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Catalog of microscopic organisms of the Everglades, part 2—The desmids of the Arthur R. Marshall Loxahatchee National Wildlife Refuge

The Arthur R. Marshall Loxahatchee National Wildlife Refuge (refuge), Boynton Beach, Florida, contains approximately 147,000 acres southeast of Lake Okeechobee. Water quality in the interior portion of the refuge is strongly influenced by rainfall, resulting in slightly acidic waters with low dissolved ions. Desmids, a unique, ornate group of green algae loosely associated with submerged vascular plants, were photo-documented for the first time in samples from the refuge. The canal system surrounding the refuge contains a high level of ions from agricultural runoff, and intrusion of this water into the refuge interior during high canal water levels may have altered some of the desmid population. A transect from the canal to the interior was sampled every 3 months, and the species present were photographed, identified, and catalogued. Approximately 260 unique taxa from 29 genera were encountered. The interior of the refuge had the greatest diversity of desmids; however, the areas of the refuge adjacent to the canals still contained a rich population of desmids. We postulate that the diversity of desmids indicates that the pristine portions of the refuge may be an important refugium for desmids, particularly for those species restricted to the subtropical parts of the United States. This collection of taxa, identified to species with most specimens, will allow a more detailed examination of water quality issues when co-located water quality data are collected.

Florida

Assessment of the effect of road construction and other modifications on surface-water flow at St. Vincent National Wildlife Refuge, Franklin County, Florida

St. Vincent National Wildlife Refuge is managed by the U.S. Fish and Wildlife Service (USFWS). The refuge was acquired in 1968 from a private land owner and occupies all of St. Vincent Island, a barrier island located off the southern coast of the Florida Panhandle near Apalachicola (fig. 1). The island, which covers 12,358 acres, is about 9 miles long and 4 miles across at its widest point. Eighty miles of unpaved roads that grid the island are presently used for refuge management, law enforcement, and visitor hiking trails. Prior to becoming a refuge, the natural flow of surface water on the island was altered by road and ditch construction that enabled timbering of pine. Restoring the natural flow of surface water on the island to its historical state is one of the USFWS's goals for ecosystem restoration. During past road construction activities, fill was placed in the creeks to create raised roadbeds. This activity changed the natural flow of surface water by (1) acting as an earthen dam that impounded creeks; (2) restricting flow, thus increasing the depth of water in the channels of creeks; or (3) blocking the natural movement of saltwater in the creeks in coastal areas, thus altering water salinity. Along some sections of road, grading substantially lowered land-surface elevations. Along these sections of roads, adjacent creeks commonly flowed into one another during high-water conditions, thus allowing the transfer of water from one drainage basin to another. In some areas on the island, ditches were dug to manipulate the movement of surface water.

Florida

Ground-water contamination from lead shot at Prime Hook National Wildlife Refuge, Sussex County, Delaware

Prime Hook National Wildlife Refuge is located in southeastern Delaware in coastal lowlands along the margin of Delaware Bay. For 37 years, the Broadkiln Sportsman?s Club adjacent to the refuge operated a trap-shooting range, with the clay-target launchers oriented so that the expended lead shot from the range dropped into forested wetland areas on the refuge property. Investigators have estimated that up to 58,000 shotgun pellets per square foot are present in locations on the refuge where the lead shot fell to the ground. As part of the environmental risk assessment for the site, the U.S. Geological Survey (USGS) investigated the potential for lead contamination in ground water. Results from two sampling rounds in 19 shallow wells indicate that elevated levels of dissolved lead are present in ground water at the site. The lead and associated metals, such as antimony and arsenic (common shotgun pellet alloys), are being transported along shallow ground-water flowpaths toward an open-water slough in the forested wetland adjacent to the downrange target area. Water samples from wells located along the bank of the slough contained dissolved lead concentrations higher than 400 micrograms per liter, and as high as 1 milligram per liter. In contrast, a natural background concentration of lead from ground water in a well upgradient from the site is about 1 microgram per liter. Two water samples collected several months apart from the slough directly downgradient of the shooting range contained 24 and 212 micrograms per liter of lead, respectively. The data indicate that lead from a concentrated deposit of shotgun pellets on the refuge has been mobilized through a combination of acidic water conditions and a very sandy, shallow, unconfined aquifer, and is moving along ground-water flowpaths toward the surface-water drainage. Data from this study will be used to help delineate the lead plume, and determine the fate and transport of lead from the source area.

Water-Resources Investigations Report

A geospatial approach to identify water quality issues for National Wildlife Refuges in Oregon and Washington

Many National Wildlife Refuges (Refuges) have impaired water quality resulting from historic and current land uses, upstream sources, and aerial pollutant deposition. Competing duties limit the time available for Refuge staff to identify and evaluate potential water quality issues. As a result, water quality–related issues may not be resolved until a problem has already arisen. This study developed a geospatial approach for identifying and prioritizing water quality issues affecting natural resources (including migratory birds and federally listed species) within Refuge boundaries. We assessed the location and status of streams pursuant to the Clean Water Act in relation to individual Refuges in Oregon and Washington, United States. Although twelve Refuges in Oregon (60%) and eight Refuges in Washington (40%) were assessed under the Clean Water Act, only 12% and 3% of total Refuge stream lengths were assessed, respectively. Very few assessed Refuge streams were not designated as impaired (0% in Oregon, 1% in Washington). Despite the low proportions of stream lengths assessed, most Refuges in Oregon (70%) and Washington (65%) are located in watersheds with approved total maximum daily loads. We developed summaries of current water quality issues for individual Refuges and identified large gaps for Refuge-specific water quality data and habitat utilization by sensitive species. We conclude that monitoring is warranted on many Refuges to better characterize water quality under the Clean Water Act.

Oregon, Washington

Refuge management analyses: levee alternatives at Clarence Cannon National Wildlife Refuge

This report documents the results of a workshop that examined levee alternatives at Clarence Cannon National Wildlife Refuge. This refuge is located along the Mississippi River, approximately 50 miles north of St. Louis. It was purchased primarily for waterfowl maintenance. A levee providing flood protection from the Mississippi River has broken four times since the Refuge was acquired in 1965. A number of alternatives have been proposed in response to the most recent break in 1983. Long term alternatives considered at the workshop included: Improve the existing levee by raising the height approximately 2 ft. Improve the existing levee by raising the height approximately 2 ft. Maintain the levee at a lower elevation by including a spillway about 3 ft lower than the existing levee height. Do not maintain a levee so that the Refuge will flood directly with river stage. Repair of the major breaks in the levee, but not the more general wave damage, was considered as a short term alternative. Participants first specified the habitats and management controls desired at Clarence Cannon National Wildlife Refuge. These were centered around attaining the maximum feasible development and management of moist soil units. Levee alternatives were evaluated in terms of their ability to provide the desired habitats and management controls. Preliminary cost estimates were prepared for each alternative, and the qualitative consequences of each alternative identified for the full set of outputs from the Refuge Master Plan. The alternative of improving the existing levee by raising the height was unanimously preferred on purely “biological” grounds (with cost not considered). Repairing the levee with no change in elevation was unanimously selected as the most cost effective alternative.

Missouri

Nonbreeding duck use at Central Flyway National Wildlife Refuges

Within the U.S. portion of the Central Flyway, the U.S. Fish and Wildlife Service manages waterfowl on numerous individual units (i.e., Refuges) within the National Wildlife Refuge System. Presently, the extent of waterfowl use that Refuges receive and the contribution of Refuges to waterfowl populations (i.e., the proportion of the Central Flyway population registered at each Refuge) remain unassessed. Such an evaluation would help determine to what extent Refuges support waterfowl relative to stated targets, aid in identifying species requiring management attention, inform management targets, and improve fiscal efficiencies. Using historic monitoring data (1954–2008), we performed this assessment for 23 Refuges in Texas, New Mexico, Oklahoma, Kansas, and Nebraska during migration and wintering months (October–March). We examined six dabbling ducks and two diving ducks, plus all dabbling ducks and all diving ducks across two periods (long-term [all data] and short-term [last 10 October–March periods]). Individual Refuge use was represented by the sum of monthly duck count averages for October–March. We used two indices of Refuge contribution: peak contribution and January contribution. Peak contribution was the highest monthly count average for each October–March period divided by the indexed population total for the Central Flyway in the corresponding year; January contribution used the January count average divided by the corresponding population index. Generally, Refuges in Kansas, Nebraska, and New Mexico recorded most use and contribution for mallards Anas platyrhynchos . Refuges along the Texas Gulf Coast recorded most use and contribution for other dabbling ducks, with Laguna Atascosa and Aransas (including Matagorda Island) recording most use for diving ducks. The long-term total January contribution of the assessed Refuges to ducks wintering in the Central Flyway was greatest for green-winged teal Anas crecca with 35%; 12–15% for American wigeon Mareca americana , gadwall Mareca strepera , and northern pintail Anas acuta ; and 7–8% for mallard and mottled duck Anas fulvigula . Results indicated that the reliance on the National Wildlife Refuge System decreased for these ducks, with evidence suggesting that, for several species, the assessed Refuges may be operating at carrying capacity. Future analyses could be more detailed and informative were Refuges to implement a single consistent survey methodology that incorporated estimations of detection bias in the survey process, while concomitantly recording habitat metrics on and neighboring each Refuge.

Journal of Fish and Wildlife Management

Selenium concentrations in waters tributary to and in the vicinity of the Kesterson National Wildlife Refuge, Fresno and Merced counties, California

Analyses were made for selenium in waters and other materials of the Kesterson National Wildlife Refuge. Analyses were also made of source agricultural drainage waters from the San Luis Drain discharged into the refuge, and surrounding irrigation supply and return waters. Selenium concentrations range from 140 to 1 ,400 micrograms per liter (microgram/L) in irrigation drain waters supplied to the San Luis Drain. The selenium supplied to the Kesterson National Wildlife Refuge is to a small extent precipitated in sodium sulfate (thenardite) but a higher concentration was found in an alagal mat. Most other waters of the area contain less than detectable < 2 micrograms per liter concentrations of selenium. Oxidation of organic matter and reduction of selenate to selenite were found to be necessary for a quantitative analysis of total selenium. (USGS)

Water-Resources Investigations Report

Arctic National Wildlife Refuge, Alaska, Coastal Plain Resource Assessment: Report and recommendation to the Congress of the United States and final legislative environmental impact statement

The Arctic National Wildlife Refuge, in the northeastern corner of Alaska, was first established as the Arctic National Wildlife Range by Public Land Order 2214 in 1960, for the purpose of preserving unique wildlife, wilderness, and recreational values. The original 8.9-millionacre Range was withdrawn from all forms of appropriation under the public land laws, including mining laws but not including mineral leasing laws. This order culminated extensive efforts begun more than a decade earlier to preserve this unique part of Alaska. The following report analyzes the potential environmental consequences of five management alternatives for the coastal plain, ranging from opening for lease of the entire area for oil and gas development, to wilderness designation. A legislative environmental impact statement has been integrated into the report.

Alaska

Monitoring of wave, current, and sediment dynamics along the Fog Point Living Shoreline, Glenn Martin National Wildlife Refuge, Maryland

Living shorelines with salt marsh species, rock breakwaters, and sand nourishment were built along the coastal areas in the Glenn Martin National Wildlife Refuge, Maryland, in 2016 in response to Hurricane Sandy (2012). The Fog Point living shoreline at Glenn Martin National Wildlife Refuge was designed with the “headland - breakwater - embayment” pattern. Scientists from the U.S. Geological Survey, Northeastern University, U.S. Fish and Wildlife Service, and Louisiana State University studied wave, current, and sediment dynamics to assess the effectiveness of the Fog Point living shoreline structures in terms of wave attenuation and erosion reduction. Wave gages, current meters, sediment traps, sediment tiles, and lateral erosion pins were deployed along the Fog Point shoreline during February 10–14, 2020. Because of COVID-19 pandemic travel restrictions, sensors were not retrieved until August 25, 2021, which was 18 months after field deployment, resulting in tremendous loss or damage of sensors and sediment measurements. Monitoring data indicated that wave heights were substantially reduced at locations behind the breakwater (headland) compared to the wave heights in the offshore location, but not at the location in the control area (the embayment). Current patterns and current velocities at the location behind the breakwater were complex and changed dramatically compared to the current patterns and current velocities offshore. Sediments were blocked by the breakwater most of the time except during periods of storms with wave heights larger than 0.9 meter, when waves overtopped the breakwater and brought sediments to the tidal flat and salt marshes behind the breakwater. Behind the breakwater, both sediment deposition and erosion were observed during the 18 months of monitoring. Continued low elevation marsh edge erosion from wave undercutting along the embayment was observed, especially at the existing wave-cut gullies. Monitoring results indicate that the “breakwater + marsh planting” structure along the Fog Point shoreline has limited shoreline protection capacity. Marsh edge erosion behind the breakwater was likely caused by the limited sediment supply from marine sources for transport and delivery, as well as the effects of circulation and current velocity on the settling and deposition of suspended sediments from eroded marshes. Marsh edge erosion continued in the embayment or control area where no shoreline restoration structures were implemented. Long-term (decadal scale) monitoring and adaptive management of living shoreline structures could help to assess the effectiveness of wave attenuation for reducing shoreline erosion and enhancing vegetation growth for trapping sediments and the effectiveness of marsh surface elevation growth for keeping pace with sea level rise.

Maryland

Reconnaissance investigation of water-quality, bottom sediment, and biota associated with irrigation drainage in the Lower Rio Grande Valley and Laguna Atascosa National Wildlife Refuge, Texas, 1986-87

In 1986, the Department of the Interior conducted reconnaissance investigations in nine areas of the western conterminous United States to determine whether irrigation drainage has caused or has the potential to cause harmful effects to human health, fish, and wildlife, or may adversely affect the suitability of water for beneficial uses. Data collected in the lower Rio Grande valley and Laguna Atascosa National Wildlife Refuge reconnaissance investigation indicate that concentrations of dissolved minor elements in water are small. The maximum dissolved concentrations of arsenic, cadmium, mercury, chromium, selenium, and zinc exceed the 75th-percentile baseline values developed for the study; however, maximum dissolved concentrations of cadmium, mercury, and selenium exceeded the 75th-percentile baseline values by 1 microgram per liter or less. Concentrations of dissolved boron increased significantly from west to east. The smallest concentration of dissolved boron, 220 micrograms per liter, was detected in International Falcon Reservoir. The largest concentration of dissolved boron, 11,000 micrograms per liter, was detected on the refuge in Athel Pond. No chlorophenoxy herbicides were detected in water during the June 1986 sampling. Simazine, prometone, and atrazine were the only triazine herbicides detected, and concentrations of these herbicides did not exceed 0.8 microgram per liter. DDE, the only organochlorine Insecticide detected in water, was detected at two locations at concentrations of 0.01 micrograms per liter. Methyl parathion, malathion, and diazinon were the only organophosphorus compounds detected in the June 1986 sampling, and the maximum concentrations of these compounds were 0.75, 0.71, and 0.26 micrograms per liter, respectively. The analysis of three samples collected in August 1986 indicate that the types of pesticides collected during runoff were similiar to those detected during the June 1986 sampling. The exception was that the herbicide 2,4-D was detected during runoff. Concentrations of dissolved cadmium exceeded the chronic criteria for freshwater aquatic life in the Cayo Atascoso in the Laguna Atascosa National Wildlife Refuge. Chromium exceeded the acute and chronic freshwater criteria at four locations in the refuge and in the Laguna Madre. Chromium also exceeded the chronic saltwater criteria in Athel Pond. Concentrations of dissolved copper exceeded the acute and chronic criteria for saltwater aquatic life at 13 locations. Mercury exceeded the chronic criteria for freshwater and saltwater aquatic life at three locations, and dissolved nickel concentrations exceeded the chronic criteria for saltwater aquatic life in the Rio Grande at Anzalduas Dam and in the Resaca de los Frenos near Russeltown. No organophosphorus insecticides, polychlorinated napthalenes, or polychlorinated biphenyl compounds were detected in four bed-sediment samples. DDE, an organochlorine insecticide, was detected in all four samples at concentrations ranging from 0.2 to 34 micrograms per kilogram. Chlordane, ODD, DDE, DOT, and dieldrin were all detected in the Resaca de los Fresnos at U.S. Highway 77 at San Benito with concentrations of 4.0, 9.7, 9.3, 7.3, and 0.1 micrograms per kilogram, respectively. Data collected by U.S. Fish and Wildlife Service in 1985 indicate that DDE was detected in approximately 75 percent of the bed sediment samples analyzed. The maximum concentration detected in that study was 6.0 micrograms per gram; the median concentration was 0.01 micrograms per gram. Minor-element data from 22 fish samples indicate that the maximum concentrations of arsenic, copper, mercury, selenium, and zinc exceeded the 85thpercentile baseline concentrations established by the U.S. Fish and Wildlife Service for the National Contaminant Biomonitoring Program. None of the median concentrations of these minor elements exceeded the baseline concentrations. The maximum concentrations of aluminum, barium, iron, manganese, and tin were detected in fish collected from International Falcon Reservoir. This reservoir stratifies in the summer, and minor elements may be released from the bed sediments in the deep parts of the reservoir and incorporated into the food chain. Toxaphene was detected in 11 fish samples; detectable concentrations ranged from 0.98 to 5.1 micrograms per gram, wet weight. DOT also was detected in 11 fish samples with concentrations ranging from 0.021 to 0.066 micrograms per gram, wet weight. ODD was detected in 21 fish samples; concentrations ranged from 0.015 to 0.16 micrograms per gram, wet weight. DDE was detected in all 22 fish samples, and concentrations ranged from 0.36 to 9.9 micrograms per gram, wet weight. The maximum concentrations of DOT and ODD exceeded the 1980-81 baseline concentrations. The median and maximum concentrations of toxaphene and DDE exceeded the 1980-81 baseline concentrations. The largest concentrations of toxaphene, ODD, and DDE in fish were all measured in samples collected at the Main Floodway near Progreso.

Texas

Summary of bird-survey and banding results at W.L. Finley National Wildlife Refuge, 1998-2008

With some of the best remaining examples of oak habitats in the Willamette Valley, the Willamette Valley National Wildlife Refuge Complex (WVNWRC) has been implementing restoration efforts to reverse the successional trend towards Douglas-fir and maple that is threatening existing oak woodlands. The restoration work has been considered a model for other public and private efforts within the Willamette Valley, and has been showcased through the Oregon Oak Communities Working Group (http://www.oregonoaks.org). Although many oak restoration projects have been initiated over the last several years, and grant recipients typically identify wildlife species that are likely to benefit from their project, measures of success have not included the actual response of wildlife, such as a change in the probability of species occurrence or abundance. Monitoring in the WVNWRC has so far been limited to vegetative and structural changes within the plant community. Hagar and Stern (2001) identified bird species occurring in Willamette Valley oak woodlands that might be expected to benefit from such restoration efforts, including an endemic subspecies of the White-breasted Nuthatch (see Appendix 1 for scientific names of bird and plant species listed in this document), and the Acorn Woodpecker, both of which are species of concern in Oregon. However, empirical data documenting responses of bird assemblages to restoration actions are needed. The goal of this study was to document the effects of a restoration project in an Oregon White Oak woodland on Pigeon Butte in the W.L. Finley National Wildlife Refuge. Restoration treatments on Pigeon Butte include the removal of shade-tolerant tree species (primarily big-leaf maple and Douglas-fir) to reduce competition with oak trees and to return the stand to a more open structure. The objectives of this ongoing study are to compare abundance, survival, and productivity of diurnal songbird species before and after application of these restoration treatments. Monitoring these vital rates will provide crucial information about the effects of management on survival and productivity (DeSante and Rosenberg, 1998). Therefore, a constant-effort mist-netting project was continued in 2007 and 2008 that had previously collected songbird demographic data at Pigeon Butte from 1998 to 2002. Point-count surveys were conducted in the woodland to build on historical data available for the site (Anderson, 1970; Hagar and Stern, 2001). The data reported here represent 5 years of point count surveys and 6 years of banding before restoration treatment, but only one post-treatment sampling season. Continued monitoring of the bird population is recommended to determine both short-term effects and long-term trends following the habitat alterations that result from restoration treatment.

Oregon

A comparison of contemporary and historical hydrology and water quality in the foothills and coastal plain of the Arctic National Wildlife Refuge, Arctic Slope, northern Alaska

The Arctic National Wildlife Refuge is a unique landscape in northern Alaska with limited water resources, substantial biodiversity of rare and threatened species, as well as oil and gas resources. The region has unique hydrology related to perennial springs, and the formation of large aufeis fields—sheets of ice that grow in the river channels where water reaches the surface in the winter and freezes. This work aims to update our understanding of water resources and water quality in the springs, streams, rivers, and lakes of this region, returning to sites sampled by the U.S. Geological Survey in the 1970s. We resampled eight streams, four springs, and six lakes for hydrological metrics, water quality, and macroinvertebrates, and recalculated flood-frequency metrics for rivers using updated data and modern techniques. Aufeis field melt rates were also assessed for the past several decades. Although the available data preclude trend determinations in most cases, our analysis and comparison to the historical sampling indicates an increase in dissolved ions for streams and springs, faster and earlier aufeis melt, and similar macroinvertebrate populations.

Alaska

Wetland modeling and information needs at Stillwater National Wildlife Refuge

The marshes in and around Stillwater National Wildlife Refuge (the Refuge) are extremely dynamic; expanding and contracting in size both seasonally, due to runoff and subsequent evapotranspiration, and over longer periods, due to climatic variation. The dynamic nature of these marshes results in a diversity of wetland habitats, which support a variety of migratory birds. To maintain this wetland diversity and control the loss of migratory bird habitat in the Lahontan Valley, the Refuge was established and currently manages a complex of marsh units. However, changes in the hydrology, and changes that will occur as a result of the Fallon Paiute-Shoshone and Truckee-Carson-Pyramid Lake Water Rights Settlement Act (Public Law 101-618, 104 Stat. 3389), greatly affect the Refuge's wetland management capability. In light of these changes, and the legal requirements associated with environmental impact assessments, the Refuge convened a workshop to discuss several aspects of wetland management in the Lahontan Valley. The workshop, described in this report, had three primary objectives: 1. discuss the types and relative proportions of primary wetland habitats that should be provided as described in the settlement act; 2. discuss wetland management models that might be developed to help manage these marshes under hydrologic regimes likely in the future; and 3. discuss future information and monitoring needs, including proposals for valley-wide biodiversity surveys, which would be helpful when considering withdrawn Bureau of Reclamation (BR) lands for possible incorporation into the Refuge. Several presentations at the beginning of the workshop provided a common basis for discussing these objectives. Refuge staff provided background on the history and past management. The Nature Conservatory discussed their role in the settlement act, proposals for valley-wide biodiversity surveys, and results of a literature review for Stillwater Marsh and the Lahontan Valley (Nachlinger 1993). Kay Fowler provided an historical context of changes in vegetation and waterbird use of the marshes based on her ethnography of the local Paiute Indians (Fowler 1993). Finally, Bob Elston discussed a model that predicts archaeological sites based on environmental variables (Raven and Elston 1989). The workshop was organized by staff from the Refuge and facilitated by the authors of this report. Participants included Ron Anglin, Bill Henry, Anne Janik, Cliff Creger, Fred Paveglio, and Mary Jo Elpers of the U.S. Fish and Wildlife Service (the Service); Jeff Baumgartner, Jan Nachlinger, Hope Humphries, and Graham Chisholm of The Nature Conservancy; David Yardas of the Environmental Defense Fund; David Robertson of Robertson Software, Inc.; Norm Saake, Terry Retterer, and Larry Neel of the Nevada Department of Wildlife; Lew Oring and Kay Fowler of the University of Nevada; and Robert Elston of Intermountain Research.

Report

Monitoring and assessment of juvenile steelhead on Toppenish National Wildlife Refuge. Quick Response Project for 2001

Toppenish National Wildlife Refuge (TNWR) in south central Washington was established in 1964 to provide an important link in the chain of feeding and resting areas for waterfowl and other migratory birds using the Pacific Flyway. Wetlands on TNWR include both natural floodplain wetlands along Toppenish Creek and man-made impoundments designed to mimic natural floodplain processes. Wetland improvement projects were completed between 1995-1998 to restore wetland habitat conditions and eliminate monotypic stands of invasive reed canary grass. Habitat improvements boosted fall/winter use from 2,000 to 50,000 waterfowl, and increased overall use by many species of water birds, shorebirds, and other migratory birds. Increased use by bald eagles, peregrine falcons, and willow flycatchers has also been recorded.

Washington

Additions to the aquatic diptera (Chaoboridae, Chironomidae, Culicidae, Tabanidae, Tipulidae) fauna of the White River National Wildlife Refuge, Arkansas

The dipteran fauna of Arkansas is generally poorly known. A previous study of the Aquatic macroinvertebrates of the White River National Wildlife Refuge, the largest refuge in Arkansas, reported only 12 diptera taxa out of 219 taxa collected (Chordas et al., 1996). Most of the dipterans from this study were identified only to the family level. The family Chironomidae is a large, diverse group and was predicted to be much more diverse in the refuge than indicated by previous studies. In this study, Chironomidae were targeted, with other aquatic or semiaquatic dipterans also retained, in collections designed to better define the dipteran fauna of the White River National Wildlife Refuge. Adult dipterans were collected from 22 sites within the refuge using sweep-nets, two types of blacklight traps, and lighted fan traps in June of 2001. Specimens from previous studies were retrieved and identified to the lowest possible taxonomic level. A total of 4,917 specimens representing 122 taxa was collected. The 122 taxa were comprised of the following: two chaoborids, 83 chironomids, 15 culicids, nine tabanids, and 13 tipulids. Of these, 46 species are new state records for Arkansas. Nine undescribed species of chironomids were collected, and eight species records represent significant range extensions.

Journal of the Arkansas Academy of Science

Eelgrass (Zostera marina) and Seaweed Abundance along the Coast of Togiak National Wildlife Refuge, Alaska, 2008–10

We conducted a point-sampling survey to determine eelgrass ( Zostera marina ) and seaweed abundance in coastal waters adjacent to Togiak National Wildlife Refuge, Alaska, in July 2008–10. Eelgrass was known to be abundant in protected embayments of the southeastern Bering Sea and near the Togiak National Wildlife Refuge, but prior to this study, no systematic ground surveys had been conducted in these areas. We determined mean aboveground biomass of eelgrass to be highly variable among years observed, ranging from 32–72 grams dry weight per square meter (g/m 2 ) during successive years in Nanvak Bay and among the studied embayments in 2010: 47±4 g/m 2 in Nanvak Bay, 69±7 g/m 2 in Chagvan Bay, and 74±15 g/m 2 in Goodnews Bay. Seaweed density, abundance, and frequency scores were also highly variable among years and among embayments and were lower for seaweeds than for eelgrass in Nanvak and Chagvan bays, but not in Goodnews Bay. For all bays, mussels ( Mytilus spp.) and gastropods were the most common macro-invertebrates detected during surveys, whereas sea stars, crabs, and sponges were not observed in the embayments.

Alaska

Detection probabilities and site occupancy estimates for amphibians at Okefenokee National Wildlife Refuge

We conducted an amphibian inventory at Okefenokee National Wildlife Refuge from August 2000 to June 2002 as part of the U.S. Department of the Interior's national Amphibian Research and Monitoring Initiative. Nineteen species of amphibians (15 anurans and 4 caudates) were documented within the Refuge, including one protected species, the Gopher Frog Rana capito. We also collected 1 y of monitoring data for amphibian populations and incorporated the results into the inventory. Detection probabilities and site occupancy estimates for four species, the Pinewoods Treefrog (Hyla femoralis), Pig Frog (Rana grylio), Southern Leopard Frog (R. sphenocephala) and Carpenter Frog (R. virgatipes) are presented here. Detection probabilities observed in this study indicate that spring and summer surveys offer the best opportunity to detect these species in the Refuge. Results of the inventory suggest that substantial changes may have occurred in the amphibian fauna within and adjacent to the swamp. However, monitoring the amphibian community of Okefenokee Swamp will prove difficult because of the logistical challenges associated with a rigorous statistical assessment of status and trends.

American Midland Naturalist

Reconnaissance investigation of water quality, bottom sediment, and biota associated with irrigation drainage in Bowdoin National Wildlife Refuge and adjacent areas of the Milk River basin, northeastern Montana, 1986-87

Concentrations of trace elements, radiochemicals, and pesticides in the Bowdoin National Wildlife Refuge lakes generally were not substantially larger than those in the water supplied from Dodson South Canal or in irrigation drainage. Concentrations of arsenic (47 micrograms/L), uranium (43 microg/L), and vanadium (51 microg/L) in Dry Lake Unit, and boron (1,000 microg/L) in Lake Bowdoin were notably larger than at other sites. Zinc concentrations in an irrigation drain (56 microg/L) and two shallow domestic wells (40 and 47 microg/L) were elevated relative to other sites. Concentrations of gross alpha radiation (64 picocuries/L) and gross beta radiation (71 picocuries/L) were elevated in Dry Lake Unit. Pesticides concentrations at all sites were 0.08 microg/L or less. Water use guidelines concentrations for boron, cadmium, uranium, zinc, and gross alpha radiation were slightly exceeded at several sites. In general, trace-constituent concentrations measured in the water do not indicate any potential toxicity problems in Bowdoin National Wildlife Refuge; however, highwater conditions in 1986 probably caused dilution of dissolved constituents compared to recent dry years. Trace element concentrations in bottom sediments of the refuge lakes were generally similar to background concentrations in the soils. The only exception was Dry Lake Unit, which had concentrations of chromium (99 micrograms/g), copper (37 microg/g), nickel (37 microg/g), vanadium (160 microg/g), and zinc (120 microg/g) that were about double the mean background concentrations. The maximum selenium concentration in bottom sediment was 0.6 microg/g. Pesticide concentrations in bottom sediments were less than analytical detection limits at all sites. With few exceptions, concentrations of trace elements and pesticides in biota generally were less than values known to produce harmful effects on growth or reproduction.

Montana