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USGS1998

The Loma Prieta, California, earthquake of October 17, 1989 - Earth structures and engineering characterization of ground motion

This chapter contains two papers that summarize the performance of engineered earth structures, dams and stabilized excavations in soil, and two papers that characterize for engineering purposes the attenuation of ground motion with distance during the Loma Prieta earthquake. Documenting the field performance of engineered structures and confirming empirically based predictions of ground motion are critical for safe and cost effective seismic design of future structures as well as the retrofitting of existing ones.

California↗

USGS2005

Studies of the Chesapeake Bay impact structure - Introduction and discussion

The late Eocene Chesapeake Bay impact structure on the Atlantic margin of Virginia is the largest known impact crater in the United States, and it may be the Earth's best preserved example of a large impact crater that formed on a predominantly siliciclastic continental shelf. The 85-kilometer-wide (53-milewide) crater also coincides with a region of saline ground water. It has a profound influence on ground-water quality and flow in an area of urban growth. The USGS-NASA Langley corehole at Hampton , Va., is the first in a series of new coreholes being drilled in the crater, and it is the first corehole to penetrate the entire crater-fill section and uppermost crystalline basement rock. The Langley corehole is located in the southwestern part of the crater's annular trough. A comprehensive effort to understand the crater's materials, architecture, geologic history , and formative processes, as well as its influence on ground water, includes the drilling of coreholes accompanied by high-resolution seismic-reflection and seismic-refraction surveys, audio-magnetotelluric surveys, and related multidisciplinary research. The studies of the core presented in this volume provide detailed information on the outer part of the crater, including the crystalline basement , the overlying impact -modified and impact -generated sediments (physical geology, paleontology, shocked minerals, and crystalline ejecta), and the upper Eocene to Quaternary postimpact sedimentary section (stratigraphy, paleontology, and paleoenvironments). The USGS-NASA Langley corehole has a total depth below land surface of 635.1 meters (m; 2,083.8 feet (ft)). The deepest unit in the corehole is the Neoproterozoic Langley Granite. The top of this granite at 626.3 m (2,054.7 ft) depth is overlain by 390.6 m (1,281.6 ft) of impact -modified and impact -generated siliciclastic sediments. These crater-fill materials are preserved beneath a 235.6-m-thick (773.12-ft-thick) blanket of postimpact sediments. A high-resolution seismic-reflection and seismic-refraction profile that crosses the Langley drill site is tied to the core by borehole geophysical logs, and it reveals the details of extensional collapse structures in the western annular trough. Electrical cross sections based on audio-magnetotelluric (AMT) soundings image a nearly vertical zone of high resistivity at the outer margin of the annular trough, possibly indicating fresh ground water at that location, and they show impedance trends that match the curvature of the structure . They also image the subsurface contact between conductive sediments and resistive crystalline basement , showing that the depth to crystalline basement is relatively constant in the western part of the annular trough. Chemical and isotopic data indicate that saline ground water of the Virginia inland saltwater wedge or bulge is a mixture of freshwater and seawater, and evidence for a mixing zone at the crater's outer margin supports the concept of differential flushing of residual seawater to create the bulge. Ground-water brine in the central part of the crater was produced by evaporation, and brine production from the heat of the impact is at least theoretically possible.

Chesapeake Bay↗

USGS2023

Roles of regional structures and country-rock facies in defining mineral belts in central Idaho mineral province with detail for Yellow Pine and Thunder Mountain mining districts

The central Idaho metallogenic province hosts numerous mineral deposit types. These include Late Cretaceous precious-polymetallic vein deposits, amagmatic Paleocene–Eocene breccia-hosted gold-tungsten-antimony deposits, and Eocene mercury deposits in metasedimentary roof pendants and in Late Cretaceous granitoids. Hot-springs gold deposits in Eocene volcanic rocks are also included in the central Idaho province. New sensitive high mass-resolution ion microprobe (SHRIMP) uranium-lead (U-Pb) ages for igneous rocks and for detrital zircon analyses of metasedimentary rocks along with geologic mapping clarify the geologic framework of the mineral deposits. This framework includes (1) structural controls for regional distribution of mining districts, (2) progressive structural development of individual districts, (3) regional sedimentary facies and their control of metals associations resulting in regional belts, and (4) influences of the several regional magmatic events. In central Idaho, 15 mining districts form two clusters that are grouped about a 200-kilometer (km) long system of normal faults. The northwestern cluster is in the regional hanging wall west of large, west-side-down faults, and the mineral deposits are located along smaller faults and fractures that cut the regional hanging wall. The southeastern cluster is in the regional hanging wall east of a linked large east-side-down fault and along and controlled by related hanging wall faults. At the southern extent of the regional fault system, the Yellow Pine-Thunder Mountain districts span a nearly 24-km-wide, east-tilted crustal block of normal-fault dominoes, exposing original crustal depths from 5 to 10 km deep on the west in the Late Cretaceous to shallow-surface depths on the east in the Eocene. Ore deposition in the northwestern district cluster was primarily Late Cretaceous and related to Idaho batholith plutons with only a single deposit related to a small Eocene intrusion; in the southeastern cluster, most deposits were initiated in the Late Cretaceous but with varying manifestations of overprinted Eocene mineralization activity. In the Yellow Pine-Thunder Mountain districts at the southern extent of the southern cluster, several mineralizing pulses occurred during hanging-wall collapse, such that (1) early deposits were multiply overprinted and (2) deposit depths, ages, and structural characteristics change progressively eastward. Originally deep-seated western Yellow Pine district deposits are Late Cretaceous viscoplastic mesothermal veins overprinted by Paleocene and Eocene breccia-hosted epithermal deposits. Central Yellow Pine district deposits contain early deeper vein systems but are primarily Paleocene and Eocene breccia-hosted epithermal deposits in Late Cretaceous plutonic rocks and Proterozoic–Paleozoic roof pendant rocks. Eastern district deposits are Eocene hot-springs-related deposits in the roof pendant. Thunder Mountain deposits farthest east are near-surface hot-springs deposits in Eocene volcanic and volcaniclastic rocks that overlie buried Cretaceous igneous and older roof pendant rocks. The mining district clusters are sited across several northwest-striking paleostratigraphic belts that are exposed in roof pendants and are offset by the regional normal fault system. A northeastern belt is Mesoproterozoic strata associated with gold-silver-copper±cobalt deposits. A central belt of Neoproterozoic rocks is not associated with mineral deposits in the central Idaho mineral province. A southwestern belt composed of probable Paleozoic deep-water miogeoclinal slope rocks and late Paleozoic epicratonic basinal rocks is thin and narrowly exposed but associated with gold-silver-antimony-tungsten±mercury deposits. These metasedimentary rocks (and their metal associations) are parts of regional mineral belts in which metal endowments are related to particular sedimentary facies belts and their Cretaceous thrust-fault juxtaposition and where these features have proximity to Late Cretaceous or Eocene igneous rocks. Offset and preservation or erosional stripping of these facies belts, thrust plates, igneous settings, and the associated regional mineral belts were controlled by the sense and magnitude of displacements across the regional normal-fault system.

Idaho↗

USGS2014

Bedrock geologic and structural map through the western Candor Colles region of Mars

The Candor Colles are a population of low, conical hills along the southeast flank of Ceti Mensa, in west Candor Chasma, within the Valles Marineris system of Mars (fig. 1). Ceti Mensa and the adjacent Candor Mensa are mounds of layered sedimentary deposits and are the most prominent landforms within west Candor Chasma. Prior to the arrival of the Mars Reconnaissance Orbiter (MRO) in orbit around Mars in 2006 (Zurek and Smrekar, 2007), geologic maps of the area utilized the relatively low resolution Viking Orbiter photomosaics (20–150 m/pixel). Geologic maps covering west Candor Chasma were created at scales of 1:15,000,000 for the western equatorial region of Mars (Scott and Tanaka, 1986), 1:2,000,000 for the Valles Marineris region (Witbeck and others, 1991), and 1:500,000 for the far eastern part of west Candor Chasma (Mars Transverse Mercator quadrangle–05072; Lucchitta, 1999). Previous structural mapping in west Candor Chasma at scales of less than 1:24,000 (Okubo and others, 2008; Okubo, 2010) employed digital terrain models (DTMs), with 1-m post spacings, derived from stereo MRO High Resolution Imaging Science Experiment (HiRISE) imagery (McEwen and others, 2010) and focused on examining the relative timing between deposition of the youngest unit of the layered deposits in this area (unit Avme of Witbeck and others, 1991) relative to regional faulting related to chasma formation. These previous mapping efforts on the southwest flank of Ceti Mensa demonstrated that unit Avme is not deformed by faults attributed to formation of the chasma. Studies of other layered deposits (primarily unit Hvl, but also including units Avme, Avsl, Avsd, and Avfs; Witbeck and others, 1991) exposed along the southeast flank of Ceti Mensa using a High-Resolution Stereo Camera (HRSC) digital terrain model (DTM) (50 m/pixel) refined the local stratigraphy and revealed evidence for syntectonic deposition of these deposits (Fueten and others, 2006, 2008; Jaumann and others, 2007; Birnie and others, 2012). Layered deposits such as those that constitute Ceti Mensa are widespread throughout the interior regions of Valles Marineris (Witbeck and others, 1991). These sedimentary deposits have been variously interpreted as eolian sediments (Nedell and others, 1987), hyaloclastic debris (Chapman and Tanaka, 2001; Komatsu and others, 2004), lacustrine or fluvial sediment (Dromart and others, 2007; Mangold and others, 2008; Metz and others, 2009), pyroclastic deposits (Hynek and others, 2003), evaporites (Mangold and others, 2008; Andrews-Hanna and others, 2010), or various combinations thereof. Recent analysis of data from the MRO Compact Reconnaissance Imaging Spectrometer for Mars (CRISM) shows that these sediments consist primarily of hydrated sulfates (Murchie and others, 2009a,b). Further, hydrologic modeling indicates that spring-fed lakes likely occurred within the chasma (Andrews-Hanna and others, 2010). These recent findings point to a scenario in which the layered deposits accumulated as sequences of evaporites precipitating in hypersaline lakes, with contemporaneous trapping of eolian dust and sand, diagenesis, and iron-cycling, interspersed with periods of eolian and fluvial erosion (Murchie and others, 2009a). Water vapor released from these lakes may have also driven localized precipitation of snow and accumulation of layered deposits on the adjacent plateaus (Kite and others, 2011a,b). This scenario is in contrast to recent alternative interpretations that the layered deposits formed within the chasma through weathering of dust-rich ice deposits (Niles and Michalski, 2009; Michalski and Niles, 2012). The structure and geology of the layered deposits in the Candor Colles region corresponding to units Avfs, Avme, and Hvl of Witbeck and others (1991) are reevaluated in this 1:18,000-scale map. The objectives herein are to gather high-resolution structural measurements to (1) refine the previous unit boundaries in this area established by Witbeck and others (1991), (2) revise the local stratigraphy where necessary, (3) characterize bed forms to help constrain depositional processes, and (4) determine the styles and extent of deformation to better inform reconstructions of the local post-depositional geologic history.

Scientific Investigations Map↗

USGS2004

Preliminary investigation of structural controls of ground-water movement in Pipe Spring National Monument, Arizona

Pipe Spring National Monument, near the border of Arizona and Utah, includes several low-discharge springs that are the primary natural features of the monument. The National Park Service is concerned about the declines in spring discharge. Seismic-refraction and frequency-domain electromagnetic-induction methods were employed in an attempt to better understand the relation between spring discharge and geologic structure. The particular method used for the seismic-refraction surveys was unable to resolve structural features in the monument. Electromagnetic surveys delineated differences in apparent conductivity of the shallow subsurface deposits. The differences are attributable to differences in saturation, lithology, and structure of these deposits.

Scientific Investigations Report↗

USGS2009

Connections Among the Spatial and Temporal Structures in Tidal Currents, Internal Bores, and Surficial Sediment Distributions Over the Shelf off Palos Verdes, California

The topography of the Continental Shelf in the central portion of the Southern California Bight has rapid variations over relatively small spatial scales. The width of the shelf off the Palos Verdes peninsula, just northwest of Los Angeles, California, is only 1 to 3 km. About 7 km southeast of the peninsula, the shelf within San Pedro Bay widens to about 20 km. In 2000, the Los Angeles County Sanitation District began deploying a dense array of moorings in this complex region of the central Southern California Bight to monitor local circulation patterns. Moorings were deployed at 13 sites on the Palos Verdes shelf and within the northwestern portion of San Pedro Bay. At each site, a mooring supported a string of thermistors and an adjacent bottom platform housed an Acoustic Doppler Current Profiler. These instruments collected vertical profiles of current and temperature data continuously for one to two years. The variable bathymetry in the region causes rapid changes in the amplitudes and spatial structures of barotropic tidal currents, internal tidal currents, and in the associated nonlinear baroclinic currents that occur at approximate tidal frequencies. The largest barotropic tidal constituent is M2, the principal semidiurnal tide. The amplitude of this tidal current changes over fairly short along-shelf length scales. Tidal-current amplitudes are largest in the transition region between the two shelves; they increase from about 5 cm/s over the northern San Pedro shelf to nearly 10 cm/s on the southern portion of the Palos Verdes Shelf. Tidal-current amplitudes are then reduced to less than 2 cm/s over the very narrow section of the northern Palos Verdes shelf that lies just 6 km upcoast of the southern sites. Models suggest that the amplitude of the barotropic M2 tidal currents, which propagate toward the northwest primarily as a Kelvin wave, is adjusting to the short topographic length scales in the region. Semidiurnal sea-level oscillations are, as expected, independent of these topographic variations; they have a uniform amplitude and phase structure over the entire region. Because the cross-shelf angle of the seabed over most of the Palos Verdes shelf is 1 to 3 degrees, which is critical for the local generation and/or enhancement of nonlinear characteristics in semidiurnal internal tides, some internal tidal-current events have strong asymmetric current oscillations that are enhanced near the seabed. Near-bottom currents in these events are directed primarily offshore with amplitudes that exceed 30 cm/s. The spatial patterns in these energetic near-bottom currents have fairly short-length scales. They are largest over the inner shelf and in the transition region between the Palos Verdes and San Pedro shelves. This spatial pattern is similar to that found in the barotropic tidal currents. Because these baroclinic currents have an approximate tidal frequency, an asymmetric vertical structure, and a somewhat stable phase, they can produce a non-zero depth-mean flow for periods of a few months. These baroclinic currents can interact with the barotropic tidal current and cause an apparent increase (or decrease) in the estimated barotropic tidal-current amplitude. The apparent amplitude of the barotropic tidal current may change by 30 to 80 percent or more in a current record that is less than three months long. The currents and surficial sediments in this region are in dynamic equilibrium in that the spatial patterns in bottom stresses generated by near-bed currents from surface tides, internal tides, and internal bores partly control the spatial patterns in the local sediments. Coarser sediments are found in the regions with enhanced bottom stresses (that is, over the inner shelf and in the region between the Palos Verdes and San Pedro shelves). Finer sediments are found over the northwestern portion of the Palos Verdes shelf, where near-bottom currents are relatively weak. The nonlinear asymmetries in the i

Scientific Investigations Report↗

USGS2013

Investigation of the structure and lithology of bedrock concealed by basin fill, using ground-based magnetic-field-profile data acquired in the San Rafael Basin, southeastern Arizona

Data on the Earth’s total-intensity magnetic field acquired near ground level and at measurement intervals as small as 1 m include information on the spatial distribution of nearsurface magnetic dipoles that in many cases are unique to a specific lithology. Such spatial information is expressed in the texture (physical appearance or characteristics) of the data at scales of hundreds of meters to kilometers. These magnetic textures are characterized by several descriptive statistics, their power spectrum, and their multifractal spectrum. On the basis of a graphical comparison and textural characterization, ground-based magnetic-field profile data can be used to estimate bedrock lithology concealed by as much as 100 m of basin fill in some cases, information that is especially important in assessing and exploring for concealed mineral deposits. I demonstrate that multifractal spectra of ground-based magnetic-field-profile data can be used to differentiate exposed lithologies and that the shape and position of the multifractal spectrum of the ground-based magnetic-field-profile of concealed lithologies can be matched to the upward-continued multifractal spectrum of an exposed lithology to help distinguish the concealed lithology. In addition, ground-based magnetic-field-profile data also detect minute differences in the magnetic susceptibility of rocks over small horizontal and vertical distances and so can be used for precise modeling of bedrock geometry and structure, even when that bedrock is concealed by 100 m or more of nonmagnetic basin fill. Such data contain valuable geologic information on the bedrock concealed by basin fill that may not be so visible in aeromagnetic data, including areas of hydrothermal alteration, faults, and other bedrock structures. Interpretation of these data in the San Rafael Basin, southeastern Arizona, has yielded results for estimating concealed lithologies, concealed structural geology, and a concealed potential mineral-resource target.

Arizona↗

USGS2014

Statistics for stochastic modeling of volume reduction, hydrograph extension, and water-quality treatment by structural stormwater runoff best management practices (BMPs)

The U.S. Geological Survey (USGS) developed the Stochastic Empirical Loading and Dilution Model (SELDM) in cooperation with the Federal Highway Administration (FHWA) to indicate the risk for stormwater concentrations, flows, and loads to be above user-selected water-quality goals and the potential effectiveness of mitigation measures to reduce such risks. SELDM models the potential effect of mitigation measures by using Monte Carlo methods with statistics that approximate the net effects of structural and nonstructural best management practices (BMPs). In this report, structural BMPs are defined as the components of the drainage pathway between the source of runoff and a stormwater discharge location that affect the volume, timing, or quality of runoff. SELDM uses a simple stochastic statistical model of BMP performance to develop planning-level estimates of runoff-event characteristics. This statistical approach can be used to represent a single BMP or an assemblage of BMPs. The SELDM BMP-treatment module has provisions for stochastic modeling of three stormwater treatments: volume reduction, hydrograph extension, and water-quality treatment. In SELDM, these three treatment variables are modeled by using the trapezoidal distribution and the rank correlation with the associated highway-runoff variables. This report describes methods for calculating the trapezoidal-distribution statistics and rank correlation coefficients for stochastic modeling of volume reduction, hydrograph extension, and water-quality treatment by structural stormwater BMPs and provides the calculated values for these variables. This report also provides robust methods for estimating the minimum irreducible concentration (MIC), which is the lowest expected effluent concentration from a particular BMP site or a class of BMPs. These statistics are different from the statistics commonly used to characterize or compare BMPs. They are designed to provide a stochastic transfer function to approximate the quantity, duration, and quality of BMP effluent given the associated inflow values for a population of storm events. A database application and several spreadsheet tools are included in the digital media accompanying this report for further documentation of methods and for future use. In this study, analyses were done with data extracted from a modified copy of the January 2012 version of International Stormwater Best Management Practices Database, designated herein as the January 2012a version. Statistics for volume reduction, hydrograph extension, and water-quality treatment were developed with selected data. Sufficient data were available to estimate statistics for 5 to 10 BMP categories by using data from 40 to more than 165 monitoring sites. Water-quality treatment statistics were developed for 13 runoff-quality constituents commonly measured in highway and urban runoff studies including turbidity, sediment and solids; nutrients; total metals; organic carbon; and fecal coliforms. The medians of the best-fit statistics for each category were selected to construct generalized cumulative distribution functions for the three treatment variables. For volume reduction and hydrograph extension, interpretation of available data indicates that selection of a Spearman’s rho value that is the average of the median and maximum values for the BMP category may help generate realistic simulation results in SELDM. The median rho value may be selected to help generate realistic simulation results for water-quality treatment variables. MIC statistics were developed for 12 runoff-quality constituents commonly measured in highway and urban runoff studies by using data from 11 BMP categories and more than 167 monitoring sites. Four statistical techniques were applied for estimating MIC values with monitoring data from each site. These techniques produce a range of lower-bound estimates for each site. Four MIC estimators are proposed as alternatives for selecting a value from among the estimates from multiple sites. Correlation analysis indicates that the MIC estimates from multiple sites were weakly correlated with the geometric mean of inflow values, which indicates that there may be a qualitative or semiquantitative link between the inflow quality and the MIC. Correlations probably are weak because the MIC is influenced by the inflow water quality and the capability of each individual BMP site to reduce inflow concentrations.

Scientific Investigations Report↗

USGS2022

A structured decision-making framework for managing cyanobacterial harmful algal blooms in New York State parks

Cyanobacteria are increasingly a global water-quality concern because of the potential for these organisms to develop into potentially harmful blooms that affect ecological, economic, and public health. Cyanobacterial harmful algal blooms (CyanoHABs) can lead to a decrease in water quality and affect many of the recreational and ecological benefits of parks that include lakes. The New York State Office of Parks, Recreation and Historic Preservation (OPRHP) is a State agency within the New York State Executive Department charged with the operation of State parks and historic sites. Many New York State parks include lakes or other freshwater bodies, which can be susceptible to CyanoHABs. The OPRHP faces difficult decisions regarding prevention of and response to CyanoHABs. The U.S. Geological Survey partnered with the OPRHP and the New York State Department of Environmental Conservation to develop a structured decision-making template for managing CyanoHABs in OPRHP parks. Two parks, Moreau Lake State Park and Rockland Lake State Park, served as case studies to motivate and test the template. This report describes how the principles of structured decision making can be used to navigate the challenges associated with managing CyanoHABs in OPRHP parks. Management objectives and strategies for CyanoHABs in parks are described, strategies to evaluate consequences and manage tradeoffs are discussed, and potential challenges to the implementation of preferred alternatives are considered. General guidance is provided so the OPRHP can undertake the structured decision-making process for CyanoHABs in any of its parks. In addition, this report represents the first effort to create a strategy for applying decision analysis tools to the complex natural resource challenge of CyanoHAB mitigation and management. The case studies and template are intended to serve as an example that natural resource managers faced with CyanoHABs challenges can use to inform their decision-making processes.

New York↗

USGS2002

Characterization and analysis of temporal and spatial variations in habitat and macroinvertebrate community structure, Fountain Creek basin, Colorado Springs and vicinity, Colorado, 1998-2001

The Fountain Creek Basin in and around Colorado Springs, Colorado, is affected by various land- and water-use activities. Biological, hydrological, water-quality, and land-use data were collected at 10 sites in the Fountain Creek Basin from April 1998 through April 2001 to provide a baseline characterization of macroinvertebrate communities and habitat conditions for comparison in subsequent studies; and to assess variation in macroinvertebrate community structure relative to habitat quality. Analysis of variance results indicated that instream and riparian variables were not affected by season, but significant differences were found among sites. Nine metrics were used to describe and evaluate macroinvertebrate community structure. Statistical analysis indicated that for six of the nine metrics, significant variability occurred between spring and fall seasons for 60 percent of the sites. Cluster analysis (unweighted pair group method average) using macroinvertebrate presence-absence data showed a well-defined separation between spring and fall samples. Six of the nine metrics had significant spatial variation. Cluster analysis using Sorenson?s Coefficient of Community values computed from macroinvertebrate density (number of organisms per square meter) data showed that macroinvertebrate community structure was more similar among tributary sites than main-stem sites. Canonical correspondence analysis identified a substrate particle-size gradient from site-specific species-abundance data and environmental correlates that decreased the 10 sites to 5 site clusters and their associated taxa.

Colorado↗

USGS1986

Potentiometric-surface map of the Wyodak-Anderson coal bed, Powder River structural basin, Wyoming, 1973-84

Previous water level maps of shallow aquifers in the Powder River structural basin in Wyoming were based on water levels from wells completed in different stratigraphic intervals within thick sequences of sedimentary rocks. A potentiometric surface using water levels from a single aquifer had never been mapped throughout the basin. The sandstone aquifers in the Fort Union Formation of Paleocene age and the Wasatch Formation of Eocene age are discontinuous and lenticular, and do not extend even short distances. Coal aquifers are more continuous and the Wyodak-Anderson coal bed, in the Fort Union Formation, has been mapped in much of the Powder River structural basin in Wyoming. Water level altitudes in the Wyodak-Anderson coal bed and other stratigraphically equivalent coal beds were mapped to determine if they represent a continuous potentiometric surface in the Powder River structural basin. The potentiometric surface, except in the vicinity of the Wyodak mine east of Gillette, represents a premining condition as it was based on water level measurements made during 1973-84 that were not significantly affected by mining. The map was prepared in cooperation with the U.S. Bureau of Land Management. (Lantz-PTT)

Wyoming↗

USGS1991

Characterization of structural proteins of hirame rhabdovirus, HRV

Structural proteins of hirame rhabdovirus (HRV) were analyzed by SDS-polyacrylarnide gel electrophoresis, western blotting, 2-dimensional gel electrophoresis, and Triton X-100 treatment. Purified HRV virions were composed of: polymerase (L), glycoprotein (G), nucleoprotein (N), and 2 matrix proteins (M1 and M2). Based upon their relative mobilities, the estimated molecular weights of the proteins were: L, 156 KDa; G, 68 KDa; N, 46.4 KDa; M1, 26.4 KDa; and M2, 19.9 KDa. The electrophorehc pattern formed by the structural proteins of HRV was clearly different from that formed by pike fry rhabdovirus, spring viremia of carp virus, eel virus of America, and eel virus European X which belong to the Vesiculovirus genus; however, it resembled the pattern formed by structural proteins of viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV) which are members of the Lyssavirus genus. Among HRV, IHNV, and VHSV, differences were observed in the relative mobilities of the G, N, M1, and M2 proteins. Western blot analysis revealed that the G. N, and M2 proteins of HRV shared antigenic determinants with IHNV and VHSV, but not with any of the 4 fish vesiculoviruses tested. Cross-reactions between the M1 proteins of HRV, IHNV, or VHSV were not detected in this assay. Two-dimensional gel electrophoresis was used to show that HRV differed from IHNV or VHSV in the isoelectric point (PI) of the M1 and M2 proteins. In this system, 2 forms of the M1 protein of HRV and IHNV were observed.These subspecies of M1 had the same relative mobility but different p1 values. Treatment of purified virions with 2% Triton X-100 in Tris buffer containing NaCl removed the G, M1, and M2 proteins of IHNV, but HRV virions were more stable under these conditions.

Diseases of Aquatic Organisms↗

USGS2025

New insights reveal a temporally distinct two-stock genetic structure for Suwannee River Gulf sturgeon

Understanding population genetic structure and patterns of gene flow is important for effective decision making and the preservation of genetic diversity, especially when managing protected species. Historically, Gulf sturgeon have been managed by river system, with early evidence supporting spatially distinct genetic structure across 7 natal populations. However, an increasing number of studies recognize that some rivers possess 2 genetically distinct sturgeon stocks residing within the same drainage. This, combined with evidence of both spring and fall spawning events within these drainages, suggests the distinction between these stocks may be temporally mediated, as similarly exhibited by the closely related Atlantic sturgeon. We analyzed the movements of 136 Gulf sturgeon within the Suwannee River, Florida, USA, between 2008 and 2022 using acoustic telemetry. We assessed migratory patterns and presence within known spawning habitat to categorize individuals as exhibiting either spring, fall, or unknown spawning behaviors each year. These individuals were also genotyped for 14 microsatellite loci to assign them to a genetic group. Our results reinforce previous work suggesting the presence of 2 genetically distinct stocks in the Suwannee River and indicate this genetic structure is driven by fidelity to temporally distinct spawning events, with one stock spawning in the spring and the other in fall. The delineation of these 2 sympatric stocks, historically managed as one, has management implications for the recovery of this species.

Florida, Georgia↗

USGS2011

Quantifying seascape structure: Extending terrestrial spatial pattern metrics to the marine realm

Spatial pattern metrics have routinely been applied to characterize and quantify structural features of terrestrial landscapes and have demonstrated great utility in landscape ecology and conservation planning. The important role of spatial structure in ecology and management is now commonly recognized, and recent advances in marine remote sensing technology have facilitated the application of spatial pattern metrics to the marine environment. However, it is not yet clear whether concepts, metrics, and statistical techniques developed for terrestrial ecosystems are relevant for marine species and seascapes. To address this gap in our knowledge, we reviewed, synthesized, and evaluated the utility and application of spatial pattern metrics in the marine science literature over the past 30 yr (1980 to 2010). In total, 23 studies characterized seascape structure, of which 17 quantified spatial patterns using a 2-dimensional patch-mosaic model and 5 used a continuously varying 3-dimensional surface model. Most seascape studies followed terrestrial-based studies in their search for ecological patterns and applied or modified existing metrics. Only 1 truly unique metric was found (hydrodynamic aperture applied to Pacific atolls). While there are still relatively few studies using spatial pattern metrics in the marine environment, they have suffered from similar misuse as reported for terrestrial studies, such as the lack of a priori considerations or the problem of collinearity between metrics. Spatial pattern metrics offer great potential for ecological research and environmental management in marine systems, and future studies should focus on (1) the dynamic boundary between the land and sea; (2) quantifying 3-dimensional spatial patterns; and (3) assessing and monitoring seascape change.

Marine Ecology Progress Series↗

USGS2004

Hydrologic aspects of marsh ponds during winter on the Gulf Coast Chenier Plain, USA: Effects of structural marsh management

The hydrology of marsh ponds influences aquatic invertebrate and waterbird communities. Hydrologic variables in marsh ponds of the Gulf Coast Chenier Plain are potentially affected by structural marsh management (SMM: levees, water control structures and impoundments) that has been implemented since the 1950s. Assuming that SMM restricts tidal flows and drainage of rainwater, we predicted that SMM would increase water depth, and concomitantly decrease salinity and transparency in impounded marsh ponds. We also predicted that SMM would increase seasonal variability in water depth in impounded marsh ponds because of the potential incapacity of water control structures to cope with large flooding events. In addition, we predicted that SMM would decrease spatial variability in water depth. Finally, we predicted that ponds of impounded freshwater (IF), oligohaline (IO), and mesohaline (IM) marshes would be similar in water depth, temperature, dissolved oxygen ( O 2 ), and transparency. Using a priori multivariate analysis of variance (MANOVA) contrast, we tested these predictions by comparing hydrologic variables within ponds of impounded and unimpounded marshes during winters 1997-1998 to 1999-2000 on Rockefeller State Wildlife Refuge, near Grand Chenier, Louisiana. Specifically, we compared hydrologic variables (1) between IM and unimpounded mesohaline marsh ponds (UM); and (2) among IF, IO, and IM marshes ponds. As predicted, water depth was higher and salinity and O 2 were lower in IM than in UM marsh ponds. However, temperature and transparency did not differ between IM and UM marsh ponds. Water depth varied more among months in IM marsh ponds than within those of UM marshes, and variances among and within ponds were lower in IM than UM marshes. Finally, all hydrologic variables, except salinity, were similar among IF, IO, and IM marsh ponds. Hydrologic changes within marsh ponds due to SMM should (1) promote benthic invertebrate taxa that tolerate low levels of O 2 and salinity; (2) deter waterbird species that cannot cope with increased water levels; and (3) reduce waterbird species diversity by decreasing spatial variability in water depth among and within marsh ponds.

Louisiana↗

USGS2015

How spatio-temporal habitat connectivity affects amphibian genetic structure

Heterogeneous landscapes and fluctuating environmental conditions can affect species dispersal, population genetics, and genetic structure, yet understanding how biotic and abiotic factors affect population dynamics in a fluctuating environment is critical for species management. We evaluated how spatio-temporal habitat connectivity influences dispersal and genetic structure in a population of boreal chorus frogs ( Pseudacris maculata ) using a landscape genetics approach. We developed gravity models to assess the contribution of various factors to the observed genetic distance as a measure of functional connectivity. We selected (a) wetland (within-site) and (b) landscape matrix (between-site) characteristics; and (c) wetland connectivity metrics using a unique methodology. Specifically, we developed three networks that quantify wetland connectivity based on: (i) P. maculata dispersal ability, (ii) temporal variation in wetland quality, and (iii) contribution of wetland stepping-stones to frog dispersal. We examined 18 wetlands in Colorado, and quantified 12 microsatellite loci from 322 individual frogs. We found that genetic connectivity was related to topographic complexity, within- and between-wetland differences in moisture, and wetland functional connectivity as contributed by stepping-stone wetlands. Our results highlight the role that dynamic environmental factors have on dispersal-limited species and illustrate how complex asynchronous interactions contribute to the structure of spatially-explicit metapopulations.

Colorado↗

USGS2021

Oil and gas wastewater components alter streambed microbial community structure and function

The widespread application of directional drilling and hydraulic fracturing technologies expanded oil and gas (OG) development to previously inaccessible resources. A single OG well can generate millions of liters of wastewater, which is a mixture of brine produced from the fractured formations and injected hydraulic fracturing fluids (HFFs). With thousands of wells completed each year, safe management of OG wastewaters has become a major challenge to the industry and regulators. OG wastewaters are commonly disposed of by underground injection, and previous research showed that surface activities at an Underground Injection Control (UIC) facility in West Virginia affected stream biogeochemistry and sediment microbial communities immediately downstream from the facility. Because microbially driven processes can control the fate and transport of organic and inorganic components of OG wastewater, we designed a series of aerobic microcosm experiments to assess the influence of high total dissolved solids (TDS) and two common HFF additives—the biocide 2,2-dibromo-3-nitrilopropionamide (DBNPA) and ethylene glycol (an anti-scaling additive)—on microbial community structure and function. Microcosms were constructed with sediment collected upstream (background) or downstream (impacted) from the UIC facility in West Virginia. Exposure to elevated TDS resulted in a significant decrease in aerobic respiration, and microbial community analysis following incubation indicated that elevated TDS could be linked to the majority of change in community structure. Over the course of the incubation, the sediment layer in the microcosms became anoxic, and addition of DBNPA was observed to inhibit iron reduction. In general, disruptions to microbial community structure and function were more pronounced in upstream and background sediment microcosms than in impacted sediment microcosms. These results suggest that the microbial community in impacted sediments had adapted following exposure to OG wastewater releases from the site. Our findings demonstrate the potential for releases from an OG wastewater disposal facility to alter microbial communities and biogeochemical processes. We anticipate that these studies will aid in the development of useful models for the potential impact of UIC disposal facilities on adjoining surface water and shallow groundwater.

Frontiers in Microbiology↗

USGS2023

Genetic structure across isolated Virginia populations of the endangered candy darter (Etheostoma osburni)

Candy darter Etheostoma osburni , a federally endangered non-game fish, has been extirpated from most of its historic range in Virginia and now occurs in four isolated populations in the New River drainage. Understanding of population genetic structure will provide insights into the recent natural history of the species and can inform conservation management. Our objectives were to: characterize population genetic structure, estimate and compare effective population sizes ( N e ), and use this information to infer recent population history. Variation at mitochondrial cytochrome b sequences among 150 individuals showed 10 haplotypes separated by 1–14 mutational steps, some shared and some unique to particular populations. Variation at 12 microsatellite loci among 171 individuals showed lower variation in Dismal Creek than in other populations. All populations showed evidence of having experienced a genetic bottleneck and were highly differentiated from one another based on both types of DNA markers. Population genetic structure was related to stream position in regard to the New River, suggesting that populations were once connected. N e estimates for all populations were less than the 500 recommended to maintain evolutionary potential, but most estimates were greater than the 100 needed for use as source populations. Our findings indicate that habitat management to allow expansion of populations, and translocations to exchange genetic material among populations, may be effective tactics to promote conservation of candy darter in Virginia.

North Carolina, Virginia, West Virginia↗