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Wind River subbasin restoration annual report of USGS activities January 2017 through December 2017

We used Passive Integrated Transponder (PIT)-tagging and a series of instream PIT-tag interrogation systems (PTISs) to investigate life-histories, populations, and efficacy of habitat restoration actions for wild Steelhead Oncorhynchus mykiss in the Wind River subbasin, WA. No hatchery Steelhead have been planted in the Wind River subbasin since 1997, and hatchery adults are estimated to be less than one percent of adults in most years (pers comm. Thomas Buehrens, Washington Department of Fish and Wildlife). Numerous restoration actions have been implemented in the subbasin, including Hemlock Dam removal on Trout Creek in 2009. Data from our study, and companion work by Washington Department of Fish and Wildlife (WDFW), are contributing to Bonneville Power Administration’s (BPA) Research Monitoring and Evaluation (RM&E) Program Strategy of Fish Population Status Monitoring (www.cbfish.org/ProgramStrategy.mvc/ViewProgramStrategySummary/1), specifically the substrategies of: 1) Assessing the Status and Trends of Diversity of Natural Origin Fish Populations and to Uncertainties Research regarding differing life histories of a wild Steelhead population, 2) Assessing the Status and Trend of Adult Natural Origin Fish Populations, and 3) Monitoring and Evaluating the Effectiveness of Tributary Habitat Actions Relative to Environmental, Physical, or Biological Performance Objectives. Our headwaters parr PIT tagging, WDFW parr, smolt, and adult tagging and our instream PTISs are providing data movements and life histories of parr, smolt, and adult Steelhead. During summer 2017, we PIT-tagged age-0 and age-1 Steelhead parr in headwater areas of the Wind River subbasin to characterize population traits and investigate life-history diversity, including growth and pre-smolt downstream movement. Repeat sampling and smolt traps provide opportunities for recapture, and instream PTISs and Columbia River infrastructure provide opportunity for detection of PIT-tagged fish. Throughout the year, we maintained a series of instream PTISs to monitor movement of tagged Steelhead parr, smolts, and adults. This included adding the second array to our upper Wind River PITS, increasing solar capacity and adding improved power cables to some sites. Detections at the instream PTISs have demonstrated trends of age-0 and age-1 parr emigration from natal areas during summer and fall, in addition to the expected movement of parr and smolts in spring. These data are increasing our understanding of varied life histories of juvenile Steelhead; paired with other Steelhead population work in the subbasin we hope to begin to understand factors which may influence parr movements. Long-term monitoring of PIT-tagged fish over multiple years is providing information on contribution of various life-history strategies to smolt production and adult returns. Movements of PIT-tagged adult Steelhead were also recorded at instream PTISs. These data have allowed us to assess adult returns to tributary watersheds within the Wind River subbasin. Determination of adult use of tributary watersheds is providing data to contribute to evaluation of the efficacy of the removal of Hemlock Dam on Trout Creek. Hemlock Dam, located at rkm 2.0 of Trout Creek was removed in summer 2009. The dam had had contributed to hydrologic impairment of Trout Creek and had potential negative effects on Steelhead. The improved upper Wind River PTIS (better site characteristics and grid power) will allow estimates of subbasin adult escapement upstream of that site. Evaluating and planning restoration efforts are of interest to many managers and agencies to ensure efficient use of resources. The evaluation of various life-histories of Lower Columbia River Steelhead within the Wind River subbasin will provide information to better track populations, and to direct habitat restoration and water allocation planning. Increasingly detailed Viable Salmonid Population information, such as that provided by PIT-tagging and instream PTISs networks like those we operate in the Wind River subbasin, will provide data to inform policy and management, as life-history strategies and production bottlenecks are identified and understood.

Washington↗

Attitudinal survey component of the study Quantity, quality, and support for research in the U.S. Fish and Wildlife Service: An organizational assessment : Report of methods and frequencies

The U.S. Fish and Wildlife Service (FWS) is responsible for managing the Nation’s fish and wildlife resources so that these trust resources are preserved for the present and future use and enjoyment of the citizens of the United States. The FWS achieves this mission by managing many programs. These include the national system of refuges and fish hatcheries, Fish and Wildlife Management Assistance Offices, migratory birds program, law enforcement, and working with tribal, state, and other Federal agencies to ensure protection of threatened and endangered species. Another role of the FWS is consulting with tribal, state, and other Federal agencies and private sector interests on the best conservation management practices consistent with Federal law. Each of these activities requires a workforce that is recognized for its professionalism, dedication to public service, and command of expert knowledge. Recognition for expert knowledge in fish and wildlife conservation is demonstrated, in part, when FWS personnel direct, conduct, or report research that is well-designed to answer questions of importance for natural resource management. The data reported in this document are one part of a three-part study of the status of organizational support for research in FWS, which was commissioned by the Directorate of the FWS. Funding for this study was provided by the FWS, and the Science Support Program of the U.S. Geological Survey (USGS). In 1994, the biological research functions of the FWS were transferred to the National Biological Survey, and subsequently into the USGS. This transfer was principally accomplished by moving whole research units from one agency to another. The result was that some employees whose positions were involved with research were not transferred. In addition, some research, information, and management needs of the FWS have continued to be met by studies conducted within the FWS itself. Although the FWS relies on the USGS and others for most basic research investigations, the FWS also conducts its own studies to meet management needs. Because it is vital for FWS employees to be able to conduct such tactical and applied research tasks in a timely manner, the agency must promote the culture necessary to support and encourage these activities. Such research activities are spread widely across the various programs of the FWS, and there is presently no collective, formal, or systematic record of planned or existing research activities. In commissioning this organizational assessment research, the Directorate of the FWS recognized that it would be to the advantage of the agency to more fully understand its research capacity. To develop a clearer picture of the nature, extent, quality, and degree of administrative support available for conducting research within the FWS, investigations have been undertaken to: 1. identify positions in the FWS that may include, in whole or in part, a component of scientific research; 2. identify organizational units within the FWS that may conduct research as a significant portion of their mission; and 3. assess the attitudes of employees and managers about the obstacles and opportunities for scientific research existing within the FWS by using a scaled-response survey instrument. The findings presented in this report represent the basic results derived from the attitude assessment survey conducted in the last quarter of 2004. The findings set forth in this report are the frequency distributions for each question in the survey instrument for all respondents. The only statistics provided are descriptive in character - namely, means and associated standard deviations.

Open-File Report↗

Reconnaissance investigation of water quality, bottom sediment, and biota associated with irrigation drainage in the Vermejo Project area and the Maxwell National Wildlife Refuge, Colfax County, northeastern New Mexico, 1993

Based on findings of limited studies during 1989-92, a reconnaissance investigation was conducted in 1993 to assess the effects of the Vermejo Irrigation Project on water quality in the area of the project, including the Maxwell National Wildlife Refuge. This project was part of a U.S. Department of the Interior National Irrigation Water-Quality Program to determine whether irrigation drainage has caused or has the potential to cause significant harmful effects on human health, fish, and wildlife and whether irrigation drainage may adversely affect the suitability of water for other beneficial uses. For this study, samples of water, sediment, and biota were collected from 16 sites in and around the Vermejo Irrigation Project prior to, during the latter part of, and after the 1993 irrigation season (April, August-September, and November, respectively). No inorganic constituents exceeded U.S. Environmental Protection Agency drinking-water standards. The State of New Mexico standard of 750 micrograms per liter for boron in irrigation water was exceeded at three sites (five samples), though none exceeded the livestock water standard of 5,000 micrograms per liter. Selenium concentrations exceeded the State of New Mexico chronic standard of 2 micrograms per liter for wildlife and fisheries water in at least eight samples from five sites. Bottom-sediment samples were collected and analyzed for trace elements and compared to concentrations of trace elements in soils of the Western United States. Concentrations of three trace elements at eight sites exceeded the upper values of the expected 95-percent ranges for Western U.S. soils. These included molybdenum at one site, selenium at seven sites, and uranium at four sites. Cadmium and copper concentrations exceeded the National Contaminant Biomonitoring Program 85th percentile in fish from six sites. Average concentrations of selenium in adult brine flies (33.7 μ g/g dry weight) were elevated above concentrations in other invertebrates. Concentrations of other elements were below their respective toxicity levels. Plants, invertebrates, fish, and fish fillets were collected and analyzed. These analyses were compared to diagnostic criteria and to each other to determine the extent of bioaccumulation of trace elements. Plants contained larger dry weight concentrations of aluminum, arsenic, boron, chromium, iron, lead, magnesium, manganese, nickel, and vanadium than invertebrates and fish. Adult brine flies, gathered from playas, contained larger geometric mean dry weight concentrations of boron, magnesium, and selenium than other invertebrates. Of all samples collected, the largest mercury concentrations were found in fish fillets, although these concentrations were below levels of concern. Mercury and selenium bioaccumulation was evident in various habitats of the study area. Biological samples from Natural playa, an endemic wetland, and Half playa, a playa that receives additional water through seepage and irrigation delivery canals, generally had elevated concentrations of boron, iron, magnesium, and selenium than samples from reservoir and river sites. Selenium concentrations were lowest in biota from the two reservoir sites, although a wetland immediately downstream from the dam impounding Lake No. 13 (created by seepage from the reservoir) had elevated concentrations of selenium in biota. The geometric mean selenium concentration of whole-fish samples, except those from Lakes No. 13 and No. 14, exceeded the 5- μ g/g dry weight selenium concentration that demarcates the approximate lower limit of the threshold range of concentrations that have freen associated with adverse effects on piscine reproduction. Biota collected on and in the area around Maxwell National Wildlife Refuge contained concentrations of selenium that are in the lower range of threshold values that have been associated (dietarily) with risks of avian reproductive abnormalities. Bottom-sediment samples from two sites downstream from the irrigation project were collected and analyzed for 23 organic compounds (principally organochlorine pesticide residues). Three compounds were detected at the two sites: DDD was found at 0.2 microgram per kilogram, DDE was detected at 0.1 microgram per kilogram at both sites, and chlordane was detected at 1 microgram per kilogram. None of the 28 whole-fish and fillet samples analyzed for PCB and organochlorine pesticide residues contained any of the 23 organic compounds tested for at concentrations higher than the analytical reporting limit (less than 0.01 μ g/g wet weight). Concentrations of inorganic analytes were generally within established guidelines or expected concentrations for water, sediment, and biota. lirigation-return flows were found to be unrelated to adverse effects in biota.

New Mexico↗

Spatial education: improving conservation delivery through space-structured decision making

Adaptive management is a form of structured decision making designed to guide management of natural resource systems when their behaviors are uncertain. Where decision making can be replicated across units of a landscape, learning can be accelerated, and biological processes can be understood in a larger spatial context. Broad-based partnerships among land management agencies, exemplified by Landscape Conservation Cooperatives (conservation partnerships created through the U.S. Department of the Interior), are potentially ideal environments for implementing spatially structured adaptive management programs.

Journal of Fish and Wildlife Management↗

Wind River subbasin restoration: U.S. Geological Survey annual report November 2012 through December 2013

Executive Summary The Wind River subbasin in southwest Washington State provides habitat for a population of wild Lower Columbia River steelhead Oncorhynchus mykiss . There have been no hatchery steelhead planted in the Wind River subbasin since 1994, and hatchery adults are estimated to be less than one percent of adults in any year (pers comm. Thomas Buehrens, Washington Department of Fish and Wilflife). We used Passive Integrated Transponder (PIT)-tagging and a series of instream PIT-tag interrogation systems (PTIS) to investigate life-histories, populations, and efficacy of habitat restoration actions for these steelhead. Data from our study, and companion work by Washington Department of Fish and Wildlife (WDFW), will contribute to Bonneville Power Administration’s (BPA) Research Monitoring and Evaluation (RM&E) Program Strategy of Fish Population Status Monitoring ( www.cbfish.org/ProgramStrategy.mvc/ViewProgramStrategySummary/1 ), specifically the sub-strategies of: 1) Assessing the Status and Trends of Diversity of Natural Origin Fish Populations and to uncertainties research regarding differing life histories of a wild steelhead population, 2) Assessing the Status and Trend of Adult Natural Origin Fish Populations, and 3) Monitoring and Evaluating the Effectiveness of Tributary Habitat Actions Relative to Environmental, Physical, or Biological Performance Objectives. During summer 2013, we PIT-tagged parr steelhead in headwater areas of the Wind River subbasin to investigate variable life-histories, specifically to compare fate of those juvenile steelhead that move downstream prior to smolting with those that remain in their natal areas until smolting. A series of instream PTISs monitored movement of these fish. Detections at the instream PTISs showed trends of parr emigration during summer and fall, in addition to the expected movement of parr and smolts in spring. Long-term monitoring of PIT-tagged fish over multiple years will provide information on contribution of various life-history strategies to smolt production and adult returns, as well as helping to identify factors influencing parr movement. Movements of PIT-tagged adult steelhead were tracked with our instream PTISs. These data have provided information on timing of adult movements to various parts of the watershed, which is allowing us to assess adult returns to tributary watersheds within the Wind River subbasin. Determination of adult use of tributary watersheds has provided data that will contribute to evaluating the efficacy of the removal of Hemlock Dam from Trout Creek. Hemlock Dam, located at rkm 2.0 of Trout Creek was removed in summer 2009 and had contributed to hydrologic impairment of Trout Creek. Evaluating restoration efforts is of interest to many managers and agencies so that funding and time are allocated for best results. The evaluation of various life-histories of Lower Columbia River steelhead within the Wind River subbasin will provide information to better track populations, and to direct habitat restoration and water allocation planning. Increasingly detailed Viable Salmonid Population information, such as that provided by PIT-tagging and instream PTISs networks like those we are building and operating in the Wind River subbasin, will provide data to inform policy and management, as life-history strategies and production bottlenecks are identified and understood.

Washington↗

Wind River subbasin restoration: Annual report of U.S. Geological Survey activities January 2014 through December 2014

Executive Summary The Wind River subbasin in southwest Washington State provides habitat for a population of wild Lower Columbia River steelhead Oncorhynchus mykiss , which are listed as threatened under the Endangered Species Act. No hatchery steelhead have been planted in the Wind River subbasin since 1994, and hatchery adults are estimated to be less than one percent of adults in any year (Thomas Buehrens, Washington Department of Fish and Wildlife, personal communication). Numerous restoration actions have been implemented in the subbasin, including the removal of Hemlock Dam on Trout Creek in 2009. We used Passive Integrated Transponder (PIT) tagging and a series of instream PIT-tag interrogation systems (PTIS) to investigate life-histories, populations, and efficacy of habitat restoration actions for these steelhead. Data from our study, and companion work by Washington Department of Fish and Wildlife (WDFW), will contribute to Bonneville Power Administration’s (BPA) Research Monitoring and Evaluation (RM&E) Program Strategy of Fish Population Status Monitoring ( www.cbfish.org/ProgramStrategy.mvc/ViewProgramStrategySummary/1 ), specifically the sub-strategies of: 1) Assessing the Status and Trends of Diversity of Natural Origin Fish Populations and to Uncertainties Research regarding differing life histories of a wild steelhead population, 2) Assessing the Status and Trend of Adult Natural Origin Fish Populations, and 3) Monitoring and Evaluating the Effectiveness of Tributary Habitat Actions Relative to Environmental, Physical, or Biological Performance Objectives. During summer 2014, we PIT-tagged steelhead parr in headwater areas of the Wind River subbasin to investigate life-history diversity, specifically to compare fate of those juvenile steelhead that move downstream prior to smolting with those that remain in their natal areas until smolting. A series of instream PTISs monitored movement of these fish. We added a new multi-antenna PTIS on Trout Creek and made improvements to two of our smaller tributary PTISs during 2014. Detections at the instream PTISs showed trends of parr emigration during summer and fall, in addition to the expected movement of parr and smolts in spring. Long-term monitoring of PIT-tagged fish will provide information on contribution of various life-history strategies to smolt production and adult returns, as well as helping to identify factors influencing parr movement. Movements of PIT-tagged adult steelhead were tracked with our instream PTISs. These data will contribute to a better understanding of timing and distribution of spawning by this population of wild steelhead within the Wind River subbasin. Additionally, these data have provided information on timing of adult movements to various parts of the watershed, which is allowing us to assess adult use of tributary watersheds within the Wind River subbasin. These data are contributing to evaluating steelhead response to the removal of Hemlock Dam from Trout Creek. Hemlock Dam, which was located at rkm 2.0 of Trout Creek, was removed in summer 2009 and had contributed to hydrologic impairment of Trout Creek and potentially caused some deterrent to upstream adult steelhead migration. Evaluating restoration efforts is of interest to many managers and agencies so that funding and time are allocated for best results. The evaluation of various life-histories of Lower Columbia River steelhead within the Wind River subbasin provides information to better track populations, and more effectively direct habitat restoration and water allocation planning. Increasingly detailed Viable Salmonid Population information (Crawford and Rumsey 2009), such as that provided by PIT-tagging and instream PTISs networks like those we build and operate in the Wind River subbasin, provide data to better inform policy and management, as life-history strategies and production bottlenecks are identified and understood.

Washington↗

Wind River Subbasin Restoration, annual report of U.S. Geological Survey activities: Parr monitoring and instream passive integrated transponder detection, January 1, 2015 – December 31, 2015

We used Passive Integrated Transponder (PIT)-tagging and a series of instream PIT-tag interrogation systems (PTIS) to investigate life-histories, populations, and efficacy of habitat restoration actions for steelhead Oncorhynchus mykiss in the Wind River subbasin, WA. Our tagging focused on parr in headwater areas of the subbasin and our PTISs provide information on movement of these parr, which is primarily, but not exclusively downstream. The PTISs also provide data on life-history aspects of other steelhead life-stages. The Wind River subbasin in southwest Washington State provides habitat for a population of wild Lower Columbia River steelhead and is an excellent watershed for long-term studies of population dynamics and responses to restoration of this wild population. Much data on steelhead population metrics have been gathered from the Wind River providing information on habitat restoration actions and ongoing research into steelhead life histories. Additionally, the Wind River is an excellent control watershed of an exclusivly wild steelhead population to which basins with hatchery programs can compare. No hatchery steelhead have been planted in the Wind River subbasin since 1994, and hatchery adults are estimated to be less than one percent of adults in any year (pers comm. Thomas Buehrens, Washington Department of Fish and Wildlife). Numerous restoration actions have been implemented in the subbasin, including the removal of Hemlock Dam on Trout Creek in 2009. Data from our study, and companion work by Washington Department of Fish and Wildlife (WDFW), will contribute to Bonneville Power Administration’s (BPA) Research Monitoring and Evaluation (RM&E) Program Strategy of Fish Population Status Monitoring (www.cbfish.org/ProgramStrategy.mvc/ViewProgramStrategySummary/1), specifically the sub-strategies of: 1) Assessing the Status and Trends of Diversity of Natural Origin Fish Populations and to uncertainties research regarding differing life histories of a wild steelhead population, 2) Assessing the Status and Trend of Adult Natural Origin Fish Populations, and 3) Monitoring and Evaluating the Effectiveness of Tributary Habitat Actions Relative to Environmental, Physical, or Biological Performance Objectives. During summer 2015, we sampled and PIT-tagged age-0 and age-1 steelhead parr in headwater areas of the Wind River subbasin to characterize population traits and investigate variable life-histories, including growth and parr movement downstream prior to smolting. Throughout the year, we maintained a series of instream PTISs to monitor movement of tagged steelhead parr, smolts, and adults. Detections at the instream PTISs showed trends of parr 8 emigration during summer and fall, in addition to the expected movement of parr and smolts in spring. These data are increasing our understanding of varied life histories of juvenile steelhead; paired with other steelhead population work in the subbasin we hope to better understand the factors influencing parr movements. Monitoring of PIT-tagged fish over multiple years is providing information on contribution of various life-history strategies to smolt production and adult returns, as well as identifying factors influencing parr movement. Movements of PIT-tagged adult steelhead were also monitored with our instream PTISs. These data have provided information on timing of adult movements to various parts of the watershed, which allows us to assess adult returns to tributary watersheds within the Wind River subbasin. Determination of adult use of tributary watersheds is providing data to contribute to evaluation of the efficacy of the removal of Hemlock Dam on Trout Creek. Hemlock Dam, located at rkm 2.0 of Trout Creek was removed in summer 2009 and had contributed to hydrologic impairment of Trout Creek. Evaluating restoration efforts is of interest to many managers and agencies so that funding and time are allocated for best results. The evaluation of various life-his

Washington↗

Wind River subbasin restoration: Annual Report of U.S. Geological Survey activities January 2020 through December 2020

We sampled juvenile wild Steelhead Trout Oncorhynchus mykiss in headwater streams of the Wind River, WA, to characterize population attributes and investigate life-history metrics, particularly migratory patterns, and early life-stage survival. We used passive integrated transponder (PIT) tagging and a series of instream PIT-tag interrogation systems (PTISs) to track juveniles and adults. The Wind River subbasin is considered a wild Steelhead refuge by Washington Department of Fish and Wildlife (WDFW). No hatchery Steelhead Trout have been released in the Wind River subbasin since 1997, and hatchery adults are estimated at less than one percent of spawners in most years. Over twenty years of Steelhead Trout status and trend monitoring and research in the subbasin is contributing to understanding of population response to numerous restoration actions in the subbasin, including removal of Hemlock Dam from Trout Creek in 2009, which had an outdated adult ladder and contributed to increased water temperatures reducing performance of juvenile Steelhead Trout. Data from our study, and companion work by Washington Department of Fish and Wildlife, are contributing to Bonneville Power Administration’s (BPA) Research, Monitoring, and Evaluation (RM&E) Program Strategy of Fish Population Status Monitoring (https://www.cbfish.org/ProgramStrategy.mvc/Index). Specifically, this work addresses the substrategies of 1) Assessing the Status and Trends of Diversity of Natural Origin Fish Populations and Uncertainties Research regarding differing life histories of a wild Steelhead Trout population, 2) Assessing the Status and Trend of Adult Natural Origin Fish Populations, and 3) Monitoring and Evaluating the Effectiveness of Tributary Habitat Actions Relative to Environmental, Physical, or Biological Performance Objectives. During summer and fall 2020, we PIT-tagged 1,415 Steelhead parr (age-0 and age-1) in the Trout Creek and upper Wind River watersheds. Recaptures and detections of PIT-tagged Steelhead Trout parr happened through repeat headwater sampling, smolt trap operations, and instream PTISs and Columbia River PIT-tag detection infrastructure. Throughout the year, we maintained a series of six instream PTISs to monitor movement of tagged Steelhead Trout parr, smolts, and adults, providing data to population assessments, and life-cycle research and modeling. Detection data from PIT-tagged adult Steelhead Trout at PTISs allow assessment of adult escapement to tributary watersheds within the Wind River subbasin. Adult Steelhead Trout detection efficiency estimates at our primary PTIS in Trout Creek have been greater than 92 percent during eight of the past nine years and have exceeded 90% at our primary PTIS in the Wind River the past three years. Adult escapement estimates to tributary watersheds are helping evaluate the efficacy of the 2009 removal of Hemlock Dam from rkm 2.0 of Trout Creek. The dam had potential negative effects on Steelhead Trout populations in Trout Creek due to hydrologic impairment, increased temperatures, and adult passage issues. Hemlock Dam was laddered for adult passage, but not to modern standards, which likely resulted in avoidance by some adult Steelhead Trout. We continue to improve our PTISs in the Wind River subbasin. The improvements in siting and addition of grid power to the upper Wind River PTIS (site code WRU, rkm 27.6) during 2016 and 2017, and the addition of the Mine Reach site (site code MIN, rkm 36.0) have much improved PIT-tagged fish monitoring in the upper Wind River watershed. The paired PTIS design in the upper Wind River watershed (sites WRU and MIN), matches that in the Trout Creek watershed (sites TRC and TC4) and will allow comparisons of Steelhead Trout population metrics between the two watersheds as response to Hemlock Dam removal continues and future restoration efforts occur in Trout Creek. We installed two new PTISs during 2020. Both were installed downstream of our primary interrogation sites on Trout Creek and in the mainstem Wind River. We hope the two new sites will provide interrogations information that will allow us to better estimate detection efficiencies of downstream moving juvenile Steelhead Trout at the primary interrogation sites. The additional interrogations will be particularly important for those fish tagged with 9-mm PIT tags as less information from downstream locations is available from them. These sites and other status and trend data will allow evaluation of further planned restoration within the watershed, particularly that proposed for the headwaters of Trout Creek. Detections at the instream PTISs have demonstrated trends of age-0 and age-1 parr emigration from natal areas during summer and fall, in addition to the expected movement of parr and smolts in spring. We have estimated that from 15 to 51% of parr tagged as age-0 fish in headwater areas make downstream migrations at age 1 for additional rearing during both spring and fall. We have estimated that up to 27% of Steelhead Trout parr, tagged as age-1 fish, make downstream migrations during fall. These findings raise questions about where parr most successfully rear and whether migrations are density or habitat quality driven. Broader monitoring programs would give a more comprehensive understanding of juvenile Steelhead Trout production and rearing and productivity contribution. Repeat sampling at consistent locations in the subbasin has enabled assessment of juvenile Steelhead Trout growth patterns. Growth rates (relative change in weight) of age-0 PITtagged parr during summer were similar across the subbasin but lower for age-1 parr in the Trout Creek watershed than the upper Wind River watershed. Yearly relative growth for parr tagged at age-0 is similar across the subbasin. Non-native Brook Trout Salvelinus confluentus are present in the subbasin, chiefly the Trout Creek watershed, and repeat sampling has allowed us to index their prevalence. Mean percent-of-catch that is Brook Trout, at four sample sites in Trout Creek, has declined from the period 1998 – 2003 to the period 2011 – 2020. Percent-of-catch and number of Brook Trout at the Trout Creek sites from 2011 through 2020 declined, though both metrics increased in 2018. Evaluation and planning of restoration efforts are critical to ensure efficient use of resources. Assessing Steelhead Trout life history variation in the Wind River subbasin will inform research and tracking of many populations and help inform habitat restoration and water allocation planning. Movement of Steelhead Trout parr from natal areas to other rearing areas raises questions regarding juvenile abundance, origin, and habitat use within watersheds. Improved PTISs and focused PIT tagging of age-0 and age-1 Steelhead Trout parr allow investigation of such questions. Increasingly detailed viable salmonid population information, such as that provided by PIT-tagging and instream PTIS networks like those in the Wind River can provide data to inform fisheries policy and management and understand life-history strategies and limiting factors. Such efforts also provide assessment of long-term effects of habitat restoration actions such as the removal of Hemlock Dam on Trout Creek, and the proposed Stage-0 restoration effort for upper Trout Creek, which would be a large-scale effort to reset sections of stream within their floodplain, restoring connectivity and interaction with surrounding landscape.

Washington↗

Essential amino acids in northern bobwhite foods

Frequent population declines, high dietary requirements for protein, and the low quality of proteins in agricultural grains led us to hypothesize that essential amino acid (EAA) deficiencies were common in northern bobwhite (Colinus virginianus) foods. We analyzed amino acid concentrations of 21 northern bobwhite foods and compared them with dietary requirements to derive values that reflected the foods' ability to meet daily bobwhite requirements of 10 EEAs. Despite adequate concentrations of crude protein, deficiencies of EAAs were detected in seeds of all species analyzed. Legumes offered the best mix of EAA, and grasses the poorest. Deficiencies of EEAs relative to maintenance requirements of adults ranged from 13% in legumes to 98% in grasses. About a third of the total nitrogen pool was composed of nonprotein nitrogen with values ranging from 25% for queensdelight stillingia (Stillingia sylvatica) to 44% for redroot amaranth (Amaranthus retroflexus). Computed biological values (ratio of EEA concentrations in seed protein to respective amounts in whole egg protein) ranged from 69 (netleaf hackberry [Celtis reticulata]) to 93 (erect dayflower [Commelina erecta] and woolly croton [Croton capitatus]). Amino acid profiles indicate forage quality better than crude protein estimates.

Journal of Wildlife Management↗

Integrated field and laboratory tests to evaluate effects of metals-impacted wetlands on amphibians: A case study from Montana

Mining activities frequently impact wildlife habitats, and a wide range of habitats may require evaluations of the linkages between wildlife and environmental stressors common to mining activities (e.g., physical alteration of habitat, releases of chemicals such as metals and other inorganic constituents as part of the mining operation). Wetlands, for example, are frequently impacted by mining activities. Within an ecological assessment for a wetland, toxicity evaluations for representative species may be advantageous to the site evaluation, since these species could be exposed to complex chemical mixtures potentially released from the site. Amphibian species common to these transition zones between terrestrial and aquatic habitats are one key biological indicator of exposure, and integrated approaches which involve both field and laboratory methods focused on amphibians are critical to the assessment process. The laboratory and field evaluations of a wetland in western Montana illustrates the integrated approach to risk assessment and causal analysis. Here, amphibians were used to evaluate the potential toxicity associated with heavy metal-laden sediments deposited in a reservoir. Field and laboratory methods were applied to a toxicity assessment for metals characteristic of mine tailings to reduce potential "lab to field" extrapolation errors and provide adaptive management programs with critical site-specific information targeted on remediation.

Conference Paper↗

Wind River Subbasin Restoration Annual Report of USGS Activities January 2021 through December 2022

We sampled juvenile wild Steelhead Trout Oncorhynchus mykiss in headwater streams of the Wind River, WA, to characterize population attributes and investigate life-history metrics, particularly migratory patterns, and early life-stage survival. We used passive integrated transponder (PIT) tagging and a series of instream PIT-tag interrogation systems (PTISs) to track juveniles and adults. The Wind River subbasin is considered a wild Steelhead refuge by Washington Department of Fish and Wildlife (WDFW). No hatchery Steelhead Trout have been released in the Wind River subbasin since 1997, and hatchery adults are estimated at less than one percent of spawners in most years. Over twenty years of Steelhead Trout status and trend monitoring and research in the subbasin is contributing to understanding of population response to numerous restoration actions in the subbasin, including removal of Hemlock Dam from Trout Creek in 2009, which had an outdated adult ladder and contributed to increased water temperatures reducing performance of juvenile Steelhead Trout. Data from our study, and companion work by Washington Department of Fish and Wildlife, are contributing to Bonneville Power Administrations (BPA) Research, Monitoring, and Evaluation (RM&E) Program Strategy of Fish Population Status Monitoring (https://www.cbfish.org/ProgramStrategy.mvc/Index). Specifically, this work addresses the sub-strategies of 1) Assessing the Status and Trends of Diversity of Natural Origin Fish Populations and Uncertainties Research regarding differing life histories of a wild Steelhead Trout population, 2) Assessing the Status and Trend of Adult Natural Origin Fish Populations, and 3) Monitoring and Evaluating the Effectiveness of Tributary Habitat Actions Relative to Environmental, Physical, or Biological Performance Objectives. During summer and fall 2021 and 2022, we PIT-tagged 1,889 and 1,391 Steelhead parr (age-0 and age-1), respectively, in the Trout Creek and upper Wind River watersheds. Age-0 parr were at lower densities in 2022 than many years due to a poor return of adult Steelhead spawners in 2022. Steelhead Trout parr were recaptured and detected through repeat headwater sampling, smolt trap operations, and instream PTISs and Columbia River PIT-tag detection infrastructure. We maintained, and upgraded in 2022, a series of six instream PTISs to monitor movement of tagged Steelhead Trout parr, smolts, and adults, providing data to population assessments, and life-cycle research and modeling. We continue to improve our PTISs in the Wind River subbasin. The improvements in siting and addition of grid power to the upper Wind River PTIS (site code WRU, rkm 27.6) during 2016 and 2017, and the addition of the Mine Reach site (site code MIN, rkm 36.0) have much improved PIT-tagged fish monitoring in the upper Wind River watershed. The paired PTIS design in the upper Wind River watershed (sites WRU and MIN) matches that in the Trout Creek watershed (sites TRC, rkm 2.0; and TC4, rkm 11.5) and will allow comparisons of Steelhead Trout population metrics between the two watersheds as response to Hemlock Dam removal continues and future restoration efforts occur in Trout Creek. During summer 2022, we upgraded three PTISs with new transceivers and new or reconfigured antennas. We replaced the Biomark 1001 Multiplexing Transceivers with Biomark MTS IS1001 Master Controller and individual IS1001 Transceivers at WRU, TRC (Trout Creek, rkm 2.0), and TC4 (Trout Creek at 43 Road Bridge, rkm 11.5). These new transceivers and antennas will improve detection performance due to increased read range and decreased susceptibility to noise. We also installed an additional IS1001 Transceiver and 11-foot antenna at WRA in summer 2021 to increase cross-channel and water column coverage. Detection data from PIT-tagged adult Steelhead Trout at PTISs allow assessment of adult escapement to tributary watersheds within the Wind River subbasin. Adult Steelhead Trout detection efficiency estimates at our primary PTIS in Trout Creek have been greater than 99 percent during six of the past eight years and have exceeded 97% at our primary PTIS in the Wind River during seven of the past eight years. Adult escapement estimates to tributary watersheds are helping us evaluate the efficacy of the 2009 removal of Hemlock Dam from rkm 2.0 of Trout Creek. The dam had potential negative effects on Steelhead Trout populations in Trout Creek due to hydrologic impairment, increased temperatures, and adult passage issues. Hemlock Dam was laddered for adult passage, but not to modern standards, which likely resulted in avoidance by some adult Steelhead Trout. Detections at the instream PTISs have demonstrated trends of age-0 and age-1 parr emigration from natal areas during summer and fall, in addition to the expected movement of parr and smolts in spring. We have estimated that from 15 to 51% of parr tagged as age-0 fish in headwater areas make downstream migrations at age 1 for additional rearing. Downstream movement occurs primarily during spring but also in fall. We have estimated that up to 27% of Steelhead Trout parr, tagged as age-1 fish, make downstream migrations during fall. Fall migration of age-1 parr has been more common in the upper Wind River watershed than the Trout Creek watershed. These findings raise questions about where parr most successfully rear and whether migrations are density- or habitat-quality driven. Broader monitoring programs would give a more comprehensive understanding of juvenile Steelhead Trout production and rearing and contributions to adult recruitment from varied rearing strategies. Repeat sampling at consistent locations in the subbasin has enabled assessment of juvenile Steelhead Trout growth patterns. Growth rates (relative change in weight) of age-0 PIT-tagged parr during summer were similar across the subbasin, though slightly lower in the Trout Creek watershed. The greatest summer growth rate was in the mainstem of the Wind River (rkm 37 and 41). Summer growth rates were lower for age-1 parr in the Trout Creek watershed than the upper Wind River watershed. Yearly relative growth was similar across the subbasin for both age-0 and age-1 tagged parr. Lower Layout Creek had the highest yearly growth rate of parr from age-0 to age-1. Mainstem Wind River (rkm 37) had the highest yearly growth rate of parr from age-1 to age-2. Non-native Brook Trout Salvelinus fontinalis are present in the subbasin, chiefly the Trout Creek watershed, and repeat sampling provides an index of their prevalence. Mean percent-of-catch that is Brook Trout, at four sample sites in Trout Creek, has declined from the period 1998 2003 to the period 2011 2022. Percent-of-catch and number of Brook Trout at the Trout Creek sites from 2011 through 2022 has generally declined, though both metrics have been somewhat variable. Evaluation and planning of habitat restoration efforts are critical to ensure efficient use of money and resources. Assessing Steelhead Trout life history variation in the Wind River subbasin will inform research and tracking of many populations and help inform habitat restoration and water allocation planning. Movement of Steelhead Trout parr from natal areas to other rearing areas raises questions regarding juvenile abundance, origin, and habitat use within watersheds. Improved PTISs and focused PIT-tagging of age-0 and age-1 Steelhead Trout parr allow investigation of such questions. Increasingly detailed viable salmonid population information, such as that provided by PIT-tagging and instream PTIS networks like those in the Wind River, can provide data to inform fisheries policy and management and understand life-history strategies and limiting factors. Such efforts also enable assessment of long-term effects of habitat restoration actions such as the removal of Hemlock Dam on Trout Creek, and the proposed Stage-0 restoration effort for upper Trout Creek, which would be a large-scale effort to reset sections of stream within their floodplain, restoring connectivity and interaction with surrounding landscape.

Report↗

Accounting for phenology in the analysis of animal movement

The analysis of animal tracking data provides important scientific understanding and discovery in ecology. Observations of animal trajectories using telemetry devices provide researchers with information about the way animals interact with their environment and each other. For many species, specific geographical features in the landscape can have a strong effect on behavior. Such features may correspond to a single point (eg, dens or kill sites), or to higher dimensional subspaces (eg, rivers or lakes). Features may be relatively static in time (eg, coastlines or home‐range centers), or may be dynamic (eg, sea ice extent or areas of high‐quality forage for herbivores). We introduce a novel model for animal movement that incorporates active selection for dynamic features in a landscape. Our approach is motivated by the study of polar bear (Ursus maritimus) movement. During the sea ice melt season, polar bears spend much of their time on sea ice above shallow, biologically productive water where they hunt seals. The changing distribution and characteristics of sea ice throughout the year mean that the location of valuable habitat is constantly shifting. We develop a model for the movement of polar bears that accounts for the effect of this important landscape feature. We introduce a two‐stage procedure for approximate Bayesian inference that allows us to analyze over 300 000 observed locations of 186 polar bears from 2012 to 2016. We use our model to estimate a spatial boundary of interest to wildlife managers that separates two subpopulations of polar bears from the Beaufort and Chukchi seas.

Biometrics↗

Interim report on the scientific investigations in the Animas River watershed, Colorado to facilitate remediation decisions by the U.S. Bureau of Land Management and the U.S. Forest Service, March 29, 2000 meeting, Denver, Colo.

INTRODUCTION The joint U.S. Department of the Interior and U.S. Department of Agriculture Abandoned Mine Lands Initiative (AMLI) was developed as a collaborative effort between the Federal land management agencies (FLMA, that is the U.S. Bureau of Land Management and the U.S. Forest Service) and the U.S. Geological Survey (USGS) in 1996. The stated goal of the AML Initiative was to develop a strategy for gathering and communicating the scientific information needed to develop effective and cost-efficient remediation of abandoned mines within the framework of a watershed. Four primary objectives of the AMLI are to: 1. Provide the scientific information needed (in the short-term) by the FLMAs to make decisions related to the design and implementation of cleanup actions, 2. Develop a multi-disciplined, multi-division approach that integrates geologic, hydrologic, geochemical and ecological information into a knowledge base for sound decision making, 3. Transfer technologies developed within the scientific programs of the USGS to the field and demonstrate their suitability to solve real, practical problems, and 4. Establish working relationships among involved members of land management and regulatory agencies within the framework of a watershed approach to the cleanup of abandoned mines. Long-term process-based research, including development of analytical tools, is recognized as being critical to the long-term success in remediating watersheds impacted by historical mining activities (AML 5-year plan, http://amli.usgs.gov/amli). In a meeting of Federal agencies (U.S. Bureau of Land Management [BLM], U.S. Bureau of Reclamation [BOR], U.S. National Park Service [NPS], U.S. Forest Service [USFS], the U.S. Environmental Protection Agency [EPA], the U.S. Fish and Wildlife Service [F&WS]), and State agencies (Colorado Division of Public Health and Environment, Colorado Division of Mines and Geology), several watersheds were examined within the state whose water quality was presumed to be impacted by historical mining activities. The Animas River watershed (fig. 1) was selected by the State and Federal agencies as one of two watersheds in the U.S. to be studied in detail by the USGS in the AML Initiative. Beginning in October 1997, each of the four Divisions of the USGS (Water Resources, Geologic, Biological Resources, and National Mapping) initiated a collaborative integrated science study of the watershed. Funds were provided from USGS base funding to each of the four Divisions in response to the priorities set by Congressional action and within the flexibility provided by the budgetary framework funding individual research programs. The AML Initiative provides for a five-year focused scientific effort in the two watersheds with final synthesis of the scientific results from each to be published in 2001. Publications are released on the AML web site on a regular basis (http://amli.usgs.gov/amli). On March 29, 2000, the USGS hosted a meeting for the BLM and USFS to discuss remediation options that were under consideration for the summer of 2000. The purpose of this report is to provide an overview of the scientific rational provided by the USGS to meet objective one above, and to summarize our preliminary interpretations of our data. Additional information from sites on private lands have been collected by the State of Colorado, EPA, and the ARSG. Unfortunately, these data have not been fully supplied to the USGS so our conclusions are based only upon our data. These interpretations provide science-based constraints on possible remediation options to be considered by the FLMA, the State, and local property owners in the Animas River watershed. The report is presented in outline format to facilitate discussion of remediation options at the March 29, 2000 meeting. Not all historical mining sites within the watershed are on public lands. This should not be construed to be a final report of the USGS

Denver↗

Modeling occupancy of rare stream fish species in the upper Cumberland and Kentucky River Basins

Biological conservation often requires an understanding of how environmental conditions affect species occurrence and detection probabilities. We used a hierarchical framework to evaluate these effects for several Appalachian stream fish species of conservation concern: Chrosomus cumberlandensis (BSD; blackside dace), Etheostoma sagitta (CAD; Cumberland arrow darter), and Etheostoma spilotum (KAD; Kentucky arrow darter). Etheostoma susanae (Cumberland darter) also is present in the study area but was too rare to model in this analysis. In this study, conducted by the U.S. Geological Survey in cooperation with the U.S. Fish and Wildlife Service, fish and habitat data were collected from 205 randomly selected stream sites in the upper Cumberland and Kentucky River Basins (120 and 85 sites, respectively) of Kentucky and Tennessee. Sites were sampled with 10 spatial replicates (2 meter x 5 meter electrofishing zones) to enable estimation of detection probabilities and environmental effects. The best models (that is, lowest Akaike information criterion scores) showed the effects of agriculture (negative) on occurrence of BSD and stream conductivity (negative) on occurrence of CAD and KAD. These effects were statistically more important than measures of basin area, elevation, and substrate size. Conductivity and agriculture showed nonlinear effects on species occurrence, and effects of conductivity were more precise above 400 microsiemens per centimeter than below this threshold. Models incorporated detection-level effects of electrofishing time (positive), flow velocity (negative), sand substrate (positive), and gravel/cobble substrate (negative). Models accounting for detection of BSD estimated occupancy rates similar to the observed proportion of occupied sites (0.10), but the best-supported models for CAD and KAD increased expected occupancy by about 4 percent for each species (from 0.17 to 0.21 for CAD and from 0.07 to 0.11 for KAD). Results of this study provide new inferences for modeling stream fish occurrence and detection processes and highlight the importance of continued monitoring and assessment of rare fish species in Appalachian headwater streams.

Kentucky, Tennessee, Virginia↗

Wind River subbasin restoration: Annual report of US..Geological Survey activities, January 2018 through December 2018

We sampled juvenile wild Steelhead Oncorhynchus mykiss in headwater streams of the Wind River, WA, to characterize populations and investigate life-history metrics, particularly migratory patterns. We used Passive Integrated Transponder (PIT)-tagging and a series of instream PIT-tag interrogation systems (PTISs) to track juveniles. The Wind River subbasin is considered a wild Steelhead refuge by Washington Department of Fish and Wildlife (WDFW). No hatchery Steelhead have been planted in the Wind River subbasin since 1997, and hatchery adults are estimated to be less than one percent of spawners in most years (pers comm. Thomas Buehrens, Washington Department of Fish and Wildlife). Our repeated headwater sampling of consistent sites in the Wind River subbasin has also allowed us to track relative abundance of Brook Trout, a non-native species to the Wind River. Our work is contributing to understanding of Steelhead population response to numerous restoration actions in the subbasin, including removal of Hemlock Dam from Trout Creek in 2009, where our PTISs are helping to quantify adult response. Data from our study, and companion work by Washington Department of Fish and Wildlife, are contributing to Bonneville Power Administration’s (BPA) Research Monitoring and Evaluation (RM&E) Program Strategy of Fish Population Status Monitoring (www.cbfish.org/ProgramStrategy.mvc/ViewProgramStrategySummary/1). Specifically this work addresses the sub-strategies of: 1) Assessing the Status and Trends of Diversity of Natural Origin Fish Populations and to Uncertainties Research regarding differing life histories of a wild Steelhead population, 2) Assessing the Status and Trend of Adult Natural Origin Fish Populations, and 3) Monitoring and Evaluating the Effectiveness of Tributary Habitat Actions Relative to Environmental, Physical, or Biological Performance Objectives. Our headwaters parr PIT tagging, WDFW parr, smolt, and adult tagging and our instream PTISs are providing data on movements and life histories of parr, smolt, and adult Steelhead. During summer 2018, we PIT-tagged 1,592 age-0 and age-1 Steelhead parr in headwater areas of the Wind River subbasin to characterize population traits and investigate life-history diversity, including growth and pre-smolt downstream movement. Repeat headwater sampling and smolt trap operations provide opportunities for recapture, and instream PTISs and Columbia River infrastructure provide opportunity for detection of PIT-tagged fish. Throughout the year, we maintained a series of six instream PTISs to monitor movement of tagged Steelhead parr, smolts, and adults. Detections at the instream PTISs have demonstrated trends of age-0 and age-1 parr emigration from natal areas during summer and fall, in addition to the expected movement of parr and smolts in spring. Substantial numbers of parr make downstream movements as age-1 fish. We have estimated that from 15 to 33 percent of parr tagged as age-0 fish make downstream migrations at age-1 for additional rearing. We have estimated that from 1 to 27 percent of parr tagged as age-1 fish make downstream migrations during fall. These findings raise many questions about parr rearing strategies, habitat use, and success of these migrants and suggest a need for broader monitoring of juvenile Steelhead in some river systems to fully document juvenile production. Long-term monitoring of PIT-tagged fish is providing information on contribution of various life-history strategies to smolt production and adult returns. Movements of PIT-tagged adult Steelhead were recorded at instream PTISs. These data have allowed assessment of adult returns to tributary watersheds within the Wind River subbasin. Detection efficiency of adult PIT-tagged Steelhead at our primary adult-monitoring PTIS in Trout Creek has been greater than 92 percent during 6 of the past 7 years. This is providing excellent data to estimate adult returns to this watershed. Determination of adult use of tributary watersheds is providing data to help evaluate the efficacy of the removal of Hemlock Dam on Trout Creek. Hemlock Dam, located at rkm 2.0 of Trout Creek, was removed in summer 2009. The dam contributed to hydrologic impairment of Trout Creek and had potential negative effects on Steelhead. The improvements made to the upper Wind River PTIS (site code WRU at rkm 28.3; better site characteristics and grid power) during 2016 and 2017, and a planned new site in the Mine Reach of the upper Wind River, will allow estimates of subbasin adult escapement like those in Trout Creek. During 2018, we also completed planning and permitting with U.S. Forest Service for a new PTIS site at rkm 36 of the Wind River (the Mine Reach, mentioned above). This site will replace two sites (one in Paradise Creek and one at rkm 41 of the Wind River), which had operational challenges due to lack of adequate solar power and winter difficulties. The new Mine Reach PTIS site at rkm 36, will have better solar exposure, fewer winter operations difficulties, and provide opportunity to detect fish from juvenile sampling sites that were downstream of the previous two PTISs. The more consistent operation of the new Mine Reach PTIS site will increase our ability to estimate migrant abundance as all the juveniles tagged upstream of it will be subject to the same potential detection history, instead of three different potential detection histories as before. Additionally, with the new Mine Reach PTIS site lower in the watershed, it will subject more PIT-tagged adult Steelhead to detection and provide ability to generate a nonbiased adult-detection efficiency estimate for the WRU PTIS at rkm 28.3 of the Wind River. This will provide the opportunity to estimate yearly adult Steelhead abundance to the upper Wind watershed area. Permitting is complete and some supplies have been purchased to build and install this new site in 2019. Repeat sampling at consistent locations in the subbasin has allowed investigation into juvenile Steelhead growth patterns. Growth rates (relative change in weight) of age-0 PIT-tagged parr during summer are similar across the subbasin, but lower for age-1 parr in the Trout Creek watershed than the upper Wind River watershed. Yearly growth for parr tagged at age-0 is similar across the subbasin. Yearly growth for parr tagged at age-1 is lowest in Martha Creek, but similar elsewhere. Non-native Brook Trout are present in portions of the subbasin, chiefly the Trout Creek watershed, and repeat sampling has allowed us to index their prevalence. Percentage of catch that is Brook Trout at each of four sample sites in Trout Creek have declined from the period 1998 – 2003 to the period 2011 – 2018. There was a pattern of decline in percent of catch and number of Brook Trout at the Trout Creek sites from 2011 through 2016, though a slight upward trend during 2017 and 2018 has been evident. Evaluating and planning restoration efforts are of interest to many managers and agencies to ensure efficient use of resources. The evaluation of various life-histories of Steelhead within the Wind River subbasin will provide information to better track populations, and to direct habitat restoration and water allocation planning. Movement of Steelhead parr raises many questions regarding estimating juvenile abundance, origin, and habitat use within watersheds. Improved PTISs and focused PIT tagging of age-0 and age-1 Steelhead parr are increasingly allowing us to investigate such questions. Increasingly detailed Viable Salmonid Population information, such as that provided by PIT-tagging and instream PTISs networks like those in the Wind River subbasin, provide data to inform policy and management, as life-history strategies and production bottlenecks are identified and understood.

Washington↗

Social identity, values, and trust in government: How stakeholder group, ideology, and wildlife value orientations relate to trust in a state agency for wildlife management

Our objective was to understand how social identity and values influenced general public and stakeholder trust in a state wildlife management agency (SWMA). In particular, we wanted to examine how stakeholder group, political ideology, and wildlife value orientation influenced trust in a SWMA. Data were derived from a study of state residents, hunters and livestock producers related to wolf management. Results suggest differences in agency trust correlated with all three factors and the interaction between stakeholder group and wildlife value orientation. Livestock producers reported lower levels of trust in the agency, compared to hunters and the general public. Individuals with conservative or middle-of-the-road ideologies reported less trust in the agency than individuals with a liberal ideology did. Respondents with traditional wildlife values also had less trust in the agency compared to mutualist, pluralist, and distanced respondents. Results suggested similarities in the ideologies and wildlife value orientations of livestock producers and hunters, although these groups differed significantly in their trust in the agency. Agencies face the challenge of managing common-pool, public-trust resources for stakeholders who hold different values and ideologies, and who are differentially affected by management decisions.

Minnesota↗

Blood serum chemistry of wild Alaskan Black-capped Chickadees ( Poecile atricapillus ) with avian keratin disorder

We measured serum chemistries in wild Black-capped Chickadees ( Poecile atricapillus ) from Alaska to test for potential differences associated with beak deformities characteristic of avian keratin disorder. Lower uric acid in affected birds was the only difference detected between groups, although sample sizes were small. This difference could be associated with fasting or malnutrition in birds with beak deformities, but it is challenging to interpret its biologic significance without reference values. Black-capped Chickadees had high levels of aspartate aminotransferase, lactate dehydrogenase, and creatine kinase relative to reference values for companion birds. However, all serum chemistry parameters from our study were within the range of values reported from other apparently healthy wild-caught birds.

Journal of Wildlife Diseases↗

Gonadal abnormalities in frogs (Lithobates spp.) collected from managed wetlands in an agricultural region of Nebraska, USA

Nebraska's Rainwater Basin (RWB) provides important wetland habitat for North American migratory birds. Concern exists that pesticide and nutrient runoff from surrounding row-crops enters wetlands degrading water quality and adversely affecting birds and wildlife. Frogs may be especially vulnerable. Plains leopard (Lithobates blairi) metamorphs from RWB wetlands with varying concentrations of pesticides were evaluated for a suite of biomarkers of exposure to endocrine active chemicals. Froglets had ovarian dysgenesis, high rates of testicular oocytes, and female-biased sex ratios however, there was no clear statistical association between pesticide concentrations and biomarkers. Data interpretation was hindered because timing and duration of exposures were unknown and due to an incomplete understanding of L. blairi sexual development. Emphasis is on describing the complex developmental biology of closely-related leopard frogs, how this understanding can explain RWB L. blairi anomalies, and the need for sampling at the appropriate life stage.

Nebraska↗