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Genotyping-by-sequencing illuminates high levels of divergence among sympatric forms of coregonines in the Laurentian Great Lakes

Effective resource management depends on our ability to partition diversity into biologically meaningful units. Recent evolutionary divergence, however, can often lead to ambiguity in morphological and genetic differentiation, complicating the delineation of valid conservation units. Such is the case with the "coregonine problem," where recent postglacial radiations of coregonines into lacustrine habitats resulted in the evolution of numerous species flocks, often with ambiguous taxonomy. The application of genomics methods is beginning to shed light on this problem and the evolutionary mechanisms underlying divergence in these ecologically and economically important fishes. Here, we used restriction site-associated DNA (RAD) sequencing to examine genetic diversity and differentiation among sympatric forms in the Coregonus artedi complex in the Apostle Islands of Lake Superior, the largest lake in the Laurentian Great Lakes. Using 29,068 SNPs, we were able to clearly distinguish among the three most common forms for the first time, as well as identify putative hybrids and potentially misidentified specimens. Population assignment rates for these forms using our RAD data were 93%-100% with the only mis-assignments arising from putative hybrids, an improvement from 62% to 77% using microsatellites. Estimates of pairwise differentiation ( F ST : 0.045-0.056) were large given the detection of hybrids, suggesting that reduced fitness of hybrid individuals may be a potential mechanism for the maintenance of differentiation. We also used a newly built C. artedi linkage map to look for islands of genetic divergence among forms and found widespread differentiation across the genome, a pattern indicative of long-term drift, suggesting that these forms have been reproductively isolated for a substantial amount of time. The results of this study provide valuable information that can be applied to develop well-informed management strategies and stress the importance of re-evaluating conservation units with genomic tools to ensure they accurately reflect species diversity.

Wisconsin↗

Assessment of soil-gas contamination at building 310 underground storage tank area, Fort Gordon, Georgia, 2010-2011

Soil gas was assessed for contaminants in the building 310 underground storage tank area adjacent to the Dwight D. Eisenhower Army Medical Center at Ft. Gordon, Georgia, from October 2010 to September 2011. The assessment, which also included the detection of organic compounds in soil gas, provides environmental contamination data to Fort Gordon personnel pursuant to requirements of the Resource Conservation and Recovery Act Part B Hazardous Waste Permit process. The study was conducted by the U.S. Geological Survey, in cooperation with the U.S. Department of the Army Environmental and Natural Resources Management Office of the U.S. Army Signal Center and Fort Gordon. Soil-gas samplers were deployed below land surface at 37 locations in the building 310 underground storage tank area. Soil-gas samplers were deployed in a grid pattern near the storage tank area as well as downslope of the tank area in the direction of groundwater flow toward an unnamed tributary to Butler Creek. Total petroleum hydrocarbons were detected in 35 of the 37 soil-gas samplers at levels above the method detection level, and the combined mass of benzene, toluene, ethylbenzene, and total xylenes were detected above their detection levels in 8 of the 37 samplers. In addition, the combined masses of undecane, tridecane, and pentadecane were detected at or above their method detection levels in 9 of the 37 samplers. Other volatile organic compounds detected above their respective method detection levels were chloroform, 1,2,4-trimethylbenzene, and perchloroethylene. In addition, naphthalene, 2-methyl naphthalene, and 1,2,4-trimethylbenzene were detected below the method detection levels, but above the nondetection level.

Georgia↗

Alaska index (December 1980 - November 1981)

In an effort to develop the oil and gas resources of the Outer Continental Shelf (OCS), the United States currently conducts a leasing program for these resources. The Secretary of the Department of the Interior (DOI) has been designated by the Outer Continental Shelf Lands Act of 1953 (43 U.S.C. 1331-1343), as amended by the Outer Continental Shelf Lands Act Amendments of 1978 (43 U.S.C. 1801-1866), to manage and regulate many of the activities that relate to the leasing, exploration, development, and production of the OCS mineral resources. Many of the Secretary's responsibilities have been delegated to the Bureau of Land Management (BLM) and the Minerals Management Service (MMS). Responsibilities of the Minerals Management Service were formerly delegated to the Conservation Division of the U.S. Geological Survey (USGS).

Alaska↗

Delineation of areas contributing groundwater and travel times to receiving waters in Kings, Queens, Nassau, and Suffolk Counties, New York

To assist resource managers and planners in developing informed strategies to address nitrogen loading to coastal water bodies of Long Island, New York, the U.S. Geological Survey and New York State Department of Environmental Conservation initiated a program to delineate areas contributing groundwater to coastal water bodies by assembling a comprehensive dataset of areas contributing groundwater, travel times, and groundwater discharges to streams, lakes, marine surface waters, and subsea discharge boundaries. Steady-state, 25-layer regional, three-dimensional finite-difference groundwater-flow models of average regional hydrologic conditions were used for particle-tracking analysis to delineate areas contributing groundwater to 843 water bodies. Two steady-state conditions were simulated: recent conditions from 2005 to 2015 and predevelopment conditions of about 1900. About 14 million particles were evenly distributed across the water table and tracked forward to discharge zones. Using a uniform porosity of 25 percent, simulated recent condition travel times ranged from less than 2 years to greater than 10,000 years and were visualized in 11 travel time intervals. About 85 percent of particle travel times from the water table to points of discharge are less than 100 years. Simulated particle-tracking ending zones represented 843 receiving water bodies, based on the New York State Department of Environmental Conservation water body inventory and priority water bodies list. Areal delineation of travel-time intervals and areas contributing groundwater to water bodies were generated and are summarized with total groundwater outflow for each water body.

New York↗

Discharge and dissolved-solids characteristics of Blacks Fork above Smiths Fork, Wyoming, April 2018 through September 2019

The Colorado River Basin Salinity Control Forum was formed in 1973 to coordinate salinity control efforts among the States in the Colorado River Basin, including Wyoming. The Colorado River Salinity Control Act of 1974 (Public Law 93–320) authorized “the construction, operation, and maintenance of certain works in the Colorado River Basin to control the salinity of water delivered to users in the United States and Mexico.” Water-quality standards for salinity in the lower Colorado River Basin were adopted in 1975. To help meet these standards, the Bureau of Reclamation, Natural Resource Conservation Service, and States within the Colorado River Basin have implemented salinity control projects that focus on reducing salt loading associated with irrigated agriculture by improving water delivery systems and water management practices. The term salinity is synonymous with dissolved solids in this report. The Bureau of Reclamation, in conjunction with the Colorado River Basin Salinity Control Forum, was interested in determining the contribution of dissolved solids from Blacks Fork above Smiths Fork to the Colorado River and initiated a study of Blacks Fork above Smiths Fork in 2018. In early 2018, the U.S. Geological Survey installed a streamgage at the most downstream location on the Blacks Fork, upstream from the convergence with Smiths Fork, to characterize the stream. The Blacks Fork above Smiths Fork, near Lyman, Wyoming, streamgage (U.S. Geological Survey identifier 09219200) was operated from April 4, 2018, through September 30, 2019, collecting continuous stream stage and specific-conductance data, from which continuous discharge, dissolved-solids concentrations, and dissolved-solids loads were calculated. Seven sites were selected on Blacks Fork and a tributary to describe a snapshot of the discharge and dissolved-solids characteristics. These sites were sampled during July, August, and September 2018 and June, July, August, and September 2019 report. Discharge at the Blacks Fork above Smiths Fork, near Lyman, Wyo., streamgage (09219200) from April through September in 2018 was lower and less variable than during the same period in 2019. The mean daily (mean of the daily means) discharge during those 6 months in 2018 (15.1 cubic feet per second [ft 3 /s]) was about one-tenth of the discharge during the same period in 2019 (152 ft 3 /s). The cumulative monthly discharge during April through September in 2018 was 5,360 acre-feet, about one-tenth of the discharge during the same period in 2019 which was 54,700 acre-feet. Similar differences in discharge between the 2018 and 2019 periods also are noted at other Blacks Fork streamgages in the area. Continuous specific conductance data and the statistical relation between specific conductance and dissolved-solids concentrations were used to calculate the daily mean dissolved-solids concentrations. Dissolved solids often have an inverse relation with discharge because higher discharges typically have a diluting effect that lowers the dissolved-solids concentrations. In general, when discharges at the Blacks Fork above Smiths Fork streamgage (09219200) are higher, dissolved-solids concentrations are generally lower. However, the high dissolved-solids concentrations that are measured during high discharges indicate that the system has natural variability and the dissolved-solids concentrations are determined by more factors than just discharge. The mean daily dissolved-solids concentration during April through September 2018 was 1,630 milligrams per liter and during the same period in 2019 was 1,100 milligrams per liter. Dissolved-solids loads were calculated as the product of the discharge and dissolved-solids concentration. The daily mean dissolved-solids loads during 2018 were typically lower than during 2019. This result is primarily because the discharge was much lower in 2018 than in 2019. Therefore, although the daily mean dissolved-solids concentrations tended to be higher in 2018, the substantially higher discharges in 2019 had more of an effect on the dissolved-solids loads than the dissolved-solids concentrations. The cumulative dissolved-solids load at the Blacks Fork above Smiths Fork, near Lyman, Wyo., streamgage (09219200) during the 18-month study was 81,200 tons, with a mean daily load of 149 tons per day. During the 6-month period from April through September 2018, the cumulative dissolved-solids load at the streamgage was estimated to be 8,740 tons and, during the same 6 months in 2019, the cumulative dissolved-solids load was estimated to be 60,900 tons. During the fall and winter between the two periods, the cumulative dissolved-solids load was 11,600 tons. Discharge and dissolved-solids concentrations from samples collected during the synoptic sampling events were highly variable among most sites during most synoptic sampling events and also highly variable at most sites among different sampling events. The two sites upstream from the tributary input from Threemile Creek had lower dissolved-solids concentrations than sites including and downstream from the tributary. Sites including and downstream from the tributary had similar values and variability of dissolved-solids loads, with the exception of the farthest downstream site at the Blacks Fork above Smiths Fork, near Lyman, Wyo., streamgage (09219200) that tended to have larger dissolved-solids loads and higher variability among synoptic sampling events.

Scientific Investigations Report↗

Bottom trawl assessment of Lake Ontario prey fishes

Collaborative Lake Ontario bottom trawl surveys, led by the United States Geological Survey (USGS), provide science and management information for evaluating Fish Community Objectives including predator-prey balance and prey fish community diversity. In 2018, the New York State Department of Environmental Conservation (NYSDEC), Ontario Ministry of Natural Resources and Forestry (OMNR), and the (USGS) completed an April bottom trawl survey (n = 208 tows) and an October survey (n = 118 tows), at depths 6-228 m, and captured 384,651 fish from 31 species. Alewife were 80% of the total catch by number and round goby, deepwater sculpin, and rainbow smelt comprised 12, 4, and 3% of the catch, respectively. The adult alewife abundance index for U.S. waters decreased in 2018 relative to 2017, while the index in Canadian waters increased. While lake wide density increased, biomass indices for Age-2 alewife decreased. Alewife condition indices were below the 10-year average for both the April and October indices. The 2018 Age-1 alewife abundance index, which measures reproductive success the previous year, was the third lowest observed in U.S. waters over the past 22 years. The Canadian Age-1 index 2018 value was four-times larger than the U.S. value. Within-year differences between Canadian and U.S. alewife abundance indices, highlight the importance of assessing Lake Ontario fishes at a whole-lake scale. Abundance indices for rainbow smelt, threespine stickleback and emerald shiner were similar to 2017. New experimental trawl sites in embayment habitats generally captured more species, a higher proportion of native species, and higher densities relative to similar depth sites in the main lake and regions adjacent to embayments. Pelagic prey fish diversity continues to be low because a single species, alewife, dominates the catch. Deepwater sculpin and round goby were the most abundant demersal (bottom-oriented) prey fishes in 2018. Slimy sculpin and native nearshore demersal prey fishes, which were historically more abundant in trawl catches, are rare and restricted to specific habitats, since round goby proliferation. Despite declines in some species, demersal prey fish community diversity continues to increase as deepwater sculpin and round goby comprise more even portions of the community in contrast to when a single species, slimy sculpin, dominated the community. Five bloater were captured in the 2018 surveys which is the largest number captured in Lake Ontario since restoration stocking began in 2012.

Lake Ontario↗

Ecological influence and pathways of land use in sagebrush

Land use in sagebrush (Artemisia spp.) landscapes influences all sage-grouse (Centrocer-cus spp.) populations in western North America. Croplands and the network of irrigation canals cover 230,000 km2 and indirectly influence up to 77% of the Sage-Grouse Conservation Area and 73% of sagebrush land cover by subsidizing synanthropic predators on sage-grouse. Urbanization and the demands of human population growth have created an extensive network of con-necting infrastructure that is expanding its influence on sagebrush landscapes. Over 2,500 km 2 are now covered by interstate highways and paved roads; when secondary roads are included, 15% of the Sage-Grouse Conservation Area and 5% of existing sagebrush habitats are 2.5 km from roads. Density of secondary roads often exceeds 5 km/km 2 , resulting in widespread motorized access for recreation, creating extensive travel corridors for management actions and resource development, subsidizing predators adapted to human presence, and facilitating spread of exotic or invasive plants. Sagebrush lands also are being used for their wilderness and recreation values, including off highway vehicle use. Approximately 12,000,000 animal use months (AUM amount of forage to support one livestock unit per month) are permitted for grazing livestock on public lands in the western states. Direct effects of grazing on sage-grouse populations or sagebrush landscapes are not possible to assess from current data. However, management of lands grazed by livestock has influenced sagebrush ecosystems by vegetation treatments to increase forage and reduce sagebrush and other plant species unpalatable to livestock. Fences (2 km/km 2 in some regions), roads, and water developments to manage livestock movements further modify the landscape. Oil and gas development influences 8% of the sagebrush habitats with the highest intensities occurring in the eastern range of sage-grouse; 20% of the sagebrush distribution is indirectly influenced in the Great Plains, Wyoming Basin, and Colorado Plateau SMZs. Energy development physically removes habitat to construct well pads, roads, power lines, and pipelines; indirect effects include habitat fragmentation, soil disturbance, and facilitation of exotic plant and animal spread. More recent development of alternative energy, such as wind and geothermal, creates infrastructure in new regions of the sage-grouse distribution. Land use will continue to be a dominant stressor on sage-brush systems; its individual and cumulative effects will challenge long-term conservation of sage-grouse populations.

Book chapter↗

Ten ways remote sensing can contribute to conservation

In an effort to increase conservation effectiveness through the use of Earth observation technologies, a group of remote sensing scientists affiliated with government and academic institutions and conservation organizations identified 10 questions in conservation for which the potential to be answered would be greatly increased by use of remotely sensed data and analyses of those data. Our goals were to increase conservation practitioners’ use of remote sensing to support their work, increase collaboration between the conservation science and remote sensing communities, identify and develop new and innovative uses of remote sensing for advancing conservation science, provide guidance to space agencies on how future satellite missions can support conservation science, and generate support from the public and private sector in the use of remote sensing data to address the 10 conservation questions. We identified a broad initial list of questions on the basis of an email chain-referral survey. We then used a workshop-based iterative and collaborative approach to whittle the list down to these final questions (which represent 10 major themes in conservation): How can global Earth observation data be used to model species distributions and abundances? How can remote sensing improve the understanding of animal movements? How can remotely sensed ecosystem variables be used to understand, monitor, and predict ecosystem response and resilience to multiple stressors? How can remote sensing be used to monitor the effects of climate on ecosystems? How can near real-time ecosystem monitoring catalyze threat reduction, governance and regulation compliance, and resource management decisions? How can remote sensing inform configuration of protected area networks at spatial extents relevant to populations of target species and ecosystem services? How can remote sensing-derived products be used to value and monitor changes in ecosystem services? How can remote sensing be used to monitor and evaluate the effectiveness of conservation efforts? How does the expansion and intensification of agriculture and aquaculture alter ecosystems and the services they provide? How can remote sensing be used to determine the degree to which ecosystems are being disturbed or degraded and the effects of these changes on species and ecosystem functions?

Conservation Biology↗

Using high resolution satellite and telemetry data to track flooded habitats, their use by waterfowl, and evaluate effects of drought on waterfowl and shorebird bioenergetics in California

Wetland managers in the Central Valley of California, a dynamic hydrological landscape, require information regarding the amount and location of existing wetland habitat to make decisions on how to best use water resources to support multiple wildlife objectives, particularly during drought. Scientists from the U.S. Geological Survey Western Ecological Research Center (WERC), Point Blue Conservation Science (Point Blue), and the U.S. Fish and Wildlife Service (USFWS) partnered to learn how wetland and flooded agricultural habitats used by waterfowl and shorebirds change during the non-breeding season (July–April) particularly during drought. During extreme drought conditions, the ability to provide sufficient water for wildlife often depends on the timing of water deliveries to managed wetlands and winter-flooded crop fields and decisions on whether to fallow croplands. Waterfowl and shorebirds could be particularly affected by these decisions because they typically rest and feed in flooded habitats. Poor habitat conditions resulting from spatially or temporally suboptimal water deliveries (that is, mismatch between waterfowl habitat needs and timing and location of flooded habitats) could reduce waterfowl hunting opportunities and body condition. Point Blue scientists developed a system for near real-time tracking of habitats used by waterfowl, shorebirds, and some other wetland-dependent “waterbirds” ( www.pointblue.org/watertracker ) and to assess the impacts of drought on habitat availability and on waterfowl and shorebird bioenergetics. The WERC researchers linked these data with near real-time tracking (telemetry) data of duck locations throughout the Valley. The team used these two datasets to relate duck locations to open-water characteristics and to learn how waterfowl use habitats under spatially and temporally changing conditions during drought and non-drought periods. We found that recent extreme drought (2013–15) significantly changed the timing and magnitude of flooding and consequently reduced the availability of habitats used by waterfowl and shorebirds more than other recent historic droughts 2000–11. Drought reduced irrigations of moist soil seed plants and thus there was lower food energy available for waterfowl. Analyses using bioenergetics models indicated that, overall, extreme drought increased food energy deficits (total number of deficit days) for shorebirds and waterfowl. Our analysis indicated a strong direct relationship between duck locations and classified habitat derived from open-water data during the wintering period (October–March). This result helps confirm the application of dynamic water data to identify flooded areas that provide waterfowl habitat. Presence of open water at a 1-hectare resolution can be used effectively to identify flooded landscape areas available as habitat for ducks. Our discoveries from evaluating use of space by ducks also indicated that nighttime feeding locations of ducks were concentrated nearby primary roosts and that foraging distances could depend on hydrologic dynamics of location (Suisun Marsh versus California excluding Suisun Marsh) and time of season (early, middle, late). Other results indicated that some areas on the California landscape with extremely reliable water supplies could receive consistent use by ducks year after year (in essence, almost drought proof). The Water Tracker is set up to automatically track wetland habitat and food availability each year and is making these data available to water and wetland managers. Results from this research are a significant step toward understanding how waterfowl and shorebird habitats can be optimally managed on the landscape to support desired populations of these migratory birds during extreme drought.

California↗

Applying threshold concepts to conservation management of dryland ecosystems: Case studies on the Colorado Plateau

Ecosystems may occupy functionally distinct alternative states, some of which are more or less desirable from a management standpoint. Transitions from state to state are usually associated with a particular trigger or sequence of triggers, such as the addition or subtraction of a disturbance. Transitions are often not linear, rather it is common to see an abrupt transition come about even though the trigger increases only incrementally; these are examples of threshold behaviors. An ideal monitoring program, such as the National Park Service’s Inventory and Monitoring Program, would quantify triggers, and be able to inform managers when measurements of a trigger are approaching a threshold so that management action can avoid an unwanted state transition. Unfortunately, both triggers and the threshold points at which state transitions occur are generally only partially known. Using case studies, we advance a general procedure to help identify triggers and estimate where threshold dynamics may occur. Our procedure is as follows: (1) Operationally define the ecosystem type being considered; we suggest that the ecological site concept of the Natural Resource Conservation Service is a useful system, (2) Using all available a priori knowledge to develop a state-and-transition model (STM), which defines possible ecosystem states, plausible transitions among them and likely triggers, (3) Validate the STM by verifying the existence of its states to the greatest degree possible, (4) Use the STM model to identify transitions and triggers likely to be detectable by a monitoring program, and estimate to the greatest degree possible the value of a measurable indicator of a trigger at the point that a state transition is imminent (tipping point), and values that may indicate when management intervention should be considered (assessment points). We illustrate two different methods for attaining these goals using a data-rich case study in Canyonlands National Park, and a data-poor case study in Wupatki National Monument. In the data-rich case, STMs are validated and revised, and tipping and assessment points are estimated using statistical analysis of data. In the data-poor case, we develop an iterative expert opinion survey approach to validate the degree of confidence in an STM, revise the model, identify lack of confidence in specific model components, and create reasonable first approximations of tipping and assessment points, which can later be refined when more data are available. Our goal should be to develop the best set of models possible given the level of information available to support decisions, which is often not much. The approach presented here offers a flexible means of achieving this goal, and determining specific research areas in need of study.

Book chapter↗

Managing water in the West: developing new tools for a critical resource

Rapid population growth in the Western United States over the last century has placed increasing strains on our water supplies and aquatic ecosystems. Historically, water rights have been used to determine the allocation of water in the West, but rules and regulations related to endangered species now often drive how water is released from reservoirs in large rivers such as the lower Colorado and the Columbia. In numerous smaller watersheds, communities are trying to balance the water necessary for human use, irrigation, and the conservation of ecosystems. To assist managers in the face of increasing complexity and uncertainty in water management decision-making, the Western Fisheries Research Center (WFRC) is involved in developing a new generation of integrative tools. Below are some examples of the types of tools that already exist within the WFRC.

Fact Sheet↗

Conservation of marine birds of northern North America: Papers from the international symposium held at the Seattle Hyatt House, Seattle, Washington, 13-15 May 1975

The international symposium "Conservation of Marine Birds of Northern North America" was convened because of a growing awareness that not all was well with our marine birds. The symposium provided a forum for scientists, governmental administrators, conservationists, and laypeople to discuss the diverse topics and issues that we must all understand if we are to act both responsively and responsibly to assure that marine birds will not be lost through our neglect. The symposium was cosponsored by the Natural Resources Council of America, National Audubon Society, National Wildlife Federation, and the U.S. Department of the Interior, Fish and Wildlife Service; additional support was provided by the Canadian Wildlife Service, the International Association of Game, Fish, and Conservation Commissioners, the Pacific Seabird Group, the Sierra Club, the Smithsonian Institution, the Wildlife Management Institute, and the Wildlife Society. Persons interested and knowledgeable in the many and varied aspects of marine bird conservation were invited to participate in this symposium. There were 139 registered and several score of unregistered participants in attendance. Major topics treated were: (1) socioeconomic considerations and conservation of marine birds; (2) the marine environment of birds; (3) status of marine bird populations on land and sea; (4) the biology and ecology of marine birds in the North; (5) conflicts between the conservation of marine birds and uses of other resources; (6) programs and authorities related to the conservation of marine birds; and (7) conservation of marine birds in other lands.

Wildlife Research Report↗

The challenges associated with developing science-based landscape scale management plans

Planning activities over large landscapes poses a complex of challenges when trying to balance the implementation of a conservation strategy while still allowing for a variety of consumptive and nonconsumptive uses. We examine a case in southeast Alaska to illustrate the breadth of these challenges and an approach to developing a science-based resource plan. Not only was the planning area, the Tongass National Forest, USA, exceptionally large (approximately 17 million acres or 6.9 million ha), but it also is primarily an island archipelago environment. The water system surrounding and going through much of the forest provides access to facilitate the movement of people, animals, and plants but at the same time functions as a barrier to others. This largest temperate rainforest in the world is an exceptional example of the complexity of managing at such a scale but also illustrates the role of science in the planning process. As we enter the 21st century, the list of questions needing scientific investigation has not only changed dramatically, but the character of the questions also has changed. Questions are contentious, cover broad scales in space and time, and are highly complex and interdependent. The provision of unbiased and objective information to all stakeholders is an important step in informed decision-making.

Landscape and Urban Planning↗

Ecoregions of California

Ecoregions denote areas of general similarity in ecosystems and in the type, quality, and quantity of environmental resources. They are designed to serve as a spatial framework for the research, assessment, management, and monitoring of ecosystems and ecosystem components. By recognizing the spatial differences in the capacities and potentials of ecosystems, ecoregions stratify the environment by its probable response to disturbance (Bryce and others, 1999). These general purpose regions are critical for structuring and implementing ecosystem management strategies across Federal agencies, State agencies, and nongovernment organizations that are responsible for different types of resources in the same geographical areas (Omernik and others, 2000). The approach used to compile this map is based on the premise that ecological regions are hierarchical and can be identified through the analysis of the spatial patterns and the composition of biotic and abiotic phenomena that affect or reflect differences in ecosystem quality and integrity (Wiken, 1986; Omernik, 1987, 1995). These phenomena include geology, physiography, vegetation, climate, soils, land use, wildlife, and hydrology. The relative importance of each characteristic varies from one ecological region to another regardless of the hierarchical level. A Roman numeral hierarchical scheme has been adopted for different levels of ecological regions. Level I is the coarsest level, dividing North America into 15 ecological regions. Level II divides the continent into 50 regions (Commission for Environmental Cooperation Working Group, 1997, map revised 2006). At level III, the continental United States contains 105 ecoregions and the conterminous United States has 85 ecoregions (U.S. Environmental Protection Agency, 2013). Level IV, depicted here for California, is a further refinement of level III ecoregions. Explanations of the methods used to define these ecoregions are given in Omernik (1995), Omernik and others (2000), and Omernik and Griffith (2014). California has great ecological and biological diversity. The State contains offshore islands and coastal lowlands, large alluvial valleys, forested mountain ranges, deserts, and various aquatic habitats. There are 13 level III ecoregions and 177 level IV ecoregions in California and most continue into ecologically similar parts of adjacent States of the United States or Mexico (Bryce and others, 2003; Thorson and others, 2003; Griffith and others, 2014). The California ecoregion map was compiled at a scale of 1:250,000. It revises and subdivides an earlier national ecoregion map that was originally compiled at a smaller scale (Omernik, 1987; U.S. Environmental Protection Agency, 2013). This poster is the result of a collaborative project primarily between U.S. Environmental Protection Agency (USEPA) Region IX, USEPA National Health and Environmental Effects Research Laboratory (Corvallis, Oregon), California Department of Fish and Wildlife (DFW), U.S. Department of Agriculture (USDA)–Natural Resources Conservation Service (NRCS), U.S. Department of the Interior–Geological Survey (USGS), and other State of California agencies and universities. The project is associated with interagency efforts to develop a common framework of ecological regions (McMahon and others, 2001). Reaching that objective requires recognition of the differences in the conceptual approaches and mapping methodologies applied to develop the most common ecoregion-type frameworks, including those developed by the USDA–Forest Service (Bailey and others, 1994; Miles and Goudy, 1997; Cleland and others, 2007), the USEPA (Omernik 1987, 1995), and the NRCS (U.S. Department of Agriculture–Soil Conservation Service, 1981; U.S. Department of Agriculture–Natural Resources Conservation Service, 2006). As each of these frameworks is further refined, their differences are becoming less discernible. Regional collaborative projects such as this one in California, where some agreement has been reached among multiple resource-management agencies, are a step toward attaining consensus and consistency in ecoregion frameworks for the entire nation.

California↗

Source-sink dynamics and the coexistence of species on a single resource

We investigate the potential for coexistence of species that compete for a shared resource when the resource occurs in both a source area acting as a refuge and a sink area where it is used by the competing species. Our model shows that the mixing rate between the source and sink resource populations has a dramatic influence on the outcome of competition. When there is a strict sink–source dependence, so that resource renewal in the sink is entirely dependent on the source, only exploitation competition decides which species can survive at a very small mixing rate. Increasing the flow rate basically amounts to increasing the role played by interference competition. If interspecific interference is very small, compared with intraspecific interference, increasing the mixing rate allows coexistence of many species, indeed, unlimited coexistence if the species' resource exploitation efficiencies are similar enough. If interspecific interference is significant and there is a trade-off between the exploitation and interference competitive abilities of two species, it is possible to have one species replacing the other along a gradient of increasing mixing rate, with either coexistence or alternative single-species equilibria at intermediate values of the mixing rate. It is also possible to have one species always outcompeting the other, or alternative single-species stable equilibria at large mixing rates. When the strict sink–source dependence is relaxed by allowing the resource to have a partly independent renewal–loss dynamics in the sink area, the variety of possible outcomes along a gradient of mixing rate is further increased. These outcomes are often strongly sensitive to the parameters of resource dynamics and interspecific interference competition. The implications of these results for biological conservation can be profound; detailed knowledge of interspecific interactions appears to be necessary to determine the effect of any land management that alters dispersal opportunities.

Theoretical Population Biology↗

Supplemental feeding alters migration of a temperate ungulate

Conservation of migration requires information on behavior and environmental determinants. The spatial distribution of forage resources, which migration exploits, often are altered and may have subtle, unintended consequences. Supplemental feeding is a common management practice, particularly for ungulates in North America and Europe, and carryover effects on behavior of this anthropogenic manipulation of forage are expected in theory, but have received limited empirical evaluation, particularly regarding effects on migration. We used global positioning system (GPS) data to evaluate the influence of winter feeding on migration behavior of 219 adult female elk (Cervus elaphus) from 18 fed ranges and 4 unfed ranges in western Wyoming. Principal component analysis revealed that the migratory behavior of fed and unfed elk differed in distance migrated, and the timing of arrival to, duration on, and departure from summer range. Fed elk migrated 19.2 km less, spent 11 more days on stopover sites, arrived to summer range 5 days later, resided on summer range 26 fewer days, and departed in the autumn 10 days earlier than unfed elk. Time-to-event models indicated that differences in migratory behavior between fed and unfed elk were caused by altered sensitivity to the environmental drivers of migration. In spring, unfed elk migrated following plant green-up closely, whereas fed elk departed the feedground but lingered on transitional range, thereby delaying their arrival to summer range. In autumn, fed elk were more responsive to low temperatures and precipitation events, causing earlier departure from summer range than unfed elk. Overall, supplemental feeding disconnected migration by fed elk from spring green-up and decreased time spent on summer range, thereby reducing access to quality forage. Our findings suggest that ungulate migration can be substantially altered by changes to the spatial distribution of resources, including those of anthropogenic origin, and that management practices applied in one season may have unintended behavioral consequences in subsequent seasons.

Wyoming↗

Mating behavior as a possible cause of bat fatalities at wind turbines

Bats are killed by wind turbines in North America and Europe in large numbers, yet a satisfactory explanation for this phenomenon remains elusive. Most bat fatalities at turbines thus far occur during late summer and autumn and involve species that roost in trees. In this commentary I draw on existing literature to illustrate how previous behavioral observations of the affected species might help explain these fatalities. I hypothesize that tree bats collide with turbines while engaging in mating behaviors that center on the tallest trees in a landscape, and that such behaviors stem from 2 different mating systems (resource defense polygyny and lekking). Bats use vision to move across landscapes and might react to the visual stimulus of turbines as they do to tall trees. This scenario has serious conservation and management implications. If mating bats are drawn to turbines, wind energy facilities may act as population sinks and risk may be hard to assess before turbines are built. Researchers could observe bat behavior and experimentally manipulate trees, turbines, or other tall structures to test the hypothesis that tree bats mate at the tallest trees. If this hypothesis is supported, management actions aimed at decreasing the attractiveness of turbines to tree bats may help alleviate the problem.

Journal of Wildlife Management↗

Data gap analysis for estimation of agricultural return flows in the Upper Gunnison River Basin, Colorado

The Gunnison River and many tributaries in the Upper Gunnison River Basin provide water to irrigate agricultural crops. The application of irrigation water can recharge some aquifers locally by water percolating below the root zone and eventually flowing back to the stream or river through the subsurface. Diverting surface water for irrigation reduces streamflow during the irrigation season but can provide temporary storage of water and supplement streamflow after the snowmelt runoff season. Understanding the timing and quantity of agricultural return flows could help resource managers make informed decisions and adapt to potential changes in water management and availability that could affect irrigation practices. In 2024, the U.S. Geological Survey, in cooperation with the Upper Gunnison River Water Conservancy District, began a study to characterize agricultural return flows in the Upper Gunnison River Basin by using endmember mixing analysis and developing a groundwater model. Both approaches require data from multiple sources, but data gaps exist in the East River study reach and other reaches of interest (Ohio Creek, Tomichi Creek, and Cochetopa Creek). The East River Basin, which is the initial focus of the study, has fewer data gaps than the other basins. Data gaps could be addressed by installing additional surface water and groundwater monitoring sites, making regular streamflow measurements on tributaries, and completing tests to characterize local aquifer properties.

Colorado↗