significantly higher than in uninfected controls, reaching 100% of high-dose birds and 90% of low-dose birds. Significant declines in food consumption and a corresponding loss of body weight occurred in malaria-infected birds. Both sex and body weight had significant effects on survival time, with males more susceptible than females and birds with low initial weights more susceptible than those with higher initial weights. Gross and microscopic lesions in malaria fatalities included massive enlargement of the spleen and liver, hyperplasia of the reticuloendothelial system with extensive deposition of malarial pigment, and overwhelming anaemia in which over 30% of the circulating erythrocytes were parasitized. Nutmeg Mannikins, by contrast, were completely refractory to infection. Our findings support previous studies documenting high susceptibility of native Hawaiian forest birds to avian malaria. This disease continues to threaten remaining high elevation populations of endangered native birds.

Hawai'i↗
USGS2013

Next-generation sequencing reveals cryptic mtDNA diversity of Plasmodium relictum in the Hawaiian Islands

Next-generation 454 sequencing techniques were used to re-examine diversity of mitochondrial cytochrome b lineages of avian malaria (Plasmodium relictum) in Hawaii. We document a minimum of 23 variant lineages of the parasite based on single nucleotide transitional changes, in addition to the previously reported single lineage (GRW4). A new, publicly available portal (Integroomer) was developed for initial parsing of 454 datasets. Mean variant prevalence and frequency was higher in low elevation Hawaii Amakihi (Hemignathus virens) with Avipoxvirus-like lesions (P = 0·001), suggesting that the variants may be biologically distinct. By contrast, variant prevalence and frequency did not differ significantly among mid-elevation Apapane (Himatione sanguinea) with or without lesions (P = 0·691). The low frequency and the lack of detection of variants independent of GRW4 suggest that multiple independent introductions of P. relictum to Hawaii are unlikely. Multiple variants may have been introduced in heteroplasmy with GRW4 or exist within the tandem repeat structure of the mitochondrial genome. The discovery of multiple mitochondrial lineages of P. relictum in Hawaii provides a measure of genetic diversity within a geographically isolated population of this parasite and suggests the origins and evolution of parasite diversity may be more complicated than previously recognized.

Hawai'i↗
USGS2014

How have fisheries affected parasite communities?

To understand how fisheries affect parasites, we conducted a meta-analysis of studies that contrasted parasite assemblages in fished and unfished areas. Parasite diversity was lower in hosts from fished areas. Larger hosts had a greater abundance of parasites, suggesting that fishing might reduce the abundance of parasites by selectively removing the largest, most heavily parasitized individuals. After controlling for size, the effect of fishing on parasite abundance varied according to whether the host was fished and the parasite's life cycle. Parasites of unfished hosts were more likely to increase in abundance in response to fishing than were parasites of fished hosts, possibly due to compensatory increases in the abundance of unfished hosts. While complex life cycle parasites tended to decline in abundance in response to fishing, directly transmitted parasites tended to increase. Among complex life cycle parasites, those with fished hosts tended to decline in abundance in response to fishing, while those with unfished hosts tended to increase. However, among directly transmitted parasites, responses did not differ between parasites with and without fished hosts. This work suggests that parasite assemblages are likely to change substantially in composition in increasingly fished ecosystems, and that parasite life history and fishing status of the host are important in predicting the response of individual parasite species or groups to fishing.

Parasitology↗
USGS2016

Concentration and retention of Toxoplasma gondii surrogates from seawater by red abalone (Haliotis rufescens)

Small marine snails and abalone have been identified as high- and low-risk prey items, respectively, for exposure of threatened southern sea otters to Toxoplasma gondii , a zoonotic parasite that can cause fatal encephalitis in animals and humans. While recent work has characterized snails as paratenic hosts for T. gondii , the ability of abalone to vector the parasite has not been evaluated. To further elucidate why abalone predation may be protective against T. gondii exposure, this study aimed to determine whether: (1) abalone are physiologically capable of acquiring T. gondii ; and (2) abalone and snails differ in their ability to concentrate and retain the parasite. Abalone were exposed to T. gondii surrogate microspheres for 24 h, and fecal samples were examined for 2 weeks following exposure. Concentration of surrogates was 2–3 orders of magnitude greater in abalone feces than in the spiked seawater, and excretion of surrogates continued for 14 days post-exposure. These results indicate that, physiologically, abalone and snails can equally vector T. gondii as paratenic hosts. Reduced risk of T. gondii infection in abalone-specializing otters may therefore result from abalone's high nutritional value, which implies otters must consume fewer animals to meet their caloric needs.

Parasitology↗