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At least 73 records · Page 4Linked to original sources

Tree-ring evidence of forest management moderating drought responses: Implications for dry, coniferous forests in the southwestern United States

Drought, coupled with rising temperatures, is an emerging threat to many forest types across the globe. At least to a degree, we expect management actions that reduce competition (e.g., thinning, prescribed fire, or both) to improve growth of residual trees during drought. The influences of management actions and drought on individual tree growth may be measured with high precision using tree-rings. Here, we summarize tree-ring-based assessments of the effectiveness of thinning and prescribed fire as drought adaptation tools, with special consideration for how these findings might apply to dry coniferous forests in the southwestern United States. The existing literature suggests that thinning treatments generally improve individual tree growth responses to drought, though the literature specific to southwestern coniferous forests is sparse. Assessments from studies beyond the southwestern United States indicate treatment effectiveness varies by thinning intensity, timing of the drought relative to treatments, and individualistic species responses. Several large-scale studies appear to conflict on specifics of how site aridity influences sensitivity to drought following thinning. Prescribed fire effects in the absence of thinning has received much less attention in terms of subsequent drought response. There are limitations for using tree-ring data to estimate drought responses (e.g., difficulties scaling up observations to stand- and landscape-levels). However, tree-rings describe an important dimension of drought effects for individual trees, and when coupled with additional information, such as stable isotopes, aid our understanding of key physiological mechanisms that underlie forest drought response.

Arizona, California, New Mexico, Nevada, Utah↗

Growth strategies and threshold responses to water deficit modulate effects of warming on tree seedlings from forest to alpine

1.Predictions of upslope range shifts for tree species with warming are based on assumptions of moisture stress at lower elevation limits and low temperature stress at high elevation limits. However, recent studies have shown that warming can reduce tree seedling establishment across the entire gradient from subalpine forest to alpine via moisture limitation. Warming effects also vary with species, potentially resulting in community shifts in high elevation forests. 2.We examined the growth and physiology underlying effects of warming on seedling demographic patterns. We evaluated dry mass (DM), root length, allocation above- and belowground, and relative growth rate (RGR) of whole seedlings, and their ability to avoid or endure water stress via water-use efficiency and resisting turgor loss, for Pinus flexilis, Picea engelmannii and Pinus contorta seeded below, at, and above treeline in experimentally warmed, watered, and control plots in the Rocky Mountains, USA. We expected that growth and allocation responses to warming would relate to moisture status and that variation in drought tolerance traits would explain species differences in survival rates. 3.Across treatments and elevations, seedlings of all species had weak turgor-loss resistance, and growth was marginal with negative RGR in the first growth phase (-0.01 to -0.04 g/g/d). Growth was correlated with soil moisture, particularly in the relatively small-seeded P. contorta and P. engelmannii. P. flexilis, known to have the highest survivorship, attained the greatest DM and longest root but was also the slowest growing and most water-use-efficient. This was likely due to its greater reliance on seed reserves. Seedlings developed 15% less total DM, 25% less root DM, and 11% shorter roots in heated compared to unheated plots. Higher temperatures slightly increased DM, root length and RGR where soils were wettest, but more strongly decreased these variables under drier conditions. 4.Synthesis: The surprising heat-inhibition of tree seedling establishment at the cold edge of forests appears to have a physiological basis: newly germinated seedlings have poor moisture stress tolerance, which appears related to marginal initial growth and heavy reliance on seed reserves. Variation in these attributes among tree species at treeline helps explain their different climate responses.

Journal of Ecology↗

Chromosomal damage and EROD induction in tree swallows (Tachycineta bicolor) along the Upper Mississippi River, Minnesota, USA

The health of tree swallows, Tachycineta bicolor, on the Upper Mississippi River (UMR) was assessed in 2010 and 2011 using biomarkers at six sites downriver of Minneapolis/St. Paul, MN metropolitan area, a tributary into the UMR, and a nearby lake. Chromosomal damage was evaluated in nestling blood by measuring the coefficient of variation of DNA content (DNA CV) using flow cytometry. Cytochrome P450 1A activity in nestling liver was measured using the ethoxyresorufin- O -dealkylase (EROD) assay, and oxidative stress was estimated in nestling livers via determination of thiobarbituric acid reacting substances (TBARS), reduced glutathione (GSH), oxidized glutathione (GSSG), the ratio GSSG/GSH, total sulfhydryl, and protein bound sulfhydryl (PBSH). A multilevel regression model (DNA CV) and simple regressions (EROD and oxidative stress) were used to evaluate biomarker responses for each location. Chromosomal damage was significantly elevated at two sites on the UMR (Pigs Eye and Pool 2) relative to the Green Mountain Lake reference site, while the induction of EROD activity was only observed at Pigs Eye. No measures of oxidative stress differed among sites. Multivariate analysis confirmed an increased DNA CV at Pigs Eye and Pool 2, and elevated EROD activity at Pigs Eye. These results suggest that the health of tree swallows has been altered at the DNA level at Pigs Eye and Pool 2 sites, and at the physiological level at Pigs Eye site only.

Minnesota↗

The effects of α-cellulose extraction and blue-stain fungus on retrospective studies of carbon and oxygen isotope variation in live and dead trees†

Tree-ring carbon and oxygen isotope ratios from live and recently dead trees may reveal important mechanisms of tree mortality. However, wood decay in dead trees may alter the δ 13 C and δ 18 O values of whole wood obscuring the isotopic signal associated with factors leading up to and including physiological death. We examined whole sapwood and α-cellulose from live and dead specimens of ponderosa pine ( Pinus ponderosa ), one-seed juniper ( Juniperous monosperma ), piñon pine ( Pinus edulis ) and white fir ( Abies concolor ), including those with fungal growth and beetle frass in the wood, to determine if α-cellulose extraction is necessary for the accurate interpretation of isotopic compositions in the dead trees. We found that the offset between the δ 13 C or δ 18 O values of α-cellulose and whole wood was the same for both live and dead trees across a large range of inter-annual and regional climate differences. The method of α-cellulose extraction, whether Leavitt-Danzer or Standard Brendel modified for small samples, imparts significant differences in the δ 13 C (up to 0.4‰) and δ 18 O (up to 1.2‰) of α-cellulose, as reported by other studies. There was no effect of beetle frass or blue-stain fungus ( Ophiostoma ) on the δ 13 C and δ 18 O of whole wood or α-cellulose. The relationships between whole wood and α-cellulose δ 13 C for ponderosa, piñon and juniper yielded slopes of ~1, while the relationship between δ 18 O of whole wood and α-cellulose was less clear. We conclude that there are few analytical or sampling obstacles to retrospective studies of isotopic patterns of tree mortality in forests of the western United States.

Rapid Communications in Mass Spectrometry↗

Temperate and tropical forest canopies are already functioning beyond their thermal thresholds for photosynthesis

Tropical tree species have evolved under very narrow temperature ranges compared to temperate forest species. Studies suggest that tropical trees may be more vulnerable to continued warming compared to temperate species, as tropical trees have shown declines in growth and photosynthesis at elevated temperatures. However, regional and global vegetation models lack the data needed to accurately represent such physiological responses to increased temperatures, especially for tropical forests. To address this need, we compared instantaneous photosynthetic temperature responses of mature canopy foliage, leaf temperatures, and air temperatures across vertical canopy gradients in three forest types: tropical wet, tropical moist, and temperate deciduous. Temperatures at which maximum photosynthesis occurred were greater in the tropical forests canopies than the temperate canopy (30 ± 0.3 °C vs. 27 ± 0.4 °C). However, contrary to expectations that tropical species would be functioning closer to threshold temperatures, photosynthetic temperature optima was exceeded by maximum daily leaf temperatures, resulting in sub-optimal rates of carbon assimilation for much of the day, especially in upper canopy foliage (>10 m). If trees are unable to thermally acclimate to projected elevated temperatures, these forests may shift from net carbon sinks to sources, with potentially dire implications to climate feedbacks and forest community composition.

Forests↗

Effects and empirical critical loads of Nitrogen for ecoregions of the United States

Human activity in the last century has increased nitrogen (N) deposition to a level that has caused or is likely to cause alterations to the structure and function of many ecosystems across the United States. We synthesized current research relating atmospheric N deposition to effects on terrestrial and freshwater ecosystems in the United States, and estimated associated empirical critical loads of N for several receptors: freshwater diatoms, mycorrhizal fungi, lichens, bryophytes, herbaceous plants, shrubs, and trees. Biogeochemical responses included increased N mineralization and nitrification, increased gaseous N losses, and increased N leaching. Individual species, population, and community responses included increased tissue N, physiological and nutrient imbalances, increased growth, altered root-shoot ratios, increased susceptibility to secondary stresses, altered fire regime, shifts in competitive interactions and community composition, changes in species richness and other measures of biodiversity, and increases in invasive species. The range of critical loads of nutrient N reported for U.S. ecoregions, inland surface waters, and freshwater wetlands is 1–39 kg N ha−1 yr−1, spanning the range of N deposition observed over most of the country. The empirical critical loads of N tend to increase in the following sequence: diatoms, lichens and bryophytes, mycorrhizal fungi, herbaceous plants and shrubs, trees.

Book chapter↗

Drought survival strategies differ between coastal and montane conifers in northern California

Increasingly severe and prolonged droughts are contributing to tree stress and forest mortality across western North America. However, in many cases, we currently have poor information concerning how drought responses in forests vary in relation to competition, climate, and site and tree characteristics. We used annual tree ring evidence of 13 C discrimination (Δ 13 C) and growth metrics to assess drought resistance and resilience for six conifer species at the intersection of several bioregions in northern California. Within each species' range in northern California, we collected competition and tree characteristics from 270 focal trees across sites that varied from wetter to drier habitat conditions (54 sites). Across sites, all six conifer species weathered the severe 2013–2015 drought with reasonably high resistance and post-drought resilience. However, we found important differences in drought responses between coastal and montane species based on annual growth and Δ 13 C metrics. Broadly, the two coastal species showed consistent declines in drought resistance across successive drought years, whereas the four montane species maintained high drought resistance across drought years. More specifically, we found lower Δ 13 C and growth during drought years in coastal species, suggesting stomatal closure during drought with the potential for vulnerability to carbon depletion during long-term drought. Conversely, Δ 13 C and growth were stable in montane species throughout the drought, which may contribute to hydraulic failure under increased drought frequency and/or severity. We also evaluated environmental factors that affect Δ 13 C using data from before and during the drought. These physiological models were consistent for the two coastal species, with a positive relationship between annual precipitation and Δ 13 C and a negative relationship between tree density and Δ 13 C. Conversely, the four montane models illustrated a greater importance of site conditions on drought responses for these species. Our findings show differential risk for drought stress across diverse conifers during severe drought. This work highlights the importance of site and tree characteristics in determining drought responses across cool, annually humid coastal habitats to seasonally dry montane habitats.

California↗

Mechanisms of nitrogen deposition effects on temperate forest lichens and trees

We review the mechanisms of deleterious nitrogen (N) deposition impacts on temperate forests, with a particular focus on trees and lichens. Elevated anthropogenic N deposition to forests has varied effects on individual organisms depending on characteristics both of the N inputs (form, timing, amount) and of the organisms (ecology, physiology) involved. Improved mechanistic knowledge of these effects can aid in developing robust predictions of how organisms respond to either increases or decreases in N deposition. Rising N levels affect forests in micro- and macroscopic ways from physiological responses at the cellular, tissue, and organism levels to influencing individual species and entire communities and ecosystems. A synthesis of these processes forms the basis for the overarching themes of this paper, which focuses on N effects at different levels of biological organization in temperate forests. For lichens, the mechanisms of direct effects of N are relatively well known at cellular, organismal, and community levels, though interactions of N with other stressors merit further research. For trees, effects of N deposition are better understood for N as an acidifying agent than as a nutrient; in both cases, the impacts can reflect direct effects on short time scales and indirect effects mediated through long-term soil and belowground changes. There are many gaps on fundamental N use and cycling in ecosystems, and we highlight the most critical gaps for understanding potential deleterious effects of N deposition. For lichens, these gaps include both how N affects specific metabolic pathways and how N is metabolized. For trees, these gaps include understanding the direct effects of N deposition onto forest canopies, the sensitivity of different tree species and mycorrhizal symbionts to N, the influence of soil properties, and the reversibility of N and acidification effects on plants and soils. Continued study of how these N response mechanisms interact with one another, and with other dimensions of global change, remains essential for predicting ongoing changes in lichen and tree populations across North American temperate forests.

Ecosphere↗

Biophysical mechanisms of trichloroethene uptake and loss in baldcypress growing in shallow contaminated groundwater

Wetland vegetation may be useful in the remediation of shallow contaminated aquifers. Mesocosm experiments were conducted to describe the regulatory mechanisms affecting trichloroethene (TCE) removal rates from groundwater by flood-adapted wetland trees at a contaminated site. TCE flux through baldcypress [Taxodium distichum (L) Rich] seedlings grown in glass- carboys decreased from day to night and from August to December. The diel fluctuation coincided with changes in leaf-level physiology, as the daytime flux was significantly correlated with net photosynthesis but not with respiration at night. A decrease in seedling water use from summer to winter explained the large seasonal difference in TCE flux. A simple model that simulates gas-phase diffusion through aerenchyma tested the importance of diffusion of TCE vapor from roots to the stem. The modeled diffusive flux was within 64% of the observed value during the winter but could only explain 8% of the summer flux. Seedling water use was a good estimator of flux during the summer. Hence, evapotranspiration (ET) in the summer may serve as a good predictor for the potential of TCE removal by baldcypress trees, while diffusive flux may better approximate potential contaminant loss in the winter.Wetland vegetation may be useful in the remediation of shallow contaminated aquifers. Mesocosm experiments were conducted to describe the regulatory mechanisms affecting trichloroethene (TCE) removal rates from groundwater by flood-adapted wetland trees at a contaminated site. TCE flux through baldcypress [Taxodium distichum (L) Rich] seedlings grown in glass-carboys decreased from day to night and from August to December. The diel fluctuation coincided with changes in leaf-level physiology, as the daytime flux was significantly correlated with net photosynthesis but not with respiration at night. A decrease in seedling water use from summer to winter explained the large seasonal difference in TCE flux. A simple model that simulates gas-phase diffusion through aerenchyma tested the importance of diffusion of TCE vapor from roots to the stem. The modeled diffusive flux was within 64% of the observed value during the winter but could only explain 8% of the summer flux. Seedling water use was a good estimator of flux during the summer. Hence, evapotranspiration (ET) in the summer may serve as a good predictor for the potential of TCE removal by baldcypress trees, while diffusive flux may better approximate potential contaminant loss in the winter.

Environmental Science & Technology↗

Multi-scale predictions of massive conifer mortality due to chronic temperature rise

Global temperature rise and extremes accompanying drought threaten forests and their associated climatic feedbacks. Our ability to accurately simulate drought-induced forest impacts remains highly uncertain in part owing to our failure to integrate physiological measurements, regional-scale models, and dynamic global vegetation models (DGVMs). Here we show consistent predictions of widespread mortality of needleleaf evergreen trees (NET) within Southwest USA by 2100 using state-of-the-art models evaluated against empirical data sets. Experimentally, dominant Southwest USA NET species died when they fell below predawn water potential ( Ψ pd ) thresholds (April–August mean) beyond which photosynthesis, hydraulic and stomatal conductance, and carbohydrate availability approached zero. The evaluated regional models accurately predicted NET Ψ pd , and 91% of predictions (10 out of 11) exceeded mortality thresholds within the twenty-first century due to temperature rise. The independent DGVMs predicted ≥50% loss of Northern Hemisphere NET by 2100, consistent with the NET findings for Southwest USA. Notably, the global models underestimated future mortality within Southwest USA, highlighting that predictions of future mortality within global models may be underestimates. Taken together, the validated regional predictions and the global simulations predict widespread conifer loss in coming decades under projected global warming.

Nature Climate Change↗

Editorial: Contributions of behavior and physiology to conservation biology

Conservation biology is a rapidly evolving discipline, with its synthetic, multidisciplinary framework expanding extensively in recent years. Seemingly disparate disciplines, such as behavior and physiology, are being integrated into this discipline's growing portfolio, resulting in diverse tools that can help develop conservation solutions. Behavior and physiology have traditionally been considered separate fields, yet their integration can provide a more comprehensive approach to developing solutions to conservation and management problems. However, demonstrations are needed of how behavior and physiology—either separately or combined—have contributed to conservation success. Examining species' vulnerabilities to extinction through the lenses of behavior and physiology can provide insight into the mechanisms that drive population declines and extirpations. Our goal is to increase awareness of the benefit of combining behavioral and physiological tools to improve conservation management decisions. Such studies can also help strengthen the basis for evidence-based conservation which, in some cases, has been previously lacking. The diverse studies in our Research Topic illustrate key examples of ways that behavior and physiology can be incorporated into conservation biology. Three main themes emerged from the invited papers with respect to their relevance to conservation: (1) Stress physiology, (2) indicators of health and disease dynamics, (3) and movement ecology. But these themes were also intertwined, thus showing the importance of integrating multiple fields of research to successfully address questions about conservation biology. Two mini reviews discuss the importance of examining how stress physiology may affect individual fitness and capacity to cope with change which, ultimately, affects the resiliency of populations. Walls and Gabor, in their mini review, promoted combining studies of behavior and physiology to aid in developing conservation strategies for amphibians, which could provide conservation managers with workable solutions to global environmental change. Walls and Gabor also pointed out that studies of behavior are useful to understand how native amphibian species respond to invasive predators. This is supported by Roznik et al. who combined physiology and behavior for a more holistic understanding of the impacts of competition between native and invasive frogs. These authors found that invasive Cuban tree frogs outperformed two native species in jump length and speed, offsetting the costs of dehydration. This work also ties in well to a review by Joly, who indicated the importance of exploring movement ecology (on multiple spatial scales) of invasive species to provide insights for invasive species management. Walls and Gabor also emphasized that more studies on phenological shifts of species in response to recent climate change are needed, as explored by Bókony et al. with birds. These authors found that bird species varied their migratory behaviors in response to contemporary climate change which, in turn, influenced subsequent trends in population size.

Frontiers in Ecology and Evolution↗

The soil creep-curved tree fallacy

No evidence has been found in eastern deciduous forests to substantiate statements that curved tree trunks, downslope tilted tree trunks, and upslope trailing tree root systems are the result of soil creep. Curvature and tilting of trunks are described as geotropic and phototropic responses to physical and physiological conditions unrelated to soil creep. The occurrence of upslope trailing of roots has not been demonstrated.

Journal of Research of the U.S. Geological Survey↗

Exposure and effects of bioaccumulative contaminants of emerging concern in tree swallows nesting across the Laurentian Great Lakes

Contaminants of emerging concern (CECs) are a loosely defined group of chemicals whose wide-spread usage or presence in the environment has occurred more recently or for which there has been relatively little research done until recently. Many of these CECs are not currently regulated. The National Toxicology Program within the U.S. Department of Health and Human Services estimates that about 2000 CECs are introduced each year (https://ntp.niehs.nih.gov/about/). An unknown number may pose a risk to human or animal health. The Phase 1 (2010 – 2014) CEC work in birds, which is the subject of this report, assessed exposure across the Great Lakes to polybrominated diphenyl ethers (PBDEs), perfluorinated compounds (PFASs), and polycyclic aromatic hydrocarbons (PAHs), and put those exposures into context with data from biologically relevant endpoints such as reproductive success, as well as, physiological response indicators (bioindicators) to assess possible effects. The group of chemicals included in Phase 1 were mainly those chemicals that bioaccumulate in tissues. Phase 2 (2015 – 2019) CEC work with tree swallows was expanded to include CECs whose occurrence in the environment is more temporary or seasonal, and that do not necessarily bioaccumulate. These are often called pseudo-persistent, because, while they are not long-lived in the environment, there are often daily inputs via waste water treatment plants, and run-off from farm fields and storm drainages, thereby making them available to biota year-round. These include pharmaceuticals, personal care products, and newer pesticides including herbicides. Tree swallow work on these less persistent CECs will be reported in the future, however see other Appendices in this report for information on some of these types of CECs (Appendices A, B, D).

Laurentian Great Lakes↗

A dirty dozen ways to die: Metrics and modifiers of mortality driven by drought and warming for a tree species

Tree mortality events driven by drought and warmer temperature, often amplified by pests and pathogens, are emerging as one of the predominant climate change impacts on plants. Understanding and predicting widespread tree mortality events in the future is vital as they affect ecosystem goods and services provided by forests and woodlands, including carbon storage needed to help offset warming. Additionally, if extensive enough, tree die-off events can influence not only local climate but also climate and vegetation elsewhere via ecoclimate teleconnections. Consequently, recent efforts have focused on improving predictions of tree mortality. One of the most commercially important genera of trees is Pinus , and the most studied species globally for drought-induced tree mortality is piñon pine, Pinus edulis . Numerous metrics have been developed in association with predicting mortality thresholds or variations in mortality for this species. In this article, we compiled metrics associated with drought and warming related mortality that were developed for P. edulis or for which P. edulis was a key example species used in a calculation or prediction. We grouped these metrics into three categories: (i) those related to simple climate variables, (ii) those related to physiological responses, and (iii) those that require multi-step calculations or modeling using climate, ecohydrological, and/or ecophysiological data; and we identified the spatial-temporal scale of each of these metrics. We also compiled factors shown to modify rates or sensitivities of mortality. The metrics to predict mortality include empirical ones which often have implicit linkages to expected mechanisms, and more mechanistic ones related to physiological drivers. The metrics for P. edulis have similarities with those available for other species of Pinus . Expected future mortality events will provide an opportunity to observationally and experimentally test and compare these metrics related to tree mortality for P. edulis via near-term ecological forecasting. The metrics for P. edulis may also be useful as potential analogs for other genera. Improving predictions of tree mortality for this species and others will be increasingly important as an aid to move toward anticipatory management.

Frontiers in Forests and Global Change↗

Darcy’s law predicts widespread forest mortality under climate warming

Drought and heat-induced tree mortality is accelerating in many forest biomes as a consequence of a warming climate, resulting in a threat to global forests unlike any in recorded history . Forests store the majority of terrestrial carbon, thus their loss may have significant and sustained impacts on the global carbon cycle . We use a hydraulic corollary to Darcy ’ s law, a core principle of vascular plant physiology , to predict characteristics of plants that will survive and die during drought under warmer future climates. Plants that are tall with isohydric stomatal regulation, low hydraulic conductance, and high leaf area are most likely to die from future drought stress. Thus, tall trees of old-growth forests are at the greatest risk of loss, which has ominous implications for terrestrial carbon storage. This application of Darcy ’ s law indicates today ’ s forests generally should be replaced by shorter and more xeric plants, owing to future warmer droughts and associated wildfires and pest attacks. The Darcy ’ s corollary also provides a simple, robust framework for informing forest management interventions needed to promote the survival of current forests. Given the robustness of Darcy ’ s law for predictions of vascular plant function, we conclude with high certainty that today ’ s forests are going to be subject to continued increases in mortality rates that will result in substantial reorganization of their structure and carbon storage.

Nature Climate Change↗

The effect of nutrient enrichment on growth, photosynthesis and hydraulic conductance of dwarf mangroves in Panamá

1. Dwarf stands of the mangrove Rhizophora mangle L. are extensive in the Caribbean. We fertilized dwarf trees in Almirante Bay, Bocas del Toro Province, north-eastern Panama with nitrogen (N) and phosphorus (P) to determine (1) if growth limitations are due to nutrient deficiency; and (2) what morphological and/or physiological factors underlie nutrient limitations to growth. 2. Shoot growth was 10-fold when fertilized with P and twofold with N fertilization, indicating that stunted growth of these mangroves is partially due to nutrient deficiency. 3. Growth enhancements caused by N or P enrichment could not be attributed to increases in photosynthesis on a leaf area basis, although photosynthetic nutrient-use efficiency was improved. The most dramatic effect was on stem hydraulic conductance, which was increased sixfold by P and 2-5-fold with N enrichment. Fertilization with P enhanced leaf and stem P concentrations and reduced C:N ratio, but did not alter leaf damage by herbivores. 4. Our findings indicate that addition of N and P significantly alter tree growth and internal nutrient dynamics of mangroves at Bocas del Toro, but also that the magnitude, pattern and mechanisms of change will be differentially affected by each nutrient.

Functional Ecology↗

Seasonal and species-level water-use strategies and groundwater dependence in dryland riparian woodlands during extreme drought

Drought-induced groundwater decline and warming associated with climate change are primary threats to dryland riparian woodlands. We used the extreme 2012–2019 drought in southern California as a natural experiment to assess how differences in water-use strategies and groundwater dependence may influence the drought susceptibility of dryland riparian tree species with overlapping distributions. We analyzed tree-ring stable carbon and oxygen isotopes collected from two cottonwood species ( Populus trichocarpa and P . fremontii ) along the semi-arid Santa Clara River. We also modeled tree source water δ 18 O composition to compare with observed source water δ 18 O within the floodplain to infer patterns of groundwater reliance. Our results suggest that both species functioned as facultative phreatophytes that used shallow soil moisture when available but ultimately relied on groundwater to maintain physiological function during drought. We also observed apparent species differences in water-use strategies and groundwater dependence related to their regional distributions. P . fremontii was constrained to more arid river segments and ostensibly used a greater proportion of groundwater to satisfy higher evaporative demand. P . fremontii maintained ∆ 13 C at pre-drought levels up until the peak of the drought, when trees experienced a precipitous decline in ∆ 13 C. This response pattern suggests that trees prioritized maintaining photosynthetic processes over hydraulic safety, until a critical point. In contrast, P . trichocarpa showed a more gradual and sustained reduction in ∆ 13 C, indicating that drought conditions induced stomatal closure and higher water use efficiency. This strategy may confer drought avoidance for P . trichocarpa while increasing its susceptibility to anticipated climate warming.

California↗

Only sun-lit leaves of the uppermost canopy exceed both air temperature and photosynthetic thermal optima in a wet tropical forest

Tropical forests have evolved under relatively narrow temperature regimes, and therefore may be more susceptible to climatic change than forests in higher latitudes. Recent evidence shows that lowland tropical forest canopies may already be exceeding thermal maxima for photosynthesis. Height can strongly influence both the microclimate and physiology of forest canopy foliage, yet vertical trends in canopy micrometeorology are rarely examined in tropical forests. To improve our understanding of how climatological and micrometeorological conditions affect tropical tree function, we assessed vertical gradients of photosynthetic photon flux density, vapor pressure deficit, air temperature, leaf temperature, and the difference between leaf and air temperature (ΔT) in a Puerto Rican tropical wet forest. Both air temperature and vapor pressure deficit increased linearly with height. Leaf temperature, however, did not significantly differ across the shaded foliage from 0-16 m, while the uppermost layer (20 m) was up to 4°C hotter than the rest of the foliage and up to 5°C hotter than air temperature at the highest radiation intensity. As a result, leaf temperatures in the shaded middle canopy and understory showed nearly poikilothermic behavior (i.e., leaf temperatures = air temperature), while the uppermost canopy strata showed megathermic behavior (i.e., leaf temperatures greater than air temperature), revealing different thermoregulation strategies for sun-lit versus shaded foliage. In addition, the uppermost canopy was the only stratum to exceed mean photosynthetic temperature optima for this site (T opt = 30.2 ± 1.1°C). Because the upper canopy plays a disproportionately large role in whole-forest photosynthesis, continued warming could potentially weaken the tropics’ carbon sink capacity. However, the shaded leaves may be able increase carbon uptake with further warming because they appear to be able to maintain temperatures below photosynthetic optima, possibly with the help of radiation shielding provided by the uppermost canopy layer.

Agricultural and Forest Meteorology↗