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Application of ground-truth for classification and quantification of bird movements on migratory bird habitat initiative sites in southwest Louisiana: final report

This project was initiated to assess migrating and wintering bird use of lands enrolled in the Natural Resources Conservation Service’s (NRCS) Migratory Bird Habitat Initiative (MBHI). The MBHI program was developed in response to the Deepwater Horizon oil spill in 2010, with the goal of improving/creating habitat for waterbirds affected by the spill. In collaboration with the University of Delaware (UDEL), we used weather surveillance radar data (Sieges 2014), portable marine radar data, thermal infrared images, and visual observations to assess bird use of MBHI easements. Migrating and wintering birds routinely make synchronous flights near dusk (e.g., departure during migration, feeding flights during winter). Weather radars readily detect birds at the onset of these flights and have proven to be useful remote sensing tools for assessing bird-habitat relations during migration and determining the response of wintering waterfowl to wetland restoration (e.g., Wetlands Reserve Program lands). However, ground-truthing is required to identify radar echoes to species or species group. We designed a field study to ground-truth a larger-scale, weather radar assessment of bird use of MBHI sites in southwest Louisiana. We examined seasonal bird use of MBHI fields in fall, winter, and spring of 2011-2012. To assess diurnal use, we conducted total area surveys of MBHI sites in the afternoon, collecting data on bird species composition, abundance, behavior, and habitat use. In the evenings, we quantified bird activity at the MBHI easements and described flight behavior (i.e., birds landing in, departing from, circling, or flying over the MBHI tract). Our field sampling captured the onset of evening flights and spanned the period of collection of the weather radar data analyzed. Pre- and post-dusk surveys were conducted using a portable radar system and a thermal infrared camera. Landbirds, shorebirds, and wading birds were commonly found on MBHI fields during diurnal surveys in the fall. Ducks (breeding and early migrating species) were also detected on diurnal surveys, but were less abundant than the previously mentioned taxa. Wading birds were the most abundant taxa observed during evening surveys up to 5 min before dusk when their numbers declined and duck densities increased. Ducks accounted for 64.0% of all birds detected from 0-5 min before dusk. Most ducks observed at that time were flyovers (71.4%), but circling (9.2%), departing (12.1%), and landing birds (7.4%) were also detected. In fall, the portable radar system detected two peaks in bird movement: one shortly before sunset and a second shortly after dusk. The later movement began just before dusk, peaked approximately 9 min after dusk, and concluded within 20 min after dusk. The flight headings of birds changed in relation to time from dusk. In general, the majority of targets flew towards the southwest before dusk and towards the northeast after dusk. The change in flight direction pre- and post-dusk may be related to movements dominated by migratory versus local flight. In winter, ducks, shorebirds, wading birds, and landbirds were the most abundant taxa in diurnal surveys. Geese were abundant at times, but their frequency of occurrence and densities were highly variable. The majority of ducks, shorebirds, and wading birds were observed feeding in MBHI fields. Landbirds and geese were more commonly seen resting. Overwintering ducks and geese dominated the movements near dusk (95.9% of all birds ≤ 5 min pre-dusk). Ducks were more frequently observed landing in (40.8%) and flying over (33.5%) MBHI fields while geese were mainly observed circling (54.7%) and flying over (38.9%) sites. Most of the shorebirds detected < 5 min before dusk (74.6% of all shorebirds) were departing the MBHI fields. Portable radar and thermal infrared camera data indicate that large northeastward movements of waterfowl (99.9% of birds identified to taxa) occurred after dusk (~10 min post-dusk). Most birds observed on radar during this peak were flyovers and did not use the MBHI fields (78.9%); however, birds were detected landing in (10.9%) and departing from (2.9%) MBHI fields. The post-dusk movements may have been waterfowl feeding flights that routinely occur in southwest Louisiana between roost sites in coastal marsh and foraging sites in agricultural fields to the north. After the conclusion of these movements ca. 30 min post-dusk, portable radar data showed little activity through the night until approximately 0.5 to 1.5 hr pre-dawn. Radar data within 30 min pre-dawn indicate that most birds departed MBHI fields on flight headings toward the southwest. The pre-dawn movements were likely waterfowl departing from their foraging sites and returning to roosting areas in coastal marshes to the south. Shorebirds, ducks, and wading birds were the most abundant taxa during diurnal surveys of MBHI fields in spring, and the majority of individuals were observed actively foraging rather than resting. Breeding, overwintering, and transient migrant species were all detected on MBHI fields. Near dusk, the majority of birds in flight were ducks (67.7% of all birds) that were flying over (38.2%), departing from (34.2%), or landing in (22.9%) MBHI fields. These results contrast with our winter observations when 40.8% of ducks landed in MBHI fields and 9.1% departed from fields. Portable radar and thermal camera data documented a peak in bird movements shortly after dusk, however, the peak was of lower magnitude than observed in the winter. Thermal camera data identified the birds as mostly shorebirds (57.3%) and waterfowl (40.4%). Flight headings were more variable than winter and lacked an undirectional flow. After the post-dusk movement had concluded, bird activity remained low throughout the night until approximately 30 min before dawn when a small peck in activity was observed. Flight headings during the pre-dawn were variable and multidirectional. We compared bird abundance data collected by each of our three sampling techniques (portable radar, thermal infrared camera, and direct visual observation) for the 45-min observation period immediately preceding dusk; the period when all three survey methods were used simultaneously. Abundance data from the three methods were significantly correlated at P &le; 0.05. We documented diurnal and nocturnal bird use of MBHI fields. Most observations near dusk in winter, when weather radar data were sampled, were of ducks and geese, and in spring, shorebirds and ducks. Our winter observations show large synchronous movements of waterfowl occurring near dusk. These birds were moving to the NE and feeding in agricultural fields at night. Portable radar data suggest that birds stay in these fields through the night and make return flights near dawn.

Louisiana↗

Development of a national, dynamic reservoir-sedimentation database

The importance of dependable, long-term water supplies, coupled with the need to quantify rates of capacity loss of the Nation’s re servoirs due to sediment deposition, were the most compelling reasons for developing the REServoir- SEDimentation survey information (RESSED) database and website. Created under the auspices of the Advisory Committee on Water Information’s Subcommittee on Sedimenta ion by the U.S. Geological Survey and the Natural Resources Conservation Service, the RESSED database is the most comprehensive compilation of data from reservoir bathymetric and dry-basin surveys in the United States. As of March 2010, the database, which contains data compiled on the 1950s vintage Soil Conservation Service’s Form SCS-34 data sheets, contained results from 6,616 surveys on 1,823 reservoirs in the United States and two surveys on one reservoir in Puerto Rico. The data span the period 1755–1997, with 95 percent of the surveys performed from 1930–1990. The reservoir surface areas range from sub-hectare-scale farm ponds to 658 km 2 Lake Powell. The data in the RESSED database can be useful for a number of purposes, including calculating changes in reservoir-storage characteristics, quantifying sediment budgets, and estimating erosion rates in a reservoir’s watershed. The March 2010 version of the RESSED database has a number of deficiencies, including a cryptic and out-of-date database architecture; some geospatial inaccuracies (although most have been corrected); other data errors; an inability to store all data in a readily retrievable manner; and an inability to store all data types that currently exist. Perhaps most importantly, the March 2010 version of RESSED database provides no publicly available means to submit new data and corrections to existing data. To address these and other deficiencies, the Subcommittee on Sedimentation, through the U.S. Geological Survey and the U.S. Army Corps of Engineers, began a collaborative project in November 2009 to modernize the RESSED database architecture; provide public online input capability; and produce online reports. The ultimate goal of the Subcommittee on Sedimentation is to build a comprehensive, quality-assured database describing capacity changes over time for the largest suite of the Nation’s reservoirs.

Conference Paper↗

Recent trends of desert tortoise populations in the Mojave Desert

The desert tortoise, Gopherus agassizii (Cooper), was listed as threatened in the Mojave Desert in 1990. Important factors for the listing were severe recent mortality in tortoise populations and a general decline throughout this century. Recent trends in tortoise populations were examined by plotting total captures of adult and juvenile tortoises from 2.6-km 2 study plots, rather than by mark-and-recapture population estimates. Changes in relative abundance of tortoises were greatest among large tortoises in the western Mojave Desert, which may reflect high levels of human disturbance, and among small tortoises, which may reflect either lower ability of searches to detect small tortoises or greater mortality of tortoises during drought conditions in 1986-90, or both factors. Further collection of data on population trends is needed, particularly in years with higher-than-average precipitation. Retention of the threatened status of the tortoise is a conservative strategy for the conservation of natural resources but should be reassessed when additional data are available.

California, Nevada↗

Blackbrush ( Coleogyne ramosissima Torr. ): State of our knowledge and future challenges

Covering 130,000 square miles and a wide range of elevations from desert to alpine in Arizona, Utah, Colorado, and New Mexico, the Colorado Plateau has long fascinated researchers. The Colorado Plateau VI provides readers with a plethora of updates and insights into land conservation and management questions currently surrounding the region. The Colorado Plateau VI’s contributors show how new technologies for monitoring, spatial analysis, restoration, and collaboration improve our understanding, management, and conservation of outcomes at the appropriate landscape scale for the Colorado Plateau. The volume’s chapters fall into five major themes: monitoring as a key tool for addressing management challenges, restoration approaches to improving ecosystem condition and function, collaboration and organizational innovations to achieve conservation and management objectives, landscape-scale approaches to understanding, and managing key species and ecological communities. Focusing on the integration of science into resource management issues over the Colorado Plateau, this volume includes contributions from dozens of leading scholars of the region. The Colorado Plateau VI proves a valuable resource to all interested in the conservation management, natural history, and cultural biological resources of the Colorado Plateau.

Arizona, Colorado, New Mexico, Utah↗

Trouble in the aquatic world: How wildlife professionals are battling amphibian declines

A parasitic fungus, similar to the one that caused the extinction of numerous tropical frog and toad species, is killing salamanders in Europe. Scientists first identified the fungus, Batrachochytrium salamandrivorans, in 2013 as the culprit behind the death of fire salamanders ( Salamandr a salamandra ) in the Netherlands ( Martel et al. 2013 ) and are now exploring its potential impact to other species. Although the fungus, which kills the amphibians by infecting their skin, has not yet spread to the United States, researchers believe it’s only a matter of time before it does and, when that happens, the impact on salamander populations could be devastating ( Martel et al. 2014 ). Reports of worldwide declines of amphibians began a quarter of a century ago ( Blaustein & Wake 1990 ). Globally, some amphibian population declines occurred in the late 1950s and early 1960s, and declining trends continued in North America ( Houlahan et al. 2000 ). In the earlier years, population declines were attributed primarily to overharvest due to unregulated supply of species such as the northern leopard frog ( Lithobate s pipiens ) for educational use ( Dodd 2013 ). In later years, however, causes of declines were less evident. In 1989, herpetologists at the First World Congress of Herpetology traded alarming stories of losses across continents and in seemingly protected landscapes, making it clear that amphibian population declines were a “global phenomenon.” In response to these reports, in 1991, the International Union for Conservation of Nature (IUCN) established the Declining Amphibian Populations Task Force to better understand the scale and scope of global amphibian declines. Unfortunately, the absence of long-term monitoring data and targeted studies made it difficult for the task force to compile information. Today, according to AmphibiaWeb.org, there are 7,342 amphibian species in the world — double the number since the first alerts of declines — making the situation appear deceptively less dire. In fact, our understanding of genetic diversity significantly raises the stakes, and we are at risk of losing far more species than we believed only a few years ago. According to the IUCN, amphibians now lead the list of vertebrate taxa affected by the larger “biodiversity crisis” and sixth major mass- extinction event on Earth ( Keith et al. 2014 , Wake and Vredenburg 2008 ).

The Wildlife Professional↗

The biogeography of threatened insular iguanas and opportunities for invasive vertebrate management

Iguanas are a particularly threatened group of reptiles, with 61% of species at risk of extinction. Primary threats to iguanas include habitat loss, direct and indirect impacts by invasive vertebrates, overexploitation, and human disturbance. As conspicuous, charismatic vertebrates, iguanas also represent excellent flagships for biodiversity conservation. To assist planning for invasive vertebrate management and thus benefit threatened iguana recovery, we identified all islands with known extant or extirpated populations of Critically Endangered and Endangered insular iguana taxa as recognized by the International Union for Conservation of Nature (IUCN) Red List of Threatened Species. For each island, we determined total area, sovereignty, the presence of invasive alien vertebrates, and human population. For the 23 taxa of threatened insular iguanas we identified 230 populations, of which iguanas were extant on 185 islands and extirpated from 45 islands. Twenty-one iguana taxa (91% of all threatened insular iguana taxa) occurred on at least one island with invasive vertebrates present; 16 taxa had 100% of their population(s) on islands with invasive vertebrates present. Rodents, cats, ungulates, and dogs were the most common invasive vertebrates. We discuss biosecurity, eradication, and control of invasive vertebrates to benefit iguana recovery: (1) on islands already free of invasive vertebrates; (2) on islands with high iguana endemicity; and (3) for species and subspecies with small total populations occurring across multiple small islands. Our analyses provide an important first step toward understanding how invasive vertebrate management can be planned effectively to benefit threatened insular iguanas.

Book chapter↗

Constraining parameter uncertainty in modeling debris-flow initiation during the September 2013 Colorado Front Range storm

The occurrence of debris flows during the September 2013 northern Colorado floods took the emergency management community by surprise. The September 2013 debris flows in the Colorado Front Range initiated from shallow landslides in colluvium. Most occurred on south- and east-facing slopes on the walls of steep canyons in crystalline rocks and on sedimentary hogbacks. Previous studies showed that most debris flows occurred in areas of high storm-total rainfall and that strength added by tree roots accounts for the low number of landslides in densely forested areas. Given the lack of rainfall thresholds for debris flow occurrence in northern Colorado, we want to parameterize a numerical model to assess potential for debris flows in advance of heavy rainfall. Natural Resources Conservation Service (NRCS) soil mapping of the area, supplemented by laboratory testing and field measurements, indicates that soil textures and hydraulic properties of landslide source materials vary considerably over the study area. As a step toward modeling storm response, available soil and geologic mapping have been interpreted to define zones of relatively homogeneous properties. A new, simplified modeling approach for evaluating model input parameters in the context of slope and depth of observed debris flow source areas and recorded debris-flow inducing rainfall helps narrow the range of possible parameters to those most likely to produce model results consistent with observed debris flow initiation. Initial results have narrowed the strength parameters to about one third of possible combinations of cohesion and internal friction angle and narrowed hydraulic conductivity to a range spanning slightly more than one order of magnitude.

Colorado↗

Scientific Framework for resilience research on the Upper Mississippi River System

The goal of this research framework is to outline research that would continue to improve our understanding of ecological resilience of the Upper Mississippi River System (UMRS) and inform management of the system for health and resilience. We provide a broad overview of recently completed and ongoing work that has been funded as a part of the UMRS Ecological Resilience Assessment and related efforts to provide context for how proposed research questions build upon our current knowledge and may inform restoration planning and design. We describe two primary objectives within this research framework - the first objective is to investigate hypothesized drivers and feedbacks related to specified resilience, and the second is to expand our utility of general resilience indicators through further development and evaluation. The intended audience for this framework is the Upper Mississippi River Restoration Program (UMRR). The UMRR partnership is made up of natural resource agencies from five states (Illinois, Iowa, Minnesota, Missouri and Wisconsin) and five federal agencies (U.S. Army Corps of Engineers, U.S. Environmental Protection Agency, U.S. Fish and Wildlife Service, U.S. Geological Survey, and USDA Natural Resources Conservation Service).

Illinois, Iowa, Minnesota, Missouri, Wisconsin↗

Global status of trout and char: Conservation challenges in the twenty-first century

Freshwater ecosystems are among the most threatened ecosystems in the world (Richter et al. 1997; Strayer and Dudgeon 2010), and freshwater fishes may now be the most threatened group of vertebrates (Ricciardi and Rasmussen 1999; Vorosmarty et al. 2010; Darwall and Freyhof 2016). Of the 7,300 freshwater fish species globally assessed by the International Union for Conservation of Nature (IUCN, www.iucnredlist.org) in 2013, nearly one of every three species was threatened with extinction (Darwall and Freyhof 2016). Growing pressures from a multitude of direct and indirect human stressors (e.g., habitat loss and degradation, pollution, invasive species, overexploitation, diversion or alteration of biological flows, climate change, and others) threaten the persistence of many freshwater fish species and entire aquatic communities around the globe (Limburg et al. 2011). This pattern is particularly true for salmonid fishes (family Salmonidae, subfamily Salmoninae, belonging to the genera Oncorhynchus, Salvelinus, Salmo, Hucho, Parahucho, Brachymystax, and Salvethymus). Salmonids are globally-distributed, coldwater taxa with life-cycles restricted entirely to freshwater ecosystems (typically referred to as trout and char), but also Atlantic and Pacific salmon with more complex anadromous life-histories.

Book chapter↗

Standardized guide to the examination and necropsy of the horseshoe crab using Limulus polyphemus as Limulidae prototype

The Atlantic, or American, horseshoe crab (L imulus polyphemus ) has existed largely unchanged for over 100 million years. Millions of individuals are commonly observed ashore in spring and summer months during spawning events along the entire North American coastline expanding from the East to the Gulf coasts of the United States and Mexico. Other species can be found in the Indian and Pacific Ocean. The massive deposit of eggs in nearshore sand provides a critical source of food for endangered migrating birds, especially the Red Knot ( Calidruis canutus rufa ) in the Delaware Bay. Horseshoe crabs are also an important component of the sea turtle diet. In addition to the ecological importance, horseshoe crabs are used commercially for bait in eel and conch fisheries and for biomedical purposes in the production of Limulus Amebocyte Lysate (LAL) to detect bacterial toxins in injectable drugs and implantable devices. Commercial demands have led to population declines in some regions. Fisheries are regulated by state and the current International Union for Conservation of Nature (IUCN) listing for L. polyphemus is vulnerable. A small number of individuals are housed in public aquaria for educational purposes. With growing interest in animal welfare, the health and stability of populations, and potential stressors that can contribute to decline , it is important to have clear and detailed descriptions of horseshoe crab anatomy and necropsy techniques. The purpose of this guide is to illustrate the normal anatomy and the step-by-step technique for dissection of horseshoe crabs. The contents are largely excerpts of the master’s thesis of artist, Katie (Bergdale) Roorda, which was based on photographs from C. Meteyer documenting the sequence and procedure used for necropsy dissection.

Cooperator Report↗

Potomac tributary report: A summary of trends in tidal water quality and associated factors

The Potomac Tributary Report summarizes change over time in a suite of monitored tidal water quality parameters and associated potential drivers of those trends for the time period 1985 – 2018, and provides a brief description of the current state of knowledge explaining these observed changes. Water quality parameters described include surface total nitrogen (TN), surface total phosphorus (TP), spring and summer surface chlorophyll a, summer bottom dissolved oxygen (DO) concentrations, and Secchi disk depth (a measure of water clarity). Results for annual surface water temperature, bottom TP, bottom TN, surface ortho-phosphate (PO4), surface dissolved inorganic nitrogen (DIN), surface total suspended solids (TSS), and summer surface DO concentrations are provided in an Appendix. Drivers discussed include physiographic watershed characteristics, changes in N, P, and sediment loads from the watershed to tidal waters, expected effects of changing land use, and implementation of nutrient management and natural resource conservation practices. Factors internal to estuarine waters that also play a role as drivers are described including biogeochemical processes, physical forces such as wind-driven mixing of the water column, and biological factors such as phytoplankton biomass and the presence of submersed aquatic vegetation. Total nutrient concentrations have been decreasing at most stations in the Potomac River over the long-term, with improvements persisting in the last 10 years as well. These trends follow from the decreasing discharge from TN and TP sources in the watershed. The TP source reductions are not as apparent in the direct loads to the river, which may be part of the reason that tidal nutrient concentrations are not decreasing at as many stations in the short-term as the long-term. While degrading chlorophyll a and Secchi depth trends at several Potomac monitoring stations are concerning, recent improvements in summer oxygen concentrations are promising. The findings that chlorophyll a concentrations in the lower Potomac have either leveled out or improved may suggest a smaller amount of phytoplankton biomass available to fuel summer oxygen depletion. The responses of chlorophyll a, Secchi depth, and bottom DO are mixed in the Potomac tidal waters, but there are multiple possible reasons for this lag in response. Continuing to track water quality response and investigating these possibilities are important steps to understanding water quality patterns and changes in the Potomac River.

Pennsylvania, Virginia, West Viginia↗

James Tributary summary: A summary of trends in tidal water quality and associated factors, 1985-2021

The James Tributary Summary outlines change over time for a suite of monitored tidal water quality parameters and associated potential drivers of those trends for the period 1985 – 2021 and provides a brief description of the current state of knowledge explaining these observed changes. Water quality parameters described include surface (above pycnocline) total nitrogen (TN), surface total phosphorus (TP), surface water temperature (WTEMP), spring and summer (June, July, August) surface chlorophyll a, summer bottom (below pycnocline) dissolved oxygen (DO) concentrations, and Secchi disk depth (a measure of water clarity). Results for annual bottom TP, bottom TN, surface ortho-phosphate (PO4), surface dissolved inorganic nitrogen (DIN), surface total suspended solids (TSS), and summer surface DO concentrations are provided in an Appendix. Drivers discussed include physiographic watershed characteristics, changes in TN, TP, and sediment loads from the watershed to tidal waters, expected effects of changing land use, and implementation of nutrient management and natural resource conservation practices. Factors internal to estuarine waters that also play a role as drivers are described including biogeochemical processes, physical forces such as wind-driven mixing of the water column and increase in rainfall intensity and volume, and biological factors such as phytoplankton biomass and the presence of submerged aquatic vegetation. Continuing to track water quality response and investigating these influencing factors are important steps to understanding water quality patterns and changes in the James River. The intended audiences for this report include, but are not limited to, 1) technical managers within jurisdictions who are looking at tidal water quality data and trying to understand why patterns are occurring, 2) local watershed organizations that are trying to understand these analyses and working to connect them to their local area(s), and 3) federal, state, and academic researchers. Figure 1 presents a conceptual model highlighting these intended audiences. Our goal is for the Tributary Summary documents to be sources of readily available background for change over time in tidal water quality observed with monitoring data. The intended purpose of the Tributary Summary documents is to help answer questions related to water quality, show how landscape factors drive water quality change over time, provide support for management decisions that may alter water quality trends and living resources conditions, and highlight where there may be information or knowledge gaps.

Virginia↗

Puerto Rico’s state of the climate 2014-2021

The climate of Puerto Rico is influenced by the changing global climate. The following chapters present the current knowledge of the geophysical and chemical drivers and signals of global climate change as they affect the climate of Puerto Rico and influence the climate-dependent services, risks, and vulnerabilities that govern human well-being. These include sustainable economic development, delivery of ecosystem services, the conservation of natural and cultural resources, resiliency in built and natural systems, and food security. The chapters draw on global expertise of land, atmosphere, and ocean geophysical interactions associated with increasing greenhouse gases that drive global warming and on local scientific expertise, data, observations, and modeled projections. They present the global warming scenario (section 1), the contribution of Puerto Rico to global climate change as GHG emissions and aerosols (section 2), the context of natural climate variability (section 3), observed and projected trends in temperature (section 4), rainfall (section 5), sea level rise (section 6), ocean acidification and sea surface warming (section 7), and the expected implications of warming climate on tropical cyclones affecting Puerto Rico (section 8).

Puerto Rico↗

Rappahannock tributary summary: A summary of trends in tidal water quality and associated factors, 1985-2022

The Rappahannock Tributary Summary outlines change over time for a suite of monitored tidal water quality parameters and associated potential drivers of those trends for the period of 1985 to 2022, and provides a brief description of the current state of knowledge explaining these observed changes. Water quality parameters described include surface (above pycnocline) total nitrogen (TN), surface total phosphorus (TP), surface water temperature (WTEMP), spring (March-May) and summer (JulySeptember) surface chlorophyll a, summer bottom (below pycnocline) dissolved oxygen (DO) concentrations, and Secchi disk depth (a measure of water clarity). Results for annual bottom TP, bottom TN, surface ortho-phosphate (PO 4 ), surface dissolved inorganic nitrogen (DIN), surface total suspended solids (TSS), and summer surface DO concentrations are provided in Appendix B. Drivers discussed include physiographic watershed characteristics, changes in TN, TP, and sediment loads from the watershed to tidal waters, expected effects of changing land use, and implementation of nutrient management and natural resource conservation practices. Factors internal to estuarine waters that also play a role as drivers are described including biogeochemical processes, physical forces such as winddriven mixing of the water column and increase in rainfall intensity and volume, and biological factors such as phytoplankton biomass and the presence of submerged aquatic vegetation. Continuing to track water quality response and investigating these influencing factors are important steps to understanding water quality patterns and changes in the Rappahannock River. The intended audiences for this report include, but are not limited to, 1) technical managers within jurisdictions who are looking at tidal water quality data and trying to understand why patterns are occurring, 2) local watershed organizations that are trying to understand these analyses and working to connect them to their local area(s), and 3) federal, state, and academic researchers. Figure 1 presents a conceptual model highlighting these intended audiences. Our goal is for the Tributary Summary documents to be sources of readily available background for change over time in tidal water quality observed with monitoring data. The intended purpose of the Tributary Summary documents is to help answer questions related to water quality, show how landscape factors drive water quality change over time, provide support for management decisions that may alter water quality trends and living resources conditions, and highlight where there may be information or knowledge gaps.

Virginia↗

Potomac Tributary Summary: A summary of trends in tidal water quality and associated factors, 1985 - 2022

The Potomac Tributary Summary outlines change over time for a suite of monitored tidal water quality parameters and associated potential drivers of those trends for the period of 1985 to 2022, and provides a brief description of the current state of knowledge explaining these observed changes. Water quality parameters described include surface (above pycnocline) total nitrogen (TN), surface total phosphorus (TP), surface water temperature (WTEMP), spring (March-May) and summer (July-September) surface chlorophyll a, summer bottom (below pycnocline) dissolved oxygen (DO) concentrations, and Secchi disk depth (a measure of water clarity). Results for annual bottom TP, bottom TN, surface ortho-phosphate (PO4), surface dissolved inorganic nitrogen (DIN), surface total suspended solids (TSS), and summer surface DO concentrations are provided in Appendix B. Drivers discussed include physiographic watershed characteristics, changes in TN, TP, and sediment loads from the watershed to tidal waters, expected effects of changing land use, and implementation of nutrient management and natural resource conservation practices. Factors internal to estuarine waters that also play a role as drivers are described including biogeochemical processes, physical forces such as wind driven mixing of the water column and increase in rainfall intensity and volume, and biological factors such as phytoplankton biomass and the presence of submerged aquatic vegetation. Continuing to track water quality response and investigating these influencing factors are important steps to understanding water quality patterns and changes in the Potomac River. The intended audiences for this report include, but are not limited to, 1) technical managers within jurisdictions who are looking at tidal water quality data and trying to understand why patterns are occurring, 2) local watershed organizations that are trying to understand these analyses and working to connect them to their local area(s), and 3) federal, state, and academic researchers. Figure 1 presents a conceptual model highlighting these intended audiences. Our goal is for the Tributary Summary documents to be sources of readily available background for change over time in tidal water quality observed with monitoring data. The intended purpose of the Tributary Summary documents is to help answer questions related to water quality, show how landscape factors drive water quality change over time, provide support for management decisions that may alter water quality trends and living resources conditions, and highlight where there may be information or knowledge gaps.

Maryland, Pennsylvania, Virginia, West Virginia↗

Assessing diet and genotyping success of goat pellet surveys from 2019 in Glacier National Park

Fecal pellets contain genetic information and can be used to identify individuals, their diet, and more. Individual identification can be useful in understanding movements of individuals, developing population estimates, assessing vital rates, genetic diversity and structure, and evaluating trends over time (e.g., Epps et al 2024). Successful genotyping depends on the quality of the sample, which can be influenced by many things including the initial state of the sample, environmental conditions that the pellets were exposed to, and the method of storage. The diet of mountain goats ( Oreamnos americanus ) is hard to study as they are habitat specialists in some of the most remote and rugged alpine environments (Festa-Bianchet & Côté 2012). These iconic ungulates epitomize the renowned wilderness character of Glacier National Park (GNP), yet the population has declined by 45% since 2008 likely due at least in part to changing climate (Graves et al. 2025). This is well above the International Union for the Conservation of Nature’s population decline criterion used to designate a species as vulnerable (IUCN 2012). Mountain goats are known as dietary generalists employing highly variable diets across systems, but diets have not been identified for GNP. Identifying goat diet composition in GNP can provide the information needed to determine whether this population decline could be related to climate-change induced shifts in forage phenology, diversity, productivity, and plant community structure. Other anthropogenic changes in GNP, such as increases in exotic and invasive plant species, will continue to affect plant community composition (Lesica et al. 1993). These alterations may reduce optimal forage availability and quality, which could have a deleterious effect on mountain goat survival. To examine these possibilities, we must first ask- What precisely are the mountain goats of Glacier National Park eating? DNA metabarcoding of fecal pellets provides a cutting-edge, non-invasive method for assessing herbivore diet. Previous studies of mountain goat diet have used methods such as fecal crude protein analysis (Festa-Bianchet & Côté 2012), rumen analysis (Saunders 1955), fecal microhistology (Cobb et al. 2012), and bite-for-bite feeding observations (Daily et al. 1984). Many of these studies have only been able to identify diet components to functional plant type and the methods used are biased towards less digestible diet components such as grasses (McInnis et al. 1983). Metabarcoding of fecal pellets can provide a higher taxonomic resolution than previous methods (Scasta et al. 2019, Stapleton et al. 2022) and open the field for increased participation of volunteers, also called community or citizen scientists. Here, we use mountain goat fecal pellets to conduct 1) an evaluation of which conditions might influence genotyping success and 2) a preliminary assessment of diet using metabarcoding. This pilot study allowed us to evaluate which pellets to focus on collecting, identify the best storage methods, explore approaches for analyzing metabarcoding data, examine the utility of metabarcoding for acquiring taxonomically specific diet information, and to identify genetic sequences that could not be resolved to genus or species levels using these methods. We examined the frequency of occurrence and relative diet composition of identified forbs, shrubs, graminoids, mosses, and trees in mountain goat diets.

Montana↗

Lower Eastern Shore Tributary summary: A summary of trends in tidal water quality and associated factors, 1985-2023

The Lower Eastern Shore Tributary Summary outlines change over time according to a suite of monitored tidal water quality parameters and associated potential drivers of those trends for the period 1985 – 2023, and provides a brief description of the current state of knowledge explaining these observed changes. Water quality parameters described include surface (above pycnocline) total nitrogen (TN), surface total phosphorus (TP), surface water temperature (WTEMP), spring (March-May) and summer (July-September) surface chlorophyll a, summer bottom (below pycnocline) dissolved oxygen (DO) concentrations, and Secchi disk depth (a measure of water clarity). Results for annual bottom TP, bottom TN, surface ortho-phosphate (PO4), surface dissolved inorganic nitrogen (DIN), surface total suspended solids (TSS), and summer surface DO concentrations are provided in an Appendix B. Drivers discussed include physiographic watershed characteristics, changes in TN, TP, and sediment loads from the watershed to tidal waters, expected effects of changing land use, and implementation of nutrient management and natural resource conservation practices. Factors internal to estuarine waters that also play a role as drivers are described including biogeochemical processes, physical forces such as winddriven mixing of the water column and increase in rainfall intensity and volume, and biological factors such as phytoplankton biomass and the presence of submersed aquatic vegetation. Continuing to track water quality response and investigating these influencing factors are important steps to understanding water quality patterns and changes in the Lower Eastern Shore. The intended audiences for this report include, but are not limited to, 1) technical managers within jurisdictions who use tidal water quality to inform management decisions, 2) local watershed organizations that are trying to understand these analyses and working to connect them to their local area(s), and 3) federal, state, and academic researchers. Figure 1 presents a conceptual model highlighting these intended audiences. The Tributary Summary documents are sources of readily available background for change over time in tidal water quality observed with monitoring data. They help answer questions related to water quality, show how landscape factors drive water-quality changes over time, provide support for management decisions that may alter water quality trends and living resources conditions, and highlight where there may be information or knowledge gaps.

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