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Problems of stock definition in estimating relative contributions of Atlantic striped bass to the coastal fishery

Stock contribution studies of mixed‐stock fisheries rely on the application of classification algorithms to samples of unknown origin. Although the performance of these algorithms can be assessed, there are no guidelines regarding decisions about including minor stocks, pooling stocks into regional groups, or sampling discrete substocks to adequately characterize a stock. We examined these questions for striped bass Morone saxatilis of the U.S. Atlantic coast by applying linear discriminant functions to meristic and morphometric data from fish collected from spawning areas. Some of our samples were from the Hudson and Roanoke rivers and four tributaries of the Chesapeake Bay. We also collected fish of mixed‐stock origin from the Atlantic Ocean near Montauk, New York. Inclusion of the minor stock from the Roanoke River in the classification algorithm decreased the correct‐classification rate, whereas grouping of the Roanoke River and Chesapeake Bay stock into a regional (“southern”) group increased the overall resolution. The increased resolution was offset by our inability to obtain separate contribution estimates of the groups that were pooled. Although multivariate analysis of variance indicated significant differences among Chesapeake Bay substocks, increasing the number of substocks in the discriminant analysis decreased the overall correct‐classification rate, Although the inclusion of one, two, three, or four substocks in the classification algorithm did not greatly affect the overall correct‐classification rates, the specific combination of substocks significantly affected the relative contribution estimates derived from the mixed‐stock sample, Future studies of this kind must balance the costs and benefits of including minor stocks and would profit from examination of the variation in discriminant characters among all Chesapeake Bay substocks.

Maryland↗

Effects of multiple acute stressors on the predator avoidance ability and physiology of juvenile Chinook salmon

Northern squaw fish Ptychocheilus oregonensis are the predominant predators of juvenile Pacific salmonids Oncorhynchus spp. in the Columbia River, and their predation rates are greatest just below dams. Because juvenile salmonids are commonly subjected to multiple stressors at dams in the course of their seaward migration, high predation rates below dams may be due in part to an increase in the vulnerability of stressed fish. I conducted laboratory experiments to examine the predator avoidance ability and physiological stress responses of juvenile chinook salmon O. tshawytscha subjected to treatments (stressors) designed to simulate routine hatchery practices (multiple handlings) or dam passage (multiple agitations). Both stressors resulted in lethargic behavior in the fish, and agitation also caused disorieniation and occasional injury. When equal numbers of stressed and unstressed fish were exposed to northern squawfish for up to 1 h, significantly more stressed fish were eaten, but this effect was not evident during longer exposures. The lack of differential predation in trials lasting up to 24 h can be explained by the rapid development of schooling behavior in the prey, but other possibilities exist, such as changing ratios of stressed and unstressed prey over time. Concentrations of plasma cortisol, glucose, and lactate in fish subjected to multiple stressors were similar and sometimes cumulative, returned to prestress levels within 6-24 h, and correlated poorly with predator avoidance ability. My results suggest that juvenile salmonids are capable of avoiding predators within 1 h after being subjected to multiple acute stressors even though physiological homeostasis may be altered for up to 24 h. Therefore, because juvenile salmonids typically reside in lailrace areas for only a short time after dam passage, measures aimed at reducing physical stress or protecting them as they migrate through dam tailraces may help alleviate the relatively intense predation in these areas.

Transactions of the American Fisheries Society↗

Effects of salinity on striped bass eggs and larvae from the Savannah River, Georgia

Operation of a tide gate installed in the Savannah River by the U.S. Army Corps of Engineers to reduce dredging activities increased salinities upstream in important spawning habitat for striped bass Morone saxatilis . To assess the effects of salinity on survival and growth of striped bass at early life stages, newly fertilized eggs and 48‐h‐posthatch larvae were exposed to serial dilutions of seawater, with salinities ranging from 0 to 33‰ (g/L) in increments of 3‰; in addition, older larvae (5‐d posthatch) were exposed to salinities of 0, 6, 12, 18, and 24‰. Eggs were exposed until 24 h posthatch, 48‐h‐posthatch larvae were exposed for 10 d, and 5‐d‐posthatch larvae were exposed for 6 d. Eggs died within 24 h in salinities greater than 18‰. Both survival and total length of larvae hatched from eggs exposed to salinities of 15‰ or higher were reduced. Percent mortality and mean total lengths of newly hatched larvae followed the same pattern for each of three sets of salinity regimes (i.e., changes in salinities over time) that striped bass eggs might encounter during passage downstream in the Savannah River. Hardening eggs in freshwater did not increase survival or length of hatched larvae over that shown by eggs hardened in saline water. The 5‐d‐posthatch larvae were less sensitive to salinity than the 48‐h‐posthatch larvae. Survival of larvae was negatively correlated with both salinity and exposure time. For 48‐h‐posthatch larvae, the 10‐d LC50 (the salinity lethal to 50% of the test fish within 10 d) was 10‰. Probabilities of survival for larval striped bass exposed to different salinities for different amounts of time can be estimated from curves generated from models of survival analysis. Salinities judged to be critical to Savannah River striped bass eggs and larvae are those greater than 9‰.

Georgia↗

Importance of spatial pattern in estimating predation on juvenile salmonids in the Columbia River

The impact of piscivores in aquatic systems is often estimated by assuming that predation rate and predator density can be characterized as means throughout large, homogeneous areas. Predation losses of juvenile Pacific salmonids Oncorhynchus spp. migrating through Columbia River reservoirs were previously estimated with the assumption that each reservoir consisted of one or two homogeneous areas. Data from the John Day Reservoir and throughout the river system showed that predation rate and predator density vary greatly between near-dam and midreservoir areas, suggesting that reservoirs in the Columbia River should be divided into at least three or four areas for estimating salmonid losses. For example, the estimated number of salmonids annually eaten by northern squawfish Ptychocheilus oregonensis in John Day Reservoir decreased from 2.9 million when all samples in the reservoir were pooled into one area, to 1.4 million when samples were partitioned among four areas. Variance about the estimates also decreased steadily with finer partitioning. Mortality of juvenile salmon from predation was substantial with any type of partitioning; however, spatial variation in predation rates and other density-dependent processes may be especially important in river models of migrating juvenile salmon that repeatedly apply predation rates in a series of reservoirs or river reaches.

Transactions of the American Fisheries Society↗

Tag recovery estimates of migration of striped bass from spawning areas of the Chesapeake Bay

In 1988–1991 striped bass Morone saxatilis were collected for tagging from various spawning areas within the Hudson River (New York) and the Chesapeake Bay (Maryland). The fish were tagged and released during traditional periods of spawning and recovered by commercial and recreational fishermen. The proportion of fish that migrated in spring–fall from spawning areas in Chesapeake Bay to more northern waters of mid‐Atlantic and New England states was estimated from the geographically stratified tag returns. Most of the tagged fish were 40–100 cm total length (TL). The estimated proportion of migrant striped bass increased with body size, and nearly all fish larger than 100 cm TL left the bay during the spring–fall migration. Sex‐specific differences in migration appear to be associated with the differences in body size of mature males and females, thus lending support to previously hypothesized patterns of striped bass migration.

Maryland↗

Pattern of shoreline spawning by sockeye salmon in a glacially turbid lake: Evidence for subpopulation differentiation

Alaskan sockeye salmon Oncorhynchus nerka typically spawn in lake tributaries during summer (early run) and along clear‐water lake shorelines and outlet rivers during fall (late run). Production at the glacially turbid Tustumena Lake and its outlet, the Kasilof River (south‐central Alaska), was thought to be limited to a single run of sockeye salmon that spawned in the lakeˈs clear‐water tributaries. However. up to 40% of the returning sockeye salmon enumerated by sonar as they entered the lake could not be accounted for during lake tributary surveys, which suggested either substantial counting errors or that a large number of fish spawned in the lake itself. Lake shoreline spawning had not been documented in a glacially turbid system. We determined the distribution and pattern of sockeye salmon spawning in the Tustumena Lake system from 1989 to 1991 based on fish collected and radiotagged in the Kasilof River. Spawning areas and times were determined for 324 of 413 sockeye salmon tracked upstream into the lake after release. Of these, 224 fish spawned in tributaries by mid‐August and 100 spawned along shoreline areas of the lake during late August. In an additional effort, a distinct late run was discovered that spawned in the Kasilof River at the end of September. Between tributary and shoreline spawners, run and spawning time distributions were significantly different. The number of shoreline spawners was relatively stable and independent of annual escapement levels during the study, which suggests that the shoreline spawning component is distinct and not surplus production from an undifferentiated run. Since Tustumena Lake has been fully deglaciated for only about 2,000 years and is still significantly influenced by glacier meltwater, this diversification of spawning populations is probably a relatively recent and ongoing event.

Alaska↗

Evidence for lack of homing by sea lampreys

Recently metamorphosed sea lampreys Petromyzon marinus were captured in the Devil River, a tributary to Lake Huron, during summer and autumn 1990. They were tagged with a coded wire tag and returned to the river to continue their migration to Lake Huron to begin the parasitic (juvenile) phase of their life. During the spawning run in spring 1992 when the tagged animals were expected to mature and return to spawn, sea lampreys were trapped in nine tributaries to Lake Huron, including the Devil River; 47,946 animals were examined for coded wire tags, and 41 tagged animals were recovered. None of the 45 mature sea lampreys captured in the Devil River in 1992 were tagged, a proportion (0%) significantly lower than the proportion of the recently metamorphosed sea lampreys tagged in 1990. The distribution of tag recoveries among streams lakewide, however, was proportional to catch. Tagged sea lampreys did not appear to home, but instead seemed to select spawning streams through innate attraction to other sensory cues.

Transactions of the American Fisheries Society↗

Some tests of the "migration hypothesis" for anadromous Dolly Varden (southern form)

Some aspects of a previously described migratory paradigm for the southern form of anadromous Dolly Varden Salvelinus malma were investigated with seven 3‐year mark–recapture experiments on fish that used lakes in eight watersheds as their winter residence. Weirs on Kodiak Island, around Prince William Sound, and near Juneau, Alaska, were used to capture Dolly Varden as they emigrated to the sea each spring. Dolly Varden (≥200 mm fork length) were individually marked during the first year of each experiment (1989 or 1990), and log‐linear models of their capture histories were used to estimate probabilities of capture during the second year (1990 or 1991). Our observations on timing of spring emigration and dispersal of Dolly Varden at sea confirm observations from earlier studies. Our results support the paradigm that Dolly Varden home to the same lacustrine watershed when overwintering in fresh water, as more than 98% of the recaptured fish did so. Our results contradicted the paradigm that Dolly Varden return to lakes each fall, for across study populations, 14–58% failed to return. The most probable explanation for this anomalous behavior is that some Dolly Varden spend the winter at sea. Differences in maturity, size, and growth of Dolly Varden and timing of their entrance into salt water during spring emigration were excluded as causes of this anomalous behavior.

Transactions of the American Fisheries Society↗

Diet and feeding periodicity of ruffe in the St. Louis River estuary, Lake Superior

Ruffe Gymnocephalus cernuus , a percid native to Europe and Asia, is established in the Lake Superior drainage and could have negative impacts on native fish through competition for forage and predation on fish eggs. We investigated the diet of ruffes in the 4,654-ha St. Louis River estuary in May–October 1989–1990 and the feeding periodicity of ruffes in two adjacent habitats during five 24-h periods in summers 1990–1991. Ruffes were primarily benthophagous. Age-0 ruffes fed mostly on cladocerans and copepods in early summer and midge larvae (Chironomidae) in late summer and fall. Adult ruffes less than 12 cm fed mostly on midges and other macrobenthos but also consumed large numbers of microcrustaceans. Adult ruffes 12 cm and larger fed mostly on midges, burrowing mayflies Hexagenia spp., and caddisflies (Trichoptera). Ruffes consumed few fish eggs. Adult ruffes in deeper waters and all age-0 ruffes fed throughout the day as indicated by weight patterns of stomach contents. However, adult ruffes generally moved to shallower waters at night to feed most heavily. Results of this study indicate that ruffes will probably compete with other benthic-feeding fishes such as yellow perch Perca flavescens and trout-perch Percopsis omiscomaycus .

Transactions of the American Fisheries Society↗

Growth and survival of newly parasitic sea lampreys at representative winter temperatures

Larval sea lampreys Petromyzon marinus begin to metamorphose into their parasitic phase in July and migrate to the Great Lakes either in autumn, when they immediately feed on fish, or in spring after overwintering in the stream substrate. Survival and growth of newly parasitic autumn migrants (mean weight, 4.18 g) differed significantly between temperature treatments when sea lampreys were held over winter and allowed to feed on longnose suckers Catostomus catostomus at either the maximum available temperature (&tilde;4&deg;C) or normal surface temperature (minimum <1&deg;C) in the Great Lakes during winter. Survival from December 1990 to June 1991 was 60% for the animals held in the warmer water but only 30% for the animals held in the colder water until 23 April. The average increase in weight was 8.23 g for the 35 survivors in the warmer water but only 5.15 g for the 17 survivors in the colder water. Average increases in weight from December to May for sea lampreys at both temperatures were 3.8 to 6.6 times greater than increases reported previously. A newly metamorphosed sea lamprey that migrates to the Great Lakes in autumn could be 2.5 to 3 times larger in June than one that overwinters in the stream substrate, where it cannot feed, and migrates in spring. Hence, autumn migrants may have an advantage in growth and survival over spring migrants, particularly if food supply is adequate in the warmest stratum of the lake during winter.

Transactions of the American Fisheries Society↗

Evidence of impaired reproduction by Western mosquitofish inhabiting seleniferous agricultural drainwater

Western mosquitofish Gambusia affinis from the San Luis Drain (SLD), a major conveyance system for seleniferous agricultural subsurface (tile) drainage water in the San Joaquin Valley, California, experienced lower reproductive success than did mosquitofish from a nearby reference area (Pond 26 at the Volta Wildlife Area, VOLTA) that had no history of receiving tile drainage. At birth, mean survival of SLD fry was 70% in 1984 and 77% in 1985, whereas mean survival of VOLTA fry was greater than 99% in 1984 and 97% in 1985. Measurements of total length (TL) of offspring born to SLD females in 1985 showed that stillborn fry (mean TL, 7.5 mm) were significantly shorter than live fry (mean TL, 9.4 mm). In addition, most stillborn fry were at earlier stages of development (contained prominent yolk sacs or occasionally resembled eyed embryos) than most live fry (yolk sacs were fully resorbed). In comparison, stillborn and live fry born to VOLTA females did not differ significantly in TL (mean for stillborn fry, 7.1 mm; mean for live fry, 7.5 mm), and nearly all fry had resorbed their yolk sacs. Mosquitofish from the SLD contained over 100 μg/g (whole‐body concentration in dry weight) of selenium, whereas those from VOLTA averaged 1.5 μg/g. Although experiments are still needed to establish cause and effect, the high incidence of abnormal births of mosquitofish from the SLD is seemingly a response to selenium toxicity.

California↗

Ebb and flow of encroachment by nonnative rainbow trout in a small stream in the southern Appalachian Mountains

Brook trout Salvelinus fontinalis is the native salmonid species of streams in the southern Appalachian Mountains. The present distribution of this species, once widespread from headwaters to lower reaches of large streams, is restricted to mostly headwater areas. Changes in the distribution of native brook trout in the presence ofˈ nonnative rainbow trout Oncorhynchus mykiss have been documented in Great Smoky Mountains National Park. When rainbow trout were first found in a tributary (Rock Creek) in the park in 1979, a study was begun to assess changes through time in distribution and abundance of rainbow trout in Rock Creek and to compare the brook trout and rainbow trout associations in Rock Creek with associations found in other park streams. Abundance of brook trout was low in the downstream sections of Rock Creek in 1979–1993. Brook trout abundance was highest in the steep‐gradient, pool‐dominated headwater section which was only 2 km from the confluence of Rock Creek and Cosby Creek. Rainbow trout were present in low densities in Rock Creek during the same period. Although rainbow trout were most abundant in the lower stream sections and never found in the headwater section, adult and age‐0 rainbow trout were found in the middle section in 1988. Rainbow trout were absent in the middle section in 1991, but one large adult rainbow trout was present in the section in 1992 and 1993. Floods, freshets, and periods of low stream discharge appeared to play an important role in the distribution and population structure of rainbow trout in Rock Creek. The lower portion of Rock Creek was poor trout habitat because the sections were dominated by cobble–rubble substrate and shallow riffle areas. Stream habitat appeared to be better suited for brook trout than for rainbow trout in the steep‐gradient upstream sections which were dominated by boulder‐cobble substrate and deep pools. The results of this study suggest that encroachment by rainbow trout can exhibit considerable ebb and flow in steep‐gradient tributaries in the park, and they suggest substantial evolutionary adaptation by brook trout to the hydrological conditions in the Rock Creek drainage.

North Carolina, Tennessee↗

A non-lethal measure of smolt status of juvenile steelhead based on body morphology

A nonlethal morphometric method to assess smolt status of juvenile steelhead Oncorhynchus mykiss was validated. Fish were collected from hatcheries before release and during their seaward migration in a large river system. Fifteen anatomical landmarks were digitized from photographs of each fish, resulting in 34 morphometric characters based on a truss network. Principal component and canonical discriminant function analyses were used to assess smolt status. A single canonical variate was significantly correlated with gill Na + ,K + ‐ATPase activity, a commonly used measure of smolt status. This nonlethal method may be a useful measure of smolt status when sacrificing fish is not desirable or possible; it requires little training to perform, but it does require a larger sample size than some other methods.

Transactions of the American Fisheries Society↗

Developmental instability in the Pacific Hake parasitized by the Myxosporean Kudoa spp.

A morphometric study of the otoliths of a population of Pacific hake Merluccius productus was conducted to detect developmental instability (DI) related to parasitism by Myxosporeans, Kudoa spp. We report a link between parasitism and DI, which we measured as fluctuating asymmetry by comparing the right–left differences in otolith shape, width, and weight in the same fish. Otolith shape provided the most sensitive measurement of DI asymmetry. Results suggest that Kudoa spp. could be a strong agent of selection in the Pacific hake.

Transactions of the American Fisheries Society↗

Size-dependent effects of continuous and intermittent feeding on starvation time and mass loss in starving yellow perch larvae and juveniles

Starvation rates of fish larvae living in patchy prey environments can have an important impact on cohort survival and recruitment. Despite this, little is known about how fluctuations in feeding experience influence starvation resistance and how this changes with ontogeny. Fish previously exposed to fluctuating food densities may not respond to long periods without food in the same way as fish previously exposed to a constant prey density. In a series of laboratory experiments with larvae and juveniles of yellow perch Perca flavescens , we tested the effects of continuous and intermittent feeding on times to starvation and on mass loss up to death from starvation for fish with initial total lengths of 10, 15, and 20 mm. Results indicated that proportional mass loss up to starvation was independent of fish mass, but that it did depend on feeding history. Fish that fed continuously before starvation all died after losing the same proportion of body mass (55%), but intermittent feeders died when they were slightly heavier (51–46% of body mass lost). Times to 50% mortality followed a different pattern; there was no significant difference in times to 50% mortality for fish that had fed continuously or intermittently for the same number of days before starvation. We conclude that short‐term fluctuations (≤4 d) in food availability do not appear to affect times to starvation but do influence mass loss during starvation in young yellow perch.

Transactions of the American Fisheries Society↗

Spawning of the shortnose sturgeon the Merrimack River, Massachusetts

We tracked 10 ultrasonically tagged shortnose sturgeons Acipenser brevirostrurn during spring in the Merrimack River to investigate spawning. Seven fish in 1989 and six fish in 1990 were tracked intensively to identify the timing and location of spawning and to characterize spawning habitat. In mid‐April 1989 and 1990, fish moved upstream to just below head of tide, concentrating in a 2‐km reach at river kilometers 30–32 (measured from the mouth) at Haverhill, Massachusetts. The estimated spawning time was a 5‐d period (26–30 April) in 1989 and an 8‐d period (22–29 April) in 1990. Spawning sites covered about 10.5 ha in 1989 and 13.5 ha in 1990. Fish spawned as river temperature increased from 9.6 to 14.0°C and river discharge decreased from 390 to 240 m 3 /s. Physical characteristics of spawning sites were boulder–rubble substrate, water depth of 1.8–5.5 m, and bottom water velocity of 0.3–0.7 m/s. We captured no ovulating females but verified successful spawning in 1990 by capturing two live embryos. Gill‐net captures and telemetry during spring showed that some males moved to the spawning area annually. The low abundance estimates of spawning fish (9 in 1989 and 16 in 1990) indicate that the shortnose sturgeon population in the Merrimack River is the smallest yet identified and is likely vulnerable to extirpation.

Massachusetts↗

Feeding response by northern squawfish to a hatchery release of juvenile salmonids in the Clearwater River, Idaho

We collected gut contents from northern squawfish Ptychocheilus oregonensis captured in the Clearwater River, Idaho, 0–6 km from its confluence with the Snake River, following the release of 1.1 million yearling chinook salmon Oncorhynchus tshawytscha from the Dworshak National Fish Hatchery. Before the hatchery release, northern squawfish gut contents (by weight) in the study area were 38% crayfish Pacifastacus spp., 26% insects, 19% nonsalmonid fish, and 16% wheat kernels Triticum spp. Juvenile salmonids constituted 54% of gut contents about 24 h after the hatchery release, 78% after 5 d, and 86% after 7 d. The mean number of salmonids per gut (1.2) after release was higher than typically seen in guts from northern squawfish collected in mid-reservoir areas away from hydroelectric dams on the Snake and Columbia rivers. Length-frequency distributions of juvenile salmonids eaten and those captured in a scoop trap 4 km upstream of the study area indicated that northern squawfish were selectively feeding on the smaller individuals. We attribute the high rates of predation in the study area to the artificially high density of juvenile salmonids resulting from the hatchery release and to the physical characteristics of the study area in which the river changed from free flowing to impounded. Our results suggest that northern squawfish can quickly exploit hatchery releases of juvenile salmonids away from release sites in the Columbia River basin.

Idaho↗

Comparison of three nonlinear models to describe long-term tag shedding by lake trout

We estimated long-term tag-shedding rates for lake trout Salvelinus namaycush using two existing models and a model we developed to account for the observed permanence of some tags. Because tag design changed over the course of the study, we examined tag-shedding rates for three types of numbered anchor tags (Floy tags FD-67, FD-67C, and FD-68BC) and an unprinted anchor tag (FD-67F). Lake trout from the Gull Island Shoal region, Lake Superior, were double-tagged, and subsequent recaptures were monitored in annual surveys conducted from 1974 to 1992. We modeled tag-shedding rates, using time at liberty and probabilities of tag shedding estimated from fish released in 1974 and 1978&ndash;1983 and later recaptured. Long-term shedding of numbered anchor tags in lake trout was best described by a nonlinear model with two parameters: an instantaneous tag-shedding rate and a constant representing the proportion of tags that were never shed. Although our estimates of annual shedding rates varied with tag type (0.300 for FD-67, 0.441 for FD-67C, and 0.656 for FD-68BC), differences were not significant. About 36% of tags remained permanently affixed to the fish. Of the numbered tags that were shed (about 64%), two mechanisms contributed to tag loss: disintegration and dislodgment. Tags from about 11% of recaptured fish had disintegrated, but most tags were dislodged. Unprinted tags were shed at a significant but low rate immediately after release, but the long-term, annual shedding rate of these tags was only 0.013. Compared with unprinted tags, numbered tags dislodged at higher annual rates; we hypothesized that this was due to the greater frictional drag associated with the larger cross-sectional area of numbered tags.

Transactions of the American Fisheries Society↗