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Biogeography of freshwater mussels (Bivalvia: Unionida) in Texas and implications on conservation biology

Aim Biogeography seeks to identify and explain the spatial distributions of species and has become an important tool used by conservationists to protect and manage aquatic organisms. Texas, located in the southwestern United States, is home to 52 species of freshwater mussels, 9 of which are endemic to Texas and 7 that are endemic to Texas and neighboring states or countries. There have been two major attempts to classify this fauna into biogeographical provinces; however, both efforts relied on limited distribution information and outdated taxonomy. To address both issues, we set out to delineate biogeographic provinces for freshwater mussels in Texas by using a comprehensive distributional dataset of >28,000 records and molecular information. Location Southwestern United States. Methods We compiled community and molecular data for 48 of the 52 freshwater mussel species that occur in Texas. We performed algorithmic hierarchal cluster analysis (HCA) and nonmetric multidimensional scaling (NMDS) based on Euclidean distance to identify biogeographic groupings. We conducted a similar analysis using molecular sequence data for our target species. Results Based on the results from community and molecular data, we identified seven biogeographic provinces for freshwater mussels in Texas: Great Plains, Mississippi Embayment, Sabine-Neches, Trinity-San Jacinto, Central Texas, Rio Grande and Coastal. However, the Coastal and Great Plains provinces were not included in our analysis and were recognized based on previous work. Main conclusions Our approach integrating community and molecular datasets provides a comprehensive assessment of the biogeography of freshwater mussels in Texas, which serves as a model for future biogeographic studies. Our findings also shed light on the ecological, evolutionary and geologic processes shaping freshwater mussel communities in Texas, which is important for the conservation of remaining biodiversity in the state.

Texas

Modeling the supporting ecosystem services of depressional wetlands

We explored how a geographic information system modeling approach could be used to quantify supporting ecosystem services related to the type, abundance, and distribution of landscape components. Specifically, we use the Integrated Valuation of Ecosystem Services and Tradeoffs model to quantify habitats that support amphibians and birds, floral resources that support pollinators, native-plant communities that support regional biodiversity, and above- and below-ground carbon stores in the Des Moines Lobe ecoregion of the U.S. We quantified services under two scenarios, one that represented the 2012 Des Moines Lobe landscape, and one that simulated the conversion to crop production of wetlands and surrounding uplands conserved under the USDA Agricultural Conservation Easement Program (ACEP). While ACEP easements only covered 0.35% of the ecoregion, preserved wetlands and grasslands provided for 19,020 ha of amphibian habitat, 21,462 ha of grassland-bird habitat, 18,798 ha of high-quality native wetland plants, and 27,882 ha of floral resources for pollinators. Additionally, ACEP protected lands stored 257,722 tonnes of carbon that, if released, would result in costs in excess of 45-million USD. An integrated approach using results from a GIS-based model in combination with process-based model quantifications will facilitate more informed decisions related to ecosystem service tradeoffs.

Iowa, Minnesota

Fish assemblage responses to water withdrawals and water supply reservoirs in Piedmont streams

Understanding effects of flow alteration on stream biota is essential to developing ecologically sustainable water supply strategies. We evaluated effects of altering flows via surface water withdrawals and instream reservoirs on stream fish assemblages, and compared effects with other hypothesized drivers of species richness and assemblage composition. We sampled fishes during three years in 28 streams used for municipal water supply in the Piedmont region of Georgia, U.S.A. Study sites had permitted average withdrawal rates that ranged from < 0.05 to > 13 times the stream’s seven-day, ten-year recurrence low flow (7Q10), and were located directly downstream either from a water supply reservoir or from a withdrawal taken from an unimpounded stream. Ordination analysis of catch data showed a shift in assemblage composition at reservoir sites corresponding to dominance by habitat generalist species. Richness of fluvial specialists averaged about 3 fewer species downstream from reservoirs, and also declined as permitted withdrawal rate increased above about 0.5 to one 7Q10-equivalent of water. Reservoir presence and withdrawal rate, along with drainage area, accounted for 70% of the among-site variance in fluvial specialist richness and were better predictor variables than percent of the catchment in urban land use or average streambed sediment size. Increasing withdrawal rate also increased the odds that a site’s Index of Biotic Integrity score fell below a regulatory threshold indicating biological impairment. Estimates of reservoir and withdrawal effects on stream biota could be used in predictive landscape models to support adaptive water supply planning intended to meet societal needs while conserving biological resources.

Georgiq

Evaluation of passive samplers for cyanotoxin detection by immunoassay and chromatographic-mass spectrometry

Harmful algal blooms, particularly cyanobacterial harmful algal blooms, threaten aquatic ecosystems, drinking water supplies, and recreational resources. In 2019, the U.S. Geological Survey, in collaboration with the New York State Department of Environmental Conservation, deployed solid phase adsorption toxin tracking (SPATT) samplers in Seneca Lake, Owasco Lake, and Skaneateles Lake to monitor the cyanotoxins microcystins, cylindrospermopsins, anatoxins, and saxitoxins. SPATT samplers can passively adsorb dissolved cyanotoxins over time, providing time-integrated data capable of detecting low concentrations of cyanotoxins that traditional discrete sampling may miss. SPATT samples were analyzed using enzyme-linked immunosorbent assay (ELISA), liquid chromatography with mass spectrometry (LC–MS), and with tandem mass spectrometry (LC–MS/MS). The effects of ELISA-required preservative on measurements by mass spectrometry methods were also evaluated. SPATT samplers consistently detected microcystins and anatoxins more frequently than concurrent discrete sampling. ELISA results often showed higher cyanotoxin concentrations than LC–MS/MS, likely due to interference from dissolved organic matter and the ability of ELISA to detect a broader range of congeners. The addition of preservative influenced results for some analytes, particularly microcystins, which showed higher concentrations in preserved samples. Limitations in ELISA methods for cylindrospermopsins and saxitoxins were identified, potentially related to cross-reactivity, low sensitivity, or other matrix interferences. This study demonstrates the utility of SPATT samplers in capturing cyanotoxin variability, especially in environments with low cyanotoxin levels or ephemeral blooms. Further research could help improve the reliability of ELISA and other analytical methods in freshwater ecosystems.

New York

Circumpolar Biodiversity Monitoring Programme coastal biodiversity monitoring background paper

In 2014, the United States (U.S.) and Canada agreed to act as co-lead countries for the initial development of the Coastal Expert Monitoring Group (CEMG) as part of the Circumpolar Biodiversity Monitoring Program (CBMP, www. cbmp.is) under the Arctic Council’s Conservation of Arctic Flora and Fauna (CAFF, www.caff.is) working group. The CAFF Management Board approved Terms of Reference for the CEMG in the spring of 2014. The primary goal of the CEMG is to develop a long term, integrated, multi-disciplinary, circumpolar Arctic Coastal Biodiversity Monitoring Plan (the Coastal Plan) that relies on science and Traditional Knowledge, and has direct and relevant application for communities, industry, government decision makers, and other users. In addition to the monitoring plan, the CAFF working group has asked the CBMP, and thus the CEMG, to develop an implementation plan that identifies timeline, costs, organizational structure and partners. This background paper provides a platform for the guidance for the development of the Coastal Plan and is produced by the CEMG with assistance from a number of experts in multiple countries.

Report

Genetic conservation and paddlefish propagation

The conservation of genetic diversity of our natural resources is overwhelmingly one of the central foci of 21st century management practices. Three recommendations related to the conservation of paddlefish Polyodon spathula genetic diversity are to (1) identify genetic diversity at both nuclear and mitochondrial DNA loci using a suggested list of 20 sampling locations, (2) use genetic diversity estimates to develop genetic management units, and (3) identify broodstock sources to minimize effects of supplemental stocking on the genetic integrity of native paddlefish populations. We review previous genetic work on paddlefish and described key principles and concepts associated with maintaining genetic diversity within and among paddlefish populations and also present a genetic case study of current paddlefish propagation at the U.S. Fish and Wildlife Service Gavins Point National Fish Hatchery. This study confirmed that three potential sources of broodfish were genetically indistinguishable at the loci examined, allowing the management agencies cooperating on this program flexibility in sampling gametes. This study also showed significant bias in the hatchery occurred in terms of male reproductive contribution, which resulted in a shift in the genetic diversity of progeny compared to the broodfish. This shift was shown to result from differential male contributions, partially attributed to the mode of egg fertilization. Genetic insights enable implementation of a paddlefish propagation program within an adaptive management strategy that conserves inherent genetic diversity while achieving demographic goals.

American Fisheries Society Symposium

Spatial demographic models to inform conservation planning of golden eagles in renewable energy landscapes

Spatial demographic models can help guide monitoring and management activities targeting at-risk species, even in cases where baseline data are lacking. Here, we provide an example of how site-specific changes in land use and anthropogenic stressors can be incorporated into a spatial demographic model to investigate effects on population dynamics of Golden Eagles ( Aquila chrysaetos ). Our study focused on a population of Golden Eagles exposed to risks associated with rapid increases in renewable energy development in southern California, U.S.A. We developed a spatially explicit, individual-based simulation model that integrated empirical data on demography of Golden Eagles with spatial data on the arrangement of nesting habitats, prey resources, and planned renewable energy development sites. Our model permitted simulated eagles of different stage-classes to disperse, establish home ranges, acquire prey resources, prospect for breeding sites, and reproduce. The distribution of nesting habitats, prey resources, and threats within each individual's home range influenced movement, reproduction, and survival. We used our model to explore potential effects of alternative disturbance scenarios, and proposed conservation strategies, on the future distribution and abundance of Golden Eagles in the study region. Results from our simulations suggest that probable increases in mortality associated with renewable energy infrastructure (e.g., collisions with wind turbines and vehicles, electrocution on power poles) could have negative consequences for population trajectories, but that site-specific conservation actions could reduce the magnitude of negative effects. Our study demonstrates the use of a flexible and expandable modeling framework to incorporate spatially dependent processes when determining relative effects of proposed management options to Golden Eagles and their habitats.

California

Managing the livestock– Wildlife interface on rangelands

On rangelands the livestock–wildlife interface is mostly characterized by management actions aimed at controlling problems associated with competition, disease, and depredation. Wildlife communities (especially the large vertebrate species) are typically incompatible with agricultural development because the opportunity costs of wildlife conservation are unaffordable except in arid and semi-arid regions. Ecological factors including the provision of supplementary food and water for livestock, together with the persecution of large predators, result in livestock replacing wildlife at biomass densities far exceeding those of indigenous ungulates. Diseases are difficult to eradicate from free-ranging wildlife populations and so veterinary controls usually focus on separating commercial livestock herds from wildlife. Persecution of large carnivores due to their depredation of livestock has caused the virtual eradication of apex predators from most rangelands. However, recent research points to a broad range of solutions to reduce conflict at the livestock–wildlife interface. Conserving wildlife bolsters the adaptive capacity of a rangeland by providing stakeholders with options for dealing with environmental change. This is contingent upon local communities being empowered to benefit directly from their wildlife resources within a management framework that integrates land-use sectors at the landscape scale. As rangelands undergo irreversible changes caused by species invasions and climate forcings, the future perspective favors a proactive shift in attitude towards the livestock–wildlife interface, from problem control to asset management.

Book chapter

Joint-species analysis reveals potential displacement of native fishes by non-native fishes within the Santa Ana River, California

Accurate estimates of abundance are a cornerstone for resource managers to make effective decisions for fish conservation. However, multiple sampling methods often are required to sample fish communities, and ignoring the detection process can create substantial bias in latent state parameter estimation (e.g., abundance, survival). We developed a joint-species N -mixture model that integrated snorkel, seining, and electrofishing surveys to estimate factors affecting native and non-native fish distributions in the Santa Ana River, California. We found through data integration that native Santa Ana sucker ( Catostomus santaanae ) and arroyo chub ( Gila orcuttii ) were most abundant in wide stream channels, and the abundance of both native fishes were negatively correlated with non-native largemouth bass ( Micropterus salmoides ). Our results highlight the power of integrating multiple data sets into a single analysis and incorporating among-species correlation into abundance modeling. Our results also highlight a pattern of native fish declines that coincides with an expanding largemouth bass population, a concern for the management of native aquatic communities within the Santa Ana River.

California

AIMS for wildlife: Developing an automated interactive monitoring system to integrate real-time movement and environmental data for true adaptive management

To effectively manage species and habitats at multiple scales, population and land managers require rapid information on wildlife use of managed areas and responses to landscape conditions and management actions. GPS tracking studies of wildlife are particularly informative to species ecology, habitat use, and conservation. Combining GPS data with administrative data and a diverse suite of remotely sensed, geo-referenced environmental (e.g., climatic) data, would more comprehensively inform how animals interact with and utilize habitats and ecosystems and our goal was to create a conceptual model for a system that would accomplish this – the ‘Automated Interactive Monitoring System (AIMS) for Wildlife’. Our objective for this study was to develop a Customized Wildlife Report (CWR) - the first AIMS for Wildlife deliverable product. CWRs collate and summarize our 8-year GPS tracking dataset of ∼11 million locations from 1338 individual (16 species) avifauna and make actionable, real-time data on animal movements and trends in a specific area of interest available to managers and stakeholders for rapid application in day-to-day management. The CWR exemplar presented in this paper was developed to address needs identified by habitat managers of Sacramento National Wildlife Refuge and illustrates the highly specific, information offered and how it contributes to assessing the efficacy of conservation actions while allowing for near real-time adaptive management. The report can be easily customized for any of the thousands of wildlife refuges or regional areas of interest in the United States, emphasizing the broad application of an animal movement data stream. Utilizing diverse, extensive telemetry data streams through scientific collaboration can aid managers and conservation stakeholders with short and long-term research and conservation planning and help address a cadre of issues from local-scale habitat management to improving the understanding of landscape level impacts like drought, wildfire, and climate change on wildlife populations.

Journal of Environmental Management

Integrating Sr isotopes, microchemistry, and genetics to reconstruct Salmonidae species and life history

Recent approaches to fisheries research emphasize the importance of the coproduction of knowledge in building resilient and culturally mindful fisheries management frameworks. Despite widespread recognition of the need for Indigenous knowledge and historical reference points as baseline data, archaeological data are rarely included in conservation biology research designs. Here we propose a novel multiproxy method to learn from former fisheries stewards by generating archaeological data on past salmonid population parameters. We used a newly developed, high throughput qPCR (HT-qPCR) chip, originally designed for environmental DNA (eDNA), for species identification of archaeological salmonid vertebrae. We combine this with the laser ablation split-stream (LASS) approach to identify ocean-migration versus freshwater residency. We test this multidisciplinary approach using both contemporary and archaeological salmonid samples and new radiocarbon dates from the Tronsdal Site on the Skagit River, Washington State, USA. This is a useful approach for extracting information about Salmonidae species and life history diversity from archaeological remains to reconstruct historic baselines for several population parameters in anadromous species with long periods of freshwater residency. The approach outlined in this paper may be particularly useful for research investigating past fisheries dynamics, offering hundreds to thousands of years of temporal depth for modern fisheries management, harvest policies, restoration ecology, and conservation biology.

Idaho, Oregon, Washington

Gulf Islands National Seashore regional sediment budget research and data needs—Workshop series summary

Executive Summary The National Park Service (NPS), in collaboration with the U.S. Geological Survey (USGS), recognizes the need to quantify the sediment budget of the barrier islands within the Gulf Islands National Seashore (GINS) to understand the coastal processes affecting island resiliency. To achieve this goal, identifying and quantifying the physical parameters that drive long-term change is necessary to model the processes that are both generative and terminal in island evolution and capture island response to long-term human alteration and climatic patterns. For example, measuring change across periods of storminess is more effective at assessing island resiliency than measuring change resulting from a single storm impact. Understanding changes to the physical environment over time is key to successfully predicting island responses to future storm impacts, human alteration, and sea-level rise and is necessary for effective decision making and management response. Yet, the diversity of factors affecting natural and cultural resources necessitates a strategic approach to data collection priorities that can inform sediment budget quantification and integrated resource management. This study sought to advance sediment budget modeling efforts by conducting a “Needs Assessment Workshop” at the GINS. The purpose of the workshop was to identify and prioritize the specific research and data needs regarding the sediment budget at the GINS that can enhance the NPS efforts to conserve the islands’ natural resources, cultural resources, and the facilities and infrastructure that support both conservation and visitor use of those resources. This effort explored two research questions: (1) “what research and data needs exist for the sediment budget at Gulf Islands National Seashore” (research question 1) and (2) “how can research to address these needs capitalize on regional partnerships to advance natural and cultural resource conservation at Gulf Islands National Seashore” (research question 2)? The workshop was conducted virtually in a two-part, two-day series. The workshop series was organized by researchers from North Carolina State University in collaboration with NPS and USGS staff and was facilitated by National Oceanographic and Atmospheric Administration staff. The workshop series (two paired, sequential, partial-day workshops) addressed two target audiences: (1) NPS and USGS staff (April workshop) and (2) regional Federal, State, county, and nongovernmental organization staff, including NPS and USGS staff (May workshop). A total of four workshop sessions were held, comprising two sessions with each target audience. The workshop series intended to identify sediment management research and data needs that could enhance natural and cultural resource stewardship at the GINS. One objective was to share information about regional sediment transport and management, available sediment management plans, and predictive modeling capabilities, including geomorphologic and hydrodynamic predictive models. This information was shared through a series of presentations by park managers and NPS and USGS researchers that identified park issues and available capabilities and data. The second objective was to elicit research and data needs, with a primary goal of assessing the importance and urgency of the identified needs. This assessment was partly determined by requesting that the workshop participants identify and prioritize research themes through polls, comments, and discussion. The polls explicitly asked participants to qualitatively evaluate the importance (not at all, slightly, somewhat, very, or extremely) and urgency (not at all, slightly, somewhat, very, or extremely) of the thematically grouped research and data needs. These evaluations were plotted and shared during the workshop to visualize how the relative importance ( x -axis) and relative urgency ( y -axis) of each “need,” relative to other needs, to identify the most necessary (importance) and time-sensitive (urgent) items, thereby allowing an enhanced, holistic understanding of the sediment budget at GINS. Results of the poll are published as a USGS data release. The assessment results revealed that the most important and urgent research and data needs included mapping (for example, elevation, habitat, and cultural resources), a regional sediment budget and management plan, and the dynamic modeling of sediment processes. During the workshop, these issues were visualized using scatter plots to demonstrate the relative importance and urgency of each theme, provide descriptive statistics, and elicit discussion. This format of iterative presentation, discussion, and prioritization allowed the project team to effectively accomplish their objective of identifying important and urgent research needs for natural and cultural resource stewardship at the GINS. Through the workshop, it was determined that expanded communication with the broader research community was needed to coordinate research activities and streamline potential funding opportunities and that research and policy should be integrated through a structured decision-making process. At the conclusion of the workshop, an administered poll showed that the presentations effectively identified data and research needs and that the goals of the workshop were achieved. The results suggest that this type of needs-assessment workshop can effectively identify existing research capabilities and data, determine and prioritize research and data needs, and address how these efforts can use regional partnerships to aid natural and cultural resource conservation and management at National Parks.

Florida, Mississippi

LANDFIRE: Collaboration for National Fire Fuel Assessment

The implementation of national fire management policies, such as the National Fire Plan and the Healthy Forest Restoration Act, requires geospatial data of vegetation types and structure, wildland fuels, fire risks, and ecosystem fire regime conditions. Presently, no such data sets are available that can meet these requirements. As a result, the U.S. Department of Agriculture (USDA) Forest Service and the Department of the Interior's land management bureaus (Bureau of Indian Affairs (BIA), Bureau of Land Management (BLM), Fish and Wildlife Service (FWS), and National Park Service (NPS)) have jointly sponsored LANDFIRE, a new research and development project. The primary objective of the project is to develop an integrated and repeatable methodology and produce vegetation, fire, and ecosystem information and predictive models for cost-effective national land management applications. The project is conducted collaboratively by the U.S. Geological Survey (USGS), the USDA Forest Service, and The Nature Conservancy.

Fact Sheet

Northern Prairie Wildlife Research Center

The Northern Prairie Wildlife Research Center (NPWRC) conducts integrated research to fulfill the Department of the Interior's responsibilities to the Nation's natural resources. Located on 600 acres along the James River Valley near Jamestown, North Dakota, the NPWRC develops and disseminates scientific information needed to understand, conserve, and wisely manage the Nation's biological resources. Research emphasis is primarily on midcontinental plant and animal species and ecosystems of the United States. During the center's 40-year history, its scientists have earned an international reputation for leadership and expertise on the biology of waterfowl and grassland birds, wetland ecology and classification, mammalian behavior and ecology, grassland ecosystems, and application of statistics and geographic information systems. To address current science challenges, NPWRC scientists collaborate with researchers from other U.S. Geological Survey centers and disciplines (Biology, Geography, Geology, and Water) and with biologists and managers in the Department of the Interior (DOI), other Federal agencies, State agencies, universities, and nongovernmental organizations. Expanding upon its scientific expertise and leadership, the NPWRC is moving in new directions, including invasive plant species, restoration of native habitats, carbon sequestration and marketing, and ungulate management on DOI lands.

Fact Sheet

Multi-resource analysis: A proof of concept study of natural resource tradeoffs in the Piceance Basin, Colorado, using the net resources assessment (NetRA) decision support tool

Executive Summary The U.S. Geological Survey (USGS) is developing a multi-resource analysis (MRA) line of products to inform land-use decision makers. Specifically, MRA products will integrate scientific information, include considerations for natural resource interrelations, and quantify the effects of resource management decisions in biophysical, economic, and societal terms. As part of the establishment of the MRA, the USGS, in collaboration with the University of New Mexico, has developed the Net Resources Assessment (NetRA) decision support tool. As a proof of concept analysis, the NetRA was applied to the Piceance basin in Colorado in a hypothetical example to illustrate how resource managers could use the NetRA to consider tradeoffs of natural resources among alternative development plans and land cover patterns within a geographic region. The NetRA is a policy-relevant approach to assess the availability of multiple natural resources. It is an analytical toolset that may be used to examine the spatiotemporal relations between development of energy and mineral resources and delivery of biological natural resources. The NetRA operates at multiple map scales and contains a set of integrated, compatible submodels with specific data requirements for natural resource stocks, engineering economics, biophysical, and ecological data for ecosystem services stocks, market prices, regulations, and nonmarket values. The NetRA includes an explicit process to consider the interdependence between development and conservation, which is a crucial consideration in land-management and land-use decisions. The NetRA is used to estimate an expected net resource value (NRV). The NRV is the expected, present value, economic benefit from the extraction of a resource (for example, natural gas) minus the total cost of production, which is the aggregation of the development, production, and social costs. Social costs include private costs plus any external costs. There can be external social benefits associated with natural gas production, such as increased demand for locally produced goods and increased employment in the local area through backward and forward linkages of natural gas production. The NRV is used to compare development outcomes (scenarios) from a range of exploration and development plans for cumulative energy production. The Piceance basin application of the NetRA uses the NRV to assess the tradeoff between continuous natural gas extraction and the effects to the local populations of Odocoileus hemionus (mule deer) and aquatic species and to consumptive water uses for an area the size and resolution of a USGS energy resource assessment unit. In the proof of concept simulation, the 2.9-square-mile-area of USGS oil and gas assessment unit 50200263 (Piceance basin continuous gas unit of the Mesaverde Total Petroleum System) was gridded into 588 cells. From this area, seven clusters with potential for development and three that cannot be developed were identified; the three clusters that cannot be developed were identified as wilderness study areas, areas of critical environmental concern, and national forests. On the basis of these criteria, there are 118 cells unsuitable for development in the oil and gas assessment unit: 84 are in national forests, 23 are areas of critical environmental concern, and 11 are wilderness study areas. The remaining cells in the oil and gas assessment unit can be developed on both private and public lands. Two scenarios were considered that are distinguished as plan 1 and plan 2. Plan 1 keeps the amount of land disturbance unchanged and limits the number of development locations to 140 grid cells for the production period, which constrains the amount of the energy resources available for development; the plan requires the usage of the Bureau of Land Management (BLM) unsuitability criteria. Plan 2 also limits the number of development locations to 140 grid cells for the production period but provides a constant volume of energy production by increasing the density of well pads within the cells. The effects of plan 2 to the NRV when there are five wells per pad and five pads per square mile happen mostly in the first 5 years of development, even though the effects on the population of mule deer continue in later years. This outcome is the result of the upfront development and investment costs and the initial effect to the ecosystem services.

Colorado

An assessment of the representation of ecosystems in global protected areas using new maps of World Climate Regions and World Ecosystems

Representation of ecosystems in protected area networks and conservation strategies is a core principle of global conservation priority setting approaches and a commitment in Aichi Target 11 of the Convention on Biological Diversity. The 2030 Sustainable Development Goals (SDGs) explicitly call for the conservation of terrestrial, freshwater, and marine ecosystems. Accurate ecosystem distribution maps are required to assess representation of ecosystems in protected areas, but standardized, high spatial resolution, and globally comprehensive ecosystem maps have heretofore been lacking. While macroscale global ecoregions maps have been used in global conservation priority setting exercises, they do not identify distinct localized ecosystems at the occurrence (patch) level, and instead describe large ecologically meaningful areas within which additional conservation planning and management are necessary. We describe a new set of maps of globally consistent climate regions and ecosystems at a much finer spatial resolution (250 m) than existing ecological regionalizations. We then describe a global gap analysis of the representation of these ecosystems in protected areas. The new map of terrestrial World Ecosystems was derived from the objective development and integration of 1) global temperature domains, 2) global moisture domains, 3) global landforms, and 4) 2015 global vegetation and land use. These new terrestrial World Ecosystems do not include either freshwater or marine ecosystems, but analog products for the freshwater and marine domains are in development. A total of 431 World Ecosystems were identified, and of these a total of 278 units were natural or semi-natural vegetation/environment combinations, including different kinds of forestlands, shrublands, grasslands, bare areas, and ice/snow regions. The remaining classes were different kinds of croplands and settlements. Of the 278 natural and semi-natural classes, 9 were not represented in global protected areas with a strict biodiversity conservation management objective (IUCN management categories I-IV), and an additional 206 were less than 8.5% protected (half way to the 17% Aichi Target 11 goal). Forty four classes were between 8.5% and 17% protected (more than half way towards the Aichi 17% target), and only 19 classes exceeded the 17% Aichi target. However, when all protected areas (IUCN management categories I-VI plus protected areas with no IUCN designation) were included in a separate global gap analysis, representation of ecosystems increases substantially, with a third of the ecosystems exceeding the 17% Aichi target, and another third between 8.5% and 17%. The overall protection (representation) of global ecosystems in protected areas is considerably less when assessed using only strictly conserved protected areas, and more if all protected areas are included in the analysis. Protected area effectiveness should be included in further evaluations of global ecosystem protection. The ecosystems with the highest representation in protected areas were often bare or sparsely vegetated and found in inhospitable environments (e.g. cold mountains, deserts), and the eight most protected ecosystems were all snow and ice ecosystems. In addition to the global gap analysis of World Ecosystems in protected areas, we report on the representation results for the ecosystems in each biogeographic realm (Neotropical, Nearctic, Afrotropical, Palearctic, Indomalayan, Australasian, and Oceania).

Global Ecology and Conservation

Desert ecosystems shape diversification in glossy snakes (genus Arizona) requiring a re-alignment of evolutionary and conservation units

Subspecies are often targets for conservation, yet many lack the genetic data necessary to validate their status as distinctive evolutionary lineages. In 2016, conservationists faced this issue when designating the California glossy snake, Arizona elegans occidentalis , as a Species of Special Concern in California, a decision prompted by population declines and habitat loss but absent of genetic information about its evolutionary integrity. To address this knowledge gap, we collected genomic and mitochondrial data from a rangewide sample of the Arizona elegans complex ( n = 257) and characterized genetic structure at varying spatial scales. We confirmed an east–west phyletic division within the A. elegans complex that correlates with an ecotone between the Sonoran and Chihuahuan Deserts and pinpoint the separation to a ∼20 km area in southeastern Arizona, USA. Individuals recognized as A. e. occidentalis do not form a genetically cohesive unit within a more inclusive western clade that is sister to the endemic Arizona pacata in Baja California, México. We synonymize four subspecies circumscribed by the western clade and recognize a new species Arizona occidentalis to re-align the taxonomy with the phylogeographic structure. Most of the diversity within A. occidentalis occurs in California, with three major lineages corresponding separate desert biomes. We revise the conservation units within A. occidentalis to mirror these lineages and address concerns regarding habitat loss in transitional environments along the western edge of its range. This work underscores the importance of aligning taxonomy, evolutionary identity, and management units to design the most effective conservation strategies.

Molecular Phylogenetics and Evolution

Multistate models for estimation of survival and reproduction in the Grey-headed Albatross ( Thalassarche chrysostoma )

Reliable information on demography is necessary for conservation of albatrosses, the most threatened family of pelagic birds. Albatross survival has been estimated using mark-recapture data and the Cormack-Jolly-Seber (CJS) model. However, albatross exhibit skipped breeding, violating assumptions of the CJS model. Multistate modeling integrating unobservable states is a promising tool for such situations. We applied multistate models to data on Grey-headed Albatross ( Talassarche chrysostoma ) to evaluate model performance and describe demographic patterns. These included a multistate equivalent of the CJS model (MS-2), including successful and failed breeding states and ignoring temporary emigration, and three versions of a four-state multistate model that accounts for temporary emigration by integrating unobservable states: a model (MS-4) with one sample per breeding season, a robust design model (RDMS-4) with multiple samples per season and geographic closure within the season, and an open robust design model (ORDMS-4) with multiple samples per season and staggered entry and exit of animals within the season. Survival estimates from the MS-2 model were higher than those from the MS-4 model, which resulted in apparent percent relative bias averaging 2.2%. The ORDMS-4 model was more appropriate than the RDMS-4 model, given that staggered entry and exit occurred. Annual survival probability for Greyheaded Albatross at Marion Island was 0.951 ± 0.006 (SE), and the probability of skipped breeding in a subsequent year averaged 0.938 for successful and 0.163 for failed breeders. We recommend that multistate models with unobservable states, combined with robust-design sampling, be used in studies of species that exhibit temporary emigration.

The Auk