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At least 415 records · Page 23Linked to original sources

Evaluation of Francisella orientalis ΔpdpA as a live attenuated vaccine against piscine Francisellosis in Nile tilapia

Francisella orientalis is an important bacterial pathogen of marine and freshwater fish with worldwide distribution. Fish francisellosis is a severe subacute to chronic granulomatous disease, with high mortalities and high infectivity rates in cultured and wild fish. To date, there is no approved vaccine for this disease. In this study, we evaluated the efficacy of a defined F. orientalis pathogenicity determinant protein A ( pdpA ) mutant (Δ pdpA ) as a live attenuated immersion vaccine against subsequent immersion challenge with the wild-type organism. Immunized Nile tilapia Oreochromis niloticus were protected (45% relative percent survival) from the lethal challenges and presented significantly lower mortality than nonvaccinated and challenged treatments. Although serum IgM was significantly higher in immunized fish, similar bacterial loads were detected in vaccinated and nonvaccinated survivors. In conclusion, although the F. orientalis Δ pdpA is attenuated and effectively stimulated an adaptive immune response, the low relative percent survival and high bacterial persistence in survivors of immunized and challenged treatments indicates low suitability of Δ pdpA as a mucosal vaccine for tilapia under conditions used in this study.

Journal of Aquatic Animal Health↗

Genomic selection for low salinity tolerance in the eastern oyster Crassostrea virginica in Louisiana and Chesapeake Bay populations

The use of genomic selection (GS) to improve traits of interest in aquaculture species is growing with the availability of genomic resources for those species. The eastern oyster , Crassostrea virginica , is a major aquaculture product and keystone species along the U.S. mid-Atlantic and Gulf coasts. Populations and production of C. virginica are threatened by several environmental stressors, and in certain coastal regions, episodic low salinity conditions have contributed to mass mortality events. Here we examined the potential for genomic selection to improve tolerance to extreme low salinity (≤ 2) in eastern oysters and report the evaluation of GS models for C. virginica from two regions- Louisiana and Chesapeake Bay. Tissue samples from both mortalities and survivors of the lab-based salinity challenges (salinity 2, 28 °C) were genotyped on the 66 K C. virginica SNP array. We performed a genome wide association study (GWAS) for both challenges and then evaluated the effects of several different parameters on GS prediction accuracy, including model type (GBLUP, Bayesian, and weighted), data filtering, Bayesian modelling parameters, and trait encoding (Gaussian, ordinal, and censored). GWAS revealed a significant region on chromosome 1 in the Chesapeake animals while the Louisiana animals showed only a few significantly associated loci. GS model type had no significant impact on prediction accuracy in the Chesapeake animals, but a large impact in the Louisiana animals. Likewise, prediction accuracy remained consistent in the Chesapeake Bay challenge with as few as 5000 markers, while accuracy in the Louisiana challenge began to decline with fewer than 25,000 markers. This likely reflects the lower relatedness among the Louisiana individuals due to unintended wild overset. Overall, with moderate prediction accuracies (∼0.45–0.55) in both regions, GS shows the potential to improve low salinity tolerance in eastern oysters. This will be beneficial to selective breeding programs and restoration efforts in regions where this trait improves oyster survival.

Louisiana, Maryland, Virginia↗

Effects of hexazinone and atrazine on the physiology and endocrinology of smolt development in Atlantic salmon

Exposure to hexazinone (HEX) and atrazine (ATZ), highly mobile and widely used herbicides along rivers in the United States, is potentially harmful to Atlantic salmon, which have been listed as an endangered species. To determine the effects of these contaminants on smolt development, juvenile Atlantic salmon were exposed under flow-through conditions to 100 μg l −1 HEX, 10 and 100 μg l −1 ATZ in fresh water (FW) for 21 days at 10 °C beginning in mid-April. Twelve fish per treatment were sampled in FW, following a 24 h seawater (SW) challenge and after growth for 3 months in SW. Exposure to 100 μg l −1 HEX or 10 μg l −1 ATZ caused no mortalities of smolts in FW or after SW challenge, while 9% of the fish exposed to 100 μg l −1 ATZ died during exposure. Fish exposed to 100 μg l −1 ATZ reduced feeding after 10 days of exposure and had an impaired growth rate in FW and during the first month in SW; compensatory growth occurred in the second and third month in SW. HEX and ATZ at 10 μg l −1 exposure had no effect on plasma levels of cortisol, growth hormone (GH), insulin growth factor I (IGF-I), thyroxine (T 4 ) and plasma 3,5,3′-triiodo- l -thyronine (T 3 ), Cl − , Mg 2+ , Na + , Ca 2+ in FW or after SW challenge. FW smolts exposed to 100 μg l −1 ATZ had decreased plasma Cl − , Mg 2+ , Na + and Ca 2+ ions and increased cortisol. No effect on plasma levels of GH, IGF-I, T 4 or T 3 was found in FW smolts exposed to 100 μg l −1 ATZ. Following SW challenge, fish previously exposed to 100 μg l −1 ATZ had significant increases in hematocrit, plasma cortisol, Cl − , Mg 2+ , Na + , Ca 2+ and a decrease in T 4 and T 3 . It is concluded that under the conditions imposed in this study, HEX does not affect salinity tolerance of Atlantic salmon smolts, while ATZ causes ionoregulatory, growth and endocrine disturbance.

Aquatic Toxicology↗

Markov decision processes in natural resources management: observability and uncertainty

The breadth and complexity of stochastic decision processes in natural resources presents a challenge to analysts who need to understand and use these approaches. The objective of this paper is to describe a class of decision processes that are germane to natural resources conservation and management, namely Markov decision processes, and to discuss applications and computing algorithms under different conditions of observability and uncertainty. A number of important similarities are developed in the framing and evaluation of different decision processes, which can be useful in their applications in natural resources management. The challenges attendant to partial observability are highlighted, and possible approaches for dealing with it are discussed.

Ecological Modelling↗

Early viral replication and induced or constitutive immunity in rainbow trout families with differential resistance to Infectious hematopoietic necrosis virus (IHNV)

The main objective of this study was to assess correlates of innate resistance in rainbow trout full-sibling families that differ in susceptibility to Infectious hematopoietic necrosis virus (IHNV). As part of a commercial breeding program, full-sibling families were challenged with IHNV by waterborne exposure at the 1 g size to determine susceptibility to IHNV. Progeny from select families (N = 7 families) that varied in susceptibility (ranging from 32 to 90% cumulative percent mortality (CPM)) were challenged again at the 10 g size by intra-peritoneal injection and overall mortality, early viral replication and immune responses were evaluated. Mortality challenges included 20–40 fish per family while viral replication and immune response studies included 6 fish per family at each time point (24, 48 and 72 h post-infection (hpi)). CPM at the 1 g size was significantly correlated with CPM at the 10 g size, indicating that inherent resistance was a stable trait irrespective of size. In the larger fish, viral load was measured by quantitative reverse-transcriptase PCR in the anterior kidney and was a significant predictor of family disease outcome at 48 hpi. Type I interferon (IFN) transcript levels were significantly correlated with an individual's viral load at 48 and 72 hpi, while type II IFN gene expression was significantly correlated with an individual's viral load at 24 and 48 hpi. Mean family type I but not type II IFN gene expression was weakly associated with susceptibility at 72 hpi. There was no association between mean family susceptibility and the constitutive expression of a range of innate immune genes (e.g. type I and II IFN pathway genes, cytokine and viral recognition receptor genes). The majority of survivors from the challenge had detectable serum neutralizing antibody titers but no trend was observed among families. This result suggests that even the most resistant families experienced sufficient levels of viral replication to trigger specific immunity. In summary, disease outcome for each family was determined very early in the infection process and resistance was associated with lower early viral replication.

Fish and Shellfish Immunology↗

Stakeholder attitudes and perspectives on wildlife disease surveillance as a component of a One Health approach in Thailand

Coordinated wildlife disease surveillance (WDS) can help professionals across disciplines effectively safeguard human, animal, and environmental health. The aims of this study were to understand how WDS in Thailand is utilized, valued, and can be improved within a One Health framework. An online questionnaire was distributed to 183 professionals (55.7% response rate) across Thailand working in wildlife, marine animal, livestock, domestic animal, zoo animal, environmental, and public health sectors. Twelve semi-structured interviews with key professionals were then performed. Three-quarters of survey respondents reported using WDS data and information. Sectors agreed upon ranking disease control (76.5% of respondents) as the most beneficial outcome of WDS, while fostering new ideas through collaboration was valued by few participants (2.0%). Accessing data collected by ones own sector was identified as the most challenging (50%) yet least difficult to improve (88.3%). Having legal authority to conduct WDS was the second most frequently identified challenge. Interviewees explained that legal documentation required for crossinstitutional collaborations posed a barrier to efficient communication and use of human resources. Survey respondents identified allocation of human resources (75.5%), adequate budget (71.6%), and having a clear communication system between sectors (71.6%) as highest priority areas for improvement to WDS in Thailand. Authorization from administrative officials and support from local community members were identified as challenges during in-person interviews. Future outreach should be directed towards these groups. As 42.9% of marine health professionals had difficulty knowing whom to contact in other sectors and 28.4% of survey respondents indicated that communication with marine health professionals was not applicable to their work, connecting the marine sector with other sectors may be prioritized. This study identifies priorities for addressing current challenges in the establishment of a general WDS system and information management system in Thailand while presenting a model for such evaluation in other regions.

One Health Newsletter↗

Recombinant raccoon pox vaccine protects mice against lethal plague

Using a raccoon poxvirus (RCN) expression system, we have developed new recombinant vaccines that can protect mice against lethal plague infection. We tested the effects of a translation enhancer (EMCV-IRES) in combination with a secretory (tPA) signal or secretory (tPA) and membrane anchoring (CHV-gG) signals on in vitro antigen expression of F1 antigen in tissue culture and the induction of antibody responses and protection against Yersinia pestis challenge in mice. The RCN vector successfully expressed the F1 protein of Y. pestis in vitro. In addition, the level of expression was increased by the insertion of the EMCV-IRES and combinations of this and the secretory signal or secretory and anchoring signals. These recombinant viruses generated protective immune responses that resulted in survival of 80% of vaccinated mice upon challenge with Y. pestis . Of the RCN-based vaccines we tested, the RCN-IRES-tPA-YpF1 recombinant construct was the most efficacious. Mice vaccinated with this construct withstood challenge with as many as 1.5 million colony forming units of Y. pestis (7.7×10 4 LD 50 ). Interestingly, vaccination with F1 fused to the anchoring signal (RCN-IRES-tPA-YpF1-gG) elicited significant anti-F1 antibody titers, but failed to protect mice from plague challenge. Our studies demonstrate, in vitro and in vivo, the potential importance of the EMCV-IRES and secretory signals in vaccine design. These molecular tools provide a new approach for improving the efficacy of vaccines. In addition, these novel recombinant vaccines could have human, veterinary, and wildlife applications in the prevention of plague.

Vaccine↗

A synergistic future for AI and ecology

Research in both ecology and AI strives for predictive understanding of complex systems, where nonlinearities arise from multidimensional interactions and feedbacks across multiple scales. After a century of independent, asynchronous advances in computational and ecological research, we foresee a critical need for intentional synergy to meet current societal challenges against the backdrop of global change. These challenges include understanding the unpredictability of systems-level phenomena and resilience dynamics on a rapidly changing planet. Here, we spotlight both the promise and the urgency of a convergence research paradigm between ecology and AI. Ecological systems are a challenge to fully and holistically model, even using the most prominent AI technique today: deep neural networks. Moreover, ecological systems have emergent and resilient behaviors that may inspire new, robust AI architectures and methodologies. We share examples of how challenges in ecological systems modeling would benefit from advances in AI techniques that are themselves inspired by the systems they seek to model. Both fields have inspired each other, albeit indirectly, in an evolution toward this convergence. We emphasize the need for more purposeful synergy to accelerate the understanding of ecological resilience whilst building the resilience currently lacking in modern AI systems, which have been shown to fail at times because of poor generalization in different contexts. Persistent epistemic barriers would benefit from attention in both disciplines. The implications of a successful convergence go beyond advancing ecological disciplines or achieving an artificial general intelligence—they are critical for both persisting and thriving in an uncertain future.

Proceedings of the National Academy of Sciences↗

A transdisciplinary approach to growing an applied science of cultural evolution for a sustainable future

Addressing sustainability challenges requires an integrative approach that bridges scientific research with practical application. The field of cultural evolution (CE) offers a perspective that may guide transitions and cultural transformations for a sustainable future. However, there have been few efforts to apply this field to sustainability challenges. This study explores how CE can inform sustainability practices through mechanisms of social learning, processes of cultural adaptation and conditions that promote cooperative governance. Through a two-phase research design—comprising online discussions with professionals from six domains, and an international workshop—we examined the perceived benefits, challenges and opportunities for applying CE in real-world contexts. Our findings indicate that professionals recognize CE’s potential to enhance knowledge dissemination, foster adaptation to social–ecological changes, improve governance structures and generate cooperation. However, barriers such as complex terminology, unfamiliarity with CE, the lack of clear, context-specific evidence of added value and competition with existing frameworks hinder its application. To overcome these challenges, we propose simplifying CE concepts, demonstrating its unique contributions, and continuing to foster co-production with practitioners to refine its applicability. This study is an initial step in building an applied science of CE that can support sustainability transformations across diverse domains.

Philosophical Transactions of the Royal Society B ↗

Accuracy, accessibility, and institutional capacity shape the utility of habitat models for managing and conserving rare plants on western public lands

Public lands are often managed for multiple uses ranging from energy development to rare plant conservation. Habitat models can help land managers assess and mitigate potential effects of projects on rare plants, but it is unclear how models are currently being used. Our goal was to better understand how staff in the Bureau of Land Management currently use habitat models to inform their decisions, and perceived challenges and benefits associated with that use. We first examined litigation documents to determine whether the agency has been challenged on its use of data for rare plants and found no relevant legal challenges. Second, we analyzed model use in National Environmental Policy Act (NEPA) documents and found no clear citations of habitat models. Finally, we conducted interviews with agency staff who analyze potential effects of proposed actions on rare plants in NEPA documents. The primary challenges interviewees faced in using models related to data organization and access, model quality and accuracy, and institutional capacity. Interviewees believed models could be used more to inform decisions and actions to conserve rare plants and rare plant habitat on public lands and recommended improving staff access to models, creating models for additional species, and addressing staffing limitations.

Arizona, California, Colorado, New Mexico, Utah, W↗

In-Flight performance of MESSENGER's Mercury dual imaging system

The Mercury Surface, Space ENvironment, GEochemistry, and Ranging (MESSENGER) spacecraft, launched in August 2004 and planned for insertion into orbit around Mercury in 2011, has already completed two flybys of the innermost planet. The Mercury Dual Imaging System (MDIS) acquired nearly 2500 images from the first two flybys and viewed portions of Mercury's surface not viewed by Mariner 10 in 1974-1975. Mercury's proximity to the Sun and its slow rotation present challenges to the thermal design for a camera on an orbital mission around Mercury. In addition, strict limitations on spacecraft pointing and the highly elliptical orbit create challenges in attaining coverage at desired geometries and relatively uniform spatial resolution. The instrument designed to meet these challenges consists of dual imagers, a monochrome narrow-angle camera (NAC) with a 1.5?? field of view (FOV) and a multispectral wide-angle camera (WAC) with a 10.5?? FOV, co-aligned on a pivoting platform. The focal-plane electronics of each camera are identical and use a 1024??1024 charge-coupled device detector. The cameras are passively cooled but use diode heat pipes and phase-change-material thermal reservoirs to maintain the thermal configuration during the hot portions of the orbit. Here we present an overview of the instrument design and how the design meets its technical challenges. We also review results from the first two flybys, discuss the quality of MDIS data from the initial periods of data acquisition and how that compares with requirements, and summarize how in-flight tests are being used to improve the quality of the instrument calibration. ?? 2009 SPIE.

Conference Paper↗

Improving how science informs policy within the Ecosystem Approach

Science is fundamental to sound policies, particularly when it comes to implementing an Ecosystem Approach. Science can and should inform nearly all facets of an Ecosystem Approach, yet challenges remain to realizing this goal. To help identify and better understand these challenges we used a qualitative comparative case study approach to identify and characterize the challenges and successes of implementing a science-driven Ecosystem Approach in the Laurentian Great Lakes. These case studies include delisting of Areas of Concern, improving coastal resilience, and addressing declining offshore lake productivity. These case studies were selected because they provide a set of very different, yet complementary, cases for assessing implementation, as well as the factors influencing the science-policy exchange. Through this comparative study, we identified a diverse set of challenges and successes, that were both systemic and case specific. Emerging from this comparative assessment were principles and enabling conditions (e.g. scale, governance, shared goals) we believe are critical to consider when establishing or improving a science-driven Ecosystem Approach.

Aquatic Ecosystem Health & Management↗

Furunculosis in brook trout: Infection by contact exposure

In juvenile brook trout (Salvelinus fontinalis) challenged by applying about 10 9 virulent Aeromonas salmonicida cells to an abraded area along the lateral line, or held in aquarium water containing about 10 5 cells per milliliter, mortalities were 50 and 60%, respectively, within 7 days after exposure. Neither feeding the bacterium to the fish nor applying it directly to the gills caused systemic bacterial infection. Two consistent experimental challenges were developed for inducing furunculosis in juvenile brook trout: (1) 15‐min contact (bath) exposures of trout to about 10 6 A. salmonicida cells per milliliter of spring water, and (2) 60‐s dip exposures of the fish to 10 9 A. salmonicida cells per milliliter. These challenge procedures produced 70‐100% and 74‐88% mortality, respectively, within 14 days after exposure. Results obtained with a strain of brook trout from Owhi Lake, Washington, by the dip exposure method were affected by neither the age of the fish (up to 1 year) nor the source of the population. The challenge was also effective for Atlantic salmon (Salmo salar) but not for rainbow trout (S. gairdneri).

Progressive Fish-Culturist↗

Immersion and injection vaccination of salmonids against furunculosis with an avirulent strain of Aeromonas salmonicida

Replicate groups of brook trout (Salvelinus fontinalis) immunized by intraperitoneal injection of graded dilutions of an avirulent strain of Aeromonas salmonicida were protected from a virulent challenge. Mean mortality after challenge was 88% in control trout, 66% in trout immunized with 3 × 10 6 or 3 × 10 5 cells, and 39% in trout immunized with 3 × 10 4 to 3 × 10¹ cells. In brook trout challenged after immersion for 60 s in a culture containing 2.3 × 10 9 avirulent cells of A. salmonicida per milliliter, mortality was 44%, compared with 94% in controls. In Atlantic salmon (Salmo salar), similarly vaccinated by immersion, mortality was 12.5% after challenge compared with 87.5% in controls. In additional studies, mean mortality of Atlantic salmon vaccinated by immersion was 14%, whereas that of control salmon was 92%.

Progressive Fish-Culturist↗

Reversion to virulence and efficacy of an attenuated canarypox vaccine in Hawai'i 'Amakihi ( Hemignathus Virens )

Vaccines may be effective tools for protecting small populations of highly susceptible endangered, captive-reared, or translocated Hawaiian honeycreepers from introduced Avipoxvirus , but their efficacy has not been evaluated. An attenuated Canarypox vaccine that is genetically similar to one of two passerine Avipoxvirus isolates from Hawai‘i and distinct from Fowlpox was tested to evaluate whether Hawai‘i ‘Amakihi ( Hemignathus virens ) can be protected from wild isolates of Avipoxvirus from the Hawaiian Islands. Thirty-one (31) Hawai‘i ‘Amakihi were collected from high-elevation habitats on Mauna Kea Volcano, where pox transmission is rare, and randomly divided into two groups. One group was vaccinated with Poximune C®, whereas the other group received a sham vaccination with sterile water. Four of 15 (27%) vaccinated birds developed life-threatening disseminated lesions or lesions of unusually long duration, whereas one bird never developed a vaccine-associated lesion or “take.” After vaccine lesions healed, vaccinated birds were randomly divided into three groups of five and challenged with either a wild isolate of Fowlpox (FP) from Hawai‘i, a Hawai‘i ‘Amakihi isolate of a Canarypox-like virus (PV1), or a Hawai‘i ‘Amakihi isolate of a related, but distinct, passerine Avipoxvirus (PV2). Similarly, three random groups of five unvaccinated ‘Amakihi were challenged with the same virus isolates. Vaccinated and unvaccinated ‘Amakihi challenged with FP had transient infections with no clinical signs of infection. Mortality in vaccinated ‘Amakihi challenged with PV1 and PV2 ranged from 0% (0/5) for PV1 to 60% (3/5) for PV2. Mortality in unvaccinated ‘Amakihi ranged from 40% (2/5) for PV1 to 100% (5/5) for PV2. Although the vaccine provided some protection against PV1, both potential for vaccine reversion and low efficacy against PV2 preclude its use in captive or wild honeycreepers.

Hawaii↗

The ecology, restoration, and management of southeastern floodplain ecosystems: A synthesis

Floodplain ecosystems of the southeastern United States provide numerous services to society, but hydrologic and geomorphic alterations, agricultural practices, water quality and availability, and urban development continue to challenge restorationists and managers at multiple spatial and temporal scales. These challenges are further exacerbated by tremendous uncertainty regarding climate and land use patterns and natural variability in these systems. The symposium from which the papers in 2009 ensued was organized to provide a critical evaluation of current natural resource restoration and management practices to support the sustainability of floodplain ecosystem functions in the southeastern United States. In this paper we synthesize these concepts and evaluate restoration and conservation techniques in light of our understanding of these ecosystems. We also discuss current and future challenges and attempt to identify new approaches that may facilitate the long-term sustainability of southeastern floodplain systems. We conclude that integration of disciplines and approaches is necessary to meet the floodplain conservation challenges of the coming century. Integration will not only include purposeful dialogue between interdisciplinary natural resource professionals, but it also is necessary to sincerely engage the public about goals, objectives, and desirable outcomes of floodplain ecosystem restoration.

Southeastern Floodplain systems↗

Colorado River Basin Actionable and Strategic Integrated Science and Technology Project—Science strategy

The U.S. Geological Survey (USGS) conducts a wide variety of science that improves understanding of droughts and their effects on ecosystems and society. This work includes data collection and monitoring of aquatic and terrestrial systems; assessment and analysis of patterns, trends, drivers, and impacts of drought; development and application of predictive models; and delivery of information and decision-making tools to stakeholders. Stakeholders, which include Federal, Tribal, State, and local agencies, nongovernmental organizations, and others, use this information to anticipate, assess, react to, and mitigate drought conditions and impacts. There is no obvious near-term solution to reduce the frequency and severity of droughts or to mitigate drought impacts. Multidecadal drought is a “grand challenge” that benefits from integration of existing technolo­gies, data, knowledge, and models across related and dispa­rate disciplines, facilitated by new science and technology. In response, the USGS initiated a new integrated-science approach in the Colorado River Basin in 2020. The Colorado River Basin was specifically selected because of concerns about future drought and its consequences for the region. This document explains how the Colorado River Basin Actionable and Strategic Integrated Science and Technology (ASIST) project extends and enhances the science supported by USGS Mission Areas and Programs and articulates scientific gaps and stakeholder needs to identify and reduce drought risks. The approach seeks to answer complex Earth science questions developed in partner­ship with stakeholders about severe long-term drought. An inte­grated approach is required to tackle these complex questions, which any single science discipline cannot answer on its own. In addition to increased understanding of drought and drought effects in complex systems, the Colorado River Basin ASIST project was designed to improve efficiencies through rapid location of a broad array of data sources, assembly of model-ready multidisciplinary data, and delivery of actionable science to stakeholders at the speeds and scales needed for deci­sion making. The project team identified the following actions needed for USGS to implement and advance an integrated science approach in the Colorado River Basin: (1) engage with stakeholders to document their needs and iteratively co-produce science and science delivery tools to address these needs, (2) integrate monitoring and observation systems developed by USGS and other agencies that track droughts and their effects, (3) collect and provide analysis-ready data to support integrated applications, (4) integrate data and model connections to predict multiple drought impacts, (5) conduct multidisciplinary coor­dination to improve interpretations, (6) leverage the knowledge base across USGS to enhance decision making, and (7) support the development of new integrated science approaches and technologies that provide analysis and management tools that can be used to adapt to the effects of drought in the Colorado River Basin. Proposals for initial short-term use-case projects were solicited, a subset of which was selected for funding to test implementation of these actions. Additionally, the project organized and convened a series of science and technology collaboration workshops in the USGS focused on challenges that were identified and prioritized by the short-term use-case projects and during the initial stakeholder analysis. These work­shops were designed to bring together diverse perspectives to discuss science and technology challenges, stakeholder needs, capabilities, and knowledge gaps, with the goal of determin­ing how the USGS can address challenges, identify future opportunities for continued engagement between participants, and inform the next steps for the Colorado River Basin ASIST project. Continuing to collaboratively engage with a wide range of stakeholders using an integrated approach will provide a suitable foundation of data and tools to formulate actionable intelligence for predicting droughts and informing adaptation to the effects of long-term drought in a holistic, timely, and effective manner.

Arizona, California, Colorado, Nevada, New Mexico,↗

U.S. Geological Survey (USGS), Western Region: Coastal ecosystem responses to influences from land and sea, Coastal and Ocean Science

Sea otters and the nearshore ecosystems they inhabit-from highly urbanized California to relatively pristine Alaska-are the focus of a new multidisciplinary study by scientists with the U.S. Geological Survey (USGS) and a suite of international, academic and government collaborators. The Coastal Ecosystem Responses to Influences from Land and Sea project will investigate the many interacting variables that influence the health of coastal ecosystems along the Northeast Pacific shore. These ecosystems face unprecedented challenges, with threats arising from the adjacent oceans and lands. From the ocean, challenges include acidification, sea level rise, and warming. From the land, challenges include elevated biological, geological and chemical pollutants associated with burgeoning human populations along coastlines. The implications of these challenges for biological systems are only beginning to be explored. Comparing sea otter population status indicators from around the northeastern Pacific Rim, will begin the process of defining factors of coastal ecosystem health in this broad region.

Fact Sheet↗