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At least 775 records · Page 43Linked to original sources

Community structure of age-0 fishes in paired mainstem and created shallow-water habitats in the Lower Missouri River

Anthropogenic alterations to aquatic ecosystems have greatly reduced and homogenized riverine habitat, especially those used by larval and juvenile fishes. Creation of shallow-water habitats is used as a restoration technique in response to altered conditions in several studies globally, but only recently in the USA. In the summer of 2012, the U.S. Army Corps of Engineers sampled larval and juvenile fishes at six paired sites (mainstem and constructed chute shallow-water habitats) along a section of the Missouri River between Rulo, NE and St. Louis, MO, USA. From those samples, we enumerated and identified a total of 7622 fishes representing 12 families. Community responses of fishes to created shallow-water habitats were assessed by comparisons of species richness and diversity measures between paired sites and among sampling events. Shannon entropy measures were transformed, and gamma diversity (total diversity) was partitioned into two components, alpha (within community) and beta (between community) diversity using a multiplicative decomposition method. Mantel test results suggest site location, time of sampling event and habitat type were drivers of larval and juvenile community structure. Paired t -test results indicated little to no differences in beta diversity between habitat types; however, chute habitats had significantly higher alpha and gamma diversity as well as increased abundances of Asian carp larvae when compared with mainstem shallow-water habitat. Our results not only show the importance of created shallow-water habitat in promoting stream fish diversity but also highlight the role space and time may play in future restoration and management efforts.

Missouri↗

Changes in backwater plant communities from 1975 to 1995 in Navigation Pool 8, Upper Mississippi River

Water elevation in the Upper Mississippi River (UMR) is highly regulated by an extensive system of locks and dams. Completion of this system in the 1930s created productive, biologically diverse backwater habitats. The status of plant communities in these backwater areas may now be threatened by several factors, including sediment accumulation, recreational use, and navigation traffic. Aerial photography, taken in 1975 and from 1991 to 1995, was used to describe vegetation changes occurring in four UMR backwater areas of Navigation Pool 8. The objectives were to determine (1) if changes occurring in these areas are consistent with hydrarch succession, (2) if the diversity of their plant communities has declined since 1975, and (3) how a large flood event that occurred in 1993 affected the composition and diversity of plant communities in these areas. Three general cover classes were recognized, representing an aquatic to terrestrial gradient. Coverages of specific vegetation types were estimated and evaluated using two indices of community diversity (vegetation richness and the Shannon diversity index). Though some vegetation changes were consistent with expected successional patterns (e.g. increased terrestrialization), other changes were not (e.g. loss of marsh vegetation). Diversity indices and coverages of most aquatic macrophytes declined from 1975 to 1991/1992 but then increased following the 1993 flood. The results suggest that disturbance–diversity concepts, including the flood pulse model, are applicable to the vegetation dynamics of these systems.

Mississippi River, Navigation Pool 8↗

Landscape and stocking effects on population genetics of Tennessee Brook Trout

Throughout their range, Brook Trout ( Salvelinus fontinalis ) occupy thousands of disjunct drainages with varying levels of disturbance, which presents substantial challenges for conservation. Within the southern Appalachian Mountains, fragmentation and genetic drift have been identified as key threats to the genetic diversity of the Brook Trout populations. In addition, extensive historic stocking of domestic lineages of Brook Trout to augment fisheries may have eroded endemic diversity and impacted locally adapted populations. We used 12 microsatellite loci to describe patterns of genetic diversity within 108 populations of wild Brook Trout from Tennessee and used linear models to explore the impacts of land use, drainage area, and hatchery stockings on metrics of genetic diversity, effective population size, and hatchery introgression. We found levels of within-population diversity varied widely, although many populations showed very limited diversity. The extent of hatchery introgression also varied across the landscape, with some populations showing high affinity to hatchery lineages and others appearing to retain their endemic character. However, we found relatively weak relationships between genetic metrics and landscape characteristics, suggesting that contemporary landscape variables are not strongly related to observed patterns of genetic diversity. We consider this result to reflect both the complex history of these populations and the challenges associated with accurately defining drainages for each population. Our study highlights the importance of genetic data to guide management decisions, as complex processes interact to shape the genetic structure of populations and make it difficult to infer the status of unsampled populations.

Tennessee↗

Dynamic Energy Budget modelling to predict eastern oyster growth, reproduction, and mortality under river management and climate change scenarios

Eastern oysters growing in deltaic Louisiana estuaries in the northern Gulf of Mexico must tolerate considerable salinity variation from natural climate variability (e.g., rainfall and stream run-off pushing isohalines offshore; tropical storms pushing isohalines inshore) and man-made diversions and siphons releasing freshwater from the Mississippi River. These salinity variations are predicted to increase with future climate change because of the increased frequency of stronger storms and also in response to proposed large-scale river diversions. Increased Mississippi River flow into coastal estuaries from river diversions, along with potential changes in rainfall and stream run-off from climate change will alter spatial and temporal salinity patterns. In this study we used an individual Dynamic Energy Budget model to predict growth and reproductive potential of eastern oysters across observed and simulated salinity gradients corresponding to different climate and river management scenarios. We used validated model outputs of salinity from a coupled hydrology-hydrodynamic model to assess the current impacts of Davis Pond diversion discharge on oysters located downstream. Under a high diversion discharge scenario oyster growth potential was reduced by 9%, 4%, and 1% in Upper, Mid, and Lower Bay locations, respectively, as compared to a limited discharge year. Reproductive outputs decreased by 34% and 2% in the Upper and Lower Bay locations, respectively, and increased by 2% at the Mid Bay site. In scenarios combining predicted increased temperature with the effect of diversions, all oysters located in the Upper and Mid Bay sites died due to severe summer conditions (high temperatures combined with low salinity). Overall, oysters in down-estuary locations, influenced by both estuarine river management and gulf conditions demonstrated significant tolerance to changing salinity and temperature conditions from diversions alone and when combined with climate change. In contrast, oysters located up-estuary, and exposed to more extreme salinity impacts from river management, demonstrated potentially lethal impacts through direct mortality, and reduced sustainability through decrease in reproductive effort. These predictions at the individual level may translate into less sustainable populations in the most extreme scenarios; restoration and production plans would benefit from accounting for these impacts on reproductive output particularly as decision makers seek to restore critical oyster areas.

Louisiana↗

Wildlife species associated with non-coniferous vegetation in Pacific Northwest conifer forests: A review

Non-coniferous vegetation, including herbs, shrubs, and broad-leaved trees, makes a vital contribution to ecosystem function and diversity in Pacific Northwest conifer forests. However, forest management has largely been indifferent or detrimental to shrubs and trees that have low commercial value, in spite of a paradigm shift towards more holistic management in recent decades. Forest management practices that are detrimental to broad-leaved trees and shrubs are likely to decrease habitat diversity for wildlife, but the number of species that may be affected has not previously been enumerated. I reviewed life history accounts for forest-dwelling vertebrate wildlife species and derived a list of 78 species in Oregon and Washington that are associated with non-coniferous vegetation. The diversity of direct and indirect food resources provided was the primary functional basis for associations of most species with non-coniferous vegetation. Thus, a diversity of herbs and broad-leaved trees and shrubs provides the foundation for food webs that contribute to diversity at multiple trophic levels in Pacific Northwest conifer forests. Given the number of species associated with non-coniferous vegetation in conifer-dominated forests, maintaining habitats that support diverse plant communities, particularly broad-leaved trees and shrubs, will be an important component of management strategies intended to foster biodiversity. Silvicultural practices such as modified planting densities, and pre-commercial and commercial thinning, can be used to control stand density in order to favor the development of understory herbs, shrubs, and a diversity of tree species within managed stands. Allowing shrubs and hardwood trees to develop and persist in early seral stands by curtailing vegetation control also would benefit many species associated with non-coniferous vegetation.

Forest Ecology and Management↗

Salinization and sedimentation drive contrasting assembly mechanisms of planktonic and sediment-bound bacterial communities in agricultural streams

Agriculture is the most dominant land use globally and is projected to increase in the future to support a growing human population but also threatens ecosystem structure and services. Bacteria mediate numerous biogeochemical pathways within ecosystems. Therefore, identifying linkages between stressors associated with agricultural land use and responses of bacterial diversity is an important step in understanding and improving resource management. Here, we use the Mississippi Alluvial Plain (MAP) ecoregion, a highly modified agroecosystem, as a case study to better understand agriculturally associated drivers of stream bacterial diversity and assembly mechanisms. In the MAP, we found that planktonic bacterial communities were strongly influenced by salinity. Tolerant taxa increased with increasing ion concentrations, likely driving homogenous selection which accounted for ~90% of assembly processes. Sediment bacterial phylogenetic diversity increased with increasing agricultural land use and was influenced by sediment particle size, with assembly mechanisms shifting from homogenous to variable selection as differences in median particle size increased. Within individual streams, sediment heterogeneity was correlated with bacterial diversity and a subsidy-stress relationship along the particle size gradient was observed. Planktonic and sediment communities within the same stream also diverged as sediment particle size decreased. Nutrients including carbon, nitrogen, and phosphorus, which tend to be elevated in agroecosystems, were also associated with detectable shifts in bacterial community structure. Collectively, our results establish that two understudied variables, salinity and sediment texture, are the primary drivers of bacterial diversity within the studied agroecosystem, whereas nutrients are secondary drivers. Although numerous macrobiological communities respond negatively, we observed increasing bacterial diversity in response to agricultural stressors including salinization and sedimentation. Elevated taxonomic and phylogenetic bacterial diversity likely increases the probability of detecting community responses to stressors. Thus, bacteria community responses may be more reliable for establishing water quality goals within highly modified agroecosystems that have experienced shifting baselines.

Mississippi↗

Using plant invasions to compare occurrence- and abundance-based calculations of biotic homogenisation: Are results complementary or contradictory?

Aim Beta diversity quantifies the similarity of ecological assemblages. Its increase, known as biotic homogenisation, can be a consequence of biological invasions. However, species occurrence (presence/absence) and abundance-based analyses can produce contradictory assessments of the magnitude and direction of changes in beta diversity. Previous work indicates these contradictions should be less frequent in nature than in theory, but a growing number of empirical studies report discrepancies between occurrence- and abundance-based approaches. Understanding if these discrepancies represent a few isolated cases or are systematic across a diversity of ecosystems would allow us to better understand the general patterns, mechanisms and impacts of biotic homogenisation. Location United States. Time Period 1963–2020. Major Taxa Studied Vascular plants. Methods We used a dataset of more than 70,000 vegetation survey plots to assess differences in biotic homogenisation with and without invasion using both occurrence- and abundance-based metrics of beta diversity. We estimated taxonomic biotic homogenisation by comparing beta diversity of invaded and uninvaded plots with both classes of metrics and investigated the characteristics of the non-native species pool that influenced the likelihood that these metrics disagree. Results In 78% of plot comparisons, occurrence- and abundance-based calculations agreed in direction, and the two metrics were generally well correlated. Our empirical results are consistent with previous theory. Discrepancies between the metrics were more likely when the same non-native species was at high cover at both plots compared for beta diversity, and when these plots were spatially distant. Main Conclusions In about 20% of cases, our calculations revealed differences in direction (homogenisation vs. differentiation) when comparing occurrence- and abundance-based metrics, indicating that the metrics are not interchangeable, especially when distances between plots are high and invader diversity is low. When data permit, combining the two approaches can offer insights into the role of invasions and extirpations in driving biotic homogenisation/differentiation.

Global Ecology and Biogeography↗

Using conservation value to assess land restoration and management alternatives across a degraded oak savanna landscape

1. Managers considering restoration of landscapes often face a fundamental challenge - what should be the habitat composition of the restored landscape? We present a method for evaluating an important conservation trade-off inherent in making that decision. 2. Oak savannas and grasslands were historically widespread across central North America but are now rare. Today, in north-west Indiana, USA, habitats spanning a range of woody vegetation density, from nearly treeless open habitats to forests, occur across the conserved landscape where savannas probably once dominated. To understand the benefits of different potential landscape compositions, we evaluated how different proportions of five habitats - open, savanna, woodland, scrub and forest - might affect the conservation value of the north-west Indiana landscape for birds. Two variables of potential conservation importance were examined: species diversity, a measure of avian community richness, and conservation index, the percentage of a bird species' global population occurring on a hectare of landscape, summed across all bird species present. Higher values of conservation index were associated with higher local densities of globally more rare and more threatened species. 3. Conservation index and species diversity were correlated negatively across hypothetical landscapes composed of different proportions of the five habitats. Therefore, a management trade-off existed between conservation index and species diversity because landscapes that maximized species diversity differed from landscapes that maximized conservation index. 4. A landscape of 50% open, 22% savanna, 15% scrub and 13% forest was predicted to represent a compromise at which conservation index and species diversity reached the same percentage of their maxima. In contrast, the current landscape is dominated by forest. 5. Synthesis and applications. We quantified the trade-off between two potential aspects of a landscape's conservation value for birds - the landscape's ability to promote avian species diversity and the landscape's use by threatened avian species. This quantification allowed us to evaluate the ability of different landscape compositions to achieve preferable trade-off compromises, such as maximizing diversity for a given level of landscape use by threatened species. Managers can use these trade-off results to determine which landscape compositions are associated with particular conservation and management priorities.

Journal of Applied Ecology↗

Postfire chaparral regeneration under mediterranean and non-mediterranean climates

This study compares postfire regeneration and diversity patterns in fire-prone chaparral shrublands from mediterranean (California) and non-mediterranean-type climates (Arizona). Vegetation sampling was conducted in tenth hectare plots with nested subplots for the first two years after fire. Floras in the two regions were compared with Jaccard's Index and importance of families and genera compared with dominance-diversity curves. Although there were 44 families in common between the two regions, the dominant families differed; Poaceae and Fabaceae in Arizona and Hydrophyllaceae and Rosaceae in California. Dominance diversity curves indicated in the first year a more equable distribution of families in Arizona than in California. Woody plants were much more dominant in the mediterranean climate and herbaceous plants more dominant in the bimodal rainfall climate. Species diversity was comparable in both regions at the lowest spatial scales but not at the tenth hectare scale. Due to the double growing season in the non-mediterranean region, the diversity for the first year comprised two different herbaceous floras in the fall and spring growing seasons. The Mediterranean climate in California, in contrast, had only a spring growing season and thus the total diversity for the first year was significantly greater in Arizona than in California for both annuals and herbaceous perennials. Chaparral in these two climate regimes share many dominant shrub species but the postfire communities are very different. Arizona chaparral has both a spring and fall growing season and these produce two very different postfire floras. When combined, the total annual diversity was substantially greater in Arizona chaparral.

Arizona, California↗

Unlearning Racism in Geoscience (URGE): Summary of U.S. Geological Survey URGE pod deliverables

The U.S. Geological Survey (USGS) is in a unique position to be a leader in diversity, equity, inclusion, and accessibility in the Earth sciences. As one of the largest geoscience employers, the USGS wields significant community influence and has a responsibility to adopt and implement robust, unbiased policies so that the science it is charged to deliver is better connected to the diverse communities it serves. Meaningful and effective improvements in implementation of diversity, equity, inclusion, and accessibility principles made within the USGS will be seen across the geoscience community. Despite this opportunity, however, the community, including the USGS, has struggled to diversify the geoscience workforce, which does not reflect the Nation’s diversity. This disparity suggests that the implementation of past policies did not achieve their desired outcomes in sustainable ways. The persistent lack of diversity across the geosciences and the racial justice protests of 2020 motivated a group of geoscience scholars to launch a global, virtual initiative known as Unlearning Racism in Geoscience (URGE) in the winter of 2021. The 16-week facilitated URGE curriculum was designed to highlight existing literature, share expert opinions, and foster discussion within groups (pods) around the world to build action plans for affecting change within their home institutions ( https://urgeoscience.org/ ). Whereas most of the approximately 4,500 participants were university faculty and students or from professional societies and nonprofit organizations, 6 pods totaling over 120 participants from the USGS joined the program—the largest participation of any Federal science agency. Summarizing the recommendations of over 100 USGS employees across the USGS who participated in URGE, this Circular represents a grassroots plan for making the USGS workforce more diverse and inclusive. To identify how peer-reviewed best practices could be implemented at the USGS, participants read existing literature, reviewed USGS policies and procedures, explored new and existing demographic data, and listened to and discussed primary interviews with scholars, all facilitated by the URGE program. This Circular leverages the efforts and momentum of the USGS URGE participants to catalyze and support positive, systemic change throughout the organization in a holistic way that represents an achievable departure from past policies that fell short of success. These recommendations are starting points. Reflecting the overall lack of diversity in the USGS workforce, the USGS URGE participants were predominantly White, meaning recommendations in this document can and should continue to evolve to include the lived perspectives and input from the broader USGS community. Finally, this summary document only highlights some of the key findings of these working groups. Additional details that may be more suitable to specific USGS Centers and programs can be found in the primary documents developed by each USGS URGE pod; a list of all registered pods can be found at https://urgeoscience.org/pods/ .

Circular↗

Conservation genomics of the Mogollon Narrow-headed gartersnake (Thamnophis rufipunctatus) and Northern Mexican gartersnake (Thamnophis eques megalops)

The ability of populations to persist and adapt to abiotic and biotic changes is reliant on genetic diversity. When connectivity across a species landscape is disrupted, the levels and distribution of genetic diversity can rapidly deteriorate as a result of genetic drift, leading to increased inbreeding and reduced adaptive potential. Therefore, understanding the distribution and degree of genetic variation within imperiled populations provides important information for conservation management and recovery strategies, especially when paired with translocation and repatriation programs. Here, we used genome-wide nuclear markers to study the population structure and genetic diversity from tissue samples collected between 2010 and 2016 of two threatened species of gartersnakes inhabiting the lower Colorado River Basin in the United States: Mogollon Narrow-headed gartersnake ( Thamnophis rufipunctatus ) and Northern Mexican gartersnake ( Thamnophis eques megalop s). Our specific objectives were to determine how populations inhabiting the lower Colorado River Basin were related to sister species and southern populations along the Sierra Madre Occidental in Mexico, to determine how genetic variation is partitioned among drainage basins in the lower Colorado River Basin, and to provide estimates of genetic diversity and effective sizes of sampled sites to aide species-specific conservation management of these threatened gartersnakes. For both species, we found that populations along the lower Colorado River Basin are highly differentiated from sister species and southern populations located further south in Mexico, and exhibit reduced genetic diversity relative to populations along the Sierra Madre Occidental. Within the lower Colorado River Basin, genetic analyses revealed highly structured genetic groups for both species of gartersnakes that point to shared contemporary and historical drivers of differentiation. We found that most sites throughout the lower Colorado River Basin have low genetic diversity and effective population sizes below the threshold required to retain adaptive potential. However, these trends were especially pronounced for T. rufipunctatus . If genetic management and translocation strategies are adopted in the future, these population genetic results can be used to highlight sites of particular concern and locate the most genetically similar sites for translocation or re-establishment efforts. Such measures could help curb further population genetic change, alleviate problems associated with low genetic diversity, and strengthen the adaptive potential across the range of these two gartersnake species.

Open-File Report↗

Phosphorus and suspended sediment load estimates for the Lower Boise River, Idaho, 1994-2002

The U.S. Geological Survey used LOADEST, newly developed load estimation software, to develop regression equations and estimate loads of total phosphorus (TP), dissolved orthophosphorus (OP), and suspended sediment (SS) from January 1994 through September 2002 at four sites on the lower Boise River: Boise River below Diversion Dam near Boise, Boise River at Glenwood Bridge at Boise, Boise River near Middleton, and Boise River near Parma. The objective was to help the Idaho Department of Environmental Quality develop and implement total maximum daily loads (TMDLs) by providing spatial and temporal resolution for phosphorus and sediment loads and enabling load estimates made by mass balance calculations to be refined and validated. Regression models for TP and OP generally were well fit on the basis of regression coefficients of determination (R2), but results varied in quality from site to site. The TP and OP results for Glenwood probably were affected by the upstream wastewater-treatment plant outlet, which provides a variable phosphorus input that is unrelated to river discharge. Regression models for SS generally were statistically well fit. Regression models for Middleton for all constituents, although statistically acceptable, were of limited usefulness because sparse and intermittent discharge data at that site caused many gaps in the resulting estimates. Although the models successfully simulated measured loads under predominant flow conditions, errors in TP and SS estimates at Middleton and in TP estimates at Parma were larger during high- and low-flow conditions. This shortcoming might be improved if additional concentration data for a wider range of flow conditions were available for calibrating the model. The average estimated daily TP load ranged from less than 250 pounds per day (lb/d) at Diversion to nearly 2,200 lb/d at Parma. Estimated TP loads at all four sites displayed cyclical variations coinciding with seasonal fluctuations in discharge. Estimated annual loads of TP ranged from less than 8 tons at Diversion to 570 tons at Parma. Annual loads of dissolved OP peaked in 1997 at all sites and were consistently higher at Parma than at the other sites. The ratio of OP to TP varied considerably throughout the year at all sites. Peaks in the OP:TP ratio occurred primarily when flows were at their lowest annual stages; estimated seasonal OP:TP ratios were highest in autumn at all sites. Conversely, when flows were high, the ratio was low, reflecting increased TP associated with particulate matter during high flows. Parma exhibited the highest OP:TP ratio during all seasons, at least 0.60 in spring and nearly 0.90 in autumn. Similar OP:TP ratios were estimated at Glenwood. Whereas the OP:TP ratio for Parma and Glenwood peaked in November or December, decreased from January through May, and increased again after June, estimates for Diversion showed nearly the opposite pattern ? ratios were highest in July and lowest in January and February. This difference might reflect complex biological and geochemical processes involving nutrient cycling in Lucky Peak Lake, but further data are needed to substantiate this hypothesis. Estimated monthly average SS loads were highest at Diversion, about 400 tons per day (ton/d). Average annual loads from 1994 through 2002 were 144,000 tons at Diversion, 33,000 tons at Glenwood, and 88,000 tons at Parma. Estimated SS loads peaked in the spring at all sites, coinciding with high flows. Increases in TP in the reach from Diversion to Glenwood ranged from 200 to 350 lb/d. Decreases in TP were small in this reach only during high flows in January and February 1997. Decreases in SS, were large during high-flow conditions indicating sediment deposition in the reach. Intermittent data at Middleton indicated that increases and decreases in TP in the reach from Glenwood to Middleton were during low- and high-flow conditions, respectively. All constituents increased in the r

Scientific Investigations Report↗

Davis Pond freshwater prediversion biomonitoring study: freshwater fisheries and eagles

In January 2001, the construction of the Davis Pond freshwater diversion structure was completed by the U.S. Army Corps of Engineers. The diversion of freshwater from the Mississippi River is intended to mitigate saltwater intrusion from the Gulf of Mexico and to lessen the concomitant loss of wetland areas. In addition to the freshwater inflow, Barataria Bay basin would receive nutrients, increased flows of sediments, and water-borne and sediment-bound compounds. The purpose of this biomonitoring study was, therefore, to serve as a baseline for prediversion concentrations of selected contaminants in bald eagle ( Haliaeetus leucocephalus ) nestlings (hereafter referred to as eaglets), representative freshwater fish, and bivalves. Samples were collected from January through June 2001. Two similarly designed postdiversion studies, as described in the biological monitoring program, are planned. Active bald eagle nests targeted for sampling eaglet blood (n = 6) were generally located southwest and south of the diversion structure. The designated sites for aquatic animal sampling were at Lake Salvador, at Lake Cataouatche, at Bayou Couba, and along the Mississippi River. Aquatic animals representative of eagle prey were collected. Fish were from three different trophic levels and have varying feeding strategies and life histories. These included herbivorous striped mullet ( Mugil cephalus ), omnivorous blue catfish ( Ictalurus furcatus ), and carnivorous largemouth bass ( Micropterus salmoides ). Three individuals per species were collected at each of the four sampling sites. Freshwater Atlantic rangia clams ( Rangia cuneata ) were collected at the downstream marsh sites, and zebra mussels ( Dreissena spp.) were collected on the Mississippi River. The U.S. Geological Survey (USGS) Biomonitoring of Environmental Status and Trends (BEST) protocols served as guides for fish sampling and health assessments. Fish are useful for monitoring aquatic ecosystems because they accumulate pesticides and other contaminants. Biomarker data on individual fish, generated at the USGS National Wetlands Research Center (Lafayette, La.), included percent white blood cells in whole blood, spleen weight to body weight ratio, liver weight to body weight ratio, condition factor, splenic macrophage aggregates, and liver microsomal 7-ethoxyresorufin-o-deethylase (EROD) activity. Fish age was estimated by comparing total lengths with values from the same species in the Southeast United States as determined from the literature. Contaminant analyses were coordinated by the U.S. Fish and Wildlife Service (USFWS) Analytical Control Facility (Laurel, Md.), where residues of organochlorine (OC) pesticides, total polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), aliphatic hydrocarbons (AHs), and trace elements were determined. The organic contaminant data were generated at the Mississippi State University Chemical Lab (Mississippi State, Miss.), and the inorganic contaminant data were generated by the Texas A&M University Geochemical and Environmental Research Group (College Station, Tex.). Statistical tests were performed to assess relationships among contaminants, fish age, fish species, and collection sites. Trends in interspecific differences among fish in concentrations of contaminants were noted. Striped mullet (hereafter mullet) frequently displayed the highest chemical concentrations. Levels of contaminants were generally higher in samples obtained from the Mississippi River than in those collected from the diversion area and were higher in mussels and clams (hereafter bivalves) than in fish. Because the Mississippi River sampling site for mullet and largemouth bass was downriver of the structure and south of New Orleans and the catfish site was upriver, the downriver data may not be directly reflective of the results from the receiving waters at the Davis Pond structure. Compared to the Caernarvon freshwater prediversion study in 1990 that assessed possible influx of contaminants with the freshwater diversion, contaminant levels in fishes and bivalves in this study were generally lower, yet three nontoxic inorganic elements in Davis Pond fish samples exhibited ranges of concentrations that were more than two times higher than did those from Caernarvon. Levels in bivalves were different between diversions but about equal in the numbers of trace elements showing high levels per location. Contaminant values were compared to those listed in various literature and agency sources, both regional and national, including the National Contaminant Biomonitoring Program (NCBP), in which the 85th percentile and above represents what is considered to be an elevated contaminant concentration and cause for concern. Generally, bivalves were at the high end of their ranges for both organic and inorganic contaminants. In this study, OCs were detectable in 67 percent of fish from the Mississippi River site, ranging from 0.15 to 1.09 μg/g wet weight (ww) or fresh weight (fw), and in 11 percent of the fish from the marsh sites, ranging from 0.06 to 0.612 μg/g ww. Bivalves from the Mississippi River had OC levels of 0.096 μg/g ww, whereas none were detectable in bivalves at the marsh sites. In this study, p,p ’-dichlorodiphenyldichloroethylene ( p,p’ -DDE) (a biodegradation product of DDT [dichlorodiphenyl trichloroethane]) and total PCBs were the most frequently detected OCs and were primarily from the Mississippi River. For total OC content, using adjusted least squares means, some significant interactions were noted between fish species and sites. PAHs were detected in aquatic animals at all sites (range of 0.017–17.534 μg/g ww), as were AHs (range of 0.423–4.549 μg/g ww); the highest levels of PAHs and AHs were found in bivalves from the Mississippi River. When analysis of variance (α = 0.05) was performed with data from aquatic animals, there were only two significant relationships between PAHs, AHs, and OCs between species, site, and age or the interaction among these variables. There was an interaction between fish species and n-decane (an AH) in that mullet and largemouth bass had significantly higher levels than did catfish ( P = 0.0175). When general linear means were used to investigate associations of inorganic contaminants among fish species, site, and age or any interactions among these variables, no significant results were noted for arsenic, cadmium, lead, beryllium, boron, molybdenum, or nickel. The range of mercury in fish in this study was 0.04–0.14 μg/g ww (0.14– 0.48 μg/g dry weight [dw]), with the most elevated levels detected in predatory largemouth bass at the sampling point farthest downstream from the structure and within the marsh area. Mercury was positively correlated with fish age ( P = 0.0152), where levels were estimated to increase 0.0253 parts per million (ppm) dw per year. In the Mississippi River, catfish showed significantly higher levels of mercury than did mullet or largemouth bass ( P = 0.00167). Among fish species, mullet displayed the highest levels in fish of aluminum, barium, manganese, and iron, all considered to have low toxicity in hydrologic systems. An interaction between fish and site was seen with aluminum ( P = 0.0031), where concentrations in mullet were significantly higher in the Mississippi River than at the other sites, as was also seen with barium ( P = 0.0009), chromium ( P = <0.0001), manganese ( P = 0.0004), strontium ( P = 0.0074), vanadium ( P = 0.0156), and zinc ( P = 0.0059). For iron ( P = 0.0.0001), mullet and largemouth bass at both the Mississippi River and Lake Salvador showed higher levels than did catfish, and these two species showed higher levels at two of the four sites. An interaction between fish and site was also seen with chromium ( P = <0.0001) in that concentrations in mullet were significantly higher in the Mississippi River than at the other sites, as was also seen with strontium ( P = 0.0074), vanadium ( P = 0.0156), and zinc ( P = 0.0059), metals for which deleterious effects have been demonstrated in other ecosystems. The NCBP program lists the 85th percentile for zinc at 34.2 μg/g fw (117.9 μg/g dw). In the Davis Pond prediversion biomonitoring study (hereafter the current study), one fish (MUL31RIVER, fish ID 8) showed values higher than that (125.4 μg/g dw or 37.54 μg/g ww), and the Mississippi River bivalve sample (MUSSRIVER) had a value of 140 μg/g dw (41.2 μg/g ww). In the current study, approximately 86 percent of the fish had measurable selenium levels, yet none reached the 85th percentile. The 85th percentile for selenium from the NCBP was 0.73 μg/g ww. Significantly higher levels of selenium were seen in mullet than in largemouth bass and catfish ( P = 0.0023). The NCBP 85th percentile for lead is 0.22 μg/g ww (0.76 μg/g dw). In the current study, the range of concentrations of lead was as much as 18.3 ppm dw (MUL31RIVER, fish ID 8), with the three most elevated values (range of 3.46–5.31 μg/g ww) coming from mullet from the Mississippi River. Biomarker data are measurable and directly reflect the condition of the animal, and measuring more than one biomarker in an individual increases confidence in health assessments. In the current study, biomarkers included macrophage aggregates (MAs), liver (hepatosomatic index [HSI]) and spleen (splenosomatic index [SSI]) weight to body weight ratios, percent white blood cells (WBCs) in whole blood, and condition factor. Few significant differences were noted with any of the biomarkers between sites, and there were no relationships between species and sites. For improved use of biomarker assessments, an increase in fish sample size would be useful for postdiversion sampling, as would comparisons of fish of the same sex and reproductive condition. During the current study, success for eagle nests in the diversion area and reference sites was similar as determined by numbers of nestlings fledged. When temperatures were below average during winter 2000, nests in both regions similarly failed. At each nest, the primary evidence of food items was small mammals. Eaglets (n = 6) generally appeared healthy, and whole blood concentrations of organic contaminants exceeded detection limits with three incidences of p,p’ -DDE (0.002–0.006 μg/L ww) and one incidence of oxychlordane (0.002 μg/L ww). The levels of p,p’ -DDE were well below those that have been inversely correlated with productivity and success rates of nesting bald eagles on a regional scale. The low values found in the whole blood samples for OC pesticides and PCBs were even lower when corrected for plasma volume. Aluminum values were 3.66 and 5.75 μg/L in two samples, zinc ranged from 5.21 to 6.77 μg/L ww in six samples, and silicon ranged from 1.7 to 4.6 μg/L in four samples. Selenium was detectable in each bird with the range at 0.332–0.566 μg/L ww, and strontium ranged from 0.0581 to 0.0975 μg/L ww. Mercury was detectable in blood samples from each bird and ranged from 0.0254 to 0.0845 μg/L ww, whereas lead was detectable in four samples and ranged from 0.0042 to 0.0136 μg/L ww. Although no detectable levels of total PCBs were found (also correlated with decreased reproductive productivity), 70 percent of the aquatic animals from the Mississippi River contained total PCBs (range 0.13–0.79 μg/L), whereas only about 7 percent of the aquatic animals sampled from the marsh area contained PCBs. Suggestions for postdiversion sampling include lowering the analytical detection limit for some metals, sampling aquatic animals over the course of a single season, obtaining a higher sample number of mature fish of one species (for example, blue catfish) within a range of total lengths for biomarker analyses, obtaining otoliths for estimating fish ages, assessing dioxins in eaglet blood, examining triazines in water, and obtaining all Mississippi River fish samples as close to the Davis Pond structure intake as possible. Because contaminants found in blood of eaglets reflect their prey species and because of the contaminant levels found in fish in the current study, eaglets may not be consuming primarily these species; therefore, obtaining juvenile nutria ( Myocastor coypus ) or turtle species for contaminant analyses might be considered, as well as collecting greater blood volume and using plasma to measure OCs and PCBs. Data obtained postdiversion will be compared with prediversion data to monitor changes.

Louisiana↗

Temporal species richness-biomass relationships along successional gradients

Diversity-biomass relationships are frequently reported to be hump-shaped over space at a given time. However, it is not yet clear how diversity and biomass change simultaneously and how they are related to each other over time (e.g. in succession) at one locality. This study develops a temporal model based on the projected changes of various community variables in a generalized terrestrial environment after fire and uses post-fire succession data on Santa Monica Mountains of southern California and other published succession data to examine the temporal diversity-biomass relationships. The results indicate that in the early stages of succession, both diversity and biomass increase and a positive relationship appears, while in the late stages of succession, biomass continued to increase but diversity usually declines; thus a negative relationship may be observed. When the scales of measurement become sufficiently large so that the measured diversity and biomass cross various stages of succession, a 'hump-shaped' relationship can emerge. The diversity-biomass relationship appears to be concordant in space and time when appropriate scales are used. Formerly proposed explanations for spatial patterns may well apply to the temporal patterns (particularly colonization, facilitation and competitive exclusion).

Journal of Vegetation Science↗

Temporal species richness-biomass relationships along successional gradients

Diversity-biomass relationships are frequently reported to be hump-shaped over space at a given time. However, it is not yet clear how diversity and biomass change simultaneously and how they are related to each other overtime (e.g. in succession) at one locality. This study develops a temporal model based on the projected changes of various community variables in a generalized terrestrial environment after fire and uses post-fire succession data on Santa Monica Mountains of southern California and other published succession data to examine the temporal diversity-biomass relationships. The results indicate that in the early stages of succession, both diversity and biomass increase and a positive relationship appears, while in the late stages of succession, biomass continued to increase but diversity usually declines; thus a negative relationship may be observed. When the scales of measurement become sufficiently large so that the measured diversity and biomass cross various stages of succession, a 'hump-shaped' relationship can emerge. The diversity-biomass relationship appears to be concordant in space and time when appropriate scales are used. Formerly proposed explanations for spatial patterns may well apply to the temporal patterns (particularly colonization, facilitation and competitive exclusion).

Journal of Vegetation Science↗

Genetic conservation and paddlefish propagation

The conservation of genetic diversity of our natural resources is overwhelmingly one of the central foci of 21st century management practices. Three recommendations related to the conservation of paddlefish Polyodon spathula genetic diversity are to (1) identify genetic diversity at both nuclear and mitochondrial DNA loci using a suggested list of 20 sampling locations, (2) use genetic diversity estimates to develop genetic management units, and (3) identify broodstock sources to minimize effects of supplemental stocking on the genetic integrity of native paddlefish populations. We review previous genetic work on paddlefish and described key principles and concepts associated with maintaining genetic diversity within and among paddlefish populations and also present a genetic case study of current paddlefish propagation at the U.S. Fish and Wildlife Service Gavins Point National Fish Hatchery. This study confirmed that three potential sources of broodfish were genetically indistinguishable at the loci examined, allowing the management agencies cooperating on this program flexibility in sampling gametes. This study also showed significant bias in the hatchery occurred in terms of male reproductive contribution, which resulted in a shift in the genetic diversity of progeny compared to the broodfish. This shift was shown to result from differential male contributions, partially attributed to the mode of egg fertilization. Genetic insights enable implementation of a paddlefish propagation program within an adaptive management strategy that conserves inherent genetic diversity while achieving demographic goals.

American Fisheries Society Symposium↗

Sub-indicator: Prey fish

Prey fish communities across the Great Lakes continue to change, although the direction and magnitude of those changes are not consistent across the lakes. The metrics used to categorize prey fish status in this and previous periods are based on elements that are common among each of the lake’s Fish Community Objectives and include diversity and the relative role of native species in the prey fish communities. The diversity index categorized three of lakes as ‘fair’, while Superior and Erie were ‘good’ (Table 1). The short term trend, from the previous period (2008-2010) to the current period (2011-2014) found diversity in Erie and Superior to be unchanging, but the other three lakes to be ‘deteriorating’, resulting in an overall trend categorization of ‘undetermined’ (Table 1). The long term diversity trend suggested Lakes Superior and Erie have the most diverse prey communities although the index for those prey fish have been quite variable over time (Figure 1). In Lake Huron, where non-native alewife have substantially declined, the diversity index has also declined. The continued dominance of alewife in Lake Ontario (96% of the prey fish biomass) resulted in the lowest diversity index value (Figure 1). The proportion of native species within the community was judged as ‘good’ in Lakes Superior and Huron, ‘fair’ in Michigan and Erie and ‘poor’ in Ontario (Table 2). The short term trend was improving in in all lakes except Michigan (‘deteriorating’) and Ontario (‘unchanging’), resulting in an overall short term trend of ‘undetermined’ (Table 2). Over the current period, Lake Superior consistently had the highest proportion native prey fish (87%) while Lake Ontario had the lowest (1%) (Figure 2). Lake Michigan’s percent native has declined as round goby increase and comprises a greater proportion of the community. Native prey fish make up 51% of Lake Erie, although basin-specific values differed (Figure 2). Most notably, native species in Lake Huron comprised less than 10% of the community in 1970, but since alewife have declined, now represent nearly 80% of the community (Figure 2). Prey fish data are most consistent for in-lake populations, which are reported here; data from connecting channels was not consistently available across the basin. Abundance was not used to judge prey fish status since successful, basin-wide management actions, including mineral nutrient input reductions and piscivore restoration, both inherently reduce prey fish abundance. However, recent abundance trends as they relate to predator prey balance are referenced, such as in Lakes Michigan and Huron where piscivore stocking is being reduced to lower predation demand on prey fish populations and maintain sport fisheries.

Great Lakes↗

Comparative phylogeography reveals deep lineages and regional evolutionary hotspots in the Mojave and Sonoran Deserts

Aim: We explored lineage diversification within desert-dwelling fauna. Our goals were (1) to determine whether phylogenetic lineages and population expansions were consistent with younger Pleistocene climate fluctuation hypotheses or much older events predicted by pre-Pleistocene vicariance hypotheses, (2) to assess concordance in spatial patterns of genetic divergence and diversity among species and (3) to identify regional evolutionary hotspots of divergence and diversity and assess their conservation status. Location: Mojave, Colorado, and Sonoran Deserts, USA. Methods: We analysed previously published gene sequence data for twelve species. We used Bayesian gene tree methods to estimate lineages and divergence times. Within each lineage, we tested for population expansion and age of expansion using coalescent approaches. We mapped interpopulation genetic divergence and intra-population genetic diversity in a GIS to identify hotspots of highest genetic divergence and diversity and to assess whether protected lands overlapped with evolutionary hotspots. Results: In seven of the 12 species, lineage divergence substantially predated the Pleistocene. Historical population expansion was found in eight species, but expansion events postdated the Last Glacial Maximum (LGM) in only four. For all species assessed, six hotspots of high genetic divergence and diversity were concentrated in the Colorado Desert, along the Colorado River and in the Mojave/Sonoran ecotone. At least some proportion of the land within each recovered hotspot was categorized as protected, yet four of the six also overlapped with major areas of human development. Main conclusions: Most of the species studied here diversified into distinct Mojave and Sonoran lineages prior to the LGM – supporting older diversification hypotheses. Several evolutionary hotspots were recovered but are not strategically paired with areas of protected land. Long-term preservation of species-level biodiversity would entail selecting areas for protection in Mojave and Sonoran Deserts to retain divergent genetic diversity and ensure connectedness across environmental gradients.

Colorado Desert;Mojave Desert;Sonoran Desert↗