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Percentile distributions of median nitrite plus nitrate as nitrogen, total nitrogen, and total phosphorus concentrations in Oklahoma streams, 1973-2001

Nutrients are one of the primary causes of water-quality impairments in streams, lakes, reservoirs, and estuaries in the United States. The U.S. Environmental Protection Agency has developed regional-based nutrient criteria using ecoregions to protect streams in the United States from impairment. However, nutrient criteria were based on nutrient concentrations measured in large aggregated nutrient ecoregions with little relevance to local environmental conditions in states. The Oklahoma Water Resources Board is using a dichotomous process known as Use Support Assessment Protocols to define nutrient criteria in Oklahoma streams. The Oklahoma Water Resources Board is modifying the Use Support Assessment Protocols to reflect nutrient informa-tion and environmental characteristics relevant to Oklahoma streams, while considering nutrient information grouped by geographic regions based on level III ecoregions and state boundaries. Percentile distributions of median nitrite plus nitrate as nitrogen, total nitrogen, and total phosphorous concentrations were calculated from 563 sites in Oklahoma and 4 sites in Arkansas near the Oklahoma and Arkansas border to facilitate development of nutrient criteria for Oklahoma streams. Sites were grouped into four geographic regions and were categorized into eight stream categories by stream slope and stream order. The 50th percentiles of median nitrite plus nitrate as nitrogen, total nitrogen, and total phosphorus concentrations were greater in the Ozark Highland ecoregion and were less in the Ouachita Mountains ecoregion when compared to other geographic areas used to group sites. The 50th percentiles of median concentrations of nitrite plus nitrate as nitrogen, total nitrogen, and total phosphorus were least in first, second, and third order streams. The 50th percentiles of median nitrite plus nitrate as nitrogen, total nitrogen and total phosphorus concentrations in the Ozark Highland and Ouachita Mountains ecoregions were least in first, second, and third order streams with streams slopes greater than 17 feet per mile. Nitrite plus nitrate as nitrogen and total nitrogen criteria determined by the U.S. Environmental Protection Agency for the Ozark Highland ecoregion were less than the 25th percentiles of median nitrite plus nitrate as nitrogen, total nitrogen, and total phosphorus concentrations in the Ozark Highland ecoregion calculated for this report. Nitrite plus nitrate as nitrogen and total nitrogen criteria developed by the U.S. Environmental Protection Agency for the Ouachita Mountains ecoregion were similar to the 25th percentiles of median nitrite plus nitrate as nitrogen and total nitrogen concentrations in the Ouachita Mountains ecoregion calculated for this report. Nitrate as nitrogen and total phosphorus concentrations currently (2002) used in the Use Support Assessment Protocols for Oklahoma were greater than the 75th percentiles of median nitrite plus nitrate as nitrogen and total phosphorus concentrations calculated for this report.

Oklahoma

Monitoring wadeable stream habitat conditions in Southeast Coast Network parks: Protocol narrative

The Southeast Coast Network (SECN) has initiated a monitoring effort to assess habitat conditions in wadeable streams at national parks, recreation areas, battlefields, and monuments in Alabama, Georgia, and South Carolina. This monitoring effort includes Chattahoochee River National Recreation Area, Kennesaw Mountain National Battlefield Park, Congaree National Park, Horseshoe Bend National Military Park, and Ocmulgee National Monument. Stream habitat monitoring was implemented in 2016, and focuses specifically on providing relevant data to assess the physical condition of Piedmont and upper Coastal Plain streams with respect to aquatic and riparian habitats and how these habitats may be changing over time. The habitat assessment methods proposed in this protocol rely on standard data collection methods and standard operating procedures currently in use by the U.S. Geological Survey, U.S. Environmental Protection Agency, and U.S. Forest Service that have been modified to better meet the needs of National Park Service (NPS) managers. The Southeast Coast Network’s wadeable stream protocol was developed to begin a monitoring program that will provide insight into the status of, and trends in, stream and riparian habitat conditions. The number of reaches surveyed at each park is dependent on the spatial extent of the park and the total number of wadeable streams that are present within park boundaries. Regardless of the size of the park and the number of reaches that are to be monitored, selected reaches (1) are representative of the processes influencing the streams in each park; (2) can address current and anticipated management concerns, and (3) offer the most utility for future complementary studies.

Natural Resource Report

Remote sensing for grassland management in the arid Southwest

We surveyed a group of rangeland managers in the Southwest about vegetation monitoring needs on grassland. Based on their responses, the objective of the RANGES (Rangeland Analysis Utilizing Geospatial Information Science) project was defined to be the accurate conversion of remotely sensed data (satellite imagery) to quantitative estimates of total (green and senescent) standing cover and biomass on grasslands and semidesert grasslands. Although remote sensing has been used to estimate green vegetation cover, in arid grasslands herbaceous vegetation is senescent much of the year and is not detected by current remote sensing techniques. We developed a ground truth protocol compatible with both range management requirements and Landsat's 30 m resolution imagery. The resulting ground-truth data were then used to develop image processing algorithms that quantified total herbaceous vegetation cover, height, and biomass. Cover was calculated based on a newly developed Soil Adjusted Total Vegetation Index (SATVI), and height and biomass were estimated based on reflectance in the near infrared (NIR) band. Comparison of the remotely sensed estimates with independent ground measurements produced r2 values of 0.80, 0.85, and 0.77 and Nash Sutcliffe values of 0.78, 0.70, and 0.77 for the cover, plant height, and biomass, respectively. The approach for estimating plant height and biomass did not work for sites where forbs comprised more than 30% of total vegetative cover. The ground reconnaissance protocol and image processing techniques together offer land managers accurate and timely methods for monitoring extensive grasslands. The time-consuming requirement to collect concurrent data in the field for each image implies a need to share the high fixed costs of processing an image across multiple users to reduce the costs for individual rangeland managers.

Rangeland Ecology and Management

Monitoring Ground-Water Quality in Coastal Ecosystems

INTRODUCTION The Cape Cod National Seashore (CACO) extends along more than 70 km of Atlantic Ocean open-beach coastline and includes three large saltwater bays - Wellfleet Harbor, Nauset Marsh, and Pleasant Bay (fig. 1). CACO encompasses about 18,000 ha of uplands, lakes, wetlands, and tidal lands (Godfrey and others, 1999) including most habitats typical of the sandy coast in National seashores and parks extending southward from Massachusetts to Florida. In 1995, CACO was selected by the National Park Service (NPS) as a prototype park typifying the Atlantic and Gulf Coast biogeographic region for long-term coastal ecosystem monitoring. The U.S. Geological Survey (USGS) is currently (2007) assisting the NPS in the development of protocols for a Long-Term Coastal Ecosystem Monitoring Program at the CACO in Massachusetts. The overall purpose of the monitoring program is to characterize both natural and human-induced change in the biological resources of the CACO, over a time scale of decades, in the context of a changing global ecosystem.

Open-File Report

The Wisconsin Frog and Toad Survey: Update and 1984-97 trends [abstract]

The Wisconsin Frog and Toad Survey (WFTS) is a volunteer-based, roadside auditory count that began in 1981. It's protocols were recently modified for continent-wide use by the North American Amphibian Monitoring Plan (NAAMP). In 1997 we initiated a study to compare data collected by the WFTS and NAAMP protocols, in order to guide WFTS transition from its current methodology to one more compatible with NAAMP, without losing the use of data collected since 1981. In this paper we present results from the first year of this study, along with results from analyses of WFTS data, including distributional maps, 1984-97 population trends, phenological information, and progress on a new web page.

Book chapter

Assessment of general health of fishes collected at selected sites in the Great Lakes Basin In 2012

During the past decade, there has been a substantive increase in the detection of “emerging contaminants”, defined as a new substance, chemical, or metabolite in the environment; or a legacy substance with a newly expanded distribution, altered release, or a newly recognized effect (such as endocrine disruption). Emerging contaminants include substances such as biogenic hormones (human and animal), brominated flame retardants, pharmaceuticals, personal care products, plasticizers, current use pesticides, detergents, and nanoparticles. These contaminants are frequently not regulated or inadequately regulated by state or Federal water quality programs. Information about the toxicity of these substances to fish and wildlife resources is generally limited, compared to more highly regulated contaminants, and some classes have been shown to cause affects (for example feminization of male fish, immunomodulation) that are not evaluated via traditional toxicity testing protocols. As a result, these compounds may pose a substantial, but currently poorly documented threat to aquatic ecosystems. Failure to identify and understand the impacts of these emerging contaminants on fish and wildlife resources may result in deleterious impacts to Great Lakes resources that can result in adverse ecological, economic and recreational consequences. The U. S. Fish and Wildlife Service received funding through the Great Lakes Restoration Initiative (GLRI) for an Early Warning Program to detect and identify emerging contaminants and to evaluate the effects of these contaminants on fish and wildlife. The U.S. Geological Survey (WV Cooperative Fish and Wildlife Research Unit and National Fish Health Research Laboratory, Leetown Science Center) developed and implemented a biological effects monitoring protocol to assist in this program. Fish collections and measurements of biomarkers of exposure in Fall 2010 and Spring 2011 occurred at individual sites within select Areas of Concern (AOCs). They provided an assessment of the utility of the suite of biomarkers and also identified sites for more in-depth analyses. Selected areas are characterized as areas with known emerging contaminants, sensitive or listed species, areas downstream from municipal wastewater discharges or receiving waters for industrial facilities, and/or areas susceptible to agricultural or urban contamination, or harbors or ports. The results of the 2010- 2011 studies were summarized in Blazer et al. 2014 a, b, c; Braham et al. in review and Blazer et al. in review.

Great Lakes

Use of set blanks in reporting pesticide results at the U.S. Geological Survey National Water Quality Laboratory, 2001-15

Executive Summary Background .—Pesticide results from the U.S. Geological Survey (USGS) National Water Quality Laboratory (NWQL) are used for water-quality assessments by many agencies and organizations. The USGS is committed to providing data of the highest possible quality to the consumers of its data. A cooperator’s inquiries about specific pesticide detections in water revealed potential laboratory contamination issues for some results. Consequently, the USGS conducted an extensive evaluation of potential low-level contamination related to processing or analysis of water-quality samples at NWQL for 21 pesticide compounds of interest to the cooperator. This is the most comprehensive study of NWQL pesticide quality-control (QC) results to date. Purpose and scope .—The purpose of this study was to document protocols used by the NWQL to censor pesticide results and to determine the effects of laboratory contamination—as determined from detections in laboratory set blanks—on pesticide detections in groundwater and surface-water samples. More than 30,000 pesticide results from 113 selected batches of samples (2 percent or less of total batches) analyzed by the NWQL during the 15 years from 2001 to 2015 were reviewed. All laboratory results from the selected batches, including results from environmental (surface water and groundwater) and QC (set-blank, blind-blank, and blind-spike) samples, were evaluated. The study includes results for 21 pesticide compounds analyzed in groundwater and surface-water samples collected across the United States. Eleven pesticide compounds were analyzed by a gas chromatography/mass spectrometry method and 10 compounds by a liquid chromatography/mass spectrometry method. Objectives and methods .—The objectives of this study were to (1) determine the characteristics of laboratory contamination over time, (2) compare distributions of pesticide results in set blanks with distributions in environmental samples, (3) evaluate the potential for false-positive and false-negative reporting of results, and (4) evaluate the effects of reevaluating historical pesticide results using 2017 compound identification protocols on detections of pesticides in groundwater and surface-water samples. The 113 instrument batches selected for this study contained detections of one or more of the 21 pesticide compounds in set blanks or were among those batches with the highest pesticide detection frequencies in set blanks. As a result, the dataset for this study was targeted toward pesticides and batches with laboratory contamination. The objectives were addressed by statistically comparing environmental and set-blank results; computing moving averages of set-blank detection frequencies to identify periods of episodic contamination; and using summary statistics, tabular summaries, and graphical approaches, such as time-series plots and cumulative distribution functions. Results .— Objective 1 : Laboratory contamination, as determined by pesticide detections in set blanks, was found in 13 percent of set-blank results from the 113 targeted batches included in this study (as compared to 6 percent of set-blank results from all 7,620 batches analyzed during the study period). It is estimated that 92 percent of the laboratory contamination during the study period was episodic, meaning that it occurred during discrete periods of time. All 21 of the targeted pesticide compounds had periods of episodic contamination, with most episodes ranging in duration from about 1 to 8 months. The remaining 8 percent of laboratory contamination was random or from a known source (deterministic). Objective 2 : For some compounds, graphs of cumulative distribution functions of the entire distributions of set-blank and environmental samples overlap, suggesting that there is no difference in the distributions of the two types of samples. However, time-series graphs show that detections in set blanks often occur at different times (sometimes separated by years) than detections in environmental samples, indicating clear differences in those distributions, and indicating the importance of evaluating the timing of detections in all sample types. For most compounds detected in set-blank and environmental samples, detection frequencies were significantly greater in set blanks than in groundwater or surface-water samples ( p <0.05). There are several explanations for this finding, including that the 113 batches of samples chosen for this study targeted batches with detections in set blanks or that detections in set-blank samples were historically determined with less stringent identification criteria than for environmental samples (groundwater and surface-water samples). Objective 3 : The false-positive and false-negative rates from blind samples submitted during the study period by the USGS Quality Systems Branch generally were less than 1 and 5 percent, respectively, for the 21 pesticides. The only compound with a false-positive rate greater than 1 percent was flumetsulam (2.6 percent), indicating that there is a higher likelihood of flumetsulam being reported as a detection when it is not present in an environmental sample compared with the reporting of other compounds. Objective 4 : Altogether, for data in targeted batches, NWQL would have reported 0.1 percent of results from groundwater samples and 1.4 percent of results from surface-water samples differently if 2017 identification protocols were applied to historical pesticide results. In most of these cases, detections observed in historical results would change to nondetections. The small percentages of changes that would occur if historical data were reevaluated indicate that historical protocols used by the NWQL to identify detections in environmental samples were robust and produced results that are predominantly consistent with current [2017] practices. Conclusions .—The NWQL produces high-quality pesticide results at environmentally relevant concentrations. NWQL identification protocols and censoring practices are largely effective at minimizing the reporting of false-positive and false-negative results. Laboratory contamination, when it occurred, tended to occur in episodes; thus, evaluating the timing and magnitude of detections in set blanks relative to detections in environmental samples was determined to be an important consideration for analysis of environmental results. Because NWQL censoring practices do not address all types and occurrences of laboratory contamination, options for additional censoring practices are provided for data users with more specific or stringent data-quality objectives. The methods used to analyze the 21 compounds for this report can similarly be applied to all 173 pesticide compounds that were analyzed by the NWQL during the same time period. This study also has helped to identify potential improvements in reporting USGS data, such as conducting more frequent review of set-blank datasets.

Scientific Investigations Report

St. Petersburg Coastal and Marine Science Center's Core Archive Portal

This Web site contains information on rock cores archived at the U.S. Geological Survey (USGS) St. Petersburg Coastal and Marine Science Center (SPCMSC). Archived cores consist of 3- to 4-inch-diameter coral cores, 1- to 2-inch-diameter rock cores, and a few unlabeled loose coral and rock samples. This document - and specifically the archive Web site portal - is intended to be a 'living' document that will be updated continually as additional cores are collected and archived. This document may also contain future references and links to a catalog of sediment cores. Sediment cores will include vibracores, pushcores, and other loose sediment samples collected for research purposes. This document will: (1) serve as a database for locating core material currently archived at the USGS SPCMSC facility; (2) provide a protocol for entry of new core material into the archive system; and, (3) set the procedures necessary for checking out core material for scientific purposes. Core material may be loaned to other governmental agencies, academia, or non-governmental organizations at the discretion of the USGS SPCMSC curator.

Data Series

Evolutionary dynamics of an expressed MHC class IIβ locus in the Ranidae (Anura) uncovered by genome walking and high-throughput amplicon sequencing

The Major Histocompatibility Complex (MHC) is a genomic region encoding immune loci that are important and frequently used markers in studies of adaptive genetic variation and disease resistance. Given the primary role of infectious diseases in contributing to global amphibian declines, we characterized the hypervariable exon 2 and flanking introns of the MHC Class IIβ chain for 17 species of frogs in the Ranidae, a speciose and cosmopolitan family facing widespread pathogen infections and declines. We find high levels of genetic variation concentrated in the Peptide Binding Region (PBR) of the exon. Ten codons are under positive selection, nine of which are located in the mammal-defined PBR. We hypothesize that the tenth codon (residue 21) is an amphibian-specific PBR site that may be important in disease resistance. Trans-species and trans-generic polymorphisms are evident from exon-based genealogies, and co-phylogenetic analyses between intron, exon and mitochondrial based reconstructions reveal incongruent topologies, likely due to different locus histories. We developed two sets of barcoded adapters that reliably amplify a single and likely functional locus in all screened species using both 454 and Illumina based sequencing methods. These primers provide a resource for multiplexing and directly sequencing hundreds of samples in a single sequencing run, avoiding the labour and chimeric sequences associated with cloning, and enabling MHC population genetic analyses. Although the primers are currently limited to the 17 species we tested, these sequences and protocols provide a useful genetic resource and can serve as a starting point for future disease, adaptation and conservation studies across a range of anuran taxa.

Developmental and Comparative Immunology

The U.S. Geological Survey Geologic Collections Management System (GCMS)—A master catalog and collections management plan for U.S. Geological Survey geologic samples and sample collections

**Updated guidance is available in USGS Instructional Memorandum CSS 2019-01 .** Abstract The U.S. Geological Survey (USGS) is widely recognized in the earth science community as possessing extensive collections of earth materials collected by research personnel over the course of its history. In 2006, a Geologic Collections Inventory was conducted within the USGS Geology Discipline to determine the extent and nature of its sample collections, and in 2008, a working group was convened by the USGS National Geologic and Geophysical Data Preservation Program to examine ways in which these collections could be coordinated, cataloged, and made available to researchers both inside and outside the USGS. The charge to this working group was to evaluate the proposition of creating a Geologic Collections Management System (GCMS), a centralized database that would (1) identify all existing USGS geologic collections, regardless of size, (2) create a virtual link among the collections, and (3) provide a way for scientists and other researchers to obtain access to the samples and data in which they are interested. Additionally, the group was instructed to develop criteria for evaluating current collections and to establish an operating plan and set of standard practices for handling, identifying, and managing future sample collections. Policies and procedures promoted by the GCMS would be based on extant best practices established by the National Science Foundation and the Smithsonian Institution. The resulting report—USGS Circular 1410, “The U.S. Geological Survey Geologic Collections Management System (GCMS): A Master Catalog and Collections Management Plan for U.S. Geological Survey Geologic Samples and Sample Collections”—has been developed for sample repositories to be a guide to establishing common practices in the collection, retention, and disposal of geologic research materials throughout the USGS. While constructing this report, the GCMS’s potential customers and their needs were considered. Two critical definitions have been clarified: a repository is a facility for the long-term management of geologic collections, and a collection is a set of specimens that have been brought together on the basis of some common characteristic. Required sample metadata for newly collected samples, as well as for older collections, were also stipulated and are listed and explained in this report. Several basic policies are also recommended by the GCMS in order to standardize operations among the physical repositories where collections are currently housed. The GCMS Collection Management Plan provides a set of protocols and templates for the management of scientific collections, including access, storage, transfer, and disposal of physical geologic samples and data. This plan is flexible to allow each repository to adapt the practices best suited to its collections. The general consideration for implementation of the GCMS is that all active USGS geologic sample repositories will form the core of GCMS and that participating science centers will develop procedures based on proposed GCMS methodologies. The GCMS is a collective resource for the entire USGS community and the users who discover the geologic materials kept in these repositories and seek to access them.

Circular

Review of Results and Recommendations from the GCMRC 2000-2003 Remote-Sensing Initiative for Monitoring Environmental Resources Within the Colorado River Ecosystem

In mid-2000, the Grand Canyon Monitoring and Research Center (GCMRC) began a remote-sensing initiative to evaluate all remote-sensing technologies and methods that had potential for providing improved data (capability) for its various programs that monitor the Colorado River ecosystem (CRE). The primary objective of the initiative was to determine the most cost-effective data collection protocols for GCMRC programs that (1) provide the accuracies required for currently measured parameters, (2) provide additional parameters for ecological monitoring, (3) reduce environmental impact by being less invasive than current methods, and (4) expand geographic extent of current ground approaches. The initial phase of the remote-sensing initiative determined the types of sampling parameters and their required accuracies for monitoring. This information was used to determine the most appropriate sensors for evaluation. The initiative evaluated 25 different data collections over a three-year period; many more remote-sensing instruments were considered, but were not evaluated because they could not meet the basic requirements on spatial resolution, wavelength, positional accuracy, or elevation accuracy. It was hoped that the evaluations would lead to a minimum set of technologies that would satisfy many program requirements. The results from all of our evaluations are reviewed in this report and are briefly summarized in this report.

Open-File Report

Winter bird population studies and project prairie birds for surveying grassland birds

We compared 2 survey methods for assessing winter bird communities in temperate grasslands: Winter Bird Population Study surveys are area-searches that have long been used in a variety of habitats whereas Project Prairie Bird surveys employ active-flushing techniques on strip-transects and are intended for use in grasslands. We used both methods to survey birds on 14 herbaceous reforested sites and 9 coastal pine savannas during winter and compared resultant estimates of species richness and relative abundance. These techniques did not yield similar estimates of avian populations. We found Winter Bird Population Studies consistently produced higher estimates of species richness, whereas Project Prairie Birds produced higher estimates of avian abundance for some species. When it is important to identify all species within the winter bird community, Winter Bird Population Studies should be the survey method of choice. If estimates of the abundance of relatively secretive grassland bird species are desired, the use of Project Prairie Birds protocols is warranted. However, we suggest that both survey techniques, as currently employed, are deficient and recommend distance- based survey methods that provide species-specific estimates of detection probabilities be incorporated into these survey methods.

Southeastern Naturalist

Reach-scale comparison of habitat and mollusk assemblages for select sites in the Clinch River with regional context

Several hypotheses, including habitat degradation and variation in fluvial geomorphology, have been posed to explain extreme spatial and temporal variation in Clinch River mollusk assemblages. We examined associations between mollusk assemblage metrics (richness, abundance, recruitment) and physical habitat (geomorphology, streambed composition, fish habitat, and riparian condition) at 10 sites selected to represent the range of current assemblage condition in the Clinch River. We compared similar geomorphological units among reaches, employing semi-quantitative and quantitative protocols to characterize mollusk assemblages and a mix of visual assessments and empirical measurements to characterize physical habitat. We found little to no evidence that current assemblage condition was associated with 54 analyzed habitat metrics. When compared to other sites in the Upper Tennessee River Basin (UTRB) that once supported or currently support mollusk assemblages, Clinch River sites were more similar to each other, representing a narrower range of conditions than observed across the larger geographic extent of the UTRB. A post-hoc analysis suggested stream size and average boundary shear stress at bankfull stage may have historically limited species richness in the UTRB (p < 0.001). Associations between mollusk assemblages and physical habitat in the UTRB and Clinch River currently appear obscured by other factors limiting richness, abundance, and recruitment.

Tennessee, Virginia

Conditions and processes affecting sand resources at archeological sites in the Colorado River corridor below Glen Canyon Dam, Arizona

This study examined links among fluvial, aeolian, and hillslope geomorphic processes that affect archeological sites and surrounding landscapes in the Colorado River corridor downstream from Glen Canyon Dam, Arizona. We assessed the potential for Colorado River sediment to enhance the preservation of river-corridor archeological resources through aeolian sand deposition or mitigation of gully erosion. By identifying locally prevailing wind directions, locations of modern sandbars, and likely aeolian-transport barriers, we determined that relatively few archeological sites are now ideally situated to receive aeolian sand supply from sandbars deposited by recent controlled floods. Whereas three-fourths of the 358 river-corridor archeological sites we examined include Colorado River sediment as an integral component of their geomorphic context, only 32 sites currently appear to have a high degree of connectivity (coupled interactions) between modern fluvial sandbars and sand-dominated landscapes downwind. This represents a substantial decrease from past decades, as determined by aerial-photograph analysis. Thus, we infer that recent controlled floods have had a limited, and declining, influence on archeological-site preservation. Within the study area, overland-flow (gully) erosion is less severe in sand landscapes with active aeolian sand than in landscapes that lack aeolian transport; gullies terminate more commonly in active sand (sand that is mobile by wind rather than stabilized by biologic soil crust). We infer that these characteristics largely result from aeolian sand transport being an effective gully-limiting and gully-annealing mechanism. Aeolian sand activity in the river corridor varies substantially as a function of reach morphology and dominant wind direction relative to the river-corridor orientation, factors that control accommodation space for river-derived sand and the modern sand supply to aeolian dunes. These attributes, together with an inverse correlation between aeolian sand activity and gully occurrence, define varying degrees of net long-term gully-erosion risk for sediment deposits and associated archeological sites in different regions of the river corridor. Over most of the river corridor, including some of the archeologically richest regions, sand is too inactive with respect to aeolian transport to anneal gullies effectively. At eight selected archeological sites that we studied with high-resolution terrestrial lidar scans for more than a year, sand loss by overland flow (gully erosion) and aeolian deflation generally exceeded deposition, such that erosion dominated over most monitoring intervals&mdash;even at four sites with strong connectivity to modern sand supply. The Glen Canyon reach of the river corridor appears especially vulnerable to gully erosion. Among the sites that we monitored in detail, erosion generally dominated over deposition to a greater degree at four Glen Canyon sites with no modern sand supply than at four Marble&ndash;Grand Canyon sites with aeolian sand supply from controlled-flood sandbars. Although gross annual-scale erosion rates were similar among the Glen Canyon sites and among the Marble&ndash;Grand Canyon sites, a relative lack of depositional processes led to greater net erosion at the Glen Canyon sites. Having found no differences in weather patterns to suggest greater erosive forcing in Glen Canyon, and no conclusively influential differences in the slope or watershed area contributing to gully formation, we attribute the greater erosion at the Glen Canyon sites to a combination of inherent geomorphic context (high terraces that do not receive modern sediment supply) and pronounced effects of postdam sediment-supply limitation. We conclude that most of the river-corridor archeological sites are at elevated risk of net erosion under present dam operations. In the present flow regime, controlled floods do not simulate the magnitude or frequency of natural floods, and are not large enough to deposit sand at elevations that were flooded at annual to decadal intervals in predam time. For archeological sites that depend upon river-derived sand, we infer elevated erosion risk owing to a combination of reduced sand supply (both fluvial and aeolian) through (1) the lower-than-natural flood magnitude, frequency, and sediment supply of the controlled-flooding protocol; (2) reduction of open, dry sand area available for wind redistribution under current normal (nonflood) dam operations, which do not include flows as low as natural seasonal low flows and do include substantial daily flow fluctuations; and (3) impeded aeolian sand entrainment and transport owing to increased riparian vegetation growth in the absence of larger, more-frequent floods. If dam operations were to increase the supply of sand available for windblown transport&mdash;for example, through larger floods, sediment augmentation, or increased fluvial sandbar exposure by low flows&mdash;and also decrease riparian vegetation, the prevalence of active aeolian sand could increase over time, and the propensity for unmitigated gully erosion could decrease. Although the evolution of river-corridor landscapes and archeological sites has been altered fundamentally by the lack of large, sediment-rich floods (flows on the order of 5,000 m 3 /s), some combination of sediment-rich flows above 1,270 m 3 /s, seasonal flows below 226 m 3 /s, and riparian-vegetation removal might increase the preservation potential for sand-dependent archeological resources in the Colorado River corridor.

Arizona

Evaluation of chemical data from selected sites in the Surface-Water Ambient Monitoring Program (SWAMP) in Florida

A cooperative study between the Florida Department of Environmental Protection (FDEP) and the U.S. Geological Survey was conducted to assess the integrity of selected water-quality data collected at 150 sites in the FDEP Surface-Water Ambient Monitoring Program (SWAMP) in Florida. The assessment included determining the consistency of the water-quality data collected statewide, including commonality of monitoring procedures and analytes, screening of the gross validity of a chemical analysis, and quality assurance and quality control (QA/QC) procedures. Four tests were used to screen data at selected SWAMP sites to estimate the gross validity of selected chemical data: (1) the ratio of dissolved solids (in milligrams per liter) to specific conductance (in microsiemens per centimeter); (2) the ratio of total cations (in milliequivalents per liter) multiplied by 100 to specific conductance (in microsiemens per centimeter); (3) the ratio of total anions (in milliequivalents per liter) multiplied by 100 to specific conductance (in microsiemens per centimeter); and (4) the ionic charge-balance error. Although the results of the four screening tests indicate that the chemical data generally are quite reliable, the extremely small number of samples (less than 5 percent of the total number of samples) with sufficient chemical information to run the tests may not provide a representative indication of the analytical accuracy of all laboratories in the program. In addition to the four screening tests, unusually low or high values were flagged for field and laboratory pH (less than 4.0 and greater than 9.0) and specific conductance (less than 10 and greater than 10,000 microsiemens per centimeter). The numbers of flagged data were less than 1 percent of the 19,937 water samples with pH values and less than 0.6 percent of the 16,553 water samples with specific conductance values. Thirty-four agencies responded to a detailed questionnaire that was sent to more than 60 agencies involved in the collection and analysis of surface-water-quality data for SWAMP. The purpose of the survey was to evaluate quality assurance methods and consistency of methods statewide. Information was compiled and summarized on monitoring network design, data review and upload procedures, laboratory and field sampling methods, and data practices. Currently, most agencies that responded to the survey follow FDEP-approved QA/QC protocol for sampling and have quality assurance practices for recording and reporting data. Also, most agencies responded that calibration procedures were followed in the laboratory for analysis of data, but no responses were given about the specific procedures. Approximately 50 percent of the respondents indicated that laboratory analysis methods have changed over time. With so many laboratories involved in analyzing samples for SWAMP, it is difficult to compare water quality from one site to another due to different reporting conventions for chemical constituents and different analytical methods over time. Most agencies responded that calibration methods are followed in the field, but no specific details were provided. Grab samples are the most common method of collection. Other data screening procedures are necessary to further evaluate the validity of chemical data collected at SWAMP sites. High variability in the concentration of targeted constituents may signal analytical problems, but more likely changes in concentration are related to hydrologic conditions. This underscores the need for accurate measurements of discharge, lake stage, tidal stage at the time of sampling so that changes in constituent concentrations can be properly evaluated and fluxes (loads) of nutrients or metals, for example, can be calculated and compared over time.

Florida

A strategy for defining the reference for land health and degradation assessments

Much of the confusion about the definition of reference conditions for land health and degradation assessments is due to differences in policy and management objectives. Selection of a historic reference where it is not necessary, such as in the definition of future land degradation neutrality, can add significant cost and uncertainty to land management projects that require some knowledge of the current status of the land relative to its potential. This paper (1) provides a review of conditions under which historic reference information is and is not required to meet management and policy objectives, (2) summarizes current approaches to defining the reference for land health and degradation assessments, and (3) presents a protocol, “Describing Indicators of Rangeland Health” (DIRH) for collecting and organizing data that can be used to define a historic reference. This protocol builds on the framework and indicators presented in the “Interpreting Indicators of Rangeland Health” (IIRH). IIRH uses a combination of scientific and local knowledge to generate soil- and climate-specific assessments of three attributes of land health. It is used in a number of countries. In the United States, data are aggregated over 30,000 locations to provide national assessments.

Ecological Indicators

Global application of an unoccupied aerial vehicle photogrammetry protocol for predicting aboveground biomass in non-forest ecosystems

Non-forest ecosystems, dominated by shrubs, grasses and herbaceous plants, provide ecosystem services including carbon sequestration and forage for grazing, and are highly sensitive to climatic changes. Yet these ecosystems are poorly represented in remotely sensed biomass products and are undersampled by in situ monitoring. Current global change threats emphasize the need for new tools to capture biomass change in non-forest ecosystems at appropriate scales. Here we developed and deployed a new protocol for photogrammetric height using unoccupied aerial vehicle (UAV) images to test its capability for delivering standardized measurements of biomass across a globally distributed field experiment. We assessed whether canopy height inferred from UAV photogrammetry allows the prediction of aboveground biomass (AGB) across low-stature plant species by conducting 38 photogrammetric surveys over 741 harvested plots to sample 50 species. We found mean canopy height was strongly predictive of AGB across species, with a median adjusted R 2 of 0.87 (ranging from 0.46 to 0.99) and median prediction error from leave-one-out cross-validation of 3.9%. Biomass per-unit-of-height was similar within but different among, plant functional types. We found that photogrammetric reconstructions of canopy height were sensitive to wind speed but not sun elevation during surveys. We demonstrated that our photogrammetric approach produced generalizable measurements across growth forms and environmental settings and yielded accuracies as good as those obtained from in situ approaches. We demonstrate that using a standardized approach for UAV photogrammetry can deliver accurate AGB estimates across a wide range of dynamic and heterogeneous ecosystems. Many academic and land management institutions have the technical capacity to deploy these approaches over extents of 1–10 ha −1 . Photogrammetric approaches could provide much-needed information required to calibrate and validate the vegetation models and satellite-derived biomass products that are essential to understand vulnerable and understudied non-forested ecosystems around the globe.

Remote Sensing in Ecology and Conservation

Water quality monitoring protocol for wadeable streams and rivers in the Northern Great Plains Network

Preserving the national parks unimpaired for the enjoyment of future generations is a fundamental purpose of the National Park Service (NPS). To address growing concerns regarding the overall physical, chemical, and biological elements and processes of park ecosystems, the NPS implemented science-based management through “Vital Signs” monitoring in 270 national parks (NPS 2007). The Northern Great Plains Network (NGPN) is among the 32 National Park Service Networks participating in this monitoring effort. The NGPN will develop protocols over the next several years to determine the overall health or condition of resources within 13 parks located in Nebraska, North Dakota, South Dakota, and Wyoming. The NGPN identified water resources as a Vital Sign to monitor because water quality and quantity are important aspects of ecological processes that operate across multiple temporal and spatial scales. In the semi-arid region of the Northern Great Plains, surface-water resources within the NGPN are ecologically important. The 13 parks within the NGPN are diverse and vary greatly in size, visitation, and water resources. For example, the measured surface area of the Badlands National Park is about 243,000 acres, which represents nearly one-half of the combined acreage of all 13 NGPN park units; however, water resources within the park are scarce and the majority of streams are intermittent. The Badlands National Park annually hosts nearly 860,000 visitors. Mount Rushmore National Memorial also has limited water resources but hosts nearly 3 million visitors per year within its 1,278 acres. The Missouri National Recreational River contains the greatest portion of waterbodies within the NGPN, consisting of 139 rivers and streams within an areal extent of about 69,000 acres. Although water resources and acreage of the NGPN parks are varied, unifying factors among the parks include the relatively low population density within the Great Plains area and the strong emphasis on agrarian land use throughout the region. To address the diverse water quality concerns, NGPN received input from park staff and conducted pilot studies in 2009 and 2010. These factors, in combination with the NGPN budget allocations, resulted in development of the NGPN’s water quality monitoring protocol. This protocol will provide a context to aid park resource managers in their day-to-day decisions and allow the assessment of the status (current conditions) and trends (directional changes across time) of streams/rivers within selected NGPN parks. Data collected from integrating water resource monitoring, in combination with the inventory of additional Vital Signs, can be used to assess resources and to aid in sound managerial decisions by the NGPN parks. As recommended by Oakley et al. (2003), this protocol provides a narrative and the rationale for selection of streams and rivers within the NGPN that will be measured for water quality, including dissolved oxygen, pH, specific conductivity, and temperature. Standard operating procedures (SOPs) that detail the steps to collect, manage, and disseminate the NGPN water quality data are in an accompanying document. The sampling design documented in this protocol may be updated as monitoring information is collected and interpreted, and as refinement of methodologies develop through time. In addition, evaluation of data and refinement of the program may necessitate potential changes of program objectives. Changes to the NGPN water quality protocols and SOPs will be carefully documented in a revision history log.

Colorado, Montana, Nebraska, North Dakota, South D