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α-Radioactivity of cerium-142

JOHNSON AND NIER 1 have measured the atomic masses of some of the rare-earth isotopes and have shown that the mass difference cerium-142—(barium-138 + helium-4) is equivalent to 1.68 ± 0.10 MeV. Similar results for the naturally occurring samarium and neodymium isotopes show that the α -active isotope of each element is the one having the largest possible decay energy. Rasmussen and others 2 suggest that the two or three neutrons just beyond the closed shell of 82 neutrons have decreased binding energies and hence the α -energy has a maximum about 84 neutrons. Johnson and Nier suggest that the α -decay of cerium-142 may take place with enough energy to be experimentally observable. Porschen and Riezler 3 examined a sample of un-enriched cerium ammonium citrate using nuclear track plates sensitive to α -particles. No α -activity was observed after a 30-day exposure of 1.2 mgm. of the cerium salt. In 1957 Riezler and Kauw 4 reported an alpha activity for an enriched sample of cerium-142. From their results they calculated a half-life of 5.1 × 10 15 years with an uncertainty factor of 2.

Nature↗

USGS Workshop on Scientific Aspects of a Long-Term Experimental Plan for Glen Canyon Dam, April 10-11, 2007, Flagstaff, Arizona

Executive Summary Glen Canyon Dam is located in the lower reaches of Glen Canyon National Recreation Area on the Colorado River, approximately 15 miles upriver from Grand Canyon National Park (fig. 1). In 1992, Congress passed and the President signed into law the Grand Canyon Protection Act (GCPA; title XVIII, sec. 1801?1809, of Public Law 102-575), which seeks ?to protect, mitigate adverse impacts to, and improve the values for which Grand Canyon National Park and Glen Canyon National Recreation Area were established.? The Glen Canyon Dam Adaptive Management Program (GCDAMP) was implemented as a result of the 1996 Record of Decision on the Operation of Glen Canyon Dam Final Environmental Impact Statement to ensure that the primary mandate of the GCPA is met through advances in information and resources management (U.S. Department of the Interior, 1995). On November 3, 2006, the Bureau of Reclamation (Reclamation) announced it would develop a long-term experimental plan environmental impact statement (LTEP EIS) for operational activities at Glen Canyon Dam and other management actions on the Colorado River. The purpose of the long-term experimental plan is twofold: (1) to increase the scientific understanding of the ecosystem and (2) to improve and protect important downstream resources. The proposed plan would implement a structured, longterm program of experimentation to include dam operations, potential modifications to Glen Canyon Dam intake structures, and other management actions such as removal of nonnative fish species. The development of the long-term experimental plan continues efforts begun by the GCDAMP to protect resources downstream of Glen Canyon Dam, including Grand Canyon, through adaptive management and scientific experimentation. The LTEP EIS will rely on the extensive scientific studies that have been undertaken as part of the adaptive management program by the U.S. Geological Survey?s (USGS) Grand Canyon Monitoring and Research Center (GCMRC), one of the four research stations within the USGS Southwest Biological Science Center. On April 10 and 11, 2007, at the behest of Reclamation, the GCMRC convened a workshop with scientific experts to identify one or more scientifically credible, long-term experimental options for Reclamation to consider for the LTEP EIS that would be consistent with the purpose and need for the plan. Workshop participants included government, academic, and private scientists with broad experience in the Colorado River in Grand Canyon and regulated rivers around the world. Resource managers and GCDAMP participants were also present on the second day of the workshop. In advance of the workshop, Reclamation and LTEP EIS cooperating agencies identified 14 core scientific questions. Workshop participants were asked to consider how proposed options would address these questions, which fall primarily into four areas: (1) conservation of endangered humpback chub (Gila cypha) and other high-priority biological resources, (2) conservation of sediment resources, (3) enhancement of recreational resources, and (4) preservation of cultural resources. A secondary objective of the workshop was the evaluation of four long-term experimental options developed by the GCDAMP Science Planning Group (SPG) (appendix B). The flow and nonflow treatments called for in the four experimental options were an important starting point for workshop discussions. At the beginning of the workshop, participants were provided with the final LTEP EIS scoping report prepared by Reclamation. Participants were also advised that Reclamation had committed to ?make every effortEto ensure that a new population of humpback chub is established in the mainstem or one or more of the tributaries within Grand Canyon? in the 1995 Operation of Glen Canyon Dam Final Environmental Impact Statement (U.S. Department of the Interior, 1995). This decision was consistent with the U.S. Fish and Wildlife Service?s 1995 bi

Open-File Report↗

Determination of nitrogen in coal macerals using electron microprobe technique-experimental procedure

This paper discusses nitrogen determination with the Cameca SX50 electron microprobe using PCO as an analyzing crystal. A set of conditions using differing accelerating voltages, beam currents, beam sizes, and counting times were tested to determine parameters that would give the most reliable nitrogen determination. The results suggest that, for the instrumentation used, 10 kV, current 20 nA, and a counting time of 20 s provides the most reliable nitrogen determination, with a much lower detection limit than the typical concentration of this element in coal. The study demonstrates that the electron microprobe technique can be used to determine the nitrogen content of coal macerals successfully and accurately. ?? 2001 Elsevier Science B.V. All rights reserved.

International Journal of Coal Geology↗

Fitness homeostasis across an experimental water gradient predicts species' geographic range and climatic breadth

Species range sizes and realized niche breadths vary tremendously. Understanding the source of this variation has been a long-term aim in evolutionary ecology and is a major tool in efforts to ameliorate the impacts of changing climates on species distributions. Species ranges that span a large climatic envelope can be achieved by a collection of specialized genotypes locally adapted to a small range of conditions, by genotypes with stable fitness across variable environments, or a combination of these factors. We asked whether fitness expressed along a key niche axis, water availability, could explain a species' realized niche breadth, its geographic range and climate breadth, in 11 species from a clade of jewelflowers whose range sizes vary by two orders of magnitude. Specifically, we explored whether the range size of a species was related to the ability of genotypes (maternal families) to maintain fitness across a range of experimental water availabilities based on 30-year historical field precipitation regimes. We operationally characterized fitness homeostasis through the coefficient of variation in fitness of a genotype (family) across the experimental water gradient. We found that species with genotypes that had high fitness homeostasis, low variation in fitness over our treatments, had larger climatic niche breadth and geographic range in their field distributions. The result was robust to alternate measures of fitness homeostasis. Our results show that the fitness homeostasis of genotypes can be a major factor contributing to niche breadth and range size in this clade. Fitness homeostasis can buffer species from loss of genetic diversity and under changing climates, provides time for adaptation to future conditions.

Ecology↗

Effects of behavioral and morphological plasticity on risk of predation in a Neotropical tadpole

Predator-induced phenotypic plasticity is widespread among aquatic animals, however the relative contributions of behavioral and morphological shifts to reducing risk of predation remain uncertain. We tested the phenotypic plasticity of a Neotropical tadpole (Rana palmipes) in response to chemical cues from predatory Belostoma water bugs, and how phenotype affects risk of predation. Behavior, morphology, and pigmentation all were plastic, resulting in a predator-induced phenotype with lower activity, deeper tail fin and muscle, and darker pigmentation. Tadpoles in the predator cue treatment also grew more rapidly, possibly as a result of the nutrient subsidy from feeding the caged predator. For comparison to phenotypes induced in the experiment, we quantified the phenotype of tadpoles from a natural pool. Wildcaught tadpoles did not match either experimentally induced phenotype; their morphology was more similar to that produced in the control treatment, but their low swimming activity was similar to that induced by predator cues. Exposure of tadpoles from both experimental treatments and the natural pool to a free-ranging predator confirmed that predator-induced phenotypic plasticity reduces risk of predation. Risk of predation was comparable among wild-caught and predator-induced tadpoles, indicating that behavioral shifts can substantially alleviate risk in tadpoles that lack the typical suite of predator-induced morphological traits. The morphology observed in wild-caught tadpoles is associated with rapid growth and high competition in other tadpole species, suggesting that tadpoles may profitably combine a morphology suited to competition for food with behaviors that minimize risk of predation. ?? Springer-Verlag 2004.

Oecologia↗

The effects of estradiol-17β on the sex reversal, survival, and growth of green sunfish Lepomis cyanellus

The feminization of green sunfish Lepomis cyanellus could expand their utility as a game fish or aquacultured species by preventing overcrowding and precocious reproduction in stocked systems. Feminization of green sunfish could also help elucidate information on their sex determination system. We report the feminization of green sunfish cohorts via oral administration of estradiol-17β (E2) during early development. A low-dose (100 E2 mg per kg of diet) and a high-dose (150 E2 mg per kg of diet) experimental E2 treatment were fed to juvenile green sunfish from 30 to 90 days post-hatch. Fish were subsequently evaluated for any treatment effect on gonadal development, survival, and growth. Both E2 treatments resulted in 100% feminization, with no morphological or histological differences detected between E2 treated ovaries and those from a control group. The control group was composed mostly of males (82.61%). Overall, there was no effect of E2 on survival ( P = 0.310) and growth rate data suggested no statistical differences ( P = 0.0805). However, the growth rate of the high-dose group increased slightly higher after the treatment ended than the other treatments ( P = 0.042), suggesting that E2 might suppress growth in green sunfish. In addition, the control group did not exhibit a higher survival rate after the treatment period ended ( P = 0.266), whereas both E2 treated groups did ( P = 0.0003–0.0050). We found that the low dose, 100 E2 mg per kg of diet, was sufficient for fully feminizing green sunfish if administered during development from 30 to 90 days post-hatch and E2 dosages may result in deleterious effects on green sunfish's health and growth.

Aquaculture↗

Incubation stage and polychlorinated biphenyl (PCB) congener patterns in an altricial and precocial bird species

The composition of polychlorinated biphenyl (PCB) congeners was compared between non-incubated and embryonated eggs of tree swallows ( Tachycineta bicolor ) and little terns ( Sterna albifrons ) to determine if measurable changes in PCB congeners occurred during the embryonic period. There was no indication of changes in PCB congener patterns over the incubation period in tree swallows in 1999 and 2000 at a site with very high PCB exposure or a site with more modest PCB exposure. Additionally, congeners known to be either quickly metabolized or conserved based on experimental studies did not generally respond as predicted. Similarly, PCB congener patterns in eggs of little terns from Bottsand, Schleswig-Holstein, Germany, did not differ between non-incubated and embryonated eggs. The results from both species suggest that the stage of incubation is not an important consideration when evaluating PCB congener patterns; comparisons and assessments can be made with eggs collected at all stages of incubation.

Environmental Pollution↗

Gut microbiome composition associates with corticosteroid treatment, morbidity, and senescence in Chinook salmon (Oncorhynchus tshawytscha)

Pacific salmon experience prolonged elevation in corticosteroid hormones during important life history events including migration, reproduction, and senescence. These periods of elevated corticosteroids correspond with changes to immunity and energy metabolism; therefore, fish may be particularly vulnerable to mortality at these times. Recent studies found that stress-induced cortisol release associated with microbial community shifts in salmonids, raising the question of how longer-term corticosteroid dynamics that accompany life history transitions affect salmonid microbiomes. In this work, we experimentally evaluated the relationships between gut microbiome composition, chronically elevated corticosteroids, and mortality in juvenile Chinook salmon ( Oncorhynchus tshawytscha) . We found that treatment with slow-release implants of the corticosteroids cortisol or dexamethasone resulted in persistent changes to the gut microbiome. Morbidity was also associated with microbiome composition, suggesting that the gut microbiome reflects individual differences in susceptibility to opportunistic pathogens. Additionally, we analyzed a small number of samples from adult fish at various stages of senescence. Results from these samples suggest that microbiome composition associated with gut integrity, and that the microbial communities of corticosteroid treated juveniles shift in composition toward those of senescent adults. Overall, findings from this work suggest that the gut microbiome associates with mortality risk during periods of chronic corticosteroid elevation.

Scientific Reports↗

Experimental challenge and pathology of highly pathogenic avian influenza virus H5N1 in dunlin ( Calidris alpina ), an intercontinental migrant shorebird species

Background Shorebirds (Charadriiformes) are considered one of the primary reservoirs of avian influenza. Because these species are highly migratory, there is concern that infected shorebirds may be a mechanism by which highly pathogenic avian influenza virus (HPAIV) H5N1 could be introduced into North America from Asia. Large numbers of dunlin ( Calidris alpina ) migrate from wintering areas in central and eastern Asia, where HPAIV H5N1 is endemic, across the Bering Sea to breeding areas in Alaska. Low pathogenic avian influenza virus has been previously detected in dunlin, and thus, dunlin represent a potential risk to transport HPAIV to North America. To date no experimental challenge studies have been performed in shorebirds. Methods Wild dunlin were inoculated intranasally and intrachoanally various doses of HPAIV H5N1. The birds were monitored daily for virus excretion, disease signs, morbidity, and mortality. Results The infectious dose of HPAIV H5N1 in dunlin was determined to be 10 1.7 EID 50 /100 μl and that the lethal dose was 10 1.83 EID 50 /100 μl. Clinical signs were consistent with neurotropic disease, and histochemical analyses revealed that infection was systemic with viral antigen and RNA most consistently found in brain tissues. Infected birds excreted relatively large amounts of virus orally (10 4 EID 50 ) and smaller amounts cloacally. Conclusions Dunlin are highly susceptible to infection with HPAIV H5N1. They become infected after exposure to relatively small doses of the virus and if they become infected, they are most likely to suffer mortality within 3–5 days. These results have important implications regarding the risks of transport and transmission of HPAIV H5N1 to North America by this species and raises questions for further investigation.

Alaska↗

Effects of exercise and bioprocessed soybean meal diets during rainbow trout rearing

Background: Alternative protein sources to fishmeal in fish feeds are needed. Objectives: Evaluate rearing performance of adult rainbow trout (Oncorhynchus mykiss) (initial weight 139.0 ±1.5 g, length 232.9 ± 0.8 mm, mean ± SE) fed one of the two isonitrogenous and isocaloric diets (46% protein, 16% lipid) and reared at one of the two levels of exercise (water velocities of either 3.6 cm/s or 33.2 cm/s). Methods: Protein in the control diet was based on fishmeal. In the experimental diet, bioprocessed soybean meal replaced approximately 60% of the fishmeal. Fish were fed by hand once-per-day to near satiation, and the food was increased daily. The experiment lasted 90-days. Results: There were no significant differences in gain, percent gain, or specific growth rate between the dietary treatments. However, the amount of food fed and feed conversion ratio was significantly lower in the 60% bioprocessed soybean meal diet. Intestinal morphology, relative fin length, splenosomatic index, hepatosomatic index, and viscerosomatic index were not significantly different in the trout fed either diet. Fish reared at 3.6 cm/s had a significantly lower feed conversion ratio (1.02 ± 0.02) than fish reared at 33.2 cm/s (1.13 ± 0.02). However, there were no significant differences in gain, percent gain, specific growth rate, or percentage mortality in fish reared with or without exercise. No significant interactions were observed between diet and exercise (higher water velocity). Conclusion:Based on these results, at least 60% of the fishmeal in adult rainbow trout diets can be replaced by bioprocessed soybean meal, even if higher water velocities are used to exercise the fish.

The Open Biology Journal↗

Quantifying the stormwater runoff volume reduction benefits of urban street tree canopy

Trees in the urban right-of-way areas have increasingly been considered part of a suite of green infrastructure practices used to manage stormwater runoff. A paired-catchment experimental design (with street tree removal as the treatment) was used to assess how street trees affect major hydrologic fluxes in a typical residential stormwater collection and conveyance network. The treatment consisted of removing 29 green ash (Fraxinus pennsylvanica) and two Norway maple (Acer platanoides) street trees from a medium-density residential area. Tree removal resulted in an estimated 198 m3 increase in surface runoff volume compared to the control catchment over the course of the study. This increase accounted for 4% of the total measured runoff after trees were removed. Despite significant changes to runoff volume (p ≤ 0.10), peak discharge was generally not affected by tree removal. On a per-tree basis, 66 L of rainfall per m2 of canopy was lost that would have otherwise been intercepted and stored. Runoff volume reduction benefit was estimated at 6376 L per tree. These values experimentally document per-capita retention services rendered by trees over a growing season with 42 storm events. These values are within the range reported by previous studies, which largely relied on simulation. This study provides catchment scale evidence that reducing stormwater runoff is one of many ecosystem services provided by street trees. This study quantifies these services, based on site conditions and a mix of deciduous species, and serves to improve our ability to account for this important yet otherwise poorly constrained hydrologic service. Engineers, city planners, urban foresters, and others involved with the management of urban stormwater can use this information to better understand tradeoffs involved in using green infrastructure to reduce urban runoff burden.

Wisconsin↗

Effect of heat and singeing on stable hydrogen isotope ratios of bird feathers and implications for their use in determining geographic origin

Rationale Stable hydrogen isotope ( δ 2 H) ratios of animal tissues are useful for assessing movement and geographic origin of mobile organisms. However, it is uncertain whether heat and singeing affects feather δ 2 H values and thus subsequent geographic assignments. This is relevant for birds of conservation interest that are burned and killed at concentrating solar‐energy facilities that reflect sunlight to a receiving tower and generate a solar flux field. Methods We used a controlled experiment to test the effect of known heat loads (exposure to 200, 250 or 300°C for 1 min) on the morphology and δ 2 H values of feathers from two songbird species. Subsequently, we examined the effects of singeing on δ 2 H values of feathers from three other songbird species that were found dead in the field at a concentrating solar‐energy facility. Results Relative to control samples, heating caused visual morphological changes to feathers, including shriveling at 250°C and charring at 300°C. The δ 2 H values significantly declined by a mean of 27.8‰ in experimental samples exposed to 300°C. There was no statistically detectable difference between δ 2 H values of the singed and unsinged portions of field‐collected feathers from the same bird. Conclusions Limited singeing that did not dramatically alter the feather morphology did not substantially affect δ 2 H values of feathers from these songbirds. However, higher temperatures induced charring and reduced δ 2 H values. Therefore, severely charred feathers should be avoided when selecting feathers for δ 2 H‐based assessment of geographic origin.

Rapid Communications in Mass Spectrometry↗

Overwinter and prespawning movements by a vulnerable freshwater pelagophilic minnow

The decline of pelagophil minnows is related to river fragmentation across the southern Great Plains landscape. Because we know little about pelagophil movement patterns and timing, we aimed to quantify the movements of the vulnerable Arkansas River shiner (ARS) during the winter (November–March) and prespawning (April–June) seasons. We tagged 4233 ARS using visible implant elastomer, passive integrated transponder, or p-Chip micro-transponder tags in 2018–2020. We sampled to recapture tagged fish weekly during the winter and biweekly during the spring. Tagged fish exhibited a downstream movement bias and movement was weakly related to increasing temperature, discharge, and photoperiod during winter, however most of the variability was explained by a random individual effect. Larger individuals moved greater distances than smaller fish. Upstream movements by a migratory portion of the population appeared to begin around late February based on the presence of fish at previously unoccupied sites. However, the first long-distance (30-km) upstream movement by a tagged fish was documented in late May. We show evidence that some ARS may be resident fish at sites throughout winter and spring of multiple years. To conserve freshwater pelagophil minnows, our results indicate water management strategies improving river connectivity in late winter through the spawning season may benefit spawning by migratory individuals, whereas lateral connectivity might benefit the resident portion of the population. Research efforts under experimental flows could provide insight to improved recovery options.

Oklahoma↗

Cytonuclear genetics of experimental fish hybrid zones inside Biosphere 2

Two species of mosquitofish (family Poeciliidae ) known to hybridize in nature were introduced into freshwater habitats inside Biosphere 2, and their population genetics were monitored after 2 years. Within four to six generations, nuclear and cytoplasmic markers characteristic of Gambusia holbrooki had risen greatly in frequency, although some Gambusia affinis alleles and haplotypes were retained primarily in recombinant genotypes, indicative of introgressive hybridization. The temporal cytonuclear dynamics proved similar to population genetic changes observed in replicated experimental hybrid populations outside of Biosphere 2, thus indicating strong directional selection favoring G. holbrooki genotypes across the range of environments monitored. When interpreted in the context of species-specific population demographies observed previously, results suggest that the extremely rapid evolution in these zones of secondary contact is attributable primarily to species differences in life-history traits.

PNAS↗

Effects of resource chemistry on the composition and function of stream hyporheic biofilms.

Fluvial ecosystems process large quantities of dissolved organic matter as it moves from the headwater streams to the sea. In particular, hyporheic sediments are centers of high biogeochemical reactivity due to their elevated residence time and high microbial biomass and activity. However, the interaction between organic matter and microbial dynamics in the hyporheic zone remains poorly understood. We evaluated how variance in resource chemistry affected the microbial community and its associated activity in experimentally grown hyporheic biofilms. To do this we fed beech leaf leachates that differed in chemical composition to a series of bioreactors filled with sediment from a sub-alpine stream. Differences in resource chemistry resulted in differences in diversity and phylogenetic origin of microbial proteins, enzyme activity, and microbial biomass stoichiometry. Specifically, increased lignin, phenolics, and manganese in a single leachate resulted in increased phenoloxidase and peroxidase activity, elevated microbial biomass carbon:nitrogen ratio, and a greater proportion of proteins of Betaproteobacteria origin. We used this model system to attempt to link microbial form (community composition and metaproteome) with function (enzyme activity) in order to better understand the mechanisms that link resource heterogeneity to ecosystem function in stream ecosystems.

Frontiers in Microbiology↗

Detecting cover crop end-of-season using VENµS and sentinel-2 satellite imagery

Cover crops are planted during the off-season to protect the soil and improve watershed management. The ability to map cover crop termination dates over agricultural landscapes is essential for quantifying conservation practice implementation, and enabling estimation of biomass accumulation during the active cover period. Remote sensing detection of end-of-season (termination) for cover crops has been limited by the lack of high spatial and temporal resolution observations and methods. In this paper, a new within-season termination (WIST) algorithm was developed to map cover crop termination dates using the Vegetation and Environment monitoring New Micro Satellite (VENµS) imagery (5 m, 2 days revisit). The WIST algorithm first detects the downward trend (senescent period) in the Normalized Difference Vegetation Index (NDVI) time-series and then refines the estimate to the two dates with the most rapid rate of decrease in NDVI during the senescent period. The WIST algorithm was assessed using farm operation records for experimental fields at the Beltsville Agricultural Research Center (BARC). The crop termination dates extracted from VENµS and Sentinel-2 time-series in 2019 and 2020 were compared to the recorded termination operation dates. The results show that the termination dates detected from the VENµS time-series (aggregated to 10 m) agree with the recorded harvest dates with a mean absolute difference of 2 days and uncertainty of 4 days. The operational Sentinel-2 time-series (10 m, 4–5 days revisit) also detected termination dates at BARC but had 7% missing and 10% false detections due to less frequent temporal observations. Near-real-time simulation using the VENµS time-series shows that the average lag times of termination detection are about 4 days for VENµS and 8 days for Sentinel-2, not including satellite data latency. The study demonstrates the potential for operational mapping of cover crop termination using high temporal and spatial resolution remote sensing data.

Remote Sensing↗

The influence of precipitation, vegetation and soil properties on the ecohydrology of sagebrush steppe rangelands on the INL site

The INL Site and other landscapes having sagebrush steppe vegetation are experiencing a simultaneous change in climate and floristics that result from increases in exotic species. Determining the separate and combined/interactive effects of climate and vegetation change is important for assessing future changes on the landscape and for hydrologic processes. This research uses the 72 experimental plots established and initially maintained for many years as the “Protective Cap Biobarrier Experiment” by Dr. Jay Anderson and the Stoller ESER program, and the experiment is also now referred to as the “INL Site Ecohydrology Study.” We are evaluating long-term impacts of different plant communities commonly found throughout Idaho subject to different precipitation regimes and to different soil depths. Treatments of amount and timing of precipitation (irrigation), soil depth, and either native/perennial or exotic grass vegetation allow researchers to investigate how vegetation, precipitation and soil interact to influence soil hydrology and ecosystem biogeochemistry. This information will be used to improve a variety of models, as well as provide data for these models.

Report↗

Proceedings of the Fiscal Year 2022 Annual Reporting Meeting to the Glen Canyon Dam Adaptive Management Program

(Hartwell) This report is prepared primarily to account for work conducted and products delivered in FY 2022 by GCMRC and to inform the Technical Work Group of science conducted by GCMRC and its cooperators in support of the Glen Canyon Dam Adaptive Management Program (GCDAMP). It includes a summary of accomplishments, modifications to work plans, results, and recommendations related to projects included in GCMRC’s FY 2021-23 Triennial Work Plan. This work was done to support the 11 resource goals identified in the Glen Canyon Dam Long-Term Experimental and Management Plan (LTEMP) Environmental Impact Statement and Record of Decision (Table 1).

Arizona↗