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Regional brain morphometry and lissencephaly in the Sirenia

Neuroanatomical structure was examined in the brains of West Indian manatees (Trichechus manatus) using computer-based morphometric methods. Although manatees have a small relative brain size, volume estimates of the major brain regions indicate that the telencephalon comprises 71% of totalbrain volume and is 90% cerebral cortex. These values are comparable to those seen among a diversity of taxa having large relative brain size, including many primates. Manatee brains also exhibit well-defined cortical lamination. The measured gyration index (an index of cortical folding) was 1.06, representing a highly lissencephalic condition. These findings demonstrate that small relative brain size and lissencephaly do not constrain the elaboration of internal brain structures. The marked lissencephalic condition is unusual for brains of this absolute size range, and may be related to the thickness of the cortical gray matter and underlying white matter.

Brain, Behavior and Evolution

Fatal infection with Versteria sp. in a muskrat, with implications for human health

The Taeniidae tapeworms are a family of helminths that have a similar life cycle, with intermediate hosts developing characteristic cysts in visceral organs. We describe here a case in Pennsylvania, USA, of fatal Versteria infection in a muskrat ( Ondatra zibethicus ), which, to our knowledge, has not been reported to develop disease associated with infection. Postmortem examination revealed widespread tissue loss and replacement by solid-bodied cestode larvae with minimal adjacent inflammation in many visceral organs, most severe in the lungs, liver, and brain. Key morphologic features via histology included cephalic structures and short rostellar hooklets, which are characteristic for the genus. Genetic characterization confirmed the cestode as being an undescribed lineage of Versteria that has been implicated as the cause of severe morbidity and mortality in humans and nonhuman primates in North America. Considering the zoonotic significance of this pathogen, our report expands on the limited literature regarding disease caused by Versteria and emphasizes the need to identify the causative tapeworm more accurately, especially in rodent intermediate hosts given that previous reports do not have molecular confirmation of species.

Pennsylvania

Zoonotic viruses associated with illegally imported wildlife products

The global trade in wildlife has historically contributed to the emergence and spread of infectious diseases. The United States is the world's largest importer of wildlife and wildlife products, yet minimal pathogen surveillance has precluded assessment of the health risks posed by this practice. This report details the findings of a pilot project to establish surveillance methodology for zoonotic agents in confiscated wildlife products. Initial findings from samples collected at several international airports identified parts originating from nonhuman primate (NHP) and rodent species, including baboon, chimpanzee, mangabey, guenon, green monkey, cane rat and rat. Pathogen screening identified retroviruses (simian foamy virus) and/or herpesviruses (cytomegalovirus and lymphocryptovirus) in the NHP samples. These results are the first demonstration that illegal bushmeat importation into the United States could act as a conduit for pathogen spread, and suggest that implementation of disease surveillance of the wildlife trade will help facilitate prevention of disease emergence.

PLoS ONE

Repertoire, structure, and individual variation of vocalizations in the sea otter

Vocalizations of the California sea otter ( Enhydra lutris nereis ) were recorded from wild and captive adults and young and analyzed spectrographically. Parameters measured from the sonagrams included fundamental frequency, duration, maximum frequency, intercall interval, and the location and amplitude of energy peaks. We identified 10 basic vocal categories, one of which consisted of graded signals. The contexts for each call, when known or suspected, are described. Discriminant analysis of the spectrographic parameters for the scream call showed significant differences among individuals for adult females and young. Using only the parameters quantified, each call was assigned correctly to the individual that produced it with 80% accuracy for mothers and 75% for young, thus, indicating that the potential exists for individual vocal recognition in the sea otter. The sea otter's vocal repertoire is similar in complexity to that of certain pinnipeds, but may be less complex than that of several species of social cetaceans and primates. In general, the sea otter's vocal patterns have characteristics thought to be most suitable for short-range communication among familiar individuals.

Journal of Mammalogy

Global conservation status of turtles and tortoises (order Testudines)

We present a review and analysis of the conservation status and International Union for Conservation of Nature (IUCN) threat categories of all 360 currently recognized species of extant and recently extinct turtles and tortoises (Order Testudines). Our analysis is based on the 2018 IUCN Red List status of 251 listed species, augmented by provisional Red List assessments by the IUCN Tortoise and Freshwater Turtle Specialist Group (TFTSG) of 109 currently unlisted species of tortoises and freshwater turtles, as well as re-assessments of several outdated IUCN Red List assessments. Of all recognized species of turtles and tortoises, this combined analysis indicates that 20.0% are Critically Endangered (CR), 35.3% are Critically Endangered or Endangered (CR+EN), and 51.9% are Threatened (CR+EN+Vulnerable). Adjusting for the potential threat levels of Data Deficient (DD) species indicates that 56.3% of all data-sufficient species are Threatened. We calculated percentages of imperiled species and modified Average Threat Levels (ATL; ranging from Least Concern = 1 to Extinct = 8) for various taxonomic and geographic groupings. Proportionally more species in the subfamily Geoemydinae (Asian members of the family Geoemydidae) are imperiled (74.2% CR+EN, 79.0% Threatened, 3.89 ATL) compared to other taxonomic groupings, but the families Podocnemididae, Testudinidae, and Trionychidae and the superfamily Chelonioidea (marine turtles of the families Cheloniidae and Dermochelyidae) also have high percentages of imperiled species and ATLs (42.9–50.0% CR+EN, 73.8–100.0% Threatened, 3.44–4.06 ATL). The subfamily Rhinoclemmydinae (Neotropical turtles of the family Geoemydidae) and the families Kinosternidae and Pelomedusidae have the lowest percentages of imperiled species and ATLs (0%–7.4% CR+EN, 7.4%–13.3% Threatened, 1.65–1.87 ATL). Turtles from Asia have the highest percentages of imperiled species (75.0% CR+EN, 83.0% Threatened, 3.98 ATL), significantly higher than predicted based on the regional species richness, due to much higher levels of exploitation in that geographic region. The family Testudinidae has the highest ATL (4.06) of all Testudines due to the extinction of several species of giant tortoises from Indian and Pacific Ocean islands since 1500 CE. The family Testudinidae also has an ATL higher than all other larger polytypic families (≥ 5 species) of Reptilia or Amphibia. The order Testudines is, on average, more imperiled than all other larger orders (≥ 20 species) of Reptilia, Amphibia, Mammalia, or Aves, but has percentages of CR+EN and Threatened species and an ATL (2.96) similar to those of Primates and Caudata (salamanders).

Chelonian Conservation and Biology

Mammals of the Braulio Carrillo- La Selva Complex, Costa Rica

Costa Rica's La Selva-Braulio Carrillo complex encompasses a 60-km protected corridor of Caribbean rain and cloud forest extending from 30 m at the La Selva Biological Station to 2,906 m at the top of Volcán Barva. The 52,000-ha complex covers four life zones and two transitional zones, including tropical wet forest, tropical wet forest cool-transition, tropical premontane wet-transition rain forest, tropical premontane rain forest, lower montane rain forest, and montane rain forest. Located in the northeastern part of the country, the area is representative of Central American Caribbean slope forests that extend from Mexico to Panama. The extensive elevational gradient of the complex provides protected habitat for a variety of altitudinal migrants. With support from the National Geographic Society and Rice Foundation, the Organization for Tropical Studies organized a biological survey of the complex in early 1986. The mammal team worked at six sites along the elevational transect established by the expedition: 300 m, 700 m, 1,000 m, 1,500 m, 2,050 m, and 2,600 m. We supplemented our collecting records with unpublished records made available by colleagues, records in the published literature, and specimens in museum collections. In addition, observations recorded by a variety of observers at the La Selva Biological Station are summarized. The mammal fauna of the complex comprises 142 species including 79 bats, 23 rodents, 15 carnivores, 7 marsupials, 6 edentates, 4 artiodactyls, 3 primates, 2 rabbits, 2 shrews, and 1 perissodactyl. At least 10 additional species are likely to occur there. The only species of mammal likely to have been extirpated from the area is the giant anteater. Recognizing the importance of the area to wildlife and to mankind in general, the government of Costa Rica added 13,500 ha to the complex on 13 April 1986. This area, previously known as the “Zona Protectora,” provided the mid-elevational link between the lowlands of the La Selva Biological Station and the montane forests of Braulio Carrillo National Park. Unfortunately, destruction of the rain forests surrounding the complex will soon render it an isolated island of protected forest. Thus, the area will become increasingly valuable as a refuge for many species with home ranges that require extensive tracts of undisturbed habitat.

North American Fauna

Molecular evolution of TRPC4 regulatory sequences supports a role in mammalian thermoregulatory adaptation

Background Proteins encoded by the canonical transient receptor potential (Trpc) gene family form transmembrane channels involved in diverse signal-transduction pathways. Trpc4 has been shown necessary for the induction of nonshivering thermogenesis (NST) in mice, a key component of which is thermogenic brown adipose tissue (BAT). In bats, T rpc4 exhibited diversifying selection within exons encoding regulatory binding sites of TRPC4. Methods To assess whether diversification of these regulatory sequences mirrors the diversification of mammalian thermoregulatory strategies, the ratio of nonsynonymous to synonymous substitutions (ω) was estimated for multiple tetrapod outgroups and eutherian orders. Four questions were addressed: (1) Did the ancestral eutherian Trpc4 diverge under positive selection from nonplacental mammals that lack BAT? (2) Did Trpc4 subsequently become more constrained in descendant eutherian clades? (3) In eutherian clades that subsequently lost BAT by inactivation of the thermogenin gene Ucp1 , did Trpc4 become less constrained? (4) Does the evolutionary rate of Trpc4 differ between quantitatively more heterothermic mammal orders (bats and rodents) relative to quantitatively less heterothermic outgroups (carnivores, artiodactylids, and primates)? Results Coincident with the advent of BAT, Trpc4 evolutionary rate increased significantly in ancestral eutheria after their divergence from nonplacental mammals but a branch-site model did not support a rate class ω > 1 along that branch. In descendant eutherian mammals, Trpc4 became far more constrained, with an evolutionary rate less than half that of tetrapod clades lacking NST, a pattern was not seen in other Trp channel genes. Intensifying selection in descendent eutherian mammals was further supported with the RELAX program, which also indicated reduced constraint on Trpc4 in clades that have secondarily lost BAT. However, no consistent pattern was identified within mammalian orders with strong variation in heterothermy: evidence of increased evolutionary rate was again found in bats for Trpc4 as well as homologs it directly binds in heteromeric membrane channels ( Trpc5 and Trpc1 ), yet all rodent Trpc genes had low evolutionary rates. Evolutionary rates of Trpc4 and Trpc1 in bats were consistent with relaxed constraint whereas bat Trpc5 experienced diversifying selection. Most variation among tetrapod TRPC4 sequences lies within an 85 amino-acid window that is functionally uncharacterized. Sequence alignments demonstrated that the TRPC4 β isoform, which lacks a portion of the C-terminal regulatory region, originated in basal eutherians but appears to be lost in many tip lineages. Collectively, the data indicate that the C-terminal region of TRPC4 has responded to selection on NST thermoregulation during the diversification of eutherian mammals. The drivers of increased diversification of Trpc4 and interacting genes in bats remain to be determined.

PeerJ

Fatal suppurative nephritis caused by Pseudomonas in a chimpanzee

Reports of nephritis in chimpanzees are relatively rare, compared with those in other nonhuman primates. McClure and Guilloud reported chronic pyelonephritis in a 35-year-old female chimpanzee; Schmidt and Butler reported glomerulonephritis in an 11-year-old female chimpanzee, and Kim reported on a 12-year-old male with subacute interstitial nephritis in a chimpanzee after the animal had recurrent hemolysis due to phenolic intoxication. The present report deals with supprative nephritis caused by Pseudomonas resulting in renal failure in a chimpanzee.

Journal of the American Veterinary Medical Associa

Geophagy by yellowstone grizzly bears

We documented 12 sites in the Yellowstone ecosystem where grizzly bears ( Ursus arctos horribilis ) had purposefully consumed soil (an activity known as geophagy). We also documented soil in numerous grizzly bear feces. Geophagy primarily occurred at sites barren of vegetation where surficial geology had been modified by geothermal activity. There was no evidence of ungulate use at most sites. Purposeful consumption of soil by bears peaked first from March to May and again from August to October, synchronous with peaks in consumption of ungulate meat and mushrooms. Geophageous soils were distinguished from ungulate mineral licks and soils in general by exceptionally high concentrations of potassium (K) and high concentrations of magnesium (Mg) and sulphur (S). Our results do not support the hypotheses that bears were consuming soil to detoxify secondary compounds in grazed foliage, as postulated for primates, or to supplement dietary sodium, as known for ungulates. Our results suggest that grizzly bears could have been consuming soil as an anti-diarrheal.

Yellowstone National Park

Indopithecus giganteus distinct from Sivapithecus indicus

The very large Eurasian Miocene ape Indopithecus giganteus is distinct from contemporanious Sivapithecus (non-Dryopithecus)indicus. The probabilities that length and width for the only specimen of I. giganteus could be sampled from populations similar or identical to those of S. indicus are less than six chances in 100,000 for both parameters. ?? 1980 Japan Monkey Centre.

Primates

Ecological survey of the night monkey, Aotus trivirgatus, in Formosa Province, Argentina

Transect surveys were carried out in northern Argentina during October and November 1977 in order to determine the distribution and abundance of Aotus trivirgatus . The monkeys were seen in pairs with one to two recent young and occurred at a density of approximately six family groups/km 2 . Aotus was only found in relatively moist, riparian, low forests. Some life history traits, such as diurnal activity and the lack of tree-hole use, are distinctive compared to more northern populations.

Formosa Province

Capturing and marking howler monkeys for field behavioral studies

Methods for capturing and marking howler monkeys for ecological studies are discussed. Systems for capturing and handling animals are compared. A dart with liquid Sernylan for capture and Sernylan or Ketamine as a holding drug was preferred to darts using powdered succinylcholine chloride (SCC) and ether. The effectiveness of both Sernylan and SCC is compared and dosages are given for Sernylan in howler monkeys and SCC for howlers and capuchins. The advantages of Ketamine over ether as a holding drug are discussed. Animals can be marked with leg-bands, collars, and freeze-branding.

Primates