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USGS1984

MICROCHARACTERIZATION OF ARSENIC- AND SELENIUM-BEARING PYRITE IN UPPER FREEPORT COAL, INDIANA COUNTY, PENNSYLVANIA.

Optical and scanning electron microscope as well as electron and proton microprobe techniques have been used in a detailed investigation of the modes of occurrence of arsenic and selenium in pyrite in Upper Freeport coal from the Homer City area, Indiana County, Pennsylvania. Polished blocks were prepared from columnar samples of the coal bed to represent particular zones continuously from top to bottom. Initial selection of zones to be studied was based on chemical analysis of bench-channel samples. Microprobe data indicate that the highest concentrations of arsenic (as great as 1. 5 wt. %) are apparently in solid solution in pyrite within a limited stratigraphic interval of the coal bed. Smaller amounts of arsenic and selenium (concentrations up to approximately 0. 1 and 0. 2 wt. % respectively) were detected at isolated points within pyrite grains in various strata of the coal bed.

Scanning Electron Microscopy↗

USGS1987

APPLICATIONS OF CATHODOLUMINESCENCE OF QUARTZ AND FELDSPAR TO SEDIMENTARY PETROLOGY.

Cathodoluminescence (CL), the emission of visible light during electron bombardment, was first used in sandstone petrology in the mid-1960's. CL techniques are especially useful for determining the origin and source of quartz and feldspar, two of the most common constituents in clastic rocks. CL properties of both minerals are dependent on their temperature of crystallization, duration of cooling, and/or history of deformation. Detrital quartz and feldspar are typically derived from igneous and metamorphic sources and luminesce in the visible range whereas authigenic quartz and feldspar form at low temperatures and do not luminesce. Quantification of luminescent and non-luminescent quartz and feldspar with the scanning electron microscope, electron microprobe, or a commercial CL device can allow for the determination of origin, diagenesis, and source of clastic rocks when used in conjunction with field and other petrographic analyses.

Scanning Micros↗

USGS1992

Hydrothermal and metamorphic berthierine from the Kidd Creek volcanogenic massive sulfide deposit, Timmins, Ontario

Berthierine, a 7 A?? Fe-Al member of the serpentine group, occurs in the footwall stringer zone of the Archean Kidd Creek massive sulfide deposit, associated with quartz, muscovite, chlorite, pyrite, sphalerite, chalcopyrite, and local tourmaline, cassiterite, and halloysite. Petrographic and scanning electron microscopic (SEM) studies reveal different types of berthierine occurrences, including interlayers within the rims on deformed chlorite, intergrowths with muscovite and halloysite, and discrete coarse grains. This is the first reported occurrence of berthierine from volcanogenic massive sulfide deposits. Textural relations suggest that most of the berthierine formed as a primary hydrothermal mineral at relatively high temperatures (~350??C) in the footwall stringer zone, probably by the replacement of a pre-existing aluminous phase such as muscovite or chlorite. However, the intergrowth textures observed by SEM and TEM suggest that some of the berthierine originated by syn- or post-metamorphic replacement of chlorite. -from Authors

Canadian Mineralogist↗

USGS1996

Research-derived insights into surface geochemical hydrocarbon exploration

Research studies based on foreland basins (mainly in eastern Colorado) examined three surface geochemical exploration (SGE) methods as possible hydrocarbon (HC) exploration techniques. The first method, microbial soil surveying, has high potential as an exploration tool, especially hi development and enhanced recovery operations. Integrative adsorption, the second technique, is not effective as a quantitative SGE method because water, carbon dioxide, nitrous oxide, unsaturated hydrocarbons, and organic compounds are collected by the adsorbent (activated charcoal) much more strongly than covalently bonded microseeping Q-Cs thermogenic HCs. Qualitative comparisons (pattern recognition) of C8+ mass spectra cannot gauge HC gas microseepage that involves only the Q-Cs HCs. The third method, soil cakite surveying, also has no potential as an exploration tool. Soil calcite concentrations had patterns with pronounced areal contrasts, but these patterns had no geometric relationship to surface traces of established or potential production, that is, the patterns were random. Microscopic examination of thousands of soils revealed that soil calcite was an uncrystallized caliche coating soil particles. During its precipitation, caliche captures or occludes any gases, elements, or compounds in its immediate vicinity. Thus, increased signal intensity of some SGE methods should depend on increasing soil calcite concentrations. Analyses substantiate this hypothesis. Because soil calcite has no utility as a surface exploration tool, any surface method that depends on soil calcite has a diminished utility as an SGE tool. Isotopic analyses of soil calcites revealed carbonate carbon ??13C values of -4.0 to +2.07co (indicating a strong influence of atmospheric CO2) as opposed to expected values of-45 to -30%c if the carbonate carbon had originated from microbial oxidation of microseeping HC gases. These analyses confirm a surface origin for this soil calcite (caliche), which is not necessarily related to HC gas microseepage. This previously unappreciated pivotal role of caliche is hypothesized to contribute significantly to the poor and inconsistent results of some SGE methods.

AAPG Memoir↗

USGS2002

Control of strong ground motion of mining-induced earthquakes by the strength of the seismogenic rock mass

The shear stress ?? that can be sustained by the rock mass in the environs of a mining-induced earthquake controls the near-fault peak ground velocity v of that event according to v???0.25(??/G) ??, where ?? is the shear wave speed and G is the modulus of rigidity. To estimate ?? at mining depths, I review the results of four studies involving Witwatersrand tremors that relate to the bulk shear strength. The first and most general analysis uses the common assumptions that the seismogenic crust is pervasively faulted, has hydrostatic pore pressure before mining, and an extensional stress state that is close to failure. Mining operations reduce the pore pressure to zero within the mine and redistribute the stresses such that, in localized regions, the state of stress is again at the point of failure. Laboratory friction experiments can be used to estimate ?? in the zero-pore-pressure regime. Second, model calculations of states of stress in the vicinity of milling at about 3 km depth indicated the shear stress available to cause faulting near the centre of a distribution of induced earthquakes. Third, laboratory experiments combined with microscopic analyses of fault gouge from the rupture zone of a mining-induced event provided an estimate of the average shear stress acting on the fault to cause this earthquake at a depth of 2 km. Fourth, moment tensors determined for mining- induced earthquakes usually show substantial implosive components, from which it is straightforward to estimate ??. These four different analyses yield estimates of ?? that fall in the range 30 to 61 MPa which implies that near-fault particle velocities could he as high as about 1.5 m/s. To the extent that the causative fault ruptures previously intact rock, both ?? and v, in localized regions, could be several times higher than 61 MPa and 1.5 m/s.

Journal of the South African Institute of Mining a↗

USGS2003

Athena Mars rover science investigation

Each Mars Exploration Rover carries an integrated suite of scientific instruments and tools called the Athena science payload. The primary objective of the Athena science investigation is to explore two sites on the Martian surface where water may once have been present, and to assess past environmental conditions at those sites and their suitability for life. The remote sensing portion of the payload uses a mast called the Pancam Mast Assembly (PMA) that provides pointing for two instruments: the Panoramic Camera (Pancam), and the Miniature Thermal Emission Spectrometer (Mini-TES). Pancam provides high-resolution, color, stereo imaging, while Mini-TES provides spectral cubes at mid-infrared wavelengths. For in-situ study, a five degree-of-freedom arm called the Instrument Deployment Device (IDD) carries four more tools: a Microscopic Imager (MI) for close-up imaging, an Alpha Particle X-Ray Spectrometer (APXS) for elemental chemistry, a Mo??ssbauer Spectrometer (MB) for the mineralogy of Fe-bearing materials, and a Rock Abrasion Tool (RAT) for removing dusty and weathered surfaces and exposing fresh rock underneath. The payload also includes magnets that allow the instruments to study the composition of magnetic Martian materials. All of the Athena instruments have undergone extensive calibration, both individually and using a set of geologic reference materials that are being measured with all the instruments. Using a MER-like rover and payload in a number of field settings, we have devised operations processes that will enable us to use the MER rovers to formulate and test scientific hypotheses concerning past environmental conditions and habitability at the landing sites. Copyright 2003 by the American Geophysical Union.

Journal of Geophysical Research E: Planets↗

USGS2003

Mycorrhizal colonization across hydrologic gradients in restored and reference freshwater wetlands

Arbuscular mycorrhizae, which are plant root-fungal symbioses, are common associates of vascular plants. Such relationships, however, are thought to be rare in wetland plant roots, although several recent studies suggest that arbuscular mycorrhizae may be important in wetland ecosystems. Our objectives were to determine (1) the level of arbuscular mycorrhizal colonization of plant roots in three freshwater marshes and (2) the effect of restoration status, hydrologic zone, and plant species identity on mycorrhizal colonization. We quantified the percentage of plant roots colonized by mycorrhizal fungi in one reference and two restored freshwater marshes in northern Indiana, USA during summer 1999. Roots were collected from soil cores taken around dominant plant species present in each of three hydrologic zones and then stained for microscopic examination of mycorrhizal colonization. Mycorrhizae were present in each wetland, in all hydrologic zones and in all sampled plants, including Carex and Scirpus species previously thought to be non-mycorrhizal. Both restored and reference wetlands had moderate levels of mycorrhizal colonization, but no clear trends in colonization were seen with hydrologic zone, which has been hypothesized to regulate the formation of mycorrhizae in wetlands. Mycorrhizal colonization levels in the roots of individual species ranged from 3 to 90% and were particularly large in members of the Poaceae (grass) family. Our results suggest that arbuscular mycorrhizae may be widely distributed across plant species and hydrologic zones in both restored and reference freshwater marshes. Thus, future research should examine the functional role of mycorrhizal fungi in freshwater wetlands. ?? 2003, The Society of Wetland Scientists.

Wetlands↗

USGS2004

Effects of housing density and cage floor space on C57BL/6J mice

The Guide for the Care and Use of Laboratory Animals (the Guide) is widely accepted as the housing standard by most Institutional Animal Care and Use Committees. The recommendations are based on best professional judgment rather than experimental data. Current efforts are directed toward replacing these guidelines with data-driven, species-appropriate standards. Our studies were undertaken to determine the optimum housing density for C57BL/6J mice, the most commonly used inbred mouse strain. Four-week-old mice were housed for 8 weeks at four densities (the recommended ???12 in2 [ca. 77.4 cm 2]/mouse down to 5.6 in2 [ca. 36.1 cm2]/mouse) in three cage types with various amounts of floor space. Housing density did not affect a variety of physiologic parameters but did affect certain micro-environmental parameters, although these remained within accepted ranges. A second study was undertaken housing C57BL/6J mice with as little as 3.2 in2/mouse (ca. 20.6 cm2). The major effect was elevated ammonia concentrations that exceeded limits acceptable in the workplace at increased housing densities; however, the nasal passages and eyeballs of the mice remained microscopically normal. On the basis of these results, we conclude that C57BL/6J mice as large as 29 g may be housed with 5.6 in2 of floor space per mouse. This area is approximately half the floor space recommended in the Guide. The role of the Guide is to ensure that laboratory animals are well treated and housed in a species-appropriate manner. Our data suggest that current policies could be altered in order to provide the optimal habitation conditions matched to this species' social needs. Copyright 2004 by the American Association for Laboratory Animal Science.

Comparative Medicine↗

USGS2006

Impact of quaternary climate on seepage at Yucca Mountain, Nevada

Uranium-series ages, oxygen-isotopic compositions, and uranium contents were determined in outer growth layers of opal and calcitefrom 0.5- to 3-centimeter-thick mineral coatings hosted by lithophysal cavities in the unsaturated zone at Yucca Mountain, Nevada, the proposed site of a permanent repository for high-level radioactive waste. Micrometer-scale growth layering in the minerals was imaged using a cathodoluminescence detector on a scanning electron microscope. Determinations of the chemistry, ages, and delta oxygen-18 (??18O) values of the growth layers were conducted by electron microprobe analysis and secondary ion mass spectrometry techniques at spatial resolutions of 2 to about 20 micrometers (??m) and 25 to 40 ??m, respectively. Growth rates for the last 300 thousand years (k.y.) calculated from about 300 new high-resolution uranium-series ages range from approximately 0.5 to 1.5 ??m/k.y. for 1- to 3-centimeter-thick coatings, whereas coatings less than about 1-centimeter-thick have growth rates less than 0.5 ??m/k.y. At the depth of the proposed repository, correlations of uranium concentration and ??18O values with regional climate records indicate that unsaturated zone percolation and seepage water chemistries have responded to changes in climate during the last several hundred thousand years.

Conference Paper↗

USGS1989

Diagenesis of hydrocarbon-bearing rocks in the Middle Ordovician Simpson Group, southeastern Anadarko Basin, Oklahoma

Quartzarenites and subarkoses in the Middle Ordovician Simpson Group in the Gulf Costello No. 1 and Sunray-DX Parker No. 1 Mazur wells, southeastern Anadarko basin, have undergone a complex diagenetic and petroleum-migration history. During early burial, petroleum migrated locally through sandstones; patches of bitumen in calcite and bitumen-lined quartz overgrowths containing oil-bearing inclusions reflect the introduction of petroleum-bearing fluids at shallow depths. Stable-isotope data reveal that early calcite precipitated at near-surface temperatures from fluids dominated by marine carbon. At moderate to deep burial, calcite dissolution, followed by ferroan-dolomite and clay-mineral precipitation, occurred at about the same time as the rocks reached levels of thermal maturity sufficient for the generation of hydrocarbons. Maximum paleotemperatures during deep burial are estimated from maturation models to have reached 250°F in the Costello well and 300°F in the Mazur well. Maturation-derived temperatures in the Costello well are consistent with preliminary homogenization temperatures (210-250°F) for oil inclusions along microscopic healed fractures that formed during deep burial, thus supporting an Early to Middle Pennsylvanian timing for the generation and migration of late-stage hydrocarbons. The early petroleum phase, emplaced while the rocks were at shallow burial depths, migrated from mature source rocks deeper in the basin.

Oklahoma↗

USGS2013

Epidote from the Zard Mountains, Kharan, Balochistan, Pakistan

The authors received two unusual crystals of epidote from Rock Currier, Jewel Tunnel Imports, in 2012. The mineral specimens were collected at Zard Mountain (Zard Koh), in the central part of the Ruskoh Mountains (Rusk Koh), west of Kharan, Balochistan, Pakistan (written communication, Rock Currier, 2013). The epidote locality was most likely discovered in 2010. These epidote crystals were unusual in both form and composition. The large crystals were flat tabular and pseudohexagonal in shape which is an uncommon crystal form for a monoclinic mineral (fig. 1). Other specimens from the same locality have been described as pseudo-octahedral in shape. The two crystals range in size from 5.5 to 6.5 centimeters (2.2 to 2.6 inches) and are slightly magnetic. The epidote crystals have a core matrix that resembles a weathered igneous rock. Some micro brown- to reddish-titanite crystals were observed under a binocular microscope on the surface and core areas of the crystals (figs. 2 and 3). Other minerals observed in the core areas include feldspar, biotite, and quartz. The crystals display evidence of cluster-growth with points of attachment to other crystals. The epidotes were most likely collected in pockets of a weathered igneous-skarn deposit.

Balochistan, Zard Mountains↗

USGS2014

Mercury in the national parks

One thing is certain: Even for trained researchers, predicting mercury’s behavior in the environment is challenging. Fundamentally it is one of 98 naturally occurring elements, with natural sources, such as volcanoes, and concentrated ore deposits, such as cinnabar. Yet there are also human-caused sources, such as emissions from both coal-burning power plants and mining operations for gold and silver. There are elemental forms, inorganic or organic forms, reactive and unreactive species. Mercury is emitted, then deposited, then re-emitted—thus earning its mercurial reputation. Most importantly, however, it is ultimately transferred into food chains through processes fueled by tiny microscopic creatures: bacteria.

The George Wright Forum↗

USGS2014

Distribution and prevalence of knemidokoptic mange in Hawai`i `Amakihi on the island of Hawaii

Knemidokoptic mange was first observed on two Hawai‘i ‘Amakihi (Hemignathus virens) mist netted in Manuka Natural Area Reserve (NAR) on the Island of Hawai‘i in June 2007. Microscopic examination of skin scrapings from lesions of the infested individuals revealed the scaley-leg mite, Knemidokoptes jamaicensis. Continued surveillance at Manuka NAR (2007-2009) documented a 24% (15/63) prevalence of mange among Hawai‘i ‘Amakihi distributed from coastal habitat to 1,500 m above sea level (asl). From 2012-2014, we conducted an island-wide survey of wild passerine birds from several leeward sites (Manuka NAR, Kahuku Unit of Hawai‘i Volcanoes National Park (HAVO), Pu‘u Wa‘awa‘a Forest Bird Sanctuary, and Kipahoehoe NAR) and windward sites (Hakalau Forest National Wildlife Refuge, ‘Ᾱinahou Ranch of HAVO, Malama Ki Forest Reserve, and Keauohana Forest Reserve) to determine the current distribution and host range of knemidokoptic mange. We also determined the prevalence of malaria in Hawai‘i ‘Amakihi populations where mange was present and treated a subset of infested Hawai‘i ‘Amakihi mange with a single, topical dose of moxidectin. We mist netted and examined a total of 1,734 passerines, including 738 Hawai‘i ‘Amakihi. Mange was present in Hawai‘i ‘Amakihi at Manuka NAR (595 and 305 m asl), Kahuku Ranch Unit of HAVO (Glover site: 1,201 m asl and Kipuka Akala site: 1,532 m asl), Malama Ki Forest Reserve and Keauohana Forest Reserve (293 m asl). No other passerine birds (n = 995) were infected. Mange prevalence ranged from a high of 69% (40/58) in Keauohana Forest Reserve to a low of 2% (1/65) in the Kahuku Ranch Unit of HAVO (Kipuka Akala). At Manuka NAR prevalence had decreased from 26% in 2010 to 10% (7/81) in 2012–2014. We found no significant relationship between the prevalence of mange and the prevalence of avian malaria in mesic habitats at Manuka NAR (P = 0.59 (FET, n = 81)), but there was a significant association between the prevalence of mange and the prevalence of malaria in lowland wet forests in Puna Forest Reserves (P < 0.01 (FET, n = 72)). This apparent association may be a reflection of the high prevalence of malaria (>80%) in these areas. There was no difference in the frequency of recapture of birds that were infested versus un-infested at first capture at our long-term sites (Manuka NAR and Puna sites) (χ2(1, n = 227) = 1.51, P = 0.22, but when all sites with mange present were pooled, there was a significant difference in the frequency of recaptures between infested and un-infested birds (χ2(1, n = 424) = 7.13, P = 0.01). There was a significant association between parasitemia level (per 10,000 RBCs) and the ranked stage of mange present in infested individuals. We treated 24 Hawai‘i ‘Amakihi with moxidectin and upon recapture (n = 2), found a reduction in both the size and stage of mange lesions, such that a single dose, topical treatment of moxidectin appears to be an effective treatment for knemidokoptic mange in wild populations. Our results suggest that knemidokoptic mange is currently limited to Hawai‘i ‘Amakihi and prevalent in low elevation sites on both the windward and leeward sides of the island.

Hawaii↗

USGS2011

The efficacy of mass-marking channel catfish fingerlings by immersion in oxytetracycline

Oxytetracycline (OTC) has been extensively used for marking a variety of fish species, but has never been successfully used to mark channel catfish Ictalurus punctatus. Channel catfish fingerlings (~ 25 mm TL) obtained from the Oklahoma Department of Wildlife Conservation at Byron Fish Hatchery were kept in Living Streams (791 to 1,018 L) equipped with recirculation units. Marking trials consisted of immersing channel catfish in one of three concentrations (250, 450, and 700 mg/L) OTC hydrochloride [HCl] for 6 hours. Samples of channel catfish were obtained from each group at 1-week and 4-week postimmersion. Lapilli otoliths and pectoral spines were removed to assess mark presence with an epi-fluorescent compound microscope. After one week, no marks were detected on pectoral spines for all treatments, mark detection on otoliths depended on concentration, but never exceeded 43% (700 mg/L). After four weeks, all otoliths and pectoral spines were determined marked for 700 mg/L OTC, 20% for fish immersed in 450 mg/L OTC, and 0% were marked after four weeks at the 250 mg/L OTC. Results show, channel catfish fingerlings can be successfully marked with immersion in OTC at 700 mg/L for at least 6 hours.

Proceedings of the Oklahoma Academy of Science↗

USGS1975

Experimental abrasion of detrital gold

The physical breakdown and abrasion rates of gold were studied using a tumbler to simulate natural high-energy environments. The gold fragments were tumbled for periods ranging from 30 to 240 h with different combinations of sand, cobbles, and water at velocities of 0.5 and 2.0 mi/h (0.85 and 3.22 km/h). With sand and gravel, the common bedload of the rivers that deposited the gold-bearing Tertiary sedimentary rocks of the Sierra Nevada, gold is abraded at rates of 0.015 to 0.007 percent (by weight) per hour of travel (at 0.5 mi/h or 0.845 km/h). Cobbles, rather than sand, are responsible for most of the physical changes and abrasion of the gold. Ten gold fragments tumbled for 120 h with cobbles and water (no sand) were broken down to 68 recoverable fragments and lost about 25 percent of their weight to particles smaller than could be recovered using conventional panning techniques. Gold tumbled for 120 h with sand and water lost less than 1 percent of its weight. Gold was abraded faster by wet sand than by dry sand. Velocity appears to be more important as a factor in abrasion of gold than travel distance a fourfold increase in velocity produced a tenfold increase in hourly abrasion rates of gold. Scanning electron microscope examination of the gold fragments after the tumbling experiments revealed differences in surface texture between fragments tumbled with (1) sand, (2) sand and cobbles, and (3) cobbles only.

Journal of Research of the U.S. Geological Survey↗

USGS2016

Accretionary lapilli: what’s holding them together?

Accretionary lapilli from Tagus cone, Isla Isabela, Galápagos were analyzed using scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDS) techniques. Our main findings are (1) the lapilli formed and hardened in a few minutes while still aloft in the dispersing eruption column. (2) Palagonite rinds developed first on the basaltic glass clasts, and subsequently crystallized (3) The crystallization products contain submicron lamellar crystals of a clay (probably smectite) on the surfaces of basaltic glass clasts and (4) The interlocking of these lamellar clays from adjacent clasts binds and cements them together to form the accretionary lapillus. We argue that palagonite and possibly clay formation occur primarily in the presence of hot water vapor.

Conference Paper↗

USGS2006

What is causing the phytoplankton increase in San Francisco Bay?

The largest living component of San Francisco Bay is the phytoplankton, a suspension of microscopic cells that convert sunlight energy into new living biomass through the same process of photosynthesis used by land plants. This primary production is the ultimate source of food for clams, zooplankton, crabs, sardines, halibut, sturgeon, diving ducks, pelicans, and harbor seals. From measurements made in 1980, we estimated that phytoplankton primary production in San Francisco Bay was about 200,000 tons of organic carbon per year (Jassby et al. 1993). This is equivalent to producing the biomass of 5500 adult humpback whales, or the calories to feed 1.8 million people. These numbers may seem large, but primary production in San Francisco Bay is low compared to many other nutrient-enriched estuaries.

California↗

USGS1987

Standardization of stain used for diagnosing erythrocytic inclusion body syndrome (EIBS)

Erythrocytic inclusion body syndrome (EIBS), a viral erythrocytic necrosis (VEN)-like disease, has been observed in several areas in the Northwest. This virus disease is clinically diagnosed by microscopic examination of blood smears for intracytoplasmic erythrocytic inclusion bodies. Fish biologists involved in EIBS diagnostic work have been using several types of hematological stains. It became apparent that standardization of the staining procedure was needed. Comparative tests were conducted on blood smears and kidney imprints with the following commonly used blood stains: (1) Leishman-Giesma, (2) Pinacyanol chloride, (3) Powell 's Giemsa, (4) Harleco's Giemsa, (5) Diff Quik differential stain, (6) Wright's. Pinacyanol chloride stain was found to be the most consistent. The following staining procedure is recommended.

Fish Health Newsletter↗