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The Palmyra Atoll Research Consortium

Palmyra Atoll in the tropical Pacific is the site of some exciting work by scientists from a diverse collection of research institutions. The Palmyra Atoll Research Consortium (PARC) fosters collaborative multi- and inter-disciplinary research by U.S. Department of the Interior (DOI) agencies (USGS and U.S. Fish and Wildlife Service–USFWS), academic institutions (for example, Stanford University, University of California, and Victoria University of Wellington in New Zealand) and non-governmental organizations (for example, American Museum of Natural History and The Nature Conservancy) on the terrestrial and marine ecosystems at Palmyra Atoll. USGS has been a member of PARC since its inception in 2004. Palmyra functions as a living laboratory. It is a low-lying coral atoll located about 1,800 kilometers south/southwest of Hawaii near the equator in the central Pacific Ocean (latitude 5°53'N, longitude 162°05'W). Palmyra Atoll and nearby Kingman Reef are U.S. territories and represent the northern atolls/reefs of the U.S. Line Islands. Palmyra also is one of the nine sovereign territories of the United States commonly referred to as the U.S. Pacific Remote Island Areas (PRIAs) that straddle the equator (fig. 1). Palmyra Atoll and nearby Kingman Reef also were included as part of the seven territories that comprise the Pacific Remote Islands Marine National Monument set aside by President Bush in 2009 (Proclamation 8336), which includes the same territories as the PRIAS, except Rose Atoll and Midway Atoll.

Palmyra Atoll↗

Understanding pesticides in California's Delta

The Sacramento-San Joaquin River Delta (Delta) is the hub of California’s water system and also an important habitat for imperiled fish and wildlife. Aquatic organisms are exposed to mixtures of pesticides that flow through the maze of Delta water channels from sources including agricultural, landscape, and urban pest-control applications. While we do not know all of the effects pesticides have on the ecosystem, there is evidence that they cause some damage to organisms in the Delta. Decades of USGS research have provided a good understanding of when, where, and how pesticides enter the Delta. However, pesticide use is continually changing. New field studies and methods are needed so that scientists can analyze which pesticides are present in the Delta, and at what concentrations, enabling them to estimate exposure and ultimate effects on organisms. Continuing research will provide resource managers and stakeholders with crucial information to manage the Delta wisely.

California↗

Assessment of ecosystem response to a temporary water level drawdown and subsequent refilling at Topock Marsh, Arizona—July 2011–October 2014

Topock Marsh is a 1,637-hectare (4,045-acre) wetland adjacent to the Colorado River near Needles, California, and a main feature of Havasu National Wildlife Refuge (NWR). The U.S. Fish and Wildlife Service, in cooperation with the Bureau of Reclamation, began construction of an infrastructure improvement project in 2010 to increase the efficiency of water use and to help protect the habitats and species found within the Havasu NWR. During construction, normal water delivery from the Colorado River into Topock Marsh through the Inlet Canal was restricted, which resulted in unusually low water elevations in 2011. The U.S. Geological Survey, commissioned by the U.S. Fish and Wildlife Service, undertook the investigation of the water quality and aquatic flora and fauna during the low water conditions. Subsequently, water elevations in the marsh returned to more normal elevations after the new concrete-lined Fire Break Canal became fully operational in January 2012. The U.S. Geological Survey made 11 field trips to the Havasu NWR between July 2011 and October 2014 to assess the effects of the temporary low water conditions and the change of inflow location (from the Inlet Canal to the Fire Break Canal) on water quality and aquatic habitat. The following conditions were monitored: water quality, sediment and plant chemistry, phytoplankton, zooplankton, aquatic macro-invertebrates, and emergent and submerged aquatic vegetation (SAV). Water-quality and biota data collected during 2013–14 were then compared with data collected during the 2011–12 low water period. Once the new Fire Break Canal became operational and Colorado River water flowed regularly into the marsh, concentrations of several water quality parameters decreased (for example, specific conductance, total dissolved solids, turbidity, chlorophyll a , and total and organic nitrogen), and phytoplankton abundance was reduced at the upstream sampling stations (TP-3, TP-2, and TP-6); the water flow pushed water with higher concentrations of these components downstream (measured at TP-8). The upstream sampling locations in 2013–14 had decreased turbidity, therefore more SAV biomass accumulated, especially in shallow areas with water depths of ≤1.0 meter (≤3.3 feet). However, the furthest downstream station had higher turbidity caused by both the suspension of autochthonous sediment and high phytoplankton density and biovolume. This higher turbidity resulted in minimal SAV growth, especially in the deeper water (>1.0 meter [>3.3 feet]). Emergent vegetation not only survived the low water conditions of 2011, but expanded its areal coverage and subsequently thrived in the higher water elevations. Overall, no immediate critically negative consequences were detected for aquatic fauna or flora that could be attributd unequivocally to the effect of low water levels. Concentrations of nutrient and trace elements in all water samples were below wildlife toxicity thresholds as established by Arizona Department of Environmental Quality. Three nonnative species were discovered shortly after the Fire Break Canal went into operation. Of the three, gizzard shad ( Dorosoma cepedianum ) and Eurasian watermilfoil ( Myriophyllum spicatum ) increased substantially in numbers from 2011–14, but quagga mussels ( Dreissena bugensis ) did not increase. Future monitoring will determine the long-term impact of the new flow regime

Arizona↗

A taping method for external transmitter attachment on aquatic snakes

Radio telemetry is extremely useful for studying habitat use and movements of free ranging snakes. Surgically implanting radio transmitters into the body cavity of snakes is standard practice in most studies (e.g., Reinert and Cundall 1982; Weatherhead and Blouin-Demers 2004), but this implanting method has its drawbacks. Surgery itself is risky for individual snakes because of the potential for infection or incomplete healing of the incision site. Also, transmitters that are small enough to be carried by small or slender snakes have a relatively short battery life and need to be removed or replaced often, thus requiring frequent surgeries. In rare or endangered snake species, the risk of using invasive implantation surgery may not be merited. External attachment methods are relatively non-invasive and allow removal and replacement of radio transmitters on smaller snakes. The Giant Gartersnake ( Thamnophis gigas ) is a semi-aquatic snake endemic to wetlands of the Central Valley of California, USA, and is federally and state listed as threatened (U.S. Fish and Wildlife Service 1999). Telemetry studies of the habitat use and movements of this species typically used surgically implanted radio transmitters, but this method is limited to larger snakes, primarily females, because of size requirements for surgery (> 250 g). To overcome difficulties and biases associated with radio telemetry of T. gigas , we developed and evaluated several alternative techniques to attach external radio transmitters using tape.

California↗

A conceptual model for site-level ecology of the giant gartersnake ( Thamnophis gigas ) in the Sacramento Valley, California

Giant gartersnakes (Thamnophis gigas) comprise a species of semi-aquatic snakes precinctive to marshes in the Central Valley of California (Hansen and Brode, 1980; Rossman and others, 1996). Because more than 90 percent of their historical wetland habitat has been converted to other uses (Frayer and others, 1989; Garone, 2007), giant gartersnakes have been listed as threatened by the State of California (California Department of Fish and Game Commission , 1971) and the United States (U.S. Fish and Wildlife Service, 1993). Giant gartersnakes currently occur in a highly modified landscape, with most extant populations occurring in the rice - growing regions of the Sacramento Valley, especially near areas that historically were tule marsh habitat (Halstead and others, 2010, 2014). In ricelands and managed marshes, many operational decisions likely affect the health and viability of giant gartersnake populations. Land-use decisions, including the management of water, aquatic vegetation, terrestrial vegetation, and co-occurring species, have the potential to affect giant gartersnakes. Little is known, however, about the effects of these types of decisions on the viability of giant gartersnake populations. Bayesian network models are a useful tool to help guide decisions with uncertain outcomes. These models require the articulation of what experts think they know about a system, and facilitate learning about the hypothesized relations (Marcot and others, 2001; Uusitalo , 2007). Bayesian networks further provide a clear visual display of the model that facilitates understanding among various stakeholders (Marcot and others, 2001; Uusitalo , 2007). Empirical data and expert judgment can be combined, as continuous or categorical variables, to update knowledge about the system (Marcot and others, 2001; Uusitalo , 2007). Importantly, Bayesian network models allow inference from causes to consequences, but also from consequences to causes, so that data can inform the states of nodes (values of different random variables) in either direction (Marcot and others, 2001; Uusitalo , 2007). Because they can incorporate both decision nodes that represent management actions and utility nodes that quantify the costs and benefits of outcomes, Bayesian networks are ideally suited to risk analysis and adaptive management (Nyberg and others, 2006; Howes and others, 2010). Thus, Bayesian network models are useful in situations where empirical data are not available, such as questions concerning the responses of giant gartersnakes to management.

California↗

Ecology of Greater Sage-Grouse in the Bi-State Planning Area Final Report, September 2007

Conservation efforts for greater sage-grouse (Centrocercus urophasianus), hereafter sage-grouse, are underway across the range of this species. Over 70 local working groups have been established and are implementing on-the-ground sage-grouse oriented conservation projects. Early on in this process, the California Department of Fish and Game (CDFG) recognized the need to join in these efforts and received funding from the U.S. Fish and Wildlife Service (USFWS) under the Candidate Species Conservation Program to help develop a species conservation plan for sage-grouse in the Mono County area. This conservation plan covers portions of Alpine, Mono, and Inyo counties in California and Douglas, Esmeralda, Lyon, and Mineral counties in Nevada. A concurrent effort underway through the Nevada Governor's Sage-grouse Conservation Team established Local Area Working Groups across Nevada and eastern California. The Mono County populations of sage-grouse were encompassed by the Bi-State Local Planning Area, which was comprised of six population management units (PMUs). The state agencies from California (CDFG) and Nevada (Nevada Department of Wildlife; NDOW) responsible for the management of sage-grouse agreed to utilize the process that had begun with the Nevada Governor's Team in order to develop local plans for conservation planning and implementation. Resources from the USFWS were applied to several objectives in support of the development of the Bi-State Local Area Sage-grouse Conservation Plan through a grant to the U.S. Geological Survey (USGS). Objectives included: (1) participate in the development of the Bi-State Conservation Plan, (2) compile and synthesize existing sage-grouse data, (3) document seasonal movements of sage-grouse, (4) identify habitats critical to sage-grouse, (5) determine survival rates and identify causal factors of mortality, (6) determine nest success and brood success of sage-grouse, and (7) identify sage-grouse lek sites. Progress reports completed in 2004 and 2005 addressed each of the specific objectives and this final report focuses on the biological information gathered in support of local conservation efforts. Participation in the development of the Bi-State Local Area Conservation Plan was accomplished on multiple scales. Beginning in the fall of 2002, USGS personnel began participating in meetings of local stakeholders involved in the development of a sage-grouse conservation plan for the Bi-State planning area. This included attendance at numerous local PMU group meetings and field trips as well as participating on the technical advisory committee (TAC) for the Bi-State group. Whenever appropriate, ongoing results and findings regarding sage-grouse ecology in the local area were incorporated into these working group meetings. In addition, the USGS partnered with CDFG to help reorganize one of the local PMU groups (South Mono) and edited that portion of the Bi-State plan. The USGS also worked closely with CDFG to draft a description of the state of knowledge for sage-grouse genetic information for inclusion in the Bi-State Conservation Plan. The first edition of the Bi-State Conservation Plan for Greater Sage-Grouse was completed in June 2004 (Bi-State Sage-grouse Conservation Team 2004). This report is organized primarily by PMU to facilitate the incorporation of these research findings into the individual PMU plans that compose the Bi-State plan. Information presented in this report was derived from over 7,000 radio-telemetry locations obtained on 145 individual sage-grouse during a three year period (2003-2005). In addition, we collected detailed vegetation measurements at over 590 habitat sampling plots within the study area including canopy cover, shrubs, forbs, and grasses diversity. Vegetation data collection focused on sage-grouse nests, and brood-use areas. Additionally we collected data at random sites to examine sage-grouse habitat relationships within the study area. The majori

Open-File Report↗

Biomonitoring of Environmental Status and Trends (BEST) Program: Environmental contaminants, health indicators, and reproductive biomarkers in fish from the Colorado River basin

Seven fish species were collected from 14 sites on rivers in the Colorado River Basin (CDRB) from August to October 2003. Spatial trends in the concentrations of accumulative contaminants were documented and contaminant effects on the fish were assessed. Sites were located on the mainstem of the Colorado River and on the Yampa, Green, Gunnison, San Juan, and Gila Rivers. Common carp (Cyprinus carpio), black bass (Micropterus sp.), and channel catfish (Ictalurus punctatus) were the targeted species. Fish were field-examined for external and internal anomalies, selected organs were weighed to compute somatic indices, and tissue and fluid samples were preserved for fish health and reproductive biomarker analyses. Composite samples of whole fish, grouped by species and gender, from each site were analyzed for organochlorine and elemental contaminants using performance-based and instrumental methods. 2,3,7,8-tetrachlorodibenzo-p-dioxin-like activity (TCDD-EQ) was measured using the H4IIE rat hepatoma cell bioassay. Selenium (Se) and mercury (Hg) concentrations were elevated throughout the CDRB, and pesticides concentrations were greatest in fish from agricultural areas in the Lower Colorado River and Gila River. Selenium concentrations exceeded toxicity thresholds for fish (>1.0 ?g/g ww) at all sites except from the Gila River at Hayden, Arizona. Mercury concentrations were elevated (>0.1 ?g/g ww) in fish from the Yampa River at Lay, Colorado; the Green River at Ouray National Wildlife Refuge (NWR), Utah and San Rafael, Utah; the San Juan River at Hogback Diversion, New Mexico; and the Colorado River at Gold Bar Canyon, Utah, Needles, California, and Imperial Dam, Arizona. Concentrations of p,p'-DDE were relatively high in fish from Arlington, Arizona (>1.0 ?g/g ww) and Phoenix, Arizona (>0.5 ?g/g ww). Concentrations of other banned pesticides including toxaphene, total chlordanes, and dieldrin were also greatest at these two sites but did not exceed toxicity thresholds. Current-use or unlisted pesticides such as dacthal, endosulfan, '-HCH, and methoxychlor were also greatest in fish from Gila River. Total polychlorinated biphenyls (PCBs; >0.11 ?g/g ww) and TCDD-EQs (>5 pg/g ww) exceeded wildlife guidelines in fish from the Gila River at Phoenix, Arizona. Hepatic ethoxyresorufin O-deethylase (EROD) activity was also relatively high in carp from the Gila River at Phoenix, Arizona and in bass from the Green River at Ouray NWR, Utah. Altered biomarkers were noted in fish throughout the CDRB. Fish from some stations responded to chronic contaminant exposure as indicated by fish health and reproductive biomarker results. Multiple fish health indicators including altered body and organ weights and high health assessment index scores may be associated with elevated Se concentrations in fish from the Colorado River at Loma, Colorado and Needles, California. Although grossly visible external or internal lesions were found on most fish from some sites, histopathological analysis determined many of these to be inflammatory responses associated with parasites. Edema, exophthalmos, and cataracts were noted in fish from sites with elevated Se concentrations. Reproductive biomarkers including gonad development and maturation, vitellogenin concentrations, and steroid hormone concentrations were anomalous in fish from the Gila River at Hayden and Phoenix, Arizona. In addition, intersex fish were found at seven of 14 sites. The intersex condition was identified in smallmouth bass (M. dolomieu), largemouth bass (M. salmoides), channel catfish, and carp and may indicate exposure to endocrine disrupting compounds. Seven of ten male smallmouth bass from the Yampa River at Lay, Colorado were intersex. Male carp, bass, and channel catfish with low concentrations of vitellogenin were common in the CDRB. Comparatively high vitellogenin concentrations (>0.2 mg/mL) were measured in male fish from the Green River at Ouray NWR, Utah and the Colorado River at Im

Scientific Investigations Report↗

USGS science at work in the San Francisco Bay and Sacramento-San Joaquin Delta estuary

The San Francisco Bay and Sacramento-San Joaquin Delta form one of the largest estuaries in the United States. The “Bay-Delta” system provides water to more than 25 million California residents and vast farmlands, as well as key habitat for birds, fish, and other wildlife. To help ensure the health of this crucial estuary, the U.S. Geological Survey, in close cooperation with partner agencies and organizations, is providing science essential to addressing societal issues associated with water quantity and quality, sediment transportation, environmental contamination, animal health and status, habitat restoration, hazards, ground subsidence, and climate change.

California↗

Transcontinental mourning dove recovery

A Mourning Dove ( Zenaida macroura ) banded in New York has been reported shot in California. On 25 August 1969, near Palmyra (43 ° 00' N, 77 ° 10' W), New York Department of Environmental Conservation personnel placed U. S. Fish & Wildlife Service band 883-97279 on the leg of a hatching-year Mourning Dove of unknown sex. During the first weekend of the dove season in September 1970, Stan Solus (P.O. Box 594, Seiad Valley, California) recovered the band from a dove he shot in the Shasta Valley, Siskiyou County, California (41 ° 30' N, 122 ° 20' W). As Mr. Solus included the band with his reporting letter and, in response to my asking him for verification, reaffirmed his original information, the recovery has been accepted as authentic. I suggest this vagrancy may be explained by assuming that the inexperienced New York bird got emotionally involved with a western bird with which it shared winter quarters, perhaps in Mexico, and thus the following year ended up a flower child in California.

The Auk↗

Black abalone surveys at Naval Base Ventura County, San Nicolas Island, California: 2019, annual report

The U.S. Geological Survey Western Ecological Research Center’s Santa Cruz Field Station, Santa Cruz, California, has been funded by the U.S. Navy to continue monitoring a suite of intertidal black abalone sites at San Nicolas Island, California. The nine rocky intertidal sites were established in 1980 by Glenn VanBlaricom (then of the U.S. Fish and Wildlife Service) to study the potential impact of translocated sea otters on the intertidal black abalone population at the island. The sites were monitored from 1981 to 1997, usually annually or semi-annually. Monitoring resumed in 2001, and regular annual monitoring cycles have been conducted at the sites since then. The study sites became particularly important, from a management perspective, after a virulent disease decimated black abalone populations throughout southern California beginning in the mid-1980s. The disease, withering syndrome, was first observed on San Nicolas Island in 1992 and during the next few years reduced the population there by approximately 99 percent. The species was subsequently listed as endangered under the Endangered Species Act in 2009. The subject of this report is the 2019 monitoring cycle of the sites and how the current status fits into the long-term data at San Nicolas Island. Since 2001, the monitored population has increased nearly tenfold to approximately 8.7 percent of the pre-disease level. This increase has resulted from generally higher levels of recruitment than seen in the first two decades of monitoring, punctuated by a few high recruitment events. Most of the population growth has been at two of the nine sites (sites 7 and 8). This pattern continued in 2019 with increasing numbers at sites 7 and 8 and the highest number of abalone counted and measured island-wide since 1996. However, counts declined at six of the sites during the last year and the increases in counts at sites 7 and 8 barely offset these losses. Recruitment rates have fallen since a peak in 2017 but 2019 continued to show some additional recruitment. The distance between adjacent black abalone, a metric relevant to potential reproduction, has decreased substantially since it was first consistently measured in 2005. Although sand burial can have devastating localized consequences to black abalone, the sand cover data we collected was not sufficient to suggest an obvious temporal or site-based pattern to sedimentation, and there is no indication that this was a factor in any of the declines recorded in 2019. Continued monitoring of these sites can provide island biologists with species trends to aid in adaptive management of the resource.

California↗

Status of selenium in south San Francisco Bay—A basis for modeling potential guidelines to meet National tissue criteria for fish and a proposed wildlife criterion for birds

The U.S. Environmental Protection Agency (EPA) proposed Aquatic Life and AquaticDependent Wildlife Criteria for Selenium (Se) in California’s San Francisco Bay and Delta (Bay-Delta) in June 2016. Here we apply the same modeling methodology—Ecosystem-Scale Selenium Modeling— to an assessment of conditions and documentation of food webs of south San Francisco Bay (South Bay) as an exploratory framework in support of site-specific Se criteria development. Long-term datasets contribute to the basis for modeling, especially the 21-year collection of the clam Macoma petalum from a mudflat at the lower end of South Bay (Lower South Bay). As such, this is a working document that may serve as a basis to establish an understanding of the specifics of Se biodynamics within the estuary and reduce uncertainties about how to protect it. This approach brings together the main factors involved in toxicity: likelihood of high exposure, inherent species sensitivity, and the behavioral ecology (for example, demographics and life history) of an organism in terms of susceptibility to a reproductive toxicant. Species sensitivity is represented here by use of the EPA’s current national tissue Se criterion for fish or that proposed to protect the eggs of aquatic birds for the Bay-Delta (U.S. Environmental Protection Agency, 2016a, 2016b, 2016c). This report also strives to bring together findings and field data across a body of literature for South Bay to provide an integrative assessment. We find an assemblage of site-specific conditions that could affect modeling: associated urban processes such as discharges from municipal wastewater treatment plants and drainage from mercury (Hg) mining and limestone extraction are sources of Se that characterize the Lower South Bay as the location of interest for Se exposure; • hydrodynamics are lagoon-like (that is, less flushing), which sustains elevated nutrients and phytoplankton blooms; • managed freshwater sources are a major hydrodynamic component; • birds, in addition to fish, are prominent predators in South Bay; • wetland restoration has recently intervened to play a significant role in ecosystem function that may include uptake of both Hg and Se; • the dietary food web of surficial-sediment to M. petalum is important because of the dominance of this clam species and its elevated Se bioaccumulation potential compared to other local food webs; and 2 • maximal Se concentrations may be limited by transitory or annually renewed food webs (for example, migratory shorebirds and decimation of clams from marshes). We also find that the constructed mechanistic model: • spatially connects to the Palo Alto mudflat site because of data availability; • accurately predicts average observed Se concentrations in M. petalum and in predator species of fish and birds; and • is able to bracket a range of potential protective water-column Se concentrations specific to predator species based on the EPA’s national Se criterion for whole-body fish tissue and a proposed site-specific criterion for bird eggs in the Bay-Delta.

California↗

Population dynamics of spotted owls in the Sierra Nevada, California

The California spotted owl (Strix occidentalis occidentalis) is the only spotted owl subspecies not listed as threatened or endangered under the United States Endangered Species Act despite petitions to list it as threatened. We conducted a meta-analysis of population data for 4 populations in the southern Cascades and Sierra Nevada, California, USA, from 1990 to 2005 to assist a listing evaluation by the United States Fish and Wildlife Service. Our study areas (from N to S) were on the Lassen National Forest (LAS), Eldorado National Forest (ELD), Sierra National Forest (SIE), and Sequoia and Kings Canyon National Parks (SKC). These study areas represented a broad spectrum of habitat and management conditions in these mountain ranges. We estimated apparent survival probability, reproductive output, and rate of population change for spotted owls on individual study areas and for all study areas combined (meta-analysis) using model selection or model-averaging based on maximum-likelihood estimation. We followed a formal protocol to conduct this analysis that was similar to other spotted owl meta-analyses. Consistency of field and analytical methods among our studies reduced confounding methodological effects when evaluating results. We used 991 marked spotted owls in the analysis of apparent survival. Apparent survival probability was higher for adult than for subadult owls. There was little difference in apparent survival between male and female owls. Model-averaged mean estimates of apparent survival probability of adult owls varied from 0.811 ?? 0.021 for females at LAS to 0.890 ?? 0.016 for males at SKC. Apparent survival increased over time for owls of all age classes at LAS and SIE, for adults at ELD, and for second-year subadults and adults at SKC. The meta-analysis of apparent survival, which included only adult owls, confirmed an increasing trend in survival over time. Survival rates were higher for owls on SKC than on the other study areas. We analyzed data from 1,865 observations of reproductive outcomes for female spotted owls. The proportion of subadult females among all territorial females of known age ranged from 0.00 to 0.25 among study areas and years. The proportion of subadults among female spotted owls was negatively related to reproductive output (no. of young fledged/territorial F owl) for ELD and SIE. Eldorado study area and LAS showed an alternate-year trend in reproductive output, with higher output in even-numbered years. Mean annual reproductive output was 0.988 ?? 0.154 for ELD, 0.624 ?? 0.140 for LAS, 0.478 ?? 0.106 for SIE, and 0.555 ?? 0.110 for SKC. Eldorado Study Area exhibited a declining trend and the greatest variation in reproductive output over time, whereas SIE and SKC, which had the lowest reproductive output, had the lowest temporal variation. Meta-analysis confirmed that reproductive output varied among study areas. Reproductive output was highest for adults, followed by second-year subadults, and then by first-year subadults. We used 842 marked subadult and adult owls to estimate population rate of change. Modeling indicated that ??t (??t is the finite rate of population change estimated using the reparameterized JollySeber estimator Pradel 1996) was either stationary (LAS and SIE) or increasing after an initial decrease (ELD and SKC). Mean estimated ??t for the 4 study areas was 1.007 (95 CI 0.9521.066) for ELD; 0.973 (95 CI 0.9461.001) for LAS; 0.992 (95 CI 0.9661.018) for SIE; and 1.006 (95 CI 0.9471.068) for SKC. The best meta-analysis model of population trend indicated that ?? varied across time but was similar in trend among the study areas. Our estimates of realized population change (??t; Franklin et al. 2004), which we estimated as the product 1 ?? ??3 ?? ??4 ?? .?? ??k -1, were based on estimates of ??t from individual study areas and did not require estimating annual population size for each study area. Trends represented the proportion of the population size in the first ye

Wildlife Monographs↗

Chemical contaminants, health indicators, and reproductive biomarker responses in fish from the Colorado River and its tributaries

Common carp (Cyprinus carpio), black bass (Micropterus spp.), and channel catfish (Ictalurus punctatus) were collected from 14 sites in the Colorado River Basin (CRB) to document spatial trends in accumulative contaminants, health indicators, and reproductive biomarkers. Organochlorine residues, 2,3,7,8-tetrachlorodibenzo-p-dioxin-like activity (TCDD-EQ), and elemental contaminants were measured in composite samples of whole fish, grouped by species and gender, from each site. Selenium (Se) and mercury (Hg) concentrations in fish were elevated throughout the CRB, and pesticide concentrations were greatest in fish from agricultural areas in the Lower Colorado River and Gila River. Selenium concentrations exceeded toxicity thresholds for fish (> 1.0????g/g ww) at all CRB sites except the Gila River at Hayden, Arizona. Mercury concentrations were elevated (> 0.1????g/g ww) in fish from the Yampa River at Lay, Colorado; the Green River at Ouray National Wildlife Refuge (NWR), Utah and San Rafael, Utah; the San Juan River at Hogback Diversion, New Mexico; and the Colorado River at Gold Bar Canyon, Utah, Needles, California, and Imperial Dam, Arizona. Concentrations of p,p???-DDE were relatively high in fish from the Gila River at Arlington, Arizona (> 1.0????g/g ww) and Phoenix, Arizona (> 0.5????g/g ww). Concentrations of other formerly used pesticides including toxaphene, total chlordanes, and dieldrin were also greatest at these two sites but did not exceed toxicity thresholds. Currently used pesticides such as Dacthal, endosulfan, ??-HCH, and methoxychlor were also greatest in fish from the Gila River downstream of Phoenix. Total polychlorinated biphenyls (PCBs; > 0.11????g/g ww) and TCDD-EQs (> 5??pg/g ww) exceeded wildlife guidelines in fish from the Gila River at Phoenix. Hepatic ethoxyresorufin O-deethylase (EROD) activity was also relatively high in carp from the Gila River at Phoenix and in bass from the Green River at Ouray NWR. Fish from some sites showed evidence of contaminant exposure as indicated by fish health indicators and reproductive biomarker results. Multiple health indicators including altered body and organ weights and high health assessment index scores may be associated with elevated Se concentrations in fish from the Colorado River at Loma, Colorado and Needles. Although grossly visible external or internal lesions were found on most fish from some sites, histopathological analysis determined many of these to be inflammatory responses associated with parasites. Edema, exophthalmos, and cataracts were noted in fish from sites with elevated Se concentrations. Intersex fish were found at seven of 14 sites and included smallmouth bass (M. dolomieu), largemouth bass (M. salmoides), catfish, and carp and may indicate exposure to endocrine disrupting compounds. A high proportion of smallmouth bass from the Yampa River at Lay (70%) was intersex but the cause of this condition is unknown. Male carp, bass, and catfish with low concentrations of vitellogenin were common in the CRB. Comparatively high vitellogenin concentrations (> 0.2??mg/mL) were measured in male bass from the Green River at Ouray NWR and the Colorado River at Imperial Dam and indicate exposure to estrogenic or anti-androgenic chemicals. Anomalous reproductive biomarkers including low GSI and gonadal abnormalities (calcifications, edema, and parasites) observed in fish downstream of Phoenix are likely related to the poor water-quality of the Gila River in this area.

Colorado River Basin↗

Effects of tidally varying river flow on entrainment of juvenile salmon into Sutter and Steamboat Sloughs

Survival of juvenile salmonids in the Sacramento–San Joaquin Delta (Delta) varies by migration route, and thus the proportion of fish that use each route affects overall survival through the Delta. Understanding factors that drive routing at channel junctions along the Sacramento River is therefore critical to devising management strategies that maximize survival. Here, we examine entrainment of acoustically tagged juvenile Chinook Salmon into Sutter and Steamboat sloughs from the Sacramento River. Because these sloughs divert fish away from the downstream entrances of the Delta Cross Channel and Georgiana Slough (where fish access the low-survival region of the interior Delta), management actions to increase fish entrainment into Sutter and Steamboat sloughs are being investigated to increase through-Delta survival. Previous studies suggest that fish generally “go with the flow”—as net flow into a divergence increases, the proportion of fish that enter that divergence correspondingly increases. However, complex tidal hydrodynamics at sub-daily time-scales may be decoupled from net flow. Therefore, we modeled routing of acoustic tagged juvenile salmon as a function of tidally varying hydrodynamic data, which was collected using temporary gaging stations deployed between March and May of 2014. Our results indicate that discharge, the proportion of flow that entered the slough, and the rate of change of flow were good predictors of an individual’s probability of being entrained. In addition, interactions between discharge and the proportion of flow revealed a non-linear relationship between flow and entrainment probability. We found that the highest proportions of fish are likely to be entrained into Steamboat Slough and Sutter Slough on the ascending and descending limbs of the tidal cycle, when flow changes from positive to negative. Our findings characterize how patterns of entrainment vary with tidal flow fluctuations, providing information critical for understanding the potential effect of management actions (e.g., fish guidance structures) to modify routing probabilities at this location. Main Content View Larger 1 Sponsored by the Delta Science Program and the UC Davis Muir Institute ABSTRACT Survival of juvenile salmonids in the Sacramento–San Joaquin Delta (Delta) varies by migration route, and thus the proportion of fish that use each route affects overall survival through the Delta. Understanding factors that drive routing at channel junctions along the Sacramento River is therefore critical to devising management strategies that maximize survival. Here, we examine entrainment of acoustically tagged juvenile Chinook Salmon into Sutter and Steamboat sloughs from the Sacramento River. Because these sloughs divert fish away from the downstream entrances of the Delta Cross Channel and Georgiana Slough (where fish access SFEWS Volume 19 | Issue 2 | Article 4 https://doi.org/10.15447/sfews.2021v19iss2art4 * Corresponding author: rperry@usgs.gov 1 Western Fisheries Research Center US Geological Survey Cook, WA 98605 USA 2 California Water Science Center US Geological Survey Sacramento, CA 95819 USA 3 Current address: Mid-Columbia Fish and Wildlife Conservation Office Yakima Basin Program US Fish and Wildlife Service Yakima, WA 98903 USA the low-survival region of the interior Delta), management actions to increase fish entrainment into Sutter and Steamboat sloughs are being investigated to increase through-Delta survival. Previous studies suggest that fish generally “go with the flow”—as net flow into a divergence increases, the proportion of fish that enter that divergence correspondingly increases. However, complex tidal hydrodynamics at sub-daily time-scales may be decoupled from net flow. Therefore, we modeled routing of acoustic tagged juvenile salmon as a function of tidally varying hydrodynamic data, which was collected using temporary gaging stations deployed between March and May of 2014. Our results indicate that discharge, the proportion of flow that entered the slough, and the rate of change of flow were good predictors of an individual’s probability of being entrained. In addition, interactions between discharge and the proportion of flow revealed a non-linear relationship between flow and entrainment probability. We found that the highest proportions of fish are likely to be entrained into Steamboat Slough and Sutter Slough on the ascending and descending limbs of the tidal cycle, when flow changes from positive to negative. Our findings characterize how patterns of entrainment vary with tidal flow fluctuations, providing information critical for understanding the potential effect of management RESEARCH Effects of Tidally Varying River Flow on Entrainment of Juvenile Salmon into Sutter and Steamboat Sloughs Jason G. Romine 1,3 , Russell W. Perry* 1 , Paul R. Stumpner 2 , Aaron R. Blake 2 , Jon R. Burau 2 2 VOLUME 19, ISSUE 2, ARTICLE 4 actions (e.g., fish guidance structures) to modify routing probabilities at this location. KEY WORDS Telemetry, juvenile salmon, migration routing, survival INTRODUCTION The Sacramento–San Joaquin River Delta (hereafter referred to as “the Delta”) is a complex series of channels and embayments in west central California of the United States. The Delta has undergone drastic transformation through construction of dikes, levees, reclaimed land, dredged canals and cuts, and water export projects (Nichols et al. 1986). The loss of habitat coupled with introduction of non-native piscivorous fishes has led to the decline of several salmonid stocks that utilize the Delta (Lindley 2009; National Marine Fisheries Service 2014). The physical complexity of the Delta poses significant challenges for understanding how juvenile salmon negotiate the complex channel network and survive in different migration routes. Yet such information is critical for understanding how water-management actions, such as operation of water diversions, influence survival of juvenile salmon. Through-Delta survival of juvenile Chinook Salmon that emigrate from the Sacramento River ranges from 10% to 80%, depending on river flow and migration route (Perry et al. 2018). The Delta can be broken down into four primary routes: (1) Sacramento River, (2) Steamboat and Sutter sloughs, (3) Georgiana Slough, and (4) Delta Cross Channel (DCC). Fish that remain in the Sacramento River consistently have the highest survival (Perry et al. 2010, 2013, 2018). However, fish that enter the interior Delta—the region to the south of the Sacramento River (Figure 1)—have the lowest survival among all routes and survive at less than half the rate of fish in the Sacramento River, likely as a result of longer migration times and exposure to non-native predators (Newman and Brandes 2010; Perry et al. 2018). On average, fish that migrate through Steamboat and Sutter sloughs exhibit survival similar to fish that remain in the Sacramento River at high flows but have lower survival at low flows (Perry et al. 2018). Because of differences in survival among migration routes, the proportion of fish that use each route affects the total survival of the population. Therefore, understanding the drivers behind fish routing in the Delta is imperative to inform management actions that help in the recovery of imperiled salmonid populations in the Central Valley. For example, Perry et al. (2013) found that total survival through the Delta could be increased by up to 7 percentage points by eliminating entrainment into Georgiana Slough and the DCC. These findings led to investigation of management actions to reduce entrainment into the DCC (Plumb et al. 2016) and Georgiana Slough (Perry et al. 2014). Both physical and non-physical barriers have been tested at the entrance to Georgiana Slough divergence (Perry et al. 2014; Romine et al. 2016). A non-physical barrier was able to reduce entrainment to the interior Delta through Georgiana Slough (Perry et al. 2014), but a floating fish-guidance structure reduced entrainment to a lesser extent (Romine et al. 2016). Research and engineering solutions to minimize entrainment have focused on the Georgiana Slough divergence, the DCC divergence, and the Old River divergence in the San Joaquin River (Buchanan et al. 2013; SJRG 2013). However, there has been little focus on understanding fish routing dynamics at other primary river junctions in the Delta, such as Sutter and Steamboat sloughs. Sutter and Steamboat sloughs diverge from the Sacramento about 10 km upstream from the DCC and Georgiana slough, and represent the first major junction that juvenile salmon encounter as they enter the Delta from the Sacramento River ( Figure 1 ). Because Sutter and Steamboat sloughs are upstream of the entrance to the interior Delta via the DCC and Georgiana Slough (Figure 1), juvenile salmon that enter Sutter and Steamboat sloughs avoid entrainment into the interior Delta where survival is low. Thus, management actions to increase entrainment could increase overall.

Sacramento–San Joaquin Delta↗

Regression models for explaining and predicting concentrations of organochlorine pesticides in fish from streams in the United States

Empirical regression models were developed for estimating concentrations of dieldrin, total chlordane, and total DDT in whole fish from U.S. streams. Models were based on pesticide concentrations measured in whole fish at 648 stream sites nationwide (1992-2001) as part of the U.S. Geological Survey's National Water Quality Assessment Program. Explanatory variables included fish lipid content, estimates (or surrogates) representing historical agricultural and urban sources, watershed characteristics, and geographic location. Models were developed using Tobit regression methods appropriate for data with censoring. Typically, the models explain approximately 50 to 70% of the variability in pesticide concentrations measured in whole fish. The models were used to predict pesticide concentrations in whole fish for streams nationwide using the U.S. Environmental Protection Agency's River Reach File 1 and to estimate the probability that whole-fish concentrations exceed benchmarks for protection of fish-eating wildlife. Predicted concentrations were highest for dieldrin in the Corn Belt, Texas, and scattered urban areas; for total chlordane in the Corn Belt, Texas, the Southeast, and urbanized Northeast; and for total DDT in the Southeast, Texas, California, and urban areas nationwide. The probability of exceeding wildlife benchmarks for dieldrin and chlordane was predicted to be low for most U.S. streams. The probability of exceeding wildlife benchmarks for total DDT is higher but varies depending on the fish taxon and on the benchmark used. Because the models in the present study are based on fish data collected during the 1990s and organochlorine pesticide residues in the environment continue to decline decades after their uses were discontinued, these models may overestimate present-day pesticide concentrations in fish. ?? 2009 SETAC.

Environmental Toxicology and Chemistry↗

Transformation through time: How wildlife disease became a focus of conservation

When I began my career as an assistant waterfowl biologist in 1956, wildlife disease was not a major concern for conservation agencies. Some states— such as California, Michigan, New York, Wyoming, and Colorado— had small internal wildlife disease programs to investigate wildlife mortality events, and the U.S. Fish and Wildlife Service (FWS) had a program focused on migratory birds.

The Wildlife Professional↗