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At least 757 records · Page 42Linked to original sources

Persistence and potential effects of complex organic contaminant mixtures in wastewater-impacted streams

Natural and synthetic organic contaminants in municipal wastewater treatment plant (WWTP) effluents can cause ecosystem impacts, raising concerns about their persistence in receiving streams. In this study, Lagrangian sampling, in which the same approximate parcel of water is tracked as it moves downstream, was conducted at Boulder Creek, Colorado and Fourmile Creek, Iowa to determine in-stream transport and attenuation of organic contaminants discharged from two secondary WWTPs. Similar stream reaches were evaluated, and samples were collected at multiple sites during summer and spring hydrologic conditions. Travel times to the most downstream (7.4 km) site in Boulder Creek were 6.2 h during the summer and 9.3 h during the spring, and to the Fourmile Creek 8.4 km downstream site times were 18 and 8.8 h, respectively. Discharge was measured at each site, and integrated composite samples were collected and analyzed for >200 organic contaminants including metal complexing agents, nonionic surfactant degradates, personal care products, pharmaceuticals, steroidal hormones, and pesticides. The highest concentration (>100 μg L –1 ) compounds detected in both WWTP effluents were ethylenediaminetetraacetic acid and 4-nonylphenolethoxycarboxylate oligomers, both of which persisted for at least 7 km downstream from the WWTPs. Concentrations of pharmaceuticals were lower (<1 μg L –1 ), and several compounds, including carbamazepine and sulfamethoxazole, were detected throughout the study reaches. After accounting for in-stream dilution, a complex mixture of contaminants showed little attenuation and was persistent in the receiving streams at concentrations with potential ecosystem implications.

Colorado, Iowa↗

Tracking nonpoint source nitrogen pollution in human-impacted watersheds

Nonpoint source nitrogen (N) pollution is a leading contributor to U.S. water quality impairments. We combined watershed N mass balances and stable isotopes to investigate fate and transport of nonpoint N in forest, agricultural, and urbanized watersheds at the Baltimore Long-Term Ecological Research site. Annual N retention was 55%, 68%, and 82% for agricultural, suburban, and forest watersheds, respectively. Analysis of &delta; 15 N-NO 3 &ndash; , and &delta; 18 O-NO 3 &ndash; indicated wastewater was an important nitrate source in urbanized streams during baseflow. Negative correlations between &delta; 15 N-NO 3 &ndash; and &delta; 18 O-NO 3 &ndash; in urban watersheds indicated mixing between atmospheric deposition and wastewater, and N source contributions changed with storm magnitude (atmospheric sources contributed &sim;50% at peak storm N loads). Positive correlations between &delta; 15 N-NO 3 &ndash; and &delta; 18 O-NO 3 &ndash; in watersheds suggested denitrification was removing septic system and agriculturally derived N, but N from belowground leaking sewers was less susceptible to denitrification. N transformations were also observed in a storm drain (no natural drainage network) potentially due to organic carbon inputs. Overall, nonpoint sources such as atmospheric deposition, wastewater, and fertilizer showed different susceptibility to watershed N export. There were large changes in nitrate sources as a function of runoff, and anticipating source changes in response to climate and storms will be critical for managing nonpoint N pollution.

Environmental Science & Technology↗

Geochemistry of inorganic nitrogen in waters released from coal-bed natural gas production wells in the Powder River Basin, Wyoming

Water originating from coal-bed natural gas (CBNG) production wells typically contains ammonium and is often disposed via discharge to ephemeral channels. A study conducted in the Powder River Basin, Wyoming, documented downstream changes in CBNG water composition, emphasizing nitrogen-cycling processes and the fate of ammonium. Dissolved ammonium concentrations from 19 CBNG discharge points ranged from 95 to 527 μM. Within specific channels, ammonium concentrations decreased with transport distance, with subsequent increases in nitrite and nitrate concentrations. Removal efficiency, or uptake, of total dissolved inorganic nitrogen (DIN) varied between channel types. DIN uptake was greater in the gentle-sloped, vegetated channel as compared to the incised, steep, and sparsely vegetated channel and was highly correlated with diel patterns of incident light and dissolved oxygen concentration. In a larger main channel with multiple discharge inputs ( n = 13), DIN concentrations were >300 μM, with pH > 8.5, after 5 km of transport. Ammonium represented 25−30% of the large-channel DIN, and ammonium concentrations remained relatively constant with time, with only a weak diel pattern evident. In July 2003, the average daily large-channel DIN load was 23 kg N day −1 entering the Powder River, an amount which substantially increased the total Powder River DIN load after the channel confluence. These results suggest that CBNG discharge may be an important source of DIN to western watersheds, at least at certain times of the year, and that net oxidation and/or removal is dependent upon the extent of contact with sediment and biomass, type of drainage channel, and time of day.

Wyoming↗

Metolachlor and alachlor breakdown product formation patterns in aquatic field mesocosms

The transformation of metolachlor [2-chloro- N -(2-ethyl-6-methylphenyl)- N -(2-methoxy-1-methylethyl)acetamide] and alachlor [2-chloro- N -(2,6-diethylphenyl)- N -(methoxymethyl)acetamide] in aquatic systems was investigated using outdoor tank mesocosms. Metolachlor and alachlor levels and their ethane sulfonic acid (ESA) and oxanillic acid breakdown products were monitored over time under five experimental treatments (each in quadruplicate). Background water conditions were identical in all treatments with each treatment differing based on the level and type(s) of herbicide present. Treatments included a no-herbicide control, 10 μg/L metolachlor, 25 μg/L metolachlor, 25 μg/L alachlor, and 25 μg/L alachlor plus 25 μg/L metolachlor in combination. The experiment was initiated by adding herbicide(s) to the units to the target concentrations; herbicide and breakdown product levels and other chemical parameters were then monitored for 85 days. In general, metolachlor half-lives were longer than alachlor half-lives under all treatments, although the differences were not statistically significant. Metolachlor half-lives (±95% confidence limits) ranged from 33.0 d (±14.1 d) to 46.2 d (±40.0 d), whereas alachlor half-lives ranged from 18.7 d (±3.5 d) to 21.0 d (±6.5 d) for different treatments. Formation patterns of ESA were similar in all treatments, whereas oxanillic acid formation differed for the two herbicides. Alachlor oxanillic acid was produced in larger quantities than metolachlor oxanillic acid and either ESA under equivalent conditions. Our results suggest that the transformation pathways for alachlor and metolachlor in aquatic systems are similar and resemble the acetochlor pathway in soils proposed by Feng ( Pestic. Biochem. Physiol . 1991 , 34 , 136); however, the oxanillic acid branch of the pathway is favored for alachlor as compared with metolachlor.

Environmental Science & Technology↗

Centurial persistence of forever chemicals at military fire training sites

Drinking water contamination by per- and polyfluoroalkyl substances (PFAS) is widespread near more than 300 United States (U.S.) military bases that used aqueous film-forming foams (AFFF) for fire training and firefighting activities. Much of the PFAS at these sites consist of precursors that can transform into terminal compounds of known health concern but are omitted from standard analytical methods. Here, we estimate the expected duration and contribution of precursor biotransformation to groundwater PFAS contamination at an AFFF-contaminated military base on Cape Cod, Massachusetts, United States, by optimizing a geochemical box model using measured PFAS concentrations from a multidecadal time series of groundwater and a soil survey in the source zone. A toolbox of analytical techniques used to reconstruct the mass budget of PFAS showed that precursors accounted for 46 ± 8% of the extractable organofluorine (a proxy for total PFAS) across years. Terminal PFAS still exceed regulatory limits by 2000-fold decades after AFFF use ceased. Measurements and numerical modeling show that sulfonamido precursors are retained in the vadose zone and their slow biotransformation into perfluoroalkyl sulfonates (half-life > 66 yr) sustains groundwater concentrations of perfluorobutane sulfonate (PFBS) and perfluorohexane sulfonate (PFHxS). The estimated PFAS reservoir in the vadose zone and modeled flux into groundwater suggest PFAS contamination above regulatory guidelines will persist for centuries without remediation.

Massachusetts↗

Characterizing contaminant concentrations with depth by using the USGS well profiler in Oklahoma, 2003-9

Since 2003, the U.S. Geological Survey (USGS) Oklahoma Water Science Center has been using the USGS well profiler to characterize changes in water contribution and contaminant concentrations with depth in pumping public-supply wells in selected aquifers. The tools and methods associated with the well profiler, which were first developed by the USGS California Water Science Center, have been used to investigate common problems such as saline water intrusion in high-yield irrigation wells and metals contamination in high-yield public-supply wells. The USGS well profiler is a slim (less than 1 inch in diameter), high-pressure hose that can be raised and lowered between the production pipe and casing (or borehole) of a well by using a motorized hose reel. Use of this tool is considerably less expensive than use of standard methods of depth-dependent sampling, and the USGS well profiler generally requires less downtime of the well. In terms of data quality, the greatest advantage of the USGS well profiler is that all data collection is performed under production pumping rates. In Oklahoma, the USGS well profiler has been modified and adapted for use in low-yield (150?350 gallons per minute) wells of various construction types common in Oklahoma. This tool has been used in selected public-supply wells in Hinton, Moore, and Norman to identify which producing zones are contaminated by naturally occurring arsenic. The tool and method also can be used to investigate other nonvolatile contaminants of interest, including uranium, radium, barium, boron, lead, selenium, sulfate, chloride, fluoride, nitrate, and chromium. In 2007, the USGS well profiler was used to investigate saline water intrusion in a deep public-supply well completed in the Ozark (Roubidoux) aquifer. In northeast Oklahoma, where the Ozark aquifer is known to be susceptible to contamination from mining activities, the well profiler also could be used to investigate sources (depths) of metals contamination and to identify routes of entry of metals to production wells.Water suppliers can consider well rehabilitation as a potential remediation strategy because of the ability to identify changes in contaminant concentrations with depth in individual wells with the USGS well profiler. Well rehabilitation methods, which are relatively inexpensive compared to drilling and completing new wells, involve modifying the construction or operation of a well to enhance the production of water from zones with lesser concentrations of a contaminant or to limit the production of water from zones with greater concentrations of a contaminant. One of the most effective well rehabilitation methods is zonal isolation, in which water from contaminated zones is excluded from production through installation of cement plugs or packers. By using relatively simple and inexpensive well rehabilitation methods, water suppliers may be able to decrease exposure of customers to contaminants and avoid costly installation of additional wells, conveyance infrastructure, and treatment technologies.

Fact Sheet↗

Relationship and variation of qPCR and culturable enterococci estimates in ambient surface waters are predictable

The quantitative polymerase chain reaction (qPCR) method provides rapid estimates of fecal indicator bacteria densities that have been indicated to be useful in the assessment of water quality. Primarily because this method provides faster results than standard culture-based methods, the U.S. Environmental Protection Agency is currently considering its use as a basis for revised ambient water quality criteria. In anticipation of this possibility, we sought to examine the relationship between qPCR-based and culture-based estimates of enterococci in surface waters. Using data from several research groups, we compared enterococci estimates by the two methods in water samples collected from 37 sites across the United States. A consistent linear pattern in the relationship between cell equivalents (CCE), based on the qPCR method, and colony-forming units (CFU), based on the traditional culturable method, was significant (P < 0.05) at most sites. A linearly decreasing variance of CCE with increasing CFU levels was significant (P < 0.05) or evident for all sites. Both marine and freshwater sites under continuous influence of point-source contamination tended to reveal a relatively constant proportion of CCE to CFU. The consistency in the mean and variance patterns of CCE versus CFU indicates that the relationship of results based on these two methods is more predictable at high CFU levels (e.g., log 10 CFU > 2.0/100 mL) while uncertainty increases at lower CFU values. It was further noted that the relative error in replicated qPCR estimates was generally higher than that in replicated culture counts even at relatively high target levels, suggesting a greater need for replicated analyses in the qPCR method to reduce relative error. Further studies evaluating the relationship between culture and qPCR should take into account analytical uncertainty as well as potential differences in results of these methods that may arise from sample variability, different sources of pollution, and environmental factors.

Environmental Science & Technology↗

The role of the Everglades Mangrove Ecotone Region (EMER) in regulating nutrient cycling and wetland productivity in South Florida

The authors summarize the main findings of the Florida Coastal Everglades Long-Term Ecological Research (FCE-LTER) program in the EMER, within the context of the Comprehensive Everglades Restoration Plan (CERP), to understand how regional processes, mediated by water flow, control population and ecosystem dynamics across the EMER landscape. Tree canopies with maximum height <3 m cover 49% of the EMER, particularly in the SE region. These scrub/dwarf mangroves are the result of a combination of low soil phosphorus (P < 59 μg P g dw −1 ) in the calcareous marl substrate and long hydroperiod. Phosphorus limits the EMER and its freshwater watersheds due to the lack of terrigenous sediment input and the phosphorus-limited nature of the freshwater Everglades. Reduced freshwater delivery over the past 50 years, combined with Everglades compartmentalization and a 10 cm rise in coastal sea level, has led to the landward transgression (∼1.5 km in 54 years) of the mangrove ecotone. Seasonal variation in freshwater input strongly controls the temporal variation of nitrogen and P exports (99%) from the Everglades to Florida Bay. Rapid changes in nutrient availability and vegetation distribution during the last 50 years show that future ecosystem restoration actions and land use decisions can exert a major influence, similar to sea level rise over the short term, on nutrient cycling and wetland productivity in the EMER.

Florida↗

Regional patterns in the isotopic composition of natural and anthropogenic nitrate in groundwater, High Plains, U.S.A.

Mobilization of natural nitrate (NO 3 - ) deposits in the subsoil by irrigation water in arid and semiarid regions has the potential to produce large groundwater NO 3 - concentrations. The use of isotopes to distinguish between natural and anthropogenic NO 3 - sources in these settings could be complicated by the wide range in δ 15 N values of natural NO 3 - . An ∼10 000 year record of paleorecharge from the regionally extensive High Plains aquifer indicates that δ 15 N values for NO 3 - derived from natural sources ranged from 1.3 to 12.3‰ and increased systematically from the northern to the southern High Plains. This collective range in δ 15 N values spans the range that might be interpreted as evidence for fertilizer and animal-waste sources of NO 3 - ; however, the δ 15 N values for NO 3 - in modern recharge ( less than 50 years) under irrigated fields were, for the most part, distinctly different from those of paleorecharge when viewed in the overall regional context. An inverse relation was observed between the δ 15 N[NO 3 - ] values and the NO 3 - /Cl - ratios in paleorecharge that is qualitatively consistent with fractionating losses of N increasing from north to south in the High Plains. N and O isotope data for NO 3 - are consistent with both NH 3 volatilization and denitrification, having contributed to fractionating losses of N prior to recharge. The relative importance of different isotope fractionating processes may be influenced by regional climate patterns as well as by local variation in soils, vegetation, topography, and moisture conditions.

Environmental Science & Technology↗

Estimation of dynamic geologic CO2 storage resources in the Illinois Basin, including effects of brine extraction, anisotropy, and hydrogeologic heterogeneity

Since the vast majority of carbon dioxide (CO 2 ) storage resources in the United States are in deep saline aquifers, optimizing the use of these saline storage resources could be crucial for efficient development of geologic CO 2 storage (GCS) resources and basin- or larger-scale deployment of GCS in the country. Maximum CO 2 injection rates can be enhanced by extracting brine from the CO 2 storage unit. However, disposal of the extracted brine is both a technological and economic challenge. The lowest-cost option would likely be reinjection of the extracted brine into another formation above or below the CO 2 storage unit. Therefore, it is important to estimate brine injectivity as it will constrain the potential to increase CO 2 injectivity at an injection site that has access to multiple geologic storage units where either CO 2 or brine can be injected. Using a simulation-optimization framework, coupled with a non-isothermal, multiphase CO 2 -water-salt equation-of-state module, we developed a computationally efficient method for evaluating optimization of simultaneous CO 2 injection, brine extraction, and brine (re)injection at hypothetical injection sites deployed across a geologic basin. The Illinois basin is ideal for testing our methodology because it contains multiple geologic storage units with seals in between them to isolate injection of CO 2 in one unit from interfering with the injection of either brine or CO 2 in another unit above or below it. In addition, we investigated the relative effects of variation in key geologic parameters as well as two reservoir structures (hydrogeologic heterogeneity/anisotropy and homogeneity/isotropy) on CO 2 injectivities and enhancement of CO 2 injectivity through extracting brine. Results suggest that permeability, depth, and especially thickness of the storage unit could be the most influential parameters determining CO 2 injectivity. They also suggest that only injecting CO 2 into the storage unit with the greatest injectivity, enhancing that unit’s injectivity by extracting brine, and disposing of the produced brine in other suitable units could maximize total CO 2 injectivity in limited regions of the basin. At the majority of simulated injection sites, however, we found that injecting CO 2 into all of the accessible and suitable storage units was more likely to maximize the CO 2 storage resource.

Illinois, Kentucky, Indiana↗

Using chromium stable isotope ratios to quantify Cr(VI) reduction: Lack of sorption effects

Chromium stable isotope values can be effectively used to monitor reduction of Cr(VI) in natural waters. We investigate effects of sorption during transport of Cr(VI) which may also shift Cr isotopes values, complicating efforts to quantify reduction. This study shows that Cr stable isotope fractionation caused by sorption is negligible. Equilibrium fractionation of Cr stable isotopes between dissolved Cr(VI) and Cr(VI) adsorbed onto γ-Al 2 O 3 and goethite is less than 0.04‰ ( 53 Cr/ 52 Cr) under environmentally relevant pH conditions. Batch experiments at pH 4.0 and pH 6.0 were conducted in series to sequentially magnify small isotope fractionations. A simple transport model suggests that adsorption may cause amplification of a small isotope fractionation along extreme fringes of a plume, leading to shifts in 53 Cr/ 52 Cr values. We therefore suggest that isotope values at extreme fringes of Cr plumes be critically evaluated for sorption effects. A kinetic effect was observed in experiments with goethite at pH 4 where apparently lighter isotopes diffuse into goethite clumps at a faster rate before eventually reaching equilibrium. This observed kinetic effect may be important in a natural system that has not attained equilibrium and is in need of further study. Cr isotope fractionation caused by speciation of Cr(VI) between HCrO 4 - and CrO 4 2 - was also examined, and we conclude that it is not measurable. In the absence of isotope fractionation caused by equilibrium speciation and sorption, most of the variation in δ 53 Cr values may be attributed to reduction, and reliable estimates of Cr reduction can be made.

Environmental Science & Technology↗

Incidence of the enterococcal surface protein ( esp ) gene in human and animal fecal sources

The occurrence of the enterococcal surface protein ( esp ) gene in the opportunistic pathogens Enterococcus faecalis and E. faecium is well-documented in clinical research. Recently, the esp gene has been proposed as a marker of human pollution in environmental waters; however, information on its relative incidence in various human and animal fecal sources is limited. We have determined the occurrence of the esp gene in enterococci from human ( n = 64) and animal ( n = 233) fecal samples by polymerase chain reaction using two primer sets: one presumably specific for E. faecium ( esp fm ) and the other for both E. faecalis and E. faecium ( esp fs/fm ). We believe that this research is the first to explore the use of esp fs/fm for the detection of human waste in natural environmental settings. The incidence in human sources was 93.1% esp fm and 100% esp fs/fm in raw sewage influent; 30% for both esp fm and esp fs/fm in septic waste; and 0% esp fm and 80% esp fs/fm in active pit toilets. The overall occurrence of the gene in animal feces was 7.7% ( esp fs/fm ) and 4.7% ( esp fm ); animal types with positive results included dogs (9/43, all esp fm ), gulls (10/34, esp fs/fm ; 2/34, esp fm ), mice (3/22, all esp fs/fm ), and songbirds (5/55, all esp fs/fm ). The esp gene was not detected in cat (0/34), deer (0/4), goose (0/18), or raccoon (0/23) feces. The inconsistent occurrence, especially in septic and pit toilet sewage, suggests a low statistical power of discrimination between animal and human sources, which means a large number of replicates should be collected. Both esp fm and esp fs/fm were common in raw sewage, but neither one efficiently differentiated between animal and other human sources.

Environmental Science & Technology↗

Natural chlorate in the environment: Application of a new IC-ESI/MS/MS method with a Cl18O3- internal standard

A new ion chromatography electrospray tandem mass spectrometry (IC-ESI/MS/MS) method has been developed for quantification and confirmation of chlorate (ClO 3 − ) in environmental samples. The method involves the electro-chemical generation of isotopically labeled chlorate internal standard (Cl 18 O 3 − ) using 18 O water (H 2 18 O). The standard was added to all samples prior to analysis thereby minimizing the matrix effects that are associated with common ions without the need for expensive sample pretreatments. The method detection limit (MDL) for ClO 3 − was 2 ng L −1 for a 1 mL volume sample injection. The proposed method was successfully applied to analyze ClO 3 − in difficult environmental samples including soil and plant leachates. The IC-ESI/MS/MS method described here was also compared to established EPA method 317.0 for ClO 3 − analysis. Samples collected from a variety of environments previously shown to contain natural perchlorate (ClO 4 − ) occurrence were analyzed using the proposed method and ClO 3 − was found to co-occur with ClO 4 − at concentrations ranging from <2 ng L −1 in precipitation from Texas and Puerto Rico to >500 mg kg −1 in caliche salt deposits from the Atacama Desert in Chile. Relatively low concentrations of ClO 3 − in some natural groundwater samples (<0.1 μg L −1 ) analyzed in this work may indicate lower stability when compared to ClO 4 − in the subsurface. The high concentrations of ClO 3 − in caliches and soils (3−6 orders of magnitude greater) as compared to precipitation samples indicate that ClO 3 − , like ClO 4 − , may be atmospherically produced and deposited, then concentrated in dry soils, and is possibly a minor component in the biogeochemical cycle of chlorine.

Environmental Science & Technology↗

Contaminant exposure and transport from three potential reuse waters within a single watershed

Global demand for safe and sustainable water supplies necessitates a better understanding of contaminant exposures in potential reuse waters. In this study, we compared exposures and load contributions to surface water from the discharge of three reuse waters (wastewater effluent, urban stormwater, and agricultural runoff). Results document substantial and varying organic-chemical contribution to surface water from effluent discharges (e.g., disinfection byproducts [DBP], prescription pharmaceuticals, industrial/household chemicals), urban stormwater (e.g., polycyclic aromatic hydrocarbons, pesticides, nonprescription pharmaceuticals), and agricultural runoff (e.g., pesticides). Excluding DBPs, episodic storm-event organic concentrations and loads from urban stormwater were comparable to and often exceeded those of daily wastewater-effluent discharges. We also assessed if wastewater-effluent irrigation to corn resulted in measurable effects on organic-chemical concentrations in rain-induced agricultural runoff and harvested feedstock. Overall, the target-organic load of 491 g from wastewater-effluent irrigation to the study corn field during the 2019 growing season did not produce substantial dissolved organic-contaminant contributions in subsequent rain-induced runoff events. Out of the 140 detected organics in source wastewater-effluent irrigation, only imidacloprid and estrone had concentrations that resulted in observable differences between rain-induced agricultural runoff from the effluent-irrigated and nonirrigated corn fields. Analyses of pharmaceuticals and per-/polyfluoroalkyl substances in at-harvest corn-plant samples detected two prescription antibiotics, norfloxacin and ciprofloxacin, at concentrations of 36 and 70 ng/g, respectively, in effluent-irrigated corn-plant samples; no contaminants were detected in noneffluent irrigated corn-plant samples.

Oklahoma↗

Association of toxin-producing Clostridium botulinum with the macroalga Cladophora in the Great Lakes

Avian botulism, a paralytic disease of birds, often occurs on a yearly cycle and is increasingly becoming more common in the Great Lakes. Outbreaks are caused by bird ingestion of neurotoxins produced by Clostridium botulinum, a spore-forming, gram-positive, anaerobe. The nuisance, macrophytic, green alga Cladophora (Chlorophyta; mostly Cladophora glomerata L.) is a potential habitat for the growth of C. botulinum. A high incidence of botulism in shoreline birds at Sleeping Bear Dunes National Lakeshore (SLBE) in Lake Michigan coincides with increasingly massive accumulations of Cladophora in nearshore waters. In this study, free-floating algal mats were collected from SLBE and other shorelines of the Great Lakes between June and October 2011. The abundance of C. botulinum in algal mats was quantified and the type of botulism neurotoxin (bont) genes associated with this organism were determined by using most-probable-number PCR (MPN-PCR) and five distinct bont gene-specific primers (A, B, C, E, and F). The MPN-PCR results showed that 16 of 22 (73%) algal mats from the SLBE and 23 of 31(74%) algal mats from other shorelines of the Great Lakes contained the bont type E (bont/E) gene. C. botulinum was present up to 15 000 MPN per gram dried algae based on gene copies of bont/E. In addition, genes for bont/A and bont/B, which are commonly associated with human diseases, were detected in a few algal samples. Moreover, C. botulinum was present as vegetative cells rather than as dormant spores in Cladophora mats. Mouse toxin assays done using supernatants from enrichment of Cladophora containing high densities of C. botulinum (>1000 MPN/g dried algae) showed that Cladophora-borne C. botulinum were toxin-producing species (BoNT/E). Our results indicate that Cladophora provides a habitat for C. botulinum, warranting additional studies to better understand the relationship between this bacterium and the alga, and how this interaction potentially contributes to botulism outbreaks in birds.

Great Lakes↗

Earthquake shaking and damage to buildings

Ground shaking close to the causative fault of an earthquake is more intense than it was previously believed to be. This raises the possibility that large numbers of buildings and other structures are not sufficiently resistant for the intense levels of shaking that can occur close to the fault. Many structures were built before earthquake codes were adopted; others were built according to codes formulated when less was known about the intensity of near-fault shaking. Although many building types are more resistant than conventional design analyses imply, the margin of safety is difficult to quantify. Many modern structures, such as freeways, have not been subjected to and tested by near-fault shaking in major earthquakes (magnitude 7 or greater). Damage patterns in recent moderate-sized earthquakes occurring in or adjacent to urbanized areas, however, indicate that many structures, including some modern ones designed to meet earthquake code requirements, cannot withstand the severe shaking that can occur close to a fault. It is necessary to review the ground motion assumed and the methods utilized in the design of important existing structures and, if necessary, to strengthen or modify the use of structures that are found to be weak. New structures situated close to active faults should be designed on the basis of ground motion estimates greater than those used in the past. The ultimate balance between risk of earthquake losses and cost for both remedial strengthening and improved earthquake-resistant construction must be decided by the public. Scientists and engineers must inform the public about earthquake shaking and its effect on structures. The exposure to damage from seismic shaking is steadily increasing because of continuing urbanization and the increasing complexity of lifeline systems, such as power, water, transportation, and communication systems. In the near future we should expect additional painful examples of the damage potential of moderate-sized earthquakes in urban areas. Over a longer time span, however, we can significantly reduce the risk to life and property from seismic shaking through better land utilization, improved building codes and construction practices, and at least the gradual replacement of poor buildings by more resistant buildings. Progress toward reducing risk from seismic shaking through better building design is slowed by deficiencies in our knowledge about the nature of damaging ground motion and the failure mechanisms in structures. For example, lacking observational data, seismologists must rely on simplified theoretical and numerical models of the earthquake process to estimate near-fault ground motion, especially for earthquakes as large as magnitude 7 and 8. Because such models have not been adequately tested against data, their reliability is unknown. Engineers lack detailed information about failure processes in structures during an earthquake. Although many structures have been instrumented to measure their response to an earthquake, few records have been obtained from buildings that actually sustained significant structural damage and few structures are properly instrumented to measure all the modes of deformation that are likely to contribute to failure. Moreover, the fact that many structures have withstood ground motion more intense than that assumed in their design indicates that conventional methods of design do not take into account important contributions to earthquake resistance by nonstructural elements and by the ability of structural elements to deform inelastically without necessarily causing failure of the structure. It is fortunate when such reserve resistance exists, but better understanding of the sources of reserve strength is needed to determine how large a margin of safety they confer and how they might be affected by changes in construction practices and materials with time. In the next few years we look forward to significant advances in knowledge and to more effective application of what is already known, largely because of substantial funding of research related to seismic engineering by the National Science Foundation . The increasing number of strong-motion seismographs operating in seismically active regions will likely provide for the first time a number of records of damaging levels of ground motion. Significant effort is being directed toward obtaining near-fault records, although many probable sites of future large earthquakes remain inadequately instrumented, especially outside the conterminous United States. New and more complete information on building response and damage mechanisms will be obtained by improved instrumentation of structures and through laboratory investigations of failure in structures and structural elements. Further developments in computer technology and in computer modeling techniques will permit more realistic simulations of the seismic response of soils and structures that take into account their inelastic behavior and their strain-dependent properties. Earthquake design codes will continually be revised to better utilize existing knowledge concerning the nature of strong ground motion and the dynamic behavior of buildings during earthquakes and to incorporate new knowledge and also experiences gained from future earthquakes. We believe that application of new knowledge, improvements in earthquake-resistant design and construction, and remedial strengthening or replacement of weak existing structures can significantly reduce our current level of exposure to earthquake hazards.

Science↗

Three-dimensional imaging, change detection, and stability assessment during the centerline trench levee seepage experiment using terrestrial light detection and ranging technology, Twitchell Island, California, 2012

A full scale field seepage test was conducted on a north-south trending levee segment of a now bypassed old meander belt on Twitchell Island, California, to understand the effects of live and decaying root systems on levee seepage and slope stability. The field test in May 2012 was centered on a north-south trench with two segments: a shorter control segment and a longer seepage test segment. The complete length of the trench area measured 40.4 meters (m) near the levee centerline with mature trees located on the waterside and landside of the levee flanks. The levee was instrumented with piezometers and tensiometers to measure positive and negative porewater pressures across the levee after the trench was flooded with water and held at a constant hydraulic head during the seepage test—the results from this component of the experiment are not discussed in this report. We collected more than one billion three-dimensional light detection and ranging (lidar) data points before, during, and after the centerline seepage test to assess centimeter-scale stability of the two trees and the levee crown. During the seepage test, the waterside tree toppled (rotated 20.7 degrees) into the water. The landside tree rotated away from the levee by 5 centimeters (cm) at a height of 2 m on the tree. The paved surface of the levee crown had three regions that showed subsidence on the waterside of the trench—discussed as the northern, central, and southern features. The northern feature is an elongate region that subsided 2.1 cm over an area with an average width of 1.35 m that extends 15.8 m parallel to the trench from the northern end of the trench to just north of the trench midpoint, and is associated with a crack 1 cm in height that formed during the seepage test on the trench wall. The central subsidence feature is a semicircular region on the waterside of the trench that subsided by as much as 6.2 cm over an area 3.4 m wide and 11.2 m long. The southern feature is an elongate region that has a maximum subsidence of 3.5 cm over an area 0.75 m wide and 8.1 m long and is associated with a number of small fractures in the pavement that are predominately north-south-trending and parallel to the trench. We determined that there was no significant motion of the levee flank during the last week of the seepage test. We also determined biomorphic parameters for the landside tree, such as the 3D positioning on the levee, tree height, levee parallel/perpendicular cross sectional area, and canopy centroid. These biomorphic parameters were requested to support a University of California Berkeley team studying seepage and stability on the levee. A gridded, 2-cm bare-earth digital elevation model of the levee crown and the landside levee flank from the final terrestrial lidar (T-Lidar) survey provided detailed topographic data for future assessment. Because the T-Lidar was not integrated into the project design, other than an initial courtesy dataset to help characterize the levee surface, our ability to contribute to the overall science goals of the seepage test was limited. Therefore, our analysis focused on developing data collection and processing methodology necessary to align ultra high-resolution T-Lidar data (with an average spot spacing 2–3 millimeters on the levee crown) from several instrument setup locations to detect, measure, and characterize dynamic centimeter-scale deformation and surface changes during the seepage test.

California↗

Sorption of selected organic compounds from water to a peat soil and its humic-acid and humin fractions: Potential sources of the sorption nonlinearity

The sorption isotherms of ethylene dibromide (EDB), diuron (DUN), and 3,5-dichlorophenol (DCP) from water on the humic acid and humin fractions of a peat soil and on the humic-acid of a muck soil have been measured. The data were compared with those of the solutes with the whole peat from which the humic-acid (HA) and humin (HM) fractions were derived and on which the sorption of the solutes exhibited varying extents of nonlinear capacities at low relative concentrations (C(e)/S(w)). The HA fraction as prepared by the density-fractionated method is relatively pure and presumably free of high- surface-area carbonaceous material (HSACM) that is considered to be responsible for the observed nonlinear sorption for nonpolar solutes (e.g., EDB) on the peat; conversely, the base-insoluble HM fraction as prepared is presumed to be enriched with HSACM, as manifested by the greatly higher BET- (N2) surface area than that of the whole peat. The sorption of EDB on HA exhibits no visible nonlinear effect, whereas the sorption on HM shows an enhanced nonlinearity over that on the whole peat. The sorption of polar DUN and DCP on HA and HM display nonlinear effects comparable with those on the whole peat; the effects are much more significant than those with nonpolar EDB. These results conform to the hypothesis that adsorption onto a small amount of strongly adsorbing HSACM is largely responsible for the nonlinear sorption of nonpolar solutes on soils and that additional specific interactions with the active groups of soil organic matter are responsible for the generally higher nonlinear sorption of the polar solutes.

Environmental Science & Technology↗