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Reference samples for use in analytical geochemistry: their availability, preparation, and appropriate use

A large number of certified and other reference samples are available for use in analytical geochemistry. Certified materials are preferred, but of much more limited availability than other reference samples for most geochemical applications. The availability of rock, sediment, soil, water, and plant reference samples is outlined; ore and mineral separate reference samples are not included in the discussion. The preparation of these materials, including the establishment of certified or recommended concentrations, is then reviewed. It is shown that comparable quality can be achieved for both certified and recommended concentrations, though it has not always been achieved in the past. Finally, the most appropriate ways to use reference samples in quality control and instrumental calibration are discussed.

Journal of Geochemical Exploration↗

Contemporary human impacts on alpine ecosystems: the direct and indirect effects of human-induced climate change and land use

Alpine ecosystems account for ca. 3 % of terrestrial habitats yet, along with adjacent mountain systems, provide water resources to nearly half of the world’s human population. Approximately 20 % of humans live in or near mountain areas, making it inherently important to understand current impacts on these systems. Here, I review literature regarding current and projected human impacts on alpine ecosystems, including the direct and indirect impacts of human-induced climate change on alpine plant, animal, and soil communities. I also discuss the influence of recreation and tourism, grazing, and other land use changes including the introduction of non-native and invasive species in alpine systems. I conclude with management implications as well as future areas of research needed to better understand changes to these systems.

Book chapter↗

Season of grazing interacts with soil texture, selecting for associations of biocrust morphogroups

Livestock grazing, a widespread land use in semi-arid systems, is often placed in opposition to the perpetuation of biological soil crusts (“biocrusts”: lichens, mosses, and algal crusts including cyanobacteria) that live on the soil surface and provide ecosystem functions. The composition of biocrusts and vascular plants varies with climate, soils, and disturbance. In general, ruderal mosses and light algal crusts make up greater proportions of biocrusts in the presence of disturbance, although morphogroups of biocrusts respond differently to various disturbances. It is unknown if there are scenarios under which grazing can occur and ruderal components of biocrust could be maintained. We examine the hypothesis that soil surface texture-moisture interactions influence the ability of biocrusts to withstand trampling, reasoning that finer-textured soils are firmer (therefore serving as a better substrate for biocrusts) when dry and that coarser-textured are firmer when wet. We test these relationships within Birds of Prey, National Conservation Area (Boise, Idaho, USA). Results demonstrate two associations of biocrusts, dependent on season of grazing: one dominated by light algal crusts and lichens that frequently occurs with wet season grazing, and a second dominated by tall mosses and cup lichens that frequently occurs with dry season grazing. High cover of the invasive annual grass, Bromus tectorum (L.) was observed on sites with coarse-textured soils, and high sand content, that are grazed at relatively high intensities, creating unstable surfaces, and likely putting biocrusts at greater susceptibility to trampling. Results suggest that livestock management that accounts for soil texture and moisture could be used to maintain cover of ruderal biocrusts on fine-textured soils, that are grazed in the dry season, at low intensity. We discuss our findings in the context of managing for species of interest. Our findings are timely as varying the season of grazing is increasingly discussed as a means of favoring desirable native perennial grasses. Although ruderal morphogroups of biocrusts are not interpreted as having equivalent ecosystem functions compared to intact biocrusts, their contributions to soil stability, fertility, hydrology, and weed abatement could increase if they were more intentionally targeted by management.

Geoderma↗

A recording evaporimeter

The instrument herein described was originally designed and built to record the evaporation‐loss from a standard Weather Bureau pan for use in a study of the variation of flow in Santa Ana River. Valuable suggestions were made by various members of the Water Resources Branch of the Geological Survey in Southern California, and financial assistance for construction was given by F. C. Ebert and H. C. Troxell of the same organization. The typing of the paper and some of the drafting were done by Works Progress Administration help. The original instrument was damaged by flood‐waters in 1934, while in operation at Baldwin Park, California. It was then redesigned and constructed of stainless materials and installed on the campus of the San Bernardino Valley Junior College. The Weather Bureau pan was replaced by a thermally insulated pan. The damping unit was added at this time by the present writers. The evaporimeter was placed in regular operation on October 30, 1937, in a study of the relative magnitudes of the various energy‐components associated with solar and sky radiation and with evaporation from water‐surfaces, from damp soil, and from plants.

Eos, Transactions, American Geophysical Union↗

Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy

FTIR and Raman spectromicroscopy were used to characterize the composition of Curvularia protuberata hyphae, and to compare a strain isolated from plants inhabiting geothermal soils with a non-geothermal isolate. Thermal IR source images of hyphae have been acquired with a 64 × 64 element focal plane array detector; single point IR spectra have been obtained with synchrotron source light. In some C . protuberata hyphae, we have discovered the spectral signature of crystalline mannitol, a fungal polyol with complex protective roles. With FTIR-FPA imaging, we have determined that the protein content in cells remains fairly constant throughout the length of a hypha, whereas the mannitol is found at discrete, irregular locations. This is the first direct observation of mannitol in intact fungal hyphae. Since the concentration of mannitol in cells varies with respect to position and is not present in all hyphae, this discovery may be related to habitat adaptation, fungal structure and growth stages.

Analyst↗

Optimizing available network resources to address questions in environmental biogeochemistry

An increasing number of network observatories have been established globally to collect long-term biogeochemical data at multiple spatial and temporal scales. Although many outstanding questions in biogeochemistry would benefit from network science, the ability of the earth- and environmental-sciences community to conduct synthesis studies within and across networks is limited and seldom done satisfactorily. We identify the ideal characteristics of networks, common problems with using data, and key improvements to strengthen intra- and internetwork compatibility. We suggest that targeted improvements to existing networks should include promoting standardization in data collection, developing incentives to promote rapid data release to the public, and increasing the ability of investigators to conduct their own studies across sites. Internetwork efforts should include identifying a standard measurement suite—we propose profiles of plant canopy and soil properties—and an online, searchable data portal that connects network, investigator-led, and citizen-science projects.

BioScience↗

Scaling from microsite to landscape to resolve litter decomposition dynamics in globally extensive drylands

1. Decomposition controls the release of carbon and nutrients from decaying plant litter into soils or the atmosphere. In most biomes decomposition rates can be accurately predicted with simple mathematical models, but these models have long under-predicted decomposition in globally- extensive drylands. 2. We posit that the exposed surface conditions characteristic of drylands makes litter decomposition uniquely subject to microsite-specific environmental controls and spatially-variable microbial communities. As such, decomposition in dryland ecosystems – which are characterized by extremes in temporal heterogeneity of climate conditions and spatial heterogeneity of vegetation cover with corresponding microclimate variability – is a prime example of a macrosystems process that can be addressed by merging field data with new predictive models operating across a hierarchical continuum of spatial scales and process resolutions. 3. A macrosystems approach offers promise to reconcile model-measurement discrepancies by integrating observations and experiments across multiple scales, from microsites (e.g., shrub sub-canopy or intercanopy) to regions (e.g., across a 100s of km2 study site with complex topography, precipitation, and temperature) and ultimately to a continental perspective (e.g., North American drylands). 4. Recent developments in technology and data availability position the scientific community to integrate lab, field, modeling, and remote sensing approaches across a hierarchical range of scales to capture the spatiotemporal distribution of litter and environmental conditions needed to predict decay dynamics at the micro-to-macroscale. This multi-scale approach promises a path forward to resolving a longstanding disconnect between measured and modeled data in dryland litter decomposition. 5. Dryland litter decomposition presents an excellent case study for resolving spatially and temporally complex biogeochemical dynamics through a hierarchical, multidisciplinary macrosystems approach. 6. We focus on dryland litter decomposition, but the hierarchical, multidisciplinary macrosystems approach we outline shows great potential for resolving other spatially and temporally complex biogeochemical processes across a wide range of ecosystems.

Functional Ecology↗

Secondary invasion following the reduction of Coronilla varia (Crownvetch) in sand prairie

I investigated the effect of Coronilla varia invasion and subsequent reduction on the plant community and soil nitrogen availability in a degraded Illinois sand prairie. The presence of C. varia significantly increased soil nitrogen availability and significantly decreased native species richness and cover, but neither helped nor hindered a common non-native grass, Poa pratensis . One year after the drastic reduction of C. varia , soil nitrogen remained somewhat elevated and native species richness and cover low, but P. pratensis cover increased nearly six-fold. These results suggest that C. varia 's impact might be continued through altered soil nitrogen. More important, however, was the strong response of P. pratensis , which could hinder restoration efforts by competing with native species.

American Midland Naturalist↗

Elk response to the La Mesa fire and current status in the Jemez Mountains

Faunal remains in local archeological sites and historic information suggest that elk populations in the Jemez Mountains were low from ca. 1200 A.D. through ca. 1900 A.D., when they were extirpated from this region. Elk were reintroduced to the Jemez country in 1948 and 1964- 1965, and their population apparently grew exponentially, reaching 1000 animals in the 1970's and about 7000 by 1991. Elk populations in Bandelier National Monument and adjoining areas increased rapidly after the 1977 La Mesa Fire. Winter use by elk in the La Mesa Fire area, centered on Bandelier, grew from about 100 animals in .1978 to around 1500 elk by 1992. The dramatic increase in the Bandelier elk herd (an annual growth rate of 21.3% and a 3.6 year population doubling time) was due in part to the creation of about 6000 hectares of grassy winter range in and around the park by the La Mesa Fire. Some of this local population increase reflects concentration of elk into this favorable wintering habitat from surrounding portions of the Jemez Mountains. Existing data are inadequate to determine whether elk populations are still growing rapidly in the Jemez Mountains. While annual aerial surveys since 1990 in Bandelier reveal no clear population trend, a variety of observations demonstrate increasing elk use of lower elevation areas. Negative resource impacts from today's high elk populations are beginning to be widely noted across the Jemez Mountains, especially in high-use portions of the Bandelier National Monument area. Affected resources range from plant communities to soils and even archeological sites. Given the large uncertainties associated with the current data on elk populations, care should be taken to avoid further population increases until the resource impacts of this new phenomenon (large numbers of elk) can be identified, desirable population levels identified (based to a significant degree upon ecological information and resource carrying capacities, as well as social considerations), and appropriate cooperative management strategies implemented.

General Technical Report↗

Identification of potential wetlands in training areas on Ravenna Army Ammunition Plant, Ohio, and guidelines for their management

Potential wetlands in training areas on Ravenna Army Ammunition Plant, Ohio, were mapped by use of geographic information system (GIS) data layers and field inspection. The GIS data layers were compiled from existing sources and interpretation of aerial photography. Data layers used in the GIS analysis were wetland-plant communities, hydric soils, National Wetlands Inventory designated areas, and wet areas based on photogrammetry. According to review of these data layers, potential wetlands constitute almost one-third of the land in the training areas. A composite map of these four data layers was compiled for use during inspection of the training areas. Field inspection focused on the presence of hydrophytic vegetation and macroscopic evidences of wetland hydrology. Results of the field inspection were in general agreement with those predicted by the GIS analysis, except that some wet areas were more extensive than predicted because of high amounts of precipitation during critical periods of 1995 and 1996. Guidelines for managing wetlands in the training areas are presented.

Open-File Report↗

Lessons learned from wetlands research at the Cottonwood Lake Study Area, Stutsman County, North Dakota, 1967–2021

Depressional wetlands in the Prairie Pothole Region of North America have a long history of investigation owing to their importance in maintaining migratory-bird populations, especially waterfowl. One area of particularly intensive study is the Cottonwood Lake study area in Stutsman County, North Dakota. Studies at the Cottonwood Lake study area began in 1967 and continue through the present (2022). During this period of scientific discovery, meteorological conditions at the Cottonwood Lake study area varied greatly and included one of the most severe droughts of the 20th century and one of the wettest periods in the past 500 years. Persistent wet conditions that began in 1993 have contributed to state changes in many of the study area’s larger wetlands to lake-like conditions, whereas the smaller wetlands returned to seasonally ponded conditions during relatively dry years interspersed within the longer-term wet period. Additionally, some nonwetland areas of the study area developed wetland plant, hydrology, and soil characteristics during the 1993-to-present (2022) wet period. The persistently high stages of water in the larger wetlands since 1993 contributed to a buildup of dissolved solids and increases in salinity with time following an initial decrease in salinity caused by the dilution of dissolved solids within a larger volume of water. During 2021, drought conditions similar to the 1988 to 1992 period may develop if conditions persist. However, meteorological changes during the past 30 years have persisted long enough to be considered a change in climate conditions at the study area and, if such wet conditions continue, would represent a change from conditions that occurred in the past two millennia. During the period of study covered in this report (1967–2021), biotic communities responded in a variety of ways to subtle and marked changes in ponded-water depths, permanence, and salinity among the different wetland types in the study area. This report provides background information on the Cottonwood Lake study area and its context within the Prairie Pothole Region, documents techniques used to quantify environmental conditions and biotic communities, describes major trends that have been observed, presents significant findings as “lessons learned,” discusses recent modeling advances, and highlights key messages to managers. The Wetland Continuum concept was used as a framework to place research findings within an ecological context and to highlight the dynamic nature of prairie-pothole wetland ecosystems.

North Dakota↗

Evidence for nutrient enrichment of high-elevation lakes in the Sierra Nevada, California

Long-term measurements (1983-2001) of nutrients and seston in Emerald Lake (Sierra Nevada, California) have revealed ecologically significant patterns. Nitrate, both during spring runoff and during growing seasons, declined from 1983 through 1995. Declining snowmelt nitrate was caused primarily by changes in snow regime induced by the 1987-1992 drought: years with shallow, early melting snowpacks had lower snowmelt nitrate concentrations owing to less labile N production in catchment soils and longer plant growing seasons. However, nitrate declines during growing seasons carried through the wetter years of 1993-2000 and are likely the result of increased P loading to the lake and the release of phytoplankton from P limitation. Contemporaneous with these changes was an increase in algal biomass and a shift from P limitation toward more frequent N limitation of phytoplankton abundance. Particulate carbon concentrations in the late 1990s were two- to threefold greater than in the early 1980s. These trends were reflected in a larger set of Sierra Nevada lakes sampled as part of synoptic surveys (n = 28). Between 1985 and 1999, nitrate decreased and total P increased in >70% of the lakes sampled. Our data suggest that lakes throughout the Sierra Nevada are experiencing measurable eutrophication in response to the atmospheric deposition of nutrients.

Limnology and Oceanography↗

Dicofol (Kelthane) as an environmental contaminant: A review

Dicofol is persistent in soil and on plants. No compelling evidence exists that dicofol breaks down or is metabolized to DDTr in nature. Dicofol does not accumulate in birds as rapidly as DDE, and it has reproductive effects that are less harmful than DDE. Fish, birds, and mammals are reproductively sensitive to dicofol products, but levels presently found in wildlife are below levels shown experimentally to cause significant harm. Eggs of fish-eating wild birds from citrus, cotton, and apple-growing areas should be analyzed for dicofol residues. Nest success of fish-eating birds in the most contaminated populations should be studied to evaluate the environmental effects of dicofol.

Fish and Wildlife Technical Report↗

A complex-systems approach to predicting effects of sea level rise and nitrogen loading on nitrogen cycling in coastal wetland ecosystems

To effectively manage coastal ecosystems, we need an improvedunderstanding of how tidal marsh ecosystem services will respond to sea-level rise and increased nitrogen (N) loading to coastal areas. Here we review existing literature to better understand how these interacting perturbations s will likely impact N removal by tidal marshes. We propose that the keyy factors controlling long-term changes in N removal are plant-community changes, soil accretion rates, surface-subsurface flow paths, marsh geomorphology microbial communities, and substrates for microbial reactions. Feedbacks affecting relative elevations and sediment accretion ratess will serve as dominant controls on future N removal throughout the marsh. Given marsh persistence, we hypothesize that the processes dominating N removal will vary laterally across the marsh and longitudinallyalong the estuarine gradient. In salt marsh interiors, where nitrate reduction rates are often limited by delivery of nitrate to bacterial communities, reductions in groundwater discharge due to sea level rise may trigger a net reduction in N removal. In freshwater marshes, we expect a decreasee in N removal efficiency due to increased sulfide concentrations. Sulfide encroachment will increase the relative importance of dissimilatory nitrate reduction to ammonium and lead to greater bacterial nitrogen immobilization, ultimately resulting in an ecosystem that retains more N and is less effective at permanent N removal from the watershed. In contrast, we predict that sealevel–driven expansion of the tidal creek network and the degree of surface-subsurface exchange flux through tidal creek banks will result in greater N-removal efficiency from these locations.

Book chapter↗

A synthesis of terrestrial mercury in the western United States: Spatial distribution defined by land cover and plant productivity

A synthesis of published vegetation mercury (Hg) data across 11 contiguous states in the western United States showed that aboveground biomass concentrations followed the order: leaves (26 μg kg − 1 ) ~ branches (26 μg kg − 1 ) > bark (16 μg kg − 1 ) > bole wood (1 μg kg − 1 ). No spatial trends of Hg in aboveground biomass distribution were detected, which likely is due to very sparse data coverage and different sampling protocols. Vegetation data are largely lacking for important functional vegetation types such as shrubs, herbaceous species, and grasses. Soil concentrations collected from the published literature were high in the western United States, with 12% of observations exceeding 100 μg kg − 1 , reflecting a bias toward investigations in Hg-enriched sites. In contrast, soil Hg concentrations from a randomly distributed data set (1911 sampling points; Smith et al., 2013a) averaged 24 μg kg − 1 (A-horizon) and 22 μg kg − 1 (C-horizon), and only 2.6% of data exceeded 100 μg kg − 1 . Soil Hg concentrations significantly differed among land covers, following the order: forested upland > planted/cultivated > herbaceous upland/shrubland > barren soils. Concentrations in forests were on average 2.5 times higher than in barren locations. Principal component analyses showed that soil Hg concentrations were not or weakly related to modeled dry and wet Hg deposition and proximity to mining, geothermal areas, and coal-fired power plants. Soil Hg distribution also was not closely related to other trace metals, but strongly associated with organic carbon, precipitation, canopy greenness, and foliar Hg pools of overlying vegetation. These patterns indicate that soil Hg concentrations are related to atmospheric deposition and reflect an overwhelming influence of plant productivity — driven by water availability — with productive landscapes showing high soil Hg accumulation and unproductive barren soils and shrublands showing low soil Hg values. Large expanses of low-productivity, arid ecosystems across the western U.S. result in some of the lowest soil Hg concentrations observed worldwide.

Science of the Total Environment↗

Soil bacterial and fungal community responses to nitrogen addition across soil depth and microhabitat in an arid shrubland

Arid shrublands are stressful environments, typified by alkaline soils low in organic matter, with biologically-limiting extremes in water availability, temperature, and UV radiation. The widely-spaced plants and interspace biological soil crusts in these regions provide soil nutrients in a localized fashion, creating a mosaic pattern of plant- or crust-associated microhabitats with distinct nutrient composition. With sporadic and limited rainfall, nutrients are primarily retained in the shallow surface soil, patterning biological activity. We examined soil bacterial and fungal community responses to simulated nitrogen (N) deposition in an arid Larrea tridentata - Ambrosia dumosa field experiment in southern Nevada, USA, using high-throughput sequencing of ribosomal RNA genes. To examine potential interactions among the N application, microhabitat and soil depth, we sampled soils associated with shrub canopies and interspace biological crusts at two soil depths (0–0.5 or 0–10 cm) across the N-amendment gradient (0, 7, and 15 kg ha −1 yr −1 ). We hypothesized that localized compositional differences in soil microbiota would constrain the impacts of N addition to a microhabitat distribution that would reflect highly localized geochemical conditions and microbial community composition. The richness and community composition of both bacterial and fungal communities differed significantly by microhabitat and with soil depth in each microhabitat. Only bacterial communities exhibited significant responses to the N addition. Community composition correlated with microhabitat and depth differences in soil geochemical features. Given the distinct roles of soil bacteria and fungi in major nutrient cycles, the resilience of fungi and sensitivity of bacteria to N amendments suggests that increased N input predicted for many arid ecosystems could shift nutrient cycling toward pathways driven primarily by fungal communities.

Frontiers in Microbiology↗

Divergent biotic and abiotic filtering of root endosphere and rhizosphere soil fungal communities along ecological gradients

Plant roots assemble two distinct microbial compartments: the rhizosphere (microbes in soil surrounding roots) and the endosphere (microbes within roots). Our knowledge of fungal community assembly in these compartments is limited, especially in wetlands. We tested the hypothesis that biotic factors would have direct effects on rhizosphere and endosphere assembly, while abiotic factors would have direct and indirect effects. Using a field study, we examined the influences of salinity, water level and biotic factors on baldcypress (Taxodium distichum) fungal communities. We found that endosphere fungi were correlated with host density and canopy cover as opposed to rhizosphere, suggesting that hosts can impose selective filters on fungi colonizing into their roots. Meanwhile, local abiotic conditions strongly influenced both rhizosphere and endosphere diversity in opposite patterns: e.g. highest endosphere diversity (hump-shaped) while lowest rhizosphere diversity (U-shaped) at intermediate salinity levels. These results indicate that the assembly and structure for the root endosphere and rhizosphere within a host can be shaped by different processes. Our results also highlight the importance of assessing how environmental changes affect plant and plant-associated fungal communities in wetland ecosystems where saltwater intrusion and sea level rise are major threats to both plant and fungal communities.

FEMS Microbiology Ecology↗

Assessment of rangeland ecosystem conditions in Grand Canyon-Parashant National Monument, Arizona

Sustainability of dryland ecosystems depends on the functionality of soil-vegetation feedbacks that affect ecosystem processes, such as nutrient cycling, water capture and retention, soil erosion and deposition, and plant establishment and reproduction. Useful, common indicators can provide information on soil and site stability, hydrologic function, and biotic integrity. Evaluation of rangeland health thus relies on describing the condition and sustainability of these individual, measurable, and observable indicators that are linked to important ecosystem processes. This report focuses on the ~200,000 acres of the Grand Canyon-Parashant National Monument that is administered by the National Park Service (NPS)—one of the largest NPS units where livestock grazing is a permitted land-use activity. Many ecosystems in the monument are characterized by a low degree of resilience to improper grazing because of low and variable precipitation. The monument is marked by a high degree of environmental heterogeneity, including a large elevation gradient, widely differing precipitation patterns, a diversity of geologic substrates, and unique combinations of plant species. The objective of this report is to (1) increase our understanding of the underlying landscape, soil, and climate setting factors that affect Grand Canyon-Parashant National Monument dryland ecosystem structure and function (also referred to as land potential) and (2) characterize the condition of monument ecosystems in relation to management concepts, such as rangeland health. Data were analyzed by elevation zone using both univariate and multivariate approaches. Survey results document the high level of diversity within the study area, including 15 unique soil taxa and 271 species of plants. We collected three new plant species for Grand Canyon-Parashant National Monument and 17 new species for the NPS portion of the monument. Results also document a strong association between rangeland health indicators and elevation, topographic setting, and soils. Soil factors found to explain important variation across plots include the amount of exposed bedrock, soil rockiness, soil texture (and associated hydrologic properties), and soil depth. We also found that dominant species turnover across elevation may represent species’ differences in adaptation to climates, including Larrea tridentata , Coleogyne ramosissima , and Artemisia spp. Bromus rubens is the most common invasive species of concern recorded in this study, but other common invasive species are Bromus tectorum , Erodium cicutarium , and Schismus arabicus . Correlations between an index of cattle use and indicators of rangeland health suggest that areas with high cattle use have increased bare ground, decreased ground cover, increased frequency of Schismus arabicus , decreased cover of Coleogyne ramosissima and Ephedra spp., and increased cover of Gutierrezia spp. The few strong correlations observed between indicators of vascular plant community cover or abundance and indicators of cattle activity support rangeland assessment and monitoring strategies that do not rely solely on plant-based indicators are needed. This work supports management of dryland ecosystems, including Grand Canyon-Parashant National Monument, using concepts of land potential. We conclude the report with recommendations on improving existing land-potential-based classification systems, associated interpretations, and methods for moving forward with a Grand Canyon-Parashant National Monument rangeland monitoring program.

Arizona↗