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At least 73 records · Page 4Linked to original sources

Long-distance transport of Hg, Sb, and As from a mined area, conversion of Hg to methyl-Hg, and uptake of Hg by fish on the Tiber River basin, west-central Italy

Stream sediment, stream water, and fish were collected from a broad region to evaluate downstream transport and dispersion of mercury (Hg) from inactive mines in the Monte Amiata Hg District (MAMD), Tuscany, Italy. Stream sediment samples ranged in Hg concentration from 20 to 1,900 ng/g, and only 5 of the 17 collected samples exceeded the probable effect concentration for Hg of 1,060 ng/g, above which harmful effects are likely to be observed in sediment-dwelling organisms. Concentrations of methyl-Hg in Tiber River sediment varied from 0.12 to 0.52 ng/g, and although there is no established guideline for sediment methyl-Hg, these concentrations exceeded methyl-Hg in a regional baseline site (<0.02 ng/g). Concentrations of Hg in stream water varied from 1.2 to 320 ng/L, all of which were below the 1,000 ng/L Italian drinking water Hg guideline and the 770 ng/L U.S. Environmental Protection Agency (USEPA) guideline recommended to protect against chronic effects to aquatic wildlife. Methyl-Hg concentrations in stream water varied from <0.02 to 0.53 ng/L and were generally elevated compared to the baseline site (<0.02 ng/L). All stream water samples contained concentrations of As (<1.0–6.2 μg/L) and Sb (<0.20–0.37 μg/L) below international drinking water guidelines to protect human health (10 μg/L for As and 20 μg/L for Sb) and for protection against chronic effects to aquatic wildlife (150 μg/L for As and 5.6 μg/L for Sb). Concentrations of Hg in freshwater fish muscle ranged from 0.052–0.56 μg/g (wet weight), mean of 0.17 μg/g, but only 17 % (9 of 54) exceeded the 0.30 μg/g (wet weight) USEPA fish muscle guideline recommended to protect human health. Concentrations of Hg in freshwater fish in this region generally decreased with increasing distance from the MAMD, where fish with the highest Hg concentrations were collected more proximal to the MAMD, whereas all fish collected most distal from Hg mines contained Hg below the 0.30 μg/g fish muscle guideline. Data in this study indicate some conversion of inorganic Hg to methyl-Hg and uptake of Hg in fish on the Paglia River, but less methylation of Hg and Hg uptake by freshwater fish in the larger Tiber River.

Tiber River Basin↗

A chemo-mechanical snapshot of in-situ conversion of kerogen to petroleum

Organic matter (OM) from various biogenic origins converts to solid bitumen in-situ when it undergoes thermal maturation. It is well documented that during this process, the ratios of both hydrogen and oxygen to carbon will decrease, resulting in an increase in OM aromaticity and molecular chemo-mechanical homogeneity. Although there have been extensive efforts to reveal molecular alteration occurring to OM during conversion, in-situ and continuous observation of such alterations on naturally occurring samples is missing. Therefore, evaluation of previous results cannot be made independent from natural sample variability. In this study, we identified OM particles ( Tasmanites ) that are evolving in-situ into solid bitumen in the Bakken Formation. This in-situ bituminization allows examination of a continuous transformation in OM molecular structure at micron-scale using AFM based IR spectroscopy applied at the transition/interface zone. Moreover, contact mode in the AFM was employed to reveal and relate changes in mechanical properties at a similar scale of measurement. Understanding these chemical and mechanical alterations is important to understand shale reservoir properties and better explain hydrocarbon generation, expulsion, and migration processes at the microscale.

Geochimica et Cosmochimica Acta↗

Multisample conversion of water to hydrogen by zinc for stable isotope determination

Two techniques for the conversion of water to hydrogen for stable isotope ratio determination have been developed that are especially suited for automated multisample analysis. Both procedures involve reaction of zinc shot with a water sample at 450 ??C. in one method designed for water samples in bottles, the water is put in capillaries and is reduced by zinc in reaction vessels; overall savings in sample preparation labor of 75% have been realized over the standard uranium reduction technique. The second technique is for waters evolved under vacuum and is a sealed-tube method employing 9 mm o.d. quartz tubing. Problems inherent with zinc reduction include surface inhomogeneity of the zinc and exchange of hydrogen both with the zinc and with the glass walls of the vessels. For best results, water/zinc and water/glass surface area ratios of vessels should be kept as large as possible.

Analytical Chemistry↗

A simplified algorithm for temperature conversions

The author is surprised that those who teach temperature conversion formulas have not introduced the easier-to-retain relationship based on the fact that –40 F° is the same temperature as –40 C°.

Journal of Chemical Education↗

Direct conversion of terpenylalkanolamines to ethylidyne N -nitroso compounds

A series of mono- and diterpenylalkanolamines bearing isopropylidene functionality on the terpene group was reacted with sodium nitrite in aqueous acetic acid to yield ethylidyne N -nitroso analogues. The key feature of this direct conversion involved initial N-nitrosation followed by apparent elimination of a "CH 4 " unit (not necessarily methane) from the isopropylidene double bond. The product distribution data for ethylidyne nitrosamines derived from tertiary terpenyl alkanolamines reflect the conformational outcome of the nitrosative dealkylation process. For &beta; , &gamma; -unsaturated allylic diterpenylethanolamines, electronic effects appeared to be important for controlling the product distribution of ethylidyne nitrosamines in light of the highly selective &alpha; -cleavage observed in the nitrosation reactions.

Journal of Organic Chemistry↗

Activity-based, genome-resolved metagenomics uncovers key populations and pathways involved in subsurface conversions of coal to methane

Microbial metabolisms and interactions that facilitate subsurface conversions of recalcitrant carbon to methane are poorly understood. We deployed an in situ enrichment device in a subsurface coal seam in the Powder River Basin (PRB), USA, and used BONCAT-FACS-Metagenomics to identify translationally active populations involved in methane generation from a variety of coal-derived aromatic hydrocarbons. From the active fraction, high-quality metagenome-assembled genomes (MAGs) were recovered for the acetoclastic methanogen, Methanothrix paradoxum , and a novel member of the Chlorobi with the potential to generate acetate via the Pta-Ack pathway. Members of the Bacteroides and Geobacter also encoded Pta-Ack and together, all four populations had the putative ability to degrade ethylbenzene, phenylphosphate, phenylethanol, toluene, xylene, and phenol. Metabolic reconstructions, gene analyses, and environmental parameters also indicated that redox fluctuations likely promote facultative energy metabolisms in the coal seam. The active “ Chlorobi PRB” MAG encoded enzymes for fermentation, nitrate reduction, and multiple oxygenases with varying binding affinities for oxygen. “ M. paradoxum PRB” encoded an extradiol dioxygenase for aerobic phenylacetate degradation, which was also present in previously published Methanothrix genomes. These observations outline underlying processes for bio-methane from subbituminous coal by translationally active populations and demonstrate activity-based metagenomics as a powerful strategy in next generation physiology to understand ecologically relevant microbial populations.

Montana, Wyoming↗

Antemortem detection of chronic wasting disease prions in nasal brush collections and rectal biopsies from white-tailed deer by real time quaking-induced conversion

Chronic wasting disease (CWD), a transmissible spongiform encephalopathy of cervids, was first documented nearly 50 years ago in Colorado and Wyoming and has since spread to cervids in 23 states, two Canadian provinces, and the Republic of Korea. The expansion of this disease makes the development of sensitive diagnostic assays and antemortem sampling techniques crucial for the mitigation of its spread; this is especially true in cases of relocation/reintroduction of farmed or free-ranging deer and elk or surveillance studies of private or protected herds, where depopulation is contraindicated. This study sought to evaluate the sensitivity of the real-time quaking-induced conversion (RT-QuIC) assay by using recto-anal mucosa-associated lymphoid tissue (RAMALT) biopsy specimens and nasal brush samples collected antemortem from farmed white-tailed deer ( n = 409). Antemortem findings were then compared to results from ante- and postmortem samples (RAMALT, brainstem, and medial retropharyngeal lymph nodes) evaluated by using the current gold standard in vitro assay, immunohistochemistry (IHC) analysis. We hypothesized that the sensitivity of RT-QuIC would be comparable to IHC analysis in antemortem tissues and would correlate with both the genotype and the stage of clinical disease. Our results showed that RAMALT testing by RT-QuIC assay had the highest sensitivity (69.8%) compared to that of postmortem testing, with a specificity of >93.9%. These data suggest that RT-QuIC, like IHC analysis, is an effective assay for detection of PrP CWD in rectal biopsy specimens and other antemortem samples and, with further research to identify more sensitive tissues, bodily fluids, or experimental conditions, has potential for large-scale and rapid automated testing for CWD diagnosis.

Journal of Clinical Microbiology↗

Seeded amplification of chronic wasting disease prions in nasal brushings and recto-anal mucosal associated lymphoid tissues from elk by real time quaking-induced conversion

Chronic wasting disease (CWD), a transmissible spongiform encephalopathy of cervids, was first documented nearly 50 years ago in Colorado and Wyoming and has since been detected across North America and the Republic of Korea. The expansion of this disease makes the development of sensitive diagnostic assays and antemortem sampling techniques crucial for the mitigation of its spread; this is especially true in cases of relocation/reintroduction or prevalence studies of large or protected herds, where depopulation may be contraindicated. This study evaluated the sensitivity of the real-time quaking-induced conversion (RT-QuIC) assay of recto-anal mucosa-associated lymphoid tissue (RAMALT) biopsy specimens and nasal brushings collected antemortem. These findings were compared to results of immunohistochemistry (IHC) analysis of ante- and postmortem samples. RAMALT samples were collected from populations of farmed and free-ranging Rocky Mountain elk ( Cervus elaphus nelsoni ; n = 323), and nasal brush samples were collected from a subpopulation of these animals ( n = 205). We hypothesized that the sensitivity of RT-QuIC would be comparable to that of IHC analysis of RAMALT and would correspond to that of IHC analysis of postmortem tissues. We found RAMALT sensitivity (77.3%) to be highly correlative between RT-QuIC and IHC analysis. Sensitivity was lower when testing nasal brushings (34%), though both RAMALT and nasal brush test sensitivities were dependent on both the PRNP genotype and disease progression determined by the obex score. These data suggest that RT-QuIC, like IHC analysis, is a relatively sensitive assay for detection of CWD prions in RAMALT biopsy specimens and, with further investigation, has potential for large-scale and rapid automated testing of antemortem samples for CWD.

Journal of Clinical Microbiology↗

Supplemental materials for the ICDP-USGS Eyreville A, B, and C core holes, Chesapeake Bay impact structure: Core-box photographs, coring-run tables, and depth-conversion files

During 2005-2006, the International Continental Scientific Drilling Program and the U.S. Geological Survey drilled three continuous core holes into the Chesapeake Bay impact structure to a total depth of 1766.3 m. A collection of supplemental materials that presents a record of the core recovery and measurement data for the Eyreville cores is available on CD-ROM at the end of this volume and in the GSA Data Repository. The supplemental materials on the CD-ROM include digital photographs of each core box from the three core holes, tables of the three coring-run logs, as recorded on site, and a set of depth-conversion programs. In this chapter, the contents, purposes, and basic applications of the supplemental materials are briefly described. With this information, users can quickly decide if the materials will apply to their specific research needs.

Chesapeake Bay↗

Vegetation type conversion in the US Southwest: Frontline observations and management responses

Forest and nonforest ecosystems of the western United States are experiencing major transformations in response to land-use change, climate warming, and their interactive effects with wildland fire. Some ecosystems are transitioning to persistent alternative types, hereafter called “vegetation type conversion” (VTC). VTC is one of the most pressing management issues in the southwestern US, yet current strategies to intervene and address change often use trial-and-error approaches devised after the fact. To better understand how to manage VTC, we gathered managers, scientists, and practitioners from across the southwestern US to collect their experiences with VTC challenges, management responses, and outcomes.

Arizona, California, Colorado, New Mexico↗

Land conversion and pesticide use degrade forage areas for honey bees in America’s beekeeping epicenter

A diverse range of threats have been associated with managed bee declines globally. Recent increases of two known threats, land-use change and pesticide use, have resulted from agricultural expansion and intensification notably in the top honey producing state in the United States (U.S.): North Dakota. This study investigated the dual threat from land conversion and pesticide use surrounding ~14,000 registered apiaries in North Dakota from 2001 to 2014. We estimated the annual total insecticide use (kg) on major crops within 1.6 km of apiary sites. Of the eight insecticides, six showed significant increasing trends over the time period. Specifically, applications of the newly established neonicotinoids Chlothianidin, Imidacloprid and Thiamethoxam, increased annually by 1329 kg, 686 kg, 795 kg, respectively. Also, the use of Chlorpyrifos, which was well-established by 2001 and is highly toxic to honey bees, increased by ~8,800 kg annually from 6,500 kg in 2001 to 115,000 kg in 2014 on corn, soybeans and wheat. We further evaluated the relative quality changes of natural/semi-natural land covers surrounding apiaries in 2006, 2010 and 2014, a period of significant increases in cropland area. In areas surrounding apiaries, we observed changes in multiple indices of forage quality that reflect the deteriorating landscape surrounding registered apiary sites due to land-use change and pesticide-use increases. Overall, our results suggest that the application of foliar-applied insecticides, including pyrethroids and one organophosphate, increased surrounding apiaries when the use of neonicotinoid seed treatment surged and the area for producing corn and soybeans expanded. Spatially, these threats were most pronounced in southeastern North Dakota, a region hosting a high density of apiary sites that has recently experienced corn and soybean expansion. Our results highlight the values of natural and semi-natural land covers as sources of pollinator forage and providing refugia for bees against pesticide exposure. Our study provides insights for targeting conservation efforts to improve forage quality for benefiting managed pollinators.

North Dakota↗

Validation of a real-time quaking-induced conversion (RT-QuIC) assay protocol to detect chronic wasting disease using rectal mucosa of naturally infected, pre-clinical white-tailed deer (Odocoileus virginianus)

Chronic wasting disease (CWD) is a fatal prion disease of cervids spreading across North America. More effective mitigation efforts may require expansion of the available toolkit to include new methods that provide earlier antemortem detection, higher throughput, and less expense than current immunohistochemistry (IHC) methods. The rectal mucosa near the rectoanal junction is a site of early accumulation of CWD prions and is safely sampled in living animals by pinch biopsy. A fluorescence-based, 96-well format, protein-aggregation assay-the real-time quaking-induced conversion (RT-QuIC) assay-is capable of ultra-sensitive detection of CWD prions. Notably, the recombinant protein substrate is crucial to the assay's performance and is now commercially available. In this blinded independent study, the preclinical diagnostic performance of a standardized RT-QuIC protocol using a commercially sourced substrate (MNPROtein) and a laboratory-produced substrate was studied using mock biopsy samples of the rectal mucosa from 284 white-tailed deer ( Odocoileus virginianus ). The samples were from a frozen archive of intact rectoanal junctions collected at depopulations of farmed herds positive for CWD in the United States. All deer were pre-clinical at the time of depopulation and infection status was established from the regulatory record, which evaluated the medial retropharyngeal lymph nodes (MRPLNs) and obex by CWD-IHC. A pre-analytic sample precipitation step was found to enhance the protocol's detection limit. Performance metrics were influenced by the choice of RT-QuIC diagnostic cut points (minimum number of positive wells and assay time) and by deer attributes (preclinical infection stage and prion protein genotype). The peak overall diagnostic sensitivities of the protocol were similar for both substrates (MNPROtein, 76.8%; laboratory-produced, 73.2%), though each was achieved at different cut points. Preclinical infection stage and prion protein genotype at codon 96 (G = glycine, S = serine) were primary predictors of sensitivity. The diagnostic sensitivities in late preclinical infections (CWD-IHC positive MPRLNs and obex) were similar, ranging from 96% in GG96 deer to 80% in xS96 deer (x = G or S). In early preclinical infections (CWD-IHC positive MRPLNs only), the diagnostic sensitivity was 64-71% in GG96 deer but only 25% in xS96 deer. These results demonstrate that this standardized RT-QuIC protocol for rectal biopsy samples using a commercial source of substrate produced stratified diagnostic sensitivities similar to or greater than those reported for CWD-IHC but in less than 30 hours of assay time and in a 96-well format. Notably, the RT-QuIC protocol used herein represents a standardization of protocols from several previous studies. Alignment of the sensitivities across these studies suggests the diagnostic performance of the assay is robust given quality reagents, optimized diagnostic criteria, and experienced staff.

PLoS ONE↗

Analog-to-digital conversion as a source of drifts in displacements derived from digital recordings of ground acceleration

Displacements obtained from double integration of digitally recorded ground accelerations often show drifts much larger than those expected for the true ground displacements. These drifts might be due to many things, including dynamic elastic ground tilt, inelastic ground deformation, hysteresis in the instruments, and cross feed due to misalignment of nominally orthogonal sensors. This article shows that even if those effects were not present, the analog-to-digital conversion (ADC) process can produce apparent "pulses" and offsets in the acceleration baseline if the ground motion is slowly varying compared with the quantization level of the digitization. Such slowly varying signals can be produced by constant offsets that do not coincide with a quantization level and by near- and intermediate-field terms in the wave field radiated from earthquakes. Double integration of these apparent pulses and offsets leads to drifts in the displacements similar to those found in processing real recordings. These effects decrease in importance as the resolution of the ADC process increases.

Bulletin of the Seismological Society of America↗

A big problem for small earthquakes: Benchmarking routine magnitudes and conversion relationships with coda-envelope-derived Mw in southern Kansas and northern Oklahoma

Earthquake magnitudes are widely relied upon measures of earthquake size. Although moment magnitude ( ⁠ M w "> M w Mw ⁠ ) has become the established standard for moderate and large earthquakes, difficulty in reliably measuring seismic moments for small (generally M w &lt; 4 "> M w < 4 Mw<4 ⁠ ) earthquakes has meant that magnitudes for these events remain plagued by a patchwork of inconsistent measurement scales. Because of this, magnitudes of small earthquakes and statistics derived from them can be biased. Furthermore, because small earthquakes are much more numerous than large ones, many applications, such as seismic hazard modeling, depend critically on analysis of events characterized by magnitudes other than M w "> M w Mw ⁠ . To assess this problem, we apply coda envelope analysis to reliably determine moment magnitudes for a case study of small earthquakes from northern Oklahoma and southern Kansas. Not surprisingly, we find significant differences among M L "> M L ML ⁠ , m b L g "> m b L g mbLg ⁠ , and M w "> M w Mw for M ∼2–4 earthquakes examined here. More troublingly, we find that relations designed to convert other magnitudes to M w "> M w Mw ⁠ , which are relied upon for important applications such as seismic hazard analysis, often increase rather than decrease this bias for our dataset. In our case study, we find that converted magnitudes can result in a systematic bias sometimes exceeding 0.5 magnitude units, a difference that typically corresponds to a factor of ∼3 in seismicity rate. Moreover, we find a correspondingly large bias in Gutenberg–Richter b ‐values, controlled primarily by inaccurate magnitude scaling in the conversion relationships. Although this study focuses on a relatively small geographic area, we can expect that similar issues exist with varying severity in other regions. Therefore, magnitudes of small earthquakes and their associated statistics, including seismicity rates and b ‐values, should be treated with caution.

Kansas, Oklahoma↗

Uncertainty in ground-motion-to-intensity conversions significantly affects earthquake early warning alert regions

We examine how the choice of ground‐motion‐to‐intensity conversion equations (GMICEs) in earthquake early warning (EEW) systems affects resulting alert regions. We find that existing GMICEs can underestimate observed shaking at short rupture distances or overestimate the extent of low‐intensity shaking. Updated GMICEs that remove these biases would improve the accuracy of alert regions for the ShakeAlert EEW system for the West Coast of the United States. ShakeAlert uses ground‐motion prediction equations (GMPEs), which calculate spatial distributions of peak ground acceleration (PGA) and peak ground velocity (PGV) from earthquake source estimates, combined with GMICEs to translate GMPE output into modified Mercalli intensity (MMI). We find significant epistemic uncertainty in alert distances; near‐source MMI estimates from different GMICEs can differ by over 1 MMI unit, and MMI extents used for public EEW alerts can differ by hundreds of kilometers for larger magnitude earthquakes ( M ∼6.5+). We use a catalog of “Did You Feel It?” shaking reports to evaluate how well GMICEs predict observed shaking. Our preferred GMICE is the one that computes MMI using PGV for high intensities and transitions to using PGA for nondamaging intensities. These results motivate updating GMICE relationships more generally, including in ShakeMap applications.

The Seismic Record↗

Conversion factors: SI metric and U.S. customary units

The policy of the U.S. Geological Survey is to foster use of the International System of Units (SI) which was defined by the 11th General Conference of Weights and Measures in 1960. This modernized metric system constitutes an international "language" by means of which communications throughout the world's scientific and economic communities may be improved. This publication is designed to familiarize the reader with the SI units of measurement that correspond to the common units frequently used in programs of the Geological Survey. In the near future, SI units will be used exclusively in most publications of the Survey; the conversion factors provided herein will help readers to obtain a "feel" for each unit and to "think metric."

General Information Product↗

libvaxdata: VAX data format conversion routines

libvaxdata provides a collection of routines for converting numeric data-integer and floating-point-to and from the formats used on a Digital Equipment Corporation1 (DEC) VAX 32-bit minicomputer (Brunner, 1991). Since the VAX numeric data formats are inherited from those used on a DEC PDP-11 16-bit minicomputer, these routines can be used to convert PDP-11 data as well. VAX numeric data formats are also the default data formats used on DEC Alpha 64-bit minicomputers running OpenVMS The libvaxdata routines are callable from Fortran or C. They require that the caller use two's-complement format for integer data and IEEE 754 format (ANSI/IEEE, 1985) for floating-point data. They also require that the 'natural' size of a C int type (integer) is 32 bits. That is the case for most modern 32-bit and 64-bit computer systems. Nevertheless, you may wish to consult the Fortran or C compiler documentation on your system to be sure. Some Fortran compilers support conversion of VAX numeric data on-the-fly when reading or writing unformatted files, either as a compiler option or a run-time I/O option. This feature may be easier to use than the libvaxdata routines. Consult the Fortran compiler documentation on your system to determine if this alternative is available to you. 1Later Compaq Computer Corporation, now Hewlett-Packard Company

Open-File Report↗

Conversion of the Twin Cities metropolitan area numerical ground-water-flow model from the Trescott-Larson computer code to the McDonald-Harbaugh computer code

A numerical model of ground-water flow in the Twin Cities metropolitan area was converted from the TrescottLarson computer code to the McDonald-Harbaugh computer code to facilitate current and future use of the model using up-to-date computer software and hardware. Differences exist between the two computer codes in how headdependent source-sink functions, including leaky rivers, springs and seepage faces (drains), and head-dependent flux boundaries, are formulated. Because of differences in the formulation and calculation of the conductance terms, the conductance values for the head-dependent source-sink functions from the Trescott-Larson Twin Cities model were multiplied by the area of the appropriate model cell for conversion to the McDonald-Harbaugh Twin Cities model. Leaky rivers were simulated using the river package in the McDonald-Harbaugh Twin Cities model, springs and seepage faces were simulated using the drain package, and head-dependent flux boundaries were simulated using the general-head boundary package. Hydraulic heads and flows in the aquifer system calculated by the McDonald-Harbaugh Twin Cities model were compared to those calculated by the Trescott-Larson Twin Cities model to verify that the results calculated by the two models are similar. Mean differences in calculated hydraulic heads for the drift, drift and St. Peter aquifer, and drift and Prairie du Chien-Jordan aquifer model layers ranged from 1.1 to 1.6 feet. Mean differences in calculated hydraulic heads for the drift and Ironton-Galesville aquifer, drift and Eau Claire confining unit, and drift and Mount Simon-Hinckley aquifer model layers were 0.4 feet or less. Large differences in calculated hydraulic heads at a few cells probably were due to differences in the solution methods used in the McDonald-Harbaugh and Trescott-Larson computer codes to calculate hydraulic heads at and near model layer boundaries. Differences in calculated flow rates for the McDonald-Harbaugh and Trescott-Larson Twin Cities models were less than 0.3 cubic feet per second for recharge and the head-dependent source-sink functions. Differences in calculated flow in and flow out of constant-head cells for each model layer for the two models ranged from about 2 cubic feet per second for the Mount Simon-Hinckley aquifer model layer to about 45 cubic feet per second for the Prairie du ChienJordan aquifer model layer. The differences between the net flow rates at constant-head cells calculated by the two models for each model layer were much smaller, ranging from 0.01 cubic feet per second for the Ironton-Galesville and Mount Simon-Hinckley aquifer model layers to 8.39 cubic feet per second for the St. Peter aquifer model layer. Differences in the calculated ground-water budgets for the two models were 5.3 percent for the total sources and 4.4 percent for the total sinks.

Minnesota↗