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At least 55 records · Page 3Linked to original sources

Life history, genetics, range expansion and new frontiers of the lionfish (Pterois volitans, Perciformes: Pteroidae) in Latin America

Pterois volitans (lionfish) is a midsize predatory fish commonly found in waters of the western Pacific and Indian Ocean. The species was first documented in Dania Beach, Florida (northwestern Caribbean) in 1985. Since that time the species has expanded its range rapidly to the Northwestern Atlantic Ocean, Gulf of Mexico, and Caribbean Sea. Since its introduction P. volitans has changed community structure and biodiversity of Caribbean reef communities and other coastal tropical ecosystems. Continuous introductions (accidental or intentional), limited natural predators, naïve-range prey behavior, high predation rates on competitors, continuous reproduction, and an extended period of larval dispersal have been the keys for successful invasion and rapid range extension of P. volitans . This invasion has become so severe that it has been recognized as one of the world’s top conservation issues. Here, we review the life history, behavior, and historical and contemporary genetic patterns that facilitate expansion and the colonization process. A greater understanding of lionfish biology, ecology, and the changes related to its present condition as a super-invader could improve current and future management strategies and new detection and response methodologies. We also examine new invasion frontiers that this species has the potential to colonize such as the eastern Pacific. This information will provide managers, the scientific community, and the civil society better tools for eradication, control and management of future invasions of this and other invasive species.

Caribbean Sea, Latin America↗

Watering the forest for the trees: An emerging priority for managing water in forest landscapes

Widespread threats to forests resulting from drought stress are prompting a re-evaluation of priorities for water management on forest lands. In contrast to the widely held view that forest management should emphasize providing water for downstream uses, we argue that maintaining forest health in the context of a changing climate may require focusing on the forests themselves and on strategies to reduce their vulnerability to increasing water stress. Management strategies would need to be tailored to specific landscapes but could include thinning, planting and selecting for drought-tolerant species, irrigating, and making more water available to plants for transpiration. Hydrologic modeling reveals that specific management actions could reduce tree mortality due to drought stress. Adopting water conservation for vegetation as a priority for managing water on forested lands would represent a fundamental change in perspective and potentially involve trade-offs with other downstream uses of water.

Frontiers in Ecology and the Environment↗

Stony coral tissue loss disease in Florida is associated with disruption of host–zooxanthellae physiology

Samples from eight species of corals ( Colpophyllia natans , Dendrogyra cylindrus , Diploria labyrinthiformis , Meandrina meandrites , Montastraea cavernosa , Orbicella faveolata, Pseudodiploria strigosa , and Siderastrea siderea ) that exhibited gross clinical signs of acute, subacute, or chronic tissue loss attributed to stony coral tissue loss disease (SCTLD) were collected from the Florida Reef Tract during 2016–2018 and examined histopathologically. The hallmark microscopic lesion seen in all eight species was focal to multifocal lytic necrosis (LN) originating in the gastrodermis of the basal body wall (BBW) and extending to the calicodermis, with more advanced lesions involving the surface body wall. This was accompanied by other degenerative changes in host cells such as mucocyte hypertrophy, degradation and fragmentation of gastrodermal architecture, and disintegration of the mesoglea. Zooxanthellae manifested various changes including necrosis (cytoplasmic hypereosinophilia, pyknosis); peripheral nuclear chromatin condensation; cytoplasmic vacuolation accompanied by deformation, swelling, or atrophy; swollen accumulation bodies; prominent pyrenoids; and degraded chloroplasts. Polyhedral intracytoplasmic eosinophilic periodic acid–Schiff-positive crystalline inclusion bodies (∼1–10 μm in length) were seen only in M. cavernosa and P. strigosa BBW gastrodermis in or adjacent to active lesions and some unaffected areas (without surface lesions) of diseased colonies. Coccoidlike or coccobacilloidlike structures (Gram-neutral) reminiscent of microorganisms were occasionally associated with LN lesions or seen in apparently healthy tissue of diseased colonies along with various parasites and other bacteria all considered likely secondary colonizers. Of the 82 samples showing gross lesions of SCTLD, 71 (87%) were confirmed histologically to have LN. Collectively, pathology indicates that SCTLD is the result of a disruption of host–symbiont physiology with lesions originating in the BBW leading to detachment and sloughing of tissues from the skeleton. Future investigations could focus on identifying the cause and pathogenesis of this process.

Florida↗

Pacific summary report 2: A revision of Outer Continental Shelf oil and gas activities in the Pacific (Southern California) and their onshore impacts: A summary report, May 1980

The Pacific Outer Continental Shelf (OCS) is an established hydrocarbon-producing region. Oil and gas have been produced from the Santa Barbara Channel (both State and Federal acreage) since 1896. Almost 77,000 barrels of oil are produced from the California Federal OCS each day as of December 1981, and leases on State tidelands produce about 40,000 barrels of oil per day. This highly developed area is, of course, but a small part of the Pacific OCS, which also includes Northern California, Washington, and Oregon. The petroleum industry has expressed interest in exploring frontier areas, and as frontier acreage is offered in future lease sales, exploration, development, and production may move out into deeper water. The technology is currently being developed and tested to allow deepwater exploration. To date, offshore drilling operations have resulted in the delineation of 11 offshore oil and gas fields and 2 gas fields in the Southern California OCS. A recent discovery off Point Arguello, California, may lead to delineation of a new field. Reserve estimates have been made for the known fields in the region. Remaining recoverable reserves are estimated at 787 million barrels of oil and over 1.7 trillion cubic feet of gas as of December 31, 1980. Estimates of undiscovered recoverable resources in the offshore Santa Maria Basin and Southern California OCS are over 3.5 billion barrels of oil and in excess of 5 trillion cubic feet of gas. To date, there have been seven oil and gas lease sales in the Pacific OCS Region. An average of one lease sale per year is scheduled through 1985. Industry interest indicates that the Santa Barbara Channel's potential has not yet been fully explored, and some basins to the south, as well as in the Santa Maria Basin, are now gaining considerable interest. Future exploratory activity in the San Pedro Bay and the Santa Maria Basin is likely to increase. Exploration, development, and production in the Pacific OCS Region are increasing at a moderate pace. Pipelines continue to be the preferred mode of transporting Pacific OCS hydrocarbons to shore. The State and the Federal Governments have expressed a commitment to the use of pipelines where possible and economically feasible. Tankers traveling in the Pacific and entering California ports carry, for the most part, imported oil. The region is active in OCS transportation planning; the Bureau of Land Management works through the Pacific States Regional Technical Working Group Committee. Onshore, the Petroleum Transportation Committee (formerly the Joint Government/Industry Pipeline Working Group) is evaluating a number of transportation scenarios. The nearshore and onshore petroleum processing facilities in the Pacific Region service onshore and offshore oil and gas exploration, development, and production, as well as the international oil market. Many of the onshore facilities have been in operation for years, supporting California's extensive onshore and offshore production. Several new facilities have been proposed, are currently under construction, or have recently opened. Shell Oil opened a crude oil distribution facility near Long Beach in December 1980. Operations began at Union Oil's Mandalay Beach separation and treatment plant in December 1981. The Pacific Offshore Pipeline Company, a subsidiary of the Pacific Lighting Corporation, is planning to build a gas treatment plant at Las Flores Canyon; completion of this project is scheduled for July 1983. It is expected that these new facilities, in addition to the established plants, will be able to accommodate any new OCS production in the near term from previously leased areas.

California↗

Water resources of Hot Springs County, Wyoming

The wells and springs inventoried in Hot Springs County most commonly had been completed in or issued from the Quaternary alluvium, Quaternary terrace deposits, Fort Union and Mesaverde Formations, Cody Shale, and the Frontier and Chugwater Formations. The largest discharges measured were from the Quaternary terrace deposits (400 gallons per minute) and the Phosphoria Formation (1,000 gallons per minute). Discharges from all other geologic units varied, but most wells and springs yielded 50 gallons per minute or less. Water-quality samples collected from springs that issued from the Absaroka Volcanic Supergroup, the Bighorn Dolomite, and the Flathead Sandstone had the lowest dissolved-solids concentrations, which ranged from 58 to 265 milligrams per liter, and the least variable water types. Water from the volcanic rocks was a sodium bicarbonate type; whereas, water from the Flathead Sandstone was a calcium bicarbonate type. Water types for all the other aquifers varied from sampling site to sampling site; however, water samples from the Fort Union Formation and the Cody Shale were consistently of the sodium sulfate type. The effect of oil- and gas-development at Hamilton Dome on thermal spring discharges at Hot Springs State Park near Thermopolis was studied. The estimated drawdown from 1918, when the Hamilton Dome oil field was discovered, to 1988 was made using drill-stem data from previous studies. Drawdown at Big Spring in the Park was estimated to be less than 3 feet on the basis of recent oil- and water-production data, previous modeling studies, and the estimated water-level drawdown of 330 feet in wells at the Hamilton Dome oil field. Streams originating in the Plains region of the county, such as Middle Fork Owl Creek, are ephemeral or intermittent; whereas, streams originating in the mountains, such as Gooseberry Creek, are perennial. Average annual runoff across the county ranges from 0.26 inches at a representative streamflow-gaging station near Worland in the plains region to 5.4 inches in the Owl Creek Mountains and southeastern Absaroka Range.

Wyoming↗

Selected crater and small caldera lakes in Alaska: Characteristics and hazards

This study addresses the characteristics, potential hazards, and both eruptive and non-eruptive role of water at selected volcanic crater lakes in Alaska. Crater lakes are an important feature of some stratovolcanoes in Alaska. Of the volcanoes in the state with known Holocene eruptive activity, about one third have summit crater lakes. Also included are two volcanoes with small caldera lakes (Katmai, Kaguyak). The lakes play an important but not well studied role in influencing eruptive behavior and pose some significant hydrologic hazards. Floods from crater lakes in Alaska are evaluated by estimating maximum potential crater lake water volumes and peak outflow discharge with a dam-break model. Some recent eruptions and hydrologic events that involved crater lakes also are reviewed. The large volumes of water potentially hosted by crater lakes in Alaska indicate that significant flowage hazards resulting from catastrophic breaching of crater rims are possible. Estimates of maximum peak flood discharge associated with breaching of lake-filled craters derived from dam-break modeling indicate that flood magnitudes could be as large as 10 3 –10 6 m 3 /s if summit crater lakes drain rapidly when at maximum volume. Many of the Alaska crater lakes discussed are situated in hydrothermally altered craters characterized by complex assemblages of stratified unconsolidated volcaniclastic deposits, in a region known for large magnitude (>M7) earthquakes. Although there are only a few historical examples of eruptions involving crater lakes in Alaska, these provide noteworthy examples of the role of external water in cooling pyroclastic deposits, acidic crater-lake drainage, and water-related hazards such as lahars and base surge.

Alaska↗

Heavy-Mineral Placer Potential Map of the U.S. Continental Shelf, Western and Northern Gulf of Mexico

The establishment of the Exclusive Economic Zone (EEZ) in 1983 by Presidential Proclamation opened for natural resource exploration a vast offshore frontier area contiguous to the United States and its territories. The EEZ extends from the seaward limit of state waters (3 nautical mi from shore) to 200 nautical mi offshore, and it includes the continental shelves. Within the context of the EEZ natural resource assessment effort, the purpose of this study is to delineate, on a regional basis, the potential for heavy-mineral placers on the U.S. Continental Shelf in the western and northern Gulf of Mexico from the United States-Mexico border to the Alabama-Florida state line. This map is intended to serve as a general guide for placer exploration. It shows favorable sea-floor areas for placer occurrence in water depths ranging from 0 to 100 fathoms (600 ft). The map can be used as a guide for focusing costly exploratory efforts, such as coring operations and geophysical surveys. The potential economic value of heavy-mineral placer concentrations on the U.S. Continental Shelf is a function of both geologic and economic variables. Geologic variables include the composition and concentration of the heavy-mineral assemblages and their environment of deposition. Economic variables include the current world market price of extracted metals, as well as the cost of mining, processing, and marketing the metals. These economic factors, in turn, are tempered by the nation1s socio-political climate, which determines its need for specific mineral resources at any given time.

Miscellaneous Field Studies Map↗

What’s It worth? Estimating the potential value of early warnings of cyanobacterial harmful algal blooms for managing freshwater reservoirs in Kansas, United States

Cyanobacterial blooms are an issue drawing increasing concern in freshwater lakes and reservoirs in the United States due to the real and sometimes perceived harms they can cause through cyanotoxin production or other effects. These types of blooms are often referred to as cyanobacterial harmful algal blooms (cyanoHABs). Cyanotoxin exposure can potentially lead to human health effects through recreation and consumption of drinking water and may impact fisheries, wildlife, domestic pets, and livestock. Characterizing the societal impacts of cyanotoxin production, exposure, and effects and estimating the potential value of information of an early warning system can inform and support freshwater lake and reservoir management decisions and future research directions. A Bayesian decision tree analysis was utilized to identify uses, users, and benefits of the information provided by this research. Specifically, the potential value related to a cyanoHAB early warning system, based on potential toxicity, was analyzed that would provide information two additional days earlier relative to cyanoHAB toxicity. The evaluation considers the application of this information for freshwater lake management - whether or not to post an advisory or warning to avoid recreational water contact. The model was parameterized with data from the state of Kansas and the value of avoided foregone recreation and avoided health effects was derived. The estimated annual value of information ranges between \$565 thousand to \$2.3 million (2018 United States Dollars (USD)) for the state of Kansas alone based on provided assumptions. The results demonstrate a lower bound of the value of a cyanoHAB early warning system and suggest additional research to understand how the use and value of this information could support research prioritization and further illustrate the return on research investment. This analysis does not incorporate the full suite of potential societal costs that may be associated with a cyanoHAB event such as drinking water treatment, impacts to irrigation, or power generation.

Kansas↗

Salmon, forage fish, and kelp

Kelp beds are prominent features of northeast Pacific coastlines. They are seasonal in nature, as are the communities that use them. Here, juvenile and adult Chinook salmon ( Oncorhynchus tshawytscha ) – key components of northeast Pacific marine food webs that link plankton and forage fishes to endangered killer whales – have just arrived at the coastal kelp beds (left) and are feeding on the large schools of forage fish such as young‐of‐the‐year herring, which are also migrating in great numbers near the shore. Juvenile herring and smelt will soon move offshore to grow and feed, and finally return as adults to spawn along shorelines. Rapid growth is critical to the survival of young salmon. They quickly learn to work together to herd the small, skittish prey into tight groups. The kelp beds play an important role for both the salmon and their prey, providing refuge for feeding salmon and enhanced prey resources for hungry forage fish, which in turn feed incessantly at the surface of the kelp beds, except when they are disrupted by lightning‐fast attacks by marauding salmon. By October, much of the kelp will be gone, as will the juvenile salmon and forage fish, replaced by their adult congeners (right) that have traveled for years and hundreds of miles to continue the cycle. Globally, kelp forests are in flux. Disturbances, including those induced by climate change, may have serious implications not only for this critical nearshore phase of salmon and forage fish, but also for the future viability of our cold‐water northeast Pacific marine ecosystems.

Frontiers in Ecology and the Environment↗

Dynamics of green and blue water supply stress index across major global cropland basins

Global food and water insecurity could be serious problems in the upcoming decades with growing demands from the increasing global population and more frequent effect of climatic extremes. As the available water resources are diminishing and facing continuous stress, it is crucial to monitor water demand and water availability to understand the associated water stresses. This study assessed the water stress by applying the water supply stress index (WaSSI) in relation to green (WaSSI G ) and blue (WaSSI B ) water resources across six major cropland basins including the Mississippi (North America), San Francisco (South America), Nile (Africa), Danube (Europe), Ganges-Brahmaputra (Asia), and Murray-Darling (Australia) for the past 17-years (2003–2019). The WaSSI G and WaSSI B results indicated that the Murray-Darling Basin experienced the most severe (maximum WaSSI G and WaSSI B anomalies) green and blue water stresses and the Mississippi Basin had the least. All basins had both green and blue water stresses for at least 35% (6 out of 17 years) of the study period. The interannual variations in green water stress were driven by both crop water demand and green water supply, whereas the blue water stress variations were primarily driven by blue water supply. The WaSSI G and WaSSI B provided a better understanding of water stress (blue or green) and their drivers (demand or supply driven) across cropland basins. This information can be useful for basin-specific resource mobilization and interventions to ensure food and water security.

Frontiers in Climate↗

The mysterious case of the missing razor clams

Oceans are changing and these changes are affecting animals that live there. Animals respond differently to changes in water temperature, food availability, or contaminants. Those responses can be seen in their genes. Gene transcription is a tool that allows scientists to see the response of an animal’s genes to its environment. We used gene transcription to compare two populations of Pacific razor clams in Alaska: one that has lots of clams and one that used to have lots but doesn’t anymore. We were surprised when we didn't find any differences in their gene responses! So, we had to think about what else might be influencing the number of clams in these two populations. As we “dug” for answers, we found out that there are differences between the populations that don‘t influence their genes but may impact their numbers, such as predation.

Frontiers for Young Minds↗

The Mid-Cretaceous Frontier Formation near the Moxa Arch, southwestern Wyoming

The Frontier Formation in the Green River Basin of Wyoming, Utah, and Colorado, consists of sandstone, siltstone, and shale, and minor conglomerate, coal, and bentonite. These strata were deposited in several marine and nonmarine environments during early Late Cretaceous time. At north-trending outcrops along the eastern edge of the overthrust belt, the Frontier is of Cenomanian, Turonian, and early Coniacian age, and commonly is about 610 m (2,000 ft) thick. The formation in that area conformably overlies the Lower Cretaceous Aspen Shale and is divided into the following members, in ascending order: Chalk Creek, Coalville, Allen Hollow, Oyster Ridge Sandstone, and Dry Hollow. In west-trending outcrops on the northern flank of the Uinta Mountains in Utah, the Frontier is middle and late Turonian, and is about 60 m (200 ft) thick. These strata disconformably overlie the Lower Cretaceous Mowry Shale. In boreholes on the Moxa arch, the upper part of the Frontier is of middle Turonian to early Coniacian age and unconformably overlies the lower part of the formation, which is early Cenomanian at the south end and probably Cenomanian to early Turonian at the north end. The Frontier on the arch thickens northward from less than 100 m (328 ft) to more than 300 m (984 ft) and conformably overlies the Mowry. The marine and nonmarine Frontier near the Uinta Mountains, marine and mnmarine beds in the upper part of the formation on the Moxa arch and the largely nonmarine Dry Hollow Member at the top of the Frontier in the overthrust belt are similar in age. Older strata in the formation, which are represented by the disconformable basal contact of the Frontier near the Uinta Mountains, thicken northward along the Moxa arch and westward between the arch and the overthrust belt. The large changes in thickness of the Frontier in the Green River Basin were caused mainly by differential uplift and truncation of the lower part of the formation during the early to middle Turonian and by the shoreward addition of progressively younger sandstone units at the top of the formation during the late Turonian and early Coniacian. The sandstone in cores of the Frontier, from boreholes on the Moxa arch and the northern plunge of the Rock Springs uplift, consists of very fine grained and fine-grained litharenites and sublitharenites that were deposited in deltaic and shallow-water marine environments. These rocks consist mainly of quartz, chert, rock fragments, mixed-layer illite-smectite, mica-illite, and chlorite. Samples of the sandstone have porosities of 4.7 to 23.0 percent and permeabilities of 0.14 to 6.80 millidarcies, and seem to represent poor to fair reservoir beds for oil and gas. The shale in cores of the Frontier Formation and the overlying basal Hilliard Shale, from the Moxa arch, Rock Springs uplift, and overthrust belt, was deposited in deltaic and offshore-marine environments. Samples of the shale are composed largely of quartz, micaillite, mixed-layer illite-smectite, kaolin, and chlorite. They also contain from 0.27 to 4.42 percent organic carbon, in humic and sapropelic organic matter. Most of the sampled shale units are thermally mature, in terms of oil generation, and a few probably are source rocks for oil and gas.

Professional Paper↗

Land development and road salt usage drive long-term changes in major-ion chemistry of streamwater in six exurban and suburban watersheds, southeastern Pennsylvania, 1999-2019

In urbanized areas, the “freshwater salinization syndrome” (FSS), which pertains to long-term increases in concentrations of major ions and metals in fresh surface waters, has been attributed to road salt application. In addition to FSS, the water composition changes as an influx of sodium (Na + ) in recharge may displace calcium (Ca 2+ ), magnesium (Mg 2+ ), potassium (K + ), and trace metals by reverse cation exchange. These changing ion fluxes can result in adverse impacts on groundwater and surface waters used for municipal supplies. Few datasets exist to quantify the FSS on a watershed scale or link its manifestation to potential controlling factors such as changes in urban development, land use/land cover (LULC), or wastewater treatment plant (WWTP) discharges in upstream areas. Here, we use two decades (1999–2019) of monthly streamwater quality data combined with daily streamflow for six exurban and suburban watersheds in southeastern Pennsylvania to examine the relations among Ca 2+ , Mg 2+ , K + , Na + , chloride (Cl − ), sulfate (SO 4 2- ), and alkalinity (HCO 3 − ) concentrations and upstream controlling factors. Flow-normalized annual and baseflow (August ̶ November) concentrations for Ca 2+ , Mg 2+ , Na + , and Cl − increased in all six watersheds over the 20-year study, providing evidence of FSS’s impacts on groundwater that sustains streamflow. Additionally, a redundancy analysis using 2019 flow-normalized values identified the following positive associations between solute concentrations and controlling variables: 1) Cl − , Mg 2+ , and Ca 2+ with impervious surface cover (ISC), 2) Na + and SO 4 2- with ISC and total WWTP discharge volume, and 3) HCO 3 − with agriculture and total WWTP discharge volume. From a human health perspective, 2019 flow-normalized Na + concentrations exceeded the U.S. Environmental Protection Agency’s 20 mg L -1 threshold for individuals restricted to a low sodium diet. Furthermore, indices used to evaluate the corrosivity of source waters to drinking water infrastructure and inform municipal water treatment practices, such as the Chloride to Sulfate Mass Ratio and Larson Ratio, increased between two- and seven-fold over the 20-year time. Collectively, the results elucidate the causal factors of the FSS in suburban and exurban watersheds and its potential impacts on human health and drinking water infrastructure.

Pennsylvania↗

Proceedings of the third USGS modeling conference, June 7-11, 2010, Broomfield, Colorado-Understanding and predicting for a changing world

The Third USGS Modeling Conference was held June 7th-11, 2010, in Broomfield, Colorado. The conference focused on the development and application of analytical and theoretical models and data availability that support managing the Nation's resources and help protect lives and property. Participants at the conference included scientists and managers from Department of the Interior (DOI) Bureaus; national and international Federal, State, and local agencies; academic institutions; and nongovernmental organizations. The conference was organized according to DOI priorities and the strategic directions of the USGS Science Strategy; the following themes were emphasized: (1) Understanding Ecosystems and Restoring America's Treasured Landscapes; (2) Climate Change and Impact; (3) New Energy Frontier and Minerals for America; (4) A National Hazards, Risk, and Resilience Assessment Program; (5) Role of Environment and Wildlife in Human Health; (6) A Water Census of the United States; and (7) New Methods of Investigation and Discovery. The conference theme-"Understanding and Predicting for a Changing World"-focused on the following goals: advance development and application of models; provide tools that address management issues; present state-of-the-art models ranging from individual phenomena to integrated systems; and foster a working community among scientists and managers.

Scientific Investigations Report↗

Alaska Arctic marine fish ecology catalog

The marine fishes in waters of the United States north of the Bering Strait have received new and increased scientific attention over the past decade (2005–15) in conjunction with frontier qualities of the region and societal concerns about the effects of Arctic climate change. Commercial fisheries are negligible in the Chukchi and Beaufort Seas, but many marine species have important traditional and cultural values to Alaska Native residents. Although baseline conditions are rapidly changing, effective decisions about research and monitoring investments must be based on reliable information and plausible future scenarios. For the first time, this synthesis presents a comprehensive evaluation of the marine fish fauna from both seas in a single reference. Although many unknowns and uncertainties remain in the scientific understanding, information presented here is foundational with respect to understanding marine ecosystems and addressing dual missions of the U.S. Department of the Interior for energy development and resource conservation.

Scientific Investigations Report↗

Influence of filter pore size on composition and relative abundance of bacterial communities and select host-specific MST markers in coastal waters of southern Lake Michigan

Water clarity is often the primary guiding factor in determining whether a prefiltration step is needed to increase volumes processed for a range of microbial endpoints. In this study, we evaluate the effect of filter pore size on the bacterial communities detected by 16S rRNA gene sequencing and incidence of two host-specific microbial source tracking (MST) markers in a range of coastal waters from southern Lake Michigan, using two independent data sets collected in 2015 (bacterial communities) and 2016–2017 (MST markers). Water samples were collected from river, shoreline, and offshore areas. For bacterial communities, each sample was filtered through a 5.0-μm filter, followed by filtration through a 0.22-μm filter, resulting in 70 and 143 filter pairs for bacterial communities and MST markers, respectively. Following DNA extraction, the bacterial communities were compared using 16S rRNA gene amplicons of the V3–V4 region sequenced on a MiSeq Illumina platform. Presence of human ( Bacteroides HF183) and gull (Gull2, Catellicoccus marimammalium ) host-specific MST markers were detected by qPCR. Actinobacteriota, Bacteroidota, and Proteobacteria, collectively represented 96.9% and 93.9% of the relative proportion of all phyla in the 0.22- and 5.0-μm pore size filters, respectively. There were more families detected in the 5.0-μm pore size filter (368) than the 0.22-μm (228). There were significant differences in the number of taxa between the two filter sizes at all levels of taxonomic classification according to linear discriminant analysis (LDA) effect size (LEfSe) with as many as 986 taxa from both filter sizes at LDA effect sizes greater than 2.0. Overall, the Gull2 marker was found in higher abundance on the 5.0-μm filter than 0.22 μm with the reverse pattern for the HF183 marker. This discrepancy could lead to problems with identifying microbial sources of contamination. Collectively, these results highlight the importance of analyzing pre- and final filters for a wide range of microbial endpoints, including host-specific MST markers routinely used in water quality monitoring programs. Analysis of both filters may increase costs but provides more complete genomic data via increased sample volume for characterizing microbial communities in coastal waters.

Illinois, Indiana, Wisconsin↗

Machine learning for understanding inland water quantity, quality, and ecology

This chapter provides an overview of machine learning models and their applications to the science of inland waters. Such models serve a wide range of purposes for science and management: predicting water quality, quantity, or ecological dynamics across space, time, or hypothetical scenarios; vetting and distilling raw data for further modeling or analysis; generating and exploring hypotheses; estimating physically or biologically meaningful parameters for use in further modeling; and revealing patterns in complex, multidimensional data or model outputs. An important research frontier is the injection of limnological knowledge into machine-learning models, which has shown great promise for increasing such models’ accuracy, trustworthiness, and interpretability. Here we describe a few of the most powerful machine learning tools, describe best practices for employing these tools and injecting knowledge guidance, and give examples of their applications to advance understanding of inland waters.

Book chapter↗