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Statistical inference from capture data on closed animal populations

The estimation of animal abundance is an important problem in both the theoretical and applied biological sciences. Serious work to develop estimation methods began during the 1950s, with a few attempts before that time. The literature on estimation methods has increased tremendously during the past 25 years (Cormack 1968, Seber 1973). However, in large part, the problem remains unsolved. Past efforts toward comprehensive and systematic estimation of density (D) or population size (N) have been inadequate, in general. While more than 200 papers have been published on the subject, one is generally left without a unified approach to the estimation of abundance of an animal population This situation is unfortunate because a number of pressing research problems require such information. In addition, a wide array of environmental assessment studies and biological inventory programs require the estimation of animal abundance. These needs have been further emphasized by the requirement for the preparation of Environmental Impact Statements imposed by the National Environmental Protection Act in 1970. This publication treats inference procedures for certain types of capture data on closed animal populations. This includes multiple capture-recapture studies (variously called capture-mark-recapture, mark-recapture, or tag-recapture studies) involving livetrapping techniques and removal studies involving kill traps or at least temporary removal of captured individuals during the study. Animals do not necessarily need to be physically trapped; visual sightings of marked animals and electrofishing studies also produce data suitable for the methods described in this monograph. To provide a frame of reference for what follows, we give an exampled of a capture-recapture experiment to estimate population size of small animals using live traps. The general field experiment is similar for all capture-recapture studies (a removal study is, of course, slightly different). A typical field experiment is the following: a number of traps are positioned in the area to be studied, say 144 traps in a 12 X 12 grid, 7 m apart. At the beginning of the study (j=1) a sample size of n 1 is taken from the population, the animals are tagged and marked for future identification, and then returned to the population, usually at the same point where they were trapped. After allowing time of the marked and unmarked animals to mix, a second sample (j=2, often the following day) or n 2 animals is then taken.the second sample normally contains both marked and unmarked animals. The unmarked animals are marked and all captured animals are released back into the population. This procedure continues for t periods where t ≥ 2. The animals should be marked in such a way that the capture-recapture history of each animal caught during the study is known. In practice, toes are often clipped to uniquely identify individual animals (Taber and Cowan 1969) or serially numbered tags are sometimes used on larger animals. Such capture studies are classified by 2 schemes that are directly related to what class of models are appropriate and what parameters can be estimated. The first classification addresses the subject of closure. Closure usually means the size of the population is constant over the priod of investigation, i.e., no recruitment (birth or immigration) or losses (death or emigration). This is a strong assumption and, of course, never completely true in a natural biological population. For greater generality, we define closure to mean there are no unknown changes to the initial population. In practice, this means known losses (trap death), or deliberate removals) do not violate our definition of closure. If the study is properly designed, closure can be met at least approximately. Open or nonclosed populations explicitly allow for one or more types of recruitment or losses to operate during the course of the experiment (Jolly 1965, Seber 1965, Robson 1969, Pollock 1975). Only closed populations will be considered in this monograph. The second classification depends on the type of data collected with 2 possibilities occurring (Pollock 1974, unpublished doctoral dissertation, Cornell University, Ithaca, New York): (1) only information on the recovery of marked animals is available for each sampling occasion, j, j=1, 2, ... t. (2) information on both marked and unmarked animals is available for each sampling occasion, j, j=1, 2, ... t. In case (1), population size (N) is not identifiable, however, other parameters can be estimated (Brownie et al. 1978). In case (2), N can be estimated using a wide variety of approaches depending upon what we wish to assume. Only case (2) will be dealt with here.

Wildlife Monographs↗

A decision framework for the management of established biological invasions

In some cases, managing an established invasive species may do more harm to an ecosystem than allowing the invader to persist. Given limited resources available to land managers and the realities of conservation triage, we recognized the need for systematic guidance for management decisions made at the “late end” of the invasion curve. We gathered an interdisciplinary group of experts and practitioners to address the question of “under what circumstances is the active management of an established aquatic invasive species warranted?” Our working group identified three key dimensions to this question: (1) the efficacy of available management options; (2) the net benefits of management actions weighed against the null scenario of no control; and (3) the socio-ecological context that defines management goals, a manager’s ability to achieve said goals, and perceptions of management outcomes. These considerations were used to structure a consensus decision tree that supports a multi-criteria approach to decision-making. Our approach promotes interdisciplinarity and systems thinking and emphasizes the need to consider costs and benefits comprehensively, for example by considering the persistence or reversibility of impacts from both the invasive species and from efforts to suppress or eradicate it.

Facets↗

Ecological values of shallow-water habitats: Implications for the restoration of disturbed ecosystems

A presumed value of shallow-habitat enhanced pelagic productivity derives from the principle that in nutrient-rich aquatic systems phytoplankton growth rate is controlled by light availability, which varies inversely with habitat depth. We measured a set of biological indicators across the gradient of habitat depth within the Sacramento–San Joaquin River Delta (California) to test the hypothesis that plankton biomass, production, and pelagic energy flow also vary systematically with habitat depth. Results showed that phytoplankton biomass and production were only weakly related to phytoplankton growth rates whereas other processes (transport, consumption) were important controls. Distribution of the invasive clam Corbicula fluminea was patchy, and heavily colonized habitats all supported low phytoplankton biomass and production and functioned as food sinks. Surplus primary production in shallow, uncolonized habitats provided potential subsidies to neighboring recipient habitats. Zooplankton in deeper habitats, where grazing exceeded phytoplankton production, were likely supported by significant fluxes of phytoplankton biomass from connected donor habitats. Our results provide three important lessons for ecosystem science: (a) in the absence of process measurements, derived indices provide valuable information to improve our mechanistic understanding of ecosystem function and to benefit adaptive management strategies; (b) the benefits of some ecosystem functions are displaced by water movements, so the value of individual habitat types can only be revealed through a regional perspective that includes connectedness among habitats; and (c) invasive species can act as overriding controls of habitat function, adding to the uncertainty of management outcomes.

Ecosystems↗

Are hotspots of evolutionary potential adequately protected in southern California?

Reserves are often designed to protect rare habitats, or "typical" exemplars of ecoregions and geomorphic provinces. This approach focuses on current patterns of organismal and ecosystem-level biodiversity, but typically ignores the evolutionary processes that control the gain and loss of biodiversity at these and other levels (e.g., genetic, ecological). In order to include evolutionary processes in conservation planning efforts, their spatial components must first be identified and mapped. We describe a GIS-based approach for explicitly mapping patterns of genetic divergence and diversity for multiple species (a "multi-species genetic landscape"). Using this approach, we analyzed mitochondrial DNA datasets from 21 vertebrate and invertebrate species in southern California to identify areas with common phylogeographic breaks and high intrapopulation diversity. The result is an evolutionary framework for southern California within which patterns of genetic diversity can be analyzed in the context of historical processes, future evolutionary potential and current reserve design. Our multi-species genetic landscapes pinpoint six hotspots where interpopulation genetic divergence is consistently high, five evolutionary hotspots within which genetic connectivity is high, and three hotspots where intrapopulation genetic diversity is high. These 14 hotspots can be grouped into eight geographic areas, of which five largely are unprotected at this time. The multi-species genetic landscape approach may provide an avenue to readily incorporate measures of evolutionary process into GIS-based systematic conservation assessment and land-use planning.

Biological Conservation↗

Ants as a measure of effectiveness of habitat conservation planning in southern California

In the United States multispecies habitat conservation plans were meant to be the solution to conflicts between economic development and protection of biological diversity. Although now widely applied, questions exist concerning the scientific credibility of the conservation planning process and effectiveness of the plans. We used ants to assess performance of one of the first regional conservation plans developed in the United States, the Orange County Central‐Coastal Natural Community Conservation Plan (NCCP), in meeting its broader conservation objectives of biodiversity and ecosystem‐level protection. We collected pitfall data on ants for over 3 years on 172 sites established across a network of conservation lands in coastal southern California. Although recovered native ant diversity for the study area was high, site‐occupancy models indicated the invasive and ecologically disruptive Argentine ant ( Linepithema humile ) was present at 29% of sites, and sites located within 200 m of urban and agricultural areas were more likely to have been invaded. Within invaded sites, native ants were largely displaced, and their median species richness declined by more than 60% compared with uninvaded sites. At the time of planning, 24% of the 15,133‐ha reserve system established by Orange County NCCP fell within 200 m of an urban or agricultural edge. With complete build out of lands surrounding the reserve, the proportion of the reserve system vulnerable to invasion will grow to 44%. Our data indicate that simply protecting designated areas from development is not enough. If habitat conservation plans are to fulfill their conservation promise of ecosystem‐level protection, a more‐integrated and systematic approach to the process of habitat conservation planning is needed .

California↗

Coral disease and health workshop: Coral histopathology II, July 12-14, 2005

The health and continued existence of coral reef ecosystems are threatened by an increasing array of environmental and anthropogenic impacts. Coral disease is one of the prominent causes of increased mortality among reefs globally, particularly in the Caribbean. Although over 40 different coral diseases and syndromes have been reported worldwide, only a few etiological agents have been confirmed; most pathogens remain unknown and the dynamics of disease transmission, pathogenicity and mortality are not understood. Causal relationships have been documented for only a few of the coral diseases, while new syndromes continue to emerge. Extensive field observations by coral biologists have provided substantial documentation of a plethora of new pathologies, but our understanding, however, has been limited to descriptions of gross lesions with names reflecting these observations (e.g., black band, white band, dark spot). To determine etiology, we must equip coral diseases scientists with basic biomedical knowledge and specialized training in areas such as histology, cell biology and pathology. Only through combining descriptive science with mechanistic science and employing the synthesis epizootiology provides will we be able to gain insight into causation and become equipped to handle the pending crisis. One of the critical challenges faced by coral disease researchers is to establish a framework to systematically study coral pathologies drawing from the field of diagnostic medicine and pathology and using generally accepted nomenclature. This process began in April 2004, with a workshop titled Coral Disease and Health Workshop: Developing Diagnostic Criteria co-convened by the Coral Disease and Health Consortium (CDHC), a working group organized under the auspices of the U.S. Coral Reef Task Force, and the International Registry for Coral Pathology (IRCP). The workshop was hosted by the U.S. Geological Survey, National Wildlife Health Center (NWHC) in Madison, Wisconsin and was focused on gross morphology and disease signs observed in the field. A resounding recommendation from the histopathologists participating in the workshop was the urgent need to develop diagnostic criteria that are suitable to move from gross observations to morphological diagnoses based on evaluation of microscopic anatomy. As a continuation of building the foundation and framework for coral disease diagnostics, the CDHC convened the Coral Disease and Health Workshop: Coral Histopathology II in Charleston, South Carolina, July 11-14, 2005. The workshop was hosted by the Department of Pathology and Laboratory Medicine at the Medical University of South Carolina, Charleston, SC which provided expertise, facilities and equipment in support of the workshop. All of the histological slides and related photographs used in the discussions were prepared and supplied by the IRCP. This workshop brought together 15 experts in veterinary and medical pathology and coral biology from national and international research institutes and government laboratories. The mission was to devise a standardized approach to examining microscopic anatomy and pathology of corals and a standardized nomenclature to facilitate accurate descriptions of the microscopic morphology of corals and enhance communication among specialists investigating causes of coral death. 2 The participants of this workshop deliberated for 3 days to refine the nomenclature for gross and microscopic anatomy of corals and systematically described microscopic changes associated with selected coral diseases. The findings and recommendations from the deliberations will be submitted to the research community for peer review. The standardized nomenclature and descriptions produced at this workshop will ultimately be made available to the scientific community through a variety of media including the World Wide Web. An exciting highlight of this meeting was provided by Professor Robert Ogilvie (MUSC Department of Cell Biology and Anatomy) when he introduced participants to a new digital technology that is revolutionizing histology and histopathology in the medical field. The Virtual Slide technology creates digital images of histological tissue sections by computer scanning actual slides in high definition and storing the images for retrieval and viewing. Virtual slides now allow any investigator with access to a computer and the web to view, search, annotate and comment on the same tissue sections in real time. Medical and veterinary slide libraries across the country are being converted into virtual slides to enhance biomedical education, research and diagnosis. The coral health and disease researchers at this workshop deem virtual slides as a significant way to increase capabilities in coral histology and a means for pathology consultations on coral disease cases on a global scale.

NOAA Technical Memorandum↗

A citywide breeding bird survey for Washington, D.C.

`DC Birdscape' was initiated in 1993 to systematically count the birds occurring throughout Washington D.C. during the breeding season. It involved a coordinated planning effort and partnership between the Audubon Naturalist Society, the National Park Service, and the National Biological Survey, and engaged the participation of more than 100 volunteers. A method for rapidly assessing the status of bird populations over a large area was developed and incorporated into a Geographic Information System to allow a multidimensional analysis of species presence and abundance across a variety of urban land use areas. A total of 91 species were observed, with an estimated total number of 115, making Washington D.C. almost as `bird rich' as nearby suburban counties. Data from the study clearly indicate that avian species are not randomly distributed throughout the Washington D.C. metropolitan area, and show affinity, at least in part, to some of the most broadly recognized land use patterns that are commonly used to zone and classify urban areas under development schemes. This study represents a prototype that will allow efficient and economical monitoring of urban bird populations.

Washington, D.C.↗

The U.S. Geological Survey Federal-State Cooperative Water- Resources Program: Fiscal Year 1988

The Federal-State Cooperative Program is a partnership between the U.S. Geological Survey and State and local agencies. It provides a balanced approach to the study and resolution of water-related problems and to acquiring hydrologic data. The principal program objectives are to: (1) collect, on a systematic basis, data needed for the continuing determination and evaluation of the quantity, quality, and use of the Nation's water resources, and (2) appraise the availability and the physical, chemical, and biological characteristics of surface and ground water through analytical and interpretive investigations. During fiscal year 1988, hydrologic data collection, interpretive investigations, and research were conducted by Geological Survey personnel in offices in every State, Puerto Rico, and several territories in cooperation with more than 1,000 local, State, and regional agencies. In fiscal year 1988, Federal funding of almost $60 million was matched by cooperating agencies, who also provided approximately $6 million unmatched for a total program of about $126 million. This amounted to more than 40 percent of the total funds for Geological Survey water-resources activities. This report presents examples of current (1988) investigations. It also lists about 250 water-resources investigations related to agricultural activities that the Geological Survey conducted from 1970 to 1988.

Open-File Report↗

U. S. Geological Survey Federal-State Cooperative Water-Resources Program Fiscal Year 1993

The Federal-State Cooperative Program is a part- nership between the U.S. Geological Survey and State and local agencies. It provides a balanced approach to the study and resolution of water- related problems and to acquiring hydrologic data. The principal program objectives are to: (1) collect, on a systematic basis, data needed for the continuing determination and evaluation of the quantity, quality, and use of the Nation's water resources, and (2) appraise the availa- bility and the physical, chemical, and biological characteristics of surface and ground water through analytical and interpretive investi- gations. During fiscal year 1993, hydrologic data collection, interpretive investigations, and research were conducted by Geological Survey personnel in offices in every State, Puerto Rico, and in several territories in cooperation with about 1,100 local, State, and regional agencies. In fiscal year 1993, Federal funding of $63.5 million was matched by cooperating agencies, which also provided almost $23 million unmatched for a total program of about $150 million. This amounted to nearly 40 percent of the total funds for Geological Survey water- resources activities. This report presents examples of current (1993) investigations. It also provides updated information on Cooperative Program investigations related to agricultural activities.

Open-File Report↗

U.S. Geological Survey Federal-State Cooperative Water-Resources Program fiscal year 1994

The Federal-State Cooperative Program is a major U.S. Geological Survey (USGS) activity for the collection, analysis, and reporting of information on the quantity, quality, and use of the Nation's water resources. The fundamental characteristic of the program is that most of the work is undertaken by the USGS through joint-funding agreements, with State, regional, and local agencies providing at least one-half the funds. The main objectives of the program are (1) to collect, on a systematic basis, data needed for the continuing determination and evaluation of the quantity, quality, and use of the Nation's water resources; and (2) to appraise the availability and the physical, chemical, and biological characteristics of surface and ground water through data analysis and interpretive water-resources investigations and research. During fiscal year (FY)1994, Cooperative Program activities were underway in offices in every State, Puerto Rico, and several territories in concert with about 1,100 cooperating agencies. In FY 1994, Federal funding of $63.5 million was matched by cooperating agencies, which also provided more than $25 million unmatched for a total program of about $152 million. This amounted to nearly 40 percent of the total funds for the USGS's water-resources activities. This report presents examples of FY 1994 investigations, as well as updated information on hydrologic data collection activities.

Open-File Report↗

Geospatial characteristics of Florida's coastal and offshore environments: Coastal habitats, artificial reefs, wrecks, dumping grounds, harbor obstructions and offshore sand resources

The Geospatial Characteristics GeoPDF of Florida's Coastal and Offshore Environments is a comprehensive collection of geospatial data describing the political boundaries and natural resources of Florida. This interactive map provides spatial information on bathymetry, sand resources, coastal habitats, artificial reefs, shipwrecks, dumping grounds, and harbor obstructions. The map should be useful to coastal resource managers and others interested in marine habitats and submerged obstructions of Florida's coastal region. In particular, as oil and gas explorations continue to expand, the map may be used to explore information regarding sensitive areas and resources in the State of Florida. Users of this geospatial database will have access to synthesized information in a variety of scientific disciplines concerning Florida's coastal zone. This powerful tool provides a one-stop assembly of data that can be tailored to fit the needs of many natural resource managers. The map was originally developed to assist the Bureau of Ocean Energy Management, Regulation, and Enforcement (BOEMRE) and coastal resources managers with planning beach restoration projects. The BOEMRE uses a systematic approach in planning the development of submerged lands of the Continental Shelf seaward of Florida's territorial waters. Such development could affect the environment. BOEMRE is required to ascertain the existing physical, biological, and socioeconomic conditions of the submerged lands and estimate the impact of developing these lands. Data sources included the National Oceanic and Atmospheric Administration, BOEMRE, Florida Department of Environmental Protection, Florida Geographic Data Library, Florida Fish and Wildlife Conservation Commission, Florida Natural Areas Inventory, and the State of Florida, Bureau of Archeological Research. Federal Geographic Data Committee (FGDC) compliant metadata are provided as attached xml files for all geographic information system (GIS) layers.

Florida↗

U.S. Geological Survey federal-state cooperative water-resources program, fiscal year 1995

The Federal-State Cooperative Program is a major U.S. Geological Survey (USGS) activity for the collection, analysis, and reporting of information on the quantity, quality, and use of the Nation's water resources. The fundamental characteristic of the program is that most of the work is undertaken by the USGS through joint-funding agreements, with State, regional, and local agencies providing at least one-half the funds. The main objectives of the program are (1) to collect, on a systematic basis, data needed for the continuing determi- nation and evaluation of the quantity, quality, and use of the Nation's water resources; and (2) to appraise the availability and the physical, chemical, and biological characteristics of surface and ground water through data analysis and interpretive water-resources investigations and research. During fiscal year (FY) 1995, Cooperative Program activities were underway in offices in every State, Puerto Rico, and several territories in concert with about 1,100 cooperating agencies. In FY 1995, Federal funding of $62.1 million as matched by cooperating agencies, which also provided more than $28.2 million unmatched for a total program of about $152 million. This amounted to nearly 38 percent of the total funds for the USGS's water-resources activities. This report presents examples of FY 1995 investigations, as well as information on hydrologic data collection and water-use activities.

Open-File Report↗

Oxygen and sulfur isotope systematics of sulfate produced during abiotic and bacterial oxidation of sphalerite and elemental sulfur

Studies of metal sulfide oxidation in acid mine drainage (AMD) systems have primarily focused on pyrite oxidation, although acid soluble sulfides (e.g., ZnS) are predominantly responsible for the release of toxic metals. We conducted a series of biological and abiotic laboratory oxidation experiments with pure and Fe-bearing sphalerite (ZnS & Zn 0.88 Fe 0.12 S), respectively, in order to better understand the effects of sulfide mineralogy and associated biogeochemical controls of oxidation on the resultant δ 34 S and δ 18 O values of the sulfate produced. The minerals were incubated in the presence and absence of Acidithiobacillus ferrooxidans at an initial solution pH of 3 and with water of varying δ 18 O values to determine the relative contributions of H 2 O-derived and O 2 -derived oxygen in the newly formed sulfate. . Experiments were conducted under aerobic and anaerobic conditions using O 2 and Fe(III) aq as the oxidants, respectively. Aerobic incubations with A. ferrooxidans , and S o as the sole energy source were also conducted. The δ 34 S SO 4 "> δ34SSO4 values from both the biological and abiotic oxidation of ZnS and ZnS Fe by Fe(III) aq produced sulfur isotope fractionations ( ε 34 S SO 4 - ZnS "> ε34SSO4-ZnS ) of up to −2.6‰, suggesting the accumulation of sulfur intermediates during incomplete oxidation of the sulfide. No significant sulfur isotope fractionation was observed from any of the aerobic experiments. Negative sulfur isotope enrichment factors ( ε 34 S SO 4 - ZnS "> ε34SSO4-ZnS ) in AMD systems could reflect anaerobic, rather than aerobic pathways of oxidation. During the biological and abiotic oxidation of ZnS and ZnS Fe by Fe(III) aq all of the sulfate oxygen was derived from water, with measured ε 18 O SO 4 - H 2 O "> ε18OSO4-H2O values of 8.2 ± 0.2‰ and 7.5 ± 0.1‰, respectively. Also, during the aerobic oxidation of ZnS Fe and S o by A . ferrooxidans , all of the sulfate oxygen was derived from water with similar measured ε 18 O SO 4 - H 2 O "> ε18OSO4-H2O values of 8.1 ± 0.1‰ and 8.3 ± 0.3‰, respectively. During biological oxidation of ZnS by O 2 , an estimated 8% of sulfate–oxygen was derived from O 2 , which is enriched in 18 O relative to water, thus resulting in a larger apparent ε 18 O SO 4 - H 2 O "> ε18OSO4-H2O value of 9.5‰. Based on the data presented we hypothesize that the similar ε 18 O SO 4 - H 2 O "> ε18OSO4-H2O values of ∼8‰ from all of the aerobic and anaerobic experiments result from a common rate-limiting step that involves oxygen isotopic exchange between a sulfite ( SO 3 - "> SO3- ) intermediate and H 2 O. Our results indicate that the δ 18 O SO 4 "> δ18OSO4 values cannot be used to distinguish biological and abiotic, nor aerobic versus anaerobic, pathways of sphalerite oxidation. However, the ε 18 O SO 4 - H 2 O "> ε18OSO4-H2O values of ∼8‰ measured here are distinctly higher than ε 18 O SO 4 - H 2 O "> ε18OSO4-H2O values of ∼4‰ previously reported for pyrite oxidation indicating the influence of sulfide mineralogy on measured δ 18 O SO 4 "> δ18OSO4 values.

Geochimica et Cosmochimica Acta↗

Book review: Proceedings of the First International Snakehead Symposium

Snakehead fishes (family Channidae) are among the most maligned aquatic invasive species in the USA and some other countries where they have been introduced outside of their native range in Asia and Africa. Nevertheless, snakeheads continue to be widely exploited in the live‐food trade in aquaculture and wild‐capture fisheries, are highly sought by anglers, and are also popular in the aquarium trade (Courtenay and Williams 2004). The Northern Snakehead Channa argus is the most widespread of the three channid species that are currently naturalized in the USA. This species has generated much concern and controversy, a situation that is partly fueled by sensational media coverage and B‐grade science fiction horror films, such as “Frankenfish,” “Snakehead Terror,” and “Snakehead Swamp.” Media reports of snakehead introductions are often replete with provocative terms, such as “vicious,” “villain,” “voracious,” “monster,” “diabolical,” and even “ecological Armageddon.” When snakeheads first appeared in natural waters of the USA, fisheries professionals became increasingly interested in their status. Established populations rapidly expanded in the mid‐Atlantic region and Arkansas, with scattered reports of introduced snakeheads from isolated locations in Hawaii, California, North Carolina, Florida, the Upper Midwest, and New England. In 2002, snakeheads were added to the list of injurious fishes under the Lacey Act, thereby prohibiting their importation or transport across state lines without a permit. This symposium was conceived by the editors and other concerned fisheries professionals of the Mississippi River Basin Panel on Aquatic Invasive Species. The mission of the symposium, held in Alexandria, Virginia, in July 2018, was to bring together experts on snakehead biology and ecology and to synthesize existing information into summary papers. In this book, 35 authors contributed to 15 peer‐reviewed articles that detail the current state of knowledge about snakehead introductions in the USA. Additionally, 16 abstracts are included from meeting presentations that were not accompanied by full‐length manuscripts. Also included is a summary of a facilitated symposium panel discussion featuring eight experts representing state and federal natural resource agencies and private fishing organizations. The book is organized into six sections. In the first section (Distribution), three papers provide an overview of the Channa species introduced into the USA and historical accounts of occurrence and dispersal of the Northern Snakehead in the mid‐Atlantic region and Arkansas. The second section (Biology/Ecology) consists of two articles that examine growth and energetics of Northern Snakehead populations and two papers that investigate diet, diel feeding activity, and movement of this species in the Potomac River drainage. The third section (Monitoring/Response) includes a paper that models range expansion of the Northern Snakehead in the southeastern USA based on occurrence data and environmental conditions. Also included in this section is a paper summarizing an environmental DNA study to assess the status and range of the Bullseye Snakehead C. marulius in southern Florida. The fourth section (Management/Control) is comprised of four papers that address harvest, age and growth, and development of a stock–recruitment model to inform management decisions regarding control and mitigation for Northern Snakehead populations in the greater Chesapeake Bay area. The fifth section (Perspectives) includes a paper on the history of snakehead introductions in Japan and a thought‐provoking social commentary on the human dimensions of Northern Snakehead management. Abstracts in the final section provide brief summaries of a diversity of snakehead studies, including aspects of distribution, ecology, behavior, control and monitoring efforts, public outreach, and pathology. The summary of the panel discussion is an engaging dialogue about the challenges of snakehead management in the context of conflicts regarding snakeheads as injurious versus their value as game and food species. Most of this book is focused on the Northern Snakehead. Much has been done to document snakehead distributions and certain aspects of snakehead biology, such as diets, age, and growth. Less research has been devoted to understanding the ecological impacts of snakeheads to native aquatic communities and ecosystems. This book would have benefited from a chapter summarizing the current systematics and diversity of the Channidae to inform fisheries biologists about the morphological characteristics of the family, approximate numbers of genera and species, and taxonomic instability. Exemplifying the latter, recent molecular and morphological evidence indicates uncertainty regarding identification of the feral snakehead population in Florida (Adamson and Britz 2019). Those authors suggest that this population may have originated from western Thailand, a possibility that could have implications for understanding historical pathways of snakehead introductions into the USA. In comparison with many published AFS symposia, this volume is relatively narrow in scope and lacks cohesive integration. It will primarily be of interest to those fisheries professionals engaged in the study of snakeheads as well as other nonnative species for which there are contrasting social values regarding their management: whether to monitor and attempt control or eradication efforts or to maintain populations for harvest as game or food species. The book should serve to identify information gaps and guide future research.

Transactions of the American Fisheries Society↗

Oxygen and sulfur isotope systematics of sulfate produced by bacterial and abiotic oxidation of pyrite

To better understand reaction pathways of pyrite oxidation and biogeochemical controls on ??18O and ??34S values of the generated sulfate in acid mine drainage (AMD) and other natural environments, we conducted a series of pyrite oxidation experiments in the laboratory. Our biological and abiotic experiments were conducted under aerobic conditions by using O2 as an oxidizing agent and under anaerobic conditions by using dissolved Fe(III)aq as an oxidant with varying ??18OH2O values in the presence and absence of Acidithiobacillus ferrooxidans. In addition, aerobic biological experiments were designed as short- and long-term experiments where the final pH was controlled at ???2.7 and 2.2, respectively. Due to the slower kinetics of abiotic sulfide oxidation, the aerobic abiotic experiments were only conducted as long term with a final pH of ???2.7. The ??34SSO4 values from both the biological and abiotic anaerobic experiments indicated a small but significant sulfur isotope fractionation (???-0.7???) in contrast to no significant fractionation observed from any of the aerobic experiments. Relative percentages of the incorporation of water-derived oxygen and dissolved oxygen (O2) to sulfate were estimated, in addition to the oxygen isotope fractionation between sulfate and water, and dissolved oxygen. As expected, during the biological and abiotic anaerobic experiments all of the sulfate oxygen was derived from water. The percentage incorporation of water-derived oxygen into sulfate during the oxidation experiments by O2 varied with longer incubation and lower pH, but not due to the presence or absence of bacteria. These percentages were estimated as 85%, 92% and 87% from the short-term biological, long-term biological and abiotic control experiments, respectively. An oxygen isotope fractionation effect between sulfate and water (??18 OSO4 s(-) H2 O) of ???3.5??? was determined for the anaerobic (biological and abiotic) experiments. This measured ??18 OSO42 - s(-) H2 O value was then used to estimate the oxygen isotope fractionation effects (??18 OSO42 - s(-) O2) between sulfate and dissolved oxygen in the aerobic experiments which were -10.0???, -10.8???, and -9.8??? for the short-term biological, long-term biological and abiotic control experiments, respectively. Based on the similarity between ??18OSO4 values in the biological and abiotic experiments, it is suggested that ??18OSO4 values cannot be used to distinguish biological and abiotic mechanisms of pyrite oxidation. The results presented here suggest that Fe(III)aq is the primary oxidant for pyrite at pH < 3, even in the presence of dissolved oxygen, and that the main oxygen source of sulfate is water-oxygen under both aerobic and anaerobic conditions. ?? 2007 Elsevier Ltd. All rights reserved.

Geochimica et Cosmochimica Acta↗

Mismatch between conservation status and climate change sensitivity leaves some anurans in the United States unprotected

Species vulnerable to climate change face increased extinction risk, but many sensitive species may be overlooked due to limited data and exclusion from vulnerability assessments. Intrinsic sensitivity, or the inherent risk of species to environmental change due to biological factors, can be assessed with widely available data and may address gaps in multispecies vulnerability assessments. Species that exist in few places (geographically rare) and in fewer climates (smaller realized climate niche breadth) have high intrinsic sensitivity to environmental change. Using point occurrences, we systematically evaluated intrinsic sensitivity based on geographic rarity and realized climate niche breadth for 90 species of frogs and toads native to the United States using over 140 000 occurrence records. To compare sensitivity to perceived extinction risk, we compared intrinsic sensitivity to conservation status at state, federal, and international levels. We found no relationship between intrinsic sensitivity and federal or state conservation status, with some intrinsically sensitive species (i.e., those with small areas of occurrence and narrow climate specificity) not listed as at-risk at any level. Intrinsic sensitivity analysis can serve as an early warning system for species that may be currently at-risk and overlooked.

Biological Conservation↗

Large-area mapping of biodiversity

The age of discovery, description, and classification of biodiversity is entering a new phase. In responding to the conservation imperative, we can now supplement the essential work of systematics with spatially explicit information on species and assemblages of species. This is possible because of recent conceptual, technical, and organizational progress in generating synoptic views of the earth's surface and a great deal of its biological content, at multiple scales of thematic as well as geographic resolution. The development of extensive spatial data on species distributions and vegetation types provides us with a framework for: (a) assessing what we know and where we know it at meso-scales, and (b) stratifying the biological universe so that higher-resolution surveys can be more efficiently implemented, coveting, for example, geographic adequacy of specimen collections, population abundance, reproductive success, and genetic dynamics. The land areas involved are very large, and the questions, such as resolution, scale, classification, and accuracy, are complex. In this paper, we provide examples from the United States Gap Analysis Program on the advantages and limitations of mapping the occurrence of terrestrial vertebrate species and dominant land-cover types over large areas as joint ventures and in multi-organizational partnerships, and how these cooperative efforts can be designed to implement results from data development and analyses as on-the-ground actions. Clearly, new frameworks for thinking about biogeographic information as well as organizational cooperation are needed if we are to have any hope of documenting the full range of species occurrences and ecological processes in ways meaningful to their management. The Gap Analysis experience provides one model for achieving these new frameworks.

Conference Paper↗