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Streamflow of 2014: Water year summary

The maps and graphs in this summary describe streamflow conditions for water year 2014 (October 1, 2013, to September 30, 2014) in the context of the 85-year period from 1930 through 2014, unless otherwise noted. The illustrations are based on observed data from the U.S. Geological Survey’s (USGS) National Streamflow Information Program (NSIP) ( http://water.usgs.gov/nsip/ ). The period 1930–2014 was used because, prior to 1930, the number of streamgages was too small to provide representative data for computing statistics for most regions of the country. In the summary, reference is made to the term “runoff,” which is the depth to which a river basin or other geographic area, such as a State, would be covered with water if all the streamflow within the area during a specified time period was uniformly distributed over the area. Runoff can also be used to quantify the magnitude of water flowing through rivers and streams in measurement units that can be compared from one area of the Nation to another.

Fact Sheet

Patterns of den occupation by the spotted hyaena (Crocuta crocuta)

Spotted hyaenas utilize isolated natal dens (NDs) and communal dens (CDs) for rearing their cubs. Here we describe patterns of natal and CD occupation by hyaenas belonging to one well-studied clan in the Maasai Mara National Reserve during a 10-year period. Locations of 98 den sites that were used as natal or CDs by hyaenas in the study clan were digitized in a Geographic Information System, and the duration of use of each den site, frequency of re-use, and distances involved in den moves were quantified. Hyaenas moved their CD monthly on average. Most CD sites were occupied only once during the study, but several sites were used repeatedly. On rare occasions, the movement of hyaenas to a new den site could be attributed to a disturbance event at the CD, but factors regularly prompting hyaenas to move to new CD sites were unclear. High-ranking female hyaenas were more likely to rear their cubs from birth in a CD than low-ranking females. Low-ranking females almost always utilized isolated NDs for the first few weeks of a litter's development, and low-ranking females transferred their cubs over longer distances than did high-ranking females. ?? 2006 East African Wild Life Society.

African Journal of Ecology

Forecasting weed distributions using climate data: a GIS early warning tool

The number of invasive exotic plant species establishing in the United States is continuing to rise. When prevention of exotic species from entering into a country fails at the national level and the species establishes, reproduces, spreads, and becomes invasive, the most successful action at a local level is early detection followed eradication. We have developed a simple geographic information system (GIS) analysis for developing watch lists for early detection of invasive exotic plants that relies upon currently available species distribution data coupled with environmental data to aid in describing coarse-scale potential distributions. This GIS analysis tool develops environmental envelopes for species based upon the known distribution of a species thought to be invasive and represents the first approximation of its potential habitat while the necessary data are collected to perform more in­-depth analyses. To validate this method we looked at a time series of species distributions for 66 species in Pacific Northwest, and northern Rocky Mountain counties. The time series analysis presented here did select counties that the invasive exotic weeds invaded in subsequent years, showing that this technique could be useful in developing watch lists for the spread of particular exotic species. We applied this same habitat-matching model based upon bioclimaric envelopes to 100 invasive exotics with various levels of known distributions within continental U.S. counties. For species with climatically limited distributions, county watch lists describe county-specific vulnerability to invasion. Species with matching habitats in a county would be added to that county's list. These watch lists can influence management decisions for early warning, control prioritization, and targeted research to determine specific locations within vulnerable counties. This tool provides useful information for rapid assessment of the potential distribution based upon climate envelopes of current distributions for new invasive exotic species.

Invasive Plant Science and Management

Hydrologic and geologic aspects of waste management and disposal; a bibliography of publications by U.S. Geological Survey authors, 1950-81

References to more than 550 reports, articles, and maps are listed alphabetically by author and are indexed by subject. The subject index includes geographic-area terms. Citations from 69 series are included; series are listed separately. The publications listed report the results of U.S. Geological Survey research and field projects throughout the Nation concerning earth-science aspects of waste management and disposal. They include organic, inorganic, and radioactive wastes and related topics such as mathematical models of solute transport. Most of the references are to (1) Geological Survey report series such as Water-Supply Papers, Professional Papers, Bulletins, Circulars, Water-Resources Investigations, and Open-File Reports, (2) technical journals of professional organizations, or (3) reports by other Federal and State agencies.

Circular

Enhanced Historical Land-Use and Land-Cover Data Sets of the U.S. Geological Survey

Historical land-use and land-cover data, available from the U.S. Geological Survey (USGS) for the conterminous United States and Hawaii, have been enhanced for use in geographic information systems (GIS) applications. The original digital data sets were created by the USGS in the late 1970s and early 1980s and were later converted by USGS and the U.S. Environmental Protection Agency (USEPA) to a geographic information system (GIS) format in the early 1990s. These data were made available on USEPA's Web site since the early 1990s and have been used for many national applications, despite minor coding and topological errors. During the 1990s, a group of USGS researchers made modifications to the data set for use in the National Water-Quality Assessment Program. These edited files have been further modified to create a more accurate, topologically clean, and seamless national data set. Several different methods, including custom editing software and several batch processes, were applied to create this enhanced version of the national data set. The data sets are included in this report in the commonly used shapefile and Tagged Image Format File (TIFF) formats. In addition, this report includes two polygon data sets (in shapefile format) representing (1) land-use and land-cover source documentation extracted from the previously published USGS data files, and (2) the extent of each polygon data file.

Data Series

Hydrogeomorphic Classification of Wetlands on Mt. Desert Island, Maine, Including Hydrologic Susceptibility Factors for Wetlands in Acadia National Park

The U.S. Geological Survey, in cooperation with the National Park Service, developed a hydrogeomorphic (HGM) classification system for wetlands greater than 0.4 hectares (ha) on Mt. Desert Island, Maine, and applied this classification using map-scale data to more than 1,200 mapped wetland units on the island. In addition, two hydrologic susceptibility factors were defined for a subset of these wetlands, using 11 variables derived from landscape-scale characteristics of the catchment areas of these wetlands. The hydrologic susceptibility factors, one related to the potential hydrologic pathways for contaminants and the other to the susceptibility of wetlands to disruptions in water supply from projected future changes in climate, were used to indicate which wetlands (greater than 1 ha) in Acadia National Park (ANP) may warrant further investigation or monitoring. The HGM classification system consists of 13 categories: Riverine-Upper Perennial, Riverine-Nonperennial, Riverine- Tidal, Depressional-Closed, Depressional-Semiclosed, Depressional-Open, Depressional-No Ground-Water Input, Mineral Soil Flat, Organic Soil Flat, Tidal Fringe, Lacustrine Fringe, Slope, and Hilltop/Upper Hillslope. A dichotomous key was developed to aid in the classification of wetlands. The National Wetland Inventory maps produced by the U.S. Fish and Wildlife Service provided the wetland mapping units used for this classification. On the basis of topographic map information and geographic information system (GIS) layers at a scale of 1:24,000 or larger, 1,202 wetland units were assigned a preliminary HGM classification. Two of the 13 HGM classes (Riverine-Tidal and Depressional-No Ground-Water Input) were not assigned to any wetlands because criteria for determining those classes are not available at that map scale, and must be determined by more site-specific information. Of the 1,202 wetland polygons classified, which cover 1,830 ha in ANP, 327 were classified as Slope, 258 were Depressional (Open, Semiclosed, and Closed), 231 were Riverine (Upper Perennial and Nonperennial), 210 were Soil Flat (Mineral and Organic), 68 were Lacustrine Fringe, 51 were Tidal Fringe, 22 were Hilltop/Upper Hillslope, and another 35 were small open water bodies. Most small, isolated wetlands classified on the island are Slope wetlands. The least common, Hilltop/Upper Hillslope wetlands, only occur on a few hilltops and shoulders of hills and mountains. Large wetland complexes generally consist of groups of Depressional wetlands and Mineral Soil Flat or Organic Soil Flat wetlands, often with fringing Slope wetlands at their edges and Riverine wetlands near streams flowing through them. The two analyses of wetland hydrologic susceptibility on Mt. Desert Island were applied to 186 wetlands located partially or entirely within ANP. These analyses were conducted using individually mapped catchments for each wetland. The 186 wetlands were aggregated from the original 1,202 mapped wetland polygons on the basis of their HGM classes. Landscape-level hydrologic, geomorphic, and soil variables were defined for the catchments of the wetlands, and transformed into scaled scores from 0 to 10 for each variable. The variables included area of the wetland, area of the catchment, area of the wetland divided by the area of the catchment, the average topographic slope of the catchment, the amount of the catchment where bedrock crops out with no soil cover or excessively thin soil cover, the amount of storage (in lakes and wetlands) in the catchment, the topographic relief of the catchment, the amount of clay-rich soil in the catchment, the amount of manmade impervious surface, whether the wetland had a stream inflow, and whether the wetland had a hydraulic connection to a lake or estuary. These data were determined using a GIS and data layers mapped at a scale of 1:24,000 or larger. These landscape variables were combined in different ways for the two hydrologic susceptibility fact

Scientific Investigations Report

Near-real-time simulation and internet-based delivery of forecast-flood inundation maps using two-dimensional hydraulic modeling--A pilot study for the Snoqualmie River, Washington

A system of numerical hydraulic modeling, geographic information system processing, and Internet map serving, supported by new data sources and application automation, was developed that generates inundation maps for forecast floods in near real time and makes them available through the Internet. Forecasts for flooding are generated by the National Weather Service (NWS) River Forecast Center (RFC); these forecasts are retrieved automatically by the system and prepared for input to a hydraulic model. The model, TrimR2D, is a new, robust, two-dimensional model capable of simulating wide varieties of discharge hydrographs and relatively long stream reaches. TrimR2D was calibrated for a 28-kilometer reach of the Snoqualmie River in Washington State, and is used to estimate flood extent, depth, arrival time, and peak time for the RFC forecast. The results of the model are processed automatically by a Geographic Information System (GIS) into maps of flood extent, depth, and arrival and peak times. These maps subsequently are processed into formats acceptable by an Internet map server (IMS). The IMS application is a user-friendly interface to access the maps over the Internet; it allows users to select what information they wish to see presented and allows the authors to define scale-dependent availability of map layers and their symbology (appearance of map features). For example, the IMS presents a background of a digital USGS 1:100,000-scale quadrangle at smaller scales, and automatically switches to an ortho-rectified aerial photograph (a digital photograph that has camera angle and tilt distortions removed) at larger scales so viewers can see ground features that help them identify their area of interest more effectively. For the user, the option exists to select either background at any scale. Similar options are provided for both the map creator and the viewer for the various flood maps. This combination of a robust model, emerging IMS software, and application interface programming should allow the technology developed in the pilot study to be applied to other river systems where NWS forecasts are provided routinely.

Water-Resources Investigations Report

Digital line graphs from 1:24,000-scale maps

The Earth Science Information Centers (ESIC) distribute digital cartographic/geographic data files produced by the U.S. Geological Survey (USGS) as part of the National Mapping Program. Digital cartographic data flles are grouped into four basic types. The first of these, called a Digital Line . Graph (DLG), is line map information in digital form. These data files include information on planimetric base categories, such as transportation, hydrography, and boundaries. The second type, called a Digital Elevation Model (DEM), consists of a sampled array of elevations for a number of ground positions that are usually at regularly spaced intervals. The third type is Land Use and Land Cover digital data, which provides information on nine major classes of land use such as urban, agricultural, or forest as wen as associated map data such as political units and Federal land ownership. The fourth type, the Geographic Names Information System, provides primary information for all known places, features, and areas in the United States identified by a proper name.

Data Users Guide

Characterization of major-ion chemistry and nutrients in headwater streams along the Appalachian National Scenic Trail and within adjacent watersheds, Maine to Georgia

An inventory of water-quality data on field parameters, major ions, and nutrients provided a summary of water quality in headwater (first- and second-order) streams within watersheds along the Appalachian National Scenic Trail (Appalachian Trail). Data from 1,817 sampling sites in 831 catchments were used for the water-quality summary. Catchment delineations from NHDPlus were used as the fundamental geographic units for this project. Criteria used to evaluate sampling sites for inclusion were based on selected physical attributes of the catchments adjacent to the Appalachian Trail, including stream elevation, percentage of developed land cover, and percentage of agricultural land cover. The headwater streams of the Appalachian Trail are generally dilute waters, with low pH, low acid neutralizing capacity (ANC), and low concentrations of nutrients. The median pH value was slightly acidic at 6.7; the median specific conductance value was 23.6 microsiemens per centimeter, and the median ANC value was 98.7 milliequivalents per liter (μeq/L). Median concentrations of cations (calcium, magnesium, sodium, and potassium) were each less than 1.5 milligrams per liter (mg/L), and median concentrations of anions (bicarbonate, chloride, fluoride, sulfate, and nitrate) were less than 10 mg/L. Differences in water-quality constituent levels along the Appalachian Trail may be related to elevation, atmospheric deposition, geology, and land cover. Spatial variations were summarized by ecological sections (ecosections) developed by the U.S. Forest Service. Specific conductance, pH, ANC, and concentrations of major ions (calcium, chloride, magnesium, sodium, and sulfate) were all negatively correlated with elevation. The highest elevation ecosections (White Mountains, Blue Ridge Mountains, and Allegheny Mountains) had the lowest pH, ANC, and concentrations of major ions. The lowest elevation ecosections (Lower New England and Hudson Valley) generally had the highest pH, ANC, and concentrations of major ions. The geology in discrete portions of these two ecosections was classified as containing carbonate minerals which has likely influenced the chemical character of the streamwater. Specific conductance, pH, ANC, and concentrations of major ions (calcium, chloride, magnesium, sodium, and sulfate) were all positively correlated with percentages of developed and agricultural land uses at the lower elevations of the central region of the Appalachian Trail (including the Green-Taconic-Berkshire Mountains, Lower New England, Hudson Valley, and Northern Ridge and Valley ecosections). The distinctly different chemical character of the streams in the central sections of the Appalachian Trail is likely related to the lower elevations, the presence of carbonate minerals in the geology, higher percentages of developed and agricultural land uses, and possibly the higher inputs of sulfate and nitrate from atmospheric deposition. Acid deposition of sulfate and nitrate are important influences on the acid-base chemistry of the surface waters of the Appalachian Trail. Atmospheric deposition estimates are consistently high (more than 18 kilograms per hectare (kg/ha) for sulfate, and more than 16 kg/ha for nitrate) at both the highest and lowest elevations. However, the lowest elevation (Green-Taconic-Berkshire Mountains, Lower New England, Hudson Valley, Northern Glaciated Allegheny Plateau, and Northern Ridge and Valley ecosections) included the largest spatial area of sustained high estimates of atmospheric deposition. Calcium-bicarbonate was the most frequently calculated water type in the Lower New England and Hudson Valley ecosections. In the northern and southern sections of the Appalachian Trail mix-cation water types were most prevalent and sulfate was the predominate anion. The predominance of the sulfate anion in the surface waters of the northern and southern ecosections likely reflects the influence of sulfate deposition. Although the central portion of the Appalachian Trail has the largest spatial area of high atmospheric acid deposition, the lower ionic strength waters in the northern and southern ecosections of the Appalachian Trail may have been more adversely affected by acid deposition. The low ionic strength of the streams in the White Mountains, Blue Ridge Mountains, and Allegheny Mountains ecosections makes parts of these regions susceptible to seasonal or event-driven episodic acidification, which can be detrimental to health of aquatic and terrestrial ecosystems. Median catchment ANC values were classified into three groups - acidic, sensitive, and insensitive. The White Mountains, Blue Ridge Mountains, and Allegheny Mountains ecosections included the highest frequency of catchments classified as acidic or sensitive. More than 56 percent of the catchments from the White Mountains ecosection were classified as sensitive to acidic inputs. In the Blue Ridge ecosection, 1.6 percent of the catchments were classified as acidic, and 38.2 percent of the catchments were classified as sensitive to acidic inputs. In the Allegheny Mountains ecosection, 17.6 percent of the catchments were classified as acidic, and 29.4 percent of the catchments were classified as sensitive to acidic inputs. Median concentrations of nitrogen species were less than 0.4 mg/L, and median concentrations of total phosphorus were less than 0.02 mg/L along the Appalachian Trail. A comparison of median catchment concentrations of nutrients to estimated national background concentrations demonstrated that concentrations along the Appalachian Trail are generally lower. A comparison of median concentrations of total nitrogen and total phosphorus to the U.S. Environmental Protection Agency's (USEPA) nutrient criteria for the Eastern U.S. ecoregions showed that the concentrations of total nitrogen in the northern section of the Appalachian Trail were generally higher than the USEPA criterion. Similarly, median concentrations of total phosphorus in the southern regions of the Appalachian Trail were approximately twice as high as USEPA criteria. Sections of the Appalachian Trail are adjacent to modest amounts of agricultural and developed land areas. These nonforested land areas may be contributing to the percentage of catchments in which concentrations of total nitrogen and total phosphorus are higher than USEPA nutrient ecoregion criteria.

Connecticut, Georgia, Maine, Massachusetts, Maryla

Digital data in support of studies and assessments of coal and petroleum resources in the Appalachian basin

The Appalachian basin is a mature basin containing abundant oil, gas, and coal resources. Its fossil-fuel-bearing strata range in age from Cambrian to Permian and extend over the States of New York, Pennsylvania, Maryland, Ohio, West Virginia, Virginia, Kentucky, Tennessee, Georgia, and Alabama. The basin has provided abundant fossil fuels to support the Nation’s economic growth for at least 150 years and U.S. Geological Survey (USGS) assessments suggest that substantial untapped resources remain. A merger of new and old geologic data and ideas is required to locate and extract those remaining resources. This chapter brings together abundant existing datasets in a common and integrated spatial format to advance our understanding of the distribution, geologic framework, burial history, and geochemical character of the basin’s oil, gas, and coal resources. These datasets support the information and findings in chapters B through G of this volume, which include (1) geologic cross sections, seismic profiles, and burial history models that document the depositional and structural framework of the basin; (2) assessments of Carboniferous coal-bed gas and Devonian shale gas; (3) studies about the distribution of oil, gas, and coal fields and the geochemistry of natural gas and oil; and (4) a history of fossil-fuel production in the basin. The data that supported these studies were refined and made more accessible using geographic information system (GIS) tools. The following types of data are included: (1) the bedrock geology of States included within the boundary of the Appalachian basin, (2) the locations of oil and gas wells and fields, (3) data produced for the National Oil and Gas Assessment, and (4) the stratigraphy, geochemistry, and production of coal. Data layers are available in shapefile and Zipped Keyhole Markup Language (KMZ) formats, which can be viewed using ArcGIS, Google Earth, or any software package that supports these data formats. Metadata for these datasets are available in HTML and XML formats. Metadata files contain information about the sources of data used to create the dataset, the creation process steps, the data quality, the geographic coordinate system and horizontal datum used for the dataset, the values of attributes used in the dataset table, information about the publication and the publishing organization, and other information that may be useful to the reader. All links in the metadata were valid at the time of compilation. Some of these links may no longer be valid. No attempt has been made to determine the new online location (if one exists) for the data.

Appalachian basin

Mining Claim Activity on Federal Land in the United States

Several statistical compilations of mining claim activity on Federal land derived from the Bureau of Land Management's LR2000 database have previously been published by the U.S Geological Survey (USGS). The work in the 1990s did not include Arkansas or Florida. None of the previous reports included Alaska because it is stored in a separate database (Alaska Land Information System) and is in a different format. This report includes data for all states for which there are Federal mining claim records, beginning in 1976 and continuing to the present. The intent is to update the spatial and statistical data associated with this report on an annual basis, beginning with 2005 data. The statistics compiled from the databases are counts of the number of active mining claims in a section of land each year from 1976 to the present for all states within the United States. Claim statistics are subset by lode and placer types, as well as a dataset summarizing all claims including mill site and tunnel site claims. One table presents data by case type, case status, and number of claims in a section. This report includes a spatial database for each state in which mining claims were recorded, except North Dakota, which only has had two claims. A field is present that allows the statistical data to be joined to the spatial databases so that spatial displays and analysis can be done by using appropriate geographic information system (GIS) software. The data show how mining claim activity has changed in intensity, space, and time. Variations can be examined on a state, as well as a national level. The data are tied to a section of land, approximately 640 acres, which allows it to be used at regional, as well as local scale. The data only pertain to Federal land and mineral estate that was open to mining claim location at the time the claims were staked.

Data Series

EAARL coastal topography — Sandy Hook 2007

These remotely sensed, geographically referenced elevation measurements of Lidar-derived topography were produced as a collaborative effort between the U.S. Geological Survey (USGS), Florida Integrated Science Center (FISC), St. Petersburg, FL; the National Park Service (NPS), Northeast Coastal and Barrier Network, Kingston, RI; and the National Aeronautics and Space Administration (NASA), Wallops Flight Facility, VA. This project provides highly detailed and accurate datasets of Gateway National Recreation Area's Sandy Hook Unit in New Jersey, acquired on May 16, 2007. The datasets are made available for use as a management tool to research scientists and natural resource managers. An innovative airborne Lidar instrument originally developed at the NASA Wallops Flight Facility, and known as the Experimental Advanced Airborne Research Lidar (EAARL) was used during data acquisition. The EAARL system is a raster-scanning, waveform-resolving, green-wavelength (532-nanometer) Lidar designed to map near-shore bathymetry, topography, and vegetation structure simultaneously. The EAARL sensor suite includes the raster-scanning, water-penetrating full-waveform adaptive Lidar, a down-looking red-green-blue (RGB) digital camera, a high-resolution multi-spectral color infrared (CIR) camera, two precision dual-frequency kinematic carrier-phase GPS receivers and an integrated miniature digital inertial measurement unit, which provide for submeter georeferencing of each laser sample. The nominal EAARL platform is a twin-engine Cessna 310 aircraft, but the instrument may be deployed on a range of light aircraft. A single pilot, a Lidar operator, and a data analyst constitute the crew for most survey operations. This sensor has the potential to make significant contributions in measuring sub-aerial and submarine coastal topography within cross-environmental surveys. Elevation measurements were collected over the survey area using the EAARL system, and the resulting data were then processed using the Airborne Lidar Processing System (ALPS), a custom-built processing system developed in a NASA-USGS collaboration. ALPS supports the exploration and processing of Lidar data in an interactive or batch mode. Modules for pre-survey flight line definition, flight path plotting, Lidar raster and waveform investigation, and digital camera image playback have been developed. Processing algorithms have been developed to extract the range to the first and last significant return within each waveform. ALPS is routinely used to create maps that represent submerged or first surface topography. Specialized filtering algorithms have been implemented to determine the 'bare earth' under vegetation from a point cloud of last return elevations.

New York

Benthic habitat and geologic mapping of the outer continental shelf of north-central California

The Fanny Shoal area is located between North Farallon Island and Cordell Bank approximately 40 miles west of San Francisco, California. The area lies within the Gulf of the Farallones National Marine Sanctuary (GFNMS) which is located just a few miles from San Francisco. The waters within the GFNMS are part of a nationally significant marine ecosystem encompassing a diversity of highly productive marine habitats. Protection of the living and cultural resources at the sites are administered by the National Oceanic and Atmospheric Administration (NOAA). The U.S. Geological Survey (USGS) in cooperation with the Golden Gate National Recreation Area (GGNRA) and NOAA, including the GFNMS, and Monterey Bay National Marine Sanctuary (MBNMS), collected side-scanning sonar, and underwater video data over three cruises in July of 2003, and April of 2004. The data are consolidated into a geographic information system (GIS) to produce benthic habitat and geologic maps that provide researchers and those involved in decision making with crucial, georeferenced geologic information that will aid in preserving the area's environment.

California

Genetic variation underlying resistance to infectious hematopoietic necrosis virus in a steelhead trout ( Oncorhynchus mykiss ) population

Understanding the mechanisms of host resistance to pathogens will allow insights into the response of wild populations to the emergence of new pathogens. Infectious hematopoietic necrosis virus (IHNV) is endemic to the Pacific Northwest and infectious to Pacific salmon and trout ( Oncorhynchus spp.). Emergence of the M genogroup of IHNV in steelhead trout O. mykiss in the coastal streams of Washington State, between 2007 and 2011, was geographically heterogeneous. Differences in host resistance due to genetic change were hypothesized to be a factor influencing the IHNV emergence patterns. For example, juvenile steelhead trout losses at the Quinault National Fish Hatchery (QNFH) were much lower than those at a nearby facility that cultures a stock originally derived from the same source population. Using a classical quantitative genetic approach, we determined the potential for the QNFH steelhead trout population to respond to selection caused by the pathogen, by estimating the heritability for 2 traits indicative of IHNV resistance, mortality ( h 2 = 0.377 (0.226 - 0.550)) and days to death ( h 2 = 0.093 (0.018 - 0.203)). These results confirm that there is a genetic basis for resistance and that this population has the potential to adapt to IHNV. Additionally, genetic correlation between days to death and fish length suggests a correlated response in these traits to selection. Reduction of genetic variation, as well as the presence or absence of resistant alleles, could affect the ability of populations to adapt to the pathogen. Identification of the genetic basis for IHNV resistance could allow the assessment of the susceptibility of other steelhead populations.

Diseases of Aquatic Organisms

Eocene Yegua Formation (Claiborne group) and Jackson group lignite deposits of Texas

The lignite deposits within the upper Eocene Yegua Formation (Claiborne Group) and the overlying Jackson Group are among the coal resources that were not quantitatively assessed as part of the U.S. Geological Survey's (USGS) National Coal Resource Assessment (NCRA) program in the Gulf Coastal Plain coal province. In the past, these lignite-bearing stratigraphic units often have been evaluated together because of their geographic and stratigraphic proximity (Fisher, 1963; Kaiser, 1974; Kaiser et al., 1980; Jackson and Garner, 1982; Kaiser, 1996) (Figures 1, 2). The term “Yegua-Jackson trend“ is used informally herein for the lignite-bearing outcrops of these Late Eocene deposits in Texas. Lignite beds in the Yegua-Jackson trend generally are higher both in ash yield and sulfur content than those of the underlying Wilcox Group (Figure 2 ). Recent studies (Senkayi et al., 1987; Ruppert et al., 1994; Warwick et al., 1996, 1997) have shown that some lignite beds within the Yegua-Jackson trend contain partings of volcanic ash and host elevated levels of trace elements that have been identified as potentially hazardous air pollutants (HAPs) in the United States Clean Air Amendments of 1990. Lignite beds within the Yegua Formation are thin (less than or equal to 6 ft) and laterally discontinuous in comparison with most Wilcox Group deposits (Ayers, 1989a); in contrast, the Jackson Group lignite beds range up to 12 ft in total thickness and are relatively continuous laterally, extending nearly 32 mi along strike.

Texas

Using advanced population genomics to better understand the relationship between offshore and spawning habitat use for Atlantic Sturgeon

Atlantic Sturgeon ( Acipenser oxyrinchus oxyrinchus ) are a large-bodied anadromous fish that historically supported important fisheries along the east coast of the United States. Following years of overharvest and habitat degradation, populations experienced severe declines. In 2012, the National Marine Fisheries Service listed Atlantic Sturgeon under the Endangered Species Act (ESA; 61 FR 4722). Their listing named five Distinct Population Segments (DPSs), predicated on genetic groups composed of geographically proximate populations. Federal management of Atlantic Sturgeon presents challenges, as sturgeon from each of the five DPSs mix extensively in coastal and marine habitats yet take and recovery progress must be evaluated separately for each unit. Genetic assignment testing based on mitochondrial and microsatellite markers allows individuals to be assigned back to their natal river and DPS. However, this approach is not perfect and some individuals may be incorrectly assigned. Recent advances in genomics offer the potential of a higher resolution approach to genetic assignment testing, and thus may reduce uncertainty associated with assignment testing. In addition, genomics allows a greater number of markers to be examined from across a broader portion of the sturgeon genome, thus may provide an enhanced perspective of population structure for the species, and potentially allow other previously intractable questions to be addressed (Bernatchez et al. 2017, Supple and Shapiro 2018). We used next-generation sequencing to develop a draft genome for Atlantic Sturgeon and identify single nucleotide polymorphisms (SNPs) that could be used to resolve the natal river and DPS of individual Atlantic Sturgeon. We identified 1,210 candidate SNPs within the nuclear genome as well as 49 SNPs within the mitochondrial genome. After filtering and review, we selected 161 nuclear SNPs and 39 mitochondrial SNPs for further testing and evaluation. We used genotyping-in-thousands by sequencing (GT-seq) to simultaneously sequence nuclear SNP loci, mitochondrial SNP loci, and the existing panel of twelve microsatellite loci. This effort required a pilot sequencing run on a single sturgeon sample to test marker amplification and refine primer strengths, followed by a series of sequencing runs to generate baseline data for 288 individuals representing nine populations of Atlantic Sturgeon in four DPSs. Using baseline data from the nine populations, we ran a series of genomic analyses to characterize diversity within and among populations, providing a benchmark for this species using the new SNP markers. Allelic richness was similar for all populations, although there was a general trend of more northern population containing greater levels of allelic richness. Interestingly, we observed linkage disequilibrium among many pairs of loci within many populations. This might be the result of physical linkage but could also suggest these populations are recovering from genetic bottlenecks and/or are effectively small, leading to specific haplotypes to be favored by chance. Pairwise differentiation among populations varied among the populations ( F ST range: 0.010-0.098) and was significantly correlated ( r = 0.771; P < 0.001) to pairwise F ST observed using microsatellite markers). Population clustering and ordination techniques using the new genomic data both support an overall population structure that is similar to the current DPS management units (which were developed primarily based on microsatellite genetic data). Overall, this suggests that existing microsatellite markers and the panel of SNP markers developed in this study provide similar information about the populations structure and ecology of Atlantic Sturgeon. Given the observed differences in allele frequencies among populations, our genomic baseline supports previous assertations that Atlantic Sturgeon show natal homing, despite mixing extensively in marine waters during non-breeding periods. Lower levels of differentiation between populations in the South Atlantic DPS suggest that populations in this region may have greater levels of gene flow relative to their more northerly conspecifics, which has also previously been suggested based on microsatellite data. The observed differentiation among populations provides the necessary foundation for determining the natal river and DPS of Atlantic Sturgeon using assignment testing. We tested the utility of our new genomic baseline for resolving the population and DPS of Atlantic Sturgeon. Our nuclear SNP markers showed utility for identifying the origin of unknown Atlantic Sturgeon samples, as 86.5% were assigned to the correct DPS and 66.3% were assigned to the correct natal river. However, since this study was funded the Conservation Genetics and Genomics Laboratory at Leetown Science Center has made significant improvements to their microsatellite genetic baseline, which now performs more effectively than our new genomic approach (the genetic baseline includes 12 populations and 5 DPSs, and correctly assigns 95.8% of individuals to DPS and 84.9% of individuals to their natal population using 12 microsatellite loci). We conducted an ad hoc exploration of how additional microsatellite or nuclear SNP loci may further improve the accuracy of assignment testing. We found that additional microsatellite markers are likely to result in greater improvements in assignment efficiency than additional nuclear SNPs. However, a much larger number of SNP loci (which if identified could be sequenced using other methods that are now available; e.g., the RAD-capture approach published by Ali et al. 2016) could produce assignment efficiencies that are greater than what is currently feasible using microsatellites. In the absence of further research and development of additional SNP markers for Atlantic Sturgeon (possibly using an approach other than GT-seq), the existing microsatellite loci are the most effective means available to determine the natal river and DPS of Atlantic Sturgeon encountered in offshore waters. Because our new genomic markers were less effective than the existing panel of 12 microsatellite markers, we chose to use the existing microsatellite markers to assign Atlantic Sturgeon captured in another BOEM-funded study (cooperative agreement M16AC00003; Monitoring endangered Atlantic Sturgeon and commercial finfish habitat use offshore New York) following consultation with our project officer. Using this approach, we genotyped and assigned 186 Atlantic Sturgeon captured in coastal waters off the Rockaway Peninsula, New York. The vast majority of these sturgeon were assigned to the New York Bight DPS (94.62%), and most appear to belong to the Hudson River population (87.10%) with smaller contributions from the Delaware River population (7.53%). Smaller contributions (2.15%) were observed from six other populations, including those from the James, York, Kennebec, Ogeechee, and Edisto rivers. Although most of the fish we assigned were assigned to the nearest spawning rivers (Hudson and Delaware), the contributions from distant rivers is consistent with the propensity of this species to move long distances and form mixed stock aggregations along the continental shelf. This finding indicates that spawning populations (and their corresponding DPS) from distant locations may potentially be impacted by offshore activities. In fact, activities in this region of the New York Bight could negatively impact Atlantic Sturgeon population from at least four different DPSs. Genetic or genomic assignment testing remains an essential tool to characterize potential impacts to Atlantic Sturgeon populations and should be applied more broadly to better characterize potential impacts of activities in other locations.

Atlantic Coast

VOCs, pesticides, nitrate, and their mixtures in groundwater used for drinking water in the United States

Samples of untreated groundwater from 1255 domestic drinking-water wells and 242 public supply wells were analyzed as part of the National Water-Quality Assessment Program of the U.S. Geological Survey between 1992 and 1999. Wells were sampled to define the regional quality of the groundwater resource and, thus, were distributed geographically across large aquifers, primarily in rural areas. For each sample, as many as 60 volatile organic compounds (VOCs), 83 pesticides, and nitrate were analyzed. On the basis of previous studies, nitrate concentrations as nitrogen &ge;3 mg/L were considered to have an anthropogenic origin. VOCs were detected more frequently (44%) than pesticides (38%) or anthropogenic nitrate (28%). Seventy percent of the samples contained at least one VOC, pesticide, or anthropogenic nitrate; 47% contained at least two compounds; and 33% contained at least three compounds. The combined concentrations of VOCs and pesticides ranged from about 0.001 to 100 &mu;g/L, with a median of 0.02 &mu;g/L. Water from about 12% of the wells contained one or more compounds that exceeded U.S. Environmental Protection Agency drinking-water standards or human health criteria, primarily because of nitrate concentrations exceeding the maximum contaminant level in domestic wells. A mixture is defined as a unique combination of two or more particular compounds, regardless of the presence of other compounds that may occur in the same sample. There were 100 mixtures (significantly associated with agricultural land use) that had a detection frequency between 2% and 19%. There were 302 mixtures (significantly associated with urban land use) that had a detection frequency between 1% and <2%. Only 14 compounds (seven VOCs, six pesticides, and nitrate) contributed over 95% of the detections in these 402 mixtures; however, most samples with these mixtures also contain a variety of other compounds.

Environmental Science & Technology

Analyzing vegetation dynamics of land systems with satellite data

Large area assessment of vegetation conditions is a major requirement for understanding the impact of weather on food, fiber, and forage production. The distribution of vegetation is largely associated with climate, terrain characteristics, and human activity. The interpretation of vegetation dynamics from satellite data can be improved by stratifying the land surface into ecoregions. The Soil Conservation Service, U.S. Department of Agriculture, has developed a system for mapping major land resource areas (MLRA) that groups land areas in the United States on the basis of climate, physiography, land use, and land cover characteristics. In 1989, the U.S. Geological Survey used National Oceanic and Atmospheric Administration weather satellite data to conduct a biweekly assessment of vegetation conditions in 17 western states. Advanced Very High Resolution Radiometer data were acquired daily, and were geographically registered, and the normalized difference vegetation index (NDVI) was computed for the Western United States during the 1989 growing season. Fifteen biweekly NDVI data sets were used to evaluate MLRA's as an appropriate stratification for monitoring and interpreting vegetation conditions in the study area. The results demonstrate the feasibility of using MLRA's to stratify areas for monitoring phenological development and vegetation condition assessment within the growing season. Assessments of the NDVI at biweekly intervals are adequate for monitoring seasonal growth patterns on MLRA's where rangelands, forests, or cultivated agriculture are the primary resource type. Descriptive statistics are indicators of the uniformity or diversity of land use and land cover within an MLRA. Growing season profiles of the NDVI are characterized by the seasonal effects of climate on various land use and land cover classes.

Geocarto International