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Results for “Journal of Visualized Experiments”

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47 records · Page 3Linked to original sources
USGS2023

The smaller, the better? First evaluation of growth and mortality in crayfish internally tagged with p-Chips

Small-bodied aquatic animals present a challenge to researchers seeking to uniquely mark individuals for scientific study. Microtransponder tags, such as p-Chips, represent the smallest electronic animal tags available to meet this need. The use of p-Chips to tag freshwater crayfishes, however, has not been explored. The goal of this study, therefore, was to determine the effects of p-Chip tagging on growth and survival of crayfishes in a controlled laboratory environment. We also investigated potential influences from variables such as sex, reproductive form, number of molt events, and crayfish size on mortality. We internally tagged individuals of the woodland crayfish Faxonius hylas (Faxon, 1890) (12.2–26.6 mm carapace length; CL) with either one p-Chip (single-tag) or one p-Chip and one visual implant elastomer tag (double-tagged) and observed the effects over a period of 90 days. Survival probability over time was not statistically different ( P > 0.05) among tagging groups, sex, and reproductive form. Survival rates were similar across all tagging groups, with 75% in the control group, 77% in the double-tagged group, and 78% in the single-tag group. A strong correlation, however, was observed between survival and the number of molt events. Additionally, there was a negative correlation between survival and crayfish size (CL), indicating a higher risk of mortality for larger individuals. There were no statistically significant differences in growth in CL ( P = 0.30) or mass ( P = 0.19) among the tagging groups. We conclude that p-Chips are a viable tagging option for the study of crayfishes given their size, readability, and retention through molting cycles. We recommend that future studies repeat this experiment using smaller individuals to determine the minimum crayfish size compatible with p-Chip tagging. It is also important to test p-Chips with other crayfish species and compare the growth and mortality of crayfish tagged with p-Chips in natural habitats.

Journal of Crustacean Biology↗
USGS1994

An experiment to detect and locate lightning associated with eruptions of Redoubt Volcano

A commercially-available lightning-detection system was temporarily deployed near Cook Inlet, Alaska in an attempt to remotely monitor volcanogenic lightning associated with eruptions of Redoubt Volcano. The system became operational on February 14, 1990; lightning was detected in 11 and located in 9 of the 13 subsequent eruptions. The lightning was generated by ash clouds rising from pyroclastic density currents produced by collapse of a lava dome emplaced near Redoubt's summit. Lightning discharge (flash) location was controlled by topography, which channeled the density currents, and by wind direction. In individual eruptions, early flashes tended to have a negative polarity (negative charge is lowered to ground) while late flashes tended to have a positive polarity (positive charge is lowered to ground), perhaps because the charge-separation process caused coarse, rapid-settling particles to be negatively charged and fine, slow-settling particles to be positively charged. Results indicate that lightning detection and location is a useful adjunct to seismic volcano monitoring, particularly when poor weather or darkness prevents visual observation. The simultaneity of seismicity and lightning near a volcano provides the virtual certainty that an ash cloud is present. This information is crucial for aircraft safety and to warn threatened communities of impending tephra falls. The Alaska Volcano Observatory has now deployed a permanent lightning-detection network around Cook Inlet.

Journal of Volcanology and Geothermal Research↗
USGS2019

Assessing seasonal changes in microgravity at Yellowstone caldera

Microgravity time series at active volcanoes can provide an indication of mass change related to subsurface magmatic processes, but uncertainty is often introduced by hydrologic variations and other noise sources that cannot easily be isolated. We empirically assessed seasonality and noise by conducting four surveys over the course of May-October 2017 at Yellowstone caldera, Wyoming. Yellowstone experiences frequent changes in the rates and styles of seismicity and surface deformation, but the mechanisms of these changes are poorly understood because the characteristics of the driving fluids are not clear. Past gravity data from the caldera have yielded ambiguous results, possibly due to hydrologic noise. Given the strong visually observable changes in surface water and snow conditions over the course of our surveys, we expected to see significant variations in gravity. The net change in gravity, however, was less than 20 µGal at most sites, and there was no strong correlation with river and lake levels or snow conditions. Seasonal changes in gravity are therefore small compared to those that would be expected from magmatic activity, although they may be on the same order as those associated with Yellowstone’s hydrothermal system. We did find that noise levels in gravity data were highly dependent on site characteristics, with bedrock sites away from trees yielding the lowest levels of noise, and thin concrete pads in forested areas the highest. These results can be used to plan future surveys at Yellowstone and to reinterpret past data, and they provide guidance in terms of best practices for repeat gravity work on volcanoes worldwide.

Wyoming↗
USGS2023

Does hatchery rearing of lake trout affect their reproductive behavior in the wild?

Stocking of hatchery-reared fishes has been used with variable success as a management action to promote the recovery of populations and species. The practice has been controversial for several reasons, including uncertainty about whether the hatchery rearing experience may affect reproduction after release. Fine-scale acoustic telemetry was used during three spawning seasons to test whether hatchery rearing affects the reproductive behavior of lake trout using a spawning shoal complex in northern Lake Huron. Within sex, wild- and hatchery-reared fish behaved similarly, but significant behavioral differences occurred between sexes. Lake trout of both sexes moved synchronously onto the spawning shoals at the completion of autumn thermal turnover and occupied the same spawning sites (confirmed visually by presence of fertilized eggs) on the shoals. Male lake trout tended to congregate directly on spawning sites, with duration of occupancy varying greatly among years. Female lake trout spent less time on spawning shoals than males and congregated less at spawning sites on shoals. Most fish visited multiple spawning sites among shoals per season, with many making multiple transits among individual spawning sites. We found no evidence to support the hypothesis that hatchery rearing impairs spawning behavior of lake trout and, therefore, conclude that behavior deficiencies on the spawning ground are likely not an impediment to rehabilitation of lake trout in northern Lake Huron. Our study narrows the field of possible impediments to lake trout rehabilitation in the Great Lakes and provides insights that expand the conceptual model of lake trout spawning behavior.

Michigan↗
USGS1998

Optical-cell evidence for superheated ice under gas-hydrate-forming conditions

We previously reported indirect but compelling evidence that fine-grained H 2 O ice under elevated CH 4 gas pressure can persist to temperatures well above its ordinary melting point while slowly reacting to form methane clathrate hydrate. This phenomenon has now been visually verified by duplicating these experiments in an optical cell while observing the very slow hydrate-forming process as the reactants were warmed from 250 to 290 K at methane pressures of 23 to 30 MPa. Limited hydrate growth occurred rapidly after initial exposure of the methane gas to the ice grains at temperatures well within the ice subsolidus region. No evidence for continued growth of the hydrate phase was observed until samples were warmed above the equilibrium H 2 O melting curve. With continued heating, no bulk melting of the ice grains or free liquid water was detected anywhere within the optical cell until hydrate dissociation conditions were reached (292 K at 30 MPa), even though full conversion of the ice grains to hydrate requires 6−8 h at temperatures approaching 290 K. In a separate experimental sequence, unreacted portions of H 2 O ice grains that had persisted to temperatures above their ordinary melting point were successfully induced to melt, without dissociating the coexisting hydrate in the sample tube, by reducing the pressure overstep of the equilibrium phase boundary and thereby reducing the rate of hydrate growth at the ice−hydrate interface. Results from similar tests using CO 2 as the hydrate-forming species demonstrated that this superheating effect is not unique to the CH 4 −H 2 O system.

Journal of Physical Chemistry B↗
USGS2022

Evaluation of visual implant elastomer, PIT, and p-Chip tagging methods in a small-bodied minnow species

Reliable fish tagging methods are needed to conduct studies (e.g., movement, habitat, abundance) that inform conservation and management decisions; however, dependable tagging approaches are lacking for many small-bodied fishes. Therefore, our study objective was to determine survival, tag retention (visible implant elastomer [VIE], PIT, or light-activated microtransponder [“p-Chip”]) and tagging effects on short-term growth of the federally threatened Arkansas River Shiner Notropis girardi . We also examined the use of anesthesia to reduce fish mortality (i.e., reduce handling time) and improve tagging efficiency. Fish tagged with p-Chips had the highest survival (87%), whereas VIE-tagged fish had the second highest survival (67%). Visible implant elastomer tags were retained in our fish 88% of the time, and retention was also high using p-Chips (72%). Location of the VIE tag did not significantly affect survival or retention rates. Survival of PIT-tagged fish significantly increased from 53% to 83% when handling time was reduced; however, tag retention was relatively low in both cases (50% and 45%, respectively). The majority of tagged fish across experiments (83–100%) gained weight regardless of tag type. If the research or management goal is to tag Arkansas River Shiner or similar species with individual identification, p-Chips appear to be an appropriate tag. Particularly if cost is limiting or higher tag retention is needed, VIE tags would also be appropriate for batch tagging (i.e., no individual identification). Additional studies to broaden application of tagging to other small-bodied species would be beneficial

Okhahoma↗
USGS2001

Search for life on Mars in surface samples: Lessons from the 1999 Marsokhod rover field experiment

The Marsokhod 1999 field experiment in the Mojave Desert included a simulation of a rover-based sample selection mission. As part of this mission, a test was made of strategies and analytical techniques for identifying past or present life in environments expected to be present on Mars. A combination of visual clues from high-resolution images and the detection of an important biomolecule (chlorophyll) with visible/near-infrared (NIR) spectroscopy led to the successful identification of a rock with evidence of cryptoendolithic organisms. The sample was identified in high-resolution images (3 times the resolution of the Imager for Mars Pathfinder camera) on the basis of a green tinge and textural information suggesting the presence of a thin, partially missing exfoliating layer revealing the organisms. The presence of chlorophyll bands in similar samples was observed in visible/NIR spectra of samples in the field and later confirmed in the laboratory using the same spectrometer. Raman spectroscopy in the laboratory, simulating a remote measurement technique, also detected evidence of carotenoids in samples from the same area. Laboratory analysis confirmed that the subsurface layer of the rock is inhabited by a community of coccoid Chroococcidioposis cyanobacteria. The identification of minerals in the field, including carbonates and serpentine, that are associated with aqueous processes was also demonstrated using the visible/NIR spectrometer. Other lessons learned that are applicable to future rover missions include the benefits of web-based programs for target selection and for daily mission planning and the need for involvement of the science team in optimizing image compression schemes based on the retention of visual signature characteristics.

California↗
USGS2026

Quantifying free-roaming horse body condition: A methodological investigation using game cameras and observer bias corrections

Large populations of free-roaming horses (Equus caballus ) inhabit the western United States, Australia, and other arid and semi-arid rangelands globally. In the United States, horse body condition has been the impetus for gathering and removing horses under an emergency designation and has been noted as a common chronic mortality-causing condition. The Henneke body condition scoring (BCS) system is a qualitative index that includes visual appraisal and physical palpation at multiple anatomical locations on individual horses. This method has been shown to be repeatable between different observers with varying degrees of experience when assessing domestic horses at close proximity. Repeatability using the Henneke BCS system is unknown for free-roaming equids in vast environments, and adequately sampling populations in a minimally biased manner can be logistically challenging. However, estimating body condition using photographs has been shown to be a highly accurate alternative to proximal scoring for multiple scoring systems and species. Our study investigates if an automated, remote camera trap system can be used to estimate free-roaming equid body condition. We compiled existing camera trap data sets from 65 traps into a single data set including more than 3.7 million photos containing images of free-roaming horses from California, Nevada, Utah, and Wyoming. We employed veterinarian-trained animal science students to score horses within randomly sampled, spatially/temporally congruous sequences of animals in our data sets. We compared student scores with veterinarian scores to evaluate whether scorer-specific correction factors are necessary for estimating BCS parameters. Calibration to a fixed reference set reduced between-scorer variance to zero in our population-level BCS estimates. Analysis of our between-scorer variance in our empirical data implies this calibration step can be beneficial when different observers are scoring distinct horse populations across space and/or time.

California, Nevada, Utah, Wyoming↗
USGS2023

The diversity of volcanic hazard maps around the world: Insights from map makers

The IAVCEI Working Group on Hazard Mapping has been active since 2014 and has facilitated several activities to enable sharing of experiences of how volcanic hazard maps are developed and used around the world. One key activity was a global survey of 90 map makers and practitioners to collect data about official, published volcanic hazard maps and how they were developed. The survey asked questions about map content, design, and input data, as well as about the map development process and key lessons learned. Here we present the results of this global survey, which are then used to quantitatively describe and summarise current practices in volcanic hazard map development. We received entries related to 89 volcanic hazard maps (78% long-term/background maps and 22% short-term/crisis hazard maps), covering a total of 80 volcanoes across 28 countries. Although most maps captured in the survey are volcano-scale maps of stratovolcanoes that show similar types of content, such as primary hazard footprints or zones, they vary greatly in input data, communication style, format, appearance, scale, content, and visual design. This diversity stems from a range of factors, including differences in map purpose, the methodology used, the level of understanding of past eruptive history, the prevailing scientific and cartographic practice at the time, the state of volcanic activity, and variations in culture, national map standards and legal requirements. Experiences and lessons shared by our respondents can be divided into six main themes: map design considerations; the process of map development; map audience and map user needs; hazard assessment approach; map availability and accessibility; and external (e.g., political) influences. Insights shared included the importance of: visual design elements, map testing and evaluation, working with stakeholders and end users to improve a map’s efficacy and relevance, and considering possible unanticipated uses of hazard maps. These free-form text insights (i.e., responses to open-ended questions) from map makers and practitioners familiar with the maps lend depth and clarity to our results. They provide a rich complement to our more quantitative analysis of design elements and of approaches used to determine and delineate map zones. Results from our global survey of hazard map makers and practitioners, together with insights from other key initiatives of the Working Group on Hazard Mapping such as the Volcanic Hazard Maps Database (VHMD; https://volcanichazardmaps.org/ ), provide a snapshot of the wide variety of volcanic hazard maps generated over the past decades, and improve our understanding of the diversity across volcanic hazard mapping practices. These initiatives represent important steps towards fulfilling the aims of the Working Group, namely, to construct a framework for a classification scheme for volcanic hazard maps and to promote harmonized terminology, as well as to identify and categorise good practices and considerations for volcanic hazard mapping.

Journal of Applied Volcanology↗
USGS2002

An evaluation of population index and estimation techniques for tadpoles in desert pools

Using visual (VI) and dip net indices (DI) and double-observer (DOE), removal (RE), and neutral red dye capture-recapture (CRE) estimates, we counted, estimated, and censused Couch's spadefoot ( Scaphiopus couchii ) and canyon treefrog ( Hyla arenicolor ) tadpole populations in Big Bend National Park, Texas. Initial dye experiments helped us determine appropriate dye concentrations and exposure times to use in mesocosm and field trials. The mesocosm study revealed higher tadpole detection rates, more accurate population estimates, and lower coefficients of variation among pools compared to those from the field study. In both mesocosm and field studies, CRE was the best method for estimating tadpole populations, followed by DOE and RE. In the field, RE, DI, and VI often underestimated populations in pools with higher tadpole numbers. DI improved with increased sampling. Larger pools supported larger tadpole populations, and tadpole detection rates in general decreased with increasing pool volume and surface area. Hence, pool size influenced bias in tadpole sampling. Across all techniques, tadpole detection rates differed among pools, indicating that sampling bias was inherent and techniques did not consistently sample the same proportion of tadpoles in each pool. Estimating bias (i.e., calculating detection rates) therefore was essential in assessing tadpole abundance. Unlike VI and DOE, DI, RE, and CRE could be used in turbid waters in which tadpoles are not visible. The tadpole population estimates we used accommodated differences in detection probabilities in simple desert pool environments but may not work in more complex habitats.

Journal of Herpetology↗
USGS2012

Laboratory studies of volcanic jets

The study of the fluid dynamics of violent volcanic eruptions by laboratory experiment is described, and the important fluid-dynamic processes that can be examined in laboratory models are discussed in detail. In preliminary experiments, pure gases are erupted from small reservoirs. The gases used are Freon 12 and Freon 22, two gases of high molecular weight and high density that are good analogs of heavy and particulate-laden volcanic gases; nitrogen, a moderate molecular weight, moderate density gas for which the thermodynamic properties are well known; and helium, a low molecular weight, lowdensity gas that is used as a basis for comparison with the behavior of the heavier gases and as an analog of steam, the gas that dominates many volcanic eruptions. Transient jets erupt from the reservoir into the laboratory upon rupture of a thin diaphragm at the exit of a convergent nozzle. The gas accelerates from rest in the reservoir to high velocity in the jet. Reservoir pressures and geometries are such that the fluid velocity in the jets is initially supersonic and later decays to subsonic. The measured reservoir pressure decreases as the fluid expands through repetitively reflecting rarefaction waves, but for the conditions of these experiments, a simple steady-discharge model is sufficient to explain the pressure decay and to predict the duration of the flow. Density variations in the flow field have been visualized with schlieren and shadowgraph photography. The observed structure of the jet is correlated with the measured pressure history. The starting vortex generated when the diaphragm ruptures becomes the head of the jet. Though the exit velocity is sonic, the flow head in the helium jet decelerates to about one-third of sonic velocity in the first few nozzle diameters, the nitrogen head decelerates to about three-fourths of sonic velocity, while Freon maintains nearly sonic velocity. The impulsive acceleration of reservoir fluid into the surrounding atmosphere produces a compression wave. The strength of this wave depends primarily on the sound speed of the fluid in the reservoir but also, secondarily with opposite effect, on the density: helium produces a relatively strong atmospheric shock while the Freons do not produce any optically observable wave front. Well-formed N waves are detected with a microphone far from the reservoir. Barrel shocks, Mach disks, and other familiar features of steady underexpanded supersonic jets form inside the jet almost immediately after passage of the flow head. These features are maintained until the pressure in the reservoir decays to sonic conditions. At low pressures the jets are relatively structureless. Gas-particle jets from volcanic eruptions may behave as pseudogases if particle concentrations and mass and momentum exchange between the components are sufficiently small. The sound speed of volcanic pseudogases can be as large as 1000 m s −1 or as small as a few tens of meters per second depending on the mass loading and initial temperature. Fluids of high sound speed produce stronger atmospheric shock waves than do those of low sound speed. Therefore eruption of a hot gas lightly laden with particulates should produce a stronger shock than eruption of a cooler or heavily laden fluid. An empirical expression suggests that the initial velocity of the head of supersonic volcanic jets is controlled by the sound speed and the ratio of the density of the erupting fluid to that of the atmosphere. The duration of gas or pseudogas eruptions is controlled by the sound speed of the fluid and the ratio of reservoir volume to vent area.

Journal of Geophysical Research Solid Earth↗