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Evaluating and managing environmental water regimes in a water-scarce and uncertain future

While the number of environmental flows and water science programmes continues to grow across the globe, there remains a critical need to better balance water availability in support of human and ecological needs and to recognise the environment as a legitimate user of water. In water‐stressed areas, this recognition has resulted in friction between water users in the public and private sectors. An opportunity exists for practitioners to be on the forefront of the science determining best practices for supporting environmental water regimes. This Special Issue brings together a collection of environmental flows science and water management papers organised around three major themes: (1) method development and testing; (2) application case studies; and (3) efficacy evaluation. Contents of this Special Issue are intended to foster collaboration and broaden transferability of the information, technical tools, models and methods needed to support environmental water management programmes. The technical sophistication of methods and modelling tools, while important to the advancement of environmental water science, may come at the expense of easily interpretable outcomes that positively influence management decisions. Researchers need to be more proactive in translating the results of advanced modelling methodologies into user‐friendly tools and methods. This will allow stakeholders and water managers to proactively test alternative water allocation scenarios to help address growing human water demands in the face of droughts and changes in climatic patterns. The application of environmental flows science and water management strategies cannot be done in isolation. Implementation involves a complex decision‐making process that integrates ecological, hydrologic and social science across diverse multifaceted governance systems and requires active stakeholder involvement. Scientists and managers must strengthen partnerships at multiple scales to develop sensible science investment strategies so that collective knowledge can be translated into wise environmental water management decisions.

Freshwater Biology↗

The PRISM (Pliocene Palaeoclimate) reconstruction: Time for a paradigm shift

Global palaeoclimate reconstructions have been invaluable to our understanding of the causes and effects of climate change, but single-temperature representations of the oceanic mixed layer for data–model comparisons are outdated, and the time for a paradigm shift in marine palaeoclimate reconstruction is overdue. The new paradigm in marine palaeoclimate reconstruction stems the loss of valuable climate information and instead presents a holistic and nuanced interpretation of multi-dimensional oceanographic processes and responses. A wealth of environmental information is hidden within the US Geological Survey's P liocene R esearch, I nterpretation and S ynoptic M apping (PRISM) marine palaeoclimate reconstruction, and we introduce here a plan to incorporate all valuable climate data into the next generation of PRISM products. Beyond the global approach and focus, we plan to incorporate regional climate dynamics with emphasis on processes, integrating multiple environmental proxies wherever available in order to better characterize the mixed layer, and developing a finer time slice within the Mid-Piacenzian Age of the Pliocene, complemented by underused proxies that offer snapshots into environmental conditions. The result will be a proxy-rich, temporally nested, process-oriented approach in a digital format - a relational database with geographic information system capabilities comprising a three-dimensional grid representing the surface layer, with a plethora of data in each cell.

Philosophical Transactions of the Royal Society A:↗

A climate risk management screening and assessment review for Madagascar’s Country Development Cooperation Strategy

Madagascar, a country rich in natural capital and biodiversity but with high levels of poverty, food insecurity, and population growth, faces a number of development challenges, including obtaining sustained financial support from external sources and building internal capacity to address the poor environmental, health, and socio-economic conditions. Climate change poses an increasing threat to achieving development goals and is usually considered in development plans and project designs. However, there have been numerous challenges in the effective implementation of those plans, particularly in the sustained engagement of the communities to undertake adaptive actions but also due to insufficient scientific information to inform management decisions. To support the United States Agency for International Development (USAID) in the incorporation of Climate Risk Management into their Country Development Cooperation Strategy, we synthesized the best available information on current and future climate change impacts. Climate risks in Madagascar include increasing temperatures, reduced and more variable precipitation, more frequent droughts, more intense cyclones, and rising sea levels. We synthesized the observed and projected impacts of these climatic changes on water resources, agriculture, coastal and terrestrial ecosystems and ecosystem services, fisheries, and human health. Through interviews with stakeholders in Madagascar, we also discerned ongoing and potential climate adaptation and mitigation activities and information gaps (production, access, exploitation, processing, dissemination of information). The information gathered can be used by USAID, NGOs, and other organizations on how to better incorporate climate risks into development strategies and projects in Madagascar.

Project Summary↗

Fish egg injection as an alternative exposure route for early life stage toxicity studies: Description of two unique methods: Chapter 4

In the environment, lipophilic contaminants such as halogenated aromatic hydrocarbons (HAHs, e.g., polychlorinated biphenyls, PCBs) and polycyclic aromatic hydrocarbons (PAHs, e.g., benzo[a]pyrene) readily bioaccumulate in fish, and the bioaccumulation of these lipophilic chemicals by adult fish may have significant consequences on the development and survival of their offspring. Halogenated and polycyclic aromatic hydrocarbons translocate from adult female body stores into eggs during oocyte maturation, and early life stages of fish are often more sensitive than adults to the toxicity of these chemicals. Thus, the presence of persistent, bioaccumulative contaminants in the environment may pose a risk to fish early life stage survival and ultimately reduce recruitment into the adult population. Typically, standard early life stage toxicity studies exposed embryos, larvae, and juveniles to graded concentrations of waterborne toxicants, and dose-response relationships are based on the concentrations of chemicals in the water. However, use of waterborne exposure to assess the toxicity of persistent, bioaccumulative contaminants, such as HAHs and PAHs, has two significant drawbacks. First, uptake of hydrophobic chemicals, such as HAHs and PAHs, into the developing embryo from water is not a significant route of exposure in the environment since concentrations of these chemicals freely dissolved in water are extremely low. Rather, maternal deposition into developing oocytes is the most significant source of these chemicals to the embryo. Second, the dose received by the target tissue, in this case the developing embryo, is the most accurate predictor of the toxic response, and since extrapolation from water concentrations of the chemical to egg concentrations is required, the exact dose received by the embryo can only be estimated, often with large uncertainty. Due to these drawbacks, it is important to develop an alternative exposure method that will directly expose the developing embryo without the need to chronically expose adult fish with subsequent natural deposition of hydrophobic chemicals into the oocytes. Fish egg injection provides this exposure route. Embryos are exposed directly after fertilization with known doses of contaminants, the dose is delivered prior to critical developmental events, and extrapolation of the dose received by the embryo is not needed. We have developed two unique fish egg injection methods as alternative routes of exposure for fish early life stage toxicity studies of lipophilic environmental contaminants. With either method, individual fish eggs are injected with a known dose of chemical. The first approach, a microinjection method, originally developed to assess the developmental toxicity of HAH congeners to early life stages of salmonids, utilizes micro-syringes, 30- gauge stainless steel injection needles, and micro- to nanoliter injection volume. The second approach, a nano-injection method, utilizes glass capillary micropipettes with 2 to 10 µm tips as injection needles, and nano- to picoliter injection volume, allowing injection of nearly any size of fish egg. Both of these egg injection methods allow an investigator to assess the toxicity of lipophilic environmental contaminants to early life stages of fish in a manner that realistically reflects environmental exposure and allows accurate quantitation of the dose to the developing embryo. These injection techniques, however, are not limited to use with only lipophilic chemicals. Since the developmental toxicity of many environmental contaminants ultimately depends on the dose received by the embryo, these egg injection methods could serve as a realistic exposure route in many fish early life stage toxicity studies.

Book chapter↗

Hydrozincite seasonal precipitation at Naracauli (Sardinia – Italy): Hydrochemical factors and morphological features of the biomineralization process

Hydrozincite [Zn 5 (CO 3 ) 2 (OH) 6 ] precipitation from Naracauli waters (SW Sardinia) is, among other things, promoted by a microbial community made up of a filamentous cyanobacterium ( Scytonema sp.) and a microalgae ( Chlorella sp.). Hydrozincite bioprecipitation is responsible for the natural removal of harmful metals, especially Zn, from the stream waters. Thus, hydrozincite could be used to attenuate metal pollution in mining waters. Information on environmental conditions which promote the biomineralization process is fundamental for the development of remediation strategies. This paper aims to investigate the variables controlling the biomineralization process, and seasonal variation of the hydrochemical factors that affect hydrozincite precipitation. Hydrozincite morphology varies depending on the environmental conditions. Specifically, changes were observed between samples collected in late spring and samples collected in summer, and among samples precipitated under different water flow conditions. According to field observations, correlated with speciation and equilibrium calculation, the maximum intensity in hydrozincite precipitation occurs in the late spring during rainy years. In these conditions, the hydraulic regime in the stream reaches stationary conditions, and saturation index values with respect to hydrozincite reach their highest values. Concomitantly, the Zn 2+ / CO 3 2 - "> CO32- molar ratio reaches values close to 1, indicating that kinetic processes have a role on the hydrozincite biomineralization process.

Sardinia↗

Health effects of energy resources

Energy resources (coal, oil, and natural gas) are among the cornerstones of modern industrial society. The exploitation of these resources, however, is not without costs. Energy materials may contain harmful chemical substances that, if mobilized into air, water, or soil, can adversely impact human health and environmental quality. In order to address the issue of human exposure to toxic substances derived from energy resources, the U.S. Geological Survey (USGS) Energy Resources Program developed a project entitled 'Impacts of Energy Resources on Human Health and Environmental Quality.' The project is intended to provide policymakers and the public with the scientific information needed to weigh the human health and environmental consequences of meeting our energy needs. This fact sheet discusses several areas where the USGS Energy Resources Program is making scientific advances in this endeavor.

Fact Sheet↗

Conceptual modeling framework to support development of site-specific selenium criteria for Lake Koocanusa, Montana, U.S.A., and British Columbia, Canada

The U.S. Geological Survey, working with the Montana Department of Environmental Quality and the British Columbia Ministry of the Environment and Climate Change Strategy, has developed a conceptual modeling framework that can be used to provide structured and scientifically based input to the Lake Koocanusa Monitoring and Research Working Group as they consider potential site-specific selenium criteria for Lake Koocanusa, a transboundary reservoir located in Montana and British Columbia. This report describes that modeling framework, provides an example of how it can be applied, and outlines possible next steps for implementing the framework.

British Columbia, Montana↗

Monarch butterfly (Danaus plexippus) roost site-selection criteria and locations east of the Appalachian Mountains, U.S.A.

The monarch butterfly is a flagship species and pollinator whose populations have declined by 85% in the recent two decades. Their largest population overwinters in Mexico, then disperses across eastern North America during March to August. During September-December, they return south using two flyways, one that spans the central United States and another that follows the Atlantic coast. Migrating monarchs fly diurnally and roost in groups nocturnally. We sought to determine the criteria this species uses to select roost sites, and the landscape context where those sites are found. We developed species distribution models of the landscape context of Atlantic flyway roost sites via citizen scientist observations and environmental variables that affect monarchs in the adult stage prior to migration, using two algorithms (Maximum Entropy and Genetic Algorithm for Ruleset Prediction). We developed two model validation methods: a citizen scientist smartphone application and peer-informed comparisons with aerial imagery. Proximity to surface water, elevation, and vegetative cover were the most important criteria for monarch roost site selection. Our model predicted 2.6 million ha (2.9% of the study area) of suitable roosting habitat in the Atlantic flyway, with the greatest availability along the Atlantic coastal plain and Appalachian Mountain ridges. Conservation of this species is difficult, as monarchs range over both large areas and various habitat types, and most current monarch research and conservation efforts are focused on the breeding and overwintering periods. These models can serve to help prioritize surveys of roosting sites and conservation efforts during the monarchs’ fall migration.

Journal of Insect Behavior↗

Inoculated biocrust cover and functions diverged over a gradient of soil textures and water availability

Restoring biological crust (biocrust) in disturbed drylands is challenging due to the difficult environmental conditions, such as limited soil moisture, low soil nutrients, and extreme temperatures, that impede growth. Understanding how the key components of biocrust—mosses, lichens, and cyanobacteria—react to different environmental factors informs the optimal timing, locations, and species composition for biocrust reintroduction, thereby increasing the likelihood of establishment. Here, we inoculated soils with a diverse range of biocrust organisms, analogous to seeding an area with diverse vascular plant seeds, and varied environmental conditions to observe how these changes influenced the development and functions of reintroduced biocrust. We found that by manipulating soil texture and time spent wet, we can change the proportional cover of biocrust within a restoration-like setting. Specifically, we found that 4 months after inoculation, finer textured soils that received more water become dominated by moss cover, while coarser textured soils with less water remained dominated by cyanobacteria cover, and the interactions between texture and time spent wet strongly influenced cover. We found biocrust morphological group cover had a small, but detectable, effect on ecosystem functions (soil stability and nitrogenase activity, a proxy for nitrogen fixation), but that environmental conditions had a stronger impact on the functions we measured. Manipulative experiments in controlled environments, like this one, can help elucidate the mechanisms underlying the establishment rate and patterns of biocrusts post-inoculation, and inform implementation of inoculations in the field.

New Mexico↗

Development of a multimetric index for assessing the biological condition of the Ohio River

The use of fish communities to assess environmental quality is common for streams, but a standard methodology for large rivers is as yet largely undeveloped. We developed an index to assess the condition of fish assemblages along 1,580 km of the Ohio River. Representative samples of fish assemblages were collected from 709 Ohio River reaches, including 318 "least-impacted" sites, from 1991 to 2001 by means of standardized nighttime boat-electrofishing techniques. We evaluated 55 candidate metrics based on attributes of fish assemblage structure and function to derive a multimetric index of river health. We examined the spatial (by river kilometer) and temporal variability of these metrics and assessed their responsiveness to anthropogenic disturbances, namely, effluents, turbidity, and highly embedded substrates. The resulting Ohio River Fish Index (ORFIn) comprises 13 metrics selected because they responded predictably to measures of human disturbance or reflected desirable features of the Ohio River. We retained two metrics (the number of intolerant species and the number of sucker species [family Catostomidae]) from Karr's original index of biotic integrity. Six metrics were modified from indices developed for the upper Ohio River (the number of native species; number of great-river species; number of centrarchid species; the number of deformities, eroded fins and barbels, lesions, and tumors; percent individuals as simple lithophils; and percent individuals as tolerant species). We also incorporated three trophic metrics (the percent of individuals as detritivores, invertivores, and piscivores), one metric based on catch per unit effort, and one metric based on the percent of individuals as nonindigenous fish species. The ORFIn declined significantly where anthropogenic effects on substrate and water quality were prevalent and was significantly lower in the first 500 m below point source discharges than at least-impacted sites nearby. Although additional research on the temporal stability of the metrics and index will likely enhance the reliability of the ORFIn, its incorporation into Ohio River assessments still represents an improvement over current physicochemical protocols.

Transactions of the American Fisheries Society↗

Estimating national water use associated with unconventional oil and gas development

The U.S. Geological Survey’s (USGS) Water Availability and Use Science Program (WAUSP) goals are to provide a more accurate assessment of the status of the water resources of the United States and assist in the determination of the quantity and quality of water that is available for beneficial uses. These assessments would identify long-term trends or changes in water availability since the 1950s in the United States and help to develop the basis for an improved ability to forecast water avail- ability for future economic, energy-production, and environmental uses. The National Water Census ( http://water.usgs.gov/watercensus/ ), a research program of the WAUSP, supports studies to develop new water accounting tools and assess water availability at the regional and national scales. Studies supported by this program target focus areas with identified water availability concerns and topical science themes related to the use of water within a specific type of environmental setting. The topical study described in this fact sheet will focus on understanding the relation between production of unconventional oil and gas (UOG) for energy and the water needed to produce and sustain this type of energy development. This relation applies to the life-cycle of renewable and nonrenewable forms of UOG energy and includes extraction, production, refinement, delivery, and disposal of waste byproducts. Water-use data and models derived from this topical study will be applied to other similar oil and gas plays within the United States to help resource managers assess and account for water used or needed in these areas. Additionally, the results from this topical study will be used to further refine the methods used in compiling water-use data for selected categories (for example, mining, domestic self-supplied, public supply, and wastewater) in the USGS’s 5-year national water-use estimates reports ( http://water.usgs.gov/watuse/ ).

Fact Sheet↗

Relative influence of flow regime, natural and anthropogenic environment on multidimensional stream fish diversity

The flow regime is considered a ‘master variable’ in riverine ecology because it directly influences stream geomorphology and biological communities. However, other environmental and anthropogenic factors have direct and synergistic effects with flow on fish diversity, complicating estimates of the flow regime's true importance. Moreover, most flow-ecology studies focus only on taxonomic diversity (i.e., species), without considering functional (trait-focused) or phylogenetic (evolution-focused) dimensions of diversity. In this study, we used linear regression with variation partitioning to parse out the independent and shared roles of the flow regime, physical environmental factors (e.g., soil characteristics) and the anthropogenic environment (e.g., developed land cover) for structuring multidimensional diversity of 365 stream fish communities in two biogeographic regions across South Carolina, USA. These variables explained between 8% and 18% of total variation of the local diversity. The flow regime contributed to diversity in all cases, but frequently covaried with physical and/or anthropogenic environmental variables. This covariation indicated that the independent role of flow would have been inflated if other environmental variables were not considered. The three dimensions of stream fish diversity were weakly correlated with one another and were associated with different environmental variables, indicating that each of them represents unique and complimentary facets of fish diversity. Accordingly, only considering the independent effects of instream flow on fish diversity may miss meaningful interactions between the flow regime and the other components of the environment that influence biodiversity patterns, leading to over simplified flow–ecology relationships.

South Carolina↗

Multigenerational, indirect exposure to pyrethroids demonstrates potential compensatory response and reduced toxicity at higher salinity in estuarine fish

Estuarine environments are critical to fish species and serve as nurseries for developing embryos and larvae. They also undergo daily fluctuations in salinity and act as filters for pollutants. Additionally, global climate change (GCC) is altering salinity regimes within estuarine systems through changes in precipitation and sea level rise. GCC is also likely to lead to an increased use of insecticides to prevent pests from damaging agricultural crops as their habitats and mating seasons change from increased temperatures. This underscores the importance of understanding how insecticide toxicity to fish changes under different salinity conditions. In this study, larval Inland Silversides ( Menidia beryllina ) were exposed to bifenthrin (1.1 ng/L), cyfluthrin (0.9 ng/L), or cyhalothrin (0.7 ng/L) at either 6 or 10 practical salinity units (PSU) for 96 h during hatching, with a subset assessed for end points relevant to neurotoxicity and endocrine disruption by testing behavior, gene expression of a select suite of genes, reproduction, and growth. At both salinities, directly exposed F0 larvae were hypoactive relative to the F0 controls; however, the indirectly exposed F1 larvae were hyperactive relative to the F1 control. This could be evidence of a compensatory response to environmentally relevant concentrations of pyrethroids in fish. Effects on development, gene expression, and growth were also observed. Overall, exposure to pyrethroids at 10 PSU resulted in fewer behavioral and endocrine disruptive effects relative to those observed in organisms at 6 PSU.

Environmental Science and Technology↗

Clay mineralogy, fine-grained sediment dispersal, and inferred current patterns, lower Cook Inlet and Kodiak shelf, Alaska

Because lower Cook Inlet and Kodiak shelf are being explored and developed for their petroleum resources, it is essential for environmental reasons to understand the sediment dispersal routes and current patterns. The Susitna River flows into upper Cook Inlet and is the source of clay minerals in Holocene deposits found in western lower Cook Inlet. The Copper River, in the northern Gulf of Alaska, provides clay minerals to the Kodiak shelf and southeastern lower Cook Inlet. In addition, crosion of local bedrock outcrops on the shelf produces some clays that are deposited on the Kodiak shelf. Current patterns can be inferred from the clay-mineral distribution pattern. This is true even if the clay-size fraction is a minor sediment component, and in areas where coarse-grained relict deposits occur. Some potential dangers from offshore petroleum development include: (1) rapid and complete mixing of Cook Inlet waters, (2) adsorption of pollutants by clay deposited in quiet bays, and (3) ion-exchange and adsorption of chemical pollutants on clays that are part of the suspended sediment load in lower Cook Inlet.

Alaska↗

Metal bioavailability models: Current status, lessons learned, considerations for regulatory use, and the path forward

Since the early 2000s, biotic ligand models and related constructs have been a dominant paradigm for risk assessment of aqueous metals in the environment. We critically review 1) the evidence for the mechanistic approach underlying metal bioavailability models; 2) considerations for the use and refinement of bioavailability-based toxicity models; 3) considerations for the incorporation of metal bioavailability models into environmental quality standards; and 4) some consensus recommendations for developing or applying metal bioavailability models. We note that models developed to date have been particularly challenged to accurately incorporate pH effects because they are unique with multiple possible mechanisms. As such, we doubt it is ever appropriate to lump algae/plant and animal bioavailability models; however, it is often reasonable to lump bioavailability models for animals, although aquatic insects may be an exception. Other recommendations include that data generated for model development should consider equilibrium conditions in exposure designs, including food items in combined waterborne–dietary matched chronic exposures. Some potentially important toxicity-modifying factors are currently not represented in bioavailability models and have received insufficient attention in toxicity testing. Temperature is probably of foremost importance; phosphate is likely important in plant and algae models. Acclimation may result in predictions that err on the side of protection. Striking a balance between comprehensive, mechanistically sound models and simplified approaches is a challenge. If empirical bioavailability tools such as multiple-linear regression models and look-up tables are employed in criteria, they should always be informed qualitatively and quantitatively by mechanistic models. If bioavailability models are to be used in environmental regulation, ongoing support and availability for use of the models in the public domain are essential.

Environmental Toxicology and Chemistry↗

The aboveground and belowground growth characteristics of juvenile conifers in the southwestern United States

Juvenile tree survival will play an important role in the persistence of coniferous forests and woodlands in the southwestern United States (SWUS). Vulnerability to climatic and environmental stress declines as trees grow, such that larger, more deeply rooted juveniles are less likely to experience mortality. It is unclear how juvenile conifers partition the aboveground and belowground components of early growth, if growth differs between species and ecosystem types, and what environmental factors influence juvenile carbon allocation above- or belowground. We developed a novel data set for four juvenile conifer groups (junipers, piñon pines, ponderosa pines, firs; 1121 juveniles sampled, 221 destructively) in three height classes (<150 mm, 150–300 mm, and 300+ mm), across 25 SWUS sites. We compared growth characteristics across groups and height classes and related differences to climatic and environmental factors. As tree height increased from <150 mm to 300+ mm, belowground growth increased, root:shoot ratio declined, and specific leaf area declined for all conifers except firs. Maximum rooting depth was shallower than previous estimates (<˜400 mm). Lower elevation juveniles were frequently located in sheltered microsites that provided high shading, whereas mid- and higher elevation juveniles were frequently unsheltered. Across all forest and woodland sites, herbaceous cover was positively correlated with aboveground growth. At study locations comprised of multiple sites, differences in aboveground growth were best explained by ecosystem type (piñon pine-juniper woodland, ponderosa pine forest, mixed-conifer forest) and local environmental variation. Our results indicate generally more belowground early growth and more aboveground later growth, but specific allocation patterns varied among ecosystem (greater proportional shoot growth at lower and mid-elevations compared with higher elevations). Juvenile conifers had similar magnitudes of proportional growth across conifer groups, displaying limited capacity to acclimate growth to differences in climate that control ecosystem type. If juvenile conifers also do not acclimate physiologically to their environment, our findings suggest that local environmental variation will play a primary role in regulating forest and woodland persistence and modify the effects of climate change in the SWUS.

Arizona, Colorado, New Mexico, Utah↗

Forecasting an invasive species’ distribution with global distribution data, local data, and physiological information

Understanding invasive species distributions and potential invasions often requires broad‐scale information on the environmental tolerances of the species. Further, resource managers are often faced with knowing these broad‐scale relationships as well as nuanced environmental factors related to their landscape that influence where an invasive species occurs and potentially could occur. Using invasive buffelgrass ( Cenchrus ciliaris ), we developed global models and local models for Saguaro National Park, Arizona, USA, based on location records and literature on physiological tolerances to environmental factors to investigate whether environmental relationships of a species at a global scale are also important at local scales. In addition to correlative models with five commonly used algorithms, we also developed a model using a priori user‐defined relationships between occurrence and environmental characteristics based on a literature review. All correlative models at both scales performed well based on statistical evaluations. The user‐defined curves closely matched those produced by the correlative models, indicating that the correlative models may be capturing mechanisms driving the distribution of buffelgrass. Given climate projections for the region, both global and local models indicate that conditions at Saguaro National Park may become more suitable for buffelgrass. Combining global and local data with correlative models and physiological information provided a holistic approach to forecasting invasive species distributions.

Arizona↗

Management, morphological, and environmental factors influencing Douglas-fir bark furrows in the Oregon Coast Range

Many land managers in the Pacific Northwest have the goal of increasing late-successional forest structures. Despite the documented importance of Douglas-fir tree bark structure in forested ecosystems, little is known about factors influencing bark development and how foresters can manage development. This study investigated the relative importance of tree size, growth, environmental factors, and thinning on Douglas-fir bark furrow characteristics in the Oregon Coast Range. Bark furrow depth, area, and bark roughness were measured for Douglas-fir trees in young heavily thinned and unthinned sites and compared to older reference sites. We tested models for relationships between bark furrow response and thinning, tree diameter, diameter growth, and environmental factors. Separately, we compared bark responses measured on trees used by bark-foraging birds with trees with no observed usage. Tree diameter and diameter growth were the most important variables in predicting bark characteristics in young trees. Measured environmental variables were not strongly related to bark characteristics. Bark furrow characteristics in old trees were influenced by tree diameter and surrounding tree densities. Young trees used by bark foragers did not have different bark characteristics than unused trees. Efforts to enhance Douglas-fir bark characteristics should emphasize retention of larger diameter trees' growth enhancement.

Oregon↗